Convulsive Activity Research Articles

Recording of hippocampal activity on the effect of convulsant doses of caffeine

Seizures occur when there is a hyper-excitation of the outer layer of the brain, with subsequent excessive synchrony in a group of neurons. According to the World Health Organization (WHO), an estimated 50 million people are affected by this disease, a third of whom are resistant to the treatments available on the market. Caffeine (1,3,7-trimethylxanthine), which belongs to the purine alkaloid family, is the most widely consumed psychoactive drug in the world. It is ingested by people through drinks containing this substance, such as coffee, and as an adjuvant in analgesic therapy with non-steroidal antiflammatory drugs. The present study evaluated the electrocorticographic changes observed in the hippocampus of Wistar rats subjected to acute doses of caffeine (150 mg/kg i.p), which represents a toxic dose of caffeine corresponding to an estimated acute intake of more than 12 cups of coffee to record its convulsant activity. Our results showed, for the first time, that the administration of high doses of caffeine (150 mg/kg i.p.) in rats caused an increase in the spectral distribution of power in all frequency bands and suggested the appearance of periods of ictal and interictal peaks in the electrocorticogram (ECog). We have also shown that the anticonvulsants phenytoin, diazepam and phenobarbital have a satisfactory response when associated with caffeine.

Synthesis, Characterization and In-Vivo Anti Convulsant Activity of "Benzimidazole and Its Derivatives by Using Aqueous Extract of Acacia Concinna Pods (20% w/v)"

Synthesis, Characterization and In-Vivo Anti Convulsant Activity of "Benzimidazole and Its Derivatives by Using Aqueous Extract of Acacia Concinna Pods (20% w/v)"

From bedside to bench: New insights in epilepsy-associated tumors based on recent classification updates and animal models on brain tumor networks.

Low-grade neuroepithelial tumors (LGNTs), particularly those with glioneuronal histology, are highly associated with pharmacoresistant epilepsy. Increasing research focused on these neoplastic lesions did not translate into drug discovery; and anticonvulsant or antitumor therapies are not available yet. During the last years, animal modeling has improved, thereby leading to the possibility of generating brain tumors in mice mimicking crucial genetic, molecular and immunohistological features. Among them, intraventricular in utero electroporation (IUE) has been proven to be a valuable tool for the generation of animal models for LGNTs allowing endogenous tumor growth within the mouse brain parenchyma. Epileptogenicity is mostly determined by the slow-growing patterns of these tumors, thus mirroring intrinsic interactions between tumor cells and surrounding neurons is crucial to investigate the mechanisms underlying convulsive activity. In this review, we provide an updated classification of the human LGNT and summarize the most recent data from human and animal models, with a focus on the crosstalk between brain tumors and neuronal function.

Peripherally-derived LGI1-reactive monoclonal antibodies cause epileptic seizures in vivo.

One striking clinical hallmark in patients with autoantibodies to leucine-rich glioma inactivated 1 (LGI1) is the very frequent focal seizure semiologies, including faciobrachial dystonic seizures (FBDS), in addition to the amnesia. Polyclonal serum IgGs have successfully modelled the cognitive changes in vivo but not seizures. Hence, it remains unclear whether LGI1-autoantibodies are sufficient to cause seizures. We tested this with the molecularly precise monoclonal antibodies directed against LGI1 [LGI1-monoclonal antibodies (mAbs)], derived from patient circulating B cells. These were directed towards both major domains of LGI1, leucine-rich repeat and epitempin repeat, and infused intracerebroventricularly over 7 days into juvenile male Wistar rats using osmotic pumps. Continuous wireless EEG was recorded from a depth electrode placed in hippocampal CA3 plus behavioural tests for memory and hyperexcitability were performed. Following infusion completion (Day 9), post-mortem brain slices were studied for antibody binding and effects on Kv1.1. The LGI1-mAbs bound most strongly in the hippocampal CA3 region and induced a significant reduction in Kv1.1 cluster number in this subfield. By comparison to control-Ab injected rats video-EEG analysis over 9 days revealed convulsive and non-convulsive seizure activity in rats infused with LGI1-mAbs, with a significant number of ictal events. Memory was not impaired in the novel object recognition test. Peripherally-derived human LGI1-mAbs infused into rodent CSF provide strong evidence of direct in vivo epileptogenesis with molecular correlations. These findings fulfill criteria for LGI1-antibodies in seizure causation.

Wireless EEG Recording of Audiogenic Seizure Activity in Freely Moving Krushinsky-Molodkina Rats.

This study investigates audiogenic epilepsy in Krushinsky-Molodkina (KM) rats, questioning the efficacy of conventional EEG techniques in capturing seizures during animal restraint. Using a wireless EEG system that allows unrestricted movement, our aim was to gather ecologically valid data. Nine male KM rats, prone to audiogenic seizures, received implants of wireless EEG transmitters that target specific seizure-related brain regions. These regions included the inferior colliculus (IC), pontine reticular nucleus, oral part (PnO), ventrolateral periaqueductal gray (VLPAG), dorsal area of the secondary auditory cortex (AuD), and motor cortex (M1), facilitating seizure observation without movement constraints. Our findings indicate that targeted neural intervention via electrode implantation significantly reduced convulsive seizures in approximately half of the subjects, suggesting therapeutic potential. Furthermore, the amplitude of brain activity in the IC, PnO, and AuD upon audiogenic stimulus onset significantly influenced seizure severity and nature, highlighting these areas as pivotal for epileptic propagation. Severe cases exhibited dual waves of seizure generalization, indicative of intricate neural network interactions. Distinctive interplay between specific brain regions, disrupted during convulsive activity, suggests neural circuit reconfiguration in response to escalating seizure intensity. These discoveries challenge conventional methodologies, opening avenues for novel approaches in epilepsy research and therapeutic interventions.

THE SAFFRON EFFECTS ON THE DYNAMICS OF EXPERIMENTAL EPILEPSY

The scientific work is devoted to the influence of saffron extract on the dynamics of the formation of experimental epilepsy. The dynamics of amygdala-like epileptogenesis created by penicillin sodium salt has been studied, with the registration of electrical activity in the structures of the visual system. The exsperiments was carried out on rabbits. The electrical activity of the structures of the visual system was registered by electroencephalography. Recorded electrical activity of the brain in the central and subcortical structures of the visual analyzer (visual cortex, colliculus superior, lateral geniculata body) normally, as well as against the background of the development of epilepsy. As a result of the research, it was found that the introduction of penicillin solution into the amygdala of animals led to the development of prolonged convulsive activity. To determine the effect of saffron extract on the dynamics of amygdala epileptogenesis, a comparative analysis of the dynamics of epilepsy in control and saffron-treated animals was performed based on several parameters. The results obtained give reason to speak about the presence of the effect of the clarifying effect of saffron on the dynamics of epileptogenesis.

Evaluation of In-vivo Anti-Depressant, Anti-Anxiety and Anti-Convulsant Activity of Ficus Lacor Bark

Background and Objectives: Ficus lacor is a large deciduous, rapidly growing closely foliaceous tree about 20 m in height with a fine shaped crown. It is widely distributed in tropical and subtropical regions of the world. This plant is mentioned in different ayurvedic texts with different therapeutic values and literatures. Ficus lacor is used for its anti-inflammatory, anti-arthritic, antioxidant and anti-diabetic activities from time era. The present study was conducted to evaluate Antidepressant, Anxiolytic and Anticonvulsant activity of Ficus lacor bark using various animal models. Materials and Methods: Wistar rats of either sex weighing 150-250 gram were used in this study. Extract was studied for acute toxicity study. Ethanolic extract of low dose (250 mg/kg) and high dose (500 mg/kg) were performed for anti-depressant, anxiolytic and anti-convulsant activity. Antidepressant and anxiolytic activity were performed by Rota-rod and elevated plus maze. Anti-convulsant activity was studied in isoniazid induced convulsion in Wistar rat. Results: Extract treated 500 mg/kg showed more effective and significant (P<0.05) anti-depressant activity. Extract treated group with 500 mg/kg and 250 mg/kg showed significant anxiolytic activity in both No. of open arm entries and time spent in open arm but have no significant anti- convulsant activity. Conclusion: The study concludes that the ethanolic extract possesses anti-depressant and anxiolytic activity of Ficus lacor bark but have no significant anti-convulsant activity in isoniazid induced convulsion. Our study will aware the community people for the preservation of this medicinal plant and will reduce the wastage of drug by proper utilization of the crude drug.

Prevalence of nonconvulsive seizures and nonconvulsive status epilepticus in dogs and cats with a history of cluster seizures: A retrospective study.

Nonconvulsive seizures (NCS) and nonconvulsive status epilepticus (NCSE) are frequently observed in human patients. Diagnosis of NCS and NCSE only can be achieved by the use of electroencephalography (EEG). Electroencephalographic monitoring is rare in veterinary medicine and consequently there is limited data on frequency of NCS and NCSE. Determine the prevalence of NCS and NCSE in dogs and cats with a history of cluster seizures. Twenty-six dogs and 12 cats. Retrospective study. Medical records of dogs and cats with cluster seizures were reviewed. Electroencephalography was performed in order to identify electrographic seizure activity after the apparent cessation of convulsive seizure activity. Nonconvulsive seizures were detected in 9 dogs and 2 cats out of the 38 patients (29%). Nonconvulsive status epilepticus was detected in 4 dogs and 2 cats (16%). Five patients had both NCS and NCSE. A decreased level of consciousness was evident in 6/11 patients with NCS, 3/6 also had NCSE. Mortality rate for patients with NCS (73%) and NCSE (67%) was much higher than that for patients with no seizure activity on EEG (27%). Prevalence of NCS and NCSE is high in dogs and cats with a history of cluster seizures. Nonconvulsive seizures and NCSE are difficult to detect clinically and are associated with higher in hospital mortality rates. Results indicate that prompt EEG monitoring should be performed in dogs and cats with cluster seizures.

Specificity of seizure genesis during electroconvulsive therapy and modified pentylenetetrazole kindling

Comparison of the development of chemically induced seizures in the experiment with the change in convulsive thresholds during electroconvulsive therapy (ECT) has demonstrated that repeated ‘chemical’ seizures can initiate the development of the kindling phenomenon, but regular ECT from the first to the 14th session most likely cause an increase in convulsive thresholds. However, the repeated ECT over 15 sessions is associated with a rapid decrease in the threshold current dose and probable dysregulation of endogenous anticonvulsant mechanisms, with the risk of the development of uncontrolled paroxysmal conditions and the risk of organic CNS lesions. The mechanisms of the convulsive action of ECT and pentylenetetrazolinduced kindling are fundamentally different. Differences in the pathogenesis of systemic convulsive reactions determine the divergent change in seizure thresholds during ECT and pentylenetetrazol stimulations.

Study of the anticonvulsant activity of the original valproic acid amino ester in various models of convulsive syndrome

Introduction. Despite a significant number of antidotes (atropine, pyridoxine) and anti-seizure drugs in clinical practice (benzodiazepines, barbiturates, valproates, anesthetics), the development of new antidotes for the relief of refractory seizures and recurrence of convulsive syndrome is actual problem. 
 The aim of the work was to study the specific pharmacological activity of a new original derivative of valproic acid in an experiment on models of convulsive syndrome with different mechanisms of action.
 Material and methods. A comparative study of the effectiveness of the original substance ((1-methylpiperidin-4-yl)-2-propylpentanoate hydrochloride, VAA) was carried out on electroshock, GABA-lytic, catecholamine-dependent seizure models in rats and mice.
 Results. A significant decrease in seizure activity was revealed in the group with prophylactic administration 
 of VAA in the dose range of 11, 27 and 65 mg/kg in comparison with the control group received only phenylcarbamate. In an intergroup comparison, a low dose (11 mg/kg) more than others had an effect on the latent period of the onset of phenylcarbamate seizures, an average dose (27 mg/kg) reduced the duration of the seizure period, a high dose (65 mg/kg) affected the severity of convulsions, while the integral indicator (convulsive activity) did not differ between the medium and high dose subgroups. There was no significant decrease in seizure activity in models with the use of corazol, camphor, and the model of maximum electric shock.
 Limitations. Testing of new pharmaceutical substances (study of efficacy and safety) should be carried out on laboratory animals before confirmation of efficacy in humans. The quantity of animals was limited by the need to comply with bioethical principles and sufficient number for statistically significant results and amounted 
 6 individuals in each group.
 Conclusion. The release of valproic acid during the metabolism of VAA is slow and its concentration is insufficient to realize the universal anticonvulsant activity of VAA. It is necessary to further study the effect on cognitive functions and neuroprotective properties. Since antidote efficacy superior to atropine has been previously demonstrated, VAA is recommended for development as an antidote for organophosphorus poisoning.

Strychnine Poisoning: Literature Review

Strychnine poisoning is an quite unusual but a serious poisoning in which convulsions are the major threat to life. Convulsions are predominantly noted at the spinal level, and the key to recognizing this poison is observation of convulsive activity in the awake patient without a postictal phase. Successful treatment requires aggressive airway control and treatment of seizures with benzodiazepines or barbiturates. Neuromuscular blockade may be required. Gastrointestinal decontamination is usually indicated in recent acute ingestions but may precipitate convulsions. Recovery from strychnine poisoning is usually complete and rapid if treatment is aggressive.

The link between seizures and prolactin: A study on the effects of anticonvulsant medications on hyperprolactinemia in rats

Seizures are a common neurological disorder that affects people of all ages. These sudden, uncontrolled electrical disturbances in the brain can cause a variety of symptoms, including convulsions, loss of consciousness, and abnormal sensations. While seizures have long been recognized as a potential cause of hormonal imbalances, recent research has shed new light on the link between seizures and prolactin. The study involved 30 adult female Wistar rats, which were divided into a control group (treated with normal saline) and four treatment groups: chronic group (treated with 30 mg/kg pentylenetetrazol intraperitoneally three days a week for 10 weeks), chronic + Levetiracetam (50 mg/kg, gavage), chronic + Cabergoline (0.05 mg/kg, gavage), and chronic + Levetiracetam (25 mg/kg) + cabergoline (0.025). The drugs were administered three days a week for 10 weeks. Field action potentials were recorded from the CA1 area of the hippocampus using eLab after anesthetizing the animals with a ketamine-xylazine combination (70 +7 mg/kg). The prolactin levels were measured using the ELISA method after serum preparation. The findings indicate that the use of levetiracetam as an anticonvulsant drug resulted in a significant decrease in the amount of prolactin and spike number of convulsive activities compared to the chronic group. However, the amplitudes of convulsive activities did not show a significant difference between the control and other treatment groups. In conclusion, investigating the possibility of subclinical seizures and utilizing anticonvulsant medications in hyperprolactinemia that is resistant to treatment are crucial in treating infertility.

Peculiarities of the effect of antiepileptic drugs on seizures in mice with corneal kindling against the background of low-dose premedication with carbamazepine and sulthiame

The aim. The aim of the study was to evaluate the effectiveness of antiepileptic drugs (AED) with a different mechanism of action against the background of prior use of low doses of carbamazepine and sultiam in the model of corneal kindling in mice. Materials and methods. The corneal kindling model in mice was used. The convulsive model was reproduced under the parallel preliminary administration of carbamazepine and sulthiame 30 minutes before electrostimulation. The anticonvulsant activity of AED (sulthiame, levetiracetam, carbamazepine, valproate, lamotrigine and retigabine) was studied under conditions after the formation of a stable syndrome of the generalised convulsive activity. Results and discussion. The administration of carbamazepine and sulthiame drugs modulates the activity of the cytochrome P450 enzyme system. Thus, carbamazepine in the dose of 7 and 12 mg/kg showed no significant anticonvulsant activity (the convulsive intensity – 4.42±0.25 points; 4.44±0.32 points) after its preliminary chronic administration, the same doses of carbamazepine showed a noticeable anticonvulsant effect in the control group of animals (3.52±0.26 points; 3.2±0.6 points, respectively). The anticonvulsant activity of lamotrigine changed both in the case of the preliminary chronic administration of an inducer (carbamazepine), and an inhibitor (sulthiame) of the cytochrome P450 system. Conclusion. Changes in the pharmacological effects of AED observed against the background of chronic administration of carbamazepine and sulthiame, in our opinion, may be due to both the modulation of the cytochrome P450 system and other groups of enzymes involved in the AED metabolism

Nonconvulsive Status Epilepticus: A Review for Emergency Clinicians

BackgroundStatus epilepticus is associated with significant morbidity and mortality and is divided into convulsive status epilepticus and nonconvulsive status epilepticus (NCSE). ObjectiveThis review provides a focused evaluation of NCSE for emergency clinicians. DiscussionNCSE is a form of status epilepticus presenting with prolonged seizure activity. This disease is underdiagnosed, as it presents with nonspecific signs and symptoms, most commonly change in mental status without overt convulsive motor activity. Causes include epilepsy, cerebral pathology or injury, any systemic insult such as infection, and drugs or toxins. Mortality is primarily related to the underlying condition. Patients most commonly present with altered mental status, but other signs and symptoms include abnormal ocular movements and automatisms such as lip smacking or subtle motor twitches in the face or extremities. The diagnosis is divided into electrographic and electroclinical, and although electroencephalogram (EEG) is recommended for definitive diagnosis, emergency clinicians should consider this disease in patients with prolonged postictal state after a seizure with no improvement in mental status, altered mental status with acute cerebral pathology (e.g., stroke, hypoxic brain injury), and unexplained altered mental status. Assessment includes laboratory evaluation and neuroimaging with EEG. Management includes treating life-threatening conditions, including compromise of the airway, hypoglycemia, hyponatremia, and hypo- or hyperthermia, followed by rapid cessation of the seizure activity with benzodiazepines and other antiseizure medications. ConclusionsAn understanding of the presentation and management of NCSE can assist emergency clinicians in the care of these patients.

Synthesis and Anti Convulsant Activity of "N'-{4-[2-(1h-Benzimidazol-2-Yl)-2-Oxoethyl] Phenyl}-2-Hydroxyacetohydrazide and Its Derivatives"

Introduction: Benzimidazole is a heterocyclic hydrocarbon having unique basic structural characteristics in their structure. This moiety was formed by using aromatic benzene and imidazole heterocylic ring. This molecule gives great application in biological and clinical studies. Benzimidazole and its derivatives have versatile Nitrogen containing heterocyclic compounds that give a promising category of biologically active compounds and possess a wide selection of biological activities like antibacterial drug, medicament, anti-ulcer, anti-diabetic, etc. Objective: Several scientists have declared that benzimidazole system possesses the variable sites like the position of a pair and five which might be fittingly changed to yield potent therapeutic agents. The current review covers the chemistry and medicine activities of substituted benzimidazoles. Materials & Methods: Formic acid; Acetyl Chloride; Hydrazine; Benzene-1,2-diol; Glycolic Acid; Benzoyl Chloride; Methyl Chloride; Ethyl Chloride; Benzamide etc. and methods are TLC, IR spectra, ¹H-NMR and MS. Results & Conclusion: The pharmacological screening was done by using PTZ Induced Convulsion Method for anticonvulsant activity. The synthesize compounds were established to be BA to BK. The compound BA, BC, BJ, BI and BK were established to be the most potent compound through the comparison with standard drugs phenytoin. Synthesized newer benzimidazole derivatives were screened for Anti-convulsant activity. It was seen that in biological activity, derivatives containing 2-nitro aniline and 3-nitro aniline have more significant biological activities than other benzimidazole derivatives. Total 11 compounds (4 Step Products + 7 Benzimidazole Derivatives) were evaluated for their biological screening.

EFFECT OF TAURINE ON MANIFESTATION OF AUDIOGENIC EPILEPSY IN RATS WITH PENDULUM MOVEMENTS

A decrease of taurine in hippocampus of the line of rats with pendulum movements (PM), predisposed to audiogenic epilepsy, was shown. Taurine (aminoethanesulfonic acid) is known to be an anticonvulsant drug. The aim of the present work was to verify the predictive validity of taurine in rats with pendulum movements (PM). In neonatal period the exogenous taurine administration did not influence a convulsive activity at the age of 1.5 and 3 months in PM rats. The increase in the number of audiogenic seizures after taurine injections was found in adolescent period in 1.5 months and in prolonged point – in 3 months. The number of abortive seizures decreased under acute injection of taurine in adult PM rats, but the level of ictal and postictal indicators restored one month later, at the age of 4 months. Obtained results indicate the absence of effect of taurine administered to rats in the neonatal age, while at the long-term scale, the rats showed the increasing seizure activity under the action of taurine in adolescent period and confirm the predictive validity of this drug in adult PM rats.

Characterizing candidate decompression rates for hypobaric hypoxic stunning of pigs. Part 1: Reflexive behavior and physiological responses.

Alternatives to carbon dioxide (CO2) stunning for the commercial slaughter of pigs are urgently needed because there is robust evidence that exposing pigs to hypercapnic environments is associated with pain, fear, and distress. Hypobaric hypoxia (via gradual decompression, also known as Low Atmospheric Pressure Stunning or LAPS) has been validated in poultry as a humane option, but its potential to improve the welfare of pigs at slaughter is unknown. We investigated the potential of hypobaric hypoxia to reliably elicit a non-recovery state in anesthetized weaner-grower pigs within a commercially viable timeframe. We determined the effect of candidate decompression rates (40, 60, 80, 100 ms-1, at two cycle durations 480 s and 720 s) on a range of physiological and reflexive behavioral indicators of hypoxia and death. We found that the decompression rates tested caused a 100% death rate. As expected, the decompression rate had overarching effects on behavioral and physiological markers of hypoxia and death, with faster decompression rates resulting in shorter latencies to cardiac arrest and cessation of breathing. We observed a higher proportion of pigs displaying repeated and prolonged whole-body movements (likely indicative of convulsive activity) at higher frequencies when we applied the slowest decompression rate (40 ms-1) compared to all other rates. Since these responses may impact the carcass and meat quality, the slower rate of decompression (40 ms-1) should be excluded as a candidate decompression rate. Furthermore, given the marginal effects of decompression rate on physiological indicators of death and reflexive behavioral parameters, we also recommend that the fastest rate tested (100 ms-1) is excluded in further study on conscious pigs (to prevent conscious animals from being exposed to unnecessary faster decompression rates which may compromise animal welfare). This work represents a necessary proof of principle step and confirms the potential of gradual decompression for stunning purposes in pigs. Importantly, however, the data presented provide no information on the welfare outcomes associated with decompression in conscious pigs. Subsequent work should focus on the comprehensive welfare assessment of intermediate decompression rates to determine the potential of hypobaric hypoxia to provide a humane stunning method for pigs.

Dynamics of Changes in Acetylcholinesterase Activity in the Brain and Bood of Rats During Pharmacological Correction of Seizures Caused by Carbamate

To simulate a convulsive syndrome, phenylcarbamate was administered intraperitoneally at a dose of 1 mg/kg. Valproic acid aminoester (43 mg/kg), caramiphene (50 mg/kg), and diferidine (2 mg/kg) were investigated as potential anticonvulsant drugs. Blood and brain tissue for the determination of acetylcholinesterase (AСhE) were taken at 10, 30, 60 minutes, 6 and 24 hours after administration of the xenobiotic. In case of poisoning with reversible inhibitors of AChE and accumulation of acetylcholine in the CNS synapses (due to inhibition of AChE activity), only the initial manifestations of convulsive activity are caused, and other mechanisms not associated with AChE inhibition are responsible for the processes of further maintenance and recurrence of seizures.

CREATION OF ANTI-INFLAMMATORY PHARMACEUTICAL COMPOSITIONS (review)

The review article summarizes the date of literature and our own research on the creation of more effective and safe domestic combined drugs with a pronounced anti-inflammatory and analgesic effects. Untimely treatment of inflammatory processes often leads to long-term disability and even disability, so today there is an active search for new effective and safe domestic combination drugs with pronounced anti-inflammatory and analgesic effects. The constant search for innovative drugs causes a significant number of diseases, pathological conditions for which treatment with existing drugs is not effective or causes addiction and a large number of side effects. Because of, inflammatory processes can be accompanied by severe pain, spasms, increased convulsive activity of the brain, other disorders of the body, so non-narcotic analgesics and nonsteroidal anti-inflammatory drugs are combined with other drugs that can cause complementary effects or potentiate each other's effects. Caffeine is a useful adjuvant of nonsteroidal anti-inflammatory drugs of various chemical structures in terms of anti-inflammatory and analgesic effects. The review article presents a literature review of the current state of treatment of inflammation and pain, conducting research to expand the therapeutic capabilities of known pharmaceuticals, the creation of pharmaceutical compositions of nonsteroidal anti-inflammatory drugs with caffeine.

Characterisation of NLRP3 pathway-related neuroinflammation in temporal lobe epilepsy.

Inflammation of brain structures, in particular the hippocampal formation, can induce neuronal degeneration and be associated with increased excitability manifesting as propensity for repetitive seizures. An increase in the abundance of individual proinflammatory molecules including interleukin 1 beta has been observed in brain tissue samples of patients with pharmacoresistant temporal lobe epilepsy (TLE) and corresponding animal models. The NLRP3-inflammasome, a cytosolic protein complex, acts as a key regulator in proinflammatory innate immune signalling. Upon activation, it leads to the release of interleukin 1 beta and inflammation-mediated neurodegeneration. Transient brain insults, like status epilepticus (SE), can render hippocampi chronically hyperexcitable and induce segmental neurodegeneration. The underlying mechanisms are referred to as epileptogenesis. Here, we have tested the hypothesis that distinct NLRP3-dependent transcript and protein signalling dynamics are induced by SE and whether they differ between two classical SE models. We further correlated the association of NLRP3-related transcript abundance with convulsive activity in human TLE hippocampi of patients with and without associated neurodegenerative damage. Hippocampal mRNA- and protein-expression of NLRP3 and associated signalling molecules were analysed longitudinally in pilocarpine- and kainic acid-induced SE TLE mouse models. Complementarily, we studied NLRP3 inflammasome-associated transcript patterns in epileptogenic hippocampi with different damage patterns of pharmacoresistant TLE patients that had undergone epilepsy surgery for seizure relief. Pilocarpine- and kainic acid-induced SE elicit distinct hippocampal Nlrp3-associated molecular signalling. Transcriptional activation of NLRP3 pathway elements is associated with seizure activity but independent of the particular neuronal damage phenotype in KA-induced and in human TLE hippocampi. These data suggest highly dynamic inflammasome signalling in SE-induced TLE and highlight a vicious cycle associated with seizure activity. Our results provide promising perspectives for the inflammasome signalling pathway as a target for anti-epileptogenic and -convulsive therapeutic strategies. The latter may even applicable to a particularly broad spectrum of TLE patients with currently pharmacoresistant disease.