Conversion Machinery Research Articles

Comparing the Pressure Containment Requirements of Three Subsea Hydro-Pneumatic Energy Storage Concepts

Abstract Hydro-pneumatic energy storage has significant potential for being deployed subsea and co-located with offshore wind farms. The pressure containment storing the compressed air is the bulkiest and most expensive component of such a storage technology. This paper evaluates three different subsea hydro-pneumatic energy storage concepts, comparing their volumetric storage densities, the steel and concrete anchoring requirements and cost of the pressure containment. The pressure variation experienced by the pressure containment across the storage cycle for the three variants, which would impact the efficiency of energy conversion machinery, is also compared. It is shown that having separate accumulators, each designed for a different operating pressure, offers the potential of reducing the cost of the pressure containment by up to 40% as compared to that for the simple concept having a single accumulator. A novel approach is presented to achieve such cost reduction without constraining the energy conversion machinery to operate over a wider operating pressure range.

How pressure affects costs of power conversion machinery in compressed air energy storage; Part I: Compressors and expanders

This study addresses a critical economic aspect in compressed air energy storage that has not been discussed much in existing literature: the impact of operating pressure on machinery capital cots. It aims to answer whether the cost per unit of power for power conversion systems changes with the maximum storage pressure. Considering that higher storage pressures are associated with greater energy density, enhanced energy storage capabilities and improved system efficiency. This paper helps clarify uncertainties in initial cost estimations for power-generation plants. Effects of operating pressure on the components and overall sizes and consequently costs of power conversion machinery are individually investigated in two parts. Part I encompasses the compressor and expanders, and part II comprehensively discusses the effects of the operating pressure on the costs of heat exchangers. The analysis employs a conceptual engineering approach, revealing that higher intake pressure reduces overall compressor/expander size, leading to cost savings. Additionally, increasing the number of compression stages for higher storage pressures enhances exergy storage cost-effectiveness. To establish an advanced adiabatic CAES plant with a storage pressure of 200 bar instead of 50 bar, there is potential for a 6 % reduction in $/kW expenditure.

A universal hydraulic-mechanical diagnostic framework based on feature extraction of abnormal on-field measurements: Application in micro pumped storage system

How to extract the running feature information and realize multi-type faults diagnosis is the key to carry out intelligent operation and maintenance of energy conversion machinery. The pumped storage unit (PSU) has various operating conditions, both energy storage and power generation. It may lead to diversified types of failures under the joint influence of hydraulic and mechanical factors. The existing data-driven models often show excellent diagnostic performance with laboratory-specific fault types or single subsystem but are not satisfactory when applying real operating scenarios or migrating to other devices. Therefore, a universal hydraulic-mechanical diagnostic framework integrating signal acquisition, feature extraction and fault recognition is proposed in this paper. In Stage 1, eight types of datasets caused by the hydraulic-mechanical coupling characteristics via abnormal on-field measurements form an on-site benchmark; for Stages 2 and 3, a novel refined composite multiscale cosine similarity Lempel-Ziv complexity method is proposed to quantify the various fault features based on multiscale signal processing and the nonlinear dynamics methodology, and random forests model is introduced to realize the efficient recognition of different status signals. Its core advantage is versatility, which is not limited to specific components but can be applied to different subsystems of pumped storage, such as hydraulic system (identification of vortex conditions, detection of hydraulic imbalances) and mechanical system (wear of shaft, bearing and runner). This framework is applied in the micro PSUs, the comprehensive experiments show that all evaluation indexes are above 92%. Various comparative analysis indicates that the framework is not only applicable to the detection and analysis of hydraulic anomalies but also has a competitive advantage in the diagnosis of hydraulic-mechanical faults. It is suitable for fault detection of different subsystems in real power stations, and also could be flexibly extended to other rotating energy storage systems, as a helpful tool to improve energy conversion efficiency and reduce maintenance cost.

Understanding the biomass conversion processes of bovine gut microbiota through community-wide metabolic interaction network

Biofuels derived from plant biomass can serve as an alternative for reducing the global energy demands. However, breakdown of plant biomass requires an energy-intensive pretreatment process. Biomass pretreatment with microbial communities can help for efficient biofuel production. A diverse microbial population residing inside the bovine gut is efficient for the conversion of lignocellulosic biomass. Here, we report a community-wide metabolic interaction network (CMIN) of 223 microbial species identified from the bovine gut environment. CMIN helped to understand the metabolic interactions of cellulolytic, fermentative, and methanogenic microbial species during the conversion of lignocellulosic biomass. Several key lignocellulolytic species have been identified through the interspecies influence network. Moreover, the lignocellulose degradation network represented the sequential events of microbial biomass conversion and their GH enzyme activities. Therefore, the overall interaction study will be effective for understanding the natural biomass conversion machinery in bovine gut.

A portable and quantitative detection of microRNA-21 based on cascade enzymatic reactions with dual signal outputs

MicroRNAs (miRNAs) are physiological status-related molecules which can be used as biomarkers for diseases, such as cancers. The point-of-care testing (POCT) of miRNAs has great application potential in early diagnosis and process monitoring of diseases. In this paper, a fast and dual signal outputs detection for microRNA-21 (miRNA-21) was established by using both personal glucose meter (PGM) and fluorescence spectrometer. In such an assay protocol, a dual-functional hairpin structure was rationally designed to recognize miRNA-21 and serve as the carrier of the reporter adenosine monophosphate (AMP). The hairpin structure can be specifically degraded by exonuclease T (Exo T) after hybridization with the target miRNA-21, releasing a large amount of AMP as the reporter. Then a smart signal conversion machinery composed of four enzymes and the corresponding substrates was employed to produce dual output signals through enzymatic cascade reactions. The machinery includes two parts: an adenosine triphosphate (ATP) generation system and a glucose consumption/NADPH production system. The produced AMP in the former step triggers the production of ATP, and subsequently the consumption of glucose and the production of NADPH. The changes of both glucose and NADPH are proportional to the concentration of miRNA-21, and can be determined by PGM and fluorescence spectrometer, respectively. Besides, the build-in substrate-recycling mechanism achieves signal amplification of the cascade enzymatic reactions. Under the optimal experimental conditions, the PGM signal is linearly correlated with the concentration of miRNA-21 in the range from 5 to 150 nM, with the limit of detection (LOD) of 3.65 nM. The LOD of fluorescence detection mode is even lowered to 0.03 nM. The miRNA-21-spiked serum samples, as well as the actual serum samples from cancer patients, have been successfully detected by this detection strategy. Thus the established assay provides a POCT solution for cancer diagnosis and prognosis.

Beyond 'seeing is believing': the antenna size of the photosystems in vivo.

SummaryPhotosystems I and II are the central components of the solar energy conversion machinery in oxygenic photosynthesis. They are large functional units embedded in the photosynthetic membranes, where they harvest light and use its energy to drive electrons from water to NADPH. Their composition and organization change in response to different environmental conditions, making these complexes dynamic units. Some of the interactions between subunits survive purification, resulting in the well‐defined structures that were recently resolved by cryo‐electron microscopy. Other interactions instead are weak, preventing the possibility of isolating and thus studying these complexes in vitro. This review focuses on these supercomplexes of vascular plants, which at the moment cannot be ‘seen’ but that represent functional units in vivo.

Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol

Methylotuvimicrobium alcaliphilum 20Z is a promising platform strain for bioconversion of one-carbon (C1) substrates into value-added products. To carry out robust metabolic engineering with methylotrophic bacteria and to implement C1 conversion machinery in non-native hosts, systems-level evaluation and understanding of central C1 metabolism in methanotrophs under various conditions is pivotal but yet elusive. In this study, a genome-scale integrated approach was used to provide in-depth knowledge on the metabolic pathways of M. alcaliphilum 20Z grown on methane and methanol. Systems assessment of core carbon metabolism indicated the methanol assimilation pathway is mostly coupled with the efficient Embden-Meyerhof-Parnas (EMP) pathway along with the serine cycle. In addition, an incomplete TCA cycle operated in M. alcaliphilum 20Z on methanol, which might only supply precursors for de novo synthesis but not reducing powers. Instead, it appears that the direct formaldehyde oxidation pathway supply energy for the whole metabolic system. Additionally, a comparative transcriptomic analysis in multiple gammaproteobacterial methanotrophs also revealed the transcriptional responses of central metabolism on carbon substrate change. These findings provided a systems-level understanding of carbon metabolism and new opportunities for strain design to produce relevant products from different C1-feedstocks.

Long-range proton-coupled electron transfer in biological energy conversion: towards mechanistic understanding of respiratory complex I.

Biological energy conversion is driven by efficient enzymes that capture, store and transfer protons and electrons across large distances. Recent advances in structural biology have provided atomic-scale blueprints of these types of remarkable molecular machinery, which together with biochemical, biophysical and computational experiments allow us to derive detailed energy transduction mechanisms for the first time. Here, I present one of the most intricate and least understood types of biological energy conversion machinery, the respiratory complex I, and how its redox-driven proton-pump catalyses charge transfer across approximately 300 Å distances. After discussing the functional elements of complex I, a putative mechanistic model for its action-at-a-distance effect is presented, and functional parallels are drawn to other redox- and light-driven ion pumps.

The dynamic stator stalk of rotary ATPases

Rotary ATPases couple ATP hydrolysis/synthesis with proton translocation across biological membranes and so are central components of the biological energy conversion machinery. Their peripheral stalks are essential components that counteract torque generated by rotation of the central stalk during ATP synthesis or hydrolysis. Here we present a 2.25-Å resolution crystal structure of the peripheral stalk from Thermus thermophilus A-type ATPase/synthase. We identify bending and twisting motions inherent within the structure that accommodate and complement a radial wobbling of the ATPase headgroup as it progresses through its catalytic cycles, while still retaining azimuthal stiffness necessary to counteract rotation of the central stalk. The conformational freedom of the peripheral stalk is dictated by its unusual right-handed coiled-coil architecture, which is in principle conserved across all rotary ATPases. In context of the intact enzyme, the dynamics of the peripheral stalks provides a potential mechanism for cooperativity between distant parts of rotary ATPases.

1,3-Propanediol dehydrogenases in Lactobacillus reuteri: impact on central metabolism and 3-hydroxypropionaldehyde production

BackgroundLactobacillus reuteri metabolizes glycerol to 3-hydroxypropionaldehyde (3-HPA) and further to 1,3-propanediol (1,3-PDO), the latter step catalysed by a propanediol dehydrogenase (PDH). The last step in this pathway regenerates NAD+ and enables therefore the energetically more favourable production of acetate over ethanol during growth on glucose.ResultsA search throughout the genome of L. reuteri DSM 20016 revealed two putative PDHs encoded by ORFs lr_0030 and lr_1734. ORF lr_1734 is situated in the pdu operon encoding the glycerol conversion machinery and therefore likely involved in 1,3-PDO formation. ORF lr_0030 has not been associated with PDH-activity so far. To elucidate the role of these two PDHs, gene deletion mutant strains were constructed. Growth behaviour on glucose was comparable between the wild type and both mutant strains. However, on glucose + glycerol, the exponential growth rate of Δlr_0030 was lower compared to the wild type and the lr_1734 mutant. Furthermore, glycerol addition resulted in decreased ethanol production in the wild type and Δlr_1734, but not in Δlr_0030. PDH activity measurements using 3-HPA as a substrate revealed lower activity of Δlr_0030 extracts from exponential growing cells compared to wild type and Δlr_1734 extracts.During biotechnological 3-HPA production using non-growing cells, the ratio 3-HPA to 1,3-PDO was approximately 7 in the wild type and Δlr_0030, whereas this ratio was 12.5 in the mutant Δlr_1734.ConclusionThe enzyme encoded by lr_0030 plays a pivotal role in 3-HPA conversion in exponential growing L. reuteri cells. The enzyme encoded by lr_1734 is active during 3-HPA production by non-growing cells and this enzyme is a useful target to enhance 3-HPA production and minimize formation of the by-product 1,3-PDO.

Toxoplasma gondiiCyclophilin 18-Mediated Production of Nitric Oxide Induces Bradyzoite Conversion in a CCR5-Dependent Manner

Toxoplasma gondii modulates pro- and anti-inflammatory responses to regulate parasite multiplication and host survival. Pressure from the immune response causes the conversion of tachyzoites into slowly dividing bradyzoites. The regulatory mechanisms involved in this switch are poorly understood. The aim of this study was to investigate the immunomodulatory role of T. gondii cyclophilin 18 (TgCyp18) in macrophages and the consequences of the cellular responses on the conversion machinery. Recombinant TgCyp18 induced the production of nitric oxide (NO), interleukin-12 (IL-12), and tumor necrosis factor alpha through its binding with cysteine-cysteine chemokine receptor 5 (CCR5) and the production of gamma interferon and IL-6 in a CCR5-independent manner. Interestingly, the treatment of macrophages with TgCyp18 resulted in the inhibition of parasite growth and an enhancement of the conversion into bradyzoites via NO in a CCR5-dependent manner. In conclusion, T. gondii possesses sophisticated mechanisms to manipulate host cell responses in a TgCyp18-mediated process.

Can the rat liver maintain normal brain DHA metabolism in the absence of dietary DHA?

Background Docosahexaenoic acid (DHA) is required for normal brain function. The concentration of DHA in the brain depends on both diet and liver metabolism. Objective To determine rat brain DHA concentration and consumption in relation to dietary n-3 (omega-3) polyunsaturated fatty acid (PUFA) content and liver secretion of DHA derived from circulating α-linolenic acid (α-LNA). Design Following weaning, male rats were fed for 15 weeks either: (1) a diet with a high DHA and α-LNA content, (2) an n-3 PUFA “adequate” diet containing 4.6% α-LNA but no DHA, or (3) an n-3 PUFA “deficient” diet containing 0.2% α-LNA and no DHA. Brain DHA consumption rates were measured following intravenous infusion in unanesthetized rats of [1- 14C]DHA, whereas liver and brain DHA synthesis rates were measured by infusing [1- 14C]α-LNA. Results Brain DHA concentrations equaled 17.6, 11.4 and 7.14 μm/g in rats on diets 1, 2 and 3, respectively. With each diet, the rate of brain DHA synthesis from α-LNA was much less than the brain DHA consumption rate, whereas the liver synthesis-secretion rate was 5–10 fold higher. Higher elongase 2 and 5 and desaturase Δ5 and Δ6 activities in liver than in brain accounted for the higher liver DHA synthesis rates. Furthermore, these enzymes were transcriptionally upregulated in liver but not in brain of rats fed the deficient diet. Conclusions While DHA is essential to normal brain function, this need might be covered by dietary α-LNA when liver metabolic conversion machinery is intact and the diet has a high α-LNA content.

Mechanical Design of Translocating Motor Proteins

Translocating motors generate force and move along a biofilament track to achieve diverse functions including gene transcription, translation, intracellular cargo transport, protein degradation, and muscle contraction. Advances in single molecule manipulation experiments, structural biology, and computational analysis are making it possible to consider common mechanical design principles of these diverse families of motors. Here, we propose a mechanical parts list that include track, energy conversion machinery, and moving parts. Energy is supplied not just by burning of a fuel molecule, but there are other sources or sinks of free energy, by binding and release of a fuel or products, or similarly between the motor and the track. Dynamic conformational changes of the motor domain can be regarded as controlling the flow of free energy to and from the surrounding heat reservoir. Multiple motor domains are organized in distinct ways to achieve motility under imposed physical constraints. Transcending amino acid sequence and structure, physically and functionally similar mechanical parts may have evolved as nature's design strategy for these molecular engines.

Lubricant degradation and wear behaviour in a spark-ignition engine

For protecting the environment and increasing convenience in service, the automotive industry is endeavouring to optimise the oil-change intervals. On behalf of the German Research Association for Combustion Engines (FVV), three research partners have investigated the degradation of lubricants and its effect on the wear behaviour of the tribo-systems in a spark-ignition engine in the course of a joint research project. The partners are the Institute of Tribology and Energy Conversion Machinery at Clausthal University of Technology, the Institute of Machine Elements and Design Engineering at University of Technology Kassel, and the Institute of Surface and Coating Analysis at University of Technology Kaiserslautern.

Genetic manipulation of an exogenous non-immunoglobulin protein by gene conversion machinery in a chicken B cell line

During culture, a chicken B cell line DT40 spontaneously mutates immunoglobulin (Ig) genes by gene conversion, which involves activation-induced cytidine deaminase (AID)-dependent homologous recombination of the variable (V) region gene with upstream pseudo-V genes. To explore whether this mutation mechanism can target exogenous non-Ig genes, we generated DT40 lines that bears a gene conversion substrate comprising the green fluorescent protein (GFP) gene as a donor and the blue fluorescent protein (BFP) gene as an acceptor. A few percent of the initially BFP-expressing cells converted their fluorescence from blue to green after culture for 2–3 weeks when the substrate construct was integrated in the Ig light chain locus, but not in the ovalbumin locus. This was the result of AID-dependent and the GFP gene-templated gene conversion of the BFP gene, thereby leading to the introduction of various sizes of GFP-derived gene segment into the BFP gene. Thus, G/B construct may be used to visualize gene conversion events. After switching off AID expression in DT40 cells, the mutant clones were isolated stably and maintained with their mutations being fixed. Thus, the gene conversion machinery in DT40 cells will be a useful means to engineer non-Ig proteins by a type of DNA shuffling.

Secondary sulphonylurea failure: what pathogenesis is responsible?

Sulphonylurea (SU) stimulates insulin secretion by pancreatic β-cells and is generally used as a first-line treatment for type 2 diabetes. However, after long-term SU treatment (six months or over), some patients begin to show an increase in blood glucose once again (secondary SU failure). Two theories have been put forward to explain this failure – dysfunction of the proinsulin conversion machinery or insulin resistance. However, the primary pathogenesis behind secondary SU failure still needs to be investigated. Using a reliable technique that specifically identifies intact proinsulin (IPI), total proinsulin (TPI) and specific insulin (SI), this study aims to discover if a defect in the proinsulin converting mechanism plays a role in SU failure. Three groups were recruited for this study: healthy controls (n=8), SU responders (n=38) and secondary SU failures (n= 46). Serum concentrations of insulin-related molecules released in response to a standard glucose challenge test were compared between the groups. It was found that total SI was lower in the patient groups (P<0.05 compared to the control group), while TPI and IPI showed no distinct difference between the three groups (P>0.05). TPI:SI ratio and IPI:SI ratio showed marked increases in the patient groups (P<0.05 compared to control group), with no obvious quantitative difference between SU responders and secondary SU failures (P>0.05). Similar results for the Homa Insulin Resistant Index were found between the two patient groups. Interestingly, blood glucose at 180 mins after glucose challenge was significantly higher in the secondary SU failure group (P<0.05), with no correlation to SI, while the SU responder group showed good correlation between the parameters (P<0.05). We conclude that type 2 diabetes is associated with obvious dysfunction in the proinsulin-converting process and shows severe SI deficiency in responding to glucose challenge. Dysfunction of the proinsulin conversion mechanism was not an extra cause responsible for SU failure.

Structure at 2.3 Å resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment

Background: The cytochrome bc 1 complex is part of the energy conversion machinery of the respiratory and photosynthetic electron transfer chains. This integral membrane protein complex catalyzes electron transfer from ubiquinol to cytochrome c. It couples the electron transfer to the electrogenic translocation of protons across the membrane via a so-called Q cycle mechanism. Results: The cytochrome bc 1 complex from the yeast Saccharomyces cerevisiae was crystallized together with a bound antibody Fv fragment. The structure was determined at 2.3 Å resolution using multiple isomorphous replacement, and refined to a crystallographic R factor of 22.2% (R free = 25.4%). The complex is present as a homodimer. Each ‘monomer’ of the refined model includes 2178 amino acid residues of subunits COR1, QCR2, COB, CYT1, RIP1, QCR6, QCR7, QCR8 and QCR9 of the cytochrome bc 1 complex and of the polypeptides V H and V L of the Fv fragment, the cofactors heme b H, heme b L, heme c 1, the [2Fe–2S] cluster and 346 water molecules. The Fv fragment binds to the extrinsic domain of the [2Fe–2S] Rieske protein and is essential for formation of the crystal lattice. Conclusions: The approach to crystallize membrane proteins as complexes with specific antibody fragments appears to be of general importance. The structure of the yeast cytochrome bc 1 complex reveals in detail the binding sites of the natural substrate coenzyme Q6 and the inhibitor stigmatellin. Buried water molecules close to the binding sites suggest possible pathways for proton uptake and release. A comparison with other cytochrome bc 1 complexes shows features that are specific to yeast.

Processing of proinsulin by transfected hepatoma (FAO) cells.

Rat hepatoma (FAO) cells were stably transfected with the gene encoding either rat proinsulin II (using the DOL retroviral vector) or human proinsulin (using the RSV retroviral vector). Using the DOL vector, production of insulin immunoreactive material was stimulated up to 30-fold by dexamethasone (5 x 10(-7) M). For both proinsulins, fractional release of immunoreactive material relative to cellular content was high, in keeping with the absence of any storage compartment for secretory proteins in these cells. Pulse-chase experiments showed kinetics of release of newly synthesized products in keeping with release via the constitutive pathway. High performance liquid chromatography analysis showed immunoreactivity in the medium distributed between three peaks. For rat proinsulin II, the first coeluted with intact proinsulin; the second coeluted with des-64,65 split proinsulin (the product of endoproteolytic attack between the insulin A-chain and C-peptide followed by trimming of C-terminal basic residues by carboxypeptidase); the third (and minor peak) coeluted with native (fully processed) insulin. For human proinsulin, by contrast, the second peak coeluted with des-31,32 split proinsulin (split and trimmed at the B-chain/C-peptide junction). Analysis of cellular extracts showed intact proinsulin as the major product. The generation of the putative conversion intermediates and insulin was not due to proteolysis of proinsulin after its release but rather to an intracellular event. The data suggest that proinsulin, normally processed in secretory granules and released via the regulated pathway, may also be processed, albeit less efficiently, by the constitutive pathway conversion machinery. The comparison of the sites preferentially cleaved in rat II or human proinsulin suggests cleavage by endoprotease(s) with a preference for R/KXR/KR as substrate.

III. Competition and Co-existence: Indo-Islamic Interaction in Medieval North India

The study of Islam and Muslims in relation to local non-Muslim population and their religious beliefs and social practices in medieval India has often tended to be conducted eventually along two lines, seemingly opposed to each other. On the one hand, there are communal historians who have reduced the history of medieval India into the conflict between Hindus and Muslims, which they have projected as having resulted from their divergent religious outlooks. The period was Islamic in their view, and the state a conversion machinery and an organ to bring Hindus under the hegemony of Islam. This was a mission in which the state could not succeed fully, largely because of ‘Hindu’ resistance. On the other hand, there are a large number of ‘liberal’ historians to whom the hallmark of medieval Indian society has been an amity between the two communities, the various tensions and encounters over economic and political matters notwithstanding. The medieval period, in the opinion of such historians, saw the evolution and efflorescence of a composite culture to which medieval rulers, nobles, sufis and Persian and Urdu poets contributed significantly. The later animosity between Hindus and Muslims and clashes over religious matters, they argued, were the handiwork of the British.

Economic, political and psychological barriers to building the new energy conversion machinery

The importance of the short time scale for the introduction of new energy technologies is indicated and the possible effects outlined. the barriers to changes in philosophy on energy matters are discussed and some approaches are suggested.