Conventional Production Methods Research Articles

Computation-Driven Rational Design of Self-Assembled Short Peptides for Catalytic Hydrogen Production.

Self-assembling peptides represent a captivating area of study in nanotechnology and biomaterials. This interest is largely driven by their unique properties and the vast application potential across various fields such as catalytic functions. However, design complexities, including high-dimensional sequence space and structural diversity, pose significant challenges in the study of such systems. In this work, we explored the possibility of self-assembled peptides to catalyze the hydrolysis of hydrosilane for hydrogen production using ab initio calculations and carried out wet-lab experiments to confirm the feasibility of these catalytic reactions under ambient conditions. Further, we delved into the nuanced interplay between sequence, structural conformation, and catalytic activity by combining modeling with experimental techniques such as transmission electron microscopy and nuclear magnetic resonance and proposed a dual mode of the microstructure of the catalytic center. Our results reveal that although research in this area is still at an early stage, the development of self-assembled peptide catalysts for hydrogen production has the potential to provide a more sustainable and efficient alternative to conventional hydrogen production methods. In addition, this work also demonstrates that a computation-driven rational design supplemented by experimental validation is an effective protocol for conducting research on functional self-assembled peptides.

Metabolic Engineering of Corynebacterium glutamicum for the Production of Flavonoids and Stilbenoids

Flavonoids and stilbenoids, crucial secondary metabolites abundant in plants and fungi, display diverse biological and pharmaceutical activities, including potent antioxidant, anti-inflammatory, and antimicrobial effects. However, conventional production methods, such as chemical synthesis and plant extraction, face challenges in sustainability and yield. Hence, there is a notable shift towards biological production using microorganisms like Escherichia coli and yeast. Yet, the drawbacks of using E. coli and yeast as hosts for these compounds persist. For instance, yeast’s complex glycosylation profile can lead to intricate protein production scenarios, including hyperglycosylation issues. Consequently, Corynebacterium glutamicum emerges as a promising alternative, given its adaptability and recent advances in metabolic engineering. Although extensively used in biotechnological applications, the potential production of flavonoid and stilbenoid in engineered C. glutamicum remains largely untapped compared to E. coli. This review explores the potential of metabolic engineering in C. glutamicum for biosynthesis, highlighting its versatility as a cell factory and assessing optimization strategies for these pathways. Additionally, various metabolic engineering methods, including genomic editing and biosensors, and cofactor regeneration are evaluated, with a focus on C. glutamicum. Through comprehensive discussion, the review offers insights into future perspectives in production, aiding researchers and industry professionals in the field.

Waste CO2 capture and utilization for methanol production via a novel membrane contactor reactor process: techno-economic analysis (TEA), and comparison with other existing and emerging technologies

We introduced recently a CO2 capture and utilization (CCU) technology that produces methanol (MeOH), from waste CO2 employing a novel configuration combining a reverse water gas shift reactor (RWGSR) with a membrane contactor reactor for MeOH synthesis (MeS-MCR). The integrated RWGSR/MeS-MCR process overcomes the challenges direct CO2 conversion into MeOH faces and achieves carbon conversions exceeding 80%, significantly surpassing the equilibrium conversion of ∼ 20 to 30 % for the MeS reaction. In this study, a techno-economic analysis (TEA) was conducted on the RWGSR/MeS-MCR process along with four other CO2-to-MeOH CCU processes. Key economic parameters, as well as energy efficiency, were analyzed with respect to CO2 and renewable hydrogen (H2) costs and plant capacity. The study revealed that the RWGSR/MeS-MCR technology has the highest energy efficiency and the best economic performance among all the technologies investigated. All technologies offer the significant societal benefit of capturing and utilizing waste CO2, but under present CO2 and renewable H2 costs, they cannot currently compete cost-wise with conventional MeOH production methods. However, under a future scenario whereby government incentives lower the CO2 cost and technological advances decrease the price of renewable H2, the proposed RWGSR/MeS-MCR and another catalytic technology (CAMERE) become price competitive.

Nanotechnology-based biodiesel: A comprehensive review on production, and utilization in diesel engine as a substitute of diesel fuel

As a sustainable replacement for fossil fuels, biodiesel is a game-changer in the energy sector. There is no strategy to minimize biodiesel's significance as a sustainable, clean fuel source in light of the increasing climate change and environmental sustainability concerns. Nevertheless, conventional biodiesel production methods often run into problems like inadequate conversion efficiency and inappropriate fuel properties, which impede their broad adoption. The revolutionary potential of nanotechnology to circumvent these limitations and revolutionize biodiesel consumption and production is explored in this review paper. There are new possibilities for improving biodiesel output and engine efficiency, thanks to nanotechnology, which can alter matter at the atomic and molecular levels. Using nano-catalysts, nano-emulsification processes, and nano-encapsulation procedures, researchers have made significant advances in improving biodiesel qualities such as stability, combustion efficiency, and viscosity. Through a comprehensive analysis of current literature and research data, this article elucidates the crucial role of nanotechnology in advancing biodiesel technology. By shedding light on the most current advancements, challenges, and potential future outcomes in nano-based biodiesel manufacturing and consumption, this review hopes to add to the growing corpus of knowledge in the field and inspire additional innovation. In conclusion, there is great hope for a sustainable energy future, increased economic growth, and reduced environmental impacts through the application of nanotechnology.

Experimental Investigation on Effect of Shell Thickness and Infill Density on Mechanical Properties of Polymer 3D Printing Process (MEX)

Abstract: A cutting-edge advancement in advanced manufacturing is the use of a revolutionary technique called threedimensional printing. The primary objective of three-dimensional printing, in comparison to traditional manufacturing methods, is to efficiently create intricate geometries in a shorter timeframe while maintaining the desired characteristics of the object. This task cannot be achieved by conventional production methods. In order to produce any component, it is vital for us to get the necessary material and then secure the power supply. In the process of three-dimensional printing, a diverse range of materials, such as polymers, metal powders, ceramic powder, and others, are used. Several variables contribute to the situation, including the amount of material used. In this specific instance, we are striving to maximize the use of material while maintaining the product's quality prior to printing. The criteria that have the most significant influence on the quantity of material required and the printing time are mostly determined by several key features. This category encompasses several factors, like layer height, infill density, print speed, shell thickness, and more. Furthermore, the line width or layer width is an additional criterion that significantly affects the amount of material used. Expanding the line width will lead to a higher expenditure of material, while reducing the layer width will result in a reduction in material consumption. The breadth of a layer is directly proportional to the amount of material used. The width of this line directly affects the duration of the printing process, the strength of the bond, and the quality of the completed product's surface. Significant changes in the line width could potentially lead to a print failure. Our objective is to enhance the efficiency of material use and printing time by adjusting the line width while maintaining the same layer height and applying various infill densities. This will allow us to maintain a consistent layer height. This research unequivocally proves that a model with 100% infill has no impact on the amount of material required. However, it does result in an increased printing time as the shell thickness decreases, printing time increases. The discoveries made in this study clearly demonstrate this. However, the layer width exhibits varying behavior depending on the kind of infill utilized. Decreasing the degree of infill not only reduces the amount of material used, but also increases the printing time.

Corrosion inhibition of mild steel by Praecitrullus fistulosus (tinda fruit and peel) extracts

Metal corrosion has recently emerged as a growing concern, impacting both local and industrial operations and disrupting conventional production methods. The utilization of green inhibitors to mitigate the metal degradation has garnered extensive attention from researchers and industrial professionals due to their prominent advantages: high efficiency, cost-effectiveness, and eco-friendliness. A novel ecofriendly inhibitor was prepared from Praecitrullus fistulosus (tinda fruit and peel) for mild steel (MS) corrosion in 1 M HCl. The presence of phenol, 3,5-bis (1,1-dimethyl ethyl)-, 9-octadecenoic acid, methyl ester, hexadecanoic acid 15-methyl-, methyl ester, 9, 12-octadecadienoic acid, methyl ester, 9, 12, 15-octadecatrienoic acid, methyl ester, (Z,Z,Z-), ascorbic acid, and phytol were identified as major constituent through LC/MS analysis of tinda extracts. The existence of these compounds was further confirmed through FTIR analysis, which shows the presence of various functional groups, such as -OH, CO, C-O-C, CC, and aromatic rings in the tinda extracts. Electrochemical and gravimetric analyses were used to investigate the inhibitory effect of tinda extracts. Outcomes of Tafel analysis revealed that both tinda extracts significantly reduced the corrosion current as compared to blank and achieved 83.73 % and 87.59 % inhibition efficiencies at 200 mg L−1 of tinda peel extract (TPE) and tinda fruit extract (TFE), respectively. The change in corrosion potential (Ecorr) was within an ±85 mV range compared to that of the uninhibited system, indicating that both tinda extracts demonstrated a mixed-type inhibition behavior. During adsorption studies, the best fit was obtained for the Langmuir adsorption model. The obtained values of standard Gibbs free energy (ΔG°ads) for TPE and TFE lie between −20 and − 40 kJ mol−1 but close to −20 kJ mol−1, which reveals preferential physical adsorption of the extracts on the metal surface. Thermodynamic parameters, including activation energy, enthalpy, and entropy, were computed across the temperature range of 303 to 323 K, suggesting that corrosion occurs spontaneously by the endothermic process. FESEM analysis depicted that inhibited systems exhibited smooth and crack-free surfaces as compared to blank system. AFM images demonstrated that surface roughness was significantly reduced for the inhibited system. In EDX analysis, the weight percentage of Cl was reduced in the presence of tinda extracts as compared to blank, and in XRD analysis, iron chloride (FeCl2) peak did not appear in the presence of inhibitor but it was in the uninhibited system. All surface-related findings signify that tinda extracts are adsorbed on the MS surface and form a protective layer that separates the metal from the corrosive solution.

Towards greener approach: Techno-economic insights into formaldehyde bio production from a hybrid pine and mustard biomass combination

In an era marked by environmental crises and dwindling fossil resources, the need to revolutionize industrial processes for sustainability is paramount. Formaldehyde synthesis, a pivotal precursor in industries spanning polymers, resins, and textiles, stands at a critical juncture where innovation and necessity intersect. Conventional methods of formaldehyde production, despite their efficiency, remain tethered to non-renewable feedstocks and energy-intensive techniques, perpetuating ecological harm and resource constraints. This study explores eco-friendly pathways for formaldehyde production. Recent strides in bio-based chemistry enable the creation of formaldehyde from renewable biomass, reducing energy use and carbon emissions. Decentralized production with locally sourced biomass boosts supply chain resilience and community-centered sustainability. The research employs Aspen Plus® software to model a two-step process: gasification of a hybrid pine and mustard biomass blend to produce bio-methanol, followed by its oxidation into formaldehyde. Sensitivity analyses demonstrate temperature's impact on syngas composition, with higher temperatures favoring hydrogen production. The air-to-biomass ratio affects carbon dioxide content and energy content, crucial for efficient energy production. In formaldehyde synthesis, the air-to-methanol ratio significantly influences yield. Economically, formaldehyde bio- production shows promise, with an 8.50% return on investment and a 22.16-year payback period. An annual net profit of $2.41 million is projected, with a break-even point at $53.47 million in total sales revenue. This research underscores the convergence of sustainability, innovation, and economic viability in formaldehyde bio-production, offering valuable insights for environmentally friendly chemical manufacturing and the broader transition to ecological progress.

Comparative study of a two-step enzymatic process and conventional chemical methods for biodiesel production: Economic and environmental perspectives

The urgent need to address greenhouse gas (GHG) emissions underscores the significance of biodiesel as a key liquid biofuel. Compared to chemical biodiesel production, enzymatic technology offers a more sustainable alternative. This article presents a comparative study between a two-step enzymatic biodiesel production technology, already successfully industrialized, and conventional chemical technologies utilizing soybean oil (SBO) and waste cooking oil (WCO) as feedstock, respectively. The economic analysis demonstrates significant cost savings with the enzymatic process resulting in reductions of 16.33% and 36.54% for SBO and WCO, respectively. Enzymatic technology also exhibits substantial reductions in energy consumption, with a decrease of 86.8% and 60.2% for SBO and WCO, respectively. LCA findings indicate that enzymatic technology diminishes environmental impacts and GHG emissions are less than 78.86% and 63.05% for SBO and WCO, respectively. This study provides crucial insights for decision-makers, marking a significant paradigm shift in biodiesel production.

Efficient preparation of icariin from epimedin C by recyclable biphasic enzymatic hydrolysis

Icariin is the most bioactive ingredient of Epimedium L. and a quality marker of Herba Epimedii. Conventional methods for production of Icariin are known to be inefficient, resulting in low yields and significant environmental pollution. This study aimed to develop a sustainable and effective biphasic enzymatic hydrolysis system for the efficient conversion of epimedin C to icariin. The biphasic system was created using butyl acetate and phosphate buffer (pH 4.5) at a ratio of 3:1 (V/V) along with α-L-rhamnosidase/epimedin C (2 U/1 mg) at 50 °C for 12 h. Consequently, 98.21% of epimedin C was hydrolysed to icariin, with 95.62% of the product being transferred to the organic phase. Even after four cycles of use, the conversion ratio remained high at 75.28%. Furthermore, this novel strategy was also used for the conversion of Epimedium brevicornu Maxim. extracts. The biphasic system represents a sustainable and effective method for icariin production, offering potential benefits for industrial applications.

Estimating Impact of Conventional and System of Rice Intensification (SRI) Methods of Rice Production Management on Sustainable Livelihood Generation in the Context of Climate Change in Maharashtra, India: A Factor Analysis

The global agricultural community has been preparing to face the imminent threat posed by climate change as projections by scientific communities complement the fact that food production will be affected adversely in the near future. The Indian subcontinent has not been explicit about the effects of natural disasters like floods and cyclones caused due to climatic changes. The aberrant weather patterns along with the inconsistent output of monsoon create unprecedented challenges for the rice-dominated farming system to thrive. It directly impacts the livelihood of the farmers making them economically vulnerable. Thus, it has become necessary for the research community to explore different causal dimensions related to rice production and suggest remedies for the constantly changing agroecosystem. This study aimed to investigate different indicators of impact on sustainable livelihood generation through rice production management in light of climate change in both conventional as well as SRI methods of rice production. An ex post facto research design was adopted for conducting the study. Purposive sampling was used to select the state, district, and talukas keeping in view the backwardness and agricultural status of the area of the study. Using a simple random sampling method, two hundred (200) respondents were selected from two talukas of the Bhandara district of Maharashtra. In case of results found in conventional method of rice production, the variables, Water management, Farm size, Education, Irrigation index%, Selling% and Risk orientation have contributed 9.996 per cent of variance and clubbed into Factor 1. On the other hand, in the case of SRI method, Factor 1 has the following variables i.e., Farm size, Family income primary, Education, Primary occupation, Mass media exposure, Family education status and Sowing time which has contributed 9.54 per cent of variance and has been renamed as ‘Family capability’.

Bipolar Membrane Seawater Splitting for Hydrogen Production: A Review.

The growing demand for clean energy has spurred the quest for sustainable alternatives to fossil fuels. Hydrogen has emerged as a promising candidate with its exceptional heating value and zero emissions upon combustion. However, conventional hydrogen production methods contribute to CO2 emissions, necessitating environmentally friendly alternatives. With its vast potential, seawater has garnered attention as a valuable resource for hydrogen production, especially in arid coastal regions with surplus renewable energy. Direct seawater electrolysis presents a viable option, although it faces challenges such as corrosion, competing reactions, and the presence of various impurities. To enhance the seawater electrolysis efficiency and overcome these challenges, researchers have turned to bipolar membranes (BPMs). These membranes create two distinct pH environments and selectively facilitate water dissociation by allowing the passage of protons and hydroxide ions, while acting as a barrier to cations and anions. Moreover, the presence of catalysts at the BPM junction or interface can further accelerate water dissociation. Alongside the thermodynamic potential, the efficiency of the system is significantly influenced by the water dissociation potential of BPMs. By exploiting these unique properties, BPMs offer a promising solution to improve the overall efficiency of seawater electrolysis processes. This paper reviews BPM electrolysis, including the water dissociation mechanism, recent advancements in BPM synthesis, and the challenges encountered in seawater electrolysis. Furthermore, it explores promising strategies to optimize the water dissociation reaction in BPMs, paving the way for sustainable hydrogen production from seawater.

Outdoor biohydrogen production by thermotolerant Rhodopseudomonas pentothenatexigens KKU-SN1/1 in a cluster of ten bioreactors system.

In tropical regions, the viability of outdoor photo-fermentative biohydrogen production faces challenges arising from elevated temperatures and varying light intensity. This research aimed to explore how high temperatures and outdoor environments impact both biohydrogen production and the growth of purple non-sulfur bacteria. Our findings revealed the potential of Rhodopseudomonas spp. as a robust outdoor hydrogen-producing bacteria, demonstrating its capacity to thrive and generate biohydrogen even at 40°C and under fluctuating outdoor conditions. Rhodopseudomonas harwoodiae NM3/1-2 produced the highest cumulative biohydrogen of 223mL/L under anaerobic light conditions at 40°C, while Rhodopseudomonas harwoodiae 2M had the highest dry cell weight of 2.93g/L. However, R. harwoodiae NM3/1-2 demonstrated the highest dry cell weight of 3.99g/L and Rhodopseudomonas pentothenatexigens KKU-SN1/1 exhibited the highest cumulative biohydrogen production of 400mL/L when grown outdoors. In addition, the outdoor enhancement of biohydrogen production was achieved through the utilization of a cluster of ten bioreactors system. The outcomes demonstrated a notable improvement in biohydrogen production efficiency, marked by the highest daily biohydrogen production of 493mL/Ld by R. pentothenatexigens KKU-SN1/1. Significantly, the highest biohydrogen production rate was noted to be 17 times greater than that observed in conventional batch production methods. This study is the first to utilize R. pentothenatexigens and R. harwoodiae for sustained biohydrogen production at high temperatures and in outdoor conditions over an extended operational period. The successful utilization of a clustered system of ten bioreactors demonstrates potential to scale-up for industrial biohydrogen production.

Optimizing media and substrate for efficient anaerobic biological ammonia synthesis: A response surface methodology approach

Ammonia ranks among the most extensively produced natural and anthropogenic chemicals due to its immense applications, especially in agriculture as fertilizer. The global demand for ammonia has thus been projected to increase. Haber process, the conventional method of ammonia production is costly, energy intensive and responsible for ∼2% of CO2 emission. The rumen Hyper Ammonia Producing Bacteria (HAB) has the potential to convert protein to ammonia. To develop an industrial feasible ammonia production alternative, this study aims to optimize soy-based media composition for biological ammonia using response surface methodology (RSM). A one-factor-at-a-time (OFAT) statistical design was used to determine the most important media components affecting biological ammonia production. Eight media components were screened, and results showed that K2HPO4, KH2PO4, Na2SO4 and soy meal protein isolate (SMPI) had the most significant impact on biological ammonia production. The four identified media components were further optimized using a central composite design (CCD). The RSM model equation for independent and response variables was computed and used to create the response surface and contour plots. Results showed a maximum biological ammonia experimental yield of ∼7,500 mg/L at 0.0492 g/100 mL K2HPO4, 0.0492 g/100 mL KH2PO4, 0.64 g/100 mL Na2SO4, and 10 % SMPI. The observed yield was ∼80 % higher than the biological ammonia production from previous studies.

Mid-Long Chain Dicarboxylic Acid Production via Systems Metabolic Engineering: Progress and Prospects.

Mid-to-long-chain dicarboxylic acids (DCAi, i ≥ 6) are organic compounds in which two carboxylic acid functional groups are present at the terminal position of the carbon chain. These acids find important applications as structural components and intermediates across various industrial sectors, including organic compound synthesis, food production, pharmaceutical development, and agricultural manufacturing. However, conventional petroleum-based DCA production methods cause environmental pollution, making sustainable development challenging. Hence, the demand for eco-friendly processes and renewable raw materials for DCA production is rising. Owing to advances in systems metabolic engineering, new tools from systems biology, synthetic biology, and evolutionary engineering can now be used for the sustainable production of energy-dense biofuels. Here, we explore systems metabolic engineering strategies for DCA synthesis in various chassis via the conversion of different raw materials into mid-to-long-chain DCAs. Subsequently, we discuss the future challenges in this field and propose synthetic biology approaches for the efficient production and successful commercialization of these acids.

Green synthesis of bioactive nanocomposites using Diploschistes scruposus lichen and investigation of cytotoxic effects on cancer cells

Green synthesis of nanoparticles, which is a safer and more environmentally friendly approach than traditional nanoparticle production methods, emerges as a promising field of the future. The green synthesis method, utilizing lichen extracts, offers a sustainable alternative to conventional nanoparticle production methods. Lichens have the ability to reduce and stabilize metallic salts, in addition to possessing medicinal effects from the secondary compounds they contain. In this context, the properties of lichens pave the way for a promising avenue in which nanotechnology can be harnessed within the field of cancer therapy. Building upon this back-ground, Diploschistes scruposus was employed in the production of nanoparticles through the green synthesis method. The study conducted the synthesis of lichen-derived silver-zinc oxide, silver-strontium oxide, and silver-nickel oxide nanoparticles, leveraging the characteristics of the Diploschistes scruposus species. It is specifically highlighted that toxicity studies concerning strontium oxide and nickel oxide nanoparticles are lacking. This aspect renders the study a valuable resource for addressing a significant knowledge gap in these fields and comprehending the potential biomedical applications of these nanoparticles. The properties of the resulting nanoparticles were determined through analysis of characteristic FE-SEM for morphological examination, and FT-IR was utilized to identify the presence of functional compo-nents. EDX methods were employed for elemental analysis, and XRD methods were used for crystal structure analysis. The hydrodynamic diameters of the nanoparticles were determined by DLS (Dynamic Light Scattering). In addition to characterization, the materials were tested for cytotoxi-city in breast cancer cells (MCF-7 and MDA-MB-231) using MTT test. According to the MTT assay, toxicity studies were dose-dependent and the lowest IC50 value was found in the MCF-7 cell line with Ag–ZnO at 4.25 ± 0.014 μg/mL.

Sustainable protein production through genetic engineering of cyanobacteria and use of atmospheric N2 gas

AbstractThis review explores the potential of genetically engineering cyanobacteria with the aim of synthesizing high‐value protein directly from atmospheric nitrogen. The article examines numerous techniques that may enhance protein synthesis in cyanobacteria, and discusses advantages, barriers, and opportunities for this strategy going forward. Genetic manipulation of cyanobacteria shows promise in sustainably raising protein production via reduced greenhouse gas emissions and lower dependence on synthetic fertilizers, but also potentially fewer environmental implications traditionally caused by conventional protein production methods. The article uncovers many difficulties in genetically modifying cyanobacteria for protein production. For example, genetically modified organisms (GMOs) have legal and regulatory ramifications that must be accounted for if ethical, moral and secure use of these technologies is to be ensured. Economic viability, too, must be evaluated, taking into consideration production costs, scalability, market demand and future market potential. We suggest that processing of cyanobacterial proteins in downstream stages need further development. Effective and economical methods are needed for protein extraction, purification, and formulation into commercially viable products. For successful application of cyanobacterial protein production at scale, such obstacles must be overcome. We conclude that genetic engineering of cyanobacteria for protein synthesis has a great deal of potential to offer a resource‐effective and sustainable replacement for the synthesis of high‐value proteins, so promoting a more sustainable and environmentally conscious future.

Determination of the Effect of Poultry Ash Application on Agro Morphological and Quality Properties of Potato (Solanum tuberosum L.)

This study was carried out in Bolu conditions in 2021 to see the effect of poultry ash, which has the potential to be a nutrient in terms of being rich in minerals and is also a soil conditioner, on the yield and quality characteristics of potato (Solanum tuberosum L.). In the study, the conventional production method of 8 kg/da P + 20 kg/da N application was tried with six different ash amounts (0, 500, 1000, 1500, 2000 kg/da poultry ash + 20 kg/da N). According to the values obtained from the study, the highest plant height (cm), the number of main stems (pieces), the specific gravity (g) 1500 kg/da ash application, the number of tubers per plant (pieces) from farmer conditions; average tuber weight (g), french fries oil rate (%), protein rate (%), big tuber rate (%) 2000 kg/da; chips oil rate (%), small tuber rate (%), dry matter rate (%) in the control plot where there was no application; Tuber yield per decare (kg/da), medium tuber ratio (%), marketable tuber ratio (%), were obtained from 1000 kg/da poultry ash application.

Individual self-regulation, external monitoring, and farmers’ safe production behavior: Evidence from the Kuan-chung Plain, China

Sustainable agricultural development requires comprehending the fundamental factors influencing farmers' adoption of safe production behavior. This study investigates intrinsic and extrinsic determinants, encompassing the influence of individual self-regulation and external monitoring, regarding the endorsement of safe labor practices among farmers in Kuan-Chung Plain, China. The findings underscore the pivotal role of personal self-regulation in stimulating farmers' implementation of safe production behavior. Additionally, governmental and public monitoring can act as catalysts, motivating farmers to shift from conventional agricultural production methods to safer alternatives. Moreover, the results revealed that a synergistic effect arises from the collaboration between public and governmental monitoring, combined with individual self-regulation. This collaborative approach significantly enhances farmers' propensity to embrace safe production behavior. Hence, policymakers should prioritize educating farmers on ethical restraint, optimizing policy strategies, and strengthening supervision practices to establish an effective platform for public monitoring. These measures will augment farmers' comprehension of the significance of safe production behavior and empower them to proactively implement these behaviors.

On the formation of dendritic iron from alkaline electrochemical reduction of iron oxide prepared for metal fuel applications

Low-temperature electrochemical reduction (electroreduction) of iron oxides is a promising alternative to the conventional methods for iron production due to its CO2-free operation and relatively low energy consumption. In this work, we demonstrate a novel approach for electrochemical iron production by promoting the formation of dendritic structures during iron electrodeposition, which facilitates the easy harvesting of deposits in powder form. Experiments were conducted using a single pair of parallel plate electrodes, immersed in a mixture of hematite (Fe2O3) powder and aqueous alkaline (NaOH) slurry. The effects of current density, Fe2O3 mass fraction, temperature, and powder size on current efficiency and deposit morphology are investigated. A large quantity of dendritic iron structures is observed when experiments are carried out without stirring and/or applying heat from a heating plate. This condition suggests temperature and (ion/species) concentration gradients in the system. The dendrites are mainly deposited on the cathode's sides, corners, and edges. Different deposits and dendritic structures (compact layer deposit, moss-like deposit, deposit with whisker-like dendrites, and deposit with crystal-like dendrites) are observed as operating conditions change. Overall, a cathodic deposition of metallic iron with a high Faradaic efficiency (≥90 %) is successfully accomplished. The present findings provide new insights into the production of electrolytic iron powder and its future use as a carbon neutral and sustainable fuel/energy carrier.

Human-powered hydroponic systems: An environmental and economic assessment

Hydroponic systems like vertical farming have been proposed as an alternative and potentially more sustainable option for growing vegetables than conventional soil-based production methods. However, the energy required for the functioning of hydroponic systems is a key contributor to the total environmental impact and economic cost. Human-powered hydroponic systems, which utilise human muscular exertion to power the system, have not been thoroughly investigated, despite their potential as a cost-efficient alternative and less harmful to the environment. This paper presents a prototype of a novel human-powered hydroponic system and assesses its environmental impact and economic costs using life cycle assessment (LCA) and life cycle costing (LCC), considering the cultivation of lettuce as a case study. A scenario analysis was performed to assess the environmental impact of lettuce production using a human-powered hydroponic system compared to conventional soil or greenhouse-based production methods. Using SimaPro LCA software, 18 impact categories included in the ReciPe Midpoint (H) method were evaluated. The results indicated that the battery, followed by the power system, represented the primary environmental hotspots of the human-powered hydroponic system. Improving the yield (production of 6 lettuces of 500 g vs 200 g per 40-day growing cycle) of the human-powered hydroponic system can reduce the environmental impact of lettuce production compared to conventionally soil-based produced lettuce in 11 out of the 18 evaluated impact categories, and when compared to greenhouse-produced lettuce, it generates lower impacts in 14 out of 18 categories. Regarding economic cost, considering a yield of 1.2 kg (6 lettuces of 200 g) per 40-day growing cycle with the human-powered hydroponic system, it is more expensive for the consumer than buying lettuce produced with conventional soil-based open-field agriculture and greenhouses, and break-even points are not achievable within 15 years (the lifespan of the system). However, when considering a yield of 3 kg (6 lettuces of 500 g), the break-even point with greenhouse-produced lettuce was achieved between 10 and 11 years. Therefore, although a break-even point is not achievable within the considered lifespan of the developed system, it could be reached if the yield of the system increased and/or if the system had a longer lifespan, which is mainly determined by the lifespan of the battery.