Event Abstract Back to Event Production of lacZ inducible T cell hybridoma specific for human and mouse gp10025-33 peptides Gal Cafri1, Adi Sharbi-Yunger1, Esther Tzehoval1, Zoya Alteber1, Meir Azulay1, Yosi M. Gozlan1 and Lea Eisenbach1* 1 Weizmann Institute of Science, Israel The key event in T cell activation is the recognition of a peptide bound to major histocompatibility complex (MHC) molecules on the surface of antigen presenting cells (APCs). The enormous pool of peptides displayed on MHC makes it almost impossible to detect a given peptide-MHC complex on the surface of APCs by using conventional indirect methods. On the other hand, direct recognition of a selected peptide by the TCR results in generation of intracellular signals leading to initiation of the primary stages of T cell activation. To facilitate measurement of T cell activation and to enable identification of individual clones, β-galactosidase (lacZ) inducible CD4+ and CD8+ T cell hybrids were developed. Previous studies showed that heterologous Escherichia coli β-galactosidase (lacZ) gene, under control of the IL-2 entire enhancer region or the nuclear factor in activated T cells (NFAT) element alone, is specifically induced in transfected and activated T cells. Thus, activation of transfected T cells, results in synthesis of both IL-2 and lacZ gene products. Moreover, since the lacZ remains sequestered within the activated cells, chromogenic or fluorogenic substrate enables measurement of an activating event in a single T cell. Generation of the hybrids is relatively easy and allows maintenance in culture and the lacZ assay provides a rapid, sensitive and non-radioactive method for measuring T cell activation. In this study we isolated T cells from Pmel-1 mice and generated a lacZ inducible CD8+ T cell hybridoma. Keywords: hybridoma, tcr, gp100 Melanoma Antigen, Antigen Presentation, activation Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: Cafri G, Sharbi-Yunger A, Tzehoval E, Alteber Z, Azulay M, Gozlan YM and Eisenbach L (2013). Production of lacZ inducible T cell hybridoma specific for human and mouse gp10025-33 peptides. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00586 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 05 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Lea Eisenbach, Weizmann Institute of Science, Rehovot, Israel, lea.eisenbach@weizmann.ac.il Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Gal Cafri Adi Sharbi-Yunger Esther Tzehoval Zoya Alteber Meir Azulay Yosi M Gozlan Lea Eisenbach Google Gal Cafri Adi Sharbi-Yunger Esther Tzehoval Zoya Alteber Meir Azulay Yosi M Gozlan Lea Eisenbach Google Scholar Gal Cafri Adi Sharbi-Yunger Esther Tzehoval Zoya Alteber Meir Azulay Yosi M Gozlan Lea Eisenbach PubMed Gal Cafri Adi Sharbi-Yunger Esther Tzehoval Zoya Alteber Meir Azulay Yosi M Gozlan Lea Eisenbach Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
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