Conventional Dishes Research Articles

Mengembangkan Inspirasi Kewirausahaan dan Kualitas Perdagangan Skala Kecil Melalui Manajemen Aset Manusia

Within the confront of fast worldwide nourishment patterns and modernization, concerns are rising almost the potential misfortune of nearby culinary conventions that exemplify a nation's imaginativeness, culture, history, and character. To address this, STIEPARI Semarang propelled a community benefit extend in Nongkosawit, Gunungpati, pointed at protecting and advancing its one of a kind culinary legacy. The venture included recording conventional formulas and their social centrality, guaranteeing that this information is passed down and shared. The group collaborated with neighborhood cooks to coordinated present day culinary methods whereas keeping up genuineness. Workshops were held to educate the community how to showcase their conventional dishes, emphasizing storytelling as a way to associate with visitors. To maintain these endeavors, the venture presented computerized stages for nearby culinary businesses to reach broader gatherings of people through social media and e-commerce. This activity not as it were jam Nongkosawit s culinary bequest but moreover engages the neighborhood community by making modern financial openings tied to their wealthy social conventions. Throughthese endeavors, Nongkosawit Town represents the significance of defending and celebrating culinary conventions that characterize our social personality.

P-380 Improvement of follicle growth and health during dynamic culture of bovine ovarian cortical tissue (BOCT) is associated to a higher remodeling of the extracellular matrix

Abstract Study question Is improvement of follicle growth and health during dynamic culture in Perifusion Bioreactor (PB) associated to a healthier condition and remodeling of extracellular matrix? Summary answer The dynamic culture in PB allows the extracellular matrix (ECM) network rearrangement improving follicle growth and viability compared to static culture What is known already Over the past two decades, in vitro folliculogenesis has achieved moderate success in large mammals, emerging as a distinctive and reliable method for obtaining secondary follicles. Recently, although dynamic culture in bioreactors has been demonstrated to enhance follicle progression, quality, and viability compared to static culture, less emphasis has been devoted to ECM remodeling. Ovarian tissue ECM plays a crucial role in cell–cell, and cell-ECM interactions involved in follicle activation and growth. The comprehension of ECM function deepens our understanding of follicular development and holds significant promise for future reproductive technologies Study design, size, duration Bovine ovaries (age 8-24 months) were collected at slaughterhouse. BOCT strips (1x1x0.5mm) from the same ovary were cultured in groups of 10 for 14 days in PB (dynamic culture) and conventional dishes (CD, static culture). At the end of culture, collagen thickness and packing density was assessed through PicroSirius red (PSR) staining, follicle stages and quality through histology, and follicle viability through live-dead far-red and propidium iodide labeling under confocal microscopy Participants/materials, setting, methods Neosynthesis and remodeling of collagen fibers was analyzed on PSR-stained samples under polarized light measuring the color of each pixel. A color threshold was set to isolate the four colors seen in PSR-stained samples under polarized light: green, thin fibers (neosynthesized); yellow, mid-sized fibers (low assembly); orange, mid-sized fibers (medium assembly); red, mature thick fibers (high assembly). The color threshold was set as follows: red 2–9, yellow 39-51, orange 10–38, green 52–128 Main results and the role of chance In fresh tissue, analysis of PSR-stained samples showed the following values: Red 1.35; Orange 4.93; Yellow 0.18; Green 0.15. At Day 14, compared to the static culture, culture in PB, exhibits a significantly higher production of green (PB 0.22 vs CD 0.02 P < 0.01) and yellow (PB 0.56 vs CD 0.26 P < 0.05) fibers indicating an improved neosynthesis and remodeling of collagen and ECM health condition. Such higher ECM remodeling after dynamic culture is associated to a significantly higher percentage of secondary follicles (PB 15.4– CD 4.1%, P < 0.01 ), superior follicle quality (grade I/II, PB 70,5 - CD 28.4%, P < 0.01) and viability (PB 70 – CD 41.1%, P < 0.01). PSR analysis suggests that the continuous nutrients and oxygen supply, catabolites removal, and biomechanical stimulation during dynamic PB culture, stimulates the deposition of newly synthesized collagen which in turn, promotes follicle growth Limitations, reasons for caution Although the bovine is a suitable reproductive model for human, such findings should be replicated on human ovarian tissue Wider implications of the findings The aim of ovarian tissue culture is to promote the growth of healthy secondary follicles that can be isolated for further culture to generate MII oocytes. The present findings suggest that adoption of dynamic ovarian tissue culture could enhance the outcome of folliculogenesis in vitro Trial registration number not applicable

Penggabungan Kearifan Lokal dan Teknopreneurship dalam Wisata Kuliner Desa Nongkosawit Gunungpati Semarang

Within the confront of fast worldwide nourishment patterns and modernization, concerns are rising almost the potential misfortune of nearby culinary conventions that exemplify a nation's imaginativeness, culture, history, and character. To address this, STIEPARI Semarang propelled a community benefit extend in Nongkosawit, Gunungpati, pointed at protecting and advancing its one of a kind culinary legacy. The venture included recording conventional formulas and their social centrality, guaranteeing that this information is passed down and shared. The group collaborated with neighborhood cooks to coordinated present day culinary methods whereas keeping up genuineness. Workshops were held to educate the community how to showcase their conventional dishes, emphasizing storytelling as a way to associate with visitors. To maintain these endeavors, the venture presented computerized stages for nearby culinary businesses to reach broader gatherings of people through social media and e-commerce. This activity not as it were jam Nongkosawit s culinary bequest but moreover engages the neighborhood community by making modern financial openings tied to their wealthy social conventions. Through these endeavors, Nongkosawit Town represents the significance of defending and celebrating culinary conventions that characterize our social personality.

P-412 Long-term in vitro dynamic culture of bovine ovarian cortical tissue (BOCT) increases follicle growth, viability and AMH secretion

Abstract Study question Does improved oxygen availability and biomechanical stimulation during long-term dynamic in vitro culture of BOCT enhance secondary follicles growth and health? Summary answer Long-term dynamic versus static in vitro culture of BOCT increases the proportion, viability and AMH secretion of growing follicles. What is known already A limiting factor in multistep in vitro folliculogenesis is the low yeld of healthy secondary follicles after ovarian cortical tissue culture. We previously reported that dynamic in vitro culture of ovarian cortical tissue for 7 days in a newly designed perifusion bioreactor (patent 102020000027290) enhances follicle growth and health compared to conventional static culture. The bovine represents a valuable animal model to study early folliculogenesis in vitro. Study design, size, duration Bovine ovaries (age 8-24 months) were collected from slaughterhouse. BOCT strips from the same ovary were cultured for 14 days in perifusion bioreactors (PB, dynamic culture) and conventional dishes (CD, static culture). BOCT cultured in both PB and CD was monitored by collecting spent media every 2 days and through histology, live-dead confocal analysis at the end of culture. Participants/materials, setting, methods BOCT slices (0.5mm thick) were obtained by a custom tissue slicer, chopped into 1x1mm strips and cultured in group of 10 under in PB and CD. Follicle stages and quality in fresh (D0) and cultured tissues were evaluated by histology (hematoxylin-eosin staining), follicle viability was estimated by labelling with live-dead far-red and propidium iodide at the confocal microscope. Whole tissue viability (spectrophotometric LDH assays) and AMH secretion (mass spectrometry) were evaluated on spent media. Main results and the role of chance Overall, 939 follicles were analyzed (histology, 338; viability, 601). Day 0-Follicle stages: primordial, 82.4%; primary, 13.2%; secondary, 4.4%; follicle quality: grade 1-2, 37,6%; grade 3, 62,4%; live follicles: 94.3%. Day 14: PB vs CD - Follicle stages: primordial, 3.8 vs 5.7%,NS; primary, 75 vs 86.1%, NS; secondary, 21.2 vs 8.2%, P < 0.05; follicle quality: grade I/II, 70 vs 18.9%, P < 0.01; grade III, 30 vs 81.1%, P < 0.01; live follicles 62.6 vs 35.3%, P < 0.01. LDH activity in PB was markedly lower than in CD at all culture times (PB14d 150.5 nmol vs CD14d 233.1 nmol). During 14 days culture, AMH secretion continuously increases in PB (1700 picomol/Vtot) whereas in CD it markedly decreases during culture, falling down at very low values at the end of culture (67 picomol/Vtot). Findings indicate that the increased transport of solutes and dissolved oxygen, and the biomechanical stimulation in our novel dynamic bioreactor are key factors during ovarian tissue culture. Analysis of LDH and AMH levels in spent media confirm that dynamic culture better mantains general tissue health and promote a better functionality of growing follicles. Limitations, reasons for caution Although the bovine is considered a reliable model for human folliculogenesis, the study should be validated on human ovarian tissue. Wider implications of the findings Poor yield of secondary follicles during organ culture is a limiting step in the in vitro production of mature oocytes from primordial follicles. The use of a newly developed dynamic bioreactor for long-term culture could represent a valuable tool for in vitro multistep folliculogenesis. Trial registration number not applicable

Cellular population dynamics shape the route to human pluripotency

Human cellular reprogramming to induced pluripotency is still an inefficient process, which has hindered studying the role of critical intermediate stages. Here we take advantage of high efficiency reprogramming in microfluidics and temporal multi-omics to identify and resolve distinct sub-populations and their interactions. We perform secretome analysis and single-cell transcriptomics to show functional extrinsic pathways of protein communication between reprogramming sub-populations and the re-shaping of a permissive extracellular environment. We pinpoint the HGF/MET/STAT3 axis as a potent enhancer of reprogramming, which acts via HGF accumulation within the confined system of microfluidics, and in conventional dishes needs to be supplied exogenously to enhance efficiency. Our data suggest that human cellular reprogramming is a transcription factor-driven process that it is deeply dependent on extracellular context and cell population determinants.

P-447 Enhancing oxygen availability in the dynamic culture of bovine ovarian cortical tissue improves the yield of secondary follicles

Abstract Study question Does enhancing oxygen availability during dynamic in vitro culture of bovine ovarian cortical tissue (BOCT) improve follicle growth and health? Summary answer Enhancing oxygen availability during dynamic in vitro culture of BOCT in perifusion bioreactors (PB) does improve follicle health and yield to secondary follicles What is known already Oxygen availability has been demonstrated to represent a key factor in follicle health and growth during in vitro culture of bovine and human ovarian cortical tissue (HOCT) under static culture conditions. Disruption of solutes gradients and application of physiological fluid mechanical stress, through in vitro dynamic culture of HOCT in a newly designed perifusion bioreactor have been shown to further enhance follicle growth and health. As it shows striking similarities with human, bovine folliculogenesis is considered a valuable model to study follicle growth in vitro Study design, size, duration Bovine ovaries from animals aged 8-24 months were collected at slaughterhouse. In each experiment (n = 3), BOCT strips from the same ovary were cultured for 6 days in perifusion bioreactors (PB, dynamic culture) and conventional dishes (CD, static culture). Culture outcome in static culture was analysed and compared to two bioreactor configurations in which medium oxygenation was kept low by using a standard tube reservoir (StPB) or was enhanced by using a gas-permeable dish reservoir (PB+O2). Participants/materials, setting, methods Slices of BOCT 0.5mm thick were cut with a tissue slicer and chopped into 1x1mm strips. In each experiment, fresh (D0) and cultured tissue (groups of ten strips) were analyzed. Follicle stages and health were assessed by histology (hematoxylin-eosin staining). Follicle viability was estimated by labelling with live-dead far-red and propidium iodide followed by clearing before analysis at the confocal laser scanning microscope. Main results and the role of chance Overall, 2417 follicles were analyzed (histology, 1476; viability, 941). At day 0 most follicles were primordial (primordial, 88.7%; primary, 10.6%; secondary, 0.7%), and had good quality (grade 1-2, 92.2%; grade 3, 7.8%), and high viability (91.8%). At day 6, follicle growth and health in StPB was superior than in CD (StPB vs CD - staging: primordial, 6.8 vs 16.3, P < 0.01; primary, 70.7 vs 74.1, NS; secondary, 22.5 vs 9.6%, P < 0.01; grading: grade 1 + 2, 71.4 vs 44.8, P < 0.01; grade 3, 28.6 vs 55.2%, P < 0.01). Dynamic culture in StPB better-preserved follicle viability compared to static culture in CD (StPB vs CD: 77.75 vs 64.9%, P < 0.01). Enhancing oxygen availability during dynamic culture increased follicle progression and viability (PB+O2 vs StPB - staging: primordial, 5.1 vs 6.8, NS; primary, 65.4 vs 70.7, NS; secondary, 29.5 vs 22.5%, P < 0.05; viability - 92 vs 77.75, P < 0.01). Overall, the obtained results demonstrate that i) disruption of stagnant layers of medium and application of shear stress to BOCT through dynamic culture improves follicle activation, growth and health; ii) enhancing oxygen availability by means of a gas-permeable medium reservoir further increases follicle progression and viability. Limitations, reasons for caution Although the bovine is considered a reliable model for human folliculogenesis, the study should be validated on human ovarian tissue. Wider implications of the findings A limiting step in the in vitro production of mature oocytes starting from primordial follicles is the low yield of secondary follicles after organ culture. The adoption of a newly designed dynamic bioreactor and modulation of oxygen availability could represent a valuable tool for multistep in vitro folliculogenesis. Trial registration number none

Neuromarketing in Haute Cuisine Gastronomic Experiences

Gastronomic experiences offer a set of stimuli that affect the customer’s perception of chef-designed food. This empirical study aims to analyze the influence on the consumer, at a cerebral level, of the stimuli characteristic of a high-level gastronomic experience, in a Michelin starred restaurant. The presentation by the waiter or chef, the plate design, the dish served, the taste of food, interaction or moment in which the food is served are the variables analyzed. Through the use of neuromarketing techniques – galvanic skin response to register emotional arousal, eye tracking to identify where consumers look, and electroencephalography to interpret emotional reactions – combined with qualitative research technique (In-depth interviews with all consumers), in order to know the natural and suggested memories, the objective of this research is to determine the emotional impact of the variables analyzed against the actual taste of food, obtaining conclusions about each variable in overall experience and allowing the authors to propose a model of order design of dishes, designed by the chef, based on emotions and thereby achieving greater efficiency in results of the experience and the memory of it. Results indicate a favorable influence on emotions when the chef presents the food. Likewise, dishes with special presentation have a greater influence at the level of interest than conventional dishes. It is important to highlight that the levels of emotion and attention fall after the midway point of the experience, due to the duration of the experience. Therefore, the dishes do not have the same emotional impact, despite being as special as at the beginning of the experience.

Methodology for design of suitable dishes for dysphagic people

A methodology to adapt dishes for cerebral palsy (CP) dysphagic people was developed. Five conventional dishes were cooked, blended and texturized with mixtures of thickeners and gelling agents based on xanthan gum. The most appropriate texturizing agents were selected, the textural thermostability of the dishes was studied, and the shelf-life was evaluated by back extrusion, sensory and microbiology analysis. Information about the acceptability of the adapted dishes by CP dysphagic people was obtained through the control of the consumed fraction and the liking or disliking reaction after eating the dishes. The adapted dishes considered suitable for swallowing process showed maximum force between 6.2(0.1) N and 18.9(3.3) N, minimum force between −3.9(0.3) N and −9.2(1.3) N, and Fmin/Fmax ratio between 0.4(0.0) and 0.7(0.0). Regarding sensory characteristics, the adapted dishes showed low stickiness, residue and firmness, and high suitable texture score. The methodology was appropriate for developing suitable dishes, sensorially accepted by dysphagic people, thermostable for 7 days refrigerated storage and ready-to-eat. Industrial relevanceThis study presents the technological basis for the standardized design of suitable ready-to-eat dishes for dysphagic people. The methodology developed is of great interest to the industry of ready-to-eat dishes.

Abstract 1891: NF-κB signaling plays a certain role in apoptosis resistance in 3D-cultured EGFR-mutant lung adenocarcinoma cells

Abstract It is generally thought that suspended tumor cells show reduced proliferative activity and are more resistant to conventional chemotherapy compared with adherent tumor cells. Two epidermal growth factor receptor (EGFR)-mutant lung adenocarcinoma cell lines, HCC827 (E746-A750 deletion) and H1975 (L858R + T790M), cultured in suspension, however, undergo apoptosis much more easily than these two cell lines cultured in conventional monolayer dishes, i.e. anchorage dependent conditions, when the kinase activity of EGFR is completely inhibited by treatment with EGFR tyrosine kinase inhibitors (TKIs) regardless of suspension or monolayer culture conditions. These findings indicate that attached EGFR-mutant lung adenocarcinoma cells appear less dependent on constitutive EGFR signaling for survival than detached cells, probably because integrin ligation enhances cell survival in adherent cells. However, the microenvironment of cells in monolayer culture conditions is considerably different from that of cells in vivo. We thus tested whether EGFR-mutated lung adenocarcinoma cells in three dimensional (3D) conditions using Matrigel resist EGFR TKIs-induced apoptosis compared with suspended cells. We uncovered three major findings in this study as follows: 1) both HCC827 cells and H1975 cells cultured in 3D conditions were significantly less sensitive to EGFR TKIs-induced apoptosis than suspended cells. 2) Marked degradation of IαBβ, the inhibitor of NF-κB pathway, was observed only in 3D-cultured EGFR-mutated lung adenocarcinoma cells, resulting in NF-κB signaling activation. 3) TPCA-1, a potent IKKα inhibitor, can enhance the levels of apoptosis in 3D-cultured EGFR-mutant cells treated with EGFR TKIs. These results suggest that combined EGFR and NF-κB inhibition is required to induce apoptosis effectively in EGFR-mutated lung adenocarcinoma cells in vivo. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1891. doi:1538-7445.AM2012-1891

Application of collagen coated silicone scaffolds for the three‐dimensional cell culture of primary rat hepatocytes

Primary hepatocytes present an appropriate validation system for toxicological studies of novel agents in pharmaceutical‐ and cosmetics industries. Until today, three dimensional culture systems maintaining the functionality of hepatocytes are missing.The aim was to analyse the applicability of 3D collagen‐coated silicone scaffolds versus 2D culture for culture of rat hepatocytes.Primary rat hepatocytes were cultivated for 72h on multilayer lattice structures (A=23.7cm2) and compared with 2D culture on conventional dishes (A=8.9cm2). Cell counts, the growth pattern on silicone and the urea production rate were determined.Based on identical initial cell counts the growth rate was about 42%±5% higher in 3D culture as compared to 2D. Under serum‐free conditions cells attached to the scaffold, formed clusters and organ‐like structures. The 3D culture on the silicone scaffold displayed no significant cytotoxicity after 72h: the percentages of dead cells were 7%±3% vs 9%±3%. The urea production rate [μg/105 cells] was significantly higher in the 3D vs 2D: 24h 9.1±2.7 vs 4.7±0.2; 48h 5.7±1.5 vs 3.7±0.05 72h 3.5±0.5 vs 2.1±0.1.The results show the appropriateness of silicone scaffolds for the 3D culture of primary rat hepatocytes. Thus, the collagen‐coated silicone scaffolds represent an important tool for the development of 3D hepatocyte cultures sustaining the physiological functions.

A high-density culture of hepatocytes using porous substrate for use as a bioartificial liver

High-density, large-scale culture of hepatocytes is a key requirement in the development of a bioartificial liver that can replace liver functions in patients with severe liver insufficiency. We have applied a porous polymer, polyvinyl formal (PVF) resin, as a cell-supporting material for hepatocyte culture. We evaluated the performance of the culture system using PVF resin under three different culture conditions: a shake culture on conventional dishes, perfusion culture with sheet-shaped PVF, and a packed-bed-type module. Among them, the packed-bed reactor using PVF resin enabled high-density culture of hepatocytes (2×107 cells/cm3-PVF). The hepatocytes immobilized in the PVF resin maintained satisfactory metabolic functions (ammonium metabolism and albumin secretion) comparable to those of the monolayer dish cultures. Furthermore, by maintaining dissolved oxygen concentration at a relatively high level (260–460μM), the metabolic functions of the hepatocytes were improved. It was concluded that the packed-bed reactor using PVF resin is a promising system for developing a bioartificial liver using hepatocytes.

Pistic, traditional food from Western Friuli, N.E. Italy

Western Friuli, Italy, there is a small area near the town of Pordenone where an ancient rite of spring is still carried out. This is the preparation of a special dish, known as “pistic,” a collection of 56wild herbaceous meadow and wood plants which are boiled and then sauteed together. This practice is still alive in a few areas of Friuli today and possibly goes back to pre-Roman Celtic cultures in this part of Friuli. The number of herbaceous plants used in this dish is extraordinarily high (56), especially when compared to the low number normally used in other conventional dishes. “Pistic” is therefore important, not only because it represents a quantitatively high use of wild herbs in the diet of the rural population, but also because it reflects environmental awareness, in that the archaic method of naming, identifying and using these plants still exists today. Similar rural practices include the use of “pot herbs” in Great Britain and in France the cooking of “mesclun.”

Altered response to dacarbazine by murine melanoma cells cultured in type I collagen gel

The effects of antitumor agents are usually assayed on soft agar culture or monolayer culture on plastic. However, such an environmental condition cannot be found in vivo and could be too severe for cells to survive. Recently, studies have indicated that culture of cells in or on a collagen gel provides a matrix-like environment and enables the cells to exhibit fully their differentiating functions not observed on plastic [1, 4, 9, 11, 12]. To evaluate the effect of antitumor drugs under the conditions resembling those occurring in the process of infiltrating the dermis in vivo as much as possible, we cultured B16 melanoma cells in type I collagen gel and compared the effects of dacarbazine (DTIC) [3, 8] and all-trans-retinoic acid (RA) [7] with those on the conventional plastic dishes. Three-dimensional culture in type I collagen gel was performed according to the method of Elsdale and Bard [4] after modification as described previously [6]. Briefly, 0.3% pepsinized type I collagen solution (derived from porcine tendon, Nitta Gelatine, Osaka, Japan), HEPES/ NaOH neutralizing solution, concentrated Eagle's medium, cell suspension, and fetal calf serum (FCS) were mixed at below 4 ~ C. One millilitre of the mixture was poured into a 35-ram bacteriological dish (Falcon) and incubated at 37 ~ C. The final collagen concentration was 2.0 mg/ml and the initial cell density was 2 x 10 s cells/ml. After gelling, 2 ml of Eagle's medium supplemented with 10% FCS was added. Addition of DTIC or RA was started on day 4. DTIC was activated with exposure to white light [8]. Culture medium supplemented with FCS and these reagents was changed every other day. At indicated times gels were digested by collagenase at 37 ~ C for 40 rain and released viable cells were counted with a hemocytometer using the dye exclusion test.

Die Lupine, ein Beitrag zur Nahrungsversorgung in den Anden 4. Die Akzeptabilität des Mehles von Lupinus albus

48 different dishes enriched with lupinus albus flour have been tested by 20 persons. The test duration has been 4 weeks. Meals and products with lupine admixtures have been judged equally with concern to corresponding conventional dishes. Farinaceous products had the best test results. Therefore, it is recommended for Peru to add lupine flour when producing bread, biscuits, sauces, soups, and noodles (maccaronis etc.). The test time had no influence to the test results.