Conventional Assembly Research Articles

Electrostatic Assembly of Gold Nanoclusters in Reverse Emulsion Enabling Nanoassemblies with Tunable Structure and Size for Enhanced NIR-II Fluorescence Imaging.

The precise control of the assembly structure and size of gold nanoclusters (AuNCs) can potentially amplify their near-infrared II (NIR-II) fluorescence imaging and targeting properties. However, the conventional electrostatic assembly of AuNCs and charged molecules faces challenges in balancing the inherent electrostatic repulsions among charged units and regulating the diffusion of assembly units. These difficulties limit precise control over assembly size and structure, along with limited options for coassembled molecules, thereby restricting imaging properties and targeting capability. To circumvent this challenge, we developed a reverse emulsion-confined electrostatic assembly method. This technique efficiently constructs AuNC nanoassemblies with diverse coassembled molecules, allowing for the fine-tuning of assembly size and structure, including both core-satellite and homogeneous AuNC nanoassemblies. The development of two distinct nanoassemblies can be partially attributed to the varying diffusive rates of AuNCs or the AuNCs/polymer complex within the fused emulsion droplets. This variance arises from steric hindrances encountered during the emulsion fusion process. Interestingly, core-satellite nanoassemblies exhibit the strongest NIR-II fluorescence enhancement. Finally, the introduction of a hyaluronic acid coating on the surfaces of nanoassemblies with varying sizes enables the nanoprobes to achieve enhanced lymph node imaging through size modulation and macrophage targeting, which are used for surgical navigation to remove lymph node metastases. We envision that this self-assembly strategy can be extended to a wide range of electrostatic assembly systems for the development of multicomponent functional materials.

Enzyme-accelerated catalytic DNA circuits enable rapid and one-pot detection of bacterial pathogens

Catalytic DNA circuits, serving as signal amplification strategies, can enable simple and accurate detection of pathogenic bacteria in complex matrices but suffer from low reaction rates and depths. Herein, we design an enzyme-accelerated catalytic hairpin assembly (EACHA) in which duplex DNA products are converted into hairpin reactants to continue participating in the next circuit reaction with the assistance of RNase H. Profiting from the high recyclability of the reactants, EACHA exhibits an approximately 37.6-fold enhancement in the rate constant and a two-order-of-magnitude improvement in sensitivity compared to conventional catalytic hairpin assembly (CHA). By integrating an allosteric probe with EACHA, a one-pot method is developed for rapid and direct detection of S. enterica Enteritidis (S. Enteritidis). This method is capable of detecting 15 CFU mL−1 of S. Enteritidis within 20 min, which is superior to that of real-time PCR. By testing 60 milk samples, we demonstrate this method's high accuracy in discriminating contaminated samples, with an area under the curve (AUC) of 0.997. Moreover, this method can be employed to accurately diagnose early-stage infected mice, with an AUC of 1.00 for feces samples and 0.986 for serum samples. Therefore, this study offers a simple and feasible method for identifying pathogens in complex matrices.

Diffusion-Bonded Unitized Anode Assemblies for PEM Electrolysis Stacks

Hydrogen generation via proton exchange membrane (PEM) water electrolysis is in the midst of a technological renaissance aimed at achieving cost parity with steam reforming within the decade. Reaching that plateau will require substantial system cost reductions accompanied by increased performance of the cell stack module. Many proposed improvements to the cell stack draw from the established principles of electrochemical and manufacturing engineering, targeting lower component resistances while lowering part count and enhancing ease of assembly, respectively.The PEM electrolyzer anode assembly (comprising the porous transport layer (PTL) and the anode flow field) is suited for just such a revision. In practice, the anode PTL is positioned on a metallic bipolar plate that is machined or embossed with a flow field, or the PTL is joined to an assemblage of thin macroporous media that serves as a flow field1. In both cases, the interfacial resistances between the subcomponents of the assembly are non-negligible and are conventionally mitigated by applying noble metal coatings, typically Pt.In this contribution, we investigate alternatives to conventional assemblies via diffusion bonding. Using thermal treatments, the porous transport layer is joined to the anode flow field, yielding a robust, atomic-scale metallurgical bond. We demonstrate the manufacturing of large-format assemblies in multiple configurations, using either stacked microporous media or grooved plates, meeting tight tolerances for thickness across their span. We also show that the bulk-like character imparted to the assembly obviates the need for antipassivation coatings at the critical interfaces between components, evinced by low series resistance in both ex situ and in situ electrolysis cell tests.References(1) Dristy, M. E. Proton Exchange Membrane Electrochemical Cell System. US7270908B2, September 18, 2007.

Pressure generation under deformation in a large-volume press

Abstract Deformation can change the transition pathway of materials under high pressure, thus significantly affects physical and chemical properties of matters. However, accurate pressure calibration under deformation is challenging and thereby causes relatively large pressure uncertainties in deformation experiments, resulting in the synthesis of complex multiphase materials. Here, pressure generations of three types of deformation assemblies were well calibrated in a Walker-type large-volume press (LVP) by electrical resistance measurements combined with finite element simulations (FESs). Hard Al2O3 or diamond pistons in shear and uniaxial deformation assemblies significantly increases the efficiency of pressure generation compared with the conventional quasi-hydrostatic assembly. The uniaxial deformation assembly using flat diamond pistons possess the highest efficiency in these deformation assemblies. This finding is further confirmed by stress distribution analysis based on FESs. With this deformation assembly, we found shear can effectively promote the transformation of C60 into diamond under high pressure and realized the synthesis of pure-phase diamond at relatively moderate pressure and temperature conditions. The present developed techniques will help improve pressure efficiencies in LVP and explore the new physical and chemical properties of materials under deformation in both science and technology.

Distillation of treated dairy effluent using integrated solar dish collector and solar still: A case study of distillate output enhancement

A solar thermal distillation system was designed to be compatible with electrically powered conventional laboratory distillation assembly. Its efficacy was checked in converting the treated dairy effluent into distilled water. The experimental setup comprised of a solar parabolic dish collector (aperture area 4.15 m2) and a solar still (basin area 1 m2) and was designed such that both can be used separately or in the combination. The study was conducted during March – June 2023. The solar dish collector was tested with two receivers with different bottom materials viz., stainless-steel and brass, thereby an average daily yield of 6.5 litres and 6.9 litres were obtained for both receivers, respectively. Simultaneously, solar still produced 2.08 litres of water individually per day. Thus, the highest yield obtained for whole setup was 8.9 litres per day considering individual performances of both the systems. The daily average thermal efficiency of dish collector for was found 65.59 % with steel receiver and 63.33 % with brass receiver. Similarly, thermal efficiency of solar still was 35 %. The quality of distillate was analysed on 10 parameters viz., pH, TDS, TSS, COD, BOD, Oil & Grease, SPC, Coliform count, electrical conductivity, and chloride content which showed promising results.

An electromechanically driven dielectric elastomer based tunable reflector

Deformable optics offer numerous advantages over conventional optical assemblies, including compactness, cost-effectiveness, efficiency, and flexibility. This study focuses on a reflector based on dielectric elastomer actuators (DEAs) with an internal fluid (air) coupling. DEAs are a class of electroactive materials adept at accommodating substantial actuation strains and rapid responses. Fluid distributed between the active and passive parts remains entirely enclosed by the device and transmits actuation pneumatically. Dynamic maneuvers conducted through a series of controlled electrical signals demonstrate proper control over optical characteristics. However, DEs exhibit inherent flaws in dynamic actuation, referred to as instabilities, which are mitigated by applying an initial pre-stretch. The study identifies optimal parameters that confer stability to the reflector: minimum to no creep, zero residual vibrations, and low viscous losses. An analytical framework is developed to assess device performance, focusing on the spherical curvature assumption that closely resembles the behavior of tunable spherical reflectors. Additionally, an optical bench setup is employed to demonstrate the relationship between focal length and applied pressure. Notably, this paper underscores the potential of a DE-based variable focal length reflector to function effectively within a dynamic environment.

A Thermally Enhanced Film Adhesive for Assembling High Power Density Electronic Devices

Assembly adhesives play a crucial role in packaging and thermal management design for microelectronics devices. The main function of the adhesives is attaching multiple components together to deliver designed functionalities and ensure integrity and reliability of the device. As requirements for performance and functionality increase, heat generated from these devices increases exponentially. For example, local heat flux can easily exceed 50 W/cm2 for IGBT power modules while it may reach several hundred W/cm2 at active areas for Gallium Nitride (GaN) power amplifiers. However, intrinsic thermal conductivity of polymer-based adhesives is around 0.2 W/mK, which increasingly becomes a thermal bottleneck in high power density electronics devices, especially those used in defense and aerospace systems. Effectively and safely dissipating the high heat and ensuring device reliability have become one of the critical tasks in the development of modern aerospace and defense systems. This paper presents an adhesive film that uses non-electrically conductive particles with high thermal conductivity as fillers in the adhesive to enhance thermal transport. The adhesive film offers an effective thermal conductivity around 0.7 W/mK, which is more than a 3X improvement over conventional assembly adhesives. In addition, the film adhesive has a long work life at ambient conditions with significantly improved processability and controllability, such as bondline thickness control and uniformity, no voids/air pockets, no or minimum resin bleed, etc. They can also be easily converted into preforms with complicated shapes/geometries with typical tolerances less than 10 mil to facilitate assembly processes and ensure a clean and waste free environment.

Protein-Assisted Large-Scale Assembly and Differential Patterning of DNA Origami Lattices.

Nanofabrication has experienced a big boost with the invention of DNA origami, enabling the production and assembly of complex nanoscale structures that may be able to unlock fully new functionalities in biology and beyond. The remarkable precision with which these structures can be designed and produced is, however, not yet matched by their assembly dynamics, which can be extremely slow, particularly when attached to biological templates, such as membranes. Here, the rapid and controlled formation of DNA origami lattices on the scale of hundreds of micrometers in as little as 30minutes is demonstrated, utilizing active patterning by the E.coli Min protein system, thereby yielding a remarkable improvement over conventional passive diffusion-based assembly methods. Various patterns, including spots, inverse spots, mazes, and meshes can be produced at different scales, tailored through the shape and density of the assembled structures. The differential positioning accomplished by Min-induced diffusiophoresis even allows the introduction of "pseudo-colors", i.e., complex core-shell patterns, by simultaneously patterning different DNA origami species. Beyond the targeted functionalization of biological surfaces, this approach may also be promising for applications in plasmonics, catalysis, and molecular sensing.

Enhancing electrochemomechanics: How stack pressure regulation affects all-solid-state batteries

Stack pressure application in solid-state batteries (SSBs) is crucial for achieving high-energy density by promoting interfacial contact. Fluctuations in stack pressure at the MPa-scale can result in mechanical fatigue, leading to the degradation of materials within a fixed-volume cell casing. Thus, it is essential to regulate these stack pressure variations during cycling. In this study, we successfully stabilize the evolution of stack pressure at the hundred kPa-scale by incorporating compression springs into the conventional SSB assembly. This kPa-level stabilization is achieved by converting elastic potential energy into spring deformation. We investigate these mechanical responses by correlating them with stack pressure and cell thickness measurements in a variable volume cell. Furthermore, accommodating volume changes results in more than 98 % retention of the highest stack pressure retention. These findings can significantly contribute to advancements in cell assembly processes critical for scaling up SSB modules.

Accelerated Recombination of Lophyl Radicals in Micelles: Rapid Controlled Self-Assembly of Micelles Formed by Amphiphilic Lophine Dimers and Release of Solubilized Substance by Photoirradiation.

Controlling the morphology of molecular assemblies formed by surfactants by photoirradiation enables the controlled release of incorporated substances, which can be applied to delivery systems for drugs and active ingredients. On the other hand, conventional photoresponsive surfactants and molecular assemblies have a slow response speed, making it difficult to control their functions at the desired time. In this review, I discuss our recent progress in the accelerated control of functions of photoresponsive molecular assemblies by using lophine dimer as a photochromic compound. The lophine dimer derivative dissociates into a pair of lophyl radicals that upon ultraviolet (UV) light irradiation, and these radical species thermally recombine although the recombination reaction is extremely slow due to the diffusion of lophyl radicals. By using the confined inner space of micelles formed by surfactants, the recombination reaction was extremely accelerated. With UV light irradiation, rapid morphological changes in micelles, formed by amphiphilic lophine dimers were observed by using in situ small-angle neutron scattering (in situ SANS) system. Moreover, the rapid controlled release of calcein as a model drug was achieved by UV light irradiation using the photoresponsive micelles. This rapid system can realize the controlled release of drugs truly at the desired time, developing an efficient and precise drug delivery system (DDS). Furthermore, it can be applied in a wide range of fields such as release control of active ingredients, efficient heat exchange control, and actuating systems.

CryoEM reveals the complex self-assembly of a chemically driven disulfide hydrogel.

Inspired by the adaptability of biological materials, a variety of synthetic, chemically driven self-assembly processes have been developed that result in the transient formation of supramolecular structures. These structures form through two simultaneous reactions, forward and backward, which generate and consume a molecule that undergoes self-assembly. The dynamics of these assembly processes have been shown to differ from conventional thermodynamically stable molecular assemblies. However, the evolution of nanoscale morphologies in chemically driven self-assembly and how they compare to conventional assemblies has not been resolved. Here, we use a chemically driven redox system to separately carry out the forward and backward reactions. We analyze the forward and backward reactions both sequentially and synchronously with time-resolved cryogenic transmission electron microscopy (cryoEM). Quantitative image analysis shows that the synchronous process is more complex and heterogeneous than the sequential process. Our key finding is that a thermodynamically unstable stacked nanorod phase, briefly observed in the backward reaction, is sustained for ∼6 hours in the synchronous process. Kinetic Monte Carlo modeling show that the synchronous process is driven by multiple cycles of assembly and disassembly. The collective data suggest that chemically driven self-assembly can create sustained morphologies not seen in thermodynamically stable assemblies by kinetically stabilizing transient intermediates. This finding provides plausible design principles to develop and optimize supramolecular materials with novel properties.

A New Bottom-Hole Assembly Design Method to Maintain Verticality and Reduce Lateral Vibration

Well deviation is a prevalent problem in deep oil and gas exploration, leading to a significant increase in drilling costs. The conventional bottom-hole assembly (BHA) anti-deviation design method does not consider the impact of the BHA structure on lateral vibration. This paper proposes an integrated BHA design method that takes into account both anti-deviation and vibration reduction. This method evaluates the BHA’s anti-deviation ability using the drilling trend angle. A negative value of the drilling trend angle indicates that the BHA can correct well deviation. A finite element linearized dynamics method is used to evaluate the lateral vibration intensity of the BHA. This method involves calculating the bending displacement caused by mass imbalance and then determining the magnitude of the bending strain energy based on this displacement. The structural factors affecting the anti-deviation ability and potential lateral vibration intensity of pendulum BHAs and bent-housing mud motor (BHMM) BHAs were studied, and field tests were conducted for verification. The research shows that for pendulum BHAs, the factor that has the greatest impact on anti-deviation ability and vibration intensity is the distance from the stabilizer to the drill bit. For BHMM BHAs, the length of the short drill collar has a significant impact on the vibration intensity. Compared with current design methods, the mechanical specific energy (MSE) of the single stabilizer pendulum BHA decreased by 12%, while the MSE of the BHMM BHA decreased by 26.4%. Both decreases indicate a reduction in vibration intensity. This study will help to further increase drilling speed while preventing well deviation.

Solvent-pair surfactants enabled assembly of clusters and copolymers towards programmed mesoporous metal oxides

Organic-inorganic molecular assembly has led to numerous nano/mesostructured materials with fantastic properties, but it is dependent on and limited to the direct interaction between host organic structure-directing molecules and guest inorganic species. Here, we report a “solvent-pair surfactants” enabled assembly (SPEA) method to achieve a general synthesis of mesostructured materials requiring no direct host-guest interaction. Taking the synthesis of mesoporous metal oxides as an example, the dimethylformamide/water solvent pairs behave as surfactants and induce the formation of mesostructured polyoxometalates/copolymers nanocomposites, which can be converted into metal oxides. This SPEA method enables the synthesis of functional ordered mesoporous metal oxides with different pore sizes, structures, compositions and tailored pore-wall microenvironments that are difficult to access via conventional direct organic-inorganic assembly. Typically, nitrogen-doped mesoporous ε-WO3 with high specific surface area, uniform mesopores and stable framework is obtained and exhibits great application potentials such as gas sensing.

Life cycle assessment and life cycle cost analysis of different walling materials with an environmental approach (comparison between earth-based vs. conventional construction techniques in Iran)

PurposeClimate change, environmental concerns, and economic problems pose challenges to the construction sector in Iran, which must provide affordable solutions while addressing environmental issues. Hence, natural earthen building materials are critically needed to reduce energy-intensive and costly construction practices dramatically. The purpose of this paper is to provide a framework for comparing life cycle assessments (LCA) and life cycle costs (LCC), for load-bearing walls of an single-family affordable housing unit in a desert part of Iran, Ardakan City.MethodsTo do so, both LCA and LCC for the unit were performed, considering a cradle-to-site perspective. For this purpose, 22 load bearing wall systems are assessed, including 18 stabilized and unstabilized earthen construction techniques, such as adobe, rammed earth (RE), and compressed earth block (CEB), in addition to four conventional wall assemblies of fired brick (FB), autoclaved aerated concrete block (AAC), ceramic block (CB), and concrete masonry unit (CMU). As well as assessing the environmental impact and life cycle costs associated with the life cycle of each wall, the optimal assembly of the wall is also examined.ResultsResults show that unstabilized earthen walling alternatives have significantly lower environmental impacts than conventional materials.ConclusionsSensitivity analysis indicates that by utilizing local materials to the maximum extent possible, impacts can be further minimized. Considering the results, transportation may even account for a greater proportion of EI than wall components.

Human-robot collaboration in assembly line balancing problems: Review and research gaps

Researchers have been working on assembly line optimization problems, especially assembly line balancing, for decades. Integration of Industry 4.0 technologies into assembly lines creates new challenges in optimal resource selection, task allocation, line balance, and scheduling decisions, to name a few. Over the last decade, researchers have developed novel frameworks for conventional assembly line optimization problems that comply with the recent industrial revolution and leading technologies. State-of-the-art Balancing problems in assembly lines with collaborative robots are the core of this study as a key decision in Assembly 4.0. The collaboration of humans and robots in a fenceless environment requires a more comprehensive approach than the traditional task-station assignment in a balancing model. Resource selection/allocation and scheduling are the most common decisions that researchers combine with balancing models. Thus, the studies on all these optimization challenges in the last ten years through the Web of Science and Engineering Village are present in the current review. We analyze the articles’ statistics (e.g., year and journals) and contents to find the research trends and gaps for future research direction in collaborative robotic assembly line optimization which incorporates occupational health and safety.

Opportunities for local electric vehicle manufacturing in Africa

In order to achieve the Sustainable Development Goals (SDG) of the United Nations on Clean Energy, Sustainable Industrialization and Climate Change, there is an urgent call on Africa to decarbonize transportation. Achieving these goals requires that Africa grows its current, largely, non-existent EV fleet to a reasonable level in the near future, to counter harmful emissions from fossil fuel vehicles. This paper discusses the opportunities available to African entrepreneurs on investing in electric vehicle (EV) manufacturing. It also discusses the factors militating against the establishment of a viable automotive sector. To exploit the opportunities offered despite the limitations, the paper advocates a paradigm shift from the conventional vehicle assembly of foreign vehicles to one in which African original designs are made in low-cost, low-volume vehicle manufacturing plants consisting of general-purpose workshop equipment and intensive labour such that the whole value chain for vehicle manufacturing is developed.

Ultrasound-enhanced catalytic hairpin assembly capable of ultrasensitive microRNA biosensing for the early screening of Alzheimer's disease

Catalytic hairpin assembly (CHA) is a promising enzyme-free, isothermal signal amplification strategy, but the relatively time-consuming strand replacement limits its application scenarios. Here, we developed an ultrasound-enhanced catalytic hairpin assembly (UECHA) biosensing platform for early screening of Alzheimer's disease by introducing a portable acoustic-drive platform with functionalized microspheres for effective biomarkers enrichment and fluorescence enhancement. By constructing a gradient ultrasonic field in a microcavity, the platform concentrates the functionalized microspheres in a central position, accompanied by an enhanced fluorescence signal with a specific release. In addition, the programmable frequency modulation can also modify the acoustic potential well and effectively promote non-equilibrium chemical reactions such as CHA (25 min). Compared with the conventional catalytic hairpin assembly (CHA), UECHA allows for direct and quantitative measurement of AD miRNAs down to 3.55 × 10−15 M in 1 μL samples. This visual analysis of ultra-trace biomarkers based on acoustic enrichment and promotion provides a new perspective for the rapid and highly sensitive clinical detection of Alzheimer's disease.

Base-Stacking-Driven Catalytic Hairpin Assembly: A Nucleic Acid Amplification Reaction Using Electrode Interface as a "Booster" for SARS-CoV-2 Point-of-Care Testing.

Electrochemical DNA (E-DNA) biosensors based on interface-mediated hybridization reactions are promising for point-of-care testing (POCT). However, the low efficiency of target recycle amplification and the steric hindrance at the electrode interface limit their sensing performance. Herein, we propose a base-stacking-driven catalytic hairpin assembly (BDCHA), a nucleic acid amplification reaction strategy, for POCT. The introduction of the base-stacking effect in this strategy increases the thermodynamic stability of the product, thereby effectively improving the recycling efficiency. Also, it enables the interface-mediated hybridization to maintain stability with even fewer bases in the reaction-binding domain, hence minimizing DNA secondary structure formation or intertwining at the electrode surface and ameliorating the steric hindrance limitation. The introduced base-stacking effect makes the electrode serve as a "booster" by integrating the advantages of homogeneous and heterogeneous reactions, giving BDCHA an increased reaction rate of about 20-fold, compared to the conventional catalytic hairpin assembly. As a proof of concept, our BDCHA was applied in constructing a portable E-DNA biosensor for the detection of a SARS-CoV-2 N gene sequence fragment. A simple 30 min one-pot incubation is required, and the results can be readily read on a smartphone, making it portable and user-friendly for POCT.

Self-evolved BOx anchored on Mg2B2O5 crystallites for high-performance oxidative dehydrogenation of propane

Oxidative dehydrogenation of propane (ODHP) is a promising process for producing propene. Recently, some boron-based catalysts have exhibited excellent olefin selectivity in ODHP. However, their complex synthetic routes and poor stability under high-temperature reaction conditions have hindered their practical application. Herein, we report a self-evolution method rather than conventional assembly approaches to acquire structures with excellent stability under a high propane conversion, from a single precursor-MgB2. The catalyst feasibly prepared and optimized exhibited a striking performance: 60% propane conversion with a 43.2% olefin yield at 535°C. The BOx corona pinned by the strong interaction with the borate enabled zero loss of the high conversion (around 40%) and olefins selectivity (above 80%) for over 100h at 520°C. This all-in-one strategy of deriving all the necessary components from just one raw chemical provides a new way to synthesize effective and economic catalysts for potential industrial implementation.

Pressure-Responsive Polymer Chemosensors for Hydrostatic-Pressure-Signal Detection: Poly-l-Lysine-Pyrene Conjugates.

Stimulus-responsive polymer materials are an attractive alternative to conventional supramolecular and polymer assemblies for applications in sensing, imaging, and drug-delivery systems. Herein, we synthesized a series of pyrene-labeled α- and ε-poly-l-lysine conjugates with varying degrees of substitution (DSs). Hydrostatic-pressure-UV/vis, fluorescence, and excitation spectroscopies and fluorescence lifetime measurements revealed ground-state conformers and excited-state ensembles emitting fluorescence with variable intensities. The polylysine-based chemosensors demonstrated diverse ratiometric responses to hydrostatic pressure through adjustments in polar solvents, DSs, and polymer backbones. Additionally, the fluorescence chemosensor exhibited a promising glum value of 3.2 × 10-3, indicating potential applications in chiral fluorescent materials. This study offers valuable insights into the development of smart hydrostatic-pressure-responsive polymer materials.