Controlled Water Deficit Research Articles

Biostimulators with marine algae extracts and their role in increasing tolerance to drought stress in highbush blueberry cultivation

Drought is one of the most serious challenges facing agriculture and ecosystems around the world. With more frequent and more extreme weather events, the effects of drought are becoming more severe, leading to yield losses, soil depletion and environmental degradation. In this work, we present an analysis of the impact of a marine algae biostimulanat andits ability to offset the effects of drought stress in blueberry cultivation. The aim of the research was to evaluate various fertilisation programs in increasing plant resistance to abiotic stress such as drought. It was tested whether the algal biostimulator provides the same tolerance to drought stress in highbush blueberry plants as regular fertilisers without biostimulation. The research was conducted in 2022 in a greenhouse in controlled drought conditions. Three-year-old highbush blueberry bushes (12 pieces) were used in the experiment. Highbush blueberry bushes (Vaccinium corymbosum) ’Brigitta Blue’ varieties were planted in plastic pots with a capacity of 10 dm3 containing an acidic substrate and placed in a greenhouse. Controlled lighting conditions were maintained using sodium lamps and a temperature of 25°C/20°C day/night. The substrate in pots was maintained at 80% of field water capacity by manual watering and weekly supply of nutrient solution for 5 weeks until water deficit occurred. Half of the plants were sprayed weekly with biostimulant at a concentration of 1%, three times 1 week apart (1 application per week). The biostimulant was evenly applied to the entire plant. Seven days after the third application of the product, half of the unsprayed and sprayed plants were subjected to water deficit stress by holding thewatering until 40% of the field water capacity (FC) was reached. The experimental layout included four combinations: C—Control—no biostimulation, no water deficit; CS—Stress control—water deficit up to 40% FC, no biostimulation; B—Biostimulator—no water deficit, biostimulation; BS—Stress plus biostimulator—water deficit up to 40% FC, biostimulation. Fertilisers with seaweed extracts show the ability to reduce the adverse effects of stress, promoting plant resilience, including tolerance to drought stress. The following were evaluated in the experiment: catalase activity, peroxidase activity, free malondialdehyde content, photosynthetic activity and leaf mineral content. The biostimulant used in experiment increased the oxidative activity of the enzymes pe-roxidase and catalase under simulated drought stress conditions. The algal biostimulant increased the average value of catalase activity by 20% in comparison to the control plants, in both combinatinations. The tested biostimulator had no effect on the chlorophyll content in the leaves or the concentration of nutrients in the leaves. The effect of marine algae products on the yield quantity and high quality is related among other to bioactive substances which helps to prevent drought stress.

Drought and mycorrhizal inoculation affect growth and morphology of Australian stormwater biofilter plants

This study evaluated the effect of mycorrhizal inoculation on six common Australian biofilter plant species using a pot experiment. We divided 120 pots planted with six common biofilter species into four treatments: control water-deficit, inoculated water-deficit, control well-watered, and inoculated well-watered. Inoculated treatments were inoculated with a commercially available blend of arbuscular mycorrhizal fungi spores. While well-watered pots were watered to pot capacity daily, water-deficit pots were subjected to a simulated drought that lasted six weeks until harvesting. All plants inoculated with arbuscular mycorrhizal fungi developed greater total root surface area, plant biomass, and plant relative growth rate. Which are associated with better stormwater nutrient removal. Mycorrhizal inoculation also increased the average root diameter, total length, and surface area of thick roots (diameter > 1 mm) of six species, which are essential to maintain the infiltration rates and hydraulic functions of biofilter systems. Moreover, an increase in shoot biomass following inoculation was also observed across six species, potentially contributing to higher water loss and stormwater volume reduction. Although the water-deficit condition greatly limited the effects of mycorrhizal inoculation on other morphological traits, we still observed the benefits of inoculation on total root length and surface area in well-watered J. pallidus and C. appressa. As drought stress imposes limitations on the effects of inoculation, we recommend inoculation should be carried out in advantageous times, such as during wet periods before the drought season for the optimal colonization and plant biomass and morphological responses. Additionally, selecting mycorrhizae-responsive species like C. appressa, J. pallidus, and G. sieberiana can result in favorable outcomes.

Production and economic viability of ‘Kent’ mango subjected to regulated deficit irrigation in the semi-arid

Mango (Mangifera indica L.) production has great importance for Brazilian agriculture, being concentrated in the states of Bahia and Pernambuco. Although mango is a drought-tolerant crop, inadequate irrigation management reduces its yield. Thus, the objective was to evaluate production parameters and economic viability of ‘Kent’ mango subjected to controlled water deficit in the phenological stages of flowering, fruit growth and maturation. The experiment was conducted in the municipality of Petrolina/PE, semi-arid region of the São Francisco Valley, in a ultisol. The experimental design was randomized blocks, in a triple factorial scheme, with application of four irrigation depths (40, 60, 80 and 100% of crop evapotranspiration - ETc), three phenological stages (F1 - flowering, F2 - fruit growth, F3 - fruit maturation) and two production cycles (2018 and 2019 seasons), with four replicates, each of which consisting of four plants. The irrigation with 40% ETc in the flowering stage and 80% of the ETc in the growing and ripening of fruits phases promoted higher yield, water use efficiency and benefit/cost ratio in the cultivation of ‘Kent’ mango in the Brazilian semi-arid region.

Ploidy's Role in Daylily Plant Resilience to Drought Stress Challenges.

This study aimed to understand the differences in the performance of diploid and tetraploid daylily cultivars under water deficit conditions, which are essential indicators of drought tolerance. This research revealed that tetraploid daylilies performed better than diploid varieties in arid conditions due to their enhanced adaptability and resilience to water deficit conditions. The analysis of the results highlighted the need to clarify the specific physiological and molecular mechanisms underlying the enhanced drought tolerance observed in tetraploid plants compared to diploids. This research offers valuable knowledge for improving crop resilience and sustainable floricultural practices in changing environmental conditions. The morphological and physiological parameters were analyzed in 19 diploid and 21 tetraploid daylily cultivars under controlled water deficit conditions, and three drought resistance groups were formed based on the clustering of these parameters. In a high drought resistance cluster, 93.3% tetraploid cultivars were exhibited. This study demonstrates the significance of ploidy in shaping plant responses to drought stress. It emphasizes the importance of studying plant responses to water deficit in landscape horticulture to develop drought-tolerant plants and ensure aspects of climate change.

New challenges for Uruguayan viticulture

Climate scenarios in the medium and long term (2010-2070) foresee increased summer rainfall for Uruguay and the region, with increased water deficits and excess episodes. Although at the international level irrigation in viticulture has a long experience and tradition, at the local level (Uruguay), only 10% of the vineyard surface area implements a fixed or complementary system for water supply in their crops. This work aimed to model the crop water requirements for a vineyard in southern Uruguay based on pedo-climatic variables. In addition, the plant response to controlled deficit irrigation was evaluated in two consecutive seasons. The experiment was conducted in a 1.1 ha commercial vineyard in Canelones, Uruguay (34°36'S, 56°14W), during two successive seasons (2020-2021). The additional irrigation (I) treatment was compared against a control (C) without irrigation. A controlled water deficit was established from flowering to harvest. The adjustment in the demand was made as a function of a percentage of crop evapotranspiration. The Kc of the crop was estimated using digital tools. The simulation of the water balance made it possible to evaluate the vineyard water needs. Plants subjected to controlled deficit irrigation showed higher vegetative growth, positively impacting yield and the accumulation of sugars and anthocyanins in the berry. Based on our results, a supplementary water supply, at the right doses and time, allows us to face water deficit situations, positively impacting the productive and economic variables. Knowing the variability in a vineyard is necessary to achieve proper irrigation scheduling and optimize water use. New technologies applied to irrigation are an opportunity for winegrowers to obtain more sustainable vineyards and production.

Irrigation Strategies with Controlled Water Deficit in Two Production Cycles of Cotton.

Water scarcity is one of the main abiotic factors that limit agricultural production. In this sense, the identification of genotypes tolerant to water deficit associated with irrigation management strategies is extremely important. In this context, the objective of this study was to evaluate the morphology, production, water consumption, and water use efficiency of colored fiber cotton genotypes submitted to irrigation strategies with a water deficit in the phenological phases. Two experiments were conducted in succession. In the first experiment, a randomized block design was used in a 3 × 7 factorial scheme, corresponding to three colored cotton genotypes (BRS Rubi, BRS Jade, and BRS Safira) in seven irrigation management strategies with 40% of the real evapotranspiration (ETr) varying the phenological stages. In the second experiment, the same design was used in a 3 × 10 factorial arrangement (genotypes × irrigation management strategies). The water deficit in the vegetative phase can be used in the first year of cotton cultivation. Among the genotypes, 'BRS Jade' is the most tolerant to water deficit in terms of phytomass accumulation and fiber production.

Exogenous β-Aminobutyric Acid (BABA) Improves the Growth, Essential Oil Content, and Composition of Grapefruit Mint (Mentha suaveolens × piperita) under Water Deficit Stress Conditions

Grapefruit mint, Mentha suaveolens × M. piperita, belonging to the Lamiaceae family, is an important medicinal plant with applications in the cosmetic, pharmaceutical, food, and nutraceutical industries. Environmental factors such as cold, salinity, and water deficit significantly impact the quantity and quality of the active compounds of medicinal plants. To examine the effects of drought stress and β-aminobutyric acid (BABA) as an elicitor on the biochemical characteristics and essential oil (EO) profile of grapefruit mint, a factorial experiment was conducted in a completely randomized design (CRD) with two factor and three replications under greenhouse conditions. The first factor included field moisture capacity (FC) as the control (100% FC), mild (75% FC), moderate (55% FC), and severe water deficit stress (35% FC), while the second factor consisted of 0 (control plants without BABA), 0.8, 1.6, and 2.4 mM of BABA foliar application. Water stress and BABA application significantly affected the EO content and composition of grapefruit mint. The highest content of EO was observed in mild drought stress and BABA spraying at 1.6 to 2.4 mM, which increased by about 140% compared with the control condition. The EO components were identified using GC-FID and GC-MS analysis. Linalool (33.7–47.3%) and linalool acetate (31.2–52%) were the most abundant compounds. The highest content of linalool acetate was observed in severe drought stress (35% FC) with foliar application of BABA (1.6 mM), which increased by 33.86% compared with the control condition. However, the highest content of linalool was observed under normal irrigation with foliar application of 0.8 to 1.6 mM BABA. Based on the results, severe drought stress reduced the total chlorophyll and carotenoids by 81.76 and 64.6% compared with the control condition, respectively. Water stress and the foliar application of BABA significantly affected the activity of antioxidant enzymes (ascorbate peroxidase, APX; guaiacol peroxides, GPX; and superoxide dismutase, SOD). The application of 1.6 mM BABA significantly increased the activity of antioxidant enzymes under water stress conditions. Finally, our results showed that the application of BABA (mainly at 1.6 mM) can improve the grapefruit mint yield and EO profile under water stress conditions.

Gas exchange and post-harvest quality of ‘Kent’ mango subjected to controlled water deficit in semi-arid region

ABSTRACT Brazil is the seventh largest producer of mangoes in the world, and the São Francisco Valley is the main producing region, with ‘Kent’ as one of the main cultivars. Considering that irrigation management affects physiological events, production and fruit quality, the objective of this work was to evaluate the gas exchange in the leaves and fruit quality of ‘Kent’ mango subjected to controlled water deficit in the semi-arid region. The experiment was carried out in Petrolina-PE, semi-arid region of the São Francisco Valley. The experimental design used was randomized blocks, in a triple factorial scheme, with four irrigation depths (40, 60, 80 and 100% of crop evapotranspiration - ETc), three phenological stages (F1 - flowering, F2 - fruit growth, F3 - fruit maturation) and two production cycles (2018 and 2019), with four replicates. During the phenological stages, the physiological parameters of gas exchange were evaluated. After harvesting, the following parameters were evaluated: soluble solids content, titratable acidity, pH, firmness and peel color. Irrigation with a depth between 79.5 and 83.6% ETc during the evaluated stages promoted greater gas exchange in the leaves, greater firmness and acidity in the fruits. The reduction in irrigation depth in F1 and F3 reduced the soluble solids content, while in F2 the highest soluble solids content was obtained with irrigation between 68.24 and 74.5% ETc. The most suitable irrigation depth for ‘Kent’ mango cultivation depends on the purpose of the producer and on the phenological stage of the crop.

Irrigation strategies in production of cherry tomatoes under water scarcity conditions

ABSTRACT Controlled water deficit in the phenological phases of cherry tomato can be used without significantly impairing crop yield. The objective was to determine the coefficients of sensitivity to water deficit in cherry tomatoes and to understand the effects of this deficit in agronomic variables and water use efficiency for different irrigation strategies based on phenological stages. The experimental design used was randomized blocks in a split-plot scheme, with four replicates. The primary treatments distributed in the plots were as follows: control without induction of water deficit (W1) and induction of water deficit in the vegetative stage (W2), in the flowering stage (W3), in the fruiting stage (W4), in the maturation stage (W5), and in all phenological stages (W6). The secondary treatments comprised two cherry tomato cultivars, orange and red, placed in the subplots. Water deficit was established at 50% of crop evapotranspiration. Cherry tomato is recommended for production in semiarid regions owing to their adaptability to controlled water deficit based on the coefficient of sensitivity to water deficit, which assists in the management of water resources for irrigation and the maintenance of crop productivity. The vegetative stage is recommended for the deficit irrigation strategy. However, water deficit should not be implemented in the flowering stage, which is considered the most critical stage for the application of water deficit in both cultivars.

Early selection of drought stress tolerance in potato genotypes using polyethylene glycol (PEG) in the in-vitro condition

Drought stress threatens potato production by reducing tuber yield. To maintain the supply of potatoes, potato cultivars that are able to adapt and tolerate to water deficit conditions are needed. The aim of this study was to obtain potato genotypes with high tolerance in the early in-vitro selection by Polyethylene Glycol (PEG) treatment. Fifty-one potato genotypes were used in this in-vitro evaluation, cultured in the MS medium which treated by four level of PEG 6000 to induce the drought stress, namely 0, 30, and 60 g.L-1. The study revealed that in-vitro plants respond to PEG-induced drought stress by reducing their growth and vigour. The decreasing of plantlet vigour and leaves size also observed. At the end of observation, five genotypes performed good vigour at the 60 g.L-1 of PEG 6000, namely CIP 394613.139 × 5.13:1.1, ATL × 3.11:1.7, 2.13 × ATL:1.12.10.3, ATL × 3.11:3.6, and ATL × 3.11:8.14. Those genotypes were indicated tolerant to drought stress and need to be followed up with further evaluation in the green house environment to see the ability of tuberization under controlled water deficit condition.

High-throughput phenotyping of physiological traits for wheat resilience to high temperature and drought stress.

Interannual and local fluctuations in wheat crop yield are mostly explained by abiotic constraints. Heatwaves and drought, which are among the top stressors, commonly co-occur, and their frequency is increasing with global climate change. High-throughput methods were optimized to phenotype wheat plants under controlled water deficit and high temperature, with the aim to identify phenotypic traits conferring adaptative stress responses. Wheat plants of 10 genotypes were grown in a fully automated plant facility under 25/18 °C day/night for 30 d, and then the temperature was increased for 7 d (38/31 °C day/night) while maintaining half of the plants well irrigated and half at 30% field capacity. Thermal and multispectral images and pot weights were registered twice daily. At the end of the experiment, key metabolites and enzyme activities from carbohydrate and antioxidant metabolism were quantified. Regression machine learning models were successfully established to predict plant biomass using image-extracted parameters. Evapotranspiration traits expressed significant genotype–environment interactions (G×E) when acclimatization to stress was continuously monitored. Consequently, transpiration efficiency was essential to maintain the balance between water-saving strategies and biomass production in wheat under water deficit and high temperature. Stress tolerance included changes in carbohydrate metabolism, particularly in the sucrolytic and glycolytic pathways, and in antioxidant metabolism. The observed genetic differences in sensitivity to high temperature and water deficit can be exploited in breeding programmes to improve wheat resilience to climate change.

Photoprotection Is Achieved by Photorespiration and Modification of the Leaf Incident Light, and Their Extent Is Modulated by the Stomatal Sensitivity to Water Deficit in Grapevines.

Absorbed energy in excess of that used by photosynthesis induces photoinhibition, which is common in water deficit conditions, resulting in reductions in stomatal conductance. In grapevines, controlled water deficit is a common field practice, but little is known about the impact of a given water shortage on the energy transduction processes at the leaf level in relation to contrasting stomatal sensitivities to drought. Here, we assessed the effect of a nearly similar water deficit condition on four grapevine varieties: Cabernet Sauvignon (CS) and Sauvignon Blanc (SB), which are stomatal sensitive, and Chardonnay (CH) and Carménère (CM), which are less stomatal sensitive, grown in 20 L pots outdoors. Plants were maintained to nearly 94% of field capacity (WW) and 83% field capacity (WD). We have assessed plant water status, photosynthesis (AN), photorespiration, AN vs. PAR, ACi curves, photochemical (qP) and non-photochemical (qN) fluorescence quenching vs. PAR, the photoprotective effectiveness of NPQ (qPd) and light interception by leaves. Photorespiration is important under WD, but to a different extent between varieties. This is related to stomatal sensitivity, maintaining a safe proportion of PSII reaction centres in an open state. Additionally, the capacity for carboxylation is affected by WD, but to a greater extent in more sensitive varieties. As for qN, in WD it saturates at 750 μmol PAR m−2s−1, irrespective of the variety, which coincides with PAR, from which qN photoprotective effectiveness declines, and qP is reduced to risky thresholds. Additionally, that same PAR intensity is intercepted by WD leaves from highly stomatal-sensitive varieties, likely due to a modification of the leaf angle in those plants. Pigments associated with qN, as well as chlorophylls, do not seem to be a relevant physiological target for acclimation.

Adjustments in photosynthesis and leaf water relations are related to changes in cell wall composition in Hordeum vulgare and Triticum aestivum subjected to water deficit stress

In the current climate change scenario, understanding crops’ physiological performance under water shortage is crucial to overcome drought periods. Although the implication of leaf water relations maintaining leaf turgor and stomatal functioning under water deprivation has been suggested, the relationships between photosynthesis and osmotic and elastic adjustments remain misunderstood. Similarly, only few studies in dicotyledonous analysed how changes in cell wall composition affected photosynthesis and leaf water relations under drought. To induce modifications in photosynthesis, leaf water relations and cell wall composition, Hordeum vulgare and Triticum aestivum were subjected to different water regimes: control (CL, full irrigation), moderate and severe water deficit stress (Mod WS and Sev WS, respectively). Water shortage decreased photosynthesis mainly due to stomatal conductance (gs) declines, being accompanied by reduced osmotic potential at full turgor (πo) and increased bulk modulus of elasticity (ε). Whereas both species enhanced pectins when intensifying water deprivation, species-dependent adjustments occurred for cellulose and hemicelluloses. From these results, we showed that πo and ε influenced photosynthesis, particularly, gs. Furthermore, the (Cellulose+Hemicelluloses)/Pectins ratio determined ε and mesophyll conductance (gm) in grasses, presenting the lowest pectins content within angiosperms. Thus, we highlight the relevance of cell wall composition regulating grasses physiology during drought acclimation.

Effects of Soil Water Deficit on Three Tree Species of the Arid Environment: Variations in Growth, Physiology, and Antioxidant Enzyme Activities

Low water availability predicted under climate change is a major abiotic factor limiting plants growth and productivity. In this study a greenhouse experiment was conducted on three important tree species of arid environment: Conocarpus erectus (CE), Acacia modesta (AM), and Salix tetrasperma (ST). Young saplings were subjected to control (C), medium (MWD) and severe soil water deficit (SWD) treatments and response was evaluated. Results showed that in all the three species leaf, stem and root dry weight production remained similar to C under MWD treatment but decreased significantly under SWD. The highest decrease in total dry weight was noticed in ST and the lowest was evidenced in AM under SWD. Root:shoot ratio increased significantly in both CE and AM under MWD and SWD. Furthermore, chlorophyll content decreased while proline content increased significantly in both MWD and SWD treatments. The production of oxidants (hydrogen peroxide and superoxide anions) and antioxidants (superoxide dismutase, catalase, peroxidase and ascorbate peroxidase) increased significantly under both MWD and SWD treatments and were the highest in AM in both MWD and SWD treatments. Therefore, we may conclude that all the three species can tolerate medium water stress due to increased root production and an effective antioxidant defense mechanism.

From grape berries to wines: drought impacts on key secondary metabolites

Aim. We aimed to study the impact of water deficit on the concentration of key flavour and phenolic secondary metabolites of wines.Methods and results. A drought-stress field trial was conducted on Vitis vinifera cv. Merlot and Tocai Friulano for two seasons. Fully irrigated (C) and deficit irrigated (D) grapes were microvinified and the resulting wines were analysed to determine the concentrations of anthocyanins, tannins, and free and glycosidically-bound Volatile Organic Compounds (VOCs). A descriptive sensory test was undertaken on the same wines. Water stressed grapes produced wines with higher concentrations of anthocyanins in Merlot and of free and glycosidically-bound monoterpenes in Tocai Friulano. Both cultivars displayed higher amounts of glycosidically-bound C13-norisoprenoids.Conclusions. Previously observed drought-induced compositional changes to the grapes were transfered to the wines, with an increase in polyphenols and VOCs. However, the timing and the duration of the water stress in the field only heavily impacted the final wine composition with major metabolic modification when the severe water deficit started early (at approximately 40 days after anthesis) and lasted over the entire season until harvest.Significance and impact of the study. This study highlights the positive role of a controlled water deficit on the composition of the wines in terms of secondary metabolites.

Transgenerational stress memory of water deficit in peanut production

To address a changing climate and dwindling water resources, many farmers have begun to adopt water-saving irrigation management practices in crop production. Consequently, the impacts of these water-management schemes on crop physiology and yield have been quantified within a single growing season, but relatively little work has been done in characterizing the effects of controlled water deficit on crop performance in the subsequent generation. Therefore, the major aim of this project was to explore the phenotypic impacts of water-saving irrigation management between generations and identify potential genotypic variation in these responses through the quantification of transgenerational stress memory (TSM) in the establishment, photosynthetic and agronomic performance of five genotypes of peanut. To address this objective, seeds were collected from parents that received two water regimes representing two parent stress histories with contrasting potential to induce TSM and providing two progeny types: 1) offspring of non-stressed parents and 2) offspring of stressed parents. Measurements of seedling emergence, photosynthetic functioning, and yield in three consecutive years indicated that TSM may exist in peanut. However, the ways in which TSM is expressed are complex and appear to be dependent on both the genotype and the immediate environmental conditions experienced by the offspring of stressed parents. The offspring of stressed parents had greater yield than the offspring of non-stressed parents, but only in one year of the study. On the other hand, field emergence was improved in the offspring of stressed parents in all years, indicating the stand establishment of peanut plants was determined, at least in part, by the water deficit incurred during the parent generation. These results suggest that the influence of TSM on peanut phenotypes was limited to the early stages of growth of field-grown peanut. Results of this study also highlight the need to further examine the role of management in the parental generation in determining peanut seed quality and subsequent plant performance under a variety of environmental conditions.

Controversies in Midday Water Potential Regulation and Stomatal Behavior Might Result From the Environment, Genotype, and/or Rootstock: Evidence From Carménère and Syrah Grapevine Varieties.

Controversies exist regarding the iso/anisohydric continuum for classifying plant water-use strategies. Isohydricity has been argued to result from plant–environment interaction rather than it being an intrinsic property of the plant itself. Discrepancies remain regarding the degree of isohydricity (σ) of plants and their threshold for physiological responses and resistance to drought. Thus, the aim of this study was to evaluate the isohydricity of the grapevine varieties Syrah and Carménère under a non-lethal water deficit progression from veraison from two different locations, the Cachapoal Valley (CV) and Maipo Valley (MV), in central Chile and with different rootstock only in Syrah. For this purpose, the midday stem water potential (Ψmds) regulation and stomatal responses to drought, leaf traits related to pressure–volume curves, stomatal sensitivity to ABA, cavitation threshold, and photosynthetic responses were assessed. A higher atmospheric water demand was observed in the CV compared to the MV, with lower Ψmds values in the former for both varieties. Also, the σ values in Carménère were 1.11 ± 0.14 MPa MPa-1 and 0.68 ± 0.18 MPa MPa-1 in the CV and MV, respectively, and in Syrah they were 1.10 ± 0.07 MPa MPa-1 in the CV and 0.60 ± 0.10 MPa MPa-1 in the MV. Even though similar variations in σ between locations in both varieties were evident, Carménère plants showed a conserved stomatal response to Ψmds in both study sites, while those of Syrah resulted in a higher stomatal sensitivity to Ψmds in the site of lower σ. Besides the differences in seasonal weather conditions, it is likely that the different rootstock and clonal variability of each season in Syrah were able to induce coordinated changes in σ, Ψgs12, and osmotic potential at full turgor (π0). On the other hand, irrespective of the σ, and given the similarity between the π0 and Ψgs12 in leaves before drought, it seems that π0 could be a convenient tool for assessing the Ψmds threshold values posing a risk to the plants in order to aid the irrigation decision making in grapevines under controlled water deficit. Finally, water deficits in vineyards might irreversibly compromise the photosynthetic capacity of leaves.

A coupled agent-based risk-based optimization model for integrated urban water management

Integrated urban water management (IUWM) is a comprehensive mitigation strategy to control water deficit due to simultaneous effects of reduction in water resources capacity and increase in water demand. Here we develop a coupled agent-based risk-based optimization model, which is able to account for water resources capacity and water demand uncertainties to determine the optimum annual water allocation. An optimization model based on conditional value at risk (CVaR) is employed to minimize the water supply risk in a long-term horizon and obtain the annual probabilities of complete supply of water demand. According to the generated synthetic weather data by LARS-WG for different climate scenarios, surface water and groundwater resources are modeled using HMETS and MODFLOW, respectively. Then, the developed agent-based model considers agents’ attributes, action roles and interactions by applying Borda scoring approach, and determines the most compatible IUWM strategies to increase water supply or modify water demand. The proposed framework is implemented for the Shiraz city in Iran, revealing a strong need to IUWM strategies to alleviate the water deficit over the period of 2018 to 2050. The proposed approach provides an efficient framework to achieve sustainable management strategies for cities with stressed water resources and rapid urban development.

Restoring a Natural Fire Regime Alters the Water Balance of a Sierra Nevada Catchment

AbstractFire suppression in western U.S. mountains has caused dense forests with high water demands to grow. Restoring natural wildfire regimes to these forests could affect hydrology by changing vegetation composition and structure, but the specific effects on water balance are unknown. Mountain watersheds supply water to much of the western United States, so understanding the relationship between fire regime and water yield is essential to inform management. We used a distributed hydrological model to quantify hydrologic response to a restored fire regime in the Illilouette Creek Basin (ICB) within Yosemite National Park, California. Over the past 45 years, as successive fires reduced the ICB's forest cover approximately 25%, model results show that annual streamflow, subsurface water storage, and peak snowpack increased relative to a fire‐suppressed control, while evapotranspiration and climatic water deficit decreased. A second model experiment compared the water balance in the ICB under two vegetation cover scenarios: 2012 vegetation, representing a frequent‐fire landscape, and 1969 vegetation, representing fire suppression. These two model landscapes were run with observed weather data from 1972 to 2017 in order to capture natural variations in precipitation and temperature. This experiment showed that wet years experienced greater fire‐related reductions in evapotranspiration and increases in streamflow, while reductions in climatic water deficit were greater in dry years. Spring snowmelt runoff was higher under burned conditions, while summer baseflow was relatively unaffected. Restoring wildfire to the fire‐suppressed ICB likely increased downstream water availability, shifted streamflows slightly earlier, and reduced water stress to forests.

Influence of deficit irrigation on accumulation and partitioning of sugarcane biomass under drip irrigation in commercial varieties

Studies of sugarcane varieties grown under water-restrictive conditions are necessary because climate changes may result in insufficient rain for agriculture and selecting or breeding drought-tolerant cultivars will become more and more important. This study aimed to quantify the accumulation and partitioning of biomass, as well as water use efficiency of commercial sugarcane varieties under different soil water availability conditions throughout the growing season. The study was conducted during two cropping cycles in Southeast Brazil (22.7 °S, 47.6 °W). Four soil moisture availability regimens were set up to provide 100, 75, 75* and 50% of the water demand of eight commercial varieties of sugarcane (CTC15, CTC17, RB867515, RB92579, RB931011, RB966928, IAC5000, and NCo376). The total aboveground biomass was measured in both crop cycles. Our results indicate that two Brazilian varieties (CTC15 and CTC17) and one South African variety (NCo376 - Canegrow DSSAT reference), increased their water use efficiency when grown under an irrigation deficit of 75% of normal, without yielding less biomass. We did not observe any change in sucrose content under controlled water deficits; however, the studied varieties showed different biomass partitioning: RB966928 had the largest biomass fraction allocated in the stalks (0.59), while IAC5000 showed the lowest fraction (0.51). We verified that the harvest index for sucrose was not altered by reduced irrigation, but that its variability was attributed to the genotype inherited from traditional breeding programs. Thus, we conclude from these results that increased sugar yield per unit area is only possible nowadays by increasing sugarcane biomass productivity under highly efficient irrigation conditions to minimize the loss of yield from water stress.