Controlled Environment Room Research Articles

Bending performance of cross-laminated timber constructed from fibre-managed Eucalyptus nitens under short-term and long-term serviceability loads

AbstractCross-laminated timber (CLT) has become a notable building material due to its structural efficiency, reliability and sustainability. In this study, the bending performance of three-layered CLT constructed from fibre-managed Eucalyptus nitens (E. nitens) was investigated under short-term and long-term loadings. Linear-elastic four-point bending testing was used to determine the maximum serviceability loading capacity before they reached the suggested deflection limits. A pilot study was conducted to investigate the creep behaviour of E. nitens CLT through long-term bending tests in a controlled environmental room. The study suggested that E. nitens CLT has higher serviceability loading capacity and lower creep ratio compared to CLT made from strength-class C24 spruce. The investigations of various configurations of E. nitens CLT panels based on structural grades implemented in top, cross, and bottom layers have revealed different short-term and long-term bending performances. The grade of transverse layers has been found to be the most important factor in improving the bending creep performance of E. nitens CLT. Two modelling equations were employed to perform curve fitting on the experimental creep ratio with time. The conventional power-law modelling tends to underestimate the longer-term creep ratio when compared to a recently developed nonlinear regression modelling equation that takes environmental conditions into account. The mean estimated creep ratio after 50 years was 1.77 for E. nitens CLT, and 1.89 for the C24 spruce CLT. The present study is a pilot investigation to increase the understanding of performance of the newly developed CLT made from fibre-managed plantations E. nitens, with particular emphasis on its creep behaviour. The results of this study provide valuable contributions for future research in this field, and ongoing commercial production of E. nitens CLT.

UV-NDVI for real-time crop health monitoring in vertical farms

This study aims to develop a plant health monitoring system suitable for vertical farms. It began by creating a multispectral LED with UVA and NIR light sources and an IoT device capable of controlling the LED spectrum. The IoT device integrates a camera with switchable filters and RGB CMOS sensor to simultaneously calculate SI-NDVI (single-image normalized difference vegetation index) and UV-NDVI (UV induce red chlorophyll fluorescence normalized difference vegetation index).A lettuce cultivation experiment was conducted on a planting shelf in a controlled environmental room, with two cultivation planters containing the same nutrient solution and red lettuce variety. SI-NDVI and UV-NDVI were calculated every three hours to compare differences. Results showed that both UV-NDVI and SI-NDVI trends were similar in monitoring lettuce growth. However, UV-NDVI was more sensitive to plant health than SI-NDVI. By inducing drought stress, the UV-NDVI indicator was able to detect water deficiency anomalies earlier than visual observation.

Relationship Between Airborne Fungi Presence and the Position of the High Efficiency Particulate Air Filter in the Heating, Ventilation, and Air Conditioning System.

Establish the influence of the terminal or nonterminal position of High Efficiency Particulate Air (HEPA) filters in the Heating, Ventilation, and Air Conditioning (HVAC) system on the presence of airborne fungi in controlled environment rooms. Fungal infections are an important cause of morbidity and mortality in hospitalized patients. This study was realized from 2010 to 2017, in rooms with terminal and nonterminal HEPA filters, in eight Spanish hospitals. In rooms with terminal HEPA filters, 2,053 and 2,049 samples were recollected, and in rooms with nonterminal HEPA filters, 430 and 428 samples were recollected in the air discharge outlet (Point 1) and in the center of the room (Point 2), respectively. Temperature, relative humidity, air changes per hour, and differential pressure were recollected. Multivariable analysis showed higher odds ratio (OR) of airborne fungi presence when HEPA filters were in nonterminal position (OR: 6.78; 95% CI [3.77, 12.20]) in Point 1 and (OR: 4.43; 95% CI [2.65, 7.40]) in Point 2. Other parameters influenced airborne fungi presence, such as temperature (OR: 1.23; 95% CI [1.06, 1.41]) in Point 2 differential pressure (OR: 0.86; 95% CI [0.84, 0.90]) and (OR: 0.88; 95% CI [0.86, 0.91]) in Points 1 and 2, respectively. HEPA filter in terminal position of the HVAC system reduces the presence of airborne fungi. To decrease the presence of airborne fungi, adequate maintenance of the environmental and design parameters is necessary in addition to the terminal position of the HEPA filter.

Long coleoptile genotype and soil texture interactions determine establishment success and early growth parameters of wheat sown at depth

Long coleoptile (LC) - wheat genotypes sown deeper (> 10 cm) than current practice (< 5 cm) enables earlier deep sowing into subsurface soil moisture. The LC genotype shows promise to broaden sowing windows and remove reliance on the shifting seasonal break for crop establishment. The suitability of this sowing strategy to the highly variable soils of the southern Australian wheatbelt warrants investigation to identify suitable environments for potential establishment benefits. We conducted controlled environment room (CER) and field experiments using pairs of near-isogenic-lines (NILs) that differed genetically only for coleoptile length. The experiments investigated the suitability of deep sown (12 cm) LC wheats in soils with different texture (sand versus loam). The CER experiment identified that LC genotypes exhibited greater emergence and early seedling growth, producing greater shoot and root biomass, when deep sown (12 cm) in loam. While LC and short coleoptile (SC) genotypes produced similar shoot growth when sown at depth in sand, LC produced greater root biomass reflected in more seminal roots. In the field on sand, LC and SC had similar emergence and early shoot growth characteristics at all sowing depths (5, 8 and 10 cm). However, the heavy-textured, loam soil reduced seedling emergence and restricted leaf growth with deep sowing (12 cm) in the SC. Our results indicate that the LC trait was more critical for plant emergence and early growth parameters (shoot and root biomass) from depth in a heavier textured loam compared with a sand. By identifying environments that will likely benefit most from deep sown LC, we are one step closer to adapting crop production to shifting rainfall patterns in southern Australia.

Tenebrio molitor L. larvae inclusion in quail diet as a fat source: effects on performance, meat quality, and fatty acid composition

This study was conducted to determine the effects ofTenebrio molitor larvae inclusion in diets instead of soybean and fish oil on Japanese quail’s performance, carcass, and meat quality. A total of 180 7-day-old quails (Coturnix coturnix japonica) of both sexes were housed in battery packs in a controlled environment room. Chicks were randomly allocated to three treatment groups, each with 5 replicate pens of 12 chicks. The dietary treatments were as follows: (1) control (C) group (soybean meal-based diet); (2) fish oil supplemented diet (FO, 30 g/kg DM fish oil); and (3)T. molitor larvae diet (TML, 100 g/kg DM), respectively. No differences were found between daily weight gain and daily feed intake (P&gt;0.05). The final body weight was higher in quail-fed TML and lower in quailfed FO (P&lt;0.01). The feed conversion ratio was improved with the TML diet in quail (P&lt;0.05). Carcass weight was higher in the TML group than in the FO group (P&lt;0.05). No differences between treatment groups were found in liver, heart, and spleen percentages (P&gt;0.05); however, the proventriculus percentage was higher in FO than in the TML group. The chemical characteristics of breast meat were unaffected by dietary treatments (P&gt;0.05). The fatty acids (FA) were similar primarily in quail breast meat fed C and TML diets; however, polyunsaturated FA (PUFA) was lower in the TML diet compared to the C diet (P&gt;0.05). In the FO groups C14:0, C20:4 n6 and n-3 fatty acids (C18:3, C20:3, C20:5, C22:6), saturated FA, PUFA, and n-3/n-6 were increased, monounsaturated FA, PUFA n-6, and n-6/n-3 were decreased compared to the C and TML groups (P&lt;0.01).

Evaluation of the Results of a Periodic Environmental Biosecurity Assessment Program on Air Quality in Controlled Environment Rooms of Hospitals.

Determine the utility of the Periodic Environmental Biosecurity Assessment Program (PEBAP) in achieving clean air as measured by the number of colony-forming units (CFU) of fungi and bacteria in the air. There is no international consensus on the sampling frequency, the recommended limits for microorganisms in the air nor on the usefulness of routine microbiological air monitoring of hospitals. During the PEBAP, data were recollected between 2010 and 2017 in eight hospitals in southeast Spain. Air samples were collected in very high risk rooms (VHRRs) and high risk rooms (HRRs), unoccupied, using active sampling methods. Temperature, relative humidity, air changes per hour (ACH), and differential pressure were measured. When limits of CFU of opportunistic fungi and bacteria established in the PEBAP were exceeded, corrective measures were adopted. We found a reduction (p < .01) of percentage of air samples with fungi growth throughout the years of PEBAP in all rooms. Aspergillus was the most frequent opportunistic fungus. We found a high compliance of the standards of CFU of bacteria in HRR, and the percentage of compliance in VHRR was lower than in HRR in all years. Differences in environmental and design parameters were statistically significant (p < .05) between rooms, except for ACH. PEBAP resulted in a useful tool to maintain and improve air quality in hospitals. The control of environmental biosecurity requires a multidisciplinary approach from preventive medicine, engineering, and cleaning services. Aspergillus is the most frequent opportunistic fungus in southeast Spain.

Impact of guttation fluid from perennial ryegrass infected with different strains of Epichloe festucae var. lolii endophyte on Microctonus aethiopoides adult longevity

Perennial ryegrass (Lolium perenne L.) grows in association with a fungal endophyte Epichloe festucae var. lolii (Latch, Christensen &amp; Samuels) Bacon &amp; Schardl, which produces alkaloids that protect the grass against grazing by mammals and insects. These alkaloids are found in guttation fluid (xylem sap exuded from leaves through special structures known as hydathodes) and have the potential to impact on beneficial invertebrates in pastoral ecosystems. Newly emerged adults of the parasitoid Microctonus aethiopoides Loan (Hymenoptera: Braconidae) were supplied with guttation fluid from pot-grown ryegrasses infected with three different strains of endophyte (standard, AR37, AR1) or no endophyte collected at different times of the year, or water, sucrose solution or no liquid. Longevity was compared when individuals were held in separate vials in controlled environment room at 20oC with 16:8 h light:dark photoperiod. An enzymatic method was used to measure sugars in guttation fluid samples collected on three dates. Guttation fluid from endophyte-infected grasses was found to have no detrimental effect on M. aethiopoides longevity and to contain glucose and fructose. Guttation fluid from AR37-infected ryegrass collected in autumn increased insect longevity compared to water and fluid from standard-type infected ryegrass by 26% and 24% respectively. The lack of available food sources in New Zealand ryegrass-dominant pastures means that guttation fluid from AR37-infected ryegrass in autumn may contribute to M. aethiopoides efficacy as a biocontrol agent through enhanced longevity.

HVAC Control Systems for a Negative Air Pressure Isolation Room and Its Performance

The controlled environment room, called an isolation room, has become a must have for medical facilities, due to the spreading of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), to isolate the high risk infected patients. To avoid the transmission of the virus through airborne routes, guidelines were published by the government and the association. A medical facility must comply with this document for high-risk patient treatment. A full-scale N class isolation room was built at Syiah Kuala University to investigate the performance in terms of the controller, temperature, pressure, humidity, and energy consumption. The isolation room was equipped with a proper capacity heating, ventilating, and air conditioning (HVAC) system, which consisted of an air conditioning compressor and a negative pressure generator (NPG), and its installation was ensured to fulfil the guidelines. Since the current NPG was controlled manually, a computer-based control system was designed, implemented, and compared with the manual control. The results showed that the computer-based control outputs better stability of pressure and electric power. For that reason, a computer-based control was chosen in the real case. To investigate the performance of the isolation room, a 24 h experiment was carried out under different parameter setups. The results showed that improvement of the control strategy for temperature and humidity is still necessary. The energy consumption during the activation of the NPG for the recommended negative pressure was slightly different. An additional piece of equipment to absorb the heat from the exhaust air would be promising to improve the energy efficiency.

Acoustic conditioning of the neonatal incubator compartment: Improvement proposal.

The objective of this work focuses on the study of the main sources of noise associated with incubators and the acoustic effects that derive from them. The method that has been established is based on tests carried out under different operating conditions of the incubators. Noise levels are analyzed under different boundary conditions (Neonatal ICU and “Controlled environment rooms”). Under normal operating conditions, the levels inside the incubator are around 56 dB (A), values that exceed the maximum limits recommended by the American Academy of Pediatrics. The scope of this study is to evaluate the existing noise levels in the incubator and analyze possible design improvements. The study was carried out in the hospitals of Cádiz, Huelva, and Malaga.

Separating the effects of air and soil temperature on silver birch. Part I. Does soil temperature or resource competition determine the timing of root growth?

The aboveground parts of boreal forest trees mostly grow earlier, and the roots later, in the growing season. We aimed to experimentally test whether the extrinsic driver of soil temperature or the intrinsic driver (resource competition between plant parts) is a more important control for the root and shoot growth of silver birch (Betula pendula Roth) seedlings. Sixteen two-year-old seedlings were grown in controlled environment rooms for two simulated growing seasons (GS1, GS2). In GS1, all the seedlings were acclimatized under the same conditions, but in GS2, the soil temperature treatments were: (i) constant 10°C (Cool); (ii) constant 18°C (Warm); (iii) early growing season at 10°C, switched to 18°C later (Early Cool Late Warm, ECLW) and (iv) early growing season 18°C, switched to 10°C later (Early Warm Late Cool, EWLC). The treatments did not affect growth allocation between shoots and roots. Warm soil benefitted shoot elongation as it slowed down in EWLC and accelerated in ECLW after the soil temperature switch. However, whole-tree biomasses were similar to Cool and the seedlings grew largest in Warm. Phenology was not strongly affected by soil temperature, and root and shoot growth did not usually peak simultaneously. Short root mortality increased strongly in ECLW and decreased in EWLC after the soil temperature switch. Long root longevity was not significantly affected but long root growth ceased earliest in ECLW. Soil warming increased foliar nutrient contents. Growth dynamics were not solely driven by soil temperature, but resource competition also played a significant role. The study showed the importance of soil temperature for fine root dynamics not only through root growth but also via root mortality, as soil warming increased mortality even more than growth. Soil temperature has complex effects on tree and soil functioning, which further affects carbon dynamics in forest ecosystems that have a climate feedback.

Water Stress Impacts on Grapevines (Vitis vinifera L.) in Hot Environments: Physiological and Spectral Responses

The projected increase in temperature and water scarcity represents a challenge for winegrowers due to changing climatic conditions. Although heat and drought often occur concurrently in nature, there is still little known about the effects of water stress (WS) on grapevines in hot environments. This study aimed to assess whether the grapevine’s physiological and spectral responses to WS in hot environments differ from those expected under lower temperatures. Therefore, we propose an integrated approach to assess the physiological, thermal, and spectral response of two grapevine varieties (Vitis vinifera L.), Grenache and Shiraz, to WS in a hot environment. In a controlled environment room (CER), we imposed high-temperature conditions (TMIN 30 °C–TMAX 40 °C) and compared the performance of well-watered (WW) and WS-ed potted own-rooted Shiraz and Grenache grapevines (SH_WW, SH_WS, GR_WW, and GR_WS, respectively). We monitored the vines’ physiological, spectral, and thermal trends from the stress imposition to the recovery after re-watering. Then, we performed a correlation analysis between the physiological parameters and the spectral and thermal vegetation indices (VIs). Finally, we looked for the best-fitting models to predict the physiological parameters based on the spectral VIs. The results showed that GR_WS was more negatively impacted than SH_WS in terms of net photosynthesis (Pn, GR-WS = 1.14 μmol·CO2 m−2·s−1; SH-WS = 3.64 μmol·CO2 m−2·s−1), leaf transpiration rate (E, GR-WS = 1.02 mmol·H2O m−2·s−1; SH-WS = 1.75 mmol·H2O m−2·s−1), and stomatal conductance (gs, GR-WS = 0.04 mol·H2O m−2·s−1; SH-WS = 0.11 mol·H2O m−2·s−1). The intrinsic water-use efficiency (WUEi = Pn/gs) of GR_WS (26.04 μmol·CO2 mol−1 H2O) was lower than SH_WS (34.23 μmol·CO2 mol−1 H2O) and comparable to that of SH_WW (26.31 μmol·CO2 mol−1 H2O). SH_WS was not unaffected by water stress except for E. After stress, Pn, gs, and E of GR_WS did not recover, as they were significantly lower than the other treatments. The correlation analysis showed that the anthocyanin Gitelson (AntGitelson) and the green normalised difference vegetation index (GNDVI) had significant negative correlations with stem water potential (Ψstem), Pn, gs, and E and positive correlation with WUEi. In contrast, the photochemical reflectance index (PRI), the water index (WI), and the normalised difference infrared index (NDII) showed an opposite trend. Finally, the crop water stress (CWSI) had significant negative correlations with the Ψstem in both varieties. Our findings help unravel the behaviour of vines under WS in hot environments and suggest instrumental approaches to help the winegrowers managing abiotic stress.

A new non-human primate model of desiccating stress-induced dry eye disease

Dry eye disease (DED), a multifactorial ocular surface disease, is estimated to affect up to 34% of individuals over 50 years old. Although numerous animal models, including rodents and rabbits, have been developed to mimic the pathophysiologic mechanisms involved in dry eye, there is a lack of non-human primate (NHP) models, critical for translational drug studies. Here, we developed a novel desiccating stress-induced dry eye disease model using Rhesus macaque monkeys. The monkeys were housed in a controlled environment room for 21 to 36 days under humidity, temperature, and airflow regulation. Following desiccating stress, NHPs demonstrated clinical symptoms similar to those of humans, as shown by increased corneal fluorescein staining (CFS) and decreased tear-film breakup time (TFBUT). Moreover, corticosteroid treatment significantly reduced CFS scoring, restored TFBUT, and prevented upregulation of tear proinflammatory cytokines as observed in dry eye patients following steroid treatment. The close resemblance of clinical symptoms and treatment responses to those of human DED patients provides great translational value to the NHP model, which could serve as a clinically relevant animal model to study the efficacy of new potential treatments for DED.

Self-Compacting Earth-Based Composites: Mixture Design and Multi-Performance Characterisation

A new generation of self-compacting earth-based composites (SCEC) for vertical elements with fully exposed earth, in which the main feature is the elimination of compaction, is proposed in the current work. The first objective was to develop earth-based mixture compositions with locally available raw materials and achieve self-compactability. A Portuguese soil, typically employed in rammed earth construction, was stabilised using cementitious materials and a superplasticiser to ensure flowability. The mixture design studies allowed to achieve flowable fresh composites. Results on the hardened state showed that SCEC presents high compressive strengths (5–14 MPa). The electrical resistivity SCEC increased with time and achieved at least 5600 Ωm at 28 days, while mass loss stabilised after 14 days in a controlled environment room. Additional tests were performed on selected optimal SCEC mixtures: drying shrinkage and water capillary absorption, with promising results. Overall, this work provided initial boundaries for the mixture design and assessment of SCEC and opened the door for further studies on new and novel earth materials construction techniques.

Prediction of the Energy Consumption for Indoor Strawberry Cultivation in a Tropical Climate

This article addresses the lack of information for predicting the energy consumption of strawberry plantations inside plant factories located in tropical climate regions. This study aims to investigate the energy consumption of the cultivation of strawberries in the controlled environment room and to develop a TRNSYS computer model for the controlled environment room. Experiments were conducted in a 25 m3 controlled environment room. There are 180 strawberry trees inside the room. Light Emitting Diode (LED) grow light substitutes for natural sunlight. An air conditioner was used to regulate the indoor air condition. A computer model was developed using TRNSYS (TRaNsientSYStem simulation tool) and was validated using the collected data. There are three main components of the room heat load: transmission, lighting, and evapotranspiration. The lighting heat load shares more than 96% of the total heat load — the evapotranspiration load increases when the LED turns on. However, the lighting consumes only about 36% of total electricity consumption, while the air conditioner consumes 64%. Most of the electricity is used during the runner stage. Electricity consumption can be saved by 40% if the runners are grown outside the plant factory. Therefore, the high heat load is a feature in the plant factory. In this study, the lighting heat load is the most significant parameter. The strawberry light intensity requirement is the high lighting heat load. Consequently, the electricity for the air conditioner becomes high since the air conditioner removes the generated heat from the high light intensity. Therefore, the air conditioner electricity consumption is enormous in this study. Moreover, the required lighting intensity, photoperiod, and low air temperature factors affect electricity consumption. Therefore, the results from this study could provide strategies for energy cost reduction and plantation management for plant factories cultivation.

Storage of Fresh Yam (Dioscorea alata L.) under Controlled Conditions

Freshly harvested "Florido" yam tubers (a highly desirable variety of the species D. alata) were subjected to a temperature of around 32° C. (90° F.) and a relative humidity of 90 percent. Wounded tissues were suberized in about 4 days. This curing may be accomplished either in controlled environmental rooms or under tropical prevailing ambient conditions. The formation of the cork tissue during suberization protects tubers from infection by microorganisms if stored under reduced temperatures. Cured and uncured tubers were stored in a properly ventilated Forma Scientific room at 16° C. ± 1° (59° to 62.9° F.), and a relative humidity of 70 percent. Uncured samples suffered high losses due to physiological breakdown as well as markedly higher weight losses than similarly handled cured ones which showed no signs of internal decay during the storage period. Cured tubers kept under conditions noted above, at temperatures below those prevailing in tropical areas, had the dormant period lengthened by 4 additional months. High-quality tubers can be provided by this means for the fresh market and for processing during the off-season. Although the yam tubers hardened slightly during storage, weight losses were kept at a fairly low level and the palatability of the tubers remained very high, as determined by sensory evaluation.

Ocular surface response of two preservative-free cylcosporine A emulsion eye drops in a mouse model of dry eye

ABSTRACT Purpose/Aim Dry eye (DE) disease is a multifactorial disease in which uncontrolled inflammation can lead to corneal epithelium lesions and symptoms of discomfort. The aim of the present study was to evaluate the efficacy of two cyclosporine emulsions in a mouse model of DE with corneal epithelium lesions. Materials and Methods Six- to 9-week-old female C57BL/6 N mice were housed in a controlled-environment room to induce DE. Following DE development, mice were instilled with: QD 0.1%CsA cationic emulsion (CaEm), BID 0.05%CsA anionic emulsion (AEm), or left untreated. Aqueous tear production and corneal epithelium lesions were assessed throughout the experiment. At the end of the treatment period, left eyes were sampled, fixed, and stained for histology, while the cornea, conjunctiva, and lacrimal gland of right eyes were sampled for transcriptomic analysis. Results Corneal lesion scores were reduced by 10.4%, 18.4%, and 10.9% at day 6, 10, and 14, respectively, with CaEm (QD), and by 2.6%, 3.0%, and 5.5% at day 6, 10, and 14, respectively, with AEm (BID). Histology demonstrated that 7 out of 10 DE mice presented moderate to severe ocular lesions, while only 2 and 5 out of 10 mice presented slight to moderate ocular lesions when treated with the CaEm (QD) and AEm (BID), respectively. The transcriptomic profile analysis suggests that a different set of inflammatory genes are modulated in the cornea, conjunctiva, and lacrimal gland upon DE development. In addition, the two emulsions distinctively modulate the gene expression profile. Conclusions This study demonstrates that both emulsions were effective at reducing corneal lesions, with the CaEm (QD) being slightly better than the AEm (BID). Interestingly, this study suggests that ocular tissues may not respond similarly to a dry environment and that a different set of genes is modulated by the two formulations in the ocular tissues.

CHEMICAL DESICCANTS FOR ANTICIPATION OF HARVEST AND PHYSIOLOGICAL QUALITY OF COWPEA SEEDS

ABSTRACT Information about the effect of herbicides on cowpea seed quality is scarce. The objective of this work was to evaluate the effect of using chemical desiccants at pre-harvest on the anticipation of harvest and physiological quality of cowpea seeds of the cultivar BRS-Tumucumaque, before and after storage. Two experiments were conducted: experiment 1 was conducted under a randomized block design, with four replications; the treatments consisted of seven herbicides (carfentrazone-ethyl, saflufenacil, glyphosate, paraquat, flumioxazin, glufosinate-ammonium, and diquat) and a control treatment; anticipation of harvest, and seed water content, germination, and vigor were evaluated. The seeds were stored for six months in controlled environment and not-controlled environment rooms and evaluated for water content, germination, and vigor in Experiment 2, which was conducted in a 7^2+1 factorial arrangement, with four replications; the first factor consisted of the seven herbicides, the second factor was the two storage environments, plus a control treatment. The results showed 3 to 9 days of anticipation of harvest relative to the control. The glufosinate -ammonium herbicide negatively affected seed germination and vigor. The glyphosate herbicide decreased seed germination and vigor after storage. The physiological quality of cowpea seeds at the post -harvest period and after six months of storage was not affected by the carfentrazone-ethyl and flumioxazin herbicides. Paraquat and diquat were the most efficient herbicides for the anticipation of harvest (nine days). Glyphosate and glufosinate -ammonium were the most harmful herbicides to seed physiological quality. The controlled environment room was the most adequate environment for seed conservation.

A new test setup for simulation of the combined effect of bending and axial restraint in slab-like specimens

This paper presents a long-term experimental campaign for simulation of the combined effects of restrained shrinkage and vertical loads on reinforced concrete (RC) slabs, performed in the scope of the research project “IntegraCrete: a multi-physics and multi-scale approach to the combined effects of applied loads and thermal/shrinkage deformations in reinforced concrete structures”.A new experimental method is applied, in which a restraining device is used to constrain the axial deformation of a slab-like specimen, while allowing it to simultaneously endure bending and shear stresses. The deformation is restrained by two hydraulic actuators connected to one of the slab extremities, allowing a progressive adjustment of the axial force installed whilst controlling the deformation on a pre-defined region (active restraint).The experimental program comprehends the long-term testing of slabs, in a controlled environment room, under four distinct situations by combining the two following conditions: (i) with or without restraint to axial deformations and (ii) with or without application of vertical loads. The structural behaviour of the slabs is continuously monitored over a period of 15 months, while several mechanical properties, as well as the internal relative humidity (RH) of concrete, are assessed in complementary specimens from the same batch.The present work intends to contribute to a deeper understanding of the complex interactions that occur between self-imposed deformations (particularly due to drying shrinkage), concrete viscoelasticity and external loads in the process of crack development. It provides an important and unprecedented database for validation of numerical or analytical approaches for prediction of the structural behaviour of slabs subjected to the abovementioned effects.

Phytophthora cactorum as a Pathogen Associated with Root Rot on Alfalfa (Medicago sativa) in China.

Alfalfa (Medicago sativa) is the largest grown pasture crop in China due to its economic and ecological importance. During the summer season from June to August in 2018, stunted plants was frequently observed in alfalfa fields that have been established for two years in Jinchang, Gansu Province. The disease incidence of root rot ranged from 40% to 50%. Taproots of stunted plants showed red-brown to dark brown discolorations, and lateral roots were poorly developed. Shoots wilted with rotted taproots and lateral roots in severely affected plants. Twenty symptomatic plants were collected and transported to the laboratory for pathogen isolations. Roots were washed under running tap water, cut into 2 to 3 mm pieces (40 pieces each plant), and then sterilized in 75% ethanol for 2 mins followed by three times washing with autoclaved distilled water. Surface dried pieces on autoclaved filter papers were put onto water agar and also a Phytophthora selective medium P5ARP(H) (Jeffers and Martin 1986). The plates were incubated at 22°C for 3 to 5 days and then the growing hypha were subcultured onto potato dextrose agar (PDA). Thirty-two Phytophthora-like isolates were obtained and showed similar morphologies on PDA. Five isolates picked randomly were purified by single-hyphal-tip and plugs (4 to 5 mm) from PDA cultures were incubated in petri dishes with autoclaved distilled water at 22°C for 5 days. Sporangia, chlamydospores and oospores were examined. Sporangia were usually ovoid and sometimes appeared ellipsoid, with the length of 30.5-39.1 μm and width of 23.4-27.8 μm. The diameter of chlamydospores was 29.6 to 42.5μm. Oospores had a diameter of 23.6 to 30.2 μm. The isolates were tentatively identified as P. cactorum based on these morphology characteristics (Montealegre et al. 2016). DNA of these isolates were extracted and PCR amplifications of the rDNA ITS region and cytochrome oxidase subunit I (Cox I) (Kroon et al. 2004) were conducted. Sequences of these isolates were then compared with reference sequences in GenBank using BLAST search. The 866-bp ITS sequences had a sequence identity of 99% to 100% with P. cactorum (e.g. accession nos. EU662221, KJ128036). In addition, the 663-bp CoxI sequences showed 100% sequence identity with three P. cactorum isolates (accession nos. AB688156, HQ708234, EU660851). The ITS and CoxI sequences of one representative isolate Phy.c2 have been deposited in GenBank with the accession no. MT280033 and MT344138, respectively. Pathogenicity of the five isolates (Phy.c1-Phy.c5) were determined on two-week-old alfalfa seedlings (cv. Longdong) grown from seeds. Inoculums were prepared by subculturing agar plugs from edges of PDA cultures into the flask with autoclaved millet seeds, and incubated at 22°C in darkness for two weeks and shaken by hand every two days to ensure uniform colonization. Seedlings were transplanted into pots (12 cm x 12 cm) filled with autoclaved potting mix infested with millet-seed inoculum of each isolate at a rate of 0.5% (w/w). Control seedlings for comparison were transplanted into pots with uninfested potting mix. There were five seedlings per pot and twelve replicate pots for both inoculated and noninoculated treatments, and pots were kept under controlled environment room (22°C, 12 h photoperiod and 65% relative humidity) that were watered every two days to free draining. 87%~92% of the inoculated plants showed stunted symptoms with poorly developed and brown-discoloured roots three weeks after inoculation while the control plants were healthy with no root disease symptoms. To fulfil Koch's postulates, re-isolated cultures from discoloured root tissues were confirmed as the inoculated isolates by morphological examination and ITS sequencing. The five-purified isolates were submitted to the Grassland Culture Collection Center, Lanzhou University, with the accession nos. LZU-MsR-Phy.c1-Phy.c5. To our knowledge, this is the first report of P. cactorum as a pathogen of root rot on alfalfa in China. Phytophthora spp. has been reported causing root rot on alfalfa in America, Australia and Canada, and other legumes such as chickpea, and many other crops worldwide (Musial et al. 2005; Tan and Tan 1986; Vandemark and Barker 2003), and P. cactorum was reported as a root rot pathogen on lavender in China (Chen et al. 2017). P. cactorum may be a significant pathogen associated with root rot in major commercial alfalfa-producing areas in China where are based on flood-irrigation during the growth season.

Biocontamination of surfaces in controlled environment rooms: The influence of environmental parameters and the design of the air conditioning system

This research aims to evaluate the relationship between the environmental and design parameters of the air conditioning system, and the presence of colony-forming units (CFUs) of bacteria on surfaces. This retrospective study was carried out in controlled environment rooms in 12 hospitals in Spain, as part of a periodic environmental biosecurity assessment programme. Between 2010 and 2017, a total amount of 1027 samples were collected from floors and 861 from hand contact surfaces (HCSs), and the environmental parameters (temperature, relative humidity, fungal and bacterial CFUs present in the air) and design parameters (air changes per hour (ACH), differential pressure and position of the high efficiency particulate air filter) were monitored. The multivariate analysis concluded that, for each increased degree Celsius of temperature, the bacterial CFUs increased by 22.4% (incidence rate ratio (IRR): 1.224, 1.05–1.43) and by 25.9% (IRR: 1.259, 1.06–1.50) on HCSs and floors, respectively, and for each increase in ACH, the CFUs decreased 3.9% (IRR: 0.961, 0.94–0.99) and 2.5% (IRR: 0.975, 0.95–1.00) on HCSs and floors, respectively. Our results show the influence of the environmental and design parameters of air conditioning on the number of CFUs on surfaces and floors.