Control Patches Research Articles

Application of high-polarity hydroxyl polyacrylate pressure sensitive adhesive in rizatriptan transdermal drug delivery patch

This study aimed to design a rizatriptan (RIZ) transdermal patch by combining of high-polarity hydroxyl pressure sensitive adhesive (PSA) AAOH-45 with an ion-pair strategy and investigate the molecular mechanism of high content hydroxyl PSA to enhance drug-PSA miscibility. RIZ free base, ion-pair complexes and PSAs containing hydroxyl group were prepared and characterized. Formulation factors including counter-ions, PSAs, drug-loading and others were optimized through single-factor studies and evaluated through pharmacokinetic studies and skin irritation tests. The properties of high polarity PSA and molecular mechanism of drug-PSA miscibility were investigated through molecular simulation, FTIR spectra, 13C NMR spectra, DSC, and rheology study. The optimized formulation contained 20 % (w/w) RIZ-OA (Rizatriptan-Oleic acid), 80 % AAOH-45 (w/w) as the matrix, and had a thickness of 90 μm. Compared with the oral group (MRT0-t = 5.96 ± 0.97 h) and the control patch group (MRT0-t = 11.30 ± 1.78 h), the pharmacokinetic behavior of the optimization group demonstrated sustained drug delivery behavior (MRT0-t = 20.21 ± 0.61 h) with no irritation phenomenon. The miscibility of RIZ with PSAs was positively correlated with the mass percentage of 2-HEA. Higher polar similarity, lower flowability, and stronger intermolecular interaction were responsible for the higher compatibility of high hydroxyl PSA with the drug. This study provided a reference for increasing the drug-loading in PSA and developing RIZ patch.

Flexible Temperature Sensor with High Reproducibility and Wireless Closed-Loop System for Decoupled Multimodal Health Monitoring and Personalized Thermoregulation.

Temperature and pulse waves are two fundamental indicators of body health. Specifically, thermoresistive flexible temperature sensors are one of the most applied sensors. However, they suffer from poor reproducibility of resistivity; and decoupling temperature from pressure/strain is still challenging. Besides, autonomous thermoregulation by wearable sensory systems is in high demand, but conventional commercial apparatuses are cumbersome and not suitable for long-term portable use. Here, a material-design strategy is developed to overcome the problem of poor reproducibility of resistivity by tuning the thermal expansion coefficient to nearly zero, precluding the detriment caused by shape expansion/shrinkage with temperature variation and achieving high reproducibility. The strategy also obtains more reliable sensitivity and higher stability, and the designed thermoresistive fiber has strain-insensitive sensing performance and fast response/recovery time. A smart textile woven by the thermoresistive fiber can decouple temperature and pulse without crosstalk; and a flexible wireless closed-loop system comprising the smart textile, a heating textile, a flexible diminutive control patch, and a smartphone is designed and constructed to monitor health status in real-time and autonomously regulate body temperature. This work offers a new route to circumvent temperature-sensitive effects for flexible sensors and new insights for personalized thermoregulation.

Efficacy and Safety of Topical Tofacitinib for the Treatment of Alopecia Areata.

Alopecia areata (AA) is an autoimmune disease of the hair follicles. Although some cases resolve spontaneously, many patients require some form of treatment, including corticosteroids and vitamin D analogues, among others. Cytokine signaling in autoimmune disorders and their inhibition have been the prime objective in therapeutic research over the past few years. Janus kinase inhibitors such as tofacitinib have shown efficacy in the treatment of AA. The present study aimed to evaluate the efficacy of a novel formulation of topical tofacitinib compared to vehicle in patients with AA. A prospective, non-blinded, intrasubject vehicle-controlled study was conducted in patients with AA for a total duration of 6 months. A 2% tofacitinib citrate ointment was compounded in the pharmacy. Tofacitinib tablets (5 mg) were crushed and mixed in white soft paraffin to produce 2% ointment. A thin layer of this ointment was applied to the treatment patch, while the control patches received the application of the vehicle twice daily. Both patches in each patient were evaluated for percentage change in severity of alopecia tool [SALT] score after 24 weeks as the primary outcome. This was graded as excellent response (>50% improvement), intermediate response (25-50%), mild response (5-25%), and no response (<5% improvement). Trichoscopy and hair pull test were evaluated as secondary outcomes. The present study included 30 patients with AA having a median age of 27 years. Among 30 patients, 40% achieved excellent response (>50% change in the SALT score) over six months of treatment. The mean SALT score was significantly reduced from baseline to six months of treatment (mean [95% CI]: 4.3 [1.9-6.3]; P = 0.001). The control patch had substantially higher positive results in the final hair pull test, indicating disease activity (Treatment: 10% vs. Control: 86.7%, P < 0.001). Compared to the control patch, the prevalence of upright hair (10.0% vs. 80.0%) and terminal hair (3.3% vs. 70.0%) were significantly higher in the treatment patch (P < 0.001). No serious adverse effects were reported during the study duration. Sample size was small and the followup was not long enough to study the full effects of tofacitinib, as well as maintenance of remission or relapse after discontinuation. Topical tofacitinib proved to be an efficacious and well-tolerated treatment modality for AA with no adverse effects reported during this study.

Adolescents’ Intention to Use Long-Acting Reversible Contraception Postpartum

Study ObjectiveMultiparous teens, compared to primiparous teens, are at increased risk for adverse neonatal and maternal outcomes. Long-acting reversible contraception (LARC) is infrequently used among postpartum teens. This study identifies predictors of teens’ intentions to use LARC postpartum when it is widely available. MethodsColorado teens who were patients during their pregnancy in an adolescent-centered clinic where all common methods of contraception were easily accessible were surveyed in clinic during their third trimester and following delivery regarding life circumstances (relationships, stress, and family function) and intended method of postpartum contraception. Multinomial logistic regression analyses were used to examine predictors of intended postpartum contraceptive method: LARC, non-LARC effective (condoms, birth control pills, shot, patch, or ring), or low-effective method or no contraception (abstinence, no method, or undecided). Results1,203 patients were enrolled. Greater life stress was associated with greater likelihood of intending to use low-effective contraception versus LARC postpartum. Teens in a longer relationship with their baby's father (versus those never in a relationship with the baby's father) were less likely to intend to use low-effective contraception or non-LARC effective methods and more likely to intend to use LARC postpartum. ConclusionWhen structural barriers are minimized, non-clinical factors such as relationship context and life stress are most associated with postpartum LARC use intentions. Health care providers can help teen patients obtain the postpartum contraception the patients believe is best by employing developmentally appropriate, person-centered care that is sensitive to life stressors and relationship context.

Mosquitoes do not influence Cotton Mouse (Peromyscus gossypinus) giving-up densities

Abstract It is commonly recognized that predation and the risk of predation can have profound effects on behaviors and population ecology of prey. Comparatively, little attention has been paid to the trade-offs hosts make to manage the risk of parasitism, but there is evidence that hosts make behavioral trade-offs to avoid parasitism in ways that resemble those made by prey animals under the risk of predation. Mosquitoes are common hematophagous ectoparasites which parasitize a wide range of vertebrates, resulting in blood loss, distraction, and disease transmission. Many hosts engage in defensive behaviors to repel or kill mosquitoes and these behaviors likely come at the expense of activities such as foraging and vigilance for predators. We placed a mosquito attractant (BG-Sweetscent) and repellant (allethrin) near artificial foraging patches in areas where mosquitoes were common and measured the amount of grain left after a night of foraging (giving-up density) to investigate whether mosquitoes influenced Cotton Mouse (Peromyscus gossypinus) foraging. We predicted that in patches with mosquito attractants, mosquito harassment would cause mice to engage in defensive behaviors which would distract or deter them from foraging, resulting in less grain consumption relative to control patches. Conversely, we predicted that in mosquito-repellent patches, mice would experience less harassment, resulting in more grain consumption. Indoor arena trials found no evidence that the mosquito treatments influenced cotton mouse behavior in the absence of mosquitoes. However, we found no evidence that mosquito treatments affected giving-up densities.

Comparative Effects of Wild Boar (Sus scrofa) Rooting on the Chemical Properties of Soils in Natural and Post-Fire Environments of the Edough Forest Massif (Northeastern Algeria)

Wild boars use a wide range of habitats. Their invasive nature is gaining attention due to the complexity of its impact. The goal of this research is to analyze the impact of the wild boar on the chemical properties of soils in a natural and a post-fire forest in the Edough Forest Massif in Algeria. This study compares the impact of wild boar rooting on soil parameters to determine the functional role of the wild boar. The research was conducted during the winter of 2022. The study sites included a natural forest and a post-fire area. Rooting tracks were geolocated and soil samples were collected. The results show significant differences between rooted and control patches in the chemical parameters measured in the two environments. However, in the natural environment, significant differences were only noted for the calcium content and electrical conductivity. But in the post-fire environment, strong significant differences were observed for all measured parameters, suggesting that wild boars do not exert a noticeable soil homogenization effect on the soil properties. This research highlights the importance of understanding and managing the impact of wild boars in natural and post-fire forests on soil formation processes, the diversity of soil properties, and their magnitude.

Silver Ion Decreases Foreign Body Reaction and Venous Neointimal Hyperplasia through the Inhibition of Interleukin-33 Expression

Introduction: Vascular prosthetic grafts are widely used in vascular surgery; however, graft infection remains a major concern. Silver-coated vascular grafts have demonstrated anti-infection properties in clinical settings; however, whether the silver irons influence foreign body reaction or neointimal hyperplasia remains unclear. Methods: Sodium alginate and hyaluronic acid (SA/HA) hydrogel patches loaded with rhodamine, with or without silver, were fabricated. Patches were implanted in the subcutaneous or abdominal cavity and inferior vena cava of rats. Samples were harvested on day 14 and examined via immunohistochemical and immunofluorescence analyses. Results: Silver hydrogel was found to decrease the foreign body reaction; after subcutaneous and abdominal cavity implantation in rats, the capsule was found to be thinner in the silver hydrogel group than in the control hydrogel group. The silver hydrogel group had fewer CD68-positive cells and proliferating cell nuclear antigen and interleukin-33 (IL-33) dual-positive cells than the control hydrogel group. Additionally, the silver hydrogel patch reduced the neointimal thickness after patch venoplasty in rats, and the number of IL-33- and IL-1β-positive cells was lower than that in the control patch. Conclusion: Silver-loaded SA/HA hydrogel patches decreased the foreign body reaction and venous neointimal hyperplasia in rats by the inhibition of IL-33 expression.

Pharmacodynamic Studies of Pravastatin Sodium Nanoemulsion Loaded Transdermal Patch for Treatment of Hyperlipidemia

Pravastatin sodium (PVS) is a hypolipidemic drug with poor oral bioavailability due to the first-pass effect. Therefore, this study aims to formulate and evaluate transdermal patches containing PVS-loaded nanoemulsions (PVS-NEs) to increase PVS’s hypolipidemic and hepatoprotective activities. PVS-NEs were prepared using the aqueous titration method, where oleic acid was chosen as an oil phase, and span 80 and tween 80 were used as surfactant and cosurfactant respectively. Droplet size (DS), polydispersity index (PDI), zeta potential (ZP), clarity, and thermodynamic stability of NEs were all characterized. Also, PVS-NEs (NE2) with 50% oil phase, 40% SC mix 2:1, and 10% water were selected as an optimum formula based on the results of DS (251 ± 16), PDI (0.4 ± 0.16), and ZP (-70 ± 10.4) to be incorporated into a transdermal patch, and PVS-NE2 loaded transdermal patches (PVS-NE2-TDPs) were prepared by solvent evaporation method. F1 patch with HPMC E15 and PVP K30 in a ratio of 3:1 represented satisfactory patch properties with good drug-excipients compatibility. Thus, it was selected as an optimum patch formula. The optimized F1 patch was characterized for thickness, moisture content, weight variation, and drug-excipients incompatibility. Therefore, it was subjected to ex vivo skin permeation and finally pharmacodynamic studies. Ex vivo permeation studies of F1 revealed that the cumulative amount of PVS permeated across rat skin was 271.66 ± 19 µg/cm2 in 72 h, and the pharmacodynamic studies demonstrated that the F1 patch was more effective in treating hyperlipidemia than PVS-TDP (control patch) based on both blood analysis and histopathological examination. Graphical

Topical sodium valproate-loaded nanospanlastics versus conventional topical steroid therapy in alopecia areata: a randomized controlled study

BackgroundA myriad of therapeutic modalities for alopecia areata are available; however, none is of high level of evidence, creating an immense need for the evaluation of other treatment modalities, of which topical sodium valproate is of potential role via proposed decrease in beta-catenin breakdown, despite its well-known side effect of hair fall as an oral therapy.ObjectiveEvaluating the efficacy and the safety of sodium valproate (SV)-loaded nanospanlastics, in comparison to topical corticosteroids, this is the currently available gold standard topical treatment for patchy AA.MethodologyA total of 66 patients with patchy AA were randomly assigned to receive either topical mometasone furoate lotion or topical SV applied twice daily to all patches except a control patch, which was left untreated. Clinical, trichoscopic and biochemical assessments of beta-catenin tissue levels and Axin-2 gene expression were carried out at baseline and after 3 months.ResultsBoth therapeutic modalities were comparable. Potential efficacy was highlighted by significant improvement in the representative patch, the largest treated patch, to the control patch, the smallest untreated patch in both steroid and valproate groups (p = 0.027, 0.003 respectively). Both beta-catenin levels and Axin-2 gene expression were reduced after treatment, pointing to the inhibitory effect of dominating uncontrolled inflammatory milieu. Baseline beta-catenin was found to significantly negatively correlate with improvement in the representative patch in patients with baseline level above 0.42 ng/ml (p = − 0.042).ConclusionBoth topical SV and steroids are of comparable modest efficacy. Thus, further evaluation of SV is due in combination with intralesional steroids and other anti-inflammatory treatment modalities, together with developing individualized approaches based on baseline beta-catenin level.ClinicalTrials.gov IdentifierNCT05017454, https://clinicaltrials.gov/ct2/show/NCT05017454.

COULD A DECELLULARIZATION PROTOCOL BE VALIDATED WITHOUT TALKING ABOUT HUMAN LEUKOCYTE ANTIGEN?

Decellularization techniques have advanced to reduce the risk of immune rejection in transplantation. Validation of these protocols typically relies on Crapo's criteria1, which include the absence of visible nuclei and low DNA content. In our study, five decellularization protocols were compared to determine the optimal approach for human fascia lata (HFL) samples. However, our findings raised questions as to why recipients can still develop immunity despite meeting validation criteria.HFL samples were decellularized using four protocols with SDS-Triton X100-DNase (D1 to D4-HFL) and one protocol using solvent-detergent-based baths (D5-HFL). The decellularized samples (D-HFL) were compared to native samples (N-HFL) using histology, and DNA content was measured. The human leukocyte antigen (HLA) content within the matrix was assessed using western blot analysis. Both D-HFL and N-HFL samples, along with negative control patches, were implanted in the backs of 28 Wistar rats. Anti-human IgG serum levels were evaluated after one month.H&amp;E and Hoechst staining revealed the absence of residual cells in all decellularization protocols. DNA content was consistently below the critical threshold (p&lt;0.05). All implanted D-HFL samples resulted in significantly lower anti-human IgG levels compared to N-HFL (p&lt;0.01). However, 2.5 out of 4 rats developed immunity after being implanted with D1 to D4-HFL, with varying levels of anti-human IgG. Only rats implanted with D5-HFL showed undetectable levels of IgG and were considered non-immunized. Western blot analysis indicated that only D5-HFL had a residual HLA content below 1%.The literature on decellularization has primarily relied on Crapo's criteria, which do not consider the role of HLA mismatch in acute immune rejection. Our results suggest that a residual HLA content below 1% should also be considered to prevent immunization, even if other validation criteria are met. Further research is needed to evaluate the impact of residual HLA levels on human allotransplantation outcomes.

In vivo application of a glutaraldehyde-free, UVA/riboflavin cross-linked bovine pericardium confirms suitability for cardiovascular substitutes

Glutaraldehyde (GA)-treated bovine pericardium is still the gold standard for the fabrication of bioprostheses needed for the surgical treatment of valvular malfunction. Although excellent stability and low immunogenicity are accomplished, the application of GA is considered to be causal for structural valve deterioration, diminishing the long-term durability of bioprosthetic tissue. The novel GA-free SULEEI-treatment of bovine pericardium combines decellularization, riboflavin/UVA-cross-linking, and low-energy electron beam irradiation. In the present study, we initiated an in vivo application. We used a subcutaneous rat model to compare the immune and tissue responses, calcification propensity, and biomechanical properties of the alternatively prepared SULEEI bovine pericardial tissue with standard glutaraldehyde-fixed and industrially produced bovine pericardial patch material. SULEEI pericardium evokes a similar immune reaction and tissue response as the control standard bovine patch material. The calcification propensity of SULEEI tissue was low, and biomechanical analysis revealed a heterogeneous but similar pattern in tissue stiffness compared to the control patch. The results of this study highlight the potential of SULEEI-treated bovine pericardial tissue as a candidate for cutting-edge cardiovascular and valvular biomaterials in reconstructive surgery.

Efficiency of emergency conservation methods: a case study of the alcon blue butterfly (Phengaris alcon) and its specific parasitoid

A complicated life history and specific habitat requirements make the Alcon blue butterfly Phengaris alcon a very sensitive species to land-use change. Eggs are oviposited on some Gentiana and Gentianella plants, in whose flowerheads larvae initially feed, after which they continue their development as social parasites in the nests of specific Myrmica ants, once they have been adopted by workers. One of the main threats to their existence is mowing at a time when most individuals are still on gentians. In our research, we tested an emergency conservation method on a site of the butterfly subjected to this kind of pressure in the Białowieża Forest (NE Poland). Previous studies showed that the focal population (dependent on Gentiana pneumonanthe and Myrmica scabrinodis) was characterized by an extremely high density of adults as well as of Ichneumon eumerus, i.e. the rare and specific parasitoid of P. alcon. In mid-August 2020 we performed an unplanned experiment to save caterpillars present in freshly cut plants. We selected about 1800 shoots bearing the eggs or egg shells of the butterfly. Seven hundred and fifty preadoption larvae were transported to the site and placed close to the nests of host ants located in patches without G. pneumonanthe. In the following season we observed that the P. alcon infestation rate of experimental colonies was similar to a control patch, and proportions of parasited pupae did not differ, either.Implications for conservation. Our experiment proved that such actions can be highly effective as emergency conservation methods, and possibly also in (re)introductions of P. alcon.

Towards a functional understanding of rehabilitated urban road verge grasslands: Effects of planting year, site conditions, and landscape factors

Urbanization causes ecosystem degradation and losses of biodiversity. Still, urban landscapes favor organisms, depending on how well they fit the anthropogenic conditions. Creating urban green spaces of high ecological quality, such as pollinator-friendly road verges, promotes biodiversity in cities. We sowed a seed mixture consisting of 26 native plant species with diverse functional traits in 46 road verge patches along four urban roads in Munich (South Germany) in 2019/20 and monitored in 2020/21. Control were pre-existing turfgrass patches with conventional management. We assessed species richness, Shannon diversity, and Pielou's evenness, whereas functional composition was evaluated via functional richness, evenness, and divergence, based on 13 plant traits. To study urbanization effects on species and functional composition, we analyzed soil characteristics and tree shading at the local level, as well as distance to city center, imperviousness, and edge density at the landscape level. We used linear models and ordination analysis to assess treatment, local and landscape effects, and the influence of planting year on taxonomic and functional composition. Rehabilitation treatment explained most differences in species and functional composition. Species richness was lower in the city center and at sites with high soil organic matter; Pielou's evenness increased with soil pH. Trait composition differed between rehabilitated and control patches, and varied according to planting year. Soil bulk density negatively affected functional richness for all traits combined, and edge density reduced functional evenness of establishment traits. We conclude that urban filtering, in combination with planting year, shapes species and trait composition of rehabilitated road verges. Hence, local and landscape-level trait–environment interactions affect the assembly of road verge grasslands. Finally, our findings suggest that establishment traits have a pivotal role in the development of rehabilitated road verges, while the predictability of rehabilitation outcomes may be hindered even when seeding specifically designed seed mixtures.

Patchiness caused by aphid herbivory and ozone events affects aphid's spread

AbstractAphids exploit different patches that offer a favourable habitat during their life cycle. Continuous colonization of new patches is critical for the survival of these insect pests. We developed a mesocosm experimental system to study the effects of stress regimes on the colonization of plants by aphids. In the first experiment, we treated Eruca sativa Mill (arugula) plants with either low ozone (O3) (ambient air with no O3 added), elevated O3 (ambient air with O3 addition) or low O3 + Myzus persicae aphid feeding, with subsequent observation of natural aphid infestation over time. The second experiment consisted of aphid sources and differently treated aphid sinks. The aphid sources were also exposed to either low or high O3 levels. The colonization of the sinks was then monitored over time and distance from source. The infestation rate was increased if the patches had been recently exposed to aphid herbivory. On the contrary, aphids did not colonize patches that experienced a recent O3 episode. The patches of E. sativa plants without prior biotic or abiotic stress were colonized by aphids at the beginning of the experiment, but infestation was poor and most of the plants remained aphid‐free throughout the experiment. Aphids from control patches had a better chance of successfully disseminate on hosts with previous herbivory, and aphid spread from O3‐stressed plants was generally poor. Hence, the colonization risk for a generalist aphid plague will depend on the space–time arrangement between crop patches with different stress levels. Ozone contamination could have a negative effect on the spread infestation of aphids in annual crops, because of habitat fragmentation. Here, we present important information to understand how physiological traits of individuals can be scaled up to predict changes within populations in a scenario of climate change.

Integrated Effects of Thai Essential Oil and Balance Exercise on Parameters associated with Falls in Older Adults at Risk of Falling: A Randomized Controlled Study.

Reducing the risk of falling by improving balance and leg strength may be a preventive strategy. This study evaluated the integrated effects of Thai essential oil and balance exercises on parameters associated with Falls in community-dwelling older adults at risk of falling. Fifty-six participants were randomly allocated to either the intervention group (IG), which performed balance exercises while smelling Thai essential oil scents of Zanthoxylum limonella (Dennst.) Alston, or the control group (CG), which performed balance exercises while receiving a control patch. Balance exercises were practiced for 12, 30-minute sessions over 4 weeks. Static and dynamic balance with eyes open and eyes closed (EC), leg muscle strength, agility, and fear of falling were assessed at baseline, after the 4-week intervention, and at 1 month after the last intervention session. Both groups showed significant improvements in static and dynamic balance, ankle plantarflexor strength, and agility after the 4-week intervention (p&lt;0.05), which persisted at the 1-month follow-up (p&lt;0.05). Compared to the CG, the IG demonstrated significantly better static balance in terms of elliptical sway area (p=0.04) and center of pressure (CoP) velocity (p=0.001) during EC, as well as ankle plantarflexor strength (p=0.01). The IG also maintained a significantly greater improvement in CoP velocity during EC (p=0.01). Integrated Thai essential oil and balance exercises improved static balance and ankle plantarflexor strength compared to the balance exercise with a control patch in older adults at risk of falling.

Psammophytes as traps for beach litter in the Strait of Messina (Mediterranean Sea)

In the present paper, for the first time, the presence of beach litter in dunal plants has been verified in a Strait area in an island. The aims of the present study were to evaluate if the psammophytes can act as a trap for the beached litter, to compare beach litter in plants located in beaches with different anthropogenic impacts, and to describe a new approach (namely clean patch index, CPI) to evaluated the cleanliness of dunal plants. In total 738 macro litter items, belonging to 7 litter categories were found in the study area. The main litter category collected was plastic, followed by ceramic, glass, paper/cardboard, metal, sanitary waste, and medical waste. Our results showed that psammophytes acted as a sink for the beach litter by trapping it. The CPI was higher in the dunal plants than in the relative control patches. The average dissimilarity between “Montorsoli” and “Pylon” beaches was 88.7%. The highest contribution to the dissimilarity between the two beaches was the construction material, followed by polystyrene and plastic pieces. Our data could be important for the implementation of the monitoring program within the Marine Strategy Framework Directive, and may also contribute to the development of specific management of the clean-up measures as well as implement National and Regional action plans.

A low-tech method for monitoring survival and growth of coral transplants at a boutique restoration site.

Coral reef restoration projects are becoming a popular corporate environmental responsibility activity at hotel resorts. Such involvement of private businesses offers the potential to expand restoration into a new socioeconomic sector. However, the scarcity of user-friendly monitoring methods for hotel staff, but robust enough to detect changes over time, hinders the ability to quantify the success or failure of the restoration activity. Here, we present a monitoring method of easy application by hotel staff, without scientific training, using the standard resources available at a hotel resort. Survival and growth of coral transplants were evaluated over 1 year at a boutique coral reef restoration site. The restoration was tailored to the needs of a hotel resort in Seychelles, Indian Ocean. A total of 2,015 nursery-grown corals of branching (four genera, 15 species), massive (16 genera, 23 species), and encrusting (seven genera, seven species) growth types were transplanted to a 1-3 m deep degraded patch reef. A unique cement mix was used to transplant corals onto the hard substrate. On the north side of each coral selected for monitoring, we attached an 8.2 cm × 8.2 cm reflective tile. We used reflective tiles instead of numbered tags due to the expected amount of biofouling growing on the tag surface. Every coral was recorded with top view photography (perpendicular to the plane of coral attachment), with the reflective square in the field of view. We drafted a map of the site to facilitate navigation and re-sighting of the monitored colonies. Then, we developed a simple monitoring protocol for hotel staff. Using the map, and the reflective tiles, the divers located the coral colonies, recorded status (alive, dead, bleaching), and took a photograph. We measured the two-dimensional coral planar area and the change in colony size over time using contour tissue measurements of photographs. The monitoring method was robust enough to detect the expected survival of coral transplants, with encrusting and massive corals outperforming branching corals. Survival of encrusting and massive corals was higher (50%-100%) than branching corals (16.6%-83.3%). The change in colony size was 10.1 cm2 ±8.8 (SE). Branching coral survivors grew faster than massive/encrusting corals. A comprehensive approach to the boutique restoration monitoring experiment would have included comparisons with a control patch reef with a similar species composition to the coral transplants. However, the ability to monitor such a control site, in addition to the restoration site, was beyond the logistic capabilities of the hotel staff, and we were limited to monitoring survival and growth within the restoration site. We conclude that science-based boutique coral reef restoration, tailored to the needs of a hotel resort, combined with a simple monitoring method, can provide a framework for involving hotels as partners in coral reef restoration worldwide.

Succession of the sessile benthic community at a coral reef restoration site.

Introduction: Ecosystem restoration facilitates ecological succession. When a coral reef experiences a disturbance, the community of sessile benthic organisms can follow a successional trajectory that favors the dominance of coral or a change of state to an ecosystem dominated by algae. &#x0D; Objective: To better understand the impact of coral transplants on succession of the sessile benthic community, this study &#x0D; Methods: To measure and monitor the coral cover (cm2) of Pocillopora spp., and the composition of the associated benthic community, experimental and control coral reef patches were established at the coral restoration site in Golfo Dulce, South Pacific Costa Rica. Thirty Pocillopora spp. colonies were attached to nails on the substrate in an experimental patch. The control coral patch contained nails with non-transplanted colonies. Both treatments were photographed monthly during a period of eight months. Changes in the coverage of coral and other sessile benthic organisms were measured from the images and compared over time between the experimental and control patches. &#x0D; Results: The coral transplants experienced bleaching events in August through September 2019 and January through February 2020. The first bleaching event possibly due to sedimentation, and the second to high temperatures. By the end of the experiment, 83 % of the colonies had survived. The live colonies grew significantly following transplantation; &gt; 67 % of their initial coverage area after eight months. In the experimental patch, the areas of Pocillopora spp., coralline crustose algae (CCA), and cyanobacteria increased while the area of algal turf decreased. The increase in coral coverage and CCA, and decrease in algal turf in the experimental patch could be due to herbivores attracted to transplants. The increase in cyanobacteria in the experimental patch could be the result of higher temperatures and may have been a factor in the death of colonies. &#x0D; Conclusions: The transplantation of Pocillopora spp. colonies in Golfo Dulce changed the early successional trajectory of the sessile benthic community to favor the dominance of coral dominance in the experimental patch. These results may be useful in informing expectations for future restoration efforts.

Change in the composition of fauna associated with Pocillopora spp. (Scleractinia, Pocilloporidae) following transplantation

Introduction: Associated fauna comprises most of the diversity of a coral reef and performs ecological functions essential to the reef’s survival. Since Pocillopora corals harbor an important associated fauna, reef restoration efforts are underway in Golfo Dulce, Costa Rica, to preserve them. &#x0D; Objective: To describe changes in cryptofauna and fish communities associated with Pocillopora colonies to better understand the succession of associated fauna following transplantation. &#x0D; Methods: An experimental patch of 30 nursery-grown Pocillopora colonies and a control patch containing no colonies were monitored for 8 months following transplantation in Golfo Dulce. Cryptofauna within each colony and fish within each patch were observed using SCUBA to quantify temporal changes in the abundance, diversity, and community structure of the colonies. &#x0D; Results: The abundance and diversity of cryptofauna increased throughout the experiment. Obligate symbiont decapods were the most abundant. The composition of the community of cryptofauna differed between periods with fish in the genus Scarus as the main contributor to any differences. The increase in abundance and diversity of cryptofauna and fish may reflect coral growth and the corresponding availability of space and environmental complexity in the experimental patch. The composition of the cryptofauna communities was generally consistent with other studies. However, a high density of decapod symbionts could suggest that without other Pocillopora colonies to move to, they may crowd together despite their aggressive tendencies. &#x0D; Conclusions: Pocillopora colonies will experience an increase in symbionts that could positively contribute to the health and survival of the coral following transplantation.

Community-managed coral reef restoration in southern Kenya initiates reef recovery using various artificial reef designs

Monitoring of reef restoration efforts and artificial reefs (ARs) has typically been limited to coral fragment survival, hampering evaluation of broader objectives such as ecosystem recovery. This study aimed to determine to what extent AR design influences the ecological recovery of restored reefs by monitoring outplanted coral fragments, benthic cover, coral recruitment and fish and invertebrate communities for two years. Four AR designs (16 m2), unrestored controls and natural reef patches as reference (n = 10) were established in Mkwiro, Kenya. ARs consisted either of concrete disks with bottles, layered concrete disks, metal cages or a combination thereof. A mixture of 18 branching coral species (mainly Acropora spp.) was outplanted on ARs at a density of 7 corals m-2. After two years, 60% of all outplanted fragments had survived, already resulting in coral cover on most ARs comparable (though Acropora-dominated) to reference patches. Coral survival differed between ARs, with highest survival on cages due to the absence of crown-of-thorns sea star predation on this design. In total, 32 coral genera recruited on ARs and recruit densities were highest on reference patches, moderate on concrete ARs and low on cages. ARs and reference patches featured nearly twice the fish species richness and around an order of magnitude higher fish abundance and biomass compared to control patches. Fish abundance and biomass strongly correlated with coral cover on ARs. AR, reference and control patches all had distinct fish species compositions, but AR and reference patches were similar in terms of trophic structure of their fish communities. Motile invertebrates including gastropods, sea urchins, sea cucumbers and sea stars were present at ARs, but generally more abundant and diverse at natural reference patches. Taken together, all studied ecological parameters progressed towards reef ecosystem recovery, with varying influences of AR design and material. We recommend a combination of metal cages and layered concrete ARs to promote high fragment survival as well as natural coral recruitment. Ultimately, a longer period of monitoring is needed to fully determine the effectiveness reef restoration as conservation tool to support coral reef ecosystem recovery.