Control Indomethacin Research Articles

The effect of indomethacin on the cytokine cascade and body temperature following burn injury in rats

This study investigates the hypothesis that indomethacin's ability to prevent “fever” following burn injury in rats is mediated via decreased plasma concentrations of IL-6, the putative mediator of increased body temperature. Sprague–Dawley rats had radio transmitters and osmotic pumps containing indomethacin placed in the peritoneal cavity. Seven days later full thickness scald burns to 50% of the body surface area were produced. Following burn injuries, daily blood samples were obtained from a carotid catheter for assay of lipopolysaccharide (LPS), interleukin-1α (IL-1α), IL-1β, tumor necrosis factor-α (TNF-α) and IL-6. In addition, body temperature ( T B) and activity index were obtained every five minutes by telemetry. There were four experimental groups: burn+indomethacin (B-In); burn+polyethylene glycol (Peg) (B-Peg); control+indomethacin (C-In); and control+Peg (C-Peg). Burned animals demonstrated a significant two-fold increase in plasma IL-1α levels ( p=0.004) and a seven-fold increment in IL-6 ( p=0.0001) through the 7th PBD, and indomethacin administration had no significant effect upon the cytokine plasma levels. There were no significant increases in IL-1β, TNF-α or LPS in any group. Indomethacin eliminated the chronic increase in T B following burn injury, and this effect was not produced by changes in plasma levels of the endogenous pyrogens IL-1α and IL-6.

Chronic Indomethacin Administration Blocks Increased Body Temperature After Burn Injury in Rats

This study investigates the hypothesis that continuous administration of indomethacin (a cyclo-oxygenase inhibitor) will chronically reduce body temperature (TB) in burned rats (i.e., modulate "true" fever). Male Sprague-Dawley rats had radio transmitters and osmotic pumps (containing indomethacin) placed in the peritoneal cavity, and 7 days later large full-thickness scald burns were produced. Activity and TB were continually recorded through the 14th postburn day (PBD). There were 4 experimental groups: burn + indomethacin (B-In), n = 9; burn + polyethylene glycol (B-Peg), n = 6; control + indomethacin (C-In), n = 9; and control + polyethylene glycol (C-Peg), n = 6. From PBD 5 through PBD 10, the B-Peg group had consistently and significantly higher TB during light hours than the B-In, C-In, and C-Peg groups. From PBD 7 through PBD 12, the B-In group had an average TB during light hours significantly lower or not different than the C-In and C-Peg groups. These results support the hypothesis that in this burned-rat model chronically increased TB during the light hours may be "fever" (rather than hyperthermia) and that the final effector link could be a prostanoid because changes in activity do not explain the changes in body temperature.