Abstract. As drought and prolonged water stress become more prevalent in dry regions under climate change, preserving water resources becomes a focal point for maintaining forest health. Forest regeneration after forest loss or disturbance can lead to overstocked juvenile stands with high water demands and low water-use efficiency. Forest thinning is a common practice with the goal of improving tree health, carbon storage, and water use while decreasing stand demands in arid and semi-arid regions. However, little is known about the impacts of stand density on seasonal variation in depth-to-water uptake or the magnitude of the effect of growing season drought conditions on water availability. Existing reports are highly variable by climatic region, species, and thinning intensity. In this study, stable isotope ratios of deuterium (δ2H) and oxygen (δ18O) in water collected from various soil depths and from branches of lodgepole pine (Pinus contorta) under different degrees of thinning (control: 27 000 stems per hectare; moderately thinned: 4500 stems per hectare; heavily thinned: 1100 stems per hectare) over the growing season were analyzed using the MixSIAR Bayesian mixing model to calculate the relative contributions of different water sources in the Okanagan Valley in the interior of British Columbia, Canada. We found that under drought conditions the lodgepole pine trees shifted their depth-to-water uptake through the growing season (June to October) to rely more heavily on older precipitation events that percolated through the soil profile when shallow soil water became less accessible. Decreased forest density subsequent to forest thinning did not cause a significant difference in the isotopic composition of branch water but did cause changes in the timing and relative proportion of water utilized from different depths. Thinned lodgepole pine stands were able to maintain water uptake from 35 cm below the soil profile, whereas the overstocked stands relied on a larger proportion of deep soil water and groundwater towards the end of the growing season. Our results support other findings by indicating that, although lodgepole pines are drought-tolerant and have dimorphic root systems, they do not shift back from deep water sources to shallow soil water when soil water availability increases following precipitation events at the end of the growing season.