Contribution Of Different Domains Research Articles

Overcoming the Barrier of Incompleteness: A Hyperspectral Image Classification Full Model.

Deep learning-based methods have shown promising outcomes in many fields. However, the performance gain is always limited to a large extent in classifying hyperspectral image (HSI). We discover that the reason behind this phenomenon lies in the incomplete classification of HSI, i.e., existing works only focus on a certain stage that contributes to the classification, while ignoring other equally or even more significant phases. To address the above issue, we creatively put forward three elements needed for complete classification: the extensive exploration of available features, adequate reuse of representative features, and differential fusion of multidomain features. To the best of our knowledge, these three elements are being established for the first time, providing a fresh perspective on designing HSI-tailored models. On this basis, an HSI classification full model (HSIC-FM) is proposed to overcome the barrier of incompleteness. Specifically, a recurrent transformer corresponding to Element 1 is presented to comprehensively extract short-term details and long-term semantics for local-to-global geographical representation. Afterward, a feature reuse strategy matching Element 2 is designed to sufficiently recycle valuable information aimed at refined classification using few annotations. Eventually, a discriminant optimization is formulized in accordance with Element 3 to distinctly integrate multidomain features for the purpose of constraining the contribution of different domains. Numerous experiments on four datasets at small-, medium-, and large-scale demonstrate that the proposed method outperforms the state-of-the-art (SOTA) methods, such as convolutional neural network (CNN)-, fully convolutional network (FCN)-, recurrent neural network (RNN)-, graph convolutional network (GCN)-, and transformer-based models (e.g., accuracy improvement of more than 9% with only five training samples per class). The code will be available soon at https://github.com/jqyang22/ HSIC-FM.

A jack of all trades - ADAM8 as a signaling hub in inflammation and cancer.

As a member of the family of A Disintegrin And Metalloproteinases (ADAM) ADAM8 is preferentially expressed in lymphatic organs, immune cells, and tumor cells. The substrate spectrum for ADAM8 proteolytic activity is not exclusive but is related to effectors of inflammation and signaling in the tumor microenvironment. In addition, complexes of ADAM8 with extracellular binding partners such as integrin β-1 cause an extensive intracellular signaling in tumor cells, thereby activating kinase pathways with STAT3, ERK1/2, and Akt signaling, which causes increased cell survival and enhanced motility. The cytoplasmic domain of ADAM8 harbors five SRC homology-3 (SH3) domains that can potentially interact with several proteins involved in actin dynamics and cell motility, including Myosin 1F (MYO1F), which is essential for neutrophil motility. The concept of ADAM8 thus involves immune cell recruitment, in most cases leading to an enhancement of inflammatory (asthma, COPD) and tumor (including pancreatic and breast cancers) pathologies. In this review, we report on available studies that qualify ADAM8 as a therapeutic target in different pathologies. As a signaling hub, ADAM8 controls extracellular, intracellular, and intercellular communication, the latter one mainly mediated by the release of extracellular vesicles with ADAM8 as cargo. Here, we will dissect the contribution of different domains to these distinct ways of communication in several pathologies. We conclude that therapeutic targeting attempts for ADAM8 should consider blocking more than a single domain and that this requires a thorough evaluation of potent molecules targeting ADAM8 in an in vivo setting.

Structural and biochemical characterization establishes a detailed understanding of KEAP1-CUL3 complex assembly.

KEAP1 promotes the ubiquitin-dependent degradation of NRF2 by assembling into a CUL3-dependent ubiquitin ligase complex. Oxidative and electrophilic stress inhibit KEAP1 allowing NRF2 to accumulate for the transactivation of stress response genes. To date there are no structures of the KEAP1-CUL3 interaction nor binding data to show the contributions of different domains to their binding affinity. We determined a crystal structure of the BTB and 3-box domains of human KEAP1 in complex with the CUL3 N-terminal domain that showed a heterotetrameric assembly with 2:2 stoichiometry. To support the structural data, we developed a versatile TR-FRET-based assay system to profile the binding of BTB-domain-containing proteins to CUL3 and determine the contribution of distinct protein features, revealing the importance of the CUL3 N-terminal extension for high affinity binding. We further provide direct evidence that the investigational drug CDDO does not disrupt the KEAP1-CUL3 interaction, even at high concentrations, but reduces the affinity of KEAP1-CUL3 binding. The TR-FRET-based assay system offers a generalizable platform for profiling this protein class and may form a suitable screening platform for ligands that disrupt these interactions by targeting the BTB or 3-box domains to block E3 ligase function.

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay.

Transition metals such as Zn2+ ions must be tightly regulated due to their cellular toxicity. Previously, the activity of Zn2+ transporters was measured indirectly by determining the expression level of the transporter under different concentrations of Zn2+. This was done by utilizing immunohistochemistry, measuring mRNA in the tissue, or determining the cellular Zn2+ levels. With the development of intracellular Zn2+ sensors, the activities of zinc transporters are currently primarily determined by correlating changes in intracellular Zn2+, detected using fluorescent probes, with the expression of the Zn2+ transporters. However, even today, only a few labs monitor dynamic changes in intracellular Zn2+ and use it to measure the activity of zinc transporters directly. Part of the problem is that out of the 10 zinc transporters of the ZnT family, except for ZnT10 (transports manganese), only zinc transporter 1 (ZnT1) is localized at the plasma membrane. Therefore, linking the transport activity to changes in the intracellular Zn2+ concentration is hard. This article describes a direct way to determine the zinc transport kinetics using an assay based on a zinc-specific fluorescent dye, FluoZin-3. This dye is loaded into mammalian cells in its ester form and then trapped in the cytosol due to cellular di-esterase activity. The cells are loaded with Zn2+ by utilizing the Zn2+ ionophore pyrithione. The ZnT1 activity is assessed from the linear part of the reduction in fluorescence following the cell washout. The fluorescence measured at an excitation of 470 nm and emission of 520 nm is proportional to the free intracellular Zn2+. Selecting the cells expressing ZnT1 tagged with the mCherry fluorophore allows for monitoring only the cells expressing the transporter. This assay is used to investigate the contribution of different domains of ZnT1 protein to the transport mechanism of human ZnT1, a eukaryotic transmembrane protein that extrudes excess zinc from the cell.

Control of Magnetohydrodynamic Mixed Convection and Entropy Generation in a Porous Cavity by Using Double Rotating Cylinders and Curved Partition.

In this work, mixed convection and entropy generation analyses in a partitioned porous cavity with double inner rotating cylinders are explored under magnetic field effects. A curved partition shape is considered with identical rotating cylinders and an inclined magnetic field, while the right vertical wall moves with a constant speed in the y-direction. Numerical simulations are performed by considering various values of Rayleigh number, Hartman number, Darcy number, inclination of the magnetic field, size of the curved partitions, and rotational speeds of the inner cylinders and their vertical locations with the cavity. Complicated flow field with multicellular structures are observed due to the complex interaction between the natural convection, moving wall, and rotational effects of inner cylinders. Improved heat-transfer performance is obtained with higher values of magnetic field inclination, higher values of permeability/porosity of the medium, and higher rotational speeds of the cylinders. Almost doubling of the average Nu number is obtained by decreasing the value of the Hartmann number from 25 to 0 or varying the magnetic field inclination from 90 to 0. When rotational effects of the cylinders are considered, average heat-transfer improvements by a factor of 5 and 5.9 are obtained for nondimensional rotational speeds of 5 and −5 in comparison with the case of motionless cylinders. An optimum length of the porous layer is achieved for which the best heat-transfer performance is achieved. As the curvature size of the partition is increased, better heat transfer of the hot wall is obtained and up to 138% enhancement is achieved. Significant increments of entropy generation are observed for left and right domains including the rotating cylinders. The magnetic field parameter also affects the entropy generation and contributions of different domains including the curved porous partition.

Systematic workflow for studying domain contributions of bispecific antibodies to selectivity in multimodal chromatography.

In this article, a systematic workflow was formulated and implemented to understand selectivity differences and preferred binding patches for bispecific monoclonal antibodies (mAbs) and their parental mAbs on three multimodal cation exchange resin systems. This workflow incorporates chromatographic screening of the parent mAbs and their fragments at various pH followed by surface property mapping and protein footprinting using covalent labeling followed by liquid chromatography-mass spectrometry analysis. The chromatography screens on multimodal resins with the intact mAbs indicated enhanced selectivity as compared to single-mode interaction systems. While the bispecific antibody (bsAb) eluted between the two parental mAbs on most of the resins, the retention of the bispecific transitioned from co-eluting with one parental mAb to the other parental mAb on Capto MMC. To investigate the contribution of different domains, mAb fragments were evaluated and the results indicated that the interactions were likely dominated by the Fab domain at higher pH. Protein surface property maps were then employed to hypothesize the potential preferred binding patches in the solvent-exposed regions of the parental Fabs. Finally, protein footprinting was carried out with the parental mAbs and the bsAb in the bound and unbound states at pH 7.5 to identify the preferred binding patches. Results with the intact mAb analysis supported the hypothesis that interactions with the resins were primarily driven by the residues in the Fab fragments and not the Fc. Furthermore, peptide mapping data indicated that the light chain may be playing a more important role in the higher binding of Parent A as compared with Parent B in these resin systems. Finally, results with the bsAb indicated that both halves of the molecule contributed to binding with the resins, albeit with subtle differences as compared to the parental mAbs. The workflow presented in this paper lays the foundation to systematically study the chromatographic selectivity of large multidomain molecules which can provide insights into improved biomanufacturability and expedited downstream bioprocess development.

Distinct Contributions of Different Domains within the HIV-1 Gag Polyprotein to Specific and Nonspecific Interactions with RNA.

Viral genomic RNA is packaged into virions with high specificity and selectivity. However, in vitro the Gag specificity towards viral RNA is obscured when measured in buffers containing physiological salt. Interestingly, when the binding is challenged by increased salt concentration, the addition of competing RNAs, or introducing mutations to Gag protein, the specificity towards viral RNA becomes detectable. The objective of this work was to examine the contributions of the individual HIV-1 Gag polyprotein domains to nonspecific and specific RNA binding and stability of the initial protein-RNA complexes. Using a panel of Gag proteins with mutations disabling different Gag-Gag or Gag-RNA interfaces, we investigated the distinct contributions of individual domains which distinguish the binding to viral and nonviral RNA by measuring the binding of the proteins to RNAs. We measured the binding affinity in near-physiological salt concentration, and then challenged the binding by increasing the ionic strength to suppress the electrostatic interactions and reveal the contribution of specific Gag–RNA and Gag–Gag interactions. Surprisingly, we observed that Gag dimerization and the highly basic region in the matrix domain contribute significantly to the specificity of viral RNA binding.

Cumulative Risk of Substance Use in Community College Students.

Substance use in community college students has been explored in only a handful of studies. Differences in population characteristics and substance use between 2- and 4-year students suggest that different factors may promote and thwart this behavior. Cumulative risk is a parsimonious methodology that provides better model stability and more statistical power, yet it has only been recently used in substance use research. The aim of this study is to investigate multiple aspects of substance use risk in a population in need of substance use prevention and intervention services. We conducted a cross-sectional study of community college students (N = 288; 75% female) examining the relative contributions of different domains of cumulative risk (eg, life stressors, academic stressors, and mental health diagnoses) to develop different profiles of risk across substance use classes (ie, alcohol, cigarette, marijuana, and hard drug use). Cumulative risk analyses indicated that alcohol and tobacco use were associated with the domains of life stressors and peer/family substance use, marijuana use with peer/family substance use and stressful childhood experiences, and hard drug use with peer/family substance use, lack of social support, low access to care, and stressful childhood experiences. Different strategies for prevention and intervention may be necessary to effectively address different forms of substance use in this population. The risk domain profiles related to specific drugs may lead to targeted interventions to reduce substance use in community college students. (Am J Addict 2020;29:97-104).

What is the contribution of each physical activity domain to total physical activity in adolescents?

Abstract The present study aimed to verify the contribution of different physical activity domains to “total physical activity” in Brazilian adolescents. This is a cross-sectional study using secondary data from the third edition of the National School Health Survey - PeNSE, 2015. The sample consisted of 100,497 adolescents of both sexes enrolled in the 9th grade of elementary schools. A linear regression model was used to verify how much each domain contributed to total physical activity, considering gender, type of municipality and region. The domain with the largest contribution to “total physical activity” regardless of sociodemographic and environmental variables was “extra-school physical activity” (R2 = 0.60), followed by “active commuting” (R2 = 0.34), and finally “Physical Education classes” (R2 = 0.23). The contribution of the different domains varied by gender, type of municipality and region, and it was concluded that “extra-school physical activity” make the greatest contribution to “total physical activity”, followed by “active commuting” and “Physical Education classes”. In addition, variation was observed in the contribution of domains by gender, type of municipality and region.

Influence of Length and Amino Acid Composition on Dimer Formation of Immunoglobulin based Chimera.

The dimeric immunoglobulin (Ig) chimeras used for drug targeting and delivery are preferred biologics over their monomeric forms. Designing these Ig chimeras involves critical selection of a suitable Ig base that ensures dimer formation. In the present study, we systematically analyzed several factors that influence the formation of dimeric chimera. We designed and predicted 608 cytokine-Ig chimeras where we tested the contributions of (1) different domains of Ig constant heavy chain, (2) length of partner proteins, (3) amino acid (AA) composition and (4) position of cysteine in the formation of homodimer. The sequences of various Ig and cytokines were procured from Uniprot database, fused and submitted to COTH (CO-THreader) server for the prediction of dimer formation. Contributions of different domains of Ig constant heavy chain, length of chimeric proteins, AA composition and position of cysteine to the homodimer formation of 608 cytokine-Ig chimeras were tested. Various in silico approaches were adopted for validating the in silico findings. Experimentally we also validated our approach by expressing the chimeric design of shorter cytokine with Ig domain in CHO cells and analyzing the protein by SDS-PAGE. Our results advocate that while the CH1 region and the Hinge region of Ig heavy chain are critical, the length of partner proteins also crucially influences homodimer formation of the Ig-based chimera. We also report that the CH1 domain of Ig is not required for dimer formation of Ig based chimera in the presence of larger partner proteins. For shorter partner proteins fused to CH2-CH3, careful selection of partner sequence is critical, particularly the hydrophobic AA composition, cysteine content & their positions, disulphide bond formation property, and the linker sequences. We validated our in silico observation by various bioinformatics tools and checked the ability of chimeras to bind with the receptors of native protein by docking studies. As a proof of concept, we have expressed the chimeric proteins in CHO cells and found that our design favors the synthesis of dimeric proteins. Our structural prediction study suggests that extra amino acids in the range of 15-20 added to the CH2 domain of Ig is a critical requirement to make homodimer. This information from our study will have implication in designing efficacious homodimeric chimera.

Direct Observation of Intermediate State(s) in the Mechanistic Investigation of Domain Specific Protein-Surfactant Interaction

Interaction of surfactants with proteins can decipher important information regarding the stability and behavior of proteins. For multi-domain proteins, these interactions vary domain wise and these details are crucial in understanding the contribution of different domains of the protein in its overall activity. The objective of the present work is to study the interaction of surfactants with domain III of Human Serum Albumin (HSA) and to compare the same with the global interaction. Interaction of the anionic Sodium Dodecyl Sulphate (SDS) and the Cationic Cetyltrimethylammonium Bromide (CTAB) surfactants with domain III of Human Serum Albumin (HSA) has been studied using 8-Anilino-1-Naphthalene-Sulphonate (ANS) as a fluorescent marker. Circular Dichroism (CD) spectroscopy has been used to study the protein-surfactant interaction for the overall protein. SDS is found to interact sequentially with domain III of HSA having two detectable intermediate states in the binding process. In case of CTAB, we have observed only one intermediate state for its interaction with domain III. Although Quantum yield measurement can reflect the presence of such intermediate state, the overall conformational change of the HSA on addition of surfactants, studied by Circular Dichroism (CD) spectroscopy, and the ANS-Trp distance measurement by FRET could not resolve the presence of such intermediate states. The esterase activity of HSA in presence of different amount of surfactants is also in accordance with our above observation. The interaction of both the surfactants with HSA is found to be sequential in nature. The most important conclusion revealed from our study is that the nature of protein-surfactant interaction is not same throughout the entire protein. Our study reveals that different parts of the multi-domain HSA have different affinity to the surfactant molecules.

Domain Swap Approach Reveals the Critical Roles of Different Domains of SYMRK in Root Nodule Symbiosis in Lotus japonicus.

Symbiosis receptor kinase (SYMRK) is a cell membrane-localized protein kinase containing extracellular malectin-like domain (MLD) and leucine-rich repeat (LRR) domains, which is critically required for both root nodule symbiosis (RNS) and arbuscular mycorrhizal symbiosis (AMS). SYMRK is widely distributed in the genomes of different plant species; however, the contribution of different domains of SYMRK and its homologs from other plant species to RNS is largely unclear. In this study, SYMRK and its homologs from three typical plant species including Medicago truncatula (for both RNS and AMS), Oryza sativa (for AMS but not RNS), and Arabidopsis thaliana (for neither RNS or AMS) were investigated using domain swap approach in response to rhizobia in Lotus japonicus. Full-length SYMRK from rice and Medicago but not from Arabidopsis could complement Lotus symrk-409 mutant plants to contribute RNS. The chimeric protein with the extracellular domain (ED) of LjSYMRK and cytoplasmic domains (CD) of SYMRK from both Medicago and rice but not Arabidopsis could contribute to RNS in Lotus, suggesting that the CD of SYMRK is required for symbiotic signaling. The chimeric receptors containing the CD of LjSYMRK (SYMRKCD) and the EDs of MtDMI2 (MtDMI2ED), OsSYMRK (OsSYMRKED), AtSYMRK (AtSYMRKED), NFR1 (NFR1ED), and NFR5 (NFR5ED) could complement Lotus symrk-409 mutant plants to develop nodules. However, MtDMI2 could partially complement Lotus symrk-409 mutants to form both effective nodules and ineffective bumps, which is similar to the complementation results from MtDMI2ED-LjSYMRKCD and LjSYMRKGDLC in Lotus symrk-409 mutants, suggesting that ED of SYMRK has a very fine-tune regulation for RNS in Lotus. The deletion of either MLD or LRR on SYMRKGDLC (a mutant version of SYMRK with GDPC motif replaced by GDLC) could contribute to RNS when overexpressed in Lotus symrk-409 mutants, suggesting that MLD and LRR domains might work together to be involved in symbiotic signaling and the LRR domain might play a negative role in LjSYMRKGDLC-mediated RNS. By mutagenizing the conserved amino acids on LRR domain, five serine residues were found to be required for the function of LjSYMRKGDLC in RNS. These finding precisely refine the molecular mechanisms of SYMRK function in symbiotic signaling in L. japonicus.

Functional and mutational landscapes of BRCA1 for homology-directed repair and therapy resistance.

BRCA1 plays a critical role in homology-directed repair (HDR) of DNA double strand breaks, and the repair defect of BRCA1-mutant cancer cells is being targeted with platinum drugs and poly (ADP-ribose) polymerase (PARP) inhibitors. We have employed relatively simple and sensitive assays to determine the function of BRCA1 variants or mutants in two HDR mechanisms, homologous recombination (HR) and single strand annealing (SSA), and in conferring resistance to cisplatin and olaparib in human cancer cells. Our results define the functionality of the top 22 patient-derived BRCA1 missense variants and the contribution of different domains of BRCA1 and its E3 ubiquitin ligase activity to HDR and drug resistance. Importantly, our results also demonstrate that the BRCA1-PALB2 interaction dictates the choice between HR and SSA. These studies establish functional and mutational landscapes of BRCA1 for HDR and therapy resistance, while revealing novel insights into BRCA1 regulatory mechanisms and HDR pathway choice.

Dynamics of the E. coli beta clamp and its influence on DNA loading

Sliding clamps are found in all domains of life and are essential for efficient DNA replication, which is required every time a cell divides, as well as for coordinating traffic on DNA. The Escherichia coli (E. coli) beta‐clamp is a ring‐shaped protein consisting of two identical monomers encoded by the dnaN gene that associates with DNA polymerase III and facilitates the high degree of processivity required for DNA replication. Sliding clamp proteins open and are loaded onto specific DNA structures by clamp loaders in the presence of ATP. We aim to gain insights into the contributions of different domains to the function of beta using a linked‐beta‐clamp that constrains the homodimer at one of two interfaces. Usually the homo‐dimeric structure of the beta‐clamp allows it to act as a molecular tool belt, binding more than one protein simultaneously at specific protein interaction sites on the clamp. Thus, a better understanding of the sliding clamps' role in the process of DNA damage tolerance will be gathered by investigating whether one interface or binding site is adequate for DNA loading or for other protein interactions. To create this construct, the linker length and sequence, as well as expression conditions, were optimized. The linked‐beta proteins were characterized through a thermal denaturation assay, and showed comparable thermal stability to wild‐type beta. In an ATPase assay, similar amounts of inorganic phosphate were detected in reactions containing either linked‐beta or wild‐type beta, indicating that the clamp loader can interact with both versions of the beta clamp. Currently, we are investigating whether linked and wild‐type beta improve the processivity of a polymerase in a primer extension assay. We are now constructing and characterizing variants of wild‐type beta and linked‐beta to test the importance of residues at only one of the interfaces or partner protein binding sites.

Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions.

The semaphorins are an essential family of axon guidance molecules that can be either secreted or are transmembrane proteins. Class 3 semaphorin (Sema3) family members are secreted and provide long-range guidance cues through two receptor families: neuropilins (Nrp) and plexins. Nrp is uniquely required for high-affinity Sema3 binding and signaling. Therefore, characterizing the molecular details of the Sema3/Nrp interaction is important for understanding the broader physiological and pathological role of the Sema3 family of proteins. Here we describe an in vitro plate-based binding assay for characterization of the Sema3/Nrp interaction. This assay utilizes Nrp-affinity plates and an alkaline phosphatase (AP)-Sema3 fusion to rapidly measure direct Sema3/Nrp binding. This assay can be used to measure receptor-ligand binding, the contribution of different domains, and exogenous factors, and to characterize competitive ligand binding.

Pathways through which asthma risk factors contribute to asthma severity in inner-city children

Pathway analyses can be used to determine how host and environmental factors contribute to asthma severity. To investigate pathways explaining asthma severity in inner-city children. On the basis of medical evidence in the published literature, we developed a conceptual model to describe how 8 risk-factor domains (allergen sensitization, allergic inflammation, pulmonary physiology, stress, obesity, vitamin D, environmental tobacco smoke [ETS] exposure, and rhinitis severity) are linked to asthma severity. To estimate the relative magnitude and significance of hypothesized relationships among these domains and asthma severity, we applied a causal network analysis to test our model in an Inner-City Asthma Consortium study. Participants comprised 6- to 17-year-old children (n=561) with asthma and rhinitis from 9 US inner cities who were evaluated every 2months for 1year. Asthma severity was measured by a longitudinal composite assessment of day and night symptoms, exacerbations, and controller usage. Our conceptual model explained 53.4% of the variance in asthma severity. An allergy pathway (linking allergen sensitization, allergic inflammation, pulmonary physiology, and rhinitis severity domains to asthma severity) and the ETS exposure pathway (linking ETS exposure and pulmonary physiology domains to asthma severity) exerted significant effects on asthma severity. Among the domains, pulmonary physiology and rhinitis severity had the largest significant standardized total effects on asthma severity (-0.51 and 0.48, respectively), followed by ETS exposure (0.30) and allergic inflammation (0.22). Although vitamin D had modest but significant indirect effects on asthma severity, its total effect was insignificant (0.01). The standardized effect sizes generated by a causal network analysis quantify the relative contributions of different domains and can be used to prioritize interventions to address asthma severity.

The subject structure of global science and optimization of the Russian network of dissertation councils: Imbalance and harmonization

This paper reviews the results of the optimization of the network of dissertation councils in the Russian Federation in the period between 2013 and 2015. The disciplinary structure of highly qualified scientific personnel in Russia, which is expected to evolve following the optimization, and the subject structure of global science, are compared. For this purpose, the share of publications in the specific subject areas of the Web of Science database is evaluated. The contribution of different domains to the development of scientific fronts based on the Essential Science Indicators data is assessed. The project was aimed at optimizing dissertation councils on the harmonized structure of highly qualified scientific personnel in Russia; the subject structure of global science was determined relying on factual and image data. It is highlighted that the unjustifiably low share of medical dissertation councils and the high share of dissertation councils for economic sciences, which is not in line with global research trends, will remain in Russia by late 2015.

Patterns of physical activity and sedentary behavior in a representative sample of a multi-ethnic South-East Asian population: a cross-sectional study.

BackgroundFew studies have investigated patterns of physical activity in a multi-ethnic Asian urban population. Even less is known about sedentary behaviors in these populations. The present study examined the prevalence of physical activity, exercise and sedentary behavior. In addition, it investigated socio-demographic correlates and the contribution of different domains towards overall physical activity.MethodsData of 2319 participants from the population-based cross-sectional Singapore Health 2012 study were analyzed. Physical activity, exercise and sedentary behavior were assessed using the Global Physical Activity Questionnaires. A modified Cox regression model was used to estimate the relative prevalence rates (PR) for overall physical activity, leisure-time exercise and high level of sedentary behavior by socio-demographic factors.ResultsOverall, 73.8% of participants met physical activity guidelines, 24.3% did regular leisure-time exercise and 37.0% reported high levels of sedentary behavior. Travel-related activities contributed about half of the total physical activity. There was a consistent association between age of participants with physical activity and exercise. Older participants were less likely to meet the guidelines (PR = 0.74, 95% C I = 0.61 – 0.91) than younger participants. The prevalence of regular exercise was lowest among 30 to 39 years aged participants (PR = 0.62, 95% CI = 0.45 – 0.86). Females exercised less regularly (PR = 0.63, 95% C I = 0.51 – 0.76) than males. Participants with higher education exercised regularly (PR = 2.08, 95% CI = 1.45 – 2.99) than participants with lower education. Employment status was consistently associated with exercise and high levels of sedentary behavior. Participants who were not in full-time employment exercised more regularly (PR = 1.45, 95% CI = 1.1 – 1.92) and were less likely to report high levels of sedentary behavior (PR = 0.65, 95% CI = 0.44 – 0.97) than those in full-time employment.ConclusionsOur population-based study suggests a need to encourage overall physical activity but, particularly regular leisure-time exercise, especially among middle-aged, females and those with lower levels of education and full-time employment. Strategies targeting workplaces may be important to reduce high levels of sedentary behavior.

Hysteresis modeling of sequential application of orthogonal fields

In a cumulative effort to characterize the sizes, shapes, and distributions of the domains of the structurally superior high strength steels, a characteristic magnetization measurement and a Preisach model are presented to explain and model the distinctive trends observed in the data. The study investigates the effect of a constant transverse magnetic field and a cycling longitudinal magnetic field, applied sequentially, on the major hysteresis loop of solid cylinders of high strength steel. A coupled-hysteron vector Preisach model is extended to model the longitudinal magnetization of the samples under the effect of an applied transverse field. Insights about the microstructure of the rods and the contribution of different domains and magnetization mechanisms to the magnetic response are drawn.

Quality of Life in Older Age: Evidence from an Irish Cohort Study

To compare the role of different life domains in determining quality of life (QoL) in Ireland with international results. Cross-sectional study of responses to The Irish Longitudinal Study of Ageing. Individuals aged 50 and older living in private residential addresses in the Republic of Ireland. Eight thousand five hundred four individuals living in 6,279 households in the Republic of Ireland (response rate 62%) were interviewed and completed a self-completion questionnaire. Quality of life was measured using the Control, Autonomy, Self-realization and Pleasure measure (CASP-19). The domains of physical and mental health, social participation, economic resources, and sociodemographic status were measured using a large number of indicators. Ordinary least squares regression models were used to estimate the effect of factors on CASP-19, with the relative contribution of different domains to total explanation measured using a measure of statistical fit. The mean CASP-19 score for the sample was 43.8 (95% confidence interval = 43.6-44.1), significantly higher than the mean score for the English Longitudinal Study of Ageing (mean 42.5: 42.3-42.7). In unadjusted analyses, CASP-19 was curvilinear with age, peaking at 67 and falling thereafter. Controlling for predictors of QoL, CASP-19 continued to rise, at a decreasing rate, with age. Mental health independently explained the largest proportion of variance in CASP-19 (7.6%), but no single domain of life dominated in terms of explanation. Increasing longevity can be associated with increasing QoL as long as it is accompanied by reasonable levels of mental and physical health, high-quality relationships, and social participation. Even if physical health becomes poor, evidence suggests that QoL can often remain high as individuals find value and enjoyment in other dimensions of life.