Contracaecum Osculatum Research Articles

Diphyllobothrium sp. and Other Parasites of Migrating and Rare Fish Species in the Southern Baltic Sea and Coastal Waters, Poland.

The present study is the first to analyse the parasite fauna of sole Solea solea, dab Limanda limanda, hake Merluccius merluccius, whiting Merlangius merlangus, and plaice Pleuronectes platessa in the Pomeranian Bay, as well as saithe Pollachius virens from the Szczecin Lagoon (Poland). The aim of this study was to determine the occurrence of parasites in migrating and rare fish in the Pomeranian Bay and the Szczecin Lagoon and to determine the composition of the diet of these fish. The fish for analysis were obtained in the years 2010-2019. The typical marine nematode Capillaria (Procapillaria) gracilis, rarely recorded in Poland, was found, in addition to the following parasites: Ichthyophonus hoferi, Trichodina jadranica, Diphyllobothrium sp., Dichelyne (Cucullanellus) minutus, Raphidascaris acus, Anisakis simplex, Contracaecum osculatum, Hysterothylacium aduncum, Pseudoterranova decipiens, and Echinorhynchus gadi. Because the fish species analysed in the study are not typically present in the Baltic (with the exception of plaice), and because we do not know how long they feed while they are in the Baltic, we cannot be certain which parasites they acquired in the water bodies analysed in the study and which were introduced during the migration of fish. Although these fish are outside of their normal geographic range (except for plaice), in the new environment, there were enough suitable intermediate hosts for the parasites of these fish to complete their life cycle and survive.

De novo transcriptome assembly of an Antarctic nematode for the study of thermal adaptation in marine parasites

Understanding the genomic underpinnings of thermal adaptation is a hot topic in eco-evolutionary studies of parasites. Marine heteroxenous parasites have complex life cycles encompassing a free-living larval stage, an ectothermic intermediate host and a homeothermic definitive host, thus representing compelling systems for the study of thermal adaptation. The Antarctic anisakid Contracaecum osculatum sp. D is a marine parasite able to survive and thrive both at very cold and warm temperatures within the environment and its hosts. Here, a de novo transcriptome of C. osculatum sp. D was generated for the first time, by performing RNA-Seq experiments on a set of individuals exposed to temperatures experienced by the nematode during its life cycle. The analysis generated 425,954,724 reads, which were assembled and then annotated. The high-quality assembly was validated, achieving over 88% mapping against the transcriptome. The transcriptome of this parasite will represent a valuable genomic resource for future studies aimed at disentangling the genomic architecture of thermal tolerance and metabolic pathways related to temperature stress.

First record of Pseudoterranova decipiens in sprat (Sprattus sprattus) from the Baltic Sea.

A dispersion of Anisakidae nematodes (particularly Contracaecum osculatum) among marine organisms in the Baltic Sea has been reported over the last decade. This is in line with an increase in the number of grey seal that act as final host for Contracaecum osculatum and Pseudoterranova sp., and are thus indispensable for the completion of their life cycles. Most attention has been paid to zoonotic nematode species, like Pseudoterranova sp., which have been noted in commercially important fish in the area. Little is known about the spread and transmission of Pseudoterranova sp. in the Baltic Sea. The aim of this study was to investigate whether sprat may play a role as a transport host for this Anisakidae. Samples were collected in three areas of the southern Baltic Sea (south and east of Bornholm, Słupsk Farrow and the Gulf of Gdańsk) during a research cruise in August 2019. A visual inspection of the viscera of 556 sprats was conducted. Parasites were identified using anatomomorphological and molecular methods. Nematodes were recorded only in sprat caught southeast of Bornholm (prevalence 2.7%; intensity of infection 1-4; abundance 0.05). Molecular identification revealed the presence of Pseudoterranova decipiens. This is the first report of P. decipiens in sprat from the Baltic Sea. Sprat is likely a transmitter of P. decipiens in the Baltic Sea food web.

Seals, fish, humans and parasites in the Baltic: ecology, evolution and history.

Evolutionary and ecological processes affecting the interactions between hosts and parasites in the aquatic environment are at display in the Baltic Sea, a young and ecologically unstable marine ecosystem, where fluctuating abiotic and biotic factors affect the parasitofauna in fish. The dynamic infections of Baltic cod, a subpopulation of the Atlantic cod (Gadus morhua Linnaeus), with third stage anisakid nematode larvae of Pseudoterranova decipiens (Krabbe, 1878) and Contracaecum osculatum (Rudolphi, 1802) have increased following a significant increase of the Baltic grey seal Halichoerus grypus (Fabricius) population in the region. Cod serves as a paratenic host and marine mammals, pinnipeds, are definitive hosts releasing parasite eggs, with faeces, to the marine environment, where embryonation and hatching of the third stage larva take place. The parasite has no obligate intermediate hosts, but various invertebrates, smaller fish and cod act as paratenic hosts transmitting the infection to the seal. Contracaecum osculatum has an impact on the physiological performance of the cod, which optimises transmission of the larva from fish to seal. Thus, a muscle mass decrease of nearly 50% may result from heavy C. osculatum infections, probably amplified by a restricted food availability. The muscle atrophy is likely to reduce the escape reactions of the fish when meeting a foraging seal. In certain regions, where fish and seals are restricted in their migration patterns, such as the semi-enclosed Baltic Sea, the predation may contribute to a severe cod stock depletion. The parasites are zoonotic and represent a human health risk, when consumers ingest insufficiently heat- or freeze-treated infected products. Marked infections of the cod were previously reported during periods with elevated seal populations (late 19th and middle 20th century) and various scenarios for management of risk factors are evaluated in an evolutionary context.

Comparative histopathology of livers from Baltic cod infected with the parasitic nematode Contracaecum osculatum

Infection levels with the parasitic nematode Contracaecum osculatum in Eastern Baltic cod have increased in the last decades. Eastern Baltic cod is transport host for this parasite that has a high affinity for the liver of the fish. The liver is a highly vital organ and damage to the liver tissue can result in reduced functionality of the organ. Previous studies have revealed that cod with high infections loads reveal impaired physiological performance, reduced nutritional condition and show signs of having a liver disease. Yet, little is known about the pathological changes and inflammatory reactions of the cod liver related to the infections. In this study, we performed histological examinations on 30 Baltic cod livers caught in the eastern part of the Baltic Sea (length; 38 ± 0.9cm, weight; 454 ± 34.8gram) and three Sound cod livers (length; 63 ± 2.9cm, weight; 3396 ± 300.2gram) to categorize the degree of inflammation and its relation to pathological changes in infected cod livers. We further investigated how C.osculatum infection levels varied with intensity of inflammation and co-infections. We found that high infection loads with C.osculatum caused severe inflammation in the liver tissue of cod and reduced fat content of the hepatocytes. Conspicuous amounts of glycogen were found in the muscle and intestinal epithelial cells of the nematodes and parasitic co-infections occurred more frequently in the most heavily infected livers.

Contracaecum osculatum and Pseudoterranova sp. in the liver of salmon (Salmo salar) from Polish marine waters

Abstract Anisakidae nematodes, especially Contracaecum osculatum, Anisakis simplex, and Pseudoterranova decipiens, have dispersed throughout the Baltic Sea over the last decade. Despite the fact that salmon, Salmo salar, is a popular choice among consumers and therefore one of the most valuable Baltic fish species, information about the level of infection of salmon liver with these zoonotic nematodes is sparse. In 2020, a total of 120 salmon livers were inspected for the presence of parasites showing that 13% of salmon livers were infected with C. osculatum. Furthermore, a single Pseudoterranova sp. larva was detected in one salmon liver, representing a host-parasite system that has never previously been reported in the Baltic Sea.

Parasites of five vagrant Polar bears (Ursus maritimus) swimming to Iceland during 2008 to 2016

During 2008 to 2016, five polar bears (Ursus maritimus) swam from the East Greenland population to Iceland and were shot soon after walking ashore. Each bear was dissected. Ectoparasites were searched for in their ears and fur, helminths in the gastrointestinal tract and Trichinella larvae in muscles. Protozoan cysts, oocysts and helminth eggs were also searched for in faecal samples. No ectoparasites were detected. Two bears (40%) hosted fourth stage larvae of the nematode Contracaecum osculatum (strain B) in the stomach. Characteristic scars, noted as craters in the rectum wall, indicated previous acanthocephalan infection in one bear. Three polar bears were infected by the nematode Trichinella nativa. No protozoan parasites were detected in faecal samples, but cysts of the heterokontophyte Blastocystis sp. were found in faeces of two bears (40%). This is the first report of C. osculatum, Blastocystis sp. and an acanthocephalan infection in free-living polar bears. Keywords: Iceland, parasites, Ursus maritimus, polar bear, vagrant, new host records

Anisakid parasites (Nematoda: Anisakidae) in 3 commercially important gadid fish species from the southern Barents Sea, with emphasis on key infection drivers and spatial distribution within the hosts.

Northeast Arctic cod, saithe and haddock are among the most important fisheries resources in Europe, largely shipped to various continental markets. The present study aimed to map the presence and distribution of larvae of parasitic nematodes in the Anisakidae family which are of socioeconomic and public health concern. Fishes were sourced from commercial catches during winter or spring in the southern Barents Sea. Samples of fish were inspected for nematodes using the UV-press method while anisakid species identification relied on sequencing of the mtDNA cox2 gene. Anisakis simplex (s.s.) was the most prevalent and abundant anisakid recorded, occurring at high infection levels in the viscera and flesh of cod and saithe, while being less abundant in haddock. Contracaecum osculatum (s.l.) larvae, not found in the fish flesh, showed moderate-to-high prevalence in saithe, haddock and cod, respectively. Most Pseudoterranova spp. larvae occurred at low-to-moderate prevalence, and low abundance, in the viscera (Pseudoterranova bulbosa) and flesh (Pseudoterranova decipiens (s.s.) and Pseudoterranova krabbei) of cod, only 2 P. decipiens (s.s.) appeared in the flesh of saithe. Body length was the single most important host-related factor to predict overall abundance of anisakid larvae in the fish species. The spatial distribution of Anisakis larvae in the fish flesh showed much higher abundances in the belly flaps than in the dorsal fillet parts. Trimming of the flesh by removing the belly flaps would reduce larval presence in the fillets of these gadid fish species by 86–91%.

The effects of Contracaecum osculatum larvae on the growth of Atlantic cod (Gadus morhua).

Atlantic cod (Gadus morhua) from the Eastern Baltic stock have decreased in numbers and condition since the 1990′s. Among several causes, an increased prevalence and intensity of the nematode Contracaecum osculatum has been discussed. This increase has been attributed to a population increase of the parasites final host, the grey seal (Halichoerus grypus). Other studies have looked at the role of Contracaecum osculatum on cod growth and condition on recently caught cod, or done short term experimental studies in lab. This study instead investigated the importance of Contracaecum osculatum for cod growth in a sea pen based experiment, where cod were kept and fed in order to monitor growth. The results show that a higher density (number of nematodes per gram liver) decreases cod growth potential. If the number of nematodes exceeded 8 per gram liver cod did not grow in length, even when given generous amounts of food. Accounting for the lack of growth due to Contracaecum osculatum may improve stock assessments and increase the possibility to reach management targets.

Drug efficacy on zoonotic nematodes of the Anisakidae family: new metabolic data.

In the Anisakidae family, there are nematodes, most of which are parasitic for important commercial fish species. Both public health risks and socio-economic problems are attributed to these parasites. Despite these concerns, knowledge of the metabolism of these parasites remains unknown. Therefore, the main objective of this study was to investigate the receptors of drugs and oxidative metabolic status of two Anisakidae species, Pseudoterranova decipiens (s. s.) and Contracaecum osculatum (s. s.), under the influence of anthelminthic drugs, ivermectin (IVM) and pyrantel (PYR), at different concentrations: 1.56, 3.125 and 6.25 μg mL−1 of culture medium for 3, 6, 9, 12 and 72 h. The mRNA expressions of the γ-aminobutyric acid receptor, acetylcholine receptor subunits, adenosine triphosphate-binding cassette transporters and antioxidative enzymes were determined. The total antioxidant capacity and glutathione S-transferase activity were also examined. To the best of the authors' knowledge, this is the first time that IVM and PYR have been tested against these parasitic nematodes.

Absence of zoonotic parasites in salmonid aquaculture in Denmark: Causes and consequences

A parasitological investigation of Danish farmed freshwater (180 fish) and marine rainbow trout (170 fish), Oncorhynchus mykiss , was performed with an emphasis on the occurrence of zoonotic parasites. Reliability of the parasitological methods applied was assessed by examining fish from a stock of wild Atlantic cod Gadus morhua caught in the Sound (Øresund). While wild fish carried a number of zoonotic parasites, we found that farmed fish, both in the fresh and marine environment, were free of these parasites. Non-zoonotic trout parasites found were eye-flukes Diplostomum paracaudum, D. pseudospathaceum and Eubothrium crassum in maricultured rainbow trout. The acanthocephalan Echinorhynchus truttae and Diplostomum sp. were found in freshwater trout. In contrast, wild cod were infected with five different nematode species ( Anisakis simplex , Contracaecum osculatum , Pseudoterranova decipiens , Hysterothylacium aduncum , Cucullanus cirratus ), three trematode species ( Lepidapedon elongatum , Cryptocotyle lingua , Hemiurus communis ), one copepod species ( Lernaeocera branchialis ) and one myxozoan ( Myxobolus aeglefini ). The risk factors associated with aquaculture production in an infectious aquatic environment are analyzed and reasons for absence of zoonotic parasites in aquacultured trout are pinpointed. Based on the analysis recommendations for management practices securing parasite freedom in aquacultured fish are given. • Salmonid aquaculture in Denmark is free from zoonotic parasites. • Fish parasitological methods are validated by examination of wild fish. • Risk factors associated with aquaculture production are evaluated. • Recommendations for prevention of zoonotic parasite introduction are presented.

Feeding ecology and epizootological value of the Great Cormorant (<i>Phalacrocorax carbo</i>) during the secondary expansion of the Baikal region

Aim . To research the Great Cormorant ( Phalacrocorax carbo L., 1758) ecology during the secondary expansion of the Baikal region. Material and Methods . The material for studying cormorant nutrition was 28 stomachs of harvested birds, visual observations of feeding cormorants using optical equipment, as well as regurgitated burps of nutrition obtained by a bloodless method when being fed to chicks. 21 examples of cormorants were the subjects of the parasitological research. Results . The number of the species has been growing since the beginning of the XXI century and, starting from 2020/21, is likely to stabilize, remaining at a high level. This indicates the gradual integration of the species into the Baikal Lake ecosystem. The main food item of Cormorants are the non‐commercial fish, Sleeper ( Percottus glehni Dyb.), Perch ( Perca fluviatilis L.) and Roach ( Rutilus rutilus L.). Parasitological studies have revealed 8 species of helminths from three classes with varying degrees of invasion. Only one of them, Contracaecum osculatum baicalensis (Mosgovoy et Ryjikov, 1950), is endemic and potentially dangerous to human health (its infection rate can reach 80%). Conclusion . The Great Cormorant is an obligate, but not a specialized ichthyophage, focused on the prevailing fish species. Great Cormorants as hosts of ticks, helminths and other ecto‐ and endoparasites are potentially sources of threat to the occurrence and spread, primarily, of ornithoses and helminthiasis in the Baikal region and possibly of other diseases carried from their wintering places.

Nematodes in fish of genus Cottocomephorus (Cottidae)

The data on the morphology and relative abundance of nematodes in bottom-pelagic cottids Cottocomephorus grewingki and C. inermis met in Lake Baikal are presented. Three species of nematodes (Comephoronema werestschagini, Ichtiobronema hamulatum, Contracaecum osculatum baicalensis) were found. The differences in cottids invasion with nematodes are shown.

Parasite load of Atlantic cod Gadus morhua in the Baltic Sea assessed by the liver category method, and associations with infection density and critical condition

AbstractDuring the 2010s, Atlantic cod Gadus morhua L. in the eastern Baltic Sea experienced increasing infection loads of the parasitic nematode Contracaecum osculatum (Rudolphi) in their livers. Starting in 2021, a mandatory part of the routine sampling protocol on Baltic monitoring surveys is to assign a liver category to individual cod livers, based on the number of nematodes visible on the liver surface, to follow spatiotemporal changes in nematode infection loads. The validity of the liver category method has never been evaluated. Based on data from 642 cod livers, the method was verified and found to be a good predictor of the total number of nematodes. Moreover, the probability of cod being in a critical condition increased with the parasite load. In addition to their direct applicability to Baltic cod, the present findings may inspire others working with disease in fish stocks to include parasite monitoring.

Evidence of IgE-Mediated Cross-Reactions between Anisakis simplex and Contracaecum osculatum Proteins.

Fish consumers may develop allergic reactions following the ingestion of fish products containing nematode larvae within the genus Anisakis. Sensitized patients may cross-react with proteins from insects, mites and mollusks, leading to allergic reactions even in the absence of the offending food. Potential cross-reactivity in Anisakis-allergic patients with larval proteins from other zoonotic parasites present in freshwater and sea fish should be investigated due to an increasing occurrence in certain fish stocks, particularly Contracaecum osculatum. In this work, we evaluated IgE-cross reactions by in vivo (skin prick tests with parasites extracts) and in vitro methods (IgE-ELISA and IgE-immunoblot). In vivo skin prick tests (SPT) proved the reactivity of Anisakis-sensitized patients when exposed to C. osculatum antigens. Sera from Anisakis-sensitized patients confirmed the reaction with somatic antigens (SA) and excretory/secretory proteins (ES) from C. osculatum. Only anecdotal responses were obtained from other freshwater worm parasites. Consequently, it is suggested that Anisakis-sensitized humans, especially patients with high levels of specific anti-Anisakis antibodies, may react to C. osculatum proteins, possibly due to IgE-mediated cross-reactivity.

Review of metazoan parasites of the northern fur seal (Callorhinus ursinus) and the analysis of the gastrointestinal helminth community of the population on St. Paul Island, Alaska.

The northern fur seal (NFS), Callorhinus ursinus (Mammalia: Otariidae), is a marine mammal species included into the IUCN Red List as the vulnerable species which population is dramatically declining. A significant amount of parasitological data collected previously and our recent data allowed us to clarify the list of NFS metazoan parasites and to perform a comprehensive analysis of the gastrointestinal helminth community. Gastrointestinal tracts from 756 NFSs (3- to 4-year-old males) were collected during the annual Aleut subsistence harvests in July-August of 2011-2014 from five separate rookeries on St. Paul Island, Alaska. Totally, 27,625 specimens of helminths and approximately 1000 nasal mites were collected and identified. Detailed analysis of the previously published and newly obtained data revealed 32 species of metazoan parasites, including trematodes (6 species), cestodes (4), nematodes (9), acanthocephalans (9) and arthropods (4). The gastrointestinal helminth community of newly studied NFSs comprised 19 species including trematodes (4), cestodes (3), nematodes (5) and acanthocephalans (7). Temporal changes in the helminth community structure were small but statistically significant. Gastrointestinal helminth infracommunities comprised from 1 to 10 species (average of 4). Small but significant correlation was found between the abundances of acanthocephalans (Corynosoma similis and C. strumosum), nematodes (Contracaecum osculatum, Pseudoterranova spp.) and cestode Diphyllobothrium tetrapterum.

Proteomic Profiling Reveals New Insights into the Allergomes of Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum.

Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum third-stage larvae (L3) are fish-borne nematodes that can cause human anisakidosis. Although A. simplex is a known source of allergens, knowledge about the allergic potential of P. decipiens and C. osculatum is limited. Therefore, we performed comparative proteomic profiling of A. simplex, P. decipiens, and C. osculatum L3 larvae using liquid chromatography-tandem mass spectrometry. In total, 645, 397, and 261 proteins were detected in A. simplex, P. decipiens, and C. osculatum L3 larvae, respectively. Western blot analysis confirmed the cross-reactivity of anti-A. simplex immunoglobulin (Ig)G antibodies with protein extracts from P. decipiens and C. osculatum L3 larvae. The identified proteins of the Anisakidae proteomes were characterized by label-free quantification and functional analysis, and proteins involved in many essential biological mechanisms, such as parasite survival, were identified. In the proteome of A. simplex 14, the following allergens were identified: Ani s 1, Ani s 2 (2 isomers), Ani s 3 (2 isomers), Ani s 4, Ani s 8, Ani s 9, Ani s 10, Ani s 11-like, Ani s 13, Ani s fructose 1,6-bisphosphatase, Ani s phosphatidylethanolamine-binding protein (PEPB), and Thu a 3.0101. The following 8 allergens were detected in P. decipiens: Ani s 2, Ani s 3 (2 isomers), Ani s 5, Ani s 8, Ani s 9, Ani s PEPB, and Ani s troponin. In C. osculatum 4, the following allergens were identified: Ani s 2, Ani s 5, Ani s 13, and Asc l 3. Furthermore, 28 probable allergens were predicted in A. simplex and P. decipiens, whereas in C. osculatum, 25 possible allergens were identified. Among the putative allergens, heat shock proteins were most frequently detected, followed by paramyosin, peptidyl-prolyl cis-trans isomerase, enolase, and tropomyosin. We provide a new proteomic data set that could be beneficial for the discovery of biomarkers or drug target candidates. Furthermore, our findings showed that in addition to A. simplex, P. decipiens and C. osculatum should also be considered as potential sources of allergens that could lead to IgE-mediated hypersensitivity.

Contracaecum osculatum (sensu lato) infection of Gadus morhua in the Baltic Sea: inter- and intraspecific interactions

The subpopulation of Atlantic cod, Gadus morhua, in the eastern part of the Baltic Sea has experienced a significant increase in infections with anisakid nematode larvae of the species Contracaecum osculatum sensu lato (s.l.) since the year 2000. The life cycle of the parasite includes seals and especially the grey seal, Halichoerus grypus, as final hosts, carrying the adult nematodes in the stomach, crustaceans (copepods, amphipods) as first intermediate hosts and various fish species (clupeids, sandeel) including cod as second intermediate/paratenic hosts. Cod with a body length below 28 cm are generally non-infected but experience increasing infection levels when they switch to a piscine diet (infected intermediate/paratenic hosts). We present an overall frequency distribution analysis of worms in 166 cod (body length 30–49 cm) collected in the spawning area over the last 5 years. It shows a fit to the negative binomial distribution, a prevalence of infection of 89.8%, a mean intensity of 29.3 parasites per fish (range 1–377) and a variance/mean ratio of 59.2 (≫1), indicating overdispersion. We present measurements of the adult Contracaecum osculatum (s.l.) specimens in the seal stomach and show that the parasites reach a maximum length of 6.6 cm (females) and 5.8 cm (males). L3s in sprat have a total length from 1to 11 mm whereas the larvae in cod liver are 3–27 mm. A decreasing mean worm length associated with high worm densities in cod (number of nematodes per liver) was recorded. Possible explanations might include timing of feeding on infected intermediate/paratenic hosts, intraspecific competition (crowding) between larvae in cod and host responses (indicated by a significant antibody production in cod against C. osculatum (s.l.) antigens). A significant negative correlation between infection intensity and muscle mass of cod was found, suggesting parasite-induced down-regulation of growth factors in cod.

Anisakid nematode larvae in the liver of Atlantic cod Gadus morhua L. from West Greenland.

Anisakid nematode larvae occur frequently in the liver of Atlantic cod, but merely few infection data from cod in waters around Greenland exist. The present study reports the occurrence of third-stage anisakid larvae in the livers of 200 Atlantic cod caught on fishing grounds along the West coast of Greenland (fjord systems of Maniitsoq) in May, June, August and September 2017. Classical and molecular helminthological techniques were used to identify the nematodes. A total of 200 cod livers were examined, and 194 were infected with third-stage nematode larvae (overall prevalence of infection 97%) with a mean intensity of 10.3 (range between 1 and 44 parasites per fish). Prevalences recorded were 96% for Anisakis simplex (s.l.), 55% for Pseudoterranova decipiens (s.l.) and 8% for Contracaecum osculatum (s.l.). Sequencing the mtDNA cox2 from 8 out of 23 these latter larvae conferred these to C. osculatum sp. B. A clear seasonal variation was observed, with a rise in A. simplex (s.l.) and P. decipiens (s.l.) occurrence in June and August and a decline in September. The study may serve as a baseline for future investigations using the three anisakids as biological indicators in Greenland waters.

Nematode Parasites in Baltic Sea Mammals, Grey Seal (Halichoerus grypus (Fabricius, 1791)) and Harbour Porpoise (Phocoena phocoena (L.)), from the German Coast

PurposeEndoparasitic nematodes of six harbour porpoises Phocoena phocoena and four grey seals Halichoerus grypus, stranded at the eastern coast of the Baltic Sea in Germany in winter 2019, were analysed in order to identify nematode parasites and to compare with recent studies from the same area.MethodsEndoparasitic nematodes were identified by using both morphological and molecular characters. The successfully obtained sequences of the rDNA marker regions ITS-1, 5.8S, ITS-2 from 29 anisakid and the rDNA marker region ITS-2 of 11 pseudalid nematodes were amplified.ResultsAnalyses revealed the presence of three parasite species, the anisakid nematode Contracaecum osculatum from grey seals and the pseudalid nematodes Pseudalius inflexus and Stenurus minor from the harbour porpoises. Other anisakid nematodes regularly occurring in the Baltic Sea, e.g. Anisakis simplex or Pseudoterranova decipiens, were not found.ConclusionsThe prevalence of 100% and a severe parasite load in grey seals demonstrated a very high C. osculatum infection of Baltic Sea fish as their regular prey. Prevalence of 33% for parasites in harbour porpoises and minor infection rates, combined with a distinct lack of anisakid nematodes, are typical for the current situation of the porpoise parasite fauna in the Baltic Sea. Invasive parasite species as possible indicators for climate change could not be detected.