Mid-level vision is the intermediate visual processing stage for generating representations of shapes and partial geometries of objects. Our mechanistic understanding of these operations is limited, in part, by a lack of computational tools for analyzing image properties at these levels of representation. We introduce the Mid-Level Vision (MLV) Toolbox, an open-source software that automatically processes low- and mid-level contour features and perceptual grouping cues from real-world images. The MLV toolbox takes vectorized line drawings of scenes as input and extracts structural contour properties. We also include tools for contour detection and tracing for the automatic generation of vectorized line drawings from photographs. Various statistical properties of the contours are computed: the distributions of orientations, contour curvature, and contour lengths, as well as counts and types of contour junctions. The toolbox includes an efficient algorithm for computing the medial axis transform of contour drawings and photographs. Based on the medial axis transform, we compute several scores for local mirror symmetry, local parallelism, and local contour separation. All properties are summarized in histograms that can serve as input into statistical models to relate image properties to human behavioral measures, such as esthetic pleasure, memorability, affective processing, and scene categorization. In addition to measuring contour properties, we include functions for manipulating drawings by separating contours according to their statistical properties, randomly shifting contours, or rotating drawings behind a circular aperture. Finally, the MLV Toolbox offers visualization functions for contour orientations, lengths, curvature, junctions, and medial axis properties on computer-generated and artist-generated line drawings. We include artist-generated vectorized drawings of the Toronto Scenes image set, the International Affective Picture System, and the Snodgrass and Vanderwart object images, as well as automatically traced vectorized drawings of set architectural scenes and the Open Affective Standardized Image Set (OASIS).