Continuous-wave Beam Research Articles

High-throughput fluorescence lifetime imaging flow cytometry

Flow cytometry is a vital tool in biomedical research and laboratory medicine. However, its accuracy is often compromised by undesired fluctuations in fluorescence intensity. While fluorescence lifetime imaging microscopy (FLIM) bypasses this challenge as fluorescence lifetime remains unaffected by such fluctuations, the full integration of FLIM into flow cytometry has yet to be demonstrated due to speed limitations. Here we overcome the speed limitations in FLIM, thereby enabling high-throughput FLIM flow cytometry at a high rate of over 10,000 cells per second. This is made possible by using dual intensity-modulated continuous-wave beam arrays with complementary modulation frequency pairs for fluorophore excitation and acquiring fluorescence lifetime images of rapidly flowing cells. Moreover, our FLIM system distinguishes subpopulations in male rat glioma and captures dynamic changes in the cell nucleus induced by an anti-cancer drug. FLIM flow cytometry significantly enhances cellular analysis capabilities, providing detailed insights into cellular functions, interactions, and environments.

Terahertz fan-beam computed tomography.

A terahertz (THz) fan-beam computed tomography (CT) system using a 0.3 THz continuous-wave sheet beam is proposed. The diffraction-free sheet beam expands in a fan shape in only one direction and provides propagation-invariant focal lines and extended the depth-of-field. The fan-beam CT based on this beam is the second-generation THz CT. It breaks the conventional 4-f symmetric structure of THz CT using the parallel beam. The fan-beam THz CT allows for use with a linear array detector, which reduces the time required to collect data. To demonstrate its feasibility for three-dimensional (3D) imaging, the 3D structure of a metal rod packed in a carton is reconstructed with the support of the system. The results show that the object's internal structure can be obtained by this new THz CT system while retaining the geometrically magnified features of the cross-sectional structure. The results of our research provide a template for the second-generation THz CT system, which provides an additional method for nondestructive testing.

Selective CW Laser Synthesis of MoS2 and Mixture of MoS2 and MoO2 from (NH4)2MoS4 Film.

Very recently, the synthesis of 2D MoS2 and WS2 through pulsed laser-directed thermolysis can achieve wafer-scale and large-area structures, in ambient conditions. In this paper, we report the synthesis of MoS2 and MoS2 oxides from (NH4)2MoS4 film using a visible continuous-wave (CW) laser at 532 nm, instead of the infrared pulsed laser for the laser-directed thermolysis. The (NH4)2MoS4 film is prepared by dissolving its crystal powder in DI water, sonicating the solution, and dip-coating onto a glass slide. We observed a laser intensity threshold for the laser synthesis of MoS2, however, it occurred in a narrow laser intensity range. Above that range, a mixture of MoS2 and MoO2 is formed, which can be used for a memristor device, as demonstrated by other research groups. We did not observe a mixture of MoS2 and MoO3 in the laser thermolysis of (NH4)2MoS4. The laser synthesis of MoS2 in a line pattern is also achieved through laser scanning. Due to of the ease of CW beam steering and the fine control of laser intensities, this study can lead toward the CW laser-directed thermolysis of (NH4)2MoS4 film for the fast, non-vacuum, patternable, and wafer-scale synthesis of 2D MoS2.

Mid-infrared pulsed Er:ZBLAN fiber laser producing mode-switchable cylindrical vector beams.

We demonstrate the generation of both continuous-wave (CW) and Q-switched cylindrical vector beams (CVBs) from a mid-infrared Er3+-doped ZBLAN (Er:ZBLAN) fiber laser at ∼ 2.8 µm. A customized S-waveplate is incorporated as the intracavity mode converter to achieve the mid-infrared CVBs. Switchable modes of CVBs between the radially and azimuthally polarized beam can be realized easily by manipulating the cavity conditions. A maximum output power of ∼250 mW is achieved for the CW CVBs. In the short-pulsed CVBs operation regime, both the active and passive Q-switching modes are realized with a pulse duration of hundreds of nanoseconds. The proposed mid-infrared cylindrical vector lasers can have significant potential for applications in biomedicine, optical trapping, material processing and optical communication.

Superconducting radio frequency photoinjectors for CW-XFEL

A dependable and consistent electron source is a crucial requirement for the achievement of high-power free electron lasers (FELs). Over the past two decades, it has been demonstrated that photoinjectors based on SRF technology (SRF guns) are suitable for continuous wave (CW) beam generation. SRF guns possess both the high accelerating field gradients of normal conducting RF photoinjectors and the low power dissipation thanks to mature SRF cavity technology, and therefore have the potential to provide the high-brightness, high-current beams required for CW-XFELs. After the demonstration of the first SRF gun in Dresden-Rossendorf, several SRF gun programs based on different approaches have achieved promising progress and even succeeded in routine operation. SRF guns are expected to play an important role in XFEL facilities in the near future. In this paper, we give an overview of design concepts, important parameters and development status of the worldwide SRF gun projects.

Noncontact photoacoustic lipid imaging by remote sensing on first overtone of the C-H bond

Lipid imaging by conventional photoacoustic microscopy subjects to direct contact sensing with relatively low detection bandwidth and sensitivity, which induces superficial imaging depth and low signal-to-noise ratio (SNR) in practical imaging scenarios. Herein, we present a photoacoustic remote sensing microscopy for lipid distribution mapping in bio-tissue, featuring noncontact implementation, broad detection bandwidth, deep penetration depth, and high SNR. A tailored high-energy pulsed laser source with a spectrum centered at 1750 nm is used as the excitation beam, while a cofocused 1550 nm continuous-wave beam is used as the probe signal. The pump wavelength is selected to overlap the first overtone of the C-H bond in response to the intensive absorption of lipid molecules, which introduces a much-enhanced SNR (55 dB) onto photoacoustic remote sensing (PARS) signals. Meanwhile, the optical sensing scheme of the photoacoustic signals provides broadband detection compared to the acoustic transducer and refrains the bio-samples from direct contact operations by eliminating the ultrasonic coupling medium. Taking merits of the high detection sensitivity, deep penetration depth, broadband detection, and high resolution of the PARS system, high-quality tissue scale lipid imaging is demonstrated in a model organism and brain slice.

Innovative research and development activities on high energy and high current isochronous proton accelerator

The MW class proton accelerators are expected to play important roles in many fields, attracting institutions to continue researching and tackling key problems. The continuous wave (CW) isochronous accelerator obtains a high-power beam with higher energy efficiency, which is very attractive to many applications. Scholars generally believe that the energy limitation of the isochronous cyclotron is ∼1 GeV. To get higher beam power by the isochronous machine, enhancing the beam focusing become the most important issue.Adjusting the radial gradient of the average magnetic field makes the field distribution match the isochronism. When we adjust the radial gradient of the peak field, the first-order gradient is equivalent to the quadrupole field, the second-order, the hexapole field, and so on. Just like the synchrotron, there are quadrupoles, hexapole magnets, and so on, along the orbits to get higher energy, as all we know.If we adjust the radial gradient for the peak field of an FFA's FDF lattice and cooperate with the angular width (azimuth flutter) and spiral angle (edge focusing) of the traditional cyclotron pole, we can manipulate the working path in the tune diagram very flexibly. During enhancing the axial focusing, both the beam intensity and the energy of the isochronous accelerator are significantly increased. Here a 2 GeV CW FFA with 3 mA of average beam intensity design is presented. It is essentially an isochronous cyclotron although we use 10 FDF lattices. The key difficulty is that the magnetic field and each order of gradient should be accurately adjusted in a large radius range.As a high-power proton accelerator with high energy efficiency, we adopt high-temperature superconducting (HTS) technology for the magnets. 15 RF cavities with a Q value of 90000 provide energy gain per turn of ∼15 MeV to ensure the CW beam intensity reaches 3 mA. A 1:4 scale, 15-ton HTS magnet, and a 1:4 scale, 177 MHz cavity have been completed. The results of such R&D will also be presented in this paper.

Optical Guiding in 50-Meter-Scale Air Waveguides

The distant projection of high-peak and average-power laser beams in the atmosphere is a long-standing goal with a wide range of applications. Our early proof-of-principle experiments [Phys. Rev. X 4, 011027 (2014)] presented one solution to this problem, employing the energy deposition of femtosecond filaments in air to sculpt millisecond-lifetime sub-meter-length air waveguides. Here, we demonstrate air waveguiding at the 50-m scale, 60×longer, making many practical applications now possible. We employ a new method for filament energy deposition: multifilamentation of Laguerre-Gaussian LG01 “donut” modes. We first investigate the detailed physics of this scheme over a shorter 8-m in-lab propagation range corresponding to 13 Rayleigh lengths of the guided pulse. We then use these results to demonstrate optical guiding over 45 m in the hallway adjacent to the lab, corresponding to 70 Rayleigh lengths. Injection of a continuous-wave probe beam into these waveguides demonstrates very long lifetimes of tens of milliseconds.3 MoreReceived 9 August 2022Revised 30 November 2022Accepted 7 December 2022DOI:https://doi.org/10.1103/PhysRevX.13.011006Published by the American Physical Society under the terms of the Creative Commons Attribution 4.0 International license. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.Published by the American Physical SocietyPhysics Subject Headings (PhySH)Research AreasAngular momentum of lightLight-matter interactionNonlinear opticsOptics & lasersUltrafast opticsInterdisciplinary PhysicsAtomic, Molecular & Optical

Emission enhancement in laser-induced breakdown spectroscopy from sample heating with a continuous-wave diode laser

In this work an affordable, energy-efficient technique to enhance the sensitivity of the laser-induced breakdown spectroscopy technique is presented. This method is based in applying the beam of a continuous wave (CW) diode laser into the spot where the focused beam of a pulsed Nd:YAG, high-power laser induces the plasma. With the present set-up the CW beam heats the sample's surface to 300 °C. It was found that pre-heating the target in this way increased the line intensity of both neutrals and ions by a factor of up to 4.5. It was also found that the plasma excitation temperature and the electron density of the heated sample did not differ significantly from those at room temperature. However the amount of ablated material did change significantly when the sample was heated, with the crater in the latter case as much as 8.9 times larger than that obtained at ambient temperature. We conclude that the intensification in the pre-heated-sample case stems not from increased electronic plasma density or temperature but rather from a larger plume size resulting from an increased amount of ablated material when the target is heated.

Geometric spin Hall effect of a laser beam beyond the paraxial approximation

An intriguing spin-orbital angular momentum interaction, called the geometric spin Hall effect of light (GSHEL), has aroused much interest and manifests itself as a transverse shift. The GSHEL of the classical optical field is independent of the beam size. In this paper the GSHELs of the vortex vector continuous wave (VVCW) and vortex ultrashort pulsed beam (VUPB) are systematically analyzed beyond the paraxial approximation. The analytical expression of the transverse shift of the VVCW is obtained. Evidently, the magnitude of the transverse shift depends on the ratio of the beam waist to the wavelength. The transverse shift can be effectively enlarged by narrowing the beam waist even to the order of more than $10\phantom{\rule{4pt}{0ex}}\ensuremath{\mu}\text{m}$. This intriguing phenomenon is caused by the stronger spin-orbital interaction induced energy flow in the nonparaxial regime. Furthermore, the GSHEL of the VUPB is also explored. The pulse duration time strongly affects the distribution of the phase. The singularities play a crucial role in altering the orientation of the electric field. As a result, the VUPBs with different pulse duration time have different magnitudes of the GSHEL. These results may find significant applications in the investigation of the particle manipulation, microscopic imaging, and ultrashort optics.

Theoretical and experimental study of the overdense plasma generation in a miniaturized microwave ion source

To understand the plasma evolution mechanism of microwave ion source (MIS), a hybrid discharge heating (HDH) mode is proposed. That mode contains two parts: ignition discharge by surface wave plasma (SWP) and ionization by electron cyclotron resonance. Compared with the traditional electron cyclotron heating (ECH) mode, the HDH mode has a wider scope of application for MIS with a chamber diameter smaller than the cutoff size. The spatio-temporal evolution of electric field, power deposition, electron temperature, and electron density of a miniaturized microwave ion source (MMIS) at Peking University is investigated based on the HDH mode. In addition, the MMIS is optimized based on the theoretical results of the HDH mechanism. Preliminary experiments show that a mixed hydrogen continuous wave beam of up to 25 mA at 30 keV can be extracted with a power efficiency of 25 mA/100 W.

Seven-channel all-optical reconfigurable canonical logic units multicasting at 40 Gb/s based on a nonlinearity-enhanced silicon waveguide

All-optical canonical logic unit (CLU) is the basic building block of high-speed optical logic operation and complex optical computing. By utilizing the parallelism of optical signals, multichannel multicasting of all-optical CLUs can expand the capacity of the computing system effectively. Here, we propose and experimentally demonstrate the 40 Gb/s all-optical reconfigurable two-input CLUs generated in seven wavelength channels via four-wave mixing (FWM) in the nonlinearity-enhanced silicon waveguide. By introducing reverse-biased PIN junctions to reduce nonlinear loss, the output power of converted light can be increased over 10 dB. Moreover, pumped by two optical signals and a continuous wave beam, a full set of reconfigurable CLUs is multicasted in seven parallel wavelength channels. All logic signals with error-free performance are realized. Attributing to the rate transparency of FWM and parallel multicasting of logic functions, the proposed scheme offers more flexibility and expandability in future high-speed optical logic processing and complex optical computing.

Beam dynamics design of HIAF RFQ

The high-intensity heavy ion accelerator facility is a next-generation advanced heavy-ion accelerator facility built by the Institute of Modern Physics, Chinese Academy of Sciences. The RFQ is designed to provide a continuous wave beam and 2[Formula: see text]mA pulse beam with high-quality longitudinal beam distribution for the injection linear accelerator. Two different designs of aiming to suppress the longitudinal emittance were studied, and the optimized scheme which composed of a three-harmonic pre-buncher and an RFQ accelerator with small longitudinal acceptance was chosen. More emphasis is put on the section between pre-buncher and RFQ, where the space charge effect becomes severe with bunched beam. The optimal design and the analysis are presented in this paper.

Anti-resonant hollow-core fiber fusion spliced to laser gain fiber for high-power beam delivery.

We present the selective excitation of the fundamental mode in an anti-resonant hollow-core fiber (ARHCF) fusion-spliced with a commercial large mode area (LMA) fiber. By designing and fabricating a single-ring ARHCF that is mode-matched to a LMA fiber and by splicing the two using a CO2 laser-based splicer, we achieve a coupling efficiency of 91.2% into the fundamental mode. We also demonstrate an all-fiber integration of an ARHCF with a commercial ytterbium-doped fiber in a laser cavity for beam delivery application. Coupling of the single-mode laser output beam into the fundamental mode of the ARHCF is demonstrated with 90.4% efficiency (<0.45dB loss) for up to 50 W continuous wave beam in a stable and alignment-free all-fiber laser setup.

Thermal and non-thermal intensity dependent optical nonlinearities in ethanol at 800 nm, 1480 nm, and 1560 nm

Intensity dependent self-action of a continuous wave (CW) or pulsed optical beam can lead to spatial or spectral effects upon propagation through a nonlinear medium, which can be described as an intensity dependence of the refractive index, known as self-phase modulation (SPM). In this work, we revisit the nonlinear optical propagation of a CW and a CW mode-locked (CW-ML) high repetition rate [ ∼ megahertz (MHz)] laser propagating through pure ethanol in regions of very low optical absorption (800 nm) or very high absorption (1480 nm, 1560 nm). Spatial and spectral SPM and Z -scan experiments were performed to clarify the origin of the third-order nonlinear optical response in the different optical excitation regimes. From spatial SPM and Z -scan at either CW or CW-ML MHz regime, a thermal nonlinear response was determined to be the origin of the nonlinearity at 800 nm or 1480 nm, 1560 nm region, with the nonlinear refractive index response of the order of 10 − 4 − 10 − 9 c m 2 / W . From the spectral SPM, the non-thermal origin of the nonlinearity, arising from electronic or nuclear processes in the ethanol, was determined, and values of the order of 10 − 13 − 10 − 16 c m 2 / W were obtained, depending upon the spectral region. The results were supported by theoretical calculations using the nonlinear Schrödinger equation for the spectral behavior or Fresnel–Kirchhoff integral for the spatial results and clarify some of the misinterpreted results reported in the literature, besides complementing other nonlinear refraction data available at different wavelengths from 355 nm to 1560 nm.

Long lifetime of bialkali photocathodes operating in high gradient superconducting radio frequency gun

High brightness, high charge electron beams are critical for a number of advanced accelerator applications. The initial emittance of the electron beam, which is determined by the mean transverse energy (MTE) and laser spot size, is one of the most important parameters determining the beam quality. The bialkali photocathodes illuminated by a visible laser have the advantages of high quantum efficiency (QE) and low MTE. Furthermore, Superconducting Radio Frequency (SRF) guns can operate in the continuous wave (CW) mode at high accelerating gradients, e.g. with significant reduction of the laser spot size at the photocathode. Combining the bialkali photocathode with the SRF gun enables generation of high charge, high brightness, and possibly high average current electron beams. However, integrating the high QE semiconductor photocathode into the SRF guns has been challenging. In this article, we report on the development of bialkali photocathodes for successful operation in the SRF gun with months-long lifetime while delivering CW beams with nano-coulomb charge per bunch. This achievement opens a new era for high charge, high brightness CW electron beams.

Single Molecule Flourescence Methods to Monitor Site-Specific Fluctuations of Cy3 Monomer and Dimer Labeled DNA Constructs within Macromolecular Machines

Local fluctuations of the sugar-phosphate backbones of DNA (a form of DNA ‘breathing’) play key roles in protein-DNA assembly and enzymatic function. By monitoring spectroscopic signals from single-molecules of DNA constructs labeled with optical probes that are either rigidly inserted into the sugar-phosphate backbones or attached using flexible linkers, it is possible to study local conformational fluctuations within DNA at specific sites, and the dependence of these fluctuations on base sequence and secondary structure. Here we present two experimental single molecule spectroscopic methods, recently developed in our laboratory, to monitor the local fluctuations of Cy3-labeled DNA constructs. Both methods combine single-molecule total internal reflection fluorescence (TIRF) microscopy with polarized, phase-modulated optical excitation to detect, alternatively, linear and nonlinear optical signals. In Method 1 we use a linearly polarized continuous wave (cw) beam to excite the sample, so that the emitted fluorescence contains information about the rotational motion of a Cy3 monomer labeled DNA construct, or alternatively, the relative conformational changes of a Cy3 dimer inserted into the DNA sugar-phosphate backbones. In Method 2, we use sequences of broadband optical pulses to excite the sample, so that the ensuing fluorescence contains information about the linear absorbance spectrum, and alternatively, the two-dimensional fluorescence spectrum (2DFS). The latter experiments can be used to monitor the details of local conformational changes of a Cy3 dimer labeled DNA construct. Together, the two methods can be used to study biomolecular dynamics with varying levels of time resolution and structural information. For example, Method 1 can be applied to study sub-millisecond equilibrium fluctuations of Cy3 labeled DNA constructs, while Method 2 is well suited to monitor conformational transitions of a Cy3 labeled DNA construct during a biochemical reaction.

Laser induced broad band white emission from transparent Cr4+:YAG ceramics: Origin of broadband emission

Laser-induced white (light) emission was observed from transparent Cr4+:YAG ceramics irradiated with a focused continuous wave beam of light from an infrared laser diode. The laser-induced white emission is detected only on the surface of the sample and is not observed in volume. It is found that the intensity of the emission increases exponentially with the laser power density above the threshold. The impact of broadband emission on the power of the transmitted laser beam through the sample was measured. The disappearance of broadband emission due to displacement of the laser beam or an increase in ambient pressure leads to a decrease in the power of transmitted laser beam. Origins of the laser-induced white light emission along with its characteristic features are discussed in terms of multiphoton absorption, intervalence charge transfer and ionic space charge models.

Investigating the Nonlinear Optical Response of Pepper Oil under Low Power Irradiation with Continuous Wave Visible Laser Beam

The nonlinear optical properties of pepper oil are studied by diffraction ring patterns and Z-scan techniques with continuous wave beam from solid state laser at 473 nm wavelength. The nonlinear refractive index of the sample is calculated by both techniques. The sample show high nonlinear refractive index. Based on Fresnel-Kirchhoff diffraction integral, the far-field intensity distributions of ring patterns have been calculated. It is found that the experimental results are in good agreement with the theoretical results. Also the optical limiting property of pepper oil is reported. The results obtained in this study prove that the pepper oil has applications in nonlinear optical devices.

Optical beaming of electrical discharges

Igniting and guiding electrical discharges to desired targets in the ambient atmosphere have been a subject of intense research efforts for decades. Ability to control discharge and its propagation can pave the way to a broad range of applications from nanofabrication and plasma medicine to monitoring of atmospheric pollution and, ultimately, taming lightning strikes. Numerous experiments utilizing powerful pulsed lasers with peak-intensity above air photoionization and photo-dissociation have demonstrated excitation and confinement of plasma tracks in the wakes of laser field. Here, we propose and demonstrate an efficient approach for triggering, trapping and guiding electrical discharges in air. It is based on the use of a low-power continuous-wave vortex beam that traps and transports light-absorbing particles in mid-air. We demonstrate a 30% decrease in discharge threshold mediated by optically trapped graphene microparticles with the use of a laser beam of a few hundred milliwatts of power. Our demonstration may pave the way to guiding electrical discharges along arbitrary paths.