NO3 Content Research Articles

Balance of Nitrate and Ammonium in Tropical Soil Conditions: Soil Factors Analyzed by Machine Learning

The nitrogen/N dynamic is complex and affected by soil management (i.e., residue accumulation and correction/fertilization). In soil, most of the N is combined with organic matter (organic forms), but the N forms absorbed by plants are ammonium/NH4+ and nitrate/NO3− (inorganic forms). The N recommendation for agriculture crops does not observe the N available in the soil (organic or inorganic), indicating a low efficiency in nitrogen management in soil. Based on the hypothesis that the stocks of NO3− and NH4 can be used as indicative of N status in soil but with high variation according to soil factors (soil uses and management), the objective of the study was to (i) analyze the balance of nitrate and ammonium in tropical soil with different uses and management and (ii) use machine learning to explain the nitrogen dynamic in soil and the balance of nitrate and ammonium. The results showed that soil N stocks and pH promoted the formation of three clusters with the similarity between Cluster 1 (clay texture) and Cluster 2 (loam texture), represented by higher contents of nitrate as a result of high nitrification rate and lower contents of ammonium in soil. Cluster 3 (sand texture) was isolated with different N dynamics in the soil. In agricultural soils, the content of NO3− tends to be higher than the content of NH4+. There is a high nitrification rate in clay soil explained by higher organic matter and clay content that promotes soil biology. Based on the results of machine learning, for clay and loam soil, the contents of NO3 can be used as indicative of N status as a final result of nitrification rate and higher variation in soil. However, in sandy soil, NO3 can not be used as indicative of N status due to N losses by leaching.

Balancing river water pollution and agricultural development: A tradeoff threshold approach

Balancing environmental protection and social-economic development in agricultural land use management is a dilemma for decision-makers. Based on the modelling of the impacts of land use changes on river water pollution by SWAT model, the tradeoff between tea plantation expansion and river water quality was detected. SWAT model performs well in simulating the non-point source (NPS) pollution in agricultural watershed. The results showed that the tea plantation area expanded dramatically from 44 km2 in 2000 to 169 km2 in 2020 at the high cost of forest land. Consequently, the mean contents of NO3−-N and TN have significantly increased by 100% and 91% respectively in the past 20 years. And the NO3−-N in river water accounted for over 80% of TN in the tea plantation area. The NO3−-N and TN concentrations were positively related with the proportions of tea plantation area (Tea%) at different periods. The high pollution levels of NO3−-N and TN are priority control targets for river water quality management. The results indicated that the proportion of tea plantation thresholds lead to abrupt changes in river water quality. When the Tea% exceeded 3.0% in 2000, the probability of N pollution increased sharply. Whereas in 2020, it is suggested that the Tea% should not exceeds 18% to avoid sudden deterioration of water quality. The critical interval value of the Tea% for sudden change in N pollution showed an obvious increase tendency. The accelerating of nutrient pollution in rivers reduced the sensitivity of water quality to tea plantation expansion. Our results can provide new insights and empirical evidence for balancing the tradeoff between agricultural development and river water quality protection by demonstrating the carrying capacity threshold of river water environment for the expansion tea plantation.

Seasonal Dynamic of NO3− and K+ in a Citrus Crop Irrigated by Different Water Qualities

This study evaluated the effect of (i) irrigation water source: transfer (TW) and reclaimed water (RW), and (ii) crop phenological stage: winter rest (WR), flowering-sprouting (FS), and fruit growth (FG), on NO3− and K+ dynamics in soil and leaf of a citrus crop. The experiment was carried out during the 2018 and 2019 growing seasons on adult ‘Star Ruby’ grapefruit trees (Citrus paradisi Macf.). The concentration of both nutrients was periodically measured in soil and leaf samples and continuously monitored in the soil soluble fraction with nutrient sensors. Moreover, soil NO3− leaching was indirectly estimated by the periodic measurement of the leaf enrichment in 15N isotope (15Nleaf). The two water sources showed a different nutrient loading. Thus, NO3− and K+, were approximately 5 and 7 times higher, respectively, in the RW. Furthermore, the average contents of NO3− and K+ in the soil samples from the RW treatment were 10.1 and 19.7%, respectively, higher than in TW, with the highest soil NO3− leaching observed in RW treatment. In line with this, the mean contents of NO3− and K+ in the leaves from the RW treatment were 106.9 and 30.4% higher than the TW ones. As for the different phenological stages, in the FG stage, the lowest concentrations of NO3− in the soil samples and the highest in the leaf tissue were observed after a high soil leaching event. In this study the nutrient sensors measurements varied according to the dynamic of NO3− and K+ in the soil samples. The use of RW promoted an accumulation of NO3− and K+ in the soil and leaves of grapefruit trees, but also enhanced soil NO3− leaching, indicating that the proper management of this water source is necessary to avoid soil contamination. The mobilization of NO3− and K+ from soil to leaf was the highest in the FG stage, to ensure fruit development and vegetative growth.

Evaluation of Knowledge and Awareness of Dietary Nitrate Among Clinical Dietitians in Jeddah, Saudi Arabia: A Cross-Sectional Study.

Background and objectives Dietary nitrate (NO3) plays an important role in human physiological processes.In the past, inorganic NO3was viewed negatively due to its link with carcinogenic effects, notably nitrosamine formation in the stomach; yet, current perspectives acknowledge NO3 as a potentially beneficial dietary element. Nutrition professionals (NPs) are crucial in promoting NO3 awareness in health and academic settings. The study aimed toevaluate the knowledge of NPs in Jeddah,Saudi Arabia, regarding the biological roles of dietary NO3, taking into consideration their qualifications and years of experience. Methods A cross-sectional study was conducted among NPs who had graduated from clinical nutrition programs or were employed in clinical or academic settings. A validated 12-item online questionnaire was used to assess dietary NO3 knowledge across five areas: health effects, dietary sources, recommendations, biomarkers of intake, andmetabolism. The nitrate knowledge index (NKI) score was used to evaluate responses. Results Eighty-nine female NPs out of 144 completed the questionnaire. Most were ≤30 years old (75.4%) and had an undergraduate degree in clinical nutrition (70.8%), but 37 of them had ≤3 years of experience (62.7%). Overall, poor knowledge scores were observed among NPs, with a median (25th and 75th percentile) score of 10 (6, 13) out of 23. The majority (64%) perceived NO3 to be beneficial. However, most of the participants did not know its benefits in lowering blood pressure (BP) (68.5%) and were unsure about the effects of nitrate on cognitive function (60.7%) or kidney function (57.3%). Almost half of the NPs were unaware of NO3 sources and unsure about the mechanisms of the conversion of NO3into nitrogen dioxide(NO2) in the mouth (48.3%). Overall, knowledge of factors that affect NO3 content in food was good. No significant differences were observed in the median NKI scores among the participants based on their level of education or years of experience. Conclusion This study suggests NPs lack knowledge about dietary NO3.To address this, educational programs should be developed and implemented in clinical and academic settings.

Effects of Vermicomposting on Compost Quality and Heavy Metals：A Meta-analysis

In order to comprehensively evaluate the effects of vermicomposting on compost quality and the conversion of heavy metals under different control conditions, 109 studies were reviewed. The effects of earthworm species, pre-compost time, ventilation methods, initial C/N, initial pH, and initial moisture of the raw materials on compost quality and the heavy metal toxicity were quantitatively discussed during the vermicomposting process through Meta-analysis. The results showed that the six subgroups of factors all showed obvious influences on the compost quality and heavy metal toxicity. After vermicomposting, the contents of NO3--N (116.2%), TN (29.1%), TP (31.2%), and TK (15.0%) were significantly increased, whereas NH4+-N (-14.8%) and C/N (-36.3%) were significantly decreased. Meanwhile, the total amount of Cu and Cr of the final compost and their bioavailability were significantly reduced. Considering the influences of grouping factors on compost quality and heavy metals, it is recommended to adjust the initial moisture of pile materials to 70%-80%, C/N to 30-85, and pH to 6-7 and to conduct pre-composting for 0-15 d; additionally, vermicomposting should be naturally placed when the composting is aimed at promoting the compost quality. If the main purpose is to weaken the perniciousness of heavy metals in the raw material, it is recommended to adjust the initial moisture of the material to 50%-60%, C/N to less than 30, and pH to 7-8; to conduct no pre-compost; regularly turn the piles; and use the earthworm Eudrilus eugeniae for vermicomposting.

Impact of Pre-Plant Fertilizer Rates in Combination with Polysulphate® on Soil Nitrogen Distribution and Yield of Short-Day Strawberries (Fragaria × ananassa cv. Camarosa)

Strawberries are by far the most produced soft fruit (blueberries, raspberries, blackberries, and strawberries) worldwide, with China and the US being the two countries with the most production. In the US, strawberries reached a farm gate value of more than USD 3 billion in 2023 and are predominantly grown in the open field on an annual cycle in hilled-up soil beds using plastic mulch (plasticulture). This process relies on adequate pre-plant fertilizer application for plant establishment and fruit development. In North Carolina (US), it is current practice to apply pre-plant fertilizer containing 67 nitrogen (N) kg/ha; however, with increasing fertilizer costs and environmental concerns, questions remain as to whether or not pre-plant full-spectrum fertilizer rates can be reduced and substituted with organic low-N fertilizer sources, such as Polysulphate, without impacting yield or fruit quality. For this reason, field trials were established to evaluate the impact of pre-plant fertilizer rates on strawberry production (‘Camarosa’). Trials were conducted in the 2019–2020 and 2020–2021 seasons at two locations in North Carolina. The following N-rate pre-plant fertilizer treatments were applied: 80.1, 67.3, 54.1, 41.1, 33.6, 28.0, and 0 kg/ha. NO3 and NH4 content were frequently assessed in each replicate in the raised bed profile between planting and spring fertilizer application. Marketable and total strawberry yields were assessed over 6 weeks in the spring of 2020 and 2021. Our trial results showed that, especially in sandy soil, N rapidly declined under plastic within the first 8 weeks after pre-plant fertilizer application. However, no impact between pre-plant fertilizer rates and plant yield was observed. Treatments that contained Polysulphate and lower amounts of full-spectrum fertilizer showed significantly lower N concentrations in the soil while maintaining similar yields and fruit quality compared to grower-standard pre-plant fertilizer treatments. In summary, our results show that it is possible to reduce N content in pre-plant fertilizer by up to 50% compared to current recommendations without causing yield loss in short-day strawberry cultivars.

Changes in the Soil Microbial Community Structure and Driving Factors during Post-Fire Recovery of the Larix gmelinii Rupr. Forest in Northern China

Fire is crucial for shaping northern forest ecosystems and can affect soil microbial community structure. However, there are few studies on the long-term effects of forest fire disturbance on soil microbial community diversity. In this study, we employed high-throughput sequencing of 16S rRNA and ITS1 to assess variations in the abundance of bacterial and fungal communities in dominant populations at 1, 6, and 11 years post-fire. Furthermore, a comprehensive analysis was conducted to examine the relationship between soil microenvironmental changes and soil microbial communities after fire disturbances, considering soil physicochemical properties, including bulk density, moisture content, pH, organic carbon, total nitrogen, ammonium nitrogen, nitrate nitrogen, available potassium, and available phosphorus. We found that fire significantly increased soil pH, NO3−-N, AP, and AK contents, in which the content of NO3−-N basically recovered to the pre-fire level at 11 years after fire. The soil SOC and TN contents decreased significantly 1 year after the fire. However, compared to the unfired site, the SOC content essentially recovered 11 years after the fire, while TN content was still significantly higher 11 years after fire. Furthermore, fire changed the diversity and richness of soil microbial communities to some extent. PCoA and NMDS analyses suggested that the bacterial community structures in soil samples from different burned areas with different recovery periods exhibited similarity. However, notable differences were observed in the fungal community structures between the 1-year and 6-year post-fire study sites when compared to the unburned control site. Bacterial communities predominantly comprised Proteobacteria, Actinobacteria, and Acidobacteria, while fungal communities were mainly dominated by Ascomycota and Basidiomycota. RDA confirmed the significant roles of SOC, TN, and NO3−-N in affecting the diversity of soil microbial communities. Therefore, our study not only enhances our understanding of the long-term effects of forest fire disturbances on soil properties and soil microbial community structure, but also provides insights for further utilizing and controlling carbon and nitrogen content to regulate soil microbial activity and accelerate the recovery process of burned areas.

Soil Nitrogen Distribution Affects Nitrogen Utilization and Yield of Drip-Irrigated Rice

The cultivation of drip-irrigated rice has resulted in lower yields. However, the decrease in rice yield under drip irrigation and its relationship with the existing water and N regime have not been fully explained. Research and development of optimized water and N-management techniques are crucial for increasing rice yield under drip irrigation. In this study, two irrigation treatments were set: conventional drip irrigation (DIO) and drip irrigation with water stress (DIS). Each irrigation treatment contained four N rates: urea N 240 kg ha−1 (LN), urea N 300 kg ha−1 (MN), urea N 360 kg ha−1 (HN), and ammonium sulfate N 300 kg ha−1 (AN). The soil’s ammonium and nitrate contents were measured on the 2nd and 28th days after N application at panicle initiation stage. At anthesis, the aboveground and root biomass of rice were measured. In heading and maturity stage the N content of aboveground was measured and the yield, yield components, and NPFP were assessed at maturity stage. The results showed the following: (1) On the second day after N application, the contents of soil NO3−-N and NH4+-N in the 0–10 cm soil layer were highest for both the DIO and DIS. On the 28th day after N application, the soil NO3−-N content was highest at the 20–40 cm depth, while the soil NH4+-N content was still highest at the 0–10 cm depth. (2) The aboveground and root biomass in DIO treatment were significantly higher than in DIS. Furthermore, the root biomass at the 0–10 cm depth was significantly greater than at the 10–50 cm depth for both the DIO and DIS treatments. In the DIO treatment, the root biomass at the 10–50 cm depth was significantly higher with the HN and AN treatments compared to MN. However, in the DIS treatment, the root biomass at the 10–50 cm depth did not show significant differences between the MN, HN, and AN. (3) N accumulation in rice was significantly higher for the DIO treatment compared to the DIS treatment. Under the same irrigation treatment, the N accumulation in rice was highest in the AN and lowest in the LN. The PrNTA and PrNTC in DIS were significantly higher than in DIO, while the PoNAA and PoNAC were significantly lower in DIS. (4) The number of panicles, spikelets per panicle, seed-setting rate, 1000-grain weight, and grain yield were significantly lower in DIS. Under the DIS, these parameters were not significantly different among the MN, HN, and AN. In the DIO, the seed-setting rate, 1000-grain weight, and yield were not significantly different between the HN and AN, but were significantly higher than in the MN and LN. (5) NPFP was significantly higher in the DIO compared to the DIS. Among the different N rates, NPFP was highest with the AN treatment and lowest with the LN. In summary, under drip irrigation, there was a mismatch between soil mineral N and the distribution of rice roots, leading to reduced N accumulation and utilization in rice, ultimately impacting yield formation. Increasing N application and soil ammonium nutrition can improve rice yield under drip irrigation. However, optimizing N fertilizer management may not increase rice yield further when irrigation is further limited.

Effects of Experimental Nitrogen Deposition and Litter Manipulation on Soil Organic Components and Enzyme Activity of Latosol in Tropical Rubber Plantations

It is of great scientific significance in regulating plantation ecosystem restoration to investigate the effects of the nitrogen (N) deposition and litter manipulation on soil organic carbon components and enzyme activities. A micro-plot experiment was conducted with four nitrogen additions[CK (0 kg·hm-2·a-1, calculated by N), LN (50 kg·hm-2·a-1), MN (100 kg·hm-2·a-1), and HN (200 kg·hm-2·a-1)] and two litter treatments[LR (litter removal) and L (litter retained)] for tropical rubber plantations in western Hainan Island. The soil physico-chemical properties, soil organic carbon components, and enzyme activities in 0-10 cm and 10-20 cm depths were analyzed. The results showed that soil pH significantly decreased with elevated N addition and litter removal. The contents of NO3--N and NH4+-N significantly increased with elevated N addition. Moreover, there was a significant interaction between N addition and litter treatment on the contents of NO3--N and NH4+-N (P < 0.05). Compared to that with L, LR reduced SOC and its component contents; particularly, the largest decrease was in LFOC by 29.0%-81.4% in the 0-10 cm depth and 23.5%-58.4% in 10-20 cm, respectively. The contents of SOC and its components presented a trend of increasing first and then decreasing with elevated N addition irrespective of litter treatment, and those contents were significantly higher at LN than those at HN. There was a significant interaction between N addition and litter treatment on SOC, LFOC (0-10 cm), and HFOC contents. Compared with that under L, PPO activity was significantly reduced at LR under CK and LN but was significantly increased at LR under MN and HN, respectively. Variance analysis showed significant interactive effects between N addition and litter treatment on PPO and CBH (0-10 cm) activities, and the soil enzyme activity (BG, PPO, and CBH) responding to N addition was greater than that to the litter treatment. Pearson correlation analysis showed that SOC content was extremely positively correlated with MBC, POC, LFOC, and HFOC contents. To summarize, litter retained combined with low N deposition played an important synergistic role of improving SOC pool and soil enzyme activities for tropical rubber plantation systems.

EFFECT OF THE FORM OF ELECTRIC DISCHARGE ON PHYSICOCHEMICAL PROPERTIES OF PLASMA-ACTIVATED DISTILLED AND GROUND WATERS

A comparative study of some physicochemical properties of aqueous solutions resulted from the treatment of distilled and ground waters by pulsed discharges has been performed. Changes in the pH value, the conductivity of the solution, and the content of NO3¯ anions and Ca++, Mg++, K+, and Na+ cations were studied. Water bodies were affected by active particles formed in the air under the action of a pulsed discharge, which is implemented in two options. The first option is conventionally called ‘bubble discharge’ since it assumes the ignition of a discharge in an air bubble in direct contact with a liquid. The second option is the case when active particles, which are formed in an air stream under the action of a barrier discharge, are blown into a liquid. It is shown that the bubble discharge in ground water gives the maximum performance for the NO3¯ anions, while in the case of barrier discharge the best performance in the formation of NO3¯ anions is observed in distilled water. The pH value for ground water increases from 8.1 to 8.6 in the course of a 10-minute treatment using both discharges. However, in the case of distilled water treatment an inverse relationship is observed, so its value decreases from 7.5 to 3.5 pH. A multiple increase in the content of Ca++ and Mg++ cations in the water sample is observed when groundwater treating. Presumably, their increase in solution is associated with the transition of insoluble salts of calcium and magnesium carbonates (constant water hardness) into water-soluble nitrates (temporary hardness). In addition to magnesium and calcium ions, potassium and sodium cations were monitored in water samples. Their concentration in water was insignificantly affected by the treatments with discharges.

INFLUENCE OF SHELTERBELTS OF DIFFERENT DESIGNS ON SOIL FERTILITY AND MOISTURE AVAILABILITY IN THE RIGHT-BANK FOREST-STEPPE

During 2020-2023, the authors studied nutrient reserves, namely the content of humus, mobile nitrogen, phosphorus and potassium, as well as the level of acidity in the soil layers of 0-20 and 20-50 cm in openwork-blowing, openwork, dense forest strips, determined the reserves of total and available moisture, the dynamics of productive moisture and moisture availability during the growing season in individual collective farms in the Uman district of Cherkasy region. Studies have shown that the humus content in forest strips of various designs ranges from 2.5 to 3.6%. That is, the humus content is low. The highest amount of humus was found in the openwork design of the forest belt. The pH level in the 0-20 cm soil layer ranges from 5.87 in the openwork-blown forest belt to 7.3 units in the dense one. The highest NO3 content is observed in the 20-50 cm soil layer in the openwork forest belt - 141.4 mg/kg. The P2O5 content in the lower soil layer ranges from 62.1 mg/kg to 142.0 mg/kg. The highest P2O5 content of 168.0 mg/kg was found in the 0-20 cm soil layer of the openwork-blow forest belt. The amount of K2O in the upper soil layer is the same for all variants, in the lower layer it varies from 73.3 to 91.0 mg/kg. The openwork-blown forest belt has an available moisture reserve of 44.88 mm, the openwork belt - 51.03 mm, and the dense belt - 67.24 mm, which, compared to the dense forest belt, the moisture content of the openwork-blown belt is 33.27% lower and that of the openwork belt is 24.11% lower. Investigating the dynamics of changes in moisture productivity and moisture availability during the growing season (May-October) in all variants of the experiment both in 0-20 cm and 0-50 cm soil layer, a significant decrease in these indicators is observed. Thus, in the openwork-blown forest strip in the 0-20 cm soil layer in May, from 68 mm and 78%, these indicators decreased in October to 17 mm and 18%, respectively, i.e., in terms of moisture productivity by 4 times and moisture availability by 4.3 times. This indicates that the productivity and moisture content of the experimental forest strips are much higher compared to the control (open field), where the moisture productivity is 42 mm and the moisture availability is 35%. At a depth of up to 50 cm, the soil moisture content in the blown forest strips is one and a half to two times less than in the openwork forest strips. In favorable years, this difference in soil moisture remains throughout the growing season. The growth and development of tree species in the first half of the growing season (May-June) is carried out at the expense of moisture accumulated during snowmelt or winter rains in the lower soil layers. In the second half of the growing season, tree species receive moisture from summer precipitation that falls in late July and early August and accumulates in the upper soil layers.

A new oxidative pathway of nitric oxide production from oximes in plants

Nitric oxide (NO) is an essential reactive oxygen species and a signal molecule in plants. Although several studies have proposed the occurrence of oxidative NO production, only reductive routes for NO production, such as the nitrate (NO-3) -upper-reductase pathway, have been evidenced to date in land plants. However, plants grown axenically with ammonium as the sole source of nitrogen exhibit contents of nitrite and NO3−, evidencing the existence of a metabolic pathway for oxidative production of NO. We hypothesized that oximes, such as indole-3-acetaldoxime (IAOx), a precursor to indole-3-acetic acid, are intermediate oxidation products in NO synthesis. We detected the production of NO from IAOx and other oximes catalyzed by peroxidase (POD) enzyme using both 4-amino-5-methylamino-2′,7′-difluorescein fluorescence and chemiluminescence. Flavins stimulated the reaction, while superoxide dismutase inhibited it. Interestingly, mouse NO synthase can also use IAOx to produce NO at a lower rate than POD. We provided a full mechanism for POD-dependent NO production from IAOx consistent with the experimental data and supported by density functional theory calculations. We showed that the addition of IAOx to extracts from Medicago truncatula increased the in vitro production of NO, while in vivo supplementation of IAOx and other oximes increased the number of lateral roots, as shown for NO donors, and a more than 10-fold increase in IAOx dehydratase expression. Furthermore, we found that in vivo supplementation of IAOx increased NO production in Arabidopsis thaliana wild-type plants, while prx33-34 mutant plants, defective in POD33-34, had reduced production. Our data show that the release of NO by IAOx, as well as its auxinic effect, explain the superroot phenotype. Collectively, our study reveals that plants produce NO utilizing diverse molecules such as oximes, POD, and flavins, which are widely distributed in the plant kingdom, thus introducing a long-awaited oxidative pathway to NO production in plants. This knowledge has essential implications for understanding signaling in biological systems.

Effects of Different Manures in Combination with Fulvic Acid on the Abundance of N-Cycling Functional Genes in Greenhouse Soils

To investigate the effects of different manures in combination with fulvic acid on the abundance of N-cycling functional genes in greenhouse soils, Chinese cabbage was planted for three growing seasons. A total of six treatments—pig manure (P), pig manure + fulvic acid (PH), chicken manure (C), chicken manure + fulvic acid (CH), sheep manure (S), sheep manure + fulvic acid (SH) and no fertilization (CK)—were set up. The abundance of 13 soil N-cycling functional genes (gdhA, amoA-1, amoA-2, amoB, narG, nirK-1, nirK-2, nirK-3, nirS-1, nirS-2, nirS-3, nosZ and nifH) were investigated after the harvest of the third growing season using a gene chip approach. The results showed that fertilization treatments increased the abundance of most N-cycling functional genes in the soil, such as nitrification genes amoA-2 and amoB as well as denitrification genes narG, nirK-1, nirS-1 and nirS-2, with the stronger influence of sheep and pig manure than chicken manure. Fortunately, the additional fulvic acid reduced the increasing effect resulting from pig, chicken and sheep manure application. The abundance of functional genes for nitrogen cycling in soil was positively correlated with the content of soil organic matter, available phosphorus and NO3−-N, and negatively correlated with electrical conductivity. Overall, fertilization treatments increased soil nitrification and denitrification genes abundance, with a risk of increasing soil nitrogen loss, but the supplementary fulvic acid could limit the increase. In this study, it was concluded that the sheep manure (31.3 t/ha) + fulvic acid (7.5 kg/ha) treatment was more powerful in regulating the abundance of N-cycling functional genes in soil.

Synergistic effect and mechanism of nZVI/LDH composites adsorption coupled reduction of nitrate in micro-polluted water

In this study, nZVI/LDH composites were prepared by loading nano zero-valent iron (nZVI) on layered double hydroxide (LDH) surface for adsorption coupled reduction of nitrate (NO3--N). The results showed that the removal of NO3--N and total nitrogen (TN) by the nZVI/LDH composites was 88.64% and 77.63%, respectively, with a selectivity of 55.21% for N2 and only 1.86% for ammonia nitrogen (NH4+-N) within 180 min. The valence states of various N forms during the adsorption-reduction process were investigated. The mechanism of synergistic adsorption-reduction degradation of NO3--N was proposed by measuring the contents of NO3--N, nitrite (NO2--N), and NH4+-N in the aqueous and adsorbed phases in the reaction process, including rapid adsorption of initial NO3--N, chemical reduction of adsorbed NO3--N and resorption of the final product. The nZVI/LDH also maintained up to 82.56% NO3--N removal in natural water, with aging experiments proved that the composites maintained 60.48% NO3--N removal after 15 days. Therefore, the composites may have great application prospects for NO3--N removal in micro-polluted water.

Hydrogeochemical evaluation with emphasis on nitrate and fluoride in urban and rural drinking water resources in western Isfahan province, central Iran.

Nitrate (NO3-) and fluoride (F-) are two major potential contaminants found in the groundwater of Iran. These contaminants are highly dangerous to humans if consumed more than the safe limit prescribed by the WHO. Therefore, in this study, the urban and rural drinking water resources of Isfahan province (central Iran) were investigated to evaluate the quality of groundwater from the perspective of NO3- and F-. The calculated saturation index (SI) shows that the majority of samples are mainly undersaturated or in equilibrium with respect to potential minerals. The most likely interpretation for undersaturation with respect to most minerals is either that the minerals are not present if they are reactive or if they are present, then they are not reactive. This study reveals that the majority of the groundwater samples belong to the Ca-Mg-HCO3 water type. Further, in this study, potential physicochemical variables have been used to calculate entropy weighted water quality index (EWQI). The EWQI reveals that the majority of the groundwater in the area is of good quality. Results show that the water chemistry in the area is largely governed by the water-rock interaction. This study based on large data sets reveals that the majority of drinking water resources are uncontaminated by F-. However, the groundwater is found to be largely contaminated by NO3-. The bivariate plot suggests that the unscientific farming practices and overuse of manures and fertilizers are largely responsible for high content of NO3-. Therefore, emphasis should be given on the cost-effective environmentally friendly fertilizers. The findings from this study will aid the governing authorities and concerned stakeholders to understand the hydrogeochemical evolution of groundwater in this region. The results will help formulate policies in the area for sustainable water supply.

The Effects of Biochar-Based Organic Fertilizer and Mineral Fertilizer on Soil Quality, Beet Yield, and Sugar Yield

The addition of biochar-based organic fertilizer (BOF) can improve sugar beet yield, but its effects on the growth of sugar beet and on soil quality at different densities remain unclear. Six treatments, comprising two densities D1 and D2 (80,000 and 90,000 plant ha−1) and three application rates B1, B2, and B3 (2.75, 3.25, and 3.75 t ha−1) of BOFs + mineral fertilizer, respectively, are investigated in this research. The mineral fertilizers are typically used to supplement the total N, P2O5, and K2O deficiencies. The BOFs were used in the soil before sowing, and the mineral fertilizer was added to the soil after the first pair of true leaves was grown. At 160 days after sowing (DAS), the root-to-shoot ratio under the D2B2 treatment was significantly higher than that under the other treatments. The effect of density on the photosynthesis rate of sugar beet was not significant. The BOF application amount and density exerted interaction effects on soil physicochemical properties and the activities of different soil enzymes affecting each other. Both the D2B2 and D2B3 treatments reduced the content of NO3–-N in the 40–60 cm soil layer. Combined 90,000 plants ha–1 with 3.25 t ha−1 BOFs can increase the soil nutrient content of the 0–60 cm soil layer, improve the rhizosphere soil environment, promote the uniform distribution of dry matter, and increase sugar production.

Biodegradable mulch films improve yield of winter potatoes through effects on soil properties and nutrients

Biodegradable mulch films are recognized as a promising substitute of polyethylene (PE) films to alleviate the “white pollution”. Biodegradable mulch films with optimum degradation rates increase crop yield even compared to PE films. However, the mechanisms underlying this yield-increasing effect remains elusive. In this study, three biodegradable film treatments (BFM1, BFM2 and BFM3) and one PE film treatment (PFM) were used to evaluate their effects on soil and winter potatoes, and a partial least squares path model (PLS-PM) was constructed to investigate their relationships. The degradation rates of films under different treatments were ranked as BFM3 > BFM2 >BFM1 > PFM, and presented distinctive effects on soil properties and nutrients, structure of soil bacterial community, and yield traits of winter potatoes. The PLS-PM showed that mulch treatments affected potato yield through effects on soil properties (soil water and temperature) and soil nutrients (TOC, DOC, TN and NO3--N). The disintegration of the biodegradable films decreased soil water content and temperature, and reduced the loss of soil nutrients in the topsoil at the later growth stage of winter potatoes compared to PE films. Additionally, the elevated content of soil TN and NO3--N under treatment BFM1 may play a key role in its yield-increasing effect on potatoes compared to treatments PFM and BFM2. Thus, biodegradable mulch films with proper degradation rates regulate soil TN and NO3--N through their effects on soil water and temperature, and subsequently improve the yield of winter potatoes compared to PE mulch films.

Functional Characterisation of the Transcription Factor GsWRKY23 Gene from Glycine soja in Overexpressed Soybean Composite Plants and Arabidopsis under Salt Stress.

WRKY proteins are a superfamily of transcription factors (TFs) that play multiple roles in plants' growth, development, and environmental stress response. In this study, a novel WRKY gene called GsWRKY23 that is specifically upregulated in salt-tolerant Glycine soja accession BB52 seedlings was identified by transcriptomic analysis under salt stress. How the physiological functions and mechanisms of the GsWRKY23 gene affect salt tolerance was investigated using transformations of soybean hairy roots and Arabidopsis, including wild-type (WT) and atwrky23-mutant plants. The results showed that GsWRKY23 in the roots, stems, and leaves of BB52, along with its promoter in the cotyledons and root tips of GsWRKY23pro::GUS Arabidopsis seedlings, displayed enhanced induction under salt stress. GsWRKY23 localises to the nucleus and shows transcriptional activation ability in yeast cells. Compared to GsWRKY23-RNAi wild soybean hairy-root composite plants under salt stress, obvious improvements, such as superior growth appearance, plant height and fresh weight (FW), and leaf chlorophyll and relative water content (RWC), were displayed by GsWRKY23-overexpressing (OE) composite plants. Moreover, their relative electrolytic leakage (REL) values and malondialdehyde (MDA) contents in the roots and leaves declined significantly. Most of the contents of Na+ and Cl- in the roots, stems, and leaves of GsWRKY23-OE plants decreased significantly, while the content of K+ in the roots increased, and the content of NO3- displayed no obvious change. Ultimately, the Na+/K+ ratios of roots, stems, and leaves, along with the Cl-/NO3- ratios of roots and stems, decreased significantly. In the transgenic WT-GsWRKY23 and atwrky23-GsWRKY23 Arabidopsis seedlings, the salt-induced reduction in seed germination rate and seedling growth was markedly ameliorated; plant FW, leaf chlorophyll content, and RWC increased, and the REL value and MDA content in shoots decreased significantly. In addition, the accumulation of Na+ and Cl- decreased, and the K+ and NO3- levels increased markedly to maintain lower Na+/K+ and Cl-/NO3- ratios in the roots and shoots. Taken together, these results highlight the role of GsWRKY23 in regulating ionic homeostasis in NaCl-stressed overexpressed soybean composite plants and Arabidopsis seedlings to maintain lower Na+/K+ and Cl-/NO3- ratios in the roots and shoots, thus conferring improved salt tolerance.

Exposure to three herbicide mixtures influenced maize root-associated microbial community structure, function and the network complexity

Herbicide mixtures are a new and effective agricultural strategy for managing suppress weed resistance and have been widely used in controlling weeding growth in maize fields. However, the potential ecotoxicological impact of these mixtures on the microbial community structure and function within various root-associated niches, remains inadequately understood. Here, the effects of nicosulfuron, mesotrione and atrazine on soil enzyme activity and microbial community structure and function were investigated when applied alone and in combination. The findings indicated that herbicide mixtures exhibit a prolonged half-life compared to single herbicides. Ecological niches are the major factor influencing the structure and functions of the microbial community, with the rhizosphere exhibiting a more intensive response to herbicide stress. Herbicides significantly inhibited the activities of soil functional enzymes, including dehydrogenase, urease and sucrose in the short-term. Single herbicide did not drastically influence the alpha or beta diversity of the soil bacterial community, but herbicide mixtures significantly increased the richness of the fungal community. Meanwhile, the key functional microbial populations, such as Pseudomonas and Enterobacteriaceae, were significantly altered by herbicide stress. Both individual and combined use of the three herbicides reduced the complexity and stability of the bacterial network but increased the interspecific cooperations of fungal community in the rhizosphere. Moreover, by quantification of residual herbicide concentrations in the soil, we showed that the degradation period of the herbicide mixture was longer than that of single herbicides. Herbicide mixtures increased the contents of NO3−-N and NH4+-N in the soil in the short-term. Overall, our study provided a comprehensive insight into the response of maize root-associated microbial communities to herbicide mixtures and facilitated the assessment of the ecological risks posed by herbicide mixtures to the agricultural environment from an agricultural sustainability perspective.

The introduction of nitrogen from coal into the surface watershed nitrogen cycle due to coal mining activity

Human activity has doubled the turnover rate of the terrestrial nitrogen cycle, leading to a series of environmental problems. A little-studied nitrogen source in terrestrial and aquatic environments is the nitrogen release associated with rock strata. Southwest China features the largest continuous karsts in the world, featuring a fragile ecological environment but abundant coal resources. The current study selected a typical coal mining area to evaluate the migration and transformation of nitrogen related to coal mining in surface watershed. The findings reveal that the total nitrogen in coal seams was as high as 10,162.3 mg/kg, mainly in the form of organic nitrogen, followed by NH4+-N, while the content of NO3−-N was negligible. Based on the isotope fractionation and the co-evolution between Δ15NNO3-NH4 and δ15N-NO3−/δ15N-NH4+, coal mining changed the coal seams' oxidation-reduction state, resulting in the mineralization of organic nitrogen to NH4+-N. Next, NH4+-N gradually oxidized to NO3−-N. Various forms of coal-origin nitrogen may be leached out by acid mine drainage (AMD), potentially contributing >10 % of NO3−-N and 90 % of NH4+-N to the surface river. Another nitrogen source that requires serious consideration is the wide use of ammonium nitrate explosives in coal mining, as blasting residues may contribute about another 10 % to NO3−-N in surface water. Since organic nitrogen accounts for >90 % of extractable nitrogen, the release of coal-origin nitrogen may contribute much more to the total nitrogen in surface water than to NO3−-N. Based on the fractionation of nitrogen and oxygen isotopes of nitrate, low-pH AMD promotes the volatilization of nitrate in the form of nitric acid. The conversion of different forms of nitrogen in AMD will be the focus of future attention.