Contents Of Volume Research Articles

Evaluation of Select Monarda Taxa in Montane and Piedmont Regions of Georgia: II. Floral Morphology and Nectar Production

We analyzed the floral morphology and nectar production of several cultivars and species of Monarda representing five cultivars and four species grown in Georgia Piedmont and Montane regions. Over the course of two seasons, we detected significant differences among the samples in terms of inflorescence size, petal lobe and corolla widths and lengths, and total sugar content. M. didyma had larger glomerules, longer corollas and petal lobes, and higher nectar volume and total sugar content per flower. M. fistulosa and M. punctata had smaller glomerules, corolla and petal lobe lengths, and total sugar content per flower. Petal lobe and corolla length strongly correlated with sucrose and nectar production. Combined with data on horticultural performance, these results could be valuable in informing breeding goals for conservation-oriented landscape plants.

Behavior of steel fiber-reinforced coal gangue concrete beams under impact load

In order to promote the use of new sustainable steel fibers (SF) reinforced coal gangue aggregates (CGA) concrete, impact tests were performed in this paper. The impact resistance of steel fiber-reinforced coal gangue concrete was investigated using a self-developed drop-weight facility. The test variables were the CGA replacement rate (0 %, 50 %, and 100 %), the SF volume content (0 %, 0.75 %, and 1.5 %), and the drop height of the drop-weight (0.5 m and 1.0 m). Using 15 kg of hard steel as a drop-weight, the drop-weight impact test was performed on steel fiber-reinforced coal gangue concrete beams with a span of 300 mm; the damage pattern, impact reaction force-time, displacement-time relationships, and energy absorbed by steel fiber-reinforced coal gangue concrete beams were studied. The test shows that, compared with natural aggregate, CGA reduces concrete's static mechanical properties and impact resistance, and adding SF improves the static mechanical properties and impact resistance of coal gangue concrete. As the CGA replacement rate increased from 0 % to 50 % and 100 %, the reaction force of impact of the specimens decreased by 2.54 %− 6.06 % and 3.69 %− 12.33 %. The SF volume content was increased from 0 % to 0.75 % and 1.5 %, and the reaction force of impact of the specimens was increased by 23.05 %− 32.34 % and 72.27 %− 81.84 %, respectively. The impact resistance of steel fiber-reinforced concrete beams under impact loading was investigated numerically using a nonlinear explicit finite element model in LS-DYNA. The Finite Element results were validated with experimental results. Based on the validated finite element model, a parametric study was carried out to investigate the effects of coal gangue replacement rate, steel fiber volume content, and drop height of the drop weight on the behavior of the beams. This study might provide a basis for the future engineering project applications of steel fiber-reinforced coal gangue concrete under impact conditions.

Study of the Performance of Emulsified Asphalt Shotcrete in High-Altitude Permafrost Regions

To improve the performance of shotcrete in high-altitude and low-temperature environments, emulsified asphalt shotcrete (EASC), which can be used in negative-temperature environments, was prepared by using low-freezing-point emulsified asphalt, calcium aluminate cement, and sodium pyrophosphate as modified materials. The effect of emulsified asphalt on the performance of shotcrete was investigated through concrete spraying and indoor tests. Then, the modification mechanism of emulsified asphalt with respect to EASC was analyzed by combining scanning electron microscopy images and the pore structure characteristics of EASC. The results showed that in a negative-temperature environment, the incorporation of emulsified asphalt delayed the formation of the peak of the cement hydration exotherm, slowed the rate of the cement hydration exotherm, reduced the thermal perturbation of permafrost by EASC, increased the cohesion of the concrete, improved the bond strength between EASC and permafrost, and reduced the rate of rebound. The mechanical strength of the studied EASC decreased upon increasing the amount of emulsified asphalt in the admixture, and its resistance to cracking gradually improved. A content of less than 5% emulsified asphalt could improve the internal pore structure of EASC, thus improving its durability. Increasing the content of emulsified asphalt affected the hydration process of the cement, and the volume content of the capillary pores and macropores increased, which reduced the durability of the EASC.

Evaluating Sex-specific Differences in Abdominal Fat Volume and Proton Density Fat Fraction on MRI Scans Using Automated nnU-Net-based Segmentation.

"Just Accepted" papers have undergone full peer review and have been accepted for publication in Radiology: Artificial Intelligence. This article will undergo copyediting, layout, and proof review before it is published in its final version. Please note that during production of the final copyedited article, errors may be discovered which could affect the content. Sex-specific abdominal organ volume and proton density fat fraction (PDFF) in people with obesity during a weight loss intervention was assessed using automated multiorgan segmentation of quantitative water-fat MRI. An nnU-Net architecture was employed for automatic segmentation of abdominal organs, including visceral (VAT) and subcutaneous adipose tissue (SAT), liver, psoas and erector spinae muscle, based on quantitative chemical shiftencoded MRI and using ground truth labels generated from participants of the Lifestyle Intervention (LION) study. Each organ's volume and fat content were examined in 127 participants (73 female, 54 male; body mass index, 30-39.9 kg/m2) and in 81 participants (54 female, 32 male) of these after an 8-week formula-based low-calorie diet. Dice scores ranging from 0.91 to 0.97 were achieved for the automatic segmentation. PDFF was found to be lower in VAT compared with SAT in both male and female participants. Before intervention, females exhibited higher PDFF in SAT (90.6% versus 89.7%, P < .001) and lower PDFF in liver (8.6% versus 13.3%, P < .001) and VAT (76.4% versus 81.3%, P < .001) compared with males. This relation persists after intervention. As a response to caloric restriction, male participants lost significantly more VAT volume (1.76 L versus 0.91 L, P < .001) and showed a higher decrease in SAT PDFF (2.7% versus 1.5%, P < .001) than female participants. Automated body composition analysis on quantitative water-fat MRI data provides new insights for understanding sex-specific metabolic response to caloric restriction and weight loss in people with obesity. Published under a CC BY 4.0 license.

Effects of amount and geometrical properties of steel fiber on shear behavior of high-strength concrete beams without shear reinforcement

This study aims to investigate the effects of the geometrical properties and volume content of steel fibers on the shear resistance of reinforced high-strength concrete (HSC) beams without stirrups. Hooked-end and twisted steel fibers with different aspect ratios ranging from 60 to 100 were used at volume fractions of 0.5%–1.0%. The shear strength of HSC beams without stirrups significantly increased due to the addition of steel fibers and by increasing their volume fraction by up to 1.0%, resulting in approximately 1.7 times higher shear resistance. A higher fiber aspect ratio was effective in improving the resistance to shear failure of HSC beams without stirrups and in enhancing beam deformability relative to the increase in shear strength. Using twisted steel fibers led to better shear resistance than hooked-end steel fibers, resulting in 1.11- and 3.34-times higher shear strength and ductility at the same fiber volume fraction on average. The shear strength of steel fiber-reinforced concrete (SFRC) beams was also predicted using eight different models. Narayanan's model most precisely predicted the shear strengths of reinforced SFRC beams without shear reinforcement, with an average vu,exp/vu,pred ratio of 1.16. The shear strength increased with the decrease in shear span-to-depth (a/d) ratio and the increase in the fiber factor. The contribution of steel fibers to shear strength was greater for deep beams than slender beams. The predicted shear strengths of deep beams and high-strength SFRC showed a significant deviation from the actual value and required careful consideration.

The GWAS SNP rs80207740 modulates erythrocyte traits via allele-specific binding of IKZF1 and targeting XPO7 gene.

Genome-wide association studies have identified many single nucleotide polymorphisms (SNPs) associated with erythrocyte traits. However, the functional variants and their working mechanisms remain largely unknown. Here, we reported that the SNP of rs80207740, which was associated with red blood cell (RBC) volume and hemoglobin content across populations, conferred enhancer activity to XPO7 gene via allele-differentially binding to Ikaros family zinc finger 1 (IKZF1). We showed that the region around rs80207740 was an erythroid-specific enhancer using reporter assays, and that the G-allele further enhanced activity. 3D genome evidence showed that the enhancer interacted with the XPO7 promoter, and eQTL analysis suggested that the G-allele upregulated expression of XPO7. We further showed that the rs80207740-G allele facilitated the binding of transcription factor IKZF1 in EMSA and ChIP analyses. Knockdown of IKZF1 and GATA1 resulted in decreased expression of Xpo7 in both human and mouse erythroid cells. Finally, we constructed Xpo7 knockout mouse by CRISPR/Cas9 and observed anemic phenotype with reduced volume and hemoglobin content of RBC, consistent to the effect of rs80207740 on erythrocyte traits. Overall, our study demonstrated that rs80207740 modulated erythroid indices by regulating IKZF1 binding and Xpo7 expression.

Natural bio-waste-derived 3D N/O self-doped heteroatom honeycomb-like porous carbon with tuned huge surface area for high-performance supercapacitor

Supercapacitor electrodes (SCs) of carbon-based materials with flexible structures and morphologies have demonstrated excellent electrical conductivity and chemical stability. Herein, a clean and cost-effective method for producing a 3D self-doped honeycomb-like carbonaceous material with KOH activation from bio-waste oyster shells (BWOSs) is described. A remarkable performance was achieved by the excellent hierarchical structured carbon (HSC-750), which has a large surface area and a reasonably high packing density. The enhanced BWOSs-derived HSC-750 shows an ultrahigh specific capacitance of 525 F/g at 0.5 A g−1 in 3 M KOH electrolyte, as well as high specific surface area (2377 m2 g−1), pore volume (1.35 cm3 g−1), nitrogen (4.70%), and oxygen (10.58%) doping contents. The SCs also exhibit exceptional cyclic stability, maintaining 98.5% of their capacitance after 10,000 charge/discharge cycles. The two-electrode approach provides a super high energy density of 28 Wh kg−1 at a power density of 250 W kg−1 in an alkaline solution, with remarkable cyclability after 10,000 cycles. The study demonstrates the innovative HSC synthesis from BWOSs precursor and cost-effective fabrication of 3D N/O self-doped heteroatom HSC for flexible energy storage.

Stress-Strain State of Steel Fiber-Reinforced Concrete under Compression Taking into Account Unloading from Inelastic Region

The purpose of the study is to examine the physical and mechanical characteristics of steel fiber-reinforced concrete under compression, including: modulus of elasticity, Poisson ratio, values of ultimate strains under compression, values of compressive strength with different percentages of dispersed reinforcement. An experimental investigation program, which included the production of cube samples measuring 100×100×100 mm, as well as a compression test under static loading, taking into account unloading from the region of inelastic deformations, was developed and carried out. Two types of steel fiber were chosen as dispersed reinforcement: hooked end and wave shape. The volume content of steel fiber in the cube samples was 0.5, 1.0, 1.5 and 2.0 %. As a result of the investigation, the strength and deformation characteristics of steel fiber reinforced concrete under compression were obtained. Based on the experimental data, actual strain diagrams of steel fiber reinforced concrete were constructed, taking into account the type of reinforcing fibers and the percentage of reinforcing fiber. Based on the obtained diagrams, a law of deformation of steel fiber reinforced concrete is proposed, which can be described by a polynomial function of the fourth order with constant coefficients that determine the shape of the stress-strain curve. The presented research results can be used in developing a methodology for physically nonlinear analysis of steel fiber reinforced concrete elements with a percentage of dispersed reinforcement from 0.5 to 2.0 %.

Effect of Nano-TiO2 and Polypropylene Fiber on Mechanical Properties and Durability of Recycled Aggregate Concrete

In order to promote the engineering application of recycled concrete, the effects of PPF and nano-TiO2 dioxide on the mechanical properties and durability of recycled concrete were studied.Polypropylene fiber recycled concrete(PRAC) and nano-TiO2 recycled concrete(TRAC) were prepared by adding different volume contents of PPF and nano-TiO2. The experimental findings demonstrated that the PPF and nano-TiO2 improved the splitting tensile strength of RAC better than the compressive strength. When the volume content of nano-TiO2. and PPF is 0.8% and 1.0%, respectively, the corresponding splitting tensile strength of concrete reaches the maximum value(3.4 and 3.7 MPa). The contribution rates of nano-TiO2 and PPF with different volume contents to the mechanical properties of RAC have optimal values, which are 0.4 and 1.0%, respectively. The incorporation of nano-TiO2 and PPF can effectively inhibit the loss of RAC mass and the generation of pores under freeze–thaw conditions, and slow down the decrease of dynamic elastic modulus. When the volume content of PPF is 1.0% and the volume content of nano-TiO2 is 0.4%, the protection effect on the internal structure of RAC is better, and its carbon resistance is better. The results of RSM model analysis and prediction show that both PPF and nano-TiO2 can be used as admixture materials to improve the mechanical properties and durability of RAC, and the comprehensive improvement effect of PPF on RAC performance is better than that of nano-TiO2.

Mechanical and durability performance of self-compacting mortars made with different industrial by-products as fillers

This study evaluated the performance over time of Self-Compacting Mortars (SC mortars) with different industrial by-products simultaneously; Electric Arc Furnace Dust (EAFD) and fly ash, conforming (FA) or not conforming (NcFA), from coal-fired power plant or recovery filler from hot-mix asphalt plant (RF). Different SC mortars mixes were produced; three mixes with different EAFD ratio incorporation (0 %, 10 %and 20 %) for each the other industrial by-products (FA, NcFA and RF) substituted of cement at 30 %; and additional three mixes with 60 % of cement replacement by FA and the same EAFD incorporation ratios. To study the performance over time of these SC mortars, tests such as electrical resistivity, ultrasonic pulse velocity, drying shrinkage, capillarity, compressive and flexural strength were carried out up to 91 days of curing. The SC mortar compositions was evaluated by TGA/DTA and DRX analysis. Previously, the by-products used were characterized. The EAFD incorporation showed a strong influence in SC mortars, independently of the industrial by-product used. The initial inhibition of development of hardening in SC mortars due to the presence of ZnO in EAFD was lifted over time up to some extent. The simultaneous use of different industrial-by products in SC mortars in high volume content walks towards the sustainability in the construction sector.

Experimental Investigation on Shear Behavior of Non-Stirrup UHPC Beams under Larger Shear Span–Depth Ratios

Due to the extraordinary mechanical properties of ultra-high-performance concrete (UHPC), the shear stirrups in UHPC beams could potentially be eliminated. This study aimed to determine the effect of beam height and steel fiber volume content on the shear behavior of non-stirrup UHPC beams under a larger shear span–depth ratio (up to 2.8). Eight beams were designed and fabricated including six non-stirrup UHPC beams and two comparing stirrup-reinforced normal concrete (NC) beams. The experimental results demonstrated that the steel fiber volume content could be a crucial factor affecting the ductility, cracking strength, and shear capacity of non-stirrup UHPC beams and altering their failure modes. Additionally, the height of the beam had a considerable effect on its shear resistance. French standard formulae were more accurate for the UHPC beams with larger shear span–depth ratios, PCI-2021 formulae greatly overestimated the shear capacity of UHPC beams with larger shear span–depth ratios, and Xu’s formulae were more accurate for the steel fiber-reinforced UHPC beams with larger shear span–depth ratios. In summary, French standard formulae were the most suitable formulae for predicting the shear capacity of UHPC beams in this paper.

Minimum Shear Reinforcement for Reactive Powder Concrete Beams

The aim of this research was to determine the minimal requirements for shear reinforcement for reactive powder concrete (RPC) rectangular cross-sectional beams with a compressive strength of 157 MPa and a steel fiber volume content of 2.0% that remained constant for all the tested beams. Additionally, the recommendations of KCI-2012 and AFGC-2013 for the design of RPC beams as well as the shear design requirements of ACI 314-2014 when applied to RPC beams were studied. Utilizing a three-dimensional finite element program, a computational model was designed for forecasting the deformations and shear strength of the examined RPC beams. Both the shear-span-to-depth relationship (a/d) and the minimal reinforcement web ratio, represented by the distance between stirrups and the diameter of the stirrup bars, are the key study parameters in this regard. According to this study’s experimental findings, increasing the given reinforcement of the web ratio has little influence on both the ultimate shear strength as well as the diagonal cracking strength of the beams. Additionally, the findings demonstrated that the ACI 318-2014 maximum stirrup spacing requirement of 0.5 d can safely be extended to 0.75 d for beams that are relatively short. Compared to what ACI 318-2014 mandates, the suggestions of AFGC-2013 and KCI-2012 are more cautious and safe. According to the AFGC-2013 criteria, the mean proportion of Vfb to projected Vu,AFGC is roughly 58.3%, whereas the mean proportion of vs. and Vc is just 41.7%. The deformation response and ultimate shear strength of the examined RPC beams were well predicted by the designed model using finite elements when metal fibers were taken into account.

Nanofabrication, characterisation and modelling of soft-in-hard FeCo–FePt magnetic nanocomposites

Advanced nanofabrication exploiting e-beam lithography has been used to prepare nanocomposites consisting of periodic arrays of soft magnetic FeCo-based nano-inclusions of variable dimensions, embedded in a hard magnetic FePt matrix. Nanocomposites with non-magnetic (Pt) inclusions and single phase hard magnetic FePt microstructures were prepared as reference samples. The formation of Kirkendall voids in the soft-in-hard nanocomposites, because of annealing-induced diffusion, has been identified through high resolution imaging and chemical analysis. Replication of the μm-scaled nanocomposite and hard magnetic units over mm-scaled surfaces allowed global magnetic characterisation of magnetisation reversal using standard magnetometry. Magnetic force microscopy imaging was used for spatially resolved magnetic characterisation in different remanent magnetic states. Both of these experimental techniques were used to study the influence of the size, volume content and nature of the nano-inclusions on magnetisation reversal. The well-defined geometry and nano-scaled size of the inclusions render these nanocomposites as model systems for micromagnetic simulations and very good agreement was achieved between measured and simulated magnetisation reversal curves. This accordance motivates future in-silico optimisation of such soft-in-hard nanocomposites, which may serve as model systems to guide the design of new high performance bulk magnets which are less dependent on critical elements.

Production of γ–aminobutyric acid–enriched sourdough bread using an isolated Pediococcus pentosaceus strain JC30

A γ–aminobutyric acid (GABA)–producing strain JC30 was isolated from traditional kimchi, which was identified as Pediococcus pentosaceus by 16S rDNA sequencing. P. pentosaceus JC30 was highly tolerant to acid, bile salt, and high temperatures. The survival rate of JC30 in MRS medium (pH 2.5) for 3 h was 60.96 %. Furthermore, the survival rate of JC30 in MRS medium with 3 mg/mL bile salt for 24 h was 86.62 %. The survival rate of JC30 in MRS medium at 56 °C and 58 °C for 10 min was 97.17 % and 78.20 %, respectively. When 2 % v/v JC30 (8.0 log10 CFU/mL) was added to prepare sourdough and the sourdough was then used to make bread, the bread had a higher specific volume (5.13 ± 0.12 mL/g) and GABA content (3.32 ± 0.04 mg/g DW) than the control.

Effect of Maize (Zea mays) Substitution by Sprouted Off-season Sorghum (Sorghum bicolor L. Moench) on the Growth and Physiological Response of Broiler Chickens (Gallus gallus) in Maroua, Far North, Cameroon

Effect of Maize (Zea mays) Substitution by Sprouted Off-season Sorghum (Sorghum bicolor L. Moench) on the Growth and Physiological Response of Broiler Chickens (Gallus gallus) in Maroua, Far North, Cameroon

Contents of Volume 129

Contents of Volume 129

Comparison of quality, dietary fiber and glycemic index of whole wheat breads using various modern and old wheats and investigate the effect of xylanase

Six bread wheat varieties (modern wheats) widely grown in Turkey and nine different bread wheat varieties registered prior to 1970 (old wheats) were used in the present study. This study aimed to determine and compare the quality characteristics, dietary fiber contents and in vitro glycemic index of whole wheat breads produced using modern and old bread wheat varieties. The effect of xylanase on the quality, staling and dietary fiber content of whole wheat breads was also investigated. Mean sedimentation and modified sedimentation values, Farinograph development time, stability, quality number of whole wheat flours produced by modern wheats were higher than those of the old varieties. Whole wheat breads of modern varieties had higher mean volume and lower firmness values as compared to old varieties. Whole wheat breads of old wheat varieties had higher mean soluble dietary fiber content as compared to that of modern wheat varieties. Xylanase addition generally had an increasing effect on bread volume and soluble dietary fiber contents and a softening effect on firmness values. There were no significant differences between the mean (in vitro) glycemic index values of whole wheat breads produced from modern and old varieties. The results in the present study indicated that modern bread wheat varieties had better whole wheat bread making quality as compared with old bread wheat varieties. In addition, xylanase usage in whole wheat bread formula had an improving effect on whole wheat bread quality.

Fly ash-GGBS blended geopolymer mortar for early engineering characteristic at ambient temperature

Geopolymer concept is by using industrial waste materials which is rich in silica and alumina, then activating them with alkaline solution. An appropriate binder combination that achieves high early age strength at ambient temperature using FA is still subject required. Thus, this study has been designed in two stages which involving the paste and mortar studies. The alkaline activator as a blend of sodium hydroxide and sodium silicate solution with SS/SH ratio of 2.5, NaOH molarity of 12 M and L/B ratio of 0.43 are used for geopolymer composite. Compressive strength, water absorption and shrinkage are tested on geopolymer mortar with various level of blended combination. High volume content (60–80 %) gave a promising early age compressive strength at ambient temperature. Metakaolin gave a further strength improvement on geopolymer mortar. Overall, the finding suggested that the geopolymer with high FA content is suitable for cement-based material repair product.

Cortical parenchyma wall width regulates root metabolic cost and maize performance under suboptimal water availability.

We describe how increased root cortical parenchyma wall width (CPW) can improve tolerance to drought stress in maize by reducing the metabolic costs of soil exploration. Significant variation (1.0 to 5.0 µm) for CPW was observed in maize germplasm. The functional-structural model RootSlice predicts that increasing CPW from 2 to 4 µm is associated with ca. 15% reduction in root cortical cytoplasmic volume, respiration rate, and nitrogen content. Analysis of genotypes with contrasting CPW grown with and without water stress in the field confirms that increased CPW is correlated with ca. 32 to 42% decrease in root respiration. Under water stress in the field, increased CPW is correlated with 125% increased stomatal conductance, 325% increased leaf CO2 assimilation rate, 73 to 78% increased shoot biomass, and 92 to 108% increased yield. CPW was correlated with leaf mesophyll midrib parenchyma wall width, indicating pleiotropy. GWAS analysis identified candidate genes underlying CPW. OpenSimRoot modeling predicts that a reduction in root respiration due to increased CPW would also benefit maize growth under suboptimal nitrogen, which requires empirical testing. We propose CPW as a new phene that has utility under edaphic stress meriting further investigation.

Short-term effects of obesity surgery versus low-energy diet on body composition and tissue-specific glucose uptake: a randomised clinical study using whole-body integrated 18F-FDG-PET/MRI

Aims/hypothesisObesity surgery (OS) and diet-induced weight loss rapidly improve insulin resistance. We aim to investigate the impact of either Roux-en-Y gastric bypass (RYGB) or sleeve gastrectomy (SG) surgery compared with a diet low in energy (low-calorie diet; LCD) on body composition, glucose control and insulin sensitivity, assessed both at the global and tissue-specific level in individuals with obesity but not diabetes.MethodsIn this parallel group randomised controlled trial, patients on a waiting list for OS were randomised (no blinding, sealed envelopes) to either undergo surgery directly or undergo an LCD before surgery. At baseline and 4 weeks after surgery (n=15, 11 RYGB and 4 SG) or 4 weeks after the start of LCD (n=9), investigations were carried out, including an OGTT and hyperinsulinaemic–euglycaemic clamps during which concomitant simultaneous whole-body [18F]fluorodeoxyglucose-positron emission tomography (PET)/MRI was performed. The primary outcome was HOMA-IR change.ResultsOne month after bariatric surgery and initiation of LCD, both treatments induced similar reductions in body weight (mean ± SD: −7.7±1.4 kg and −7.4±2.2 kg, respectively), adipose tissue volume (7%) and liver fat content (2% units). HOMA-IR, a main endpoint, was significantly reduced following OS (−26.3% [95% CI −49.5, −3.0], p=0.009) and non-significantly following LCD (−20.9% [95% CI −58.2, 16.5). For both groups, there were similar reductions in triglycerides and LDL-cholesterol. Fasting plasma glucose and insulin were also significantly reduced only following OS. There was an increase in glucose AUC in response to an OGTT in the OS group (by 20%) but not in the LCD group. During hyperinsulinaemia, only the OS group showed a significantly increased PET-derived glucose uptake rate in skeletal muscle but a reduced uptake in the heart and abdominal adipose tissue. Both liver and brain glucose uptake rates were unchanged after surgery or LCD. Whole-body glucose disposal and endogenous glucose production were not significantly affected.Conclusions/interpretationThe short-term metabolic effects seen 4 weeks after OS are not explained by loss of body fat alone. Thus OS, but not LCD, led to reductions in fasting plasma glucose and insulin resistance as well as to distinct changes in insulin-stimulated glucose fluxes to different tissues. Such effects may contribute to the prevention or reversal of type 2 diabetes following OS. Moreover, the full effects on whole-body insulin resistance and plasma glucose require a longer time than 4 weeks.Trial registrationClinicalTrials.gov NCT02988011FundingThis work was supported by AstraZeneca R&D, the Swedish Diabetes Foundation, the European Union’s Horizon Europe Research project PAS GRAS, the European Commission via the Marie Sklodowska Curie Innovative Training Network TREATMENT, EXODIAB, the Family Ernfors Foundation, the P.O. Zetterling Foundation, Novo Nordisk Foundation, the Agnes and Mac Rudberg Foundation and the Uppsala University Hospital ALF grantsGraphical