Tryptophan Content Research Articles

Some Nutritional Value Aspects of Barley and Oat and Their Impact in Human Nutrition and Healthy Life.

Nowadays, there is a general concern regarding the increasing global talk about functional foods that respond to our demands and needs as consumers in order to maintain health and body weight through a correctly balanced diet. Cereals are key elements of nutrition and a healthy diet, and they also play a significant role in health promotion due to the useful nutrient content. Therefore, this work aims to identify barley and oat genotypes suitable for human nutrition and to achieve practical results for their widespread use in preventing or treating certain chronic diseases by analyzing the nutritional and physical properties of 52 genotypes of oat and barley conserved in Suceava Gene Bank, Romania. The first part of this manuscript is the presentation of these accessions and the evaluation of their most important properties. For oat and barley cultivars, detailed processing was carried out, involving the computation of variation amplitude, coefficients of correlation and cluster analyses, both for biochemical (protein, lysine and tryptophan contents) and physical (test weight and seed weight) properties. The results indicated high variability between oat and barley varieties. Thus, according to the results, the 26 varieties of oat exhibited almost double the content of lysine compared to barley seeds, while tryptophan had higher values in barley than in oat seeds. Overall, both species play an essential role in human nutrition, barley being important because of its high protein content and higher productivity compared to oats, which, although not as productive, have better quality seeds due to their higher lysine content. The results presented are not only of scientific interest but also have practical implications for agriculture, food safety, nutrition and human health. The documented information will facilitate new studies needed to contribute to improving human nutrition and health.

Integrated transcriptomics, metabolomics and physiological analyses reveal differential response mechanisms of wheat to cadmium and/or salinity stress.

Many soils face dual challenges of cadmium (Cd) contamination and salinization. However, the response of crops, especially wheat, to combined Cd and salinity stress is not understood. Here, wheat was grown in a hydroponic model for 14 days under single and combined Cd and NaCl stresses. Growth parameters, tissue Cd2+ and Na+ contents, and leaf chlorophyll (Chl), O2•-, and MDA levels were determined. Comparative transcriptomic and metabolomic analyses of the leaves were performed. The results showed that combined stress had a greater inhibitory effect on Chl contents and generated more O2•- and MDA, resulting in more severe wheat growth retardation than those under Cd or NaCl stress. Stress-induced decrease in Chl levels may be attributed to the inhibition of Chl biosynthesis, activation of Chl degradation, or a decline in glutamate content. Cd addition weakened the promotional effect of NaCl on SOS1 gene expression, thereby increasing the Na+ content. Contrastingly, NaCl supplementation downregulated the Nramp and ZIP gene expressions related to Cd uptake and transport, thereby impeding Cd2+ accumulation. All stresses enhanced tryptophan content via promoting tryptophan biosynthesis. Meanwhile, Cd and NaCl stresses activated phenylpropanoid biosynthesis and purine metabolism, respectively, thereby increasing the levels of caffeic acid, fumaric acid, and uric acid. Activating the TCA cycle was important in the wheat's response to combined stress. Additionally, NaCl and combined stresses affected starch and sucrose metabolism, resulting in sucrose and trehalose accumulation. Our findings provide a comprehensive understanding of the response of wheat to the combined Cd and salinity stress.

Assessment of the Biological Activity of a Probiotic Fermented Milk Product with the Addition of Lactobacillus helveticus Cell-Free Supernatant

Products enriched with probiotic microorganisms have proven to possess immunomodulatory, antioxidant, hypo-cholesterolemic, hypotensive, and antimicrobial properties. Biologically active substances, which are by-products of microbial fermentation, have potential applications in various industries. Cell-free supernatants, depending on the microorganisms used and production conditions, can exhibit antimicrobial, antioxidant, bifidogenic, and other biological activities. This paper presents a study on the biological activity of a probiotic fermented milk product, supplemented with 0.01% lyophilized cell-free supernatant from Lactobacillus helveticus. The fermented milk product was developed based on a composition of Lacticaseibacillus rhamnosus F, Lactococcus cremoris CR201, and Propionibacterium shermanii E2. The research evaluated antimicrobial activity, Bifidobacteria growth stimulation, and the content of organic acids, amino acids, and B vitamins. It was found that adding lyophilized cell-free supernatant to the fermented milk product enhanced its biological activity. In particular, the experimental samples showed a threefold increase in vitamin B6 content compared to the control, reaching 22.412 μg/100 g. Additionally, the amino acid content in the experimental samples exhibited a significant increase of more than 100% in the essential amino acid tryptophan compared to the control. Notably, antimicrobial activity increased against several opportunistic strains. The experimental samples also showed a significant increase in lactic and formic acids, which may enhance the product’s inhibitory properties against pathogens. An increase in antioxidant activity was observed, potentially due to the higher content of tryptophan and vitamin B6. The positive effect of adding cell-free supernatant on the growth of Bifidobacteria was also demonstrated. Thus, the findings suggest that this cell-free supernatant can be recommended as an additive in the production of fermented milk products, food additives, dietary supplements, and animal feed.

Immune cells and tryptophan metabolism in the joint capsule tissue in rheumatoid arthritis

To the present day, many links in the pathogenesis of rheumatoid arthritis remain unclear, which leads to unsatisfactory results in its therapy.The aim. To study the cells involved in immune reactions and tryptophan metabolites in the joint capsule in rheumatoid arthritis.Materials and methods. The experiments were carried out on 40 Wistar rats. Rheumatoid arthritis was induced by intraperitoneal injection of a solution of type 2 collagen (Chondrex Inc., USA) in incomplete Freund’s adjuvant. On the days 7, 14 and 21, the content of tryptophan, kynurenine, 3-hydrokenurinine, L-5-hydrotryptophan in the joint capsule was determined using high-performance liquid chromatography. Cells with CD3, CD20 and CD68 in joint tissues were studied at the same time using the streptavidin-biotin-peroxidase method. We used enzyme-linked immunosorbent method to determine antibodies to citrulline-containing peptide. Statistical analysis was performed using the Jamovi, version 2.3 software.Results. The content of cells carrying CD3, CD20 and CD68 markers in the joint was high in experimental rheumatoid arthritis. In joint tissues, the content of tryptophan metabolites along the kynurenine pathway also increases and the concentration of metabolites along the serotonin pathway decreases. Direct positive correlations of cells carrying CD3, CD20 and CD68 differential clusters with the content of tryptophan metabolites along the kynurenine pathway and negative correlations with metabolites of the serotonin pathway were established.Conclusions. Cells carrying CD3, CD20 and CD68 markers and tryptophan metabolites – kynurenine and L-5-hydrotryptophan – play an important role in the pathogenesis of rheumatoid arthritis.

Tryptophan-rich diet and its effects on Htr7+ Tregs in alleviating neuroinflammation and cognitive impairment induced by lipopolysaccharide

BackgroundNeuroinflammation is a vital pathogenic mechanism for neurodegenerative diseases such as Alzheimer’s, schizophrenia, and age-related cognitive decline. Regulatory T cells (Tregs) exhibit potent anti-inflammatory properties and can modulate neurodegenerative diseases arising from central nervous system inflammatory responses. However, the role of Tregs in neuroinflammation-related cognitive dysfunction remains unclear. It is highly plausible that Htr7+ Tregs expressing unique genes associated with the nervous system, including the Htr7 gene encoding the serotonin receptor 5-HT7, play a pivotal role.MethodsMice were given a tryptophan-rich diet (with a tryptophan content of 0.6%) or a normal diet (with a tryptophan content of 0.16%). The neuroinflammation-mediated cognitive dysfunction model was established by intracerebroventricular injection of lipopolysaccharide (LPS) in 8-week-old C57BL/6J mice. The activation and infiltration of Tregs were measured using flow cytometry. Primary Tregs were cocultured separately with primary CD8+ T cells and primary microglia for in vitro validation of the impact of 5-HT and 5-HT7 receptor on Tregs. Prior to their transfer into recombination activating gene 1 (Rag1−/−) mice, Tregs were ex vivo transfected with lentivirus to knock down the expression of Htr7.ResultsIn this study, the tryptophan-rich diet was found to reverse LPS-induced cognitive impairment and reduce the levels of 5-HT in peripheral blood. The tryptophan-rich diet led to increased levels of 5-HT in peripheral blood, which in turn promoted the proliferation and activation of Htr7+ Tregs. Additionally, the tryptophan-rich diet was also shown to attenuate LPS-mediated neuroinflammation by activating Htr7+ Tregs. Furthermore, 5-HT and 5-HT7 receptor were found to enhance the immunosuppressive effect of Tregs on CD8+ T cells and microglia. In Rag1−/− mice, Htr7+ Tregs were shown to alleviate LPS-induced neuroinflammation and cognitive impairment.ConclusionsOur research revealed the ability of Htr7+ Tregs to mitigate neuroinflammation and prevent neuronal damage by suppressing the infiltration of CD8+ T cells into the brain and excessive activation of microglia, thereby ameliorating LPS-induced cognitive impairment. These insights may offer novel therapeutic targets involving Tregs for neuroinflammation and cognitive impairment.

The Effect of Herbicides and Biostimulant Application on the Seed Yield and Seed Quality of Soybean (Glycine max (L.) Merr.)

The profitability of growing soybean (Glycine max (L.) Merr.) is largely determined by the successful elimination of weeds and the weather conditions, the adverse effect of which can be minimized by the use of biostimulants. This study aims to evaluate the effect of several herbicides and biostimulants on the seed yield and contents of protein, fat, amino acids, and fatty acids in soybean seeds. The results demonstrate that the yield and quality of soybean seeds were most beneficially affected by the use of prosulfocarb immediately after sowing in combination with a subsequent foliar application of bentazone and imazamox with an adjuvant containing methyl oleate and fatty alcohol. This treatment ensured the highest seed yield (3.32 t ha−1), the highest contents in seeds of protein (342.4 g kg−1 DM), phenylalanine (15.65 g kg−1 DM), leucine (23.54 g kg−1 DM), and most of the endogenous amino acids. All herbicide treatments increased the contents of tryptophan, serine, and glutamic acid compared to the seeds without herbicide treatment. The study results indicate that soybean responded positively to all biostimulants, as indicated by a higher seed yield (from 5.3% to 11.3%), plant height, and contents of tryptophan, serine, glutamic acid, cysteine, tyrosine, and C18:1n9c + C18:1n9t acids in the seeds.

Strategic genetic amelioration of quality protein maize (QPM) germplasm and its utilization in hybrid breeding

Abstract Use of diverse germplasm for generating heterotic hybrids is the foremost requirement in maize. The present study was conducted by using a diverse set of inbred lines and the line × tester method was applied to identify best performing lines and to group QPM inbred lines into different heterotic groups. The test crosses, developed by following line (66) × tester (CML 161 and CML 165) mating design, were evaluated during winter 2013, rainy 2014 and 2015 seasons at Begusarai and Ludhiana, respectively. Based on the specific combining ability, the lines were categorized into two heterotic groups. Out of 66 novel inbreds, 18 lines with significant SCA with CML165 were classified in group A, 16 inbreds with significant SCA with CML161 were classified in group B and 20 inbreds with significant GCA were classified in group (AB). Nine inbred lines were selected based on their positive GCA values and pedigree crosses were developed in rainy season in 2017. Three crosses were made in heterotic group A and four crosses were in group B for synthesizing new inbred lines by using pedigree method. Heterotic grouping based inbred evaluation trial and biochemical analysis were carried out to estimate per se yield potential of developed lines and to estimate tryptophan content. QIL-4-2491 (Group-A) and QIL-4-2401 (Group-B) were the top yielders. A total of 25 crosses were made among the heterotic groups (A and B) by using 22 lines from groups A and B and three best performing hybrids were identified.

Study on pharmacokinetic and tissue distribution of hyperin, astragalin, kaempferol-3-O-β-D-glucuronide from rats with multiple administrations of Semen Cuscutae processed with salt solution with effect of treating recurrent spontaneous abortion.

Semen Cuscutae is a traditional Chinese medicine (TCM) that tonifies the kidneys and prevents miscarriage. According to Chinese medicine theory, kidney deficiency is one of the main causes of recurrent spontaneous abortion (RSA). The previous studies showed that raw product of Semen Cuscutae (SP) and Semen Cuscutae processed with salt solution (YP) have ameliorative effects on RSA, and that YP is superior to SP. However, the active components of YP to ameliorate RSA remain unclear and require further studies. The objective of this study is to investigate the active components of YP in ameliorating RSA. First, a rat model of RSA was established using hydroxyurea in combination with mifepristone. Aqueous decoction of YP was given by gavage to rats. Second, pregnant rats were sampled on days 5, 7, 9, 10 and 12 during the modelling period. The content of Hyperin (HY), astragalin (AS) and kaempferol-3-O-β-D-glucuronide (KA) in blood and liver, heart, spleen, lung and kidney tissues were detected by liquid chromatography-mass spectrometry (LC-MS). The pharmacodynamic indicators including progesterone (P), chorionic gonadotropin β (β-HCG), estradiol (E2), tumor necrosis factor-α (TFN-α), interleukin 4 (IL-4), and tryptophan (TRP) were measured by enzyme-linked immunosorbent assay (ELISA) Pearson's correlation analysis and grey relational analysis were used to establish the relationship between the pharmacodynamic indexes and chemical constituents. The pharmacokinetic results showed that the area under curve (AUC) value of KA was the largest. The tissue distribution results showed that astragalin was widely distributed in liver, heart, spleen, lung and kidney in the RSA model rats, while HY was detected only in the uterus, and KA was detected only in the kidney. The pearson correlationl analysis showed that KA was significantly and positively correlated with the contents of E2, P, β-HCG and TRP. Both AS and HY were significantly negatively correlated with the content of TNF-α, respectively. This study reveals the pharmacokinetics and tissue distribution of KA, AS and HY in rats with RSA. It was elucidated that all three were involved in the regulation of progesterone levels and immune function. It initially revealed the mechanism of action of YP in enhancing the improvement of RSA, and it provided a theoretical basis for the quality assessment of YP.

Insights on optimizing landfill site selection inspired by co-fermentation of weathered coal and landfill leachate

To develop new methods for transforming waste resources such as landfill leachate and weathered coal into clean energy, this research analyzed the co-fermentation effect and internal mechanism of methane production through gas chromatography, three-dimensional fluorescence, inducive coupling and metagenomics techniques. Our findings revealed that landfill leachate addition drives more than 70% increase in weathered coal biomethane production. Anaerobic fermentation collectively increased the tryptophan content in liquid-phase biodegradation, with the highest proportion reaching up to 72%. Paracoccus, Ralstonia, Aquamicrobium and other major microorganisms were in the range of 9–32%. Contamination of heavy metal diversity resistance genes has impacted microflora composition, with specialized heavy metal resistance genes such as NreB, CopD, NreB, and MerP altering the influence of heavy metals on anaerobic fermentation. The combined anaerobic fermentation of landfill leachate and weathered coal has expanded clean waste utilization and has broad application prospects.

Effect of moxibustion on plasma amino acid metabolism in rats with knee osteoarthritis.

To observe the effect of moxibustion at "Zusanli "(ST36) on the plasma amino acid metabolism in rats with knee osteoarthritis (KOA), and to explore the amino acid metabolism mechanism of moxibustion in repairing cartilage injury in KOA. A total of 30 SD rats were randomly divided into normal, model and moxibustion groups, with 10 rats in each group. Rats in the model and moxibustion groups were injected with the mixture of L-cysteine and papain into bilateral knee joint cavity to make the KOA model. The moxibustion group received moxibustion at bilateral ST36 for 30 min, once daily for 30 days. At the end of the experiment, the swelling degree of knee joint was calculated, the mechanical pain threshold was measured by the Von Frey filament, the cartilage tissue injury was observed by HE staining, the matrix metalloproteinase-13 (MMP-13) content in the synovial tissue was detected by enzyme-linked immunosorbent assay (ELISA), and the differential amino acid metabolites in plasma were detected and screened by liquid chromatography-mass spectrometry (LC-MS). Compared with the normal group, the model group showed irregular cartilage surface, decreased number of chondrocytes, uneven distribution, and local clusters of chondrocytes；the contour of the tide line was blurred. The degree of joint swelling in the model group was higher than that in the normal group (P<0.01), the mechanical pain threshold was lower (P<0.01), and the content of MMP-13 in synovial tissue was higher (P<0.01). The contents of proline and tryptophan in the model group were down-regulated (P<0.01, P<0.05). Compared with the model group, the cartilage tissue damage and knee joint swelling were decreased(P<0.05), mechanical pain threshold was increased(P<0.05), MMP-13 content in synovial tissue and levels of glutamate and histidine expression were decreased (P<0.01, P<0.05). Moxibustion at ST36 significantly alleviated arthritis-related swelling and pain in KOA model rats, attenuated cartilage damage, and regulated levels of certain plasma amino acid metabolites. Moxibustion may regulate KOA cartilage synthesis and degradation through amino acid metabolic pathways such as proline, tryptophan, glutamate and histidine, exerting anti-inflammatory, analgesic, and protection of cartilage injury effects.

Tryptophan supplements in high-carbohydrate diets by improving insulin response and glucose transport through PI3K-AKT-GLUT2 pathways in blunt snout bream (Megalobrama amblycephala)

The aim of this experiment was to elucidate the metabolic ramifications of tryptophan supplementation in the context of high-carbohydrate diet-feeding, which is important for improving feeding strategies in aquaculture in order to improve fish carbohydrate metabolism. Juvenile blunt snout bream with an initial mean body mass of 55.0±0.5 g were allocated to consume one of three experimental diets: CN, a normal diet with carbohydrate content of 30% (w/w); HC, a diet with high carbohydrate content of 43% (w/w); and HL, a high-carbohydrate diet to which 0.8% L-tryptophan (L-trp) had been added. These diets were fed for 8 weeks, and the effects of the carbohydrate and tryptophan contents of the diets were assessed.Histological analysis using Hematoxylin and Eosin (H&E) and Oil Red O staining revealed that high-carbohydrate intake was associated with abnormal hepatocyte morphology and excessive liver lipid accumulation, which were notably ameliorated by tryptophan supplementation. A significant increase in plasma glucose, glucagon, AGEs (advanced glycation end products), triglycerides, total cholesterol, and a significant decrease in insulin and hepatic glycogen after a high-carbohydrate diet in terms of plasma indices, compared to the control group. Almost all of them were restored to the normal level in the HL group. The present study might preliminarily suggest that tryptophan supplementation ameliorates the imbalance in glucose metabolism of this species induced by a high-carbohydrate diet. Transcriptomics showed that glucose metabolism under high carbohydrate was mainly regulated by the PI3K-AKT signaling pathway. The mRNA expression and protein levels of GLUT2 also varied with this pathway, which would suggest that sustained activation of this pathway with the addition of tryptophan accelerates glucose transport and insulin secretion under high-carbohydrate diet. Subsequent GTT and ITT experiments have also demonstrated that tryptophan improves glucose tolerance and insulin tolerance in blunt snout bream on a high-carbohydrate diet.In conclusion, these findings elucidate the positive regulatory effect of tryptophan on the PI3K-AKT-GLUT2 pathway under a high carbohydrate diet and provide a theoretical basis for the subsequent rational application of high carbohydrate diets in the future.

Effects of Different Protein Sources on Amino Acid Absorption and Plasma Appearance of Tryptophan, Large Neutral Amino Acids, and Tryptophan Metabolites in Pigs

BackgroundAbsorption of tryptophan (TRP) across the gut epithelium is potentially modulated by competing large neutral amino acids (LNAAs), which could affect the appearance of TRP and its metabolites in the bloodstream. ObjectivesThis study aimed to determine, in a growing pig model of an adult human, the absorption of TRP and other LNAAs from the gastrointestinal tract, and plasma appearance of TRP, LNAAs, and TRP metabolites, in response to dietary proteins varying in TRP content. MethodsPigs were adapted for 7 d to each of 4 diets that differed in their protein source and TRP content: 1) alpha-lactalbumin (AL; 9.95 mg TRP/g diet DM), 2) whey protein (6.59 mg TRP/g), 3) casein (3.73 mg TRP/g), or 4) zein (0.14 mg TRP/g). On day 8, pigs were euthanised after a 12-h fast (baseline), or 1, 2, 3, 4, or 6 h after they received a test meal consisting of 45 g protein, or a protein-free meal (n = 6 pigs at each time in each meal group). Tryptophan and LNAA absorption from the small intestine, and appearance of TRP, LNAAs, and TRP metabolites (melatonin, serotonin, kynurenine pathway metabolites), in the portal vein and systemic circulation, were determined. ResultsAL intake resulted in sustained elevated plasma TRP concentrations after an overnight fast. The amount of TRP absorbed was dose-dependently related to protein TRP content (P = 0.028), with fastest rates for pigs fed AL (371 mg/h). Portal and systemic plasma TRP, TRP/LNAA, and the TRP metabolites were highest (P ≤ 0.05) after AL intake, and remained above baseline levels for ∼4 h postprandially. Absorption rates of TRP correlated with postprandial plasma TRP and TRP metabolites (P ≤ 0.05). ConclusionsIn adult humans, postprandial plasma TRP and TRP metabolite concentrations can likely be modulated by the TRP content of the meal.

Aromatic Amino Acid-Dependent Surface Assembly of Amphiphilic Peptides for One-Step Graphite Exfoliation and Graphene Functionalization.

Amphiphilic peptides show great potential for exfoliating graphite and functionalizing graphene. However, the variety of amino acids complicates our understanding of the underlying mechanisms. In this study, we designed four peptides (C6W1, C6W2, C6W4, and C6W6) with different amounts of aromatic tryptophan amino acids and two additional peptides (C6F4 and C6Y4) by substituting tryptophan with aromatic phenylalanine or tyrosine. This allowed us to investigate the processes and mechanisms of graphite exfoliation and graphene functionalization. Using experimental and computational methods, we discovered that peptides containing tryptophan demonstrated higher exfoliation efficiency and increased tryptophan content further improved this efficiency, resulting in more peptide-functionalized graphene layers. Significantly, the primary driving force for the surface-assisted assembly of peptides on graphite is the π-π stacking interaction between the aromatic ring contributed by aromatic amino acids and the hexagonal rings of the graphite surface. This interaction leads to a layer-by-layer exfoliation mechanism. Our research offers valuable insights into peptide design strategies for one-step graphite exfoliation and graphene functionalization in aqueous environments.

Rhizosphere Mortierella strain of alfalfa exerted weed growth inhibition by inducing expression of plant hormone-related genes.

Weeds are significant factors that detrimentally affect crop health and hinder optimal herbage yield. Rhizosphere microorganisms play crucial roles in plant growth, development, and nutrient uptake. Therefore, research focusing on weed control through the lens of microorganisms has emerged as a prominent area of study. The oil-producing fungus Mortierella, which is known for its numerous agricultural benefits, has garnered significant attention in recent years. In this study, we conducted inoculation experiments in a controlled artificial culture climate chamber to investigate the effects of differential hormones and differentially expressed genes in the stems and leaves of Digitaria sanguinalis using Liquid Chromatography Tandem Mass Spectrometry and RNA-seq techniques, respectively. Additionally, Pearson's correlation analysis was used to establish correlations between differential hormones and growth indicators of Digitaria sanguinalis. The results demonstrated that inoculation with Mortierella sp. MXBP304 effectively suppressed aboveground biomass and plant height in Digitaria sanguinalis. Furthermore, there was significant upregulation and downregulation in the expression of genes involved in the synthesis and metabolism of phenylalanine and L-phenylalanine. Conversely, the expression of genes related to tryptophan, L-tryptophan, and indole was significantly downregulated. The addition of Mortierella sp. MXBP304 can influence the gene expression associated with phenylalanine and tryptophan synthesis and metabolism during Digitaria sanguinalis growth, subsequently reducing the relative contents of phenylalanine and tryptophan, thereby directly inhibiting Digitaria sanguinalis growth.

The Role of Salivary Vascular Endothelial Growth Factor A, Cytokines, and Amino Acids in Immunomodulation and Angiogenesis in Breast Cancer.

In this work, we focused on the analysis of VEGF content in saliva and its relationship with pro-inflammatory cytokines and amino acids involved in immunomodulation and angiogenesis in breast cancer. The study included 230 breast cancer patients, 92 patients with benign breast disease, and 59 healthy controls. Before treatment, saliva samples were obtained from all participants, and the content of VEGF and cytokines in saliva was determined by an enzyme-linked immunosorbent assay, as well as the content of amino acids by high-performance liquid chromatography. It was found that VEGF was positively correlated with the level of pro-inflammatory cytokines IL-1β (r = 0.6367), IL-6 (r = 0.3813), IL-8 (r = 0.4370), and IL-18 (r = 0.4184). Weak correlations were shown for MCP-1 (r = 0.2663) and TNF-α (r = 0.2817). For the first time, we demonstrated changes in the concentration of VEGF and related cytokines in saliva in different molecular biological subtypes of breast cancer depending on the stage of the disease, differentiation, proliferation, and metastasis to the lymph nodes. A correlation was established between the expression of VEGF and the content of aspartic acid (r = -0.3050), citrulline (r = -0.2914), and tryptophan (r = 0.3382) in saliva. It has been suggested that aspartic acid and citrulline influence the expression of VEGF via the synthesis of the signaling molecule NO, and then tryptophan ensures tolerance of the immune system to tumor cells.

Atorvastatin improved ulcerative colitis in association with gut microbiota-derived tryptophan metabolism

AimsAtorvastatin is a commonly used cholesterol-lowering drug that possesses non-canonical anti-inflammatory properties. However, the precise mechanism underlying its anti-inflammatory effects remains unclear. Materials and methodsThe acute phase of ulcerative colitis (UC) was induced using a 5 % dextran sulfate sodium (DSS) solution for 7 consecutive days and administrated with atorvastatin (10 mg/kg) from day 3 to day 7. mRNA-seq, histological pathology, and inflammatory response were determined. Intestinal microbiota alteration, tryptophan, and its metabolites were analyzed through 16S rRNA sequencing and untargeted metabolomics. Key findingsAtorvastatin relieved the DSS-induced UC in mice, as evidenced by colon length, body weight, disease activity index score and pathological staining. Atorvastatin treatment reduced the level of pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α). Atorvastatin also relieved the intestinal microbiota disorder caused by UC and decreased the proliferation of pernicious microbiota such as Akkermansia and Bacteroides. Atorvastatin dramatically altered tryptophan metabolism and increased the fecal contents of tryptophan, indolelactic acid (ILA), and indole-3-acetic acid (IAA). Furthermore, atorvastatin enhanced the expression level of aryl hydrocarbon receptor (AhR) and interleukin-22 (IL-22) and further promoted the expression level of intestinal tight junction proteins, such as ZO-1 and occludin, in colitis mice. SignificanceThese findings indicated that atorvastatin could alleviate UC by regulating intestinal flora disorders, promoting microbial tryptophan metabolism, and repairing the intestinal barrier.

ALTERNATIVE NON-TRADITIONAL SOURCES OF FEED PROTEIN AND AMINO ACIDS

The article presents a literature review on the risks of the global problem of fodder protein and protein amino acids in the feeding of agricultural animals and poultry. Alternative ways of solving it are due to the production and use of non-traditional feed raw materials in feed diets. A number of scientists consider atypical agricultural biotechnological products obtained from insects to be one of the alternative sources of fodder protein and amino acids and see prospects in the development of their production. The most common subjects for study in this regard are crickets, locusts, silkworm larvae, flies, worms, etc. Their protein value, amino acid, fatty acid and mineral composition, the content of vitamins, and other biologically active substances are studied. The development of this direction is considered one of the ways to solve a number of urgent environmental and fodder problems on a biological basis. Рurpose of the research was to study the protein value and complete amino acid composition of flour from insects, namely flour from the larvae of the BSF fly, crickets and snails, and the methods of its determining: crude protein by the Kjeldahl method, digestible protein with the help of 0,2% pepsin, and the digestibility coefficient, and the principles of conducting researches, as well as the characteristics of methods for determining the content of replaceable and essential amino acids, including sulfur-containing, using the Kapel-105M capillary electrophoresis system. As a result of the research, it was found that cricket flour is the least valuable in terms of protein value (digestible protein and digestibility coefficient), and it is also poor in the content of nonessential and essential amino acids, compared to other studied types of insect flour. The best indicators of protein value were noted in the flour from BSF fly larvae in terms of the content of crude and digestible proteins, as well as the digestibility coefficient, which is 75.6%, which indicates its good digestibility by the bodies of animals and birds. The highest content of amino acids is contained in the flour from BSF fly larvae. Taking into account the above results of crude protein content, digestibility, and digestibility in this sample, amino acid digestibility will also be higher compared to other flour samples. In addition, flour from BSF fly larvae is rich in sulfur-containing amino acids. The content of tryptophan and histidine, compared to other types of raw materials, is the highest. The studied samples of insect flour contain a complete set of essential and nonessential amino acids, but BSF fly larvae flour and snail flour are more valuable for essential amino acids.

Portulaca oleracea exhibited anti-coccidian activity, fortified the gut microbiota of Hu lambs

Coccidia of the genus Eimeria are important pathogens that cause coccidiosis in livestock and poultry. Due to the expansion of intensive farming, coccidiosis has become more difficult to control. In addition, the continued use of anti-coccidiosis drugs has led to drug resistance and residue. Some herbs used in traditional Chinese medicine (TCM) have been shown to alleviate the clinical symptoms of coccidiosis, while enhancing immunity and growth performance (GP) of livestock and poultry. Previous in vitro and in vivo studies have reported that the TCM herb Portulaca oleracea exhibited anti-parasitic activities. In total, 36 female Hu lambs were equally divided into six treatment groups: PL (low-dose P. oleracea), PH (high-dose P. oleracea), PW (P. oleracea water extract), PE (P. oleracea ethanol extract), DIC (diclazuril), and CON (control). The treatment period was 14 days. The McMaster counting method was used to evaluate the anti-coccidiosis effects of the different treatments. Untargeted metabolomics and 16S rRNA gene sequencing were used to investigate the effects of treatment on the gut microbiota (GM) and GP. The results showed that P. oleracea ameliorated coccidiosis, improved GP, increased the abundances of beneficial bacteria, and maintained the composition of the GM, but failed to completely clear coccidian oocysts. The Firmicutes to Bacteroides ratio was significantly increased in the PH group. P. oleracea increased metabolism of tryptophan as well as some vitamins and cofactors in the GM and decreased the relative content of arginine, tryptophan, niacin, and other nutrients, thereby promoting intestinal health and enhancing GP. As an alternative to the anti-coccidiosis drug DIC, P. oleracea effectively inhibited growth of coccidia, maintained the composition of the GM, promoted intestinal health, and increased nutrient digestibility.

Eutrophication in cold-arid lakes: molecular characteristics and transformation mechanism of DOM under microbial action at the ice-water interface

During freezing periods, nutrients (carbon and organic matter, etc.) are enriched in the water and sediment of lakes in cold-arid regions, leading to potential algal bloom outbreaks and other health risks to the ecosystem. Particularly, dissolved organic matter (DOM) is a critical component of the nutrients and plays an important role in the global carbon cycle. However, the mechanisms of DOM transfer between ice and water remain unclear. This study analyzed the influence of microbial community on DOM composition using 16 s RNA, 3DEEM, and FT-ICR MS in Daihai Lake and Wuliangsuhai Lake in the Yellow River Basin, China. According to the spectral analysis, the content of endogenous organic matter, such as humus, accounted for 40% of the total DOM in water, while the content of tryptophan and tyrosine accounted for 80% of the total DOM in ice. The results of mass spectrometry showed that lignin was the main component, and the content of organic matter in the ice was less than that in the water. Molecular structures of seven DOM coexisting in the lake ice and water were elucidated with adapted Kendrick-analogous network visualization, which clearly illustrates that long-chain DOM molecules are derived from small molecules, while other heteroatoms are complexed with the side groups. The positive correlations between CHO, CHNO, CHOS, CHOS and Actinomyces indicate that DOM actively interacted with the microbial community. 44% of CHO compounds have the same molecular formula in water, the content of CHOS in the water of the two lakes was closed to 7% higher than that in the ice. Meanwhile, DOM dynamically migrate between ice and water via interstitial water because of the solubility changes under microbial transformation, which has been proved by the decrease in the contents of the humus and tryptophan-like substances in the ice from the bottom to the surface and lower contents of carbohydrate and unsaturated aromatic hydrocarbon in the water than the ice. This study helps to predict the composition and structure of DOM during the migration in lakes and provides a scientific basis for environmental remediation with high concentration of carbon.

Developing a Prototype Device for Assessing Meat Quality Using Autofluorescence Imaging and Machine Learning Techniques

Meat quality determination is now more vital than ever, with an ever-increasing demand for meat, especially with a greater desire for high-quality beef. Many existing qualitative methods currently used for meat quality assessment are strenuous, time-consuming, and subjective. The quantitative techniques employed are time-consuming, destructive, and expensive. In the search for a quantitative, rapid, and non-destructive method of determining meat quality, the use of autofluorescence has been employed and has demonstrated its capabilities to characterise meat grades by identifying biochemical features such as the intramuscular fat and tryptophan content through the excitation of meat samples and the collection and analysis of the emission data. Despite its success, the method remains expensive and inaccessible, thus preventing it from being translated into small-scale industry applications. This study will detail the process taken to design and construct a low-cost, miniature prototype device that could successfully distinguish between varying meat grades using autofluorescence imaging and machine learning techniques.