Raw Protein Content Research Articles

Влияние состава подсолнечного шрота на эффективность отделения белковой части сырья от клетчатки методом механического фракционирования

The purpose of this article is to study the effectiveness of mechanical fractionation of sunflower meal. The objects of the study were two samples of sunflower meal of Russian production, by various manufacturers, and with different content of raw protein. Mechanical fractionation included the stages of grinding raw materials and its sifting using a set of sieves. The effectiveness of mechanical fractionation is determined by the mass yields of the fractions and the content of raw protein in the fractions as well as the ratio of the content of raw protein to the content of raw fiber. Based on this definition of effectiveness, the mass yields and the physicochemical composition of the obtained fractions were determined for each of the samples. The quality parameters of the fractions were selected by the content of raw protein and the content of raw fiber. Despite the different content of raw protein in the raw materials (35% in the first sample and 39% in the second sample), the content of raw protein in the high-protein fraction of both samples was similar and amounted to 42% and 43%, respectively. The fiber content in high-protein fractions of the two samples also differed slightly and amounted to 14% in the first sample and 12% in the second sample. At the same time, a noticeable difference in the mass yields of the fractions in two samples was discovered. The output of the high-protein fraction in the first sample (71%) significantly exceeded the output of the high-protein fraction in the second sample (27%). Thus, mechanical fractionation of the first sample was more effective due to the greater yield of the high-protein fraction.

Quality of raw cow’s milk intended for processing and retail sale from Eastern Poland

The study assessed the quality of raw cow’s milk intended for processing and retail sale (n = 595 samples and n = 19 production batches, respectively) and the relationship between the number of somatic cell and the fat and protein contents. All milk samples were tested for total bacteria count, somatic cell count, antibiotic residues, and fat and protein contents. Additionally, raw drinking milk samples were tested for counts of Enterobacteriaceae, Escherichia coli, coagulase-positive staphylococci, enterococci, and the presence of Listeria monocytogenes and Salmonella spp. In raw milk samples, TBC, SCC, and fat and protein contents ranged from 4.9 × 103 to 2.3 × 105 CFU mL–1, from 9.9 to 407.8 thou. mL–1, from 3.2% to 5.9% and from 3.0% to 4.8%, respectively, while TBC, SCC, and fat and protein contents in raw drinking milk samples ranged from 1.8 × 106 to 3.3 × 106 CFU mL–1, from 454 to 696.9 thou. mL–1, from 1.67% to 2.88% and from 3.11% to 3.41%, respectively. The mean counts of Enterobacteriaceae, E. coli, coagulase-positive staphylococci, enterococci in raw drinking milk were 4.0 × 105 CFU mL–1, 1.4 × 102 CFU mL–1, 2.4 × 104 CFU mL–1, and 2.2 × 105 CFU mL–1, respectively. L. monocytogenes, Salmonella spp., and antibiotic residues were not detected in any of the samples tested. The study showed that 95% of the raw milk samples were of satisfactory quality, while 100% of the raw drinking milk samples were of unsatisfactory quality in relation to the regulatory thresholds. Additionally, raw drinking milk samples were a vehicle of potentially pathogenic bacteria. The somatic cell count did not affect the fat and protein content in the analysed milk.

Evaluation of the Mineral, Amino Acid and Antimicrobial Properties of Raw and Fermented Trametes elegans

Mushrooms are known to be sources of food and bioactive compounds with medicinal properties. In this study, the mineral, proximate, amino acid and antimicrobial properties of a wild macrofungus, Trametes elegans was investigated. Standard methods were used to evaluate the mineral, proximate and amino acid content of raw and fermented T.elegans. By soaking in methanol and acetone, extracts from the macrofungus’s powdered fruiting bodies were obtained. Using common microbiological methods, the macrofungus extracts’ antimicrobial activity was assessed on test isolates. In addition, isolation and identification of microorganisms associated with raw and fermented T. elegans was done using standard microbiological techniques. The physicochemical parameters (Temperature, pH and Total Titratable Acid) were also monitored during solid and liquid state fermentation of Trametes elegans. The concentration of carbohydrate (70.24±0.33) was higher in the raw sample, while moisture (3.22±0.13), fat (1.85±0.04) and protein contents (22.48±0.09) were higher in sample subjected to solid fermentation. Also, crude fibre (6.68±0.76) and Ash (3.60±1.39) were higher in sample subjected to liquid fermentation. The micro elements analysed are zinc and iron and macro elements are potassium, magnesium and phosphorus and their values increased in fermented Trametes elegans. Analyses of both essential and non-essential amino acids were conducted. The most common amino acids found in all of the samples examined were glutamic acid, leucine, arginine, and aspartic acid. Cysteine and methionine are the least abundant. The amino acid scores were determined by ratio of the milligrams of amino acid per gram of test protein to the milligrams of amino acid per gram in the reference pattern. The results showed that methionine had the lowest score in the raw sample (3.14) and tryptophan had the highest score (18.17) in T. elegans that had undergone solid fermentation. Methanol and acetone extracts inhibited all test isolates except Lactobacillus plantarum. The commercial drug exhibited higher antibacterial activity compared to the extracts, with a significant difference (P≤ 0.05). The inhibitory zones ranged from 5.50 mm against Bacillus subtilis to 18.13 mm against E. coli. The range of the minimum bactericidal concentration (MBC) was 12.5 to 50 mg/mL, while the minimum inhibitory concentration (MIC) was 12.5 to 50 mg/mL. In light of this findings, T. elegans, could be considered as potential sources of natural antimicrobial and can also be relevant for use as food supplements in animals and man.

GENOME-WIDE ASSOCIATION STUDY FOR GRAIN QUALITY TRAITS IN SPRING BARLEY COLLECTION GROWN IN NORTH KAZAKHSTAN

Barley is a cereal crop that is grown all over the world. Its grain is used for animal feed, malting, brewing, and food. The quality of barley grain is important, particularly raw starch and protein contents, and it depends on the end-use product. This study looked at a collection of 356 barley accessions from the USA and Kazakhstan grown under conditions of northern Kazakhstan (Karabalyk agricultural experimental station) and genotyped with 1631 polymorphic SNPs markers. The collection was studied for starch (GSC), protein (GPC), cellulose (GCC), and lipids contents (GLC), and for grain test weight (TWL) during two years. Phenotypic analysis demonstrated impact of the year on studied traits and significant associations between grain quality and the yield (P < 0.01). Population structure analysis revealed three subclusters in the studied barley collection with the dominance of the USA’s barley in two of them. As a result of GWAS, 22 significant QTLs (P < 0.001) were identified for the studied grain quality traits including 19 single-trait QTLs, 2 double-trait QTLs, and a one triple-trait QTL. For 16 QTLs, reference quality genes and/or QTLs were found, while the remaining 6 QTLs were presumably novel genetic factors for grain quality traits. As result, these 22 QTLs are expected to be useful for future breeding projects targeting the selection of high grain quality barley cultivars.

Ameliorating Forage Crop Resilience in Dry Steppe Zone Using Millet Growth Dynamics

Introducing new forage crops such as Japanese millet (Echinochloa frumentacea) and pearl millet (Pennisetum glaucum) is crucial for mitigating the impacts of climate change in the dry steppe zone, expanding forage crop options, and obtaining nutritious feed for the development of animal husbandry. The aim of this study was to assess the productivity and feed value of these crops. Field experiments were conducted in 2021 and 2022 to investigate the changes and variations in the yields and chemical compositions of Japanese millet and pearl millet when grown as sole crops or in mixed cropping in the dry steppe zone of northern Kazakhstan. Among the annual crops sown via sole cropping, the hay of the Japanese millet, sown in the third decade of May and harvested during full heading, was observed to have a higher content of raw protein and other nutrients than Sudanese grass hay, that is, the raw protein content was higher at 1.81%, the raw oil content at 0.12, and the raw ash content at 1.88%. In addition, among the mixtures of crops, the hay of the crop mixtures containing pearl millet, sown in the above period and harvested as hay during the milky stage, the full heading stage, and the formation of spikelets in the grain family stage, differed from the hay of Sudanese grass sown in the same period and collected during full heading in terms of the contents of raw protein and other nutrients and the low content of raw ash, that is, the content of raw protein was at 2.16%, raw oil at 0.39, raw ash at 0.95, without nitrogen extractives (WNEs) at 3.78, and starch at 0.11. The calcium content was higher by 0.08% and carotene by 0.11 mg/kg, and raw lentils were lower by 0.94%. The analysis of the results revealed that the variation in the crops’ phenological phases depended on the moisture availability and the sowing time.

CULTIVATION OF VARIABLE LUCERNE VARIETY VICTORIA IN MONOCULTURAL AND POLYCULTURE AGROPHYTOCENOSIS UNDER THE CONDITIONS OF UDMURT REPUBLIC

Variable lucerne is a valuable perennial legume crop known for its high fodder productivity, elevated raw protein content (up to 24%), drought resistance, winter hardiness, extended utilisation period, and the ability to maintain soil fertility. This crop is cultivated in pure stands and grass mixtures with other legumes and grasses. In 2016, a new variety of variable lucerne, Victoria, was included in the State Register of Breeding Achievements and approved for use in the Volga-Vyatka, Ural, West Siberian, North-Western, Central Chernozem, East Siberian, and Middle Volga regions of the Russian Federation, with the FSBI «UFARC UB RAS» (Federal State Budgetary Institution "Ural Federal Agrarian Research Center Ural Branch of the Russian Academy of Sciences") as the originator. The research was conducted in 2019–2022 to study the fodder productivity and nutritional value of monocultural and polyculture agrophytocenosis with the Victoria variety of variable lucerne on derno-podzolic soils in the Udmurt Republic. It was found that variable lucerne made the main contribution to agrophytocenosis yield over three years, accounting for 56–87% in two-component grass mixtures and 44–69% in three-component ones. The prevalence of Festuca rubra and Festuca pratensis in mixed crops increased from 18–26% in the first year of use to 32–43% in the third year. The highest yield (7.3 t/ha) of dry matter was obtained from the grass mixture of variable lucerne and red fescue at a stem density of 727–951 pcs/m2, with variable lucerne accounting for 74–80% and red fescue for 11–21%. Among lucerne-grass agrophytocenoses, the mixtures with Festuca pratensis were the most productive, yielding 6.3–t/ha of dry matter. The feed value of the selected grass mixtures was high: in 1 kg of dry weight, there was 17.4–21.3% raw protein, 0.69–0.76 feed units, and 9.2–10.0 MJ of metabolisable energy.

Nutraceuticals with high antioxidant properties based on fruits and medicinal plants

Recently, consuming natural food supplements derived from natural resources has become widespread in many developed societies due to the lack of side effects, low cost, and high antioxidant properties to prevent and treat many diseases. The research and formulation aimed to design a safe and effective medicinal and edible healthy natural nutraceutical with high antioxidant properties and rich mineral elements based on fruits and medicinal plants. Antioxidant, Protein, and inorganic contents of fruits and natural herbal plants were determined to evaluate the contents of active constituents of the meal in milligrams. The results were then used to formulate an edible herbal supplement as a solid oral system. The raw protein content of each ingredient was evaluated through the Kjeldahl method. The contents of the nutrient elements were measured in mg/kg (D.W) by ICP-Mass. All components were mixed thoroughly in some proper percentages, milled, and then dried by heating for 48 hours. The Flowability of the powder was subsequently evaluated using the work index and Hausner ratio. The work index of the sample was 34.3, and because the Flowability was not optimal, aerosol was added to 10 grams of a mixture of 1% by weight of the material. Finally, the working index reached 23, which is desirable. Furthermore, the ingredients were volumetrically encapsulated in OO-sized capsules and subjected to quality control tests. Performing organoleptic assays for controlling the physical and chemical properties of the formulation, the active ingredient was determined in terms of the results of Kjeldahl and inductively coupled plasma Mass spectrometry (ICP-Mass). The results of all control tests, including the weight deviation test, opening time test, and active ingredient dosage test, were conducted for the developed formulation according to credible pharmacopeia. According to the allowable daily dosages, the developed capsule is a proper herbal supplement with very high antioxidants.

Investigation of the Physiological Effects of Plant Conditioners in Field Experiments of Winter Wheat

Our study aimed at to test the effects of two different plant conditioners on some morphological parameters, yield and grain quality of winter wheat (Babona) in a field experiment with medium plots, and to investigate some physiological parameters closely related to yield quantity and quality at the beginning of ear emergence by in vivo field measurements. At the beginning of ear emergence we determined the relative chlorophyll content of leaves (SPAD value), moreover leaf reflectance was measured with a portable spectroradiometer to produce spectral vegetation indices that can be used to evaluate the effects of treatments on chlorophyll, carotenoid, anthocyanin and water content of leaves, and to infer photochemical efficiency and stress sensitivity. The results were strongly influenced by the unfavourable rainfall distribution during the growing season. Precipitation deficit in spring significantly reduced the development of the crop stand (yield was below the national and county levels), and its effect was also observable in quality parameters (low raw protein and gluten content), but the positive effect of treatment 1 was detectable: higher yield and quality, higher plant height and ear length compared to the control. At the beginning of ear emergence, some spectral vegetation indices indicated the positive effect of treatment 1 despite the drought: chlorophyll content and photochemical activity of leaves were higher, and higher stress sensitivity and protective pigment concentration in the control.

Testing of winter wheat (Triticum aestivum L.) varieties of ASC "Donskoy" on chestnut soils of the Volgograd region

In Russia, the main food culture is wheat. Its cultivation is a guarantor of stability and efficiency of the economy. A significant role of breeding work is in increasing its productivity. Therefore, this research was conducted during 2020-22 at the Federal Scientific Center of Agroecology of the Russian Academy of Sciences in Kamyshinsky district for testing winter wheat varieties. These varieties were selected as the most stress- and drought-resistant in their breeding characteristics. The location and laying of the experimental plot were selected so that the characteristics of the varieties corresponded to climatic indicators, field records of the yield structure were conducted according to the methodology of B. A. Dospekhov and the state variety testing. The Pearson coefficient (r-Pearson) was used for the correlation analysis. The significance of the differences was assessed by the smallest significant difference at the significance level of 5% (НСР05). According to the totality of the variety's reactions to the complex of prevailing soil and climatic conditions, it is possible to distinguish the varieties Razdolye and Premier. The highest yields were shown by Lilit, Don's Niva, Premier and Don's Ruby varieties, from 2.50 to 2.66 t/ha. The analysis of winter wheat grain for the content of raw gluten and protein allowed us to identify the highest quality varieties: Dione and Onyx–24.1-25.6% and from 13%, respectively. Lilit, Don's Niva, Don's Ruby and Premiere varieties showed great profitability. Statistical processing showed that the grain yield of the studied winter wheat varieties in the steppe zone was reliably associated with field germination (r=0.5-0.7), grain weight in the ear (r=0.7), weight of 1000 grains (r=0.53) and the number of grains in the ear (r=0.68). Thus, in the conditions of the Lower Volga region, the quantitative values of weather factors were identified that determine the formation of high yields of varieties: Expanse, Premiere.

Identification of SNPs Associated with Grain Quality Traits in Spring Barley Collection Grown in Southeastern Kazakhstan

Barley (Hordeum vulgare L.) is an important cereal crop with high genome plasticity that is cultivated in all climatic zones. Traditionally, barley grain is used for animal feed, malting, brewing, and food production. Depending on the end-use product, there are individual requirements for the quality traits of barley grain, particularly for raw starch and protein contents. This study evaluates a collection of 406 two-rowed spring barley accessions, comprising cultivars and lines from the USA, Kazakhstan, Europe, and Africa, based on five grain quality traits (the contents of raw starch, protein, cellulose, and lipids, and grain test weight) over two years. The results of population structure analysis demonstrate the significant impact of geographical origin on the formation of subclusters in the studied population. It was also found that the environment significantly affects grain quality traits. Heat and drought stresses, particularly during grain filling, led to higher protein and lower starch contents. A genome-wide association study (GWAS) using a multiple-locus mixed linear model (MLMM) allowed for the identification of 26 significant quantitative trait loci (QTLs) for the five studied grain quality traits. Among them, 17 QTLs were found to be positioned close to known genes and previously reported QTLs for grain quality in the scientific literature. Most of the identified candidate genes were dehydration stress and flowering genes, confirming that exposure to heat and drought stresses during grain filling may lead to dramatic changes in grain quality traits, including lower starch and higher protein contents. Nine QTLs were presumably novel and could be used for gene mining and breeding activities, including marker-assisted selection to improve grain quality parameters.

Aftergrass ability of grass stands for hay-pasture use in the autumn period

Aim. To identify the best types of perennial grasses in terms of fertility in their pure form and mixtures, and to develop a technology for the formation and use of sown meadow phytocenoses in the Forest Steppe during their autumn alienation in the system of an extended pasture conveyor for meat breeds of cattle. Methods. Field, laboratory, mathematical, system analysis. Results. The article on onion growing considers the problems of quality provision of meat cattle with green fodder not only in the summer, but also in the autumn period. On the basis of many years of experimental research, the article reveals the processes of formation of perennial grasses depending on the weather and climate conditions, species composition, fertilization system, and the timing of the removal of the pasture mass in the fall. The indicators of the transformation of the quality of the green mass of аftergrass depending on the terms of deepening of the autumn period are given. According to the results of field research, it was established that early-ripening and medium-ripening perennial grasses in the autumn period form a sufficient supply of grass at the root (oats) for livestock grazing, in the range of 2.3-3.1 t/ha with moderate moisture supply and application of nitrogen fertilizers with a dose of N20 under the pasture moving. Early-ripening and medium-ripening perennial grasses produce a 1.4–1.6 times higher yield than late-ripening coenoses over the years of use. This pattern was stable over the years of use. Аftergrass cereal grasses of the autumn period were of sufficient quality and met the zootechnical standards for feeding cattle of the meat sector. The supply of green fodder with organic nutrients and mineral elements for the cultivation of аftergrass on the background of N20 was characterized by the following indicators: the content of raw protein in the dry weight of the fodder was in the range of 11–15.3%, fat – 2.4–3.1%, raw fiber – 27.7–35.3%, potassium – 2.39–2.75%, calcium – 0.51–0.68%. The digestibility of cereal grass fodder was 58-72%. Conclusions. Among the perennial grasses, the following types of grasses showed better seeding in the autumn period on dry meadows with the application of nitrogen fertilizers with a dose of N20: thornless sedge, oriental sedge and ryegrass, the share of which in the yield of seeded grass mixtures depends on the intensity of precipitation after 3 years of use was at the level of 62–71%, and in some dry years even more.

The assessment of blue lupine varieties according to productivity and adaptability in the conditions of Kirov region

The article provides the results of the comparative study of grain productivity of 7 blue lupine (Lupinus angustifolius L.) varieties bred by the All-Russian Scientific Research Institute of Lupine in the conditions of Kirov region on sod-podzolic light loamy soil. It has been established that the yield of the seeds of blue lupine varied 1.5-2 times over the years of cultivation (2000-2022) depending on the weather conditions. Over 3 years on the average the varieties Bryansky kormovoy and Siderat were the most productive, 46-287 and 281 g/m2, respectively. Even in extremely unfavorable for blue lupine 2022 (by environment condition index of 62) these varieties significantly exceeded the control (Vitayz – 135.8 g/m2) in yield, by 72.9 and 61.1 %. The coefficient of adaptability of the varieties Siderat 46, Smena, Uzkolistny 53, Bryansky kormovoy was over 100 %. The productivity structure elements of blue lupine, namely: the number of beans per plant, the number of grains per bean and mass of 1000 seeds depended on hydrothermal conditions of the growing period. Under favorable conditions the number of beans per plant and the size of seeds increased. The number of grains per bean was the most stable indicator. The average duration of the growing period of lupine varieties was 84-91 days. In hot and dry weather of 2022 the ripening began 5-7 days earlier than long-time average annual periods. The varieties with highly developed side branching had a longer growing period – Bryansky kormovoy, Uzkolistny 53 and. Vitayz. During the year favorable by hydrothermal conditions the raw protein content in lupine seeds was 30.8-36.5 %. In unfavorable years it decreased by 20-45 % depending on the variety.

Productivity of buckwheat in 6-field crop rotations in the conditions of the Left Bank Forest Steppe

Aim. The purpose of the study is carried out in the established structure of influence, recruitment and placement of field crops in various crop rotations on overall productivity, yield and quality of buckwheat in particular. The study was carried out in the subzone of unstable moistening of Left Bank Forest-Steppe of Ukraine on typical low-humus Panfilska chernozem research station of the NSC «IZ NAAN». Methods. Growing technology of agricultural crops in experiments is generally accepted and recommended for the research area. Chemical means of protection were used plants for the production of competitive marketable buckwheat products. Results. Studies conducted in a long-term inpatient setting field research, which was established in 2001, on the study of crop rotations on typical ones chernozems of unstable moistening of the Left Bank Forest Steppe, convincingly testify to the fact that during 2016–2020 the research is the most 6-field crop rotations are rational in this zone: soybeans – winter wheat. – sugar beets – spring barley – corn for grains – buckwheat; buckwheat – winter wheat – soybean – spring wheat – winter rye – spring barley for organic of the mineral intensification system (by-products of the precursor + N40P40K40 for buckwheat). Conclusions. Buckwheat yield, which was grown after corn for grain and spring barley as precursors and corresponding set crop rotation, was in the range of: 2.05–2.10 t/ha, while the content of raw protein was contained at the level of 14.7–15.0%. Economic efficiency growing buckwheat in the structure of 6-field crop rotation becomes: conditionally net income – 13.89–14.48 thousand hryvnias/ha for a high level profitability – 130–135%.

ҚҰС САҢҒЫРЫҒЫНАН ЖАСАЛҒАН ОРГАНИКАЛЫҚ ТЫҢАЙТҚЫШТЫҢ ӘРТҮРЛІ ДОЗАЛАРЫНЫҢ МАЙЛЫ ЗЫҒЫР МЕН АРПА ТҰҚЫМЫНЫҢ ТЕХНОЛОГИЯЛЫҚ САПАСЫНА ӘСЕРІ

This article studied the effect of different doses of organic fertilizers from poultry manure on the technological quality of barley and oilseed flax seeds. The technological properties of the seeds were determined in accordance with generally accepted methods. All the obtained results were subjected to one-factor variance statistical processing. Under the influence of various doses of organic fertilizers, the protein content in flax seeds increased by 10-12% relative to the control and showed a statistically significant positive result (F: 51.082; p<0.05). The fat content of flax increased by 0.41-0.605% compared to the control. The effect of organic fertilizer on the iodine number and the amount of polyunsaturated linolenic and linoleic acids of flaxseed was not observed. 5 t/ha of bird droppings was selected as the dose that best affected the technological quality of flax seeds. The content of raw protein in dry matter in barley seeds increased by 0.37% compared to the control variant, the fat content - up to 1%. The mass fraction of ash showed an increase relative to the control by 8.1-18.8%. The increase in the quality of barley seeds was most optimally influenced by a dose of bird droppings of 15 t/ha. The effect of various doses of organic fertilizers from poultry manure on the mass fraction of crude fiber, on the indicators of nitrogen-free extractives was determined within the control variant.

Influence of Water Deficit on the Chemical Composition of Soybean Grains

With relation to the present issue about the influence of the periodic water deficit on the content and yield of the main chemical components, summarized annual data have been used including years of different characteristics, with droughts in different vegetation phenophases of soybean: very dry, averagely dry and average. The experiment was conducted at the Agricultural University of Plovdiv. The experiment was set in 4 repetitions with a size of experimental plots - 30 m 2 , and the crop plots - 10 m 2 . Criteria for watering performance was pre-watering moisture of soil at option 5-80% under-soil moisture for the layer 0-60 cm. The irrigation norm for all options was 50 mm. The irrigation norm for option 5 was 150 mm, and for options 2, 3 and 4-100 mm. Irrigation was performed gravitative along short closed furrows. After the completion of all experimental options, there were established the content and yield of the main chemical components in soybean grains - raw fat content, raw protein, raw fibres, raw ash and non-nitrogen extracted substances. Watering cancellations in the period of seed filling decrease the raw protein content. Watering cancellation in the bean formation period has a negative influence on protein content in soybean or does not influence it at all. Watering cancellation during the reproductive period decreases raw protein yield, which leads to a decrease in grain yield. Regarding raw fats, the most favourable is the water cancellation in the period of seed filling which favours growth in their content. Watering cancellation in the period of seed filling leads to a more significant decrease in lysine content. Despite the watering cancellation in a particular phase, the application of the other two waterings increases carbohydrate content in soybean grains compared to that obtained in non-watering conditions.

PREPARATION AND EVALUATION OF THE FUNCTIONAL PROPERTIES OF PROTEIN ISOLATES AND HY-DROLYSATES FROM PLANT RAW MATERIALS

Animal protein plays a key role in the human diet as the most balanced amino acid composition; however, its consumption often causes allergic reactions. Plant protein serves as a substitute for animal protein. The most promising sources of plant protein are the seeds of cereals, pulses, oilseeds and cereals. Research aim: selection of conditions for obtaining protein isolates and enzymatic hydrolysates having the desired functional properties from different types of vegetable raw materials.Pea, corn and oat flour LLC "FavoritT"; linseed flour LLC NGO "Compass Health". Enzyme preparations: chymotrypsin LLC "Samson-Med"; Protex 40E Genencor; protosubtilin G3x produced by PO "Sibbiofarm" LLC; pancreatin PJSC "Biosintez"; trypsin LLC "Diaem"; beef pepsin OJSC "MHSF". Crude protein content was determined by Kjeldahl method, protein substances - by modified Lowry method. Fat-holding, water-holding, emulsifying and foaming capacities, as well as allergenicity of protein isolates and hydrolysates were determined. Conditions for protein substances extraction from flax, corn, oat and pea flour with the yield of high-molecular protein fraction not less than 70 % of raw protein content were selected. The conditions of protein isolates precipitation to produce preparations containing not less than 85% of protein have been selected. The type of enzyme preparation for hydrolysis - pancreatin - was selected. It was found that in order to increase water- and fat-holding capacity of pea isolate, hydrolysis is possible with duration not exceeding 15 min, for all other isolates hydrolysis is undesirable. The best emulsifying and foam-forming capacities are possessed by linseed hydrolysates after 60 and 90 min of hydrolysis, respectively. Enzymatic hydrolysis was shown to reduce the allergenicity of plant proteins. The obtained hydrolysates of vegetable proteins can be used as ingredients for functional products, as well as for obtaining products with reduced allergenicity.

Yields and grain quality of winter rye cultivated on sod-podzolic radioactively contaminated soil depending on the applied means of chemicalization

Relevance. The effect of mineral fertilizers of various degrees of saturation and the biological preparation Albit on the yields and quality of winter rye grain of the Moskovskaya-12 variety has been studied in a stationary field experiment on the radioactively contaminated sod-podzolic sandy loam soil. Methods. The researchers conducted (2019–2021) at the experimental field of the Novozybkov branch of the Bryansk State Agrarian University established that the highest yield of winter rye grain (4.37 t/ha) was formed when using full mineral fertilizer N90P60K150 against the background of treatment of winter rye plants with biopreparation Albit. Results. Under the influence of the applied means of chemicalization, an improvement in the biophysical parameters of winter rye grain has been noted. The content of raw protein in winter rye grain, depending on the fertilizer systems used, has varied from 11.3 to 13.5% with its highest collection (0.599 t/ha) per unit area when using the N90P60K150 fertilizer system in combination with the biopreparation Albit. The use of the complete mineral fertilizer (NPK) with consistently increasing doses of potassium both separately and in combination with the biopreparation Albit has contributed to a decrease in the specific activity of 137Cs in winter rye grain by more than 2.1 times in comparison with the control.

Microbial conversion of syngas to single cell protein: The role of carbon monoxide

Syngas has been widely utilized as substrate in microbial processes to produce various high-value products. Nevertheless, its applicability for single cell protein (SCP) production by hydrogen-oxidizing bacteria (HOB) has never been explored. Especially, the role of carbon monoxide in SCP synthesis is unknown. Thus, this study first investigated the effect of different CO and H2 ratios in syngas on Cupriavidus necator H16 growth and SCP production. The growth of C. necator H16 was significantly restrained with the increase of CO content, and it almost ceased once the CO:H2 ratios were above 1. In addition, the growth rate of C. necator H16 increased with the increase of shaking frequency and inoculum volume. Furthermore, larger gas–liquid interfacial areas and gas input amount resulted in a higher growth rate and OD value. The raw protein contents were around 50–60% regardless of CO concentration, and the amino acid profiles showed no apparent differences. The results showed that although the presence of CO led to the reduction of the growth rate of C. necator H16, it had a limited effect on the SCP quality. H2 and CO2 in syngas were the primary substrates for SCP production, while CO served more as a toxic inhibitor. The successful demonstration of SCP synthesis from syngas may provide a superior route for the valorization of waste biomass by turning it into food or feed.

The Impact of Hydroponic Feeding Stuff with Antibiotic Action on Egg-laying Capacity

The article describes the study of poultry feeding by adding fenugreek, liquorice, amaranth grains, having antibiotic action and hydroponically cultivated grains of corn, barley, amaranth and fenugreek to the feed allowance of chickens, having high nutritional qualities and influence on the egg-laying capacity of maternal chickens. The studies were carried out in the Khudat poultry farm of the Khachmaz district and on parental chickens, rearing at the Veterinary Scientific Research Institute. So, from antibiotic-effective plants in order to study the impact of fenugreek, liquorice, amaranth grain and cultivated hydroponically of corn, barley, amaranth and fenugreek grains having high nutritional qualities on the egg-laying capacity of chickens by adding to the feed allowance, an experiment was set. The feeds mentioned have antibacterial and antitoxic properties and regulate water-salt metabolism, contribute to the normal functioning of the stomach, stimulate the peristalsis of the gastrointestinal tract, strengthen the endocrine and immune system, the body's resistance. As a consequence of the studies conducted, it was found that the content of raw protein in amaranth grain grown by the hydroponic method is - 23.1%, and in fenugreek - 33.9%, as well as 19.9% of linolenic acid contained in the seeds mentioned, by reducing the level of the cortisol stress hormone which can destroy muscles and increase the fat layer, strengthens the immune system. As a result, the transport of nutrients to the cells improves, which makes it easier to absorb nutrients, increases bone density by improving the balance of calcium.

Blue lupin (Lupinus angustifolius L.) ‒ the results of varieties and accessions study according to adaptivity and a set of commercialbiological traits

Cultivation of grain legumes including blue lupin (Lupinus angustifolius L.) is an important reserve for increase of high protein feed production. The aim of the research is to find perspective blue lupin varieties and accessions according to yield, adaptivity, the duration of vegetation period and biochemical indices in south-western area of the Central part of Russia. In 2019-2021 six blue lupin varieties and four accessions developed in the All-Russian Lupin Scientific Research Institute have been tested in competitive variety trial (Vityas was standard variety). The trials were laid on sod-podzolic, loamy soil with the moderate fertility level. The average grain yield of varieties in the trial was 2.07 t/ha, of standard variety – 1.85 t/ha. The varieties Bryansky kormovoy, Uzkolistny 53 and accessions BSv 51-19, USN 53-236 and SBS 56-15 had the highest grain yield. Their grain productivity varied from 2.17 to 2.29 t/ha. Statistically significant increase to the standard was 0.32-0.44 t/ha. The accessions BSv 51-19 and USN 53-236 had the maximum grain yield by adaptivity coefficient of 110%. According to the green mass yield and adaptivity Bryansky kormovoy and new promising accessions SBS 56-15, USN 53-236 and BSv 51-19 have been noted: the increase to the standard according to the green mass yield was 5.0-7.0 t/ha, the adaptivity coefficient was 104-110%. By the duration of vegetation period (80-89 days) all varieties and accessions were included into the group of early-ripening. The new tall variety Belorozovy 144 had the longest vegetation period (89 days). According to the alkaloid content in the grain (0.031-0.063 %) all tested varieties and accessions were included into the low-alkaloid group. The varieties Smena, Uzkolistny 53 and the accession SBS 56-15 were characterized by stable low index (0.031-0.039 %). The content of raw protein in the seeds of tested varieties and accessions of blue lupin varied in the range of 32.0-33.8%. According to the set of positive traits, Bryansky kormovoy variety and accessions USN 53-236 and SBS 56-15 have been noted.