Oil Content In Rapeseed Research Articles

Investigation of the quality of rapeseed oil derived from different varieties and growth periods based on GC-IMS technique

Rapeseed oil is consumed worldwide, and its quality is closely related to the raw rapeseed material. In this work, the quality and flavor differences of rapeseed oil from twelve varieties with two growth periods were studied. The results showed that different varieties responded differently to changes in growth periods, with the GXY1 variety having the greatest combined effect. Shortening the growth period may reduce the moisture and protein content and increase the oil content of rapeseed. It may also enhance the oleic acid content and decrease the linoleic acid content. A total of 62 aroma compounds were identified by gas chromatography-ion migration spectrometry (GC-IMS), with aldehydes being the main flavor compounds. The flavor fingerprint of rapeseed oil derived from different varieties was effectively constructed. Seven key aroma compounds with relative odor activity value (ROAV) > 1 were identified, which significantly influenced the flavor of rapeseed oil. This work may provide new insights for selecting favorable rapeseed varieties for industrial production of high-quality rapeseed oil.

Determination of flammability indicators of multicomponent biofuel in diesel

The object of research — multicomponent biofuel compositions, including 15 and 25 % rapeseed oil and ethanol, up to 100 % diesel fuel: MCBFC-15, MCBFC-25. The research objective is to study the effect of different content of rapeseed oil and ethanol additives in the MCBFC on their flammability indicators. The purpose of the study is the analytical determination of the cetane number and the ignition delay period in a diesel engine during its operation on MCBFC-15, MCBFC-25. Research methods — empirical analysis and generalization, development of a mathematical model. Keywords diesel fuel, rapeseed oil, ethanol, multicomponent biofuel composition (MCBFC), cetane number, ignition delay period

Fine mapping and candidate gene analysis of a major QTL for oil content in the seed of Brassica napus.

By integrating QTL fine mapping and transcriptomics, a candidate gene responsible for oil content in rapeseed was identified. The gene is anticipated to primarily function in photosynthesis and photosystem metabolism pathways. Brassica napus is one of the most important oil crops in the world, and enhancing seed oil content is an important goal in its genetic improvement. However, the underlying genetic basis for the important trait remains poorly understood in this crop. We previously identified a major locus, OILA5 responsible for seed oil content on chromosome A5 through genome-wide association study. To better understand the genetics of the QTL, we performed fine mapping of OILA5 with a double haploid population and a BC3F2 segregation population consisting of 6227 individuals. We narrowed down the QTL to an approximate 43kb region with twelve annotated genes, flanked by markers ZDM389 and ZDM337. To unveil the potential candidate gene responsible for OILA5, we integrated fine mapping data with transcriptome profiling using high and low oil content near-isogenic lines. Among the candidate genes, BnaA05G0439400ZS was identified with high expression levels in both seed and silique tissues. This gene exhibited homology with AT3G09840 in Arabidopsis that was annotated as cell division cycle 48. We designed a site-specific marker based on resequencing data and confirmed its effectiveness in both natural and segregating populations. Our comprehensive results provide valuable genetic information not only enhancing our understanding of the genetic control of seed oil content but also novel germplasm for advancing high seed oil content breeding in B. napus and other oil crops.

A stable quantitative trait locus on chromosome A10 improves the oil content of a backbone parent in Brassica napus L.

One of the most important breeding goals for rapeseed (Brassica napus L.) is improving the oil content (OC) to increase edible or industrial oil quality. Using backbone parents as research materials to excavate OC genetic loci is not only helpful in enhancing the understanding of the genetic basis of OC in rapeseed but also offers a promising approach to improve backbone parents. In this study, a doubled haploid population constructed using the backbone parent 19514 A (with low OC) and the inbred line ZY50 (with high OC) were investigated in eight environments. A total of 30 quantitative trait loci (QTLs) were identified, among which qOC.A10 was detected in six environments. Local QTL scanning for the BC4F2 population showed that qOC.A10 accounted for 22.77% of the phenotypic variance and 0.97% of the additive effect. Further, we improved the parent 19514 A on qOC.A10 by marker-assisted selection, and the background response rate of the improved line A10–19514A reached 97.5%. The phenotypic identification results showed that the OC of A10–19514A and its hybrid significantly increased compared with that of the control, with no negative effects on other agronomic traits. Transcriptome analysis and sequence difference analysis facilitated the prediction of potential functional genes of qOC.A10. This study increases the knowledge regarding the genetic foundation of OC in rapeseed. Furthermore, it identified and confirmed a stable QTL that can be detected in various studies and possesses practical breeding significance.

Migration and transformation of Cd in four crop rotation systems and their potential for remediation of Cd-contaminated farmland in southern China

A crop rotation system combining agricultural production with phytoremediation is an economical and sustainable method of remediation of cadmium (Cd)-contaminated farmland. This study focuses on migration and transformation of Cd in rotation systems and the influencing factors. In a two-year field experiment, four rotation systems were evaluated: traditional rice and oilseed rape (TRO), low-Cd rice and oilseed rape (LRO), maize and oilseed rape (MO), and soybean and oilseed rape (SO). Oilseed rape is a remediation plant in rotation systems. Compared to 2020, the grain Cd concentrations of traditional rice, low-Cd rice, and maize in 2021 decreased by 73.8%, 65.7%, and 24.0% (below the safety limits), respectively. However, soybean increased by 71.4%. The LRO system featured the highest oil content of rapeseed (about 50%) and economic output/input ratio (1.34). Removal efficiency of total Cd in soil was 10.03% (TRO) > 8.3% (LRO) > 5.32% (SO) > 3.21% (MO). Crop uptake of Cd was influenced by bioavailability of soil Cd, and soil environmental factors regulated the bioavailable Cd. Redundancy analysis (RDA) indicated that soil nitrate‑nitrogen (NO3−-N) had a dominant impact on bioavailable Cd in soil, with variance contributions of 56.7% for paddy-upland (TRO and LRO) and 53.5% for dryland (MO and SO) rotation systems. The difference reflected that ammonium N (NH4+-N) was a secondary factor in paddy-upland rotations, while it was the available phosphorus (P) in dryland rotations, with variance contributions of 10.4% and 24.3%, respectively. The comprehensive evaluation of crop safety, production, economic benefits, and remediation efficiency revealed that the LRO system was efficient and more acceptable to local farmers, providing a new direction for the utilization and remediation of Cd-contaminated farmland.

FORMATION OF PRODUCTIVITY OF WINTER RAPE HYBRIDS DEPENDING ON ROW SPACING

FORMATION OF PRODUCTIVITY OF WINTER RAPE HYBRIDS DEPENDING ON ROW SPACING

Effects of Heat Stress during Seed Filling Stage on Brassica napus Seed Oil Accumulation and Chlorophyll Fluorescence Characteristics

As global temperature rise, the threat of heat stress to rapeseed production is becoming more obvious. Exploring the response characteristics of two important biological pathways, oil accumulation and photosynthesis, to heat stress during B. napus seed filling is helpful in the genetic improvement of heat-tolerant rapeseed. The effects of heat stress on seed oil accumulation and chlorophyll fluorescence characteristics of 29 B. napus germplasms with different oil content and environmental sensitivity, including 6 rapeseed varieties which exhibited environment-sensitive/insensitive and with high, medium or low oil content, were tested by whole plant heat stress or the in vitro silique culture system. Both assay exhibited similar trend on oil content of the rapeseed germplasms. The heat effect on the chlorophyll fluorescence kinetic parameters Fv/Fm, ETR and Y(II) were also consistent. Heat stress significantly decreased oil content, although there was abundant genetic variation on heat tolerance among the genotypes. Correlation analysis showed that the decrease rate of Fv/Fm of silique heat-stressed B. napus developing seed was positive correlative to the decrease rate of mature seed oil content of the whole plant heat-stressed rapeseed (R = 0.9214, P-value < 0.01). Overall, the results indicated that heat stress inhibited oil accumulation and photosynthesis in B. napus developing seed. The decrease rate of chlorophyll fluorescence parameter Fv/Fm of heat-stressed developing seed could be used as the index of heat tolerant rapeseed identification. Further, two heat insensitive rapeseed varieties with high oil content were identified.

Масличность рапса : ботаническая природа , биохимические особенности и пищевой потенциал

The annual rapeseed (Brassica napus L.), a member of the Brassicaceae family, has long been used as a source of technical oil. The development of low-erucic varieties (canola, less than 5% erucic acid) by Canadian breeders in the 1970s made rapeseed a valuable and promising oilseed crop. In the Russian Federation, rapeseed oil, extracted from the seeds of the mature pods of the plant, currently ranks third after sunflower and soybean oil in terms of production volume, surpassing them in a number of biochemical parameters: optimal ratio of ω‑6: ω‑3 fatty acids (1:3–2:1), high content of oleic acid (up to 79.57%), tocopherols (45–75 mg%), carotenoids (0.30–0.57 mg%) and sterols (0.5–1.0%). The high nutritional potential of rapeseed is explained by the availabilityof winter and spring forms, domestic and foreign varieties and hybrids, successful breeding for increased oil content and resistance to diseases, good yields in many natural zones of Russia, profitability of 100–150%, sometimes up to 400% and more, despite the need for strict adherence to cultivation technology, etc. In the fatty oil industry, oil yield refers to the content of crude fat and accompanying fat-like substances that pass from the seeds into the ether extract along with the fat. The cells of oil rapeseeds contain structures, that accumulate free lipids stored by the plant for use by the growing seedling: oleosomes and possibly fat inclusions in the cytoplasm, and plastoglobules in the plastids. The formation of neutral fats is a universal mechanism for “switching off” excess primary synthesis products from plant metabolism. The qualitative fatty acid composition of seed oils of different plant species (in contrast to oils extracted from the nonseed parts of the fleshy oil fruits) is quite similar; the amount of fatty acids and the fat-soluble components extracted with them (antioxidants, vitamins, etc.) is variable. Knowledge of the botanical nature and biochemical characteristics of the oil content of rapeseed will make it possible to obtain maximum nutritional benefits through breeding, agronomic and technological methods.

Simultaneous Extraction of Rapeseed Oil and Enzymatic Transesterification with Butanol in the Mineral Diesel Medium

Increasing environmental pollution is driving an increase in the production and use of biofuels. The cost price of biodiesel could be reduced by using low-quality oilseeds unfit for human consumption and by applying the simultaneous oil extraction and transesterification process, avoiding the oil pressure stage. The purpose of this study was to investigate the enzymatic biofuel production process (in situ) by using rapeseed with high oil acidity for simultaneous oil extraction and transesterification with a mixture of butanol and mineral diesel fuel. The investigation of the in situ process was performed using a mixture of butanol and mineral diesel and the most effective biocatalyst Lipozyme TL IM was selected. The novelty of this paper consists of the fact that mineral diesel was used as the oil extractant, and the amount chosen was such that, at the end, a mixture of fuel with a ratio 9:1 of mineral diesel to biodiesel was be produced. The experiments were carried out using ground rapeseeds under laboratory conditions. The efficiency of oil extraction was investigated by the FTIR spectrometry method, and the efficiency of transesterification was determined by the gas chromatography method. It was found that the optimal reaction duration was 7 h, reaction temperature was 40 °C, and lipase content was 6% (from the oil content in rapeseed). An oil extraction efficiency of 99.92 ± 0.04 (w/w) was observed at these conditions. A transesterification degree of 99.08 ± 0.08% (w/w) met with the requirements of the standards for biodiesel fuel. The physical and chemical properties of the produced fuel mixture met the requirements of the standards for mineral diesel and biodiesel; therefore, it can be used in diesel engines.

Variability of Essential and Nonessential Fatty Acids of Irish Rapeseed Oils as an Indicator of Nutritional Quality.

The low saturated fatty acid content of rapeseed oil has resulted in it being classed as one of the most health-benefiting culinary oils. This study determines whether Irish rapeseed oils contain identical fatty acid profiles or whether distinct profiles exist between producers and producers' successive oil batches. The fatty acid content of Irish rapeseed oils was determined in terms of the desirable MUFA and PUFA and saturated content of these oils. The fatty acid composition demonstrated significant differences in individual unsaturated fatty acid content, while total saturation had insignificant differences. Saturated fatty acid content ranged from 6.10 to 15.8%, while unsaturated fatty acids ranged from 84.20 to 90.10%. Moreover, individual fatty acid content exhibited significant differences (p < 0.05). Oleic acid (C18:1), linoleic acid (C18:2), and stearic acid (C18:0) contents were considered significantly different from other fatty acids detected. The third successive batch from each producer exhibited lower oleic acid content, and the third batch contained higher linoleic acid content, at the same time maintaining a desirable unsaturated fatty acid composition. Studies suggest that differences in the fatty acid composition may be due to cultivation practices such as climate, soil composition, sowing and harvesting, processing techniques, and oxidation reactions.

Investigation of the Performances of a Diesel Engine Operating on Blended and Emulsified Biofuels from Rapeseed Oil

The article discusses the possibility of using blended biofuels from rapeseed oil (RO) as fuel for a diesel engine. RO blended diesel fuel (DF) and emulsified multicomponent biofuels have been investigated. Fuel physicochemical properties have been analyzed. Experimental tests of a diesel engine D-245 in the operating conditions of the external characteristic curve and the 13-mode test cycle have been conducted to investigate the effect of these fuels on engine performances. CFD simulations of the nozzle inner flow were performed for DF and ethanol-emulsified RO. The possibility of a significant improvement in brake thermal efficiency of the engine has been noted. The efficiency of using blended biofuels from RO as a motor fuel for diesel engines has been evaluated based on the experimental test results. It was shown that in comparison with the presence of RO in emulsified multicomponent biofuel, the presence of water has a more significant effect on NOx emission reduction. The content of RO and the content of water in the investigated emulsified fuels have a comparable influence on exhaust smoke reduction. Nozzle inner flow simulations show that the emulsification of RO changes its flow behaviors and cavitation regime.

Эффективность способов основной обработки серой лесной почвы в звене севооборота при производстве семян яровых рапса и сурепицы

The article offers studies to determine the effectiveness of processing methods for the productivity of spring rapeseed and surepitsa on the dark gray forest soil of the Ryazan region. As a result, it was revealed that the maximum intensity of decomposition of flax tissue was recorded in the steam link of the crop rotation using the dump method of basic tillage for oilseed cabbage crops. The maximum yield of rapeseed was observed on the variant of the steam link of crop rotation + dump processing (31.3 c / ha), in the surepitsa (25.6 c/ha). A high increase in oilseeds in this variant is 4.7 c / ha, where the NSR05 factor with 0.97 c / ha relative to the minimum basic tillage was recorded in 2015. In the experiment, the trends of increasing the yield of oilseeds of spring rapeseed and surepitsa in the fallow link of the crop rotation relative to the row crop are revealed. The maximum oil content of rapeseed was noted in the rowed link of crop rotation during milling tillage (47.6%); in surepitsa – in the rowed link of crop rotation also during milling (45.1%). Keywords: SPRING RAPESEED, SPRING SUREPITSA, DARK GRAY FOREST SOIL, TILLAGE, YIELD, OIL CONTENT

Ultrastructural studies of seed coat and cotyledon during rapeseed maturation

Brassica napus L. (B. napus) is an important oil crop worldwide and it rapidly accumulates oil at late stage of seed maturation. However, little is known about the cellular mechanism of oil accumulation and seed color changes during the late stage of rapeseed development. Here, we analyzed the ultrastructure of seed coat, aleurone and cotyledon in embryos of B. napus from 25 to 70 days after flowering (DAF). The pigments, which were deposited on the cell wall of palisade cells in seed coat, determined dark black color of rapeseed. The chloroplasts degenerated into non-photosynthetic plastids which caused the green cotyledon to turn into yellow. The chloroplasts in aleurone and cotyledon cells respectively degenerated into remnants without inner and outer envelope membranes and ecoplasts with intact inner and outer envelope membranes. From 40 to 70 DAF, there were degraded chloroplasts without thylakoid, oil bodies contacting with plastids or protein bodies, big starch deposits of chloroplasts degrading into small particles then disappearing, and small endoplasmic reticulum (ER) in aleurone and cotyledon cells. Additionally, there were decreases of chlorophyll content and dramatic increases of oil content in rapeseed. These results suggested that the rapid oil accumulation was independent on the NADPH synthesized by photosynthesis of chloroplasts and probably utilized other sources of reductant, such as the oxidative pentose phosphate pathway during the late stage of rapeseed development. The triacylglycerol assembly presumably utilizes the enzymes in the plastid, cytosol or oil body of cotyledon and aleurone cells.

The blend fuel composition optimization to apply in tractor diesel engines

Introduction (problem statement and relevance).Today, vegetable oils, in particular, rapeseed oil (RO) are widely used types of fuels for diesel engines. The main physicochemical properties of RO are somewhat similar to diesel fuel (DF). However, one can highlight a large fraction of the oxygen content in it, which affects the fuel combustion intensity in diesel cylinders. In this regard, the addition of rapeseed oil is very important to optimize the composition of mixed fuel (MF) for its use in diesel engines. The purpose of the studywas optimizing the MF composition and obtaining experimental data of diesel engine effective performance by means of regression analysis.Methodology and research methods.To optimize the MF composition studies were carried out to determine the relative fractions of carbon, hydrogen, oxygen in RO and MF, as well as bench tests of diesel fuel and MF with various RO additives operation, followed by the regression analysis of effective indicators.Scientifi c novelty and results.The effective performance dependences of the diesel engine on the RO content in the MF have been determined. Basing on the obtained load characteristics of the diesel engine, it was concluded that an increase in the average effective pressure from 0.2 to 1.2 MPa, as well as in the share of RO in MF from 0 to 80%, would lead to an increase in the effective specifi c fuel consumption to 383–506 g/kW·h and the decrease in effective effi ciency by 14–28%. On the basis of the regression analysis the maximum values of the optimality criterion indicators D-optimum = 0.98–1.0 with the addition of RO to MF from 45 to 50% were determined.Practical signifi cance. The value of the maximum permissible composition of MF, consisting of 50–55% of diesel fuel and 45–50% of RO and ensuring maximum compliance with the specifi ed conditions of optimality on the diesel engine under consideration has been obtained.

Regional association analysis coupled with transcriptome analyses reveal candidate genes affecting seed oil accumulation in Brassica napus.

Regional association analysis of 50 re-sequenced Chinese semi-winter rapeseed accessions in combination with co-expression analysis reveal candidate genes affecting oil accumulation in Brassica napus. One of the breeding goals in rapeseed production is to enhance the seed oil content to cater to the increased demand for vegetable oils due to a growing global population. To investigate the genetic basis of variation in seed oil content, we used 60K Brassica Infinium SNP array along with phenotype data of 203 Chinese semi-winter rapeseed accessions to perform a genome-wide analysis of haplotype blocks associated with the oil content. Nine haplotype regions harbouring lipid synthesis/transport-, carbohydrate metabolism- and photosynthesis-related genes were identified as significantly associated with the oil content and were mapped to chromosomes A02, A04, A05, A07, C03, C04, C05, C08 and C09, respectively. Regional association analysis of 50 re-sequenced Chinese semi-winter rapeseed accessions combined with transcriptome datasets from 13 accessions was further performed on these nine haplotype regions. This revealed natural variation in the BnTGD3-A02 and BnSSE1-A05 gene regions correlated with the phenotypic variation of the oil content within the A02 and A04 chromosome haplotype regions, respectively. Moreover, co-expression network analysis revealed that BnTGD3-A02 and BnSSE1-A05 were directly linked with fatty acid beta-oxidation-related gene BnKAT2-C04, thus forming a molecular network involved in the potential regulation of seed oil accumulation. The results of this study could be used to combine favourable haplotype alleles for further improvement of the seed oil content in rapeseed.

Substantiation of methods for determination of the effectiveness of the use of alternative fuels in automotive diesel engines

The subject of the research is to determine the parameters of the operating cycle of a tractor diesel engine when it is running on a mixed fuel, consisting of diesel fuel and rapeseed oil. The main goal of this work is to determine the efficiency of using alternative fuels in automotive diesel engines. In order to determine the dependences of the parameters of the operating cycle, the theoretical and experimental studies of a tractor diesel engine were carried out on the following compositions of mixed fuel: with a rapeseed oil content of 20%, 55% and 80%, as well as pure diesel fuel. As a result of the research, the following was obtained: a nomogram for determining the value of the decrease in the ratio of the indicator efficiency to the excess air ratio, the dependence of the ratio of the indicator efficiency to the excess air ratio on the excess air ratio for different compositions of mixed fuel, diesel engine crankshaft rotational speed, load at 1800 min-1 and 1400 min-1. It was experimentally established that an increase in the proportion of rapeseed oil in mixed fuel from 0 to 80%, the speed from 1400 to 2000 min-1 and the excess air ratio from 1.18 ... 1.22 to 4.32 ... 5.61 led to a decrease in the indicator efficiency from 0.46 to 0.35, and the ratio decreased from 0.30 to 0.08, an increase in the load from 0.2 to 1.2 MPa, at 1800 min-1, and 1400 min-1, and the excess air ratio from 1.17 ... 1.68 to 3.53 ... 3.60 led to a decrease in the indicator efficiency from 0.44 ... 0.40 to 0.28 ... 0.22, and the ratio from 0.34 ... 0.25 to 0 , 10 0.09, respectively. A 4.5% reduction in the ratio is theoretically calculated when the diesel engine runs on a mixed fuel containing 80% rapeseed oil as compared to pure diesel fuel, and this reduction by 4.1% has been experimentally confirmed. The convergence of the calculated value of the decrease in the ratio with the experimental data was 91.11%. In this regard, the well-known statement about the equality of the input of heat with fuel requires clarification.

Mesophilic and Thermophilic Anaerobic Digestion of Model Kitchen Waste with Variation of Fat Content

AbstractAnaerobic digestion of model kitchen waste (MKW) produced on basis of real kitchen waste (KW) is studied in batch tests. The impact of fat content of three MKWs (14.7 %, 23 %, and 27.2 % rapeseed oil content) on the biogas production at mesophilic and thermophilic conditions and two loadings is examined. The loading of 5 gVSL−1 leads to fast degradation under both temperature modes and the volume of biogas produced is only weakly correlated to the oil content. For the loading of 25 gVSL−1 a strong retardation of biogas production, high concentration of volatile fatty acids and no correlation of oil content is observed.

The combination of palm and rapeseed oils emerges as a good dietary alternative for optimal growth and balanced lipid accumulation in juvenile gilthead sea bream reared at an elevated temperature

The aquaculture sector needs to develop new tools to optimally increase production for feeding, while facing future environmental changing conditions. One of the major improvements in this industry has been reducing the usage of fishmeal and fish oil (FO) in diet formulations to promote a more sustainable production. In this line and considering global warming, in the present study, juvenile gilthead sea bream were fed a diet with a 60% of FO substitution by vegetable oils (VO) containing mainly palm oil (P) (80% of the VO content) and maintained at 21 or 28 °C. Furthermore, in the condition of elevated temperature, fish were fed with two other diets with the same level of FO substitution and containing either 80% of the VO content of rapeseed oil (R) or an equilibrated combination of both VO (named PR). High temperature induced a significant increase in growth, but a reduction in the hepatosomatic and viscerosomatic indexes, and circulating triglycerides. Otherwise, significant differences were not found among the fish fed the three distinct diets at 28 °C for any of the somatic or plasma parameters analyzed. In the adipose tissue, the higher number of adipocytes of smaller size in fish maintained at 28 °C compared to fish at 21 °C, suggested an activation of adipogenesis despite the gene expression of peroxisome proliferator-activated receptor gamma (pparg) was significantly decreased. Furthermore, lipid droplets of smaller area and downregulation of the fat translocase cd36 and the fatty acid binding protein fabp1 were found in the liver of fish reared at 28 °C compared to those at 21 °C. Concerning differences among dietary groups, fish fed R diet showed in the liver a more extensive surface area occupied by lipid droplets of bigger size, while contrarily, fish fed PR diet had the smallest area filled with smaller lipid droplets. In adipose tissue, PR diet-fed fish had also adipocytes within the smaller size range. These histological data, together with the significant upregulation of several transcription factors (i.e. ppars) and genes related to lipid transport in the two tissues, indicated that the PR diet could be the most equilibrated for gilthead sea bream, to grow at a high rearing temperature while avoiding excessive fat accumulation. Thus, this study highlights the need of fine-tuning the substitution of FO by VO in the elaboration of feeds for this species to assure proper growth and physiological status in a future global warming scenario.

Preparation of rapeseed oil with superhigh canolol content and superior quality characteristics by steam explosion pretreatment technology.

In this study, rapeseed was pretreated by steam explosion pretreatment technology and subsequently pressed to prepare rapeseed oil. GC, UPLC, and HPLC techniques were employed to analyze the quality characteristics of the rapeseed oil, including the canolol content and other quality characteristics. Additionally, the effect of steam explosion pretreatment technology on the canolol content of rapeseed oil was studied and the formation mechanism of canolol elucidated. The results revealed that when the steam explosion pressure reached 1.0 MPa, the canolol content of the tested oil increased from 41.21 to 2,168.69 mg/kg (52.63‐fold increase) and that sinapic acid played a significant role in the conversion of canolol. Thus, the sinapine was converted into the intermediate (sinapic acid) by hydrolysis, which in turn was transformed into canolol through decarboxylation. The instantaneous high‐energy environment generated by steam explosion pretreatment could intensify the hydrolysis and decarboxylation reactions of sinapine and sinapinic acid, thereby significantly increasing the canolol content of the oil. To prove the superiority of steam explosion pretreatment, we compared it with other pretreatment technologies, including traditional high‐temperature roasting and popular microwave pretreatment. The results revealed that rapeseed oil prepared by steam explosion pretreatment displayed the best quality characteristics. This study can be a reference for the preparation process of rapeseed oil with superhigh canolol content and superior quality characteristics using steam explosion pretreatment.

Genome-wide Association Study on Seed Oil Content in Rapeseed and Con-struction of Integration System for Oil Content Loci

Genome-wide Association Study on Seed Oil Content in Rapeseed and Con-struction of Integration System for Oil Content Loci