An experiment was conducted to assess the effect of pre-sowing seed treatment with triacontanol (TRIA) in canola (Brassica napus L.) cultivar (RBN-3060) under saline stress. Canola seeds were soaked in three levels of TRIA (0, 0.5, and 1 mg L−1) for 12 hours. Three levels of salt stress (0, 100, and 150 mM NaCl) in full strength Hoagland's nutrient solution were applied to 56-days-old plants. Salt stress caused a significant reduction in growth, gas exchange, photochemical quenching (qP), and shoot and root K+ contents, while increased leaf glycine betaine, free proline, and shoot Na+ contents. Pre-sowing seed treatment with TRIA increased shoot fresh weight, number of seeds per plant, photosynthetic rate, transpiration rate, ratio of chlorophyll a/b, qP, electron transport rate, shoot and root K+ contents, and free proline and glycine betaine contents of canola plants at various TRIA levels under nonsaline or saline conditions.