Crop Contents Research Articles

Health Risk Assessment of Heavy Metals in Soil-crop System in Different Ecological Regions of Ningxia

Heavy metals are characterized by toxicity，non-biodegradability，and accumulation in the food chain；hence，o the distribution of heavy metals in farmland soils and crop food safety have attracted much attention. A total of 1 274 soil samples and crop samples were collected from farmland in Ningxia from 2017 to 2021 and bioconcentration coefficients were used based on the analysis of the elemental contents of Zn，Cr，Ni，Cu，Pb，As，Cd，and Hg. The ecological risk index and correlation analysis were used to characterize the heavy metal content and health risk assessment of the soil-crop system in Ningxia. The results showed that the soils in the study area were alkaline and the mechanical composition was dominated by pulverized and sandy grains，with a relatively small content of clay grains. The heavy metal contents in the soils were in the following order：Zn>Cr>Ni>Cu>Pb>As>Cd> Hg. Except for that of Cu and Ni，the contents of other heavy metals in the soils of the northern region were higher than those of the central and southern regions. The ecological risk index of soil heavy metals was Hg>Cd>As>Cu>Pb>Ni>Cr>Zn and the proportion of points with low and medium ecological risk index levels was high（98.58%），indicating that the soil environment was generally good. The average content of the eight heavy metals in crops did not exceed the limit value，and the average health risk index value was <1. However， the health risk index（HRI）of As in individual crops existed at points >1 in all three sub-regions and individual points with high values of HRI（HRI>1）for Cu and Ni were observed in crops in the northern region and for Pb in the north and south of the country，which require further attention. From the bioconcentration factor，crops showed no accumulation effect （BCF<1）for heavy metals such as As，Cd，Cr，Cu，Ni，Pb，and Zn and although the average uptake of Hg by crops did not show accumulation，a total of 31 crops in the three ecological zones showed some accumulation（BCF>1），which also requires further monitoring. Analysis using the Mantel's test showed that except for As and Ni in crops，which had a weak correlation with Cu content in the soil（0.05>Mantel's P>0.01），the other heavy metals in crops did not have a significant correlation with the corresponding soil heavy metals （Mantel's P≥0.05），indicating that the heavy metal contents in crops were less affected by soil heavy metals. RDA analysis showed that the sand content had a strong positive correlation with the transfer coefficients of heavy metals such as Hg，Ni，Pb，and Cd，while the powder content showed a strong negative correlation；however，the transfer coefficients of Cu，Zn，As，and Cr were not much affected by the mechanical composition of soils，and the correlation between the soil organic matter content and the transfer coefficients of heavy metals was also small. In summary，the quality of the soil environment and crops in the study area is generally good；however，attention must still be paid to the areas with high heavy metal content or exceeding the standard value to strictly control the input of pollutants，and at the same time，establishing long-term monitoring of the quality status of soil and agricultural products in the crop production area is a necessary control initiative.

Heavy Metal Pollution Characteristics and Risk Assessment of Soil-crop System in Gold Mine Activity Area

A contamination analysis was conducted on the soil and crops in the Liu Jia Dian gold mining area of the Pinggu District, Beijing, utilizing the contents and seven-step forms of heavy metals （Hg, Cd, Cr, Pb, and As） in soil and the heavy metal contents in crops at the same location. An assessment of ecological risk caused by heavy metal contamination in the soil and crops was carried out using the enrichment coefficient method and the risk assessment coding method. The study investigated the process of heavy metal absorption and enrichment in the soil-crop system, aiming to establish a scientific foundation for preventing and controlling soil pollution, as well as ensuring the safety of edible crops in the study area. Evidence indicated the presence of soil contamination by heavy metals in the study area, with Cd, Pb, and As being the primary pollutants. Moreover, the distribution of pollution levels could be categorized as severe, moderate, and mild, with proportions of 26.09%, 4.35%, and 10.87%, respectively. The content of each heavy metal decreased following the implementation of environmental governance； however, the ecological risk remains present. Hg, Cr, Pb, and As posed minor ecological risks； however, Cd had a high ecological risk, making it the primary soil ecological risk factor. Fruits, nuts, and cereals in the Pinggu gold mining area remained uncontaminated. Various degrees of contamination were found in melons, rhizomes, and leafy vegetables. The Chinese cabbage, Chinese chive, radish, and eggplant were heavily contaminated, with Cd and Pb being the primary sources of contamination. The accumulation of heavy metals in crops was intricately linked to gold mines and their mining operations. The accumulation of Cd in crops was mainly influenced by the high availability of Cd in soil, whereas the accumulation of Pb was mainly influenced by the high concentration of Pb in the soil. The concentration of heavy metals in the soil and their bioavailability in the Pinggu gold mining area indicated the accumulation of metal elements in crops and impacted the geographical distribution of heavy metal pollution in crops. The physiological characteristics of crops could affect the degree of heavy metal enrichment in the gold mining area. The order of heavy metal accumulation, from highest to lowest, was as follows： leafy vegetables, melons, rhizomes, cereal, fruits, and nuts. Peppers and eggplants were limited in their planting variations for melons, while leeks and cabbages were restricted for leafy vegetables, given the current agricultural planting conditions in the study area.

Variation in Chemical, Textural and Sensorial Traits Among Remontant Red Raspberry (Rubus idaeus L.) Cultivars Maintained in a Double-Cropping System

Remontant raspberry cultivars originally produce fruit in the upper part of primocanes in the fall, but if retained over winter, they can produce a second crop in the lower part of the floricanes the following spring. Maintaining remontant cultivars to yield twice during the cane’s growth cycle corresponds to a double-cropping system, which enables an increase in the total yield and the extension of the fruiting season. To date, there is little information on changes in fruit quality between primocane and floricane crops. The aim of this study was, therefore, to investigate variations in the content of sugars and organic acids, fruit weight, color and textural and sensorial attributes among five newly introduced remontant raspberry cultivars (‘Dafne’, ‘Kokanee’, ‘Paris’, ‘Versailles’ and ‘Primalba’) and the control cultivar ‘Enrosadira’. The specific aim was to evaluate how a double-cropping system in each cultivar may affect the variability in quality traits between primocane and floricane crops. The results showed a significant increase in fruit weight and individual and total sugar content in primocane crops, while significantly brighter red-colored and firmer fruits were observed in floricane crops. Cultivars did not differ from the control regarding total sugar content and sweetness index, while the content of individual sugars caused greater variations. The highest content of citric, malic and total acid (9.74, 1.42 and 11.25 mg 100 g−1 FW, respectively) were recorded in ‘Paris’, by which this cultivar was the only one distinguished from the control. ‘Dafne’ and ‘Versailles’ exhibited better internal and external fruit quality on primocanes, having significantly larger fruits (6.83 g and 6.37 g, respectively) and twice the increased sugar content. The lowest fruit weight was observed in ‘Kokanee’ for both primocane (4.63 g) and floricane (3.65 g) crops. ‘Kokanee’ and ‘Primalba’ also performed worse than the control for most sensory attributes in both seasons. Based on the analysis of the overall fruit quality linked to the appearance-, texture- and taste-related attributes that affect consumer preference, cultivars ‘Enrosadira’, ‘Versailles’ and ‘Dafne’ stood out, while ‘Paris’ showed high uniformity in fruit quality between crops, but scored the worst according to the total quality index.

Assessing Salinity, Drought and High Temperature Stress in Maize (Zea mays L.) and Wheat (Triticum aestivum L.) Varieties: Theoretical Combination as Multifactorial Stress

ABSTRACTMaize and wheat are two important cereal crops for the food security of the world population. However, constant climate change and the intensification of anthropic activities have intensified the emergence of stressful environmental in the various agricultural production systems around the world. Therefore, in this study we evaluate the chlorophyll content, photosynthesis, transpiration and grain yield of maize and wheat crops exposed to soil salinity, drought and high temperatures and determine the damage intensity of these stressing conditions and the theoretical multifactorial damage intensity. Field experiments were conducted during the 2022 and 2023 agricultural seasons in the Yaqui Valley, Sonora, Mexico. The treatments consisted of the cultivation of maize and wheat in three stressful production environments (soil salinity, drought and high temperatures) and a non‐stressful production environment (Control), with four repetitions. The tolerance and intensity index of abiotic stresses, as well as the intensity of theoretical multifactorial stress (salinity, drought and high temperatures), for morphological traits and grain yield, were calculated. The results reported that physiological traits and yield of maize and wheat are severely affected by drought stress conditions. High temperatures are the second abiotic stress factor that most limits physiological traits and grain yield of maize and wheat crops, being more harmful than soil salinity. The theoretical multifactorial stress has a greater negative impact on the yield of the elite maize and wheat varieties. The sum of a stressful environmental factor increases the intensity of multifactorial stress on grain yield of both cereal crops, especially for maize crop.

Long-Term Effects of Liming Sod-Podzolic Soil by Dolomite on the Microelement Composition of Plants &lt;i&gt;Hordeum&lt;/i&gt; L.

In a long-term precision microfield experiment, the effect of liming acidic sod-podzolic light loamy soil with dolorous flour (DF, dose range 0–2.0 Ha, 9th year of the aftereffect) on the soil reaction, the content of mobile compounds Zn, Cu, Mn, Fe, Cd, Pb in the soil, and the trace element composition of barley plants (Hordeum L.) was studied. It was revealed that the most sensitive to changes in the acid-base properties of the soil were Zn and Mn, the content of which in the organs of barley plants significantly decreased linearly in the dose range of DF 0–2.0 Ha. The consequence was significant even at the minimum dose of DF (0.2 Ha). There was a tendency to a decrease in the Fe content in straw and ear of plants (r = –0.572 and –0.570, respectively) in the indicated dose range. The data obtained confirmed that precipitation can lead to an increase in the Cd content in grain crops: in the dose range of DF 0.8–2.0 Ha, the increase in the element content in the ears of plants relative to the control variant of the experiment reached 1.6–2.0 times, and in straw increased linearly in the dose range of DM 0–1.0 Ha (r = 0.945), the content of Cu and Pb in barley plants was weakly dependent on the dose of liming agent.

The Influence of Cultivation, Storage and Processing Technology on the Nitrate Content in Potato Tubers and Vegetable Crops as the Example of Ecologically and Hygienically Oriented Organic Agricultural Nature Management

The article presents the results of studies of the influence of organic and mineral fertilizers, as well as storage and processing technologies on the level of nitrates in potatoes, carrots and cabbage. The study demonstrated that in order to obtain the high yield of environmentally friendly vegetable crops, it is necessary to use biohumus at a norm of 8 t/ha or the combination of 6 t/ha biohumus and N35P35K40. This treatment can provide the potato yield of 420-440 c/ha, carrots 223-240 c/ha, cabbage 500-520 c/ha, while not exceeding the maximum allowable concentration (MAC) of nitrates. At a same time, studies have revealed that storage and processing of these vegetables under the required standard conditions can significantly reduce the amount of nitrates contained in the products. The work we have done shows an example of organic farming as the ecological and climatic project for carbon sequestration in soils, bioimprovement and bioprotection of soils, for increasing the yield and hygienic quality of the harvest. When processing the resulting vegetable products, toxic hazards to public health are eliminated and the ecological safety of the environment is ensured. Additional measures are needed to control the application of fertilizers to soils, the quality of agricultural products during collection, storage and processing.

Mitigating Mercury Accumulation and Enhancing Methylmercury Degradation in Rice: Insights from Zinc-Mercury Antagonism at Molecular Levels.

Zn as an essential element has the potential to protect against mercury (Hg) toxicity in rice. However, the antagonistic effects between Zn and Hg in rice and their mechanisms remain unknown. This study proposed a promising strategy for Zn application to mitigate Hg accumulation and toxicity in rice and revealed the underlying molecular mechanisms. The findings revealed that Zn supplementation significantly reduced the uptake and transportation of both IHg and MeHg in rice, thereby alleviating Hg phytotoxicity. In particular, Zn profoundly mitigated Hg-induced oxidative damage to rice, which was attributed to the redistribution of Hg and Zn in the root and Zn competing for binding sites on glutathione. The co-binding of Zn2+ and HgCH3+ within the same active sites of Zn transporters can promote the transfer of regions with a high charge density distribution at the highest occupied molecular orbital (HOMO) level. This process facilitates proton attack on the Hg-C bond, thereby enhancing MeHg demethylation in rice. By elucidating the molecular mechanisms of Zn, IHg, and MeHg interactions in rice, this study offers new insights for developing efficient strategies to mitigate Hg risks while boosting the Zn content in crops, thereby fortifying food safety.

Diverse Farming Systems and their Impact on Macro and Microelement Content of Vegetables & Crops.

The present study investigates the effect of conventional and organic farming systems on the nutritional profile of crops. Different crops, namely -millet, sorghum, sesame, mustard, fenugreek, berseem, pea, potato, and onion were cultivated through conventional agriculture in which chemical fertilizers like urea, DAP (Diammonium Phosphate) and pesticides were used and organic farming in which organic fertilizers like seaweed and vermicompost were used. The experimental study was done on a field in north India from 2019 to 2021 in six different seasons, and the nutrient profile of the crops with respect to macroelements (S, K, Na, P, Ca, Mg) and microelements (B, Cu, Fe, Mn, Zn, Al) was compared. Macro and microelements were analyzed by Element analyzer and ICP-OES in both types of farming systems. The content of macro, as well as microelements, was found to be significantly higher in all the organically produced crops as compared to the conventionally grown crops. Significant differences were observed in the macroelement content of organic onion (P- 900 mg/kg, K-2000mg/kg) and organic pea (K 2250 mg/kg) as compared to the content of conventionally grown onion (P-756 mg/kg, K- 1550 mg/kg) and pea (K-2000 mg/kg). Similarly, microelement content in the organic sesame (Fe - 3.12 mg/kg), organic millet (Fe- 2.19 mg/kg), and organic potato (Zn-200 mg/kg) was higher as compared to conventionally grown sesame (Fe 2.05 mg/kg), millet (Fe- 1.56 mg/kg) and potato (Zn 167 mg/kg). This investigation concludes that crops with optimum nutritional content can be produced through organic farming with minimum input and maximum production.

Saga of Soggy Sauerkraut

The creation of undesirable (soggy) sauerkraut resulted in the loss of $1,000,000 worth of organic sauerkraut in 2022, which prompted a multistep investigation of the cause and potential solution. The cause of this condition has been previously reported as unique fermentation conditions and the lack of key trace nutrients essential for cabbage (Brassica oleracea var. capitata) cell wall integrity. Because the condition was limited to organic sauerkraut in 2022, this investigation initially focused on differences in fermentation conditions between organic and conventional sauerkraut. No differences in fermentation conditions accounted for the condition; therefore, attention was focused on analyzing the mineral content of cabbage grown for sauerkraut production that pinpointed a deficiency in critical micronutrients such as iron, copper, manganese, boron, and zinc. This deficiency was traced to the use of poultry manure that was contaminated with glyphosate residue from conventionally fed turkeys and chickens that consumed genetically engineered (GE) feed and used as the fertilizer for organic cabbage production. The presence of glyphosate, a potent mineral chelator and antibiotic, was identified as a significant factor that impairs the absorption and physiological function of essential minerals in the shikimate metabolic pathway whereby cell walls and lignin are produced, thus compromising the structural quality of the sauerkraut. After this discovery, the study progressed to evaluate various remediation strategies aimed at eliminating glyphosate from the soil and restoring nutrient uptake. Corn grain and silage were selected as the test crops for this phase. Among the tested remediation solutions were raw sauerkraut juice containing Lactobacillus plantarum, which is reported to degrade glyphosate in the rumen of dairy cows and two patented proprietary microbial mixtures, PB027 and PB027SK, that degrade glyphosate by all three of the known metabolic pathways. These treatments were specifically formulated to degrade residual glyphosate in the soil. The results showed that these interventions could reduce soil glyphosate levels by 80% to 90% within 6 to 7 months to significantly enhance both the yield and quality of corn grain and silage. The increase in corn grain yield from glyphosate degradation on the Shiocton silt loam soil was 907.89 kg·ha−1 (13.5 bushels/acre). The increase in yield on the irrigated Kidder sandy loam soil was quantified at 726.31 kg·ha−1 (10.8 bushels/acre) for corn grain and 6.62 t·ha−1 (2.68 t/acre) for silage, with an additional improvement in silage feed quality beneficial for milk production. The findings underscore the importance of addressing both micronutrient sufficiency and glyphosate residue in soil to ensure the optimal growth of cabbage and the quality of sauerkraut produced. By successfully identifying manure as a subtle source of nutrient immobilization and implementing effective soil remediation techniques, this research highlights a clear path forward for improving crop yield and quality to ultimately enhance the structural integrity and consumer acceptance of sauerkraut. This study has broader applications for the nutritional content and crop yields of many organic crops that use conventional poultry and animal manures that may contain glyphosate in desiccated plant tissues or GE feeding operations.

Assessment of Potentially Toxic Elements and Their Risks in Water and Sediments of Kitengure Stream, Buhweju Plateau, Uganda

Artisanal and small-scale gold mining (ASCGM) provides a livelihood for many communities worldwide, but it has profound environmental impacts, especially on the quality of nearby water resources. This study assessed the impacts of ASCGM on the physicochemical quality of water and sediments from Kitengure stream, Buhweju Plateau, Western Uganda. Surface water (n = 94) and superficial sediments (n = 36) were sampled between October 2021 and January 2022 from three different sections of Kitengure stream (upstream, midstream around the ASCGM area, and downstream). The samples were analyzed for various physicochemical parameters and selected potentially toxic elements (PTXEs), namely: zinc (Zn), cadmium (Cd), lead (Pb), copper (Cu), and arsenic (As). A health risk assessment was performed using the hazard index and incremental life cancer risk methods. Pearson’s bivariate correlation, geoaccumulation, and pollution indices were used to establish the sources and potential risks that PTXEs in sediments could pose to aquatic organisms. The results indicated that water in Kitengure stream draining the ASCGM site was highly colored (1230.00 ± 134.09 Pt-co units; range = 924.00–1576.00 Pt-co units) and turbid (194.75 ± 23.51 NTU; range = 148–257 NTU). Among the five analyzed PTXEs, only Cd (0.082 ± 0.200–0.092 ± 0.001 mg/L) and Cu (0.022 ± 0.004–0.058 ± 0.005 mg/L) were detected in water, and Cd was above the permissible limit of 0.003 mg/L for potable water. Upon calculating the water quality index (WQI), the water samples were categorized as very poor for upstream samples (WQI = 227) and unfit for use (WQI = 965 and 432) for midstream and downstream samples, respectively. In sediments, the mean concentration ranges of Zn, Cd, Pb, Cu, and As were 0.991 ± 0.038–1.161 ± 0.051, 0.121 ± 0.014–0.145 ± 0.025, 0.260 ± 0.027–0.770 ± 0.037, 0.107 ± 0.017–0.422 ± 0.056, and 0.022 ± 0.002–0.073 ± 0.003 mg/kg, respectively, and they were all below their average shale, toxicity reference, and consensus-based sediment quality guidelines. Geoaccumulation indices suggested that there was no enrichment of the elements in the sedimentary phase and the associated ecological risks were low. However, there were potential non-carcinogenic health risks that maybe experienced by children who drink water from Kitengure stream. No discernable health risks were likely due to dermal contact with water and sediments during dredging or panning activities. It is recommended that further studies should determine the total mercury content of water, sediments, and crops grown along the stream as well as the associated ecological and human health risks.

Utilizing VSWIR spectroscopy for macronutrient and micronutrient profiling in winter wheat.

This study explores the use of leaf-level visible-to-shortwave infrared (VSWIR) reflectance observations and partial least squares regression (PLSR) to predict foliar concentrations of macronutrients (nitrogen, phosphorus, potassium, calcium, magnesium, and sulfur), micronutrients (boron, copper, iron, manganese, zinc, molybdenum, aluminum, and sodium), and moisture content in winter wheat. A total of 360 fresh wheat leaf samples were collected from a wheat breeding population over two growing seasons. These leaf samples were used to collect VSWIR reflectance observations across a spectral range spanning 350 to 2,500 nm. These samples were then processed for nutrient composition to allow for the examination of the ability of reflectance to accurately model diverse chemical components in wheat foliage. Models for each nutrient were developed using a rigorous cross-validation methodology in conjunction with three distinct component selection methods to explore the trade-offs between model complexity and performance in the final models. We examined absolute minimum predicted residual error sum of squares (PRESS), backward iteration over PRESS, and Van der Voet's randomized t-test as component selection methods. In addition to contrasting component selection methods for each leaf trait, the importance of spectral regions through variable importance in projection scores was also examined. In general, the backward iteration method provided strong model performance while reducing model complexity relative to the other selection methods, yielding R 2 [relative percent difference (RPD), root mean squared error (RMSE)] values in the validation dataset of 0.84 (2.45, 6.91), 0.75 (1.97, 18.67), 0.78 (2.13, 16.49), 0.66 (1.71, 17.13), 0.68 (1.75, 14.51), 0.66 (1.72, 12.29), and 0.84 (2.46, 2.20) for nitrogen, calcium, magnesium, sulfur, iron, zinc, and moisture content on a wet basis, respectively. These model results demonstrate that VSWIR reflectance in combination with modern statistical modeling techniques provides a powerful high throughput method for the quantification of a wide range of foliar nutrient contents in wheat crops. This work has the potential to advance rapid, precise, and nondestructive field assessments of nutrient contents and deficiencies for precision agricultural management and to advance breeding program assessments.

Hydroponic Cultivation of Spices and Aromatics: Techniques, Benefits and Challenges

The global shift towards urbanization, with 55% of the population residing in urban areas and an anticipated rise to 68% by 2050, presents challenges to traditional farming. The increasing world population, further intensifies the strain on available agricultural land due to urban expansion. Climate change combines with these issues, make conventional cultivation more challenging. Alternative agricultural practices, such as hydroponics, gain significance in addressing these concerns. Aromatic and spices have been used for centuries for their medicinal and culinary properties. They contain various essential oils, flavonoids, and other phytochemicals responsible for their distinctive flavour and aroma, as well as their health benefits. The rising global demand for natural products from herbs and spices has led to its market growth. Hydroponics, a technology for cultivating plants in nutrient solutions with or without an artificial medium such as sand, gravel, vermiculite, rockwool, peat moss, coir or sawdust, offers a promising solution. This paper explores the potential of hydroponic cultivation for planting material production, enhancing the yield, nutritional composition, essential oil content and medicinal properties of various spice and aromatic crops, including black pepper, saffron, ginger, garlic, coriander, mint, celery, fenugreek, parsley, thyme, rosemary, green mustard, basil and others. The main challenges in hydroponic production of these crops include high initial investment, precise monitoring and lack of technical knowledge. Hence by reviewing recent literature, this paper aims to provide insights into the classification of hydroponics and its different techniques, benefits and challenges of hydroponic cultivation, various growing media and nutrient solutions used, types of hydroponics utilized and their purposes in the production of diverse herbs and spices. Embracing hydroponic techniques could contribute to sustainable agricultural practices, particularly in urbanized environments where traditional farming faces constraints.

Metabolic engineering of the serine/glycine network as a means to improve the nitrogen content of crops.

In plants, L-serine (Ser) biosynthesis occurs through various pathways and is highly dependent on the atmospheric CO2 concentration, especially in C3 species, due to the association of the Glycolate Pathway of Ser Biosynthesis (GPSB) with photorespiration. Characterization of a second plant Ser pathway, the Phosphorylated Pathway of Ser Biosynthesis (PPSB), revealed that it is at the crossroads of carbon, nitrogen, and sulphur metabolism. The PPSB comprises three sequential reactions catalysed by 3-phosphoglycerate dehydrogenase (PGDH), 3-phosphoSer aminotransferase (PSAT) and 3-phosphoSer phosphatase (PSP). PPSB was overexpressed in plants exhibiting two different modes of photosynthesis: Arabidopsis (C3 metabolism), and maize (C4 metabolism), under ambient (aCO2) and elevated (eCO2) CO2 growth conditions. Overexpression in Arabidopsis of the PGDH1 gene alone or PGDH1, PSAT1 and PSP1 in combination increased the Ser levels but also the essential amino acids threonine (aCO2), isoleucine, leucine, lysine, phenylalanine, threonine and methionine (eCO2) compared to the wild-type. These increases translated into higher protein levels. Likewise, starch levels were also increased in the PPSB-overexpressing lines. In maize, PPSB-deficient lines were obtained by targeting PSP1 using Cas9 endonuclease. We concluded that the expression of PPSB in maize male gametophyte is required for viable pollen development. Maize lines overexpressing the AtPGDH1 gene only displayed higher protein levels but not starch at both aCO2 and eCO2 conditions, this translated into a significant rise in the nitrogen/carbon ratio. These results suggest that metabolic engineering of PPSB in crops could enhance nitrogen content, particularly under upcoming eCO2 conditions where the activity of GPSB is limited.

Studies of genetic diversity and genome-wide association for vitamin C content in lettuce (Lactuca sativa L.) using high-throughput SNP arrays.

Lettuce (Lactuca sativa L.) is a source of beneficial compounds though they are generally present in low quantities. We used 40K Axiom and 9K Infinium SNP (single nucleotide polymorphism) arrays to (i) explore the genetic variability in 21 varieties and (ii) carry out genome-wide association studies (GWAS) of vitamin C content in21 varieties and a population of 205 plants from the richest variety in vitamin C ('Lechuga del Pirineo'). Structure and phylogenetic analyses showed that the group formed mainly by traditional varieties was the most diverse, whereas the red commercial varieties clustered together and very distinguishably apart from the rest. GWAS consistently detected, in a region of chromosome 2, several SNPs related to dehydroascorbic acid (a form of vitamin C) content using three different methods to assess population structure, subpopulation membership coefficients, multidimensional scaling, and principal component analysis. The latter detected the highest number of SNPs (17) and the most significantly associated, 12 of them showing a high linkage disequilibrium with the lead SNP. Among the 84 genes in the region, some have been reported to be related to vitamin C content or antioxidant status in other crops either directly, like those encoding long non-coding RNA, several F-box proteins, and a pectinesterase/pectinesterase inhibitor, or indirectly, like extensin-1-like protein and endoglucanase 2 genes. The involvement of other genes identified within the region in vitamin C levels needs to be further studied. Understanding the genetic control of such an important quality trait in lettuce becomes very relevant from a breeding perspective.

Nutrient Composition of Arugula Leafy Greens Following Application of Ascorbic Acid Foliar Sprays

Agronomic biofortification of vitamin C is a promising strategy to address vitamin C deficiencies in populations that lack access to diverse and nutritious diets. Different application methods can improve the vitamin C content in various crops; however, foliar application of ascorbic acid (AA) solutions has been under-explored. To determine if spray concentration, number of applications, and day of harvest would affect vitamin C in arugula leafy greens, foliar sprays consisting of 100 ppm and 200 ppm of AA and deionized (DI) water control were applied. Treatment application was initiated during the baby-leaf stage and subjected to a total of three sprays over the course of the experiment, followed by harvest at two days and four days after spraying (DAS). The harvested plants were measured for fresh and dry biomass and analyzed for vitamin C content, mineral composition, chlorophyll levels, and carotenoid content. The results of this study demonstrated a notably elevated total vitamin C concentration (p = 0.0002) and AA content (p = 0.02) in arugula leaves treated with a 200 ppm AA spray following the third application and harvested at 4 DAS. Additionally, the AA application improved the fresh and dry weight of leafy greens but did not exhibit any significant variances regarding the mineral composition of P, K, Ca, Mg, S, B, Zn, Mn, and Fe. Alternatively, AA foliar sprays reduced Cu content in leaves suggesting that AA reduced Cu accumulation in arugula leafy greens. In summary, the findings of this study establish that the foliar application of 200 ppm AA is an effective approach for increasing the vitamin C content in arugula leafy greens while improving the plant’s biomass, mineral composition, and stress responses. These biofortified arugula leafy greens exhibit the potential to offer plant protection against environmental stresses and a more consistent supply of vitamin C to humans upon consumption.

Biofortification: Future Challenges for a Newly Emerging Technology to Improve Nutrition Security Sustainably

Biofortification was coined as a term to define a plant breeding strategy to increase the micronutrient content of staple food crops to reduce the burden of micronutrient deficiencies in low- and middle-income countries. In 2003, the HarvestPlus program, based in the centers comprising the Consultative Group on International Agricultural Research, was initiated to implement the biofortification strategy. This article discusses what has been achieved, what has been learned, and the key challenges to embed biofortification in food systems and to expand its impact. Cost-effectiveness is key to the biofortification strategy. Biofortification piggybacks on the agronomically superior varieties being developed at agricultural research centers. Central plant breeding research discoveries can be spread globally. Farmers have every motivation to adopt the latest high-yielding, high profit crops. High productivity leads to lower food prices. As a consequence, consumers can increase their mineral and vitamin intakes at no additional cost by substituting biofortified staple foods 1-for-1 for nonbiofortified staple foods. After 20 y of investment, biofortified staple food crops are being produced by farmers in over 40 countries and are eaten by hundreds of millions of people. Published nutrition trials have shown nutrient-rich crops to be efficacious. The biofortification strategy is now recognized by the international nutrition community as one effective approach among several interventions needed to reduce micronutrient deficiencies. This is a promising beginning. However, biofortification is still a newly emerging technology. A limitation of biofortification as implemented to date is that densities of single nutrients have been increased in given staple food crops. To reach a higher trajectory, the impacts of biofortification can be multiplied several-fold using genetic engineering and other advanced crop development techniques to combine multiple-nutrient densities with climate-smart traits.

Improved Agronomic Practices on Enhancement of Quality Forage Production for Livestock Farming

The lack of nutrient-rich fodder and forage is one of the problems of livestock farming. Adequate tillage operations, timely and suitable water management, weed management, insect pests and disease management, fertilizer management, seeding at the proper time and seed rate, timely harvesting, and other agronomic techniques can all help to increase the nutritional content and yield of fodder and forage crops. A number of research and review papers have been systematically reviewed in this study. The use of tillage practices such as primary, secondary, conventional, and deep tillage enhances dry matter and yield of green fodder as compared to zero tillage. The organic matter (OM) content and dry matter (DM) of fodder crops are increased by regular and appropriate irrigation. The DMD (dry matter digestibility) and CP (crude protein) contents of early harvested forages are higher than those of late harvested forages. The application of nitrogen promotes crop development and growth, increasing the production of green fodder and improving its quality. Intercropping is essential for increasing the yield of fodder crops. Compared to sole cropping of maize and cowpea, the yield is found higher in intercropping of maize + cowpea. The production and quality of fodder are decreased by late seeding. The management of insect pests and diseases enhances the production and quality of fodder and forage. Thus, we draw the conclusion that fodder and forage crops production and their quality parameters are greatly influenced by agronomic practices.

Implications of soil waterlogging for crop quality: A meta-analysis

Soil waterlogging in many arable regions of the world challenge the quantum and quality of crop production. While previous studies have assessed the impact of waterlogging on crop yields, understanding of how waterlogging implicates with crop quality remains in its infancy. Here, we conduct a systematic literature review and meta-analysis to assess how waterlogging influences grain quality. We also explore the role of engineering and agronomic strategies for alleviating adverse effects of waterlogging. We reveal that soil waterlogging has less impacts on grain quality than on yield; the latter decreasing by an average of 23 %, while average grain protein and starch content of waterlogged crops reduced by 6.7 % and 7.3 %, respectively. We attribute these differences to underlying mechanics of yield and grain quality formation, as well as biological processes conferring adaptation, plasticity and recovery. Reduced grain quality under waterlogging is associated with decreased activity of enzymes involved in leaf nitrogen and carbon metabolism. Unlike yields however, grain quality suffers less deterioration with prolonged waterlogging, with ultimate effects realized being a function of species-specific tolerance, timing and duration of waterlogging relative to crop stage, soil type and growing season weather. We underscore the potential in engineering and/or agronomic interventions for alleviating detrimental effects of waterlogging.

Numerical investigation of future potato yields in Bangladesh using Machine learning and IoT

The changing environment has significantly affected Bangladesh for the past several decades, and a number of minerals and nutrients are in short supply in the human population. However, not only are we being affected by the effects of climate change, but also the crops cultivated on our farms are feeling its impact. The nutrient content and growth of food crops are affected by many components of climate change, such as rising temperatures, increased precipitation, rising CO2 levels, etc. It is commonly regarded that potatoes are one of the healthiest and most popular foods in Bangladesh, with an abundance of nutrients and electrolytes that are beneficial to the body. However, in the not-too-distant future, the production of potatoes will be affected by the high temperature and decreased precipitation resulting from climate change. As potatoes are one of the most essential staple foods in Bangladesh, a viable solution must be found for continuous potato production in the country. A comprehensive examination of the evolution, production, and important nutrients of potatoes will be performed in this study. A range of sensors, networks, drones, cameras, and applications will be collected for the purpose of monitoring the potato field and ensuring that the crops are cultivated in an acceptable environment. This idea can aid our country economically and agriculturally by increasing potato production. Potatoes are affordable for consumers from all socioeconomic classes. Through this idea, Producers will achieve a successful harvest of nutrient-rich potatoes for public consumption.

Carotenoid-carbohydrate crosstalk: evidence for genetic and physiological interactions in storage tissues across crop species.

Carotenoids play essential roles in photosynthesis, photoprotection, and human health. Efforts to increase carotenoid content in several staple crops have been successful through both conventional selection and genetic engineering methods. Interestingly, in some cases, altering carotenoid content has had unexpected effects on other aspects of plant metabolism, impacting traits like sugar content, dry matter percentage, fatty acid content, stress tolerance, and phytohormone concentrations. Studies across several diverse crop species have identified negative correlations between carotenoid and starch contents, as well as positive correlations between carotenoids and soluble sugars. Collectively, these reports suggest a metabolic interaction between carotenoids and carbohydrates. We synthesize evidence pointing to four hypothesized mechanisms: (1) direct competition for precursors; (2) physical interactions in plastids; (3) influences of sugar or apocarotenoid signaling networks; and (4) nonmechanistic population or statistical sources of correlations. Though the carotenoid biosynthesis pathway is well understood, the regulation and interactions of carotenoids, especially in nonphotosynthetic tissues, remain unclear. This topic represents an underexplored interplay between primary and secondary metabolism where further research is needed.