Objective: The study investigated the intersection between Industry 4.0 and biotechnology, with the aim of analyzing how these areas converge to promote innovation and sustainability in contemporary industry. Methodology: An integrative review approach was adopted, using several SciELO and Google Academic databases As an inclusion criterion, Brazilian articles were selected in Portuguese and published between 2020 and 2023. Results and data analysis: The results highlighted significant advances, such as advanced automation, artificial intelligence and 3D printing in Industry 4.0, that not only optimize industrial production, but also strengthen sustainable practices by reducing waste and improving the use of natural resources. Biotechnology has emerged as an essential pillar, offering solutions such as bioprinting and cellular therapies, expanding the possibilities for sustainable product development. Despite the benefits, ethical and regulatory challenges have been identified that require appropriate government policies and innovation strategies to ensure the ethical use of these technologies. Conclusion: In conclusion, the convergence between Industry 4.0 and biotechnology not only redefines modern industry standards, but also establishes a new sustainable innovation paradigm, contributing to a more responsible and competitive future globally.