Containment Of Species Research Articles

Facility design and management. Strategies for high level biocontainment.

UK Health Security Agency is required to investigate the pathogenesis of emerging or re-emerging infections and to test novel interventions, such as vaccines and therapeutics against these and other diseases, such as tuberculosis and Ebola, that have a significant impact on human health world-wide. Research into the causative agents (mainly BSL 3 and 4) using a wide range of animal species as pre-clinical models brings a number of challenges in terms of effective biocontainment to address human safety whilst optimising delivery of scientific objectives and the welfare of the animals. Here we describe the strategies used for high containment of species that include mice, ferrets, hamsters, rabbits and macaques that have been infected with high consequence pathogens. To ensure relevance of these models we frequently challenge by the aerosol route and monitor the development of disease and protective or therapeutic efficacy by methodologies similar to those used in the clinic. We have devised methods of sampling that can inform on pathogenesis and immune function that include lung lavage and medical imaging such as computed tomography and positron emission tomography-computed tomography. Imaging assists our assessment of progression to disease whilst providing refinement in application of early humane endpoints. We have developed directional flow containment systems that provide quantifiable operator protection whilst allowing group housing and a wide range of enrichment strategies appropriate for each species. Furthermore, we have demonstrated our improvements in animal welfare through use of a software-based Animal Welfare Assessment Grid that was developed with help of NC3Rs funding and enables us to quantify the lifetime experience of animals.

Darwin's and Wallace's Revolutionary Research Programme

Research programmes are sets of problems preferred on epistemic grounds and including preferred heuristics for inquiry. Charles Lyell's research programme for biogeograpy includes the problem of explaining the distribution of species constrained by laws governing locomotion and containment of species. Included in the programme are laws governing the supernatural introduction of replacement species. Wallace and Darwin derected arguments against the putative intelligibility of this aspect of Lyell's programme before discovering natural selection, and their defence, at this time of natural laws governing the introduction of new species is interesting as a defence of a noval programme of research, not a defence of a proposed theroy. These arguments are presented and an epistemological foundation is offered for them.