Containing Carboxymethyl Cellulose Research Articles

Sodium alginate/carboxymethylcellulose gel formulations containing Capparis sepieria plant extract for wound healing.

Aim: Using appropriate wound dressings is crucial when treating burn wounds to promote accelerated healing.Materials & methods: Sodium alginate (SA)-based gels containing Carboxymethyl cellulose (CMC) and Pluronic F127 were prepared. The formulations. SA/CMC/Carbopol and SA/CMC/PluronicF127 were loaded with aqueous root extract of Capparis sepiaria. The formulations were characterized using appropriate techniques.Results: The gels' viscosity was in the range of 676.33±121.76 to 20.00±9.78cP and in vitro whole blood kinetics showed their capability to induce a faster clotting rate. They also supported high cell viability of 80% with cellular migration and proliferation. Their antibacterial activity was significant against most bacteria strains used in the study.Conclusion: The gels' distinct features reveal their potential application as wound dressings for burn wounds.

Preparation and characterization of an antibacterial CMC/PCL hydrogel films containing CIP/Cur: In vitro and in vivo evaluation of wound healing activity

Wound healing process significantly impeded by prolonged inflammation responses, infection at the wound site, and insufficient angiogenesis. The sustained release of anti-inflammation and anti-bacterial drugs has the potential to control immune responses, improve angiogenesis, accelerate wound healing, and re-epithelialization. In this research a multifunctional hydrogel containing carboxy methyl cellulose (CMC) and polycaprolactone (PCL), along with Ciprofloxacin (CIP) and Curcumin (Cur) was developed. Physicochemical characteristics as well a biological one such as antibacterial and drug release properties, Collagen deposition, inflammatory responses, angiogenesis, and epidermal regenerated thickness were investigated via in vitro and in vivo assay. The results revealed that the CMC/PCL-based wound dressing has the potential to provide an appropriate level of water absorption (~300 %) to absorb wound exudate and ideal WVTR in range of 2279–2363 g/m2 for wound healing application. Addition of CIP and Cur to CMC/PCL hydrogel improved the skin cell proliferation, cell migration, and antibacterial activity. It also led to superior Collagen I synthesis (~2–4 times), controlled pro-inflammatory and improved anti-inflammatory cytokine secretion of macrophages, and accelerated wound healing in comparison to CMC/PCL hydrogel. In conclusion, the engineered multifunctional hydrogel showed a potential effect for accelerating wound healing and skin regeneration.

Improving the rheological properties of ultra-processed powdered goat milk beverage using texture additives.

This study aimed to investigate the static and dynamic rheological properties of an ultra-processed powdered goat milk beverage containing carboxymethyl cellulose (CMC), guar gum (GG), and xanthan gum (XG), using a mixture design. Fourteen samples were evaluated, in addition to the original beverage. The flow curves classified the fluids as non-Newtonian and characterized all the reconstituted beverages with pseudoplastic behavior, precisely due to the addition of hydrocolloids that increased the viscosity, and the fresh beverage sample showed the behavior of a dilatant fluid. The stress sweep verified the changes in the storage module (G') and the loss module (G″), the G' ranged from 1.97 to 20.36 (Pa) and the G″ ranged from 5.94 to 11.30 (Pa), demonstrating that there was the formation of beverages with elastic and viscous behaviors. The results showed a synergistic effect between the texture improvers, and the formulations with GG identified a greater effect on the tension. The frequency sweep tests showed that the behavior of the samples was that of a weak gel; however, when subjected to a certain frequency, the values of G' exceeded the value of G″, becoming a sample with more elastic characteristics and consequently, presenting the behavior of a stronger gel. Finally, according to the data presented, it is assumed that formulations containing GG and xanthan gum may produce desirable consumer properties for the reconstituted goat milk drink. Samples with a higher concentration of GG exhibited improved rheological performance, characterized by greater consistency, increased resistance to tension, and higher viscosity at low frequencies. PRACTICAL APPLICATION: The powdered goat milk drink is an innovative product that meets the demands of the food industry and consumers by offering a nutritious and sustainable beverage alternative.

Enhanced chemotaxis efficiency of Escherichia coli in viscoelastic solutions.

Bacteria inhabit complex environments rich in macromolecular polymers that exhibit viscoelastic properties. While the influence of viscoelasticity on bacterial swimming is recognized, its impact on chemotaxis-a critical behavior for bacterial survival and colonization-remains elusive. In this study, we employed a microfluidic device to establish attractant gradients and observed the chemotactic behavior of Escherichia coli in both viscoelastic solutions containing carboxymethyl cellulose (CMC) and Newtonian buffers. Our results reveal that E. coli demonstrates markedly enhanced chemotactic efficiency in viscoelastic media. Notably, bacteria achieved faster migration velocities and higher steady-state accumulation in areas with higher attractant concentrations compared to those in Newtonian conditions. Through 3D tracking, we determined that changes in bulk motility parameters alone do not account for the observed enhancements. Further investigations through theoretical analysis and stochastic simulations suggested that the main enhancement mechanisms are mitigation of surface hydrodynamic hindrance resulting from solid surfaces commonly present in bacterial habitats, and the induction of a lifting force in viscoelastic solutions. These findings highlight the significant role of the rheological properties of bacterial habitats in shaping their chemotactic strategies, offering deeper insights into bacterial adaptive mechanisms in both natural and clinical settings.

Drying Behaviour of Al2O3 Inks Containing Carboxymethylcellulose (CMC) for Use in Colloidal Processing

One of the most important steps in the extrusion processing of ceramic inks is the initial drying of the ceramic parts. This study aimed to investigate the drying behaviour of an Al2O3-based ceramic ink optimised to be processed by extrusion processing methods, e.g., direct ink writing. Carboxymethylcellulose (CMC) was singly added to a suspension of deionised water and Al2O3 (50:50 wt.%) to perform as a dispersing and plasticising agent. To assess moisture loss as a function of time, the ceramic inks were extruded into two types of polymeric moulds: one with a completely closed profile producing cylindrical samples (disks) and one with an open profile producing ceramic bars. After the injection of the inks, the moulds were exposed to different controlled temperatures (20 and 40 °C) for up to 180 h; moisture loss and warpage were periodically measured, and exponential mathematical expressions (moisture loss × drying time) were obtained. The Al2O3-bars dried for 24 h in open moulds at 20 and 40 °C presented longitudinal warpages of 4.5% and 9%, respectively, while the Al2O3 disks dried in closed moulds presented warpages of 3.5% and 7% in these same temperatures (20 and 40 °C, respectively). The samples were sintered at 1610 °C for 4 h and characterised by scanning electron microscopy (SEM), relative density (Archimedes principle), and X-ray diffraction (XRD), presenting a relative density of 92.3 ± 0.5%, α-Al2O3 as crystalline phase and grain with equiaxed morphology varying between 1 and 5 μm.

Isolation of cellulose-degrading bacteria from sugarcane bagasse

Introduction: Bagasse in the sugar industry contains a large amount of nutrients and is a very good source of organic fertilizer for plants. However, the main component of sugarcane bagasse is cellulose, which is difficult to decompose. In this study, natural cellulose-degrading bacterial strains were isolated and selected. Methods: Bacteria were isolated from sugarcane bagasse in an environment containing carboxymethyl cellulose (CMC). The cellulase activity of microorganisms is reflected by the size of the halo zone on the medium containing CMC. Results: Among the 8 isolates, three bacterial strains had strong resolving power (halo zones ≥ 20 mm). The strain with the maximum cellulolytic zone diameter was BH01, which exhibited the maximum zone of clearance around the colony with a diameter of 40 mm. The results of molecular identification showed that BH01 is a Bacillus amyloliquefaciens species with 100% similarity to the NICB. The optimal growth conditions for the BH01 strain were a pH of 7 and a temperature of 30°C. Conclusion: Isolated bacterial strains with good cellulose degradation ability can be applied in practice.

Printability of carboxymethyl cellulose/glass-containing inks for robocasting deposition in reversible solid oxide cell applications

Water-based inks containing carboxymethyl cellulose (CMC) and glass particles were investigated in terms of rheological properties and printability for solid oxide cell joining application using robocasting for the first time. The ink formulation containing 36 vol% of glass showed the highest yield stress (9.6 × 102 Pa) and viscosity (4 × 104 Pa.s). CMC-based paste with 37 vol% of glass was selected for printing on Crofer22APU steel substrate due to its relatively low viscosity (2.6 × 104 Pa.s) and high storage modulus (1.4 × 105 Pa), thus ensuring an easier extrusion and shape retention upon printing. The high solid loading of 37 vol% along with optimised debinding and sintering stages enabled achieving high-quality Crofer22APU/glass sealant joining with a reproducible thickness and low porosity.

Novel pseudopolyrotaxane composite based on biopolymers: Synthesis, characterization and application in water treatment

Pseudopolyrotaxane CMC-Na/β-CD (1), polymer composite CMC-Na/TiO2(2) and pseudopolyrotaxane composite CMC-Na/β-CD/TiO2(3) were synthesized for the first time from carboxymethyl cellulose sodium salt (CMC-Na) which synthesized from recycling of sugarcane bagasse, β–cyclodextrin (β-CD) and TiO2 without toxic agent by simple and eco-friendly approach. The synthesized polymers were used in water treatment to remove organic dyes. The structures of novel polymers were characterized by various techniques FTIR, SEM, TGA and XRD measurements. The maximum adsorption for MB could reach 526, 385 and 370 for CMC-Na/β-CD/TiO2, CMC-Na/TiO2 and CMC-Na/β-CD composite respectively. The adsorption results of the composites were fitted with the 2nd order kinetic model (correlation coefficients > 0.99) and the isotherm studies were fitted to the Langmuir model. In the current article successfully prepared promising sustainable composites containing carboxymethyl cellulose (CMC) as new sustainable, cost effective reusable composite and efficient adsorbents for water treatment.

A simple rapid portable immunoassay of trace zearalenone in feed ingredients and agricultural food

Zearalenone (ZEN) has emerged as a global public health concern due to its significant pathogenicity and world-wide distribution in animal feed ingredients and agricultural food. In the study, we report on the design and development of a simple portable immunosensor based on polyamindoamine dendrimer (PD)-multi-walled carbon nanotube (MWCNT) nanocomposite containing carboxymethyl cellulose (CMC) for rapid electrochemical immunoassay of ZEN residual in feed ingredients and agricultural food using U-disk potentiostat with smartphone. A large number of ZEN monoclonal antibody was covalently immobilized onto the electrode surface of nanocomposite owing to abundant carboxyl groups of PD, MWCNT and CMC. Nanocomposite modified screen printed electrode was employed to fabricate a simple rapid portable immunosensor using U-disk potentiostat with smartphone. The electrochemical immunosensor presented a desirable linear relationship ranges from 0.001–100 ng mL−1 with a low detection limit of 0.15 pg mL−1 under the optimal conditions, which was consistent with the results of the immunosensor prepared with glassy carbon electrode on the traditional potentiostat with desktop computer. It is proposed as a simple and robust analytical tool for rapid detection of hazardous substances including ZEN in feed ingredients and agricultural food on the basis of the great performance of the proposed immunosensor.

Isolation of Cellulase Producing Microorganisms from Sugar Bagasse Dump Sites in Lagos, Nigeria

Introduction: Soil is an important reservoir for a diverse group of microorganisms. However, human activities including disposal of solid wastes affects the quality, composition, and microbial communities in the soil. The polymeric units of compounds in a soil determines the type of microbial activities in the soil. Aim: This work investigated the cellulose-degrading potential of the microbial profile of a sugarcane bagasse dumpsite at Ojo Local Government Area of Lagos State. Materials and Methods: The samples collected from this location were labelled A, B, and C respectively based on the soil depth. The samples were processed and inoculated on Nutrient Agar and Potato Dextrose Agar (PDA), to isolate bacteria and fungi. However, the cellulose-degrading ability of the isolates were determined by subculturing the isolates into Mandel’s medium containing Carboxymethyl cellulose (CMC). Results: The physiochemical analysis of the soil samples revealed the variations in parameters such as pH, moisture, nitrogen, organic carbon etc, at different soil depth. Four cellulose-degrading bacteria and fungi were isolated. The isolated bacterial species are Bacillus species, Serratia species, Pseudomonas species and Lactobacillus species. Bacillus species had the highest cellulose-degrading potential amongst the four isolates with Serratia species having the lowest. The isolated fungal species are Aspergillus niger, Penicillium species, Mucor species, Neurospora species, Microsporium species and Aspergillus flavus. Conclusion: In this work, we demonstrated successfully that soil from sugarcane bagasse dumpsite is a rich source of cellulose and possesses a high rate of activities of cellulolytic bacteria and fungi. The major deduction from this work is that Bacillus species and Mucor species are prominent cellulolytic microorganisms and have immense potential for industrial applications.

Hydroxyapatite-carboxymethyl cellulose-graphene composite coating development on AZ31 magnesium alloy: Corrosion behavior and mechanical properties

In this study, hydroxyapatite (HA) coatings containing carboxymethyl cellulose (CMC) and graphene (Gr) were developed on AZ31 magnesium alloy through two-step electrophoretic deposition method. The morphology and chemical bonding of coatings were characterized and also the phase identification was done using scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction, respectively. Moreover, the corrosion behavior of the applied coatings was compared with the bare AZ31 Mg alloy substrate in the simulated body fluid by the means of potentiodynamic polarization test and electrochemical impedance spectroscopy. Obtained results revealed that the novel HA-CMC-Gr coating possesses the highest corrosion resistance compared to the HA, HA-CMC, and HA-Gr coatings due to its uniform and compact structure. To investigate the mechanical properties and to elucidate the effect of CMC on the adhesion of coating-alloy interface, pull-off test was employed, where results demonstrated that the addition of CMC increases the adhesion force from 1.06 MPa to 1.62 MPa. Besides, the modulus of elasticity and the hardness of HA and HA-Gr composite coatings were compared by applying nanoindentation test. Interestingly, it is detected that the presence of Gr has considerably increased the elastic modulus of the coating by approximately 30% in comparison to the pure HA coating.

In Vitro and Ex Vivo Evaluation of Nepafenac-Based Cyclodextrin Microparticles for Treatment of Eye Inflammation.

The aim of this study was to design and evaluate novel cyclodextrin (CD)-based aggregate formulations to efficiently deliver nepafenac topically to the eye structure, to treat inflammation and increase nepafenac levels in the posterior segment, thus attenuating the response of inflammatory mediators. The physicochemical properties of nine aggregate formulations containing nepafenac/γ-CD/hydroxypropyl-β (HPβ)-CD complexes as well as their rheological properties, mucoadhesion, ocular irritancy, corneal and scleral permeability, and anti-inflammatory activity were investigated in detail. The results were compared with a commercially available nepafenac suspension, Nevanac® 3 mg/mL. All formulations showed microparticles, neutral pH, and negative zeta potential (–6 to –27 mV). They were non-irritating and nontoxic and showed high permeation through bovine sclera. Formulations containing carboxymethyl cellulose (CMC) showed greater anti-inflammatory activity, even higher than the commercial formulation, Nevanac® 0.3%. The optimized formulations represent an opportunity for topical instillation of drugs to the posterior segment of the eye.

Isolation, screening and identification of potential cellulolytic and xylanolytic mold from oil palm waste

The objective of this research was to isolate, screen, and identify the cellulase and xylanase-producing mold from oil palm waste. There are thirty-two isolates from oil palm waste which are able to degrade and grow on media containing cellulose or xylan as a sole carbon source. Screening to determine cellulolytic and xylanolytic activity was performed by paper disc diffusion method using Congo Red as an indicator. All the thirty-two mold isolates showed cellulolytic and xylanolytic activity with relative enzyme activity (ratio of hydrolysed zone diameter and diameter of colony growth) ranging from 1.04 to 1.62. Based on the macro- and micromorphology characteristics, these isolates were identified as genus Trichoderma, Aspergillus, Rhizopus, Tallaromyces and Penicillium, with the number isolates in each genus was 22, 4, 3, 2, and 1 respectively. The highest cellulolytic and xylanolytic activity was achieved from the isolate namely OPT1(4) which was identified as Talaromyces pinophilus using morphological and molecular analysis. Under the optimum condition (30°C, 72 hours) in liquid medium containing carboxymethyl cellulose (CMC) and birch wood xylan as the main carbon source, Talaromyces pinophilus OPT1(4) presented glucanase and xylanase activity of 15.17 U/mL and 13.35 U/mL, respectively.

An artificial tear containing flaxseed oil for treating dry eye disease: A randomized controlled trial

PurposeTo evaluate the efficacy and safety of a nano-emulsion artificial tear (OM3) containing carboxymethylcellulose (CMC) and glycerin, flaxseed oil and castor oil, and three osmoprotectants (levocarnitine, erythritol, and trehalose) compared with an artificial tear (Refresh Optive Advanced [ROA]) containing the same ingredients with the exception of trehalose and flaxseed oil. MethodsIn this multicenter, double-masked, randomized, two-arm, parallel-group, 6-visit study (screening, baseline, and days 7, 30, 60, and 90), subjects with dry eye disease underwent an open-label, 7-day run-in with CMC 0.5% (Refresh Plus), before 1:1 randomization to OM3 or ROA for 90 days (both instilled ≥2 daily). Ocular Surface Disease Index (OSDI; primary endpoint change from baseline at day 90), tear film breakup time (TBUT), and ocular staining (combined/corneal/conjunctival) were assessed; change from baseline in these parameters was calculated at each timepoint. Treatment-related adverse events (AEs) were assessed at each visit. ResultsOverall, 242 subjects were randomized (OM3, n = 120; ROA, n = 122). At day 90, significant improvements in OSDI, ocular staining and TBUT were evident in both treatment groups. Significant (P < 0.05) between-group differences in favor of OM3 were observed for combined ocular staining (all timepoints), corneal staining (day 90), and conjunctival staining (day 30). Treatment-related AEs were higher in the ROA (9.8%) versus OM3 (6.7%) group; blurred vision was among the most commonly reported AE (OM3 0% vs ROA 4.1%). ConclusionThese findings support the application of OM3, a novel preservative-free, nano-emulsion tear formulation with trehalose and flaxseed oil, for the treatment of dry eye disease.

Effect of CuO nanofiller on the spectroscopic properties, dielectric permittivity and dielectric modulus of CMC/PVP nanocomposites

System of nanocomposite films containing carboxymethyl cellulose (CMC) and polyvinyl pyrrolidone (PVP) embedded copper oxide (CuO) nanoparticles were synthesized using the casting method solution and it was described by appropriate techniques. The semicrystalline nature of the structure for the nanocomposites was confirmed by X-ray spectra. The main characteristic bands of IR groups of CMC and PVP were clearly founded in the IR spectra. The intensity of some bands was reduced confirms a well reacts between CuO the main IR groups inside CMC/PVP polymer were occurring. The estimations of M′ were studied according to the conduction phenomenon related to short-range mobility of charge carriers. Minimum values of M″ at lower frequency were attributed to the indication of transport for ions with one relaxation peaks were founded which increase with an increase of CuO attributed to the relaxation process. The presence of a semicircle shape in the relation between M′ and M″ was observed demonstrating the presence of broad relaxation processes. The behaviour of ɛ′ was reduced as an increase of the frequency ascribed to polarization effects and it reaches to constant values at a higher frequency according to the contribution for charge accumulation. The behaviour of Z′ and Z″ was reduced as the increase of frequency as well as CuO. The magnitude of tanδ was also reduced because the hopping of charge carriers does not cause any changes of the applied field.

ISOLASI DAN SKRINING BAKTERI SELULOLITIK DARI BEBERAPA TANAH HUTAN DI BALI

Soil is one habitats of the cellulolytic bacteria. Soil containing manure and decayed wood is habitation for cellulolytic bacteria. This research is aimed to isolate and screen cellulolytic bacteria from the soil in Bali that has the potentials to degrade cellulose. The soil samples ware taken from forest in Gunaksa-Klungkung, Telaga-Karangasem, Sukahat-Karangasem, Gilimanuk-Jembrana, and Mangrove forests in Suwung-Denpasar. Each single cellulolytic bacteria colonies which grown on solid media containing carboxymethyl cellulose (CMC) were isolated. Screening of cellulolytic bacteria using congo red 0,1 %, staining bacterial isolates which were created clear zones in around the colony were selected. The isolation result are 67 isolated cellulolytic bacteria covered in five soils sample. Twenty-one bacterial isolates were producing cellulase enzymes with an indication of a clear zone in around the colony. Nine superior isolates have high cellulolytic index (CI), namely: G2-8 (5.41), G1-4 (4.86), G2-10 (4.5), G2-2 (3.64), M1-5 (3.10), G2-5 (3.03), M2-12 (2.72), G1-1 (2.38), and M1-1 (2.21). The obtained highest percentage of filter paper (Whatman No.1) degradation was 8.32% and the lowest was 2.48%.&#x0D; Keywords: forest soil, isolation, cellulolytic bacteria, congo red

Isolation and Identification of Cellulolytic Bacteria from Wood Industry Soil in Kancheepuram, Chennai

Cellulose degradation is carried out by a large number of microorganisms with only a limited population of these organisms producing a substantial amount of cellulase capable of complete hydrolysis of cellulose; cellulolytic bacteria are one of the main cellulase-producing microorganisms. A total of 22 bacterial strains from 3 different wood industries in Chennai was isolated on agar plates containing carboxymethyl cellulose (CMC) as the substrate, two strains showed hydrolyzing zones after congo red staining and microscopic examination of the isolates revealed Gram-positive cocci exhibiting pink coloured cells; using 16S rRNA sequence, phylogenetic analysis was performed with BLAST program to determine the evolutionary relationship and molecular identification indicated that 2 strains (ABTRI-1 and ABTRI-2) exhibit 99% similarities to Oceanobacillus profundus on the basis of 16S rRNA gene sequence analysis. Identification of bacterial species capable of cellulose degradation is becoming an important aspect in the mass production of cellulase enzymes that have applications in textile industries, food industries as well as biomedical fields.

Carboxymethyl cellulose/polyacrylamide composite hydrogel for cascaded treatment/reuse of heavy metal ions in wastewater

A new bio-based bilateral hydrogel containing carboxymethyl cellulose (CMC) and polyacrylamide (PAM) was prepared for the wastewater remediation. The obtained CMC/PAM composite hydrogel reveals a strong single-ion affinity for copper (CuII), lead (PbII) and cadmium (CdII) ions, as well as multi-ion absorbability with its equilibrium data following the Langmuir adsorption model and its adsorption process abiding by a pseudo-second-order kinetics. To demonstrate its recycled use for the metal ions, the adsorbed CuII ions in the hydrogel were reduced in situ to form homogeneously dispersed Cu nanoparticles (NPs), leading to the Cu NPs-loaded CMC/PAM hydrogel. Reduction of 4-nitrophenol to 4-aminophenol with high efficiency was successfully achieved by this Cu NPs-loaded CMC/PAM hydrogel, verifying the hydrogel's dual functionalities towards the wastewater treatment and catalytic application.

Unraveling the cellulolytic and hemicellulolytic potential of two novel Streptomyces strains

The Streptomyces spp. are notorious plant biomass decomposers in soil environments, but only few strains were biochemically and genetically characterized. Here, we employed functional screening along with genomic sequencing for identification of novel lignocellulolytic Streptomyces strains. Streptomyces strains isolated from soil were functional screened based on their cellulolytic and hemicellulolytic capacities by enzymatic plate assays containing carboxymethylcellulose (CMC) and beechwood xylan as sole carbon source. Subsequently, genomes of Streptomyces strains were sequenced, annotated, and interpreted to correlate their genetic contents with biochemical properties. Among the 80 bacterial isolates that were screened for enzymatic activity, two Streptomyces strains (named as F1 and F7) exhiting higher endoglucanase and endoxylanase activities were selected for biochemical and genomic characterization. After cultivation on steam-pretreated sugarcane bagasse-based medium, the supernatant of the strains F1 and F7 exhibited enzymatic activity against different substrates, such as arabinan, rye arabinoxylan, β-glucan, starch, CMC, xylan, and chitin. Furthermore, strain F7 was able to degrade pectin, mannan, and lichenan. The genomic analysis of both strains revealed a diversity of carbohydrate-active enzymes. The F1 and F7 genomes encode 33 and 44 different types of glycosyl hydrolases families, respectively. Moreover, the genomic analysis also identified genes related to degradation of lignin-derived aromatic compounds. Collectively, the study revealed two novel Streptomyces strains and further insights on the degradation capability of lignocellulolytic bacteria, from which a number of technologies can arise, such as saccharification processes.

Optimization and characterization of extracellular cellulase produced by Bacillus pumilus MGB05 isolated from midgut of muga silkworm (Antheraea assamensis Helfer)

Abstract Mature larvae of Antheraea assamensis were collected from different locations of Assam to isolate the cellulolytic gut microflora. Altogether sixty cellulase degrading bacteria were isolated on agar plates containing microcrystalline cellulose as the sole carbon source. Among them, ten isolates showed hydrolyzing zone on agar plates containing carboxy methyl cellulose (CMC) after staining with Congo-red. Isolate MGB05 exhibited the highest CMCase activity (0.262 U/mL) at 72 h of incubation under submerged condition. FPase and β-glucosidase activity were 0.012 U/mL and 3.71 U/mL respectively. It showed maximum FPase (0.022 U/mL) activity on the 3rd day of incubation in the media containing wheat bran as a carbon source. β-glucosidase production was also found to be highest with wheat bran (20.03 U/mL) at 48 h of incubation. The optimum pH and temperature of FPase activity of MGB05 were found at 6.0 and 50 °C respectively while for β-glucosidase activity, it was maximum at pH 6.0 under 50 °C. In addition, metal ion Mg++ and Ca++ enhanced FPase activity up to 110.92% (0.026 U/mL) and 105.31% (0.025 U/mL) respectively. In-vitro antimicrobial bioassay of the most potent cellulolytic bacteria (MGB05) also showed high antimicrobial activity against Escherichia coli (2.9 cm) and Pseudomonas aeruginosa (3.0 cm). The isolate MGB05 has been identified based on 16S rDNA homology as Bacillus pumilus MGB05 with accession KP298708.2. Results encompass the prospective beneficial role of gut-microflora on digestion and disease resistance, which might be a potential probiotic component to enhance silk productivity.