Plant Protein Consumption Research Articles

Exploring the biodiversity of plant proteins for sustainable foods: Composition and emulsifying properties of the proteins recovered by aqueous extraction from camelina (Camelina sativa L.) seeds

The food transition towards an increased consumption of plant proteins aimed at limiting environmental impacts requires a diversification of plant protein sources. In this study, we explored the potentialities of the sustainable oilseed crop camelina to provide dietary proteins and to prepare oil-in-water emulsions. An innovative green refinery process, including the removal by ultrasound of the mucilage attached at the surface of the seeds and extraction by grinding in water at pH 8, was used to recover aqueous extracts containing camelina seed proteins. These proteins, mainly composed of the 11S globulin cruciferins and the 2S albumin napins, contained the nine essential amino acids of nutritional interest. Camelina seed proteins exhibited low solubility in the range of pH H 5.5-2, attributed to the cruciferins. Oil-in-water emulsions stabilized by camelina seed proteins revealed the preferential adsorption of napin at the oil/water interface and exhibited physical instability below pH 7. Homogenization of the whole aqueous extracts containing oil bodies (OBs) induced the adsorption of cruciferins and napins together with oleosins at the surface of homogenized OBs, and their sedimentation below pH 6.5. Camelina seed proteins adsorbed at the oil/water interface governed the surface properties of the oil droplets and homogenized OBs and the physical stability of the emulsions as a function of pH. This work brings new insights into the potentialities of camelina seed proteins recovered by aqueous extraction that may serve as natural plant-based functional ingredients in the food industry and that will contribute to the development of innovative and sustainable plant-based food emulsions.

BMI Mediates the Association between Macronutrient Subtypes and Phenotypic Age Acceleration.

Background: There is growing evidence that diet and aging are associated; however, few studies have examined the relationship between macronutrient subtypes and phenotypic age acceleration, and the extent to which BMI (body mass index) mediates this association is unclear. Methods: This study included 6911 individuals who were 20 years or older and had participated in the National Health and Nutrition Examination Survey. Daily macronutrient intakes were calculated and classified by the quartile of their subtypes. PhenoAgeAccel was calculated as an aging index using nine chemistry biomarkers. Multivariable linear regression and isocaloric substitution effects were used to evaluate the association of macronutrients with PhenoAgeAccel. Mediation analyses were used to examine the mediation role of BMI in the association. Results: After adjusting for the potential covariates, the consumption of high-quality carbohydrates (β = -1.01, 95% CI: -1.91, -0.12), total protein (β = -2.00, 95% CI: -3.16, -0.84), and plant protein (β = -1.65, 95% CI: -2.52, -0.78) was negatively correlated with PhenoAgeAccel; the consumption of SFAs (β = 1.77, 95% CI: 0.72, 2.81) was positively correlated with PhenoAgeAccel. For every serving of low-quality carbohydrates/animal protein and other calories replaced by one serving of high-quality carbohydrates/plant protein, PhenoAgeAccel would be reduced by about 25 percent. The ratio between BMI-mediated high-quality carbohydrates and PhenoAgeAccel accounted for 19.76% of the total effect, while the ratio between BMI-mediated total fat and PhenoAgeAccel accounted for 30.78% of the total effect. Conclusions: Different macronutrient consumption subtypes are related to PhenoAgeAccel, which is partially mediated by BMI, depending on the quality of macronutrients. Replacing low-quality macronutrients with high-quality macronutrients might slow aging.

Animal and plant protein intake association with mental health, tryptophan metabolites pathways, and gut microbiota in healthy women: a cross-sectional study

Mental health is affected by tryptophane (TRP) metabolism regulation. Diet-influenced gut microbiome regulates TRP metabolism. Thus, the present study aimed to explore the relationship between type of dietary protein intake, gut microbiota, TRP metabolites homeostasis, and mental well-being in healthy women. 91 healthy females aged 18–50 were recruited based on the study protocol. Validate and reliable questionnaires assessed dietary intake and mental health. Biochemical tests and gut microbiota composition were analyzed following the manufacturer’s instructions for each enzyme-linked immune sorbent assay (ELISA) kit and Real-time quantitative polymerase chain reaction (qPCR) methods respectively. Regression methods were used to estimate the considered associations. The results show that in the fully adjusted model, plant protein consumption was partially inversely associated with depression risk (OR = 0.27; 95% CI: 0.06, 1.09; P = 0.06). Higher dietary animal protein intake was marginally associated with psychological distress (OR = 2.59; 95% CI: 0.91, 7.34; P = 0.07). KYN to serotonin ratio was inversely associated with animal protein consumption (ß = 1.10; 95% CI: -0.13, 2.33; P = 0.07). Firmicutes/Bacteriodetes ratio (β = -1.27 × 103, SE = 5.99 × 102, P = 0.03) was lower in the top tertile of plant protein. A partially negative correlation was found between dietary animal protein and Prevotella abundance (β = -9.20 × 1018, SE = 5.04 × 1018, P = 0.06). Overall, significant inverse associations were found between a diet high in plant protein with mental disorders, KYN levels, and Firmicutes to Bacteroidetes ratio while adhering to higher animal protein could predispose women to psychological stress.

Unraveling the Shift: Exploring Factors Influencing Protein Preference and Consumption Patterns in Ga South Municipality of Ghana

This study endeavors to elucidate the factors influencing protein preference and consumption patterns in the Ga South Municipality of Ghana. Understanding these determinants is pivotal for promoting environmentally sustainable and healthy dietary habits. Utilizing a random sampling approach, the study collected 150 meticulously structured questionnaires from a representative sample of municipality residents. It delved into the socio-cultural and economic factors shaping protein preferences and consumption behaviors, alongside investigating the impact of awareness, knowledge, and availability of protein sources on dietary choices. The findings unveiled that respondents partake of protein daily, albeit with variations in patterns based on sources: plant protein consumption occurred weekly, while animal protein was consumed daily. Moreover, respondents exhibited awareness of the nutritional benefits associated with protein consumption. While awareness for protein nutrition is high, the price remains a deterrent to accessing protein in Ga South Municipality. Calling on policymakers to support local food production and supply, invest in food literacy, and develop good transportation to reduce logistics bottlenecks that often create a massive difference between farm gate and market prices.

Association Between Healthy Eating Index-2020 and Oral Microbiome Among Postmenopausal Women

BackgroundDietary intake has been suggested to be associated with the oral microbiome, but no study has examined the association between overall diet quality and the oral microbiome. ObjectivesThis study aimed to investigate the cross-sectional association between the Healthy Eating Index-2020 (HEI-2020) and the diversity and composition of the oral microbiome among participants in the Buffalo Osteoporosis and Periodontal Disease (OsteoPerio) Study. MethodsIn 1175 postmenopausal women (mean age: 67 ± 7.0 y), we estimated the HEI-2020 scores for each woman from a food frequency questionnaire administered from 1997 to 2000. Bacterial DNA was extracted from subgingival plaque samples and analyzed using 16S ribosomal RNA sequencing. The alpha-diversity (within-sample diversity) and β-diversity (between-sample diversity) across HEI-2020 quartiles were examined using analysis of covariance and permutational multivariate analysis of variance, respectively. The associations between the HEI-2020 score and the relative abundance of microbial taxa were examined by linear regression models. The analyses were further conducted for individual components of the HEI-2020. ResultsNo statistically significant associations were observed between the HEI-2020 scores and alpha- or beta-diversity. However, greater consumption of seafood, plant proteins, and total protein and lower consumption of added sugars were positively associated with alpha-diversity. After we applied a false detection rate (FDR) correction, higher HEI-2020 scores were significantly associated with decreased abundance of Lautropia, Streptococcus gordonii, Cardiobacterium valvarum, and Cardiobacterium hominis, and increased abundance of Selenomonas sp. oral taxon 133 and Selenomonas dianae (FDR-adjusted P values < 0.10). Additionally, 28 other taxa were identified as being associated with HEI-2020 components. ConclusionsAlthough the HEI-2020 was associated with the composition, but not the diversity, of the oral microbiome, individual HEI-2020 components were associated with both its diversity and composition. Specific dietary components may have more impact on the diversity and composition of oral microbiome than overall diet quality assessed by the HEI-2020.

Enhanced dietary reconstruction of Korean prehistoric populations by combining δ13C and δ15N amino acids of bone collagen.

Compound specific stable isotope analysis of amino acids (CSIA-AA) is a powerful tool for determining dietary behaviors in complex environments and improving dietary reconstructions. Here, we conducted CSIA-AA on human (n = 32) and animal (n = 13) remains from two prehistoric archaeological sites (Mumun, Imdang) to assess in more detail the dietary sources consumed by prehistoric Korean populations. Results of estimated trophic position (TP) using Δ15NGlx-Phe show that the Imdang individuals consumed aquatic resources, as well as terrestrial resources. Principal component analysis (PCA) using δ13C and δ15N essential amino acid (EAA) values show that the Imdang humans closely cluster with game birds and terrestrial herbivores, whilst the Mumun humans closely cluster with C4 plants. Quantitative estimation by a Bayesian mixing model (MixSIAR) indicates that the Imdang humans derived a large proportion of their proteins from terrestrial animals and marine fish, whereas the main protein sources for the Mumun humans were C4 plants and terrestrial animals. Additionally, the comparison between the EAA and bulk isotope models shows that there is a tendency to overestimate the consumption of plant proteins when using bulk isotopic data. Our CSIA-AA approach reveals that in prehistoric Korea there were clear differences in human diets through time. This study adds to a growing body of literature that demonstrates the potential of CSIA-AA to provide more accurate estimations of protein consumption in mixed diets than previous bulk isotopic studies.

Seed storage allergens tackled via next-generation research assistant

The expanding consumption of plant proteins in the diet to overcome the environmental issues associated with animal proteins is increasing the incidence of food-induced allergic reactions. One of the 21st-century research drivers in agriculture sciences is the development and validation of concrete approaches for modulating the expression of allergenic proteins in crops before harvesting. The increasing incidence of plant food allergies is primarily induced by seed storage proteins that clinicians are experiencing recently because of the more predominant use of plant-derived proteins in the food industry. Increased availability of high-throughput technologies has generated an ever-growing number of omics data, allowing us to have better structural knowledge of SSPs and molecular properties that can inform the allergenicity assessment. The recent systems for targeted genome engineering, without double-strand DNA breaks, allow the introduction of precise modifications directly into commercial plant species. Artificial intelligence is significantly transforming scientific research across every stage, assisting scientists, processing large-scale data, making predictions, automating tasks. During this epochal change, marked by the encounter between artificial intelligence and synthetic biology, a next-generation research assistant (NGA) is coming alive. Here, we propose a new conceptual vision to facilitate and speed up the editing of cross-reactivity sites to obtain hypoallergenic cultivars and avoid pleiotropic effects. Finally, we discuss the potential applications of this new way to conceive the research. NGA may be undoubtedly capable of managing the evolution of SPP allergies through the prediction of novel epitopes, as well as the prediction of immunological response mechanisms.

Relationships between diet and gut microbiome in an Italian and Dutch cohort: does the dietary protein to fiber ratio play a role?

To investigate the relationships between the habitual diet, the protein to fiber ratio (P/F), and the gut microbiome in one Italian and one Dutch cohort of healthy subjects consuming an omnivore diet. The Italian cohort included 19 males (M_IT, BMI 25.2 ± 0.72kg/m2, age 25.4 ± 0.96years) and 20 females (F_IT, BMI 23.9 ± 0.81kg/m2, age 23.8 ± 0.54years); the Dutch cohort included 30 females (F_NL, BMI: 23.9 ± 0.81kg/m2, age: 23.8 ± 0.54years). Individual diets were recorded through Food Frequency Questionnaires and analyzed to assess the nutrient composition. Gut microbiome was assessed in fecal samples. M_IT consumed higher levels of proteins than F_NL and F_IT, whereas dietary fiber intake did not differ among groups. Data showed that consumption of plant protein to animal protein (PP/AP) and PP to total proteins ratio can determine a differentiation of F_NL more than the absolute amount of dietary fiber. Conversely, the protein to fiber (P/F) and AP to total proteins better characterized M_IT. M_IT harbored the highest abundance of proteolytic microorganisms and the lowest microbial gene richness. Conversely, F_NL had more fiber-degrading microorganisms like Bacteroides thetaiotaomicron, Bacteroides xylanisolvens, Roseburia sp., Coprococcus eutactus and Parabacteroides along with the highest number of genes encoding carbohydrate-active enzymes and gene richness. It was predicted that by each unit decrease in the P/F a 3% increase in gene richness occurred. Study findings suggested that dietary P/F, rather than the absolute amount of dietary fiber, could contribute to the shaping of the microbiome towards a more proteolytic or fiber-degrading gut ecosystem. gov Identifier NCT04205045-01-10-2018, retrospectively registered. Dutch Trial Register NTR7531-05-10-2018.

Animal protein intake is directly associated with serum level of pentraxin 3 in hemodialysis patients

Inflammation plays an important role in Cardiovascular disease (CVD) pathogenesis as the main cause of mortality in hemodialysis (HD) patients. Despite the relevance of nutrition and dietary intakes for inflammation status, the role of dietary protein sources remains unclear. The aim of this study was to evaluate the association between the different types of dietary protein and pentraxin 3 (PTX3) levels in HD patients. In this multi-center cross-sectional study, 227 adult patients undergoing HD for a minimum 90 days were recruited. A validated 168-item food frequency questionnaire was used to assess dietary intakes. Also, 5 ml blood samples were collected from each patient to measure the concentration of serum PTX3. Overall, 227 patients, including 63 women and 164 men, with a mean age of 58 years, participated in this study. There was a greater intake of animal protein per kilogram dry weight among patients with higher levels of PTX3 (0.46 vs. 0.54 g/kg; P = 0.035). In contrast, consumption of total protein and plant protein per kilogram dry weight was not different across PTX3 levels. Moreover, the chance of increased PTX3 concentration was directly associated with a one-unit increase in animal protein intake per kilogram dry weight, after adjusting for confounders. We did not observe any association between one-unit increases in plant protein intake per kilogram dry weight and chance of increased PTX3. In conclusion, animal protein intake was directly associated with circulating PTX3.

Zero-Inflated Poisson Regression Modeling of Plant Protein Consumption

Summary This research fitted a discrete distribution for modeling count data. Specifically, Zero-Inflated Poisson (ZIP) regression was used to model plant protein consumption by 400 randomly sampled individuals in Wukari. The data was collected by questionnaire. The ZIP regression model was used based on its ability to model data with excess zeros present in the collected data. Variables considered and used for the analysis are Age, Body Mass Index, Blood Pressure, Occupation, Gender, Weight, Height, Body Reaction, and Consumption Class. The parameters of the ZIP model were estimated using the maximum likelihood estimation technique. The model was tested for Goodness of Fit (GoF) using deviance, scaled deviance, Pearson–χ 2 and scaled Pearson–χ 2 statistics. The results obtained showed that Age, Gender, and Reaction were significant at 5%, and the GoF tests revealed that the Zero-Inflated Poisson regression produces a good fit and is a good model for overcoming the overdispersion effect.

Plant protein consumption is associated with body mass index among women of reproductive age in Indonesia

IntroductionOne of the known determinants of obesity in Southeast Asia countries, including Indonesia, is the nutritional transition, which is indicated by fast changes in food production, dietary habits, and physical activity. With rising incomes, plant protein from grains, tubers, and legumes is gradually being replaced by animal protein from poultry, eggs, dairy, and red meat. This change is identified as a protein transition. Different choices of protein sources in the diet have varying health effects. However, there is limited information on the Asian population on the role of protein consumption on the increasing obesity prevalence. Therefore, this study aimed to investigate the association of protein sources consumption with body mass index (BMI) among women of reproductive age in Indonesia.MethodsThis study used secondary data from the 2018 Indonesia Food Barometer (IFB) conducted using a quantitative cross-sectional survey. A total of 467 Indonesian reproductive-aged women (20–49 years) were included in this study. Dietary intake, including protein consumption, was obtained using 24-h dietary recall. Multiple linear regression was applied to find the association of protein consumption with BMI with a p-value &amp;lt;0.05 considered as a significant outcome variable.ResultsThe Mean BMI was 25.02 kg/m2, median of animal and plant protein was 28.01 g/day and 25.37 g/day, respectively. Consumption of plant protein was significantly associated with BMI after adjusting for marital status and age (p-value = 0.043; R2 = 0.080). The quality of plant protein should be considered to prevent obesity problems among women of reproductive age.

Relationship Between Protein Types and hs-CRP on eGFR

Few studies have measured the relationship between protein types and inflammation on kidney function among US adults. The objective was to identify the relationship between protein types (animal, dairy, plant, seafood) and high-sensitivity C-reactive protein on estimated glomerular filtration rate (eGFR). A cross-sectional secondary analysis using NHANES 2017-2018 data was performed on 4252 adults with an eGFR of &gt;15 ml/min/1.73m2. At least one 24-hour recall was used to categorize the protein type. Descriptives, frequencies, Pearson correlation, and multiple linear regressions were conducted using SPSS v28 with a significance of p&lt;0.05. Participants were mostly female (50.1%), had a mean age of 50.1 years, and self-identified as non-Hispanic White (36.5%). A majority consumed animal and dairy products compared to seafood and plant products. A negative association was observed between consumption of plant proteins and inflammation on eGFR (p&lt;0.05). In the adjusted regression model, non-Hispanic Black race had a positive influence on eGFR, while female and age had negative influences on eGFR (p &lt; 0.001). Reduced consumption of plant proteins with elevated inflammation can decline kidney function. Future studies should focus on frequency and amount of proteins consumed and inflammation to reduce the progression of chronic diseases such as kidney disease.

Environmentally sustainable food: an analysis of consumer practices and their diffusion in France

PurposeThis aim of this study is to identify the ways of helping public authorities bring about change to environmentally sustainable household food practices.Design/methodology/approachThe authors identified the practices involved in this concept from the consumer perspective and measured their diffusion among French households. The analyses were conducted following two successive data collection campaigns comprising 571 and 501 respondents in France. The methodology involved two complementary scaling techniques: factor analysis and item response theory.FindingsThe results show that consumers understand sustainable food through five food practices: buying and cooking products with sustainable attributes, anti-waste storage, self-production, plant protein consumption and anti-waste cooking.Originality/valueThe findings suggest that while at the individual level people appear to have incorporated anti-waste practices into their daily lives, at the household level, there is still work to be done for improving diets and stimulating the production of home-grown food. It is also worth noting that the emerging vision typically involves sustainable foods that are organic, locally grown, seasonal, based on fair trade and packaging-free.

Plant Protein but Not Animal Protein Consumption Is Associated with Frailty through Plasma Metabolites.

There is evidence that the association of protein intake and frailty may depend on the source of dietary protein. The mechanism underlying this association is not clear. In this study, we explore circulating metabolites as mediators of the relationship between dietary protein and of frailty in participants of the Baltimore Longitudinal Study of Aging (BLSA). Cross-sectional analyses in 735 BLSA participants of associations between plant and animal protein intake and frailty. Usual protein intake from plant and animal sources were estimated with a Food Frequency Questionnaire (FFQ) and frailty was assessed with a 44-item Frailty Index (FI). Compared with the lowest quartile, higher quartiles of plant, but not animal, protein were associated with lower FI. Twenty-five plasma metabolites were associated with plant protein intake; of these, fifteen, including phosphatidylcholines, cholesterol esters, sphingomyelins, and indole metabolites, mediated the association between plant protein intake and FI. The protective association between plant protein consumption and FI is mediated by lower abundance of lipid metabolites and higher abundance of tryptophan-related metabolites.

Barriers associated with pulse and plant-based meat alternative consumption across sociodemographic groups: a Capability, Opportunity, Motivation, Behaviour model approach.

To enhance environmental sustainability and food security, there should be a change in dietary protein consumption. It is suggested that meat consumption should be reduced and that the currently low consumption of pulses and other plant-based proteins should increase. We aimed to examine (1) how sociodemographic factors and perceived barriers are associated with self-reported current and perceived future pulse and other plant-based meat alternative (PBMA) consumption and (2) how sociodemographic factors relate to perceived barriers. Participants were 18-75 year-old Finnish adults (n = 1,000). Multivariable logistic regression was used as the main analysis technique. The results were interpreted by employing the Capability, Opportunity, Motivation, Behaviour (COM-B) model. Pulses were consumed more often than PBMAs and lower education level and financial strain were associated with more infrequent pulse and PBMA use. The most common perceived barriers for pulse consumption were unfamiliarity, expensive price, and unpleasant taste, which can be interpreted to represent the capability, opportunity and motivation components of the COM-B model, respectively. Women, the young, and financially strained perceived more barriers limiting their pulse consumption than others. To increase plant-based food consumption, it is important that tasty, easy to use and affordable plant-based foods are available for all. Additionally, we suggest that food services should be encouraged to increase the use of pulses in their dishes and that capabilities, opportunities and motivations are taken into account in intervention measures advancing plant protein consumption.

Transforming sustainable plant proteins into high performance lubricating microgels

With the resource-intensive meat industry accounting for over 50% of food-linked emissions, plant protein consumption is an inevitable need of the hour. Despite its significance, the key barrier to adoption of plant proteins is their astringent off-sensation, typically associated with high friction and consequently poor lubrication performance. Herein, we demonstrate that by transforming plant proteins into physically cross-linked microgels, it is possible to improve their lubricity remarkably, dependent on their volume fractions, as evidenced by combining tribology using biomimetic tongue-like surface with atomic force microscopy, dynamic light scattering, rheology and adsorption measurements. Experimental findings which are fully supported by numerical modelling reveal that these non-lipidic microgels not only decrease boundary friction by an order of magnitude as compared to native protein but also replicate the lubrication performance of a 20:80 oil/water emulsion. These plant protein microgels offer a much-needed platform to design the next-generation of healthy, palatable and sustainable foods.

Plant Proteins: Methods of Quality Assessment and the Human Health Benefits of Pulses.

As countries increase their standard of living and individual income levels rise, there is a concomitant increase in the demand for animal-based protein. However, there are alternative sources. One of the alternatives available is that of increased direct human consumption of plant proteins. The quality of a dietary protein is an important consideration when discussing the merits of one protein source over another. The three most commonly used methods to express protein quality are the protein efficiency ratio (PER), a weight gain measurement; protein digestibility-corrected amino acid score (PDCAAS); and the digestible indispensable amino acid score (DIAAS). The possibility that alterations in the quality and quantity of protein in the diet could generate specific health outcomes is one being actively researched. Plant-based proteins may have additional beneficial properties for human health when compared to animal protein sources, including reductions in risk factors for cardiovascular disease and contributions to increased satiety. In this paper, the methods for the determination of protein quality and the potential beneficial qualities of plant proteins to human health will be described.

Speeding up the transition to a more sustainable food system: New insights into the links in a system of practices

More sustainable food choices are part of a complex system that combines practices, which themselves are interconnected in a network of elements made up of competences, meanings and material devices. This article proposes an innovative method for studying this system through a non-deductive quantitative approach. The research adds to the corpus of the ‘theory of practices’ by defining the concepts of ‘central’ practices (i.e. connected to many elements of the system, such as the practice of buying and cooking sustainable products in the sustainable food system) and ‘peripheral’ practices (i.e. connected in a lesser extent to other elements of the system, such as the practices of anti-waste, growing your own food and the consumption of plant proteins). It also highlights the ‘connector’ role between the practices of certain elements. Finally, based on the current structure of the system of sustainable food practices, the article makes several propositions for speeding up change.

The dietary requirement for total sulfur amino acids in adults aged ≥60 years appears to be higher in males than in females

BackgroundThe total sulfur amino acid (TSAA) recommendation in older adults is based on data from young adults. Physiological evidence suggests that older adults have a higher requirement than young adults. ObjectivesThe objective of this study was to determine the TSAA requirement in healthy men and women aged ≥60 y. MethodsThe TSAA requirement was determined using the indicator amino acid oxidation method with L-[1-13C]phenylalanine as the indicator. At recruitment, 15 older adults (n = 7 men and n = 8 women; BMI < 30 kg/m2) were assigned to receive 7 methionine intakes (5, 10, 15, 19, 25, 35, and 40 mg/kg/d) without dietary cysteine. Intake levels were randomly assigned to each subject. Following enrollment, 2 subjects completed 2 intakes and 3 completed 3, while the remainder completed all 7. Mean TSAA requirement was determined from oxidation of L-[1-13C]phenylalanine using a mixed-effect change-point model. The 95% CI was calculated using parametric bootstrap. To test whether breakpoints were different between men and women, the overlap in the 95% CI was calculated. ResultsThe mean TSAA requirement was 26.2 (Rm2 = 0.39, Rc2 = 0.89; P < 0.001) and 17.1 mg/kg/d (Rm2 = 0.22, Rc2 = 0.79; P < 0.001) for men and women, respectively. The requirement was significantly higher in men than in women (difference in CI: 9.1 ± 8.85). ConclusionsTo our knowledge, this is the first study to determine the TSAA requirement in older adults. The requirement in older women is similar to current recommendations but is 75% higher in older men. These findings are important given recommendations for increased plant protein consumption. They will help in the assessment of diet quality and provide the basis of dietary guidelines for older adults consuming a plant-based diet.This trial was registered at clinicaltrials.gov as NCT04595188.

Higher adherence to the Dietary Approaches to Stop Hypertension (DASH Diet) is associated with lower greenhouse gases and land use from protein foods

IntroductionThe average American diet is high in red and processed meats which increases one's risk for chronic diseases and requires more land and water to produce and yields greater greenhouse gases (GHG) compared to other protein foods. Reduction of red and processed meat intake, such as seen with the Dietary Approaches to Stop Hypertension (DASH diet), could benefit human and environmental health.ObjectiveThe objective of this study is to predict the environmental sustainability of the DASH diet by evaluating the GHG, land use, and water withdrawals from protein foods within the self-selected diets of people who were encouraged to follow the DASH diet.MethodsDietary data was collected from 380 Midwesterners aged 35-70 years old with hypertension using the Automated Self-Administered 24-Hour (ASA 24) Recall System. DASH diet adherence was measured using a nutrient-based DASH score. GHG, land use, and water withdrawals were obtained using Carnegie Mellon University's Economic Input-Output Life Cycle Assessment (eiolca.net) using the Purchaser model (cradle-to-consumer). Multiple linear regressions were used to view associations between individual DASH nutrient scores and environmental impacts of total, animal, and plant protein foods.ResultsDiets that met DASH diet guidelines, as indicated by higher individual DASH nutrient scores, were associated with less GHG and land use from total and animal protein foods but more GHG and land use from plant-protein foods, with a few exceptions. The pattern was not clear for water withdrawals. Diets with the greatest adherence had around 25–50% lower GHG and land use from total protein foods than diets with the lowest adherence. Changes may be due to decreased consumption of total and animal protein foods, selection of animal protein foods with lower environmental impacts, and increased consumption of plant protein foods.ConclusionAdhering to the DASH diet can promote the consumption of less environmentally demanding protein foods resulting in lower GHG and land use from protein foods. However, claims regarding the sustainability of the entire dietary pattern cannot be determined based off the current study. Regardless, it is evident that environmental impacts should be considered alongside health impacts when selecting, promoting, or recommending a dietary pattern.