Apple Consumption Research Articles

Effect of sugars and non-nutritive sugar substitutes on consumption of apple leaves by codling moth neonates

Recently we reported that monosodium glutamate stimulates feeding in neonates of the codling mothCydia pomonella (L.). Herein we extend our general knowledge about feeding stimulators in neonates of this species, by presenting the effects of several sugars (sucrose, glucose, fructose, and maltose) and non-nutritive sugar substitutes (Sweet’n Low® and Equal®) on consumption of apple leaf (HoneycrispTM) tissue. Glucose, fructose, maltose and aspartame-based Equal had no effect on leaf consumption. Sucrose at a high concentration significantly reduced leaf consumption and delayed commencement of feeding. Sweet’n Low at high concentrations significantly increased leaf consumption and accelerated the commencement of feeding. Saccharin hemicalcium salt was identified as an active ingredient of Sweet’n Low. At 500 ppm and 1000 ppm, saccharin hemicalcium salt increased leaf consumption and accelerated commencement of feeding. The practical aspects of our findings are discussed.

Distribution of conjugated and free phenols in fruits: antioxidant activity and cultivar variations.

Total and free phenolic contents of 16 commonly consumed fruits (comprising 9 apples, 4 pears, and one each of peach, plum, and kiwi fruit cultivars) were measured by Folin-Ciocalteu assay. Total phenol contents varied from 272 to 475 mg of CtE/100 g of fresh weight. Of the apple cultivars studied, Braeburn and Empire had the highest and lowest total phenol content, respectively. The apple cultivars ranked in the following decreasing order: Braeburn > Red Delicious > Crisp Pink > Granny Smith > Royal Gala > Bramley > Golden Delicious > Fuji > Empire. Among pear cultivars, the order was Forelle > Taylor's > Peckham's > Conference. Peach and plum equally had high contents. The percentage of conjugated phenolics ranged between 3 (Red Delicious) and 77% (Empire) of the total phenols. Comparison of different cultivars of the same fruit and between different fruits showed broad variations in both phenolic content and in vitro antioxidant activity; a weak correlation (R(2) = 0.58) was observed between the phenolic content of the fruits and the total antioxidant activity, as estimated by the FRAP assay. The calculated dietary intake of total, free, and conjugated phenols from average per capita consumption of apples and pears in different regions of the U.K. varied between 104-126, 53-64, and 51-62 mg of CtE/day, respectively.

Melhoramento do cajueiro-anão-precoce: avaliação da qualidade do pedúnculo e a heterose dos seus híbridos

O consumo de pedúnculo de caju "in natura" vem experimentando incremento no Brasil após o cultivo dos novos clones de cajueiro-anão-precoce lançados nos anos oitenta. Apesar desses avanços, o consumo ainda é reduzido, uma vez que não ultrapassa 1% das cerca de 1,5 milhão de toneladas anuais produzidas no Nordeste. Este baixo consumo deve-se a alguns fatores, como o teor de tanino (0,27% a 0,72%) existente nos tipos cultivados de A. occidentale L. Por isso, a Embrapa vem realizando pesquisa objetivando a melhoria dessa característica nos clones anão-precoce CP76 e CP09, via retrocruzamento, com a espécie A. microcarpum L. Neste trabalho, iniciado em 1999, avaliaram-se o tanino, a acidez total (ATT%), os sólidos solúveis totais (SST), a relação SST/ATT, o pH e a textura de pedúnculos dos genitores empregados nos retrocruzamentos e seus respectivos híbridos, com os seguintes resultados: a) confirmaram-se os baixos teores de tanino (0,14%) e de ATT (0,16%) no A. microcarpum em relação às médias dos clones CP76 e CP09 (0,33% para tanino e 0,26% para ATT); b) o A. microcarpum também foi superior quanto à relação SST/ATT (80,6%) e a textura do pedúnculo (10,75 N), sendo os valores dos clones de 47,9 e 6,9N, respectivamente; c) estes resultados confirmam a importância do A. microcarpum como pai doador no melhoramento genético; d) os genitores não diferiram quanto aos teores de sólidos solúveis totais (SST) e pH; e) os híbridos entre A. microcarpum e A. occidentale exibiram acentuado vigor híbrido com relação aos teores de tanino, acidez e textura, e ausência de vigor híbrido em relação aos valores do pH e de sólidos solúveis totais (SST); f) não houve efeito nos cruzamentos recíprocos, para a maioria das características estudadas, à exceção do tanino.

Lyophilized Apple Counteracts the Development of Hypercholesterolemia, Oxidative Stress, and Renal Dysfunction in Obese Zucker Rats

Apples may have selective effects on abnormalities associated with the plurimetabolic syndrome. Therefore, the effects of 20% lyophilized apple supplementation on plasma and tissue lipids and on protection against susceptibility to oxidative stress and renal dysfunction were investigated in Zucker lean (Fa/-) or obese (fa/fa) rats. The experimental diets were equilibrated for sugar supply, contained 0.25 g/100 g cholesterol and provided only one third of the vitamin E requirement. Obese Zucker rats were hypercholesterolemic with cholesterol accumulation in LDL and HDL fractions. The apple diet lowered plasma and LDL cholesterol (-22 and -70%, respectively, P < 0.01) in obese Zucker rats and, in parallel, reduced triglyceride accumulation in heart and liver. Zucker rats fed the apple diet also had a larger intestinal pool and greater fecal excretion of bile acids. The heart concentration and urinary excretion of malondialdehyde were reduced by apple consumption in obese Zucker rats, suggesting better protection against peroxidation. Glucosuria and proteinuria in obese Zucker rats were also suppressed by the apple diet. In conclusion, despite their moderate fiber content, apples improve substantially the lipid status and peroxidative parameters in obese Zucker rats, suggesting that other plant constituents such as polyphenols are involved in these effects.

Food safety and organic fruit demand in Italy: a survey

Reports the first results of a study on how increased awareness of food safety can influence consumer behaviour regarding specific products. Looks into consumer attitudes towards organic apples by means of a survey conducted among customers of large retail outlets located in Emilia‐Romagna (Italy). Analyses consumer attitudes toward organic food, studying the price/quantity/quality relationship for this type of product. Consumers were directly interviewed at retail outlets, A questionnaire consisting of the following four sections was used: fruit consumption; pesticide abatement; organic fruit and organic apple demand; and demographic profile of respondent. Presents the results of the preliminary test phase of the survey. The pre‐test results are quite encouraging and enable us to start the final investigation round. The survey will continue in three rounds over a 12‐month period, trying in this way to override the relatively high seasonality of apple consumption.

Dietary antioxidants and asthma in adults: population-based case-control study.

A protective role for dietary antioxidants in asthma has been proposed. However, epidemiological evidence to implicate antioxidant vitamins is weak, and data on the role of flavonoid-rich foods and antioxidant trace elements are lacking. We carried out a population-based case-control study in South London, UK, to investigate whether asthma is less common and less severe in adults who consume more dietary antioxidants. Participants were aged 16-50 yr and registered with 40 general practices. Asthma was defined by positive responses to a standard screening questionnaire in 1996, and complete information about usual diet was obtained by food frequency questionnaire from 607 cases and 864 controls in 1997. After controlling for potential confounding factors and total energy intake, apple consumption was negatively associated with asthma (odds ratio [OR] per increase in frequency group 0.89 [95% confidence interval [CI]: 0.82 to 0.97]; p = 0.006). Intake of selenium was also negatively associated with asthma (OR per quintile increase 0.84 [0.75 to 0.94]; p = 0.002). Red wine intake was negatively associated with asthma severity. The associations between apple and red wine consumption and asthma may indicate a protective effect of flavonoids. The findings for dietary selenium could have implications for health policy in Britain where intake has been declining.

High-Throughput Fluorescence Screening of Antioxidative Capacity in Human Serum

Diphenylhexatriene-labeled phosphatidylcholine and propionic acid have been established as selective fluorescence markers for the continuous determination of oxidation processes in the lipid and aqueous phases of unfractionated human serum. Oxidation of the respective fluorophores leads to a decrease in fluorescence intensity from which the time-dependent degradation of the marker molecule can be determined. The lag times preceding the propagation of oxidation are representative for the antioxidative capacity of the system, which may be influenced by exogenous factors, e.g., the antioxidants from the diet. Supplementation of human serum by quercetin, rutin, vitamin E, vitamin C, or total apple phenolics in vitro led to a decrease in oxidizability depending on the oxidation marker and the hydrophobicity of the antioxidant. Quercetin and vitamin E showed a higher in vitro capacity of protecting lipoproteins against oxidation. In contrast, rutin and vitamin C were more efficient as inhibitors in the aqueous phase. The same effect on serum was found after dietary consumption of apples. This result is in line with the known observation that intake of plant polyphenols leads to an increase in serum levels of hydrophilic antioxidants.

Model-based development and testing of advertising messages: a comparative study of two campaign proposals based on the MECCAS model and a conventional approach

Traditionally, the development of advertising messages has been based on ‘creative independence’, sometimes catalysed by inductively generated empirical data. Due to the recent intensified focus on advertising effectiveness, this state of affairs is beginning to change.The purpose of the study described in this article is to compare the development and effects of two campaign proposals, with the common aim of increasing the consumption of apples among young Danes (18 to 35 years of age). One of the proposals is the result of an inductive-creative process, while the other is based on the MECCAS model, i.e. means—ends based data collection employing the laddering method and subsequent use of the guidelines for message development formulated in MECCAS. The project was a joint venture of the Association of Danish Fruit Growers, Odense, Denmark, and the MAPP Centre, and was financed by EU funds.The comparison involved the efficiency of the managerial communication taking place in the message development process as well as target group communication effects. The managerial communication was studied by interviews with the involved advertising agency (Midtmarketing, Ikast, Denmark) and client staff. The target group communication was assessed by pre-testing the two campaign proposals (n = 500).The main finding of the managerial study was that the implementation of the MECCAS guidelines for message development led to better agency-client communication, which resulted in a more common understanding of the objective of the campaign. The pre-test showed that the MECCAS-based message compared to the conventionally developed message was perceived as more focused by the target group and stimulated better central processing.

Food Sources of Phytoestrogens and Breast Cancer Risk in Mexican Women

We analyzed the intake of selected foods that contain phytoestrogens in relation to breast cancer (BC) risk using data from a hospital-based case-control study performed in Mexico City from 1994 to 1995. A total of 198 women with BC, aged 21-79 years, were individually age matched to an identical number of women with no breast disease. By a direct interview, information on socioeconomic characteristics and diet was obtained. A semiquantitative questionnaire was used to estimate the frequency of consumption of 95 foods. The effect of selected foods that contain phytoestrogens on BC risk was estimated using logistic regression models. The adjusted odds ratio for the consumption of more than one slice of onion per day and BC was 0.27 (95% confidence interval = 0.16-0.47), with a statistically significant trend (p < 0.001). This protective effect remained after adjustment for known risk factors of BC. Among premenopausal women, there was also a protective and significant effect due to the intake of lettuce and spinach and nonsignificant protective effects for the consumption of apples and herbal tea. Additional studies aimed at evaluating the potential protective effect of particular phytoestrogens on BC risk are needed.

Diet, lung function, and lung function decline in a cohort of 2512 middle aged men

BACKGROUNDA prospective cohort study of 2512 Welshmen aged 45–59 living in Caerphilly in 1979–1983 was used to investigate associations between diet and lung function.METHODSAt baseline (phase I) and at five...

Apple Juice Consumption Reduces Plasma Low-Density Lipoprotein Oxidation in Healthy Men and Women

ABSTRACT Epidemiological studies show that consumption of fruits and vegetables is associated with beneficial effects on human health including reduced risk of coronary artery disease (CAD). Fruits and their juices contain phytochemicals that inhibit in vitro low-density lipoprotein (LDL) oxidation and may account, in part, for their protective effect. However, reports of in vivo antioxidant effects from fruit intake are limited. We conducted a human trial to examine the in vivo effect of consumption of apples (both whole and juice) in an unblinded, randomized, crossover design. Healthy men and women added 375 ml of unsupplemented apple juice or 340 g of cored whole apple to their daily diet for 6 weeks, then crossed over to the alternate product for 6 weeks. Blood samples were obtained at baseline and after each dietary period. Compliance was monitored via biweekly 5-day food records, bodyweight checks, and meetings with study personnel. There were no significant differences between groups in intake of dietary fat, cholesterol, total carbohydrate, sugar, or calories throughout the study. Dietary fiber intake increased by 22% with whole apple consumption. Body weight, fasting serum lipid concentration, and other lipoprotein parameters were unchanged. Apple juice consumption increased ex vivo copper (Cu(++))-mediated LDL oxidation lag time by 20% compared with baseline. Apples and apple juice both reduced conjugated diene formation. Moderate apple juice consumption provides in vivo antioxidant activity. In view of the current understanding of CAD, the observed effect on LDL might be associated with reduced CAD risk and supports the inclusion of apple juice in a healthy human diet.

A Two‐Stage Analysis of the Effectiveness of Promotion Programs for U.S. Apples

AbstractExport promotion programs are intended to help U.S. exporters enter, maintain, and expand foreign markets, but little is known of their success in achieving each of these goals. In this paper we attempt to determine the effectiveness of export promotion in increasing both U.S. market share and total import consumption using apple imports by Singapore and the United Kingdom as case studies. Estimates of a two‐stage Linear Expenditure System (LES)/Almost Ideal Demand System (AIDS) model show that promotion increases consumption of apples in both the United Kingdom and Singapore, but increases U.S. market share only in the United Kingdom. The results suggest that free‐riding on U.S. promotion can be significant.

Dietary flavonoids and the risk of lung cancer and other malignant neoplasms.

Flavonoids are effective antioxidants and, in theory, may provide protection against cancer, although direct human evidence of this is scarce. The relation between the intake of antioxidant flavonoids and subsequent risk of cancer was studied among 9,959 Finnish men and women aged 15-99 years and initially cancer free. Food consumption was estimated by the dietary history method, covering the total habitual diet during the previous year. During a follow-up in 1967-1991, 997 cancer cases and 151 lung cancer cases were diagnosed. An inverse association was observed between the intake of flavonoids and incidence of all sites of cancer combined. The sex- and age-adjusted relative risk of all sites of cancer combined between the highest and lowest quartiles of flavonoid intake was 0.80 (95% confidence interval 0.67-0.96). This association was mainly a result of lung cancer, which presented a corresponding relative risk of 0.54 (95% confidence interval 0.34-0.87). The association between flavonoid intake and lung cancer incidence was not due to the intake of antioxidant vitamins or other potential confounding factors, as adjustment for factors such as smoking and intakes of energy, vitamin E, vitamin C, and beta-carotene did not materially alter the results. The association was strongest in persons under 50 years of age and in nonsmokers with relative risks of 0.33 (95% confidence interval 0.15-0.77) and 0.13 (95% confidence interval 0.03-0.58), respectively. Of the major dietary flavonoid sources, the consumption of apples showed an inverse association with lung cancer incidence, with a relative risk of 0.42 (95% confidence interval 0.23-0.76) after adjustment for the intake of other fruits and vegetables. The results are in line with the hypothesis that flavonoid intake in some circumstances may be involved in the cancer process, resulting in lowered risks.

Evaluation of recombinant allergens Bet v 1 and Bet v 2 (profilin) by Pharmacia CAP System in patients with pollen-related allergy to birch and apple

Sixty-five patients presenting either rhinoconjunctivitis or asthma and sensitized to pollens of trees of the order Fagales were studied by the Pharmacia CAP system in order to assess specific IgE for the important birch pollen allergens Bet v 1 and Bet v 2. All 65 subjects reacted to at least one of the recombinant birch allergens: 43% to Bet v 1, 30.7% to Bet v 2, and 26% to both. Patients monosensitized to birch did not react to Bet v 2. Of patients with a history of oral allergy syndrome after eating apples, 16/28 (57%) reacted to Bet v 1; among 20 polysensitized subjects presenting oral allergy syndrome after consumption of apple, four reacted to Bet v 2 (20%). Among patients with IgE against both recombinant allergens, six (35.30%) presented symptoms of allergy after eating apples. Our results indicate that sensitization to Bet v 1 is specific for birch and apple allergies, whereas sensitization to Bet v 2 is common in polysensitized patients.

Exposure to Volatile D-Pulegone Alters Feeding Behavior in European Starlings

D-pulegone repels birds, but it is unclear if its volatile cues can stimulate avoidance behavior or whether direct contact is required for this response. We used European starlings (Sturnis vulgaris) in feeding tests to investigate the role of volatile cues in d-pulegone's repellency. Direct contact with d-pulegone (1.0% vol/vol) coating decreased apple consumption by 53% (P < 0.02). When direct contact was prevented by encapsulating d-pulegone (10.0% vol/vol) in HistoPrep® capsules (Fisher Scientific, Pittsburgh, Pa.) or applying the stimulus solution to the underside of the feeding cup lids, ingestion of test foods was inhibited. The inhibitory effect of the volatile cues of 100% d-pulegone on feed ingestion was increased following repeated exposures. These results show that d-pulegone volatiles induced avoidance behavior in European starlings although direct contact with the compound produced a stronger response.

Ortho ‐aminoacetophenone, a non‐lethal repellent: The effect of volatile cues vs. direct contact on avoidance behavior by rodents and birds

Taxonomic differences in responsiveness to chemosensory irritants are prevalent among avian and mammalian species and represent a major obstacle to the development of general vertebrate repellents. We evaluated the effect of ortho-aminoacetophenone (OAP), a potent avian repellent, on ingestive behavior of two rodent species, Prairie vole (Microtus ochrogaster), Deer Mouse (Peromyscus maniculatus Wagn.), and an avian species, European starling (Sturnus vulgarus L.) utilizing similar experimental conditions to facilitate inter-species comparisons. All three species avoided OAP-treated food. Apple consumption by voles was decreased from a baseline of theoretical zero% by OAP (0.01-10.0 ml liter -1 ), P < 0.00001, while mice avoided all but the lowest concentration of OAP (0.01-10 ml liter -1 ), P < 0.00001. A repellent should elicit avoidance behavior prior to the animal having physical contact with the commodity, ideally producing aversion via volatile cues rather than through direct contact. Therefore, we utilized two delivery methods for presentation of the test solutions to evaluate the repellency of OAP in the presence and absence of direct contact. Apple consumption by birds following exposure to OAP by either direct contact or volatile cues differed from a baseline of theoretical zero% consumption, P < 0.001. When birds had access to OAP through both direct and volatile exposures, reduction in apple consumption by European starlings was greater than observed following contact with the compounds volatile cues alone, P < 0.03. These findings argue against a major role for olfaction or naso-trigeminal chemoreception in avoidance of OAP-treated food. Instead, taste or oral trigeminal chemoreception appear to mediate responding.

Vegetables, fruits, and risk of colorectal cancer: a case-control study from Uruguay.

To examine whether vegetable and fruit intake modify colorectal cancer risk, a case-control study was conducted in Uruguay. Dietary patterns were assessed in detail (for cases before diagnosis or symptoms occurred) by use of a food frequency questionnaire on 61 food items, which allowed the calculation of total energy intake. Nutrient residuals were calculated through regression analysis. After adjustment for potential confounders (which included body mass index, total energy, and total alcohol intake), a reduction in risk for total vegetable intake, total fruit intake, and lettuce, apple, and banana consumption was observed. The strongest protection was observed for banana intake (odds ratio 0.28; 95% confidence level 0.16-0.50) for consumption in the third tertile compared with the first.

りんごの需要動向と輸入解禁の影響

The objective of this study is to investigate the impact of lifting the ban on import of fresh apples to Japan. Japan opened the domestic market of fresh apples for trade in 1971. However, for prevention of epidemics, the ban on import of fresh apples had been still effective up to 1993.During these years, Japanese consumers have come to prefer to large red apples with their sweet in proportion to their acidity. These apples, which have been treated as “high quality apples” in Japan, began to produce in 1970s, Until then, various types of high quality apples have been introduced to the market.The ban on import for reasons of the prevention of epidemics was removed to New Zealand in 1993 and to the United States in 1994. It is the urgent matter for us to estimate the effect of lifting the ban on import of fresh apples to Japan. At the same time, our experience of the apple consumption is required us to estimate the domestic demand for apples with taking account of their varieties and qualities.According to the result of the demand analysis, we got the fact that Japanese consumers have a tendency to distinguish among the characteristics of apples clearly. Therefore, if the very high quality apples like the upper class of Japanese “Fuji” are imported to Japan, the domestic market price of apples might fall down drastically. Furthermore, because the recently domestic supply amount of apples are nearly at their saturation level, the domestic apple farmers must suffer heavy damage from the imports.Fortunately for the domestic apple farmers, our first imports of fresh apples from New Zealand and the United States were not a menace to the domestic production. Almost all of the apples imported to Japan were relatively small and ranked as low qualities. The result of estimation implied quantitatively that the impact of lifting the ban on import of fresh apples was not so serious for the apple production in Japan.

Hepatic acylcoenzyme A:cholesterol acyltransferase activity is low in hamsters fed apples in addition to a standard diet.

The effect of consumption of apples as a supplement to a standard diet on hepatic acylcoenzyme A: cholesterol acyltransferase (ACAT) activity was investigated in adult golden hamsters (Mesocricetus auratus). The experimental diet was given for 2 months. IN response to the high-fruit intake, the level of cholesteryl esters was reduced in the liver, and hepatic ACAT activity, determined in vitro under various conditions of incubation, was lowered by about 30%. Moreover, plasma cholesterol was redistributed among the lipoproteins, with a decrease in the cholesterol transported in the ApoB-rich lipoproteins.

Life styles and blood pressure: The protective effect of apple-eating habits on high blood pressure in a high-salt population.

Prospective epidemiological studies of blood pressure in a high-salt population in northeastern Japan were investigated along with dietary habits such as miso soup, rice, apple, fish, milk and sake consumption as well as smoking habits. Blood pressures of the populations in 3 villages were determined once or twice a year by mass surveys from 1954, 1957 or 1958 through 1975. The means and transitions of the personal blood pressure were calculated by regression analysis of the data obtained during each entire period. The number of persons was 1127 males and 1369 females and the response rate was 98.7 percent. The average number of times of determination of blood pressure for a person was 12.9. Stepwise multiple regression analyses were run with the means and transitions of systolic and diastolic blood pressure as the dependent variables and the life styles of the population in 1958 as an independent variable based on data of persons whose blood pressures were determined 5 or more times during the entire period. According to the backward stepwise method this study confirmed the positive relationship of age and sake drinking and the negative relationship of apple eating habits to blood pressure.