This study investigated the behavior of piezoelectric, dielectric, and elastic constants and the crystallinity in wood cellulose by repeated measurements (n = 42) between 100°C and 220°C. There was an insignificant change in the piezoelectric constant during repeated measurements in this temperature range. On the other hand, thermal decomposition of the amorphous region contributed to the decreasing trend of dielectric and elastic constants, although only a small increase in the elastic constant was found at the time of the initial measurements. The increase in the repeated measurements in this temperature range resulted in an increase in the piezoelectric loss modulus constantd 25″, which is closely related to energy loss. Thed 25″ peak shifted to a higher temperature with increasing measurements, which might be due not to the increase in rigidity of the wood specimen but to the increase in total peak area, which was observed during the later measurements. At the same time, variations of piezoelectric loss modulusd 25″ ande 25″ at advanced stages of the measurements suggested damage and structural changes in the wood.