With the escalating significance of renewable energy, there exists a pressing demand for precise software and tools capable of designing and forecasting wind turbine blade service life. OpenFAST, TurbSim, and MLife are pivotal open-source wind turbine simulation tools that integrate aerodynamics, structural dynamics, wind simulation, and control systems. They enable accurate prediction of wind turbine performance. This study aims to leverage these software resources to model turbulent wind conditions using predefined basic parameters and then calculate fatigue and longevity of the wind turbine blade to determine service life. Particularly, MLife was employed for rose loading, enhancing the fidelity of the loading cycle simulation. Collaboration between the National Renewable Energy Laboratory and the research team played a crucial role in establishing a foundational baseline and implementing realistic input parameters for evaluating the ultimate load, determining damage equivalent loads (DEL), and projecting service life. In all simulations, a constant factor of safety (FOS) of 1.5 was consistently applied. Additionally, Finite Element Analysis (FEA) was employed to validate our numerical experiments, ensuring the accuracy of the obtained results by correlating simulation outcomes with real-world scenarios, further strengthening the credibility of the study.