Nasalance is an acoustic representation of perceived nasality with proven clinical and research utility. Its validity is contingent on appropriate speech sample sets and distinct normative databases based on known impact factors such as language and phonetic environment, but little is known about the potential effects of lexical tone on nasalance. Its use in international cross-linguistic studies necessitates definition and added considerations of speech sampling protocol. Part I. A methodological review was undertaken using PRISMA (2020), the Scottish Intercollegiate Guidelines Network (SIGN) Methodology Checklist for evaluating risk of bias, and calculation of effect sizes and/or visual displays using tables showing grouping of similar data for synthesis of findings. Part II. A pilot study explored the effect of lexical tone on nasalance in Cantonese, a lexical tone language. Part I. 17 studies met the inclusion criteria. In addressing possible confounders and minimizing risk of bias, 13 studies were assigned an overall quality rating of (+) acceptable and the remaining 4, a high rating (++). For the 9-word string, there was a (non-clinical) difference of 3-5% between Swedish and Brazilian-Portuguese, and a moderate effect size for age (d=0.49); for the consonant-vowel syllables set (CV set), clinical differences across languages were identified for adults, and between 5-10% for children and adolescents. Part II. The pilot study showed a significant effect of lexical tone on nasalance where nasalance for the high-level tone 1 was significantly higher than that for high-rising tone 2, low-rising tone 5, and low-falling tone 4. There is a need for further evidence from other languages, including tonal languages, to better define the evidence and speech methodology of international cross-linguistic nasalance studies.