Conservation Strategies Research Articles

Contrasting water use strategies to climate warming in white birch and larch in a boreal permafrost region.

The effects of rising atmospheric CO2 concentrations (Ca) with climate warming on intrinsic water-use efficiency (iWUE) and radial growth in boreal forests are still poorly understood. We measured tree-ring cellulose δ13C, δ18O, and tree-ring width in Larix dahurica (larch) and Betula platyphylla (white birch), and analyzed their relationships with climate variables in a boreal permafrost region of northeast China over past 68years covering a pre-warming period (1951-1984; base period) and a warm period (1985-2018; warm period). We found that white birch but not larch significantly increased their radial growth over the warm period. The increased iWUE in both species was mainly driven by elevated Ca but not climate warming. White birch but not larch showed significantly positive correlations between tree-ring δ13C, δ18O and summer maximum temperature as well as vapor pressure deficit in the warm period, suggesting a strong stomatal response in the broad-leaved birch to temperature changes. The climate warming-induced radial growth enhancement in white birch is primarily associated with a conservative water use strategy. In contrast, larch exhibits a profligate water use strategy. It implies an advantage for white birch over larch in the warming permafrost regions.

Genetic diversity and haplotype distribution patterns analysis of cytb and RAG2 sequences in Rana hanluica from southern China

Rana hanluica: an endemic amphibian of China, is found in the hills and mountains south of the Yangtze River. In this comprehensive study, we collected 162 samples from 14 different localities to delve into the genetic diversity of Rana hanluica using mitochondrial Cytb and nuclear RAG2 as genetic markers. Our findings reveal that the Nanling Mountains, specifically regions like Jiuyi Shan, Jinggang Shan, Mang Shan, and Qiyun Shan, are genetic hotspots harboring remarkable diversity. The research results also indicate that there is gene flow among the various populations of the species, and no distinct population structure has formed, which may be due to migration. Moreover, populations in some regions, as well as the overall population, show signs of a possible genetic bottleneck, which we speculate may have been caused by climate change. However, given the exploratory nature of our study, further investigations are warranted to confirm these observations. Through phylogenetic analyses, we uncovered indications that R. hanluica might have originated within the Nanling region, dispersing along the east-west mountain ranges, with a significant contribution originating from Jiuyi Shan. The genetic distributions uncovered through our research reflect historical migratory patterns, evident in the distinct haplotypes of the RAG2 gene between the western and eastern parts of the studied area. Moreover, Heng Shan and Yangming Shan exhibited unique genetic signatures, possibly influenced by geographic isolation, which has shaped their distinct genotypes. The insights gained from this study hold profound implications for conservation efforts. By identifying regions rich in genetic diversity and crucial gene flow corridors, we can develop more effective conservation strategies. Preserving these genetically diverse areas, especially within the Nanling Mountains, is vital for maintaining the evolutionary potential of R. hanluica. In conclusion, our research has laid a solid foundation for understanding the genetic landscape of R. hanluica, shedding light on its origins, population structures, and evolutionary trajectories. This knowledge will undoubtedly guide future research endeavors and inform conservation strategies for this endemic amphibian.

Small but connected islands can maintain populations and genetic diversity under climate change

In response to the striking effects of environmental change, conservation strategies often include the identification of conservation areas that can effectively maintain vulnerable species. Consequently, identifying system‐specific conditions that maintain the demographic and genetic viability of species of conservation concern is essential. Connectivity plays a critical role in the persistence of populations. Islands have been model systems to understand connectivity and metapopulation processes and have emerged as particularly favorable targets for conservation. While islands can be isolated from mainland disturbances, it is unknown what degree of isolation is necessary to avoid unfavorable changes but remain sufficiently connected to maintain population viability. To test this question, we explored connectivity within the Apostle Islands, an archipelago of 22 islands within Lake Superior, by comparing historical and contemporary trends in ice bridge connectivity and by simulating the effect of reduced connectivity within this system. We developed a demographically informed individual‐based model to explicitly test the role of connectivity to influence the persistence and genetic diversity of American marten Martes americana, a forest carnivore at risk across its southern range boundary. We found that genetic diversity was resilient to moderate changes in ice cover, but a complete loss of connectivity resulted in rapid genetic erosion. Despite genetic erosion, populations persisted as long as nominal connectivity occurred between islands. Our work suggests that connectivity will decline, but martens would be resilient to moderate changes and, in the short term, the Apostle Islands can act as a refuge along this species' southern range boundary. Identifying thresholds in connectivity that maintain populations but allow for isolation from disturbance will be necessary to identify suitable areas for species conservation across space and time.

Rangelands and Pastoralism in Globalized Economies: Policy Paralysis and Legal Requisites

Growing quest for globalization and expanding economies have resulted into fragmentation, enclosure, grabbing, militarization and devastation of rangelands. Grasslands – covering 70% of the global agricultural area – are the basis for livestock production. In most of the countries, governments have little recognition of communal tenures of agro-pastoralists. Consequently, both pastoralists and rangeland ecosystems have suffered a grim fate. On the contrary, the subsistence pastoralism is an established sustainable strategy of livelihood and ecosystem conservation in the rangelands. Unfortunately, some of the most nutritive foods and other sustainable products of nomadic pastoralists have not desirably been priced in modern markets. With the demonstrated cases exhibiting the nomadic pastoralists, such as Hutsul shepherd communities of Ukraine, as most sustainable societies on planet Earth, there is urgent need for reshaping the popular paradigm and State policies on rangeland commons. In isolation of pastoralist people, the rangelands cannot truly be conserved or protected. To begin with, the resilience of pastoralists to the changing environments and their (unique) rangeland management can first be pondered. Accordingly, the policy and legal frameworks of States need to be reoriented and revised. In particular, Eurasian countries should review their laws and policies on rangeland sustainability and pastoral grazing.

Density Mediates the Predator-Induced Growth and Metamorphic Plasticity of Chinhai Spiny Newt Larvae

Predators significantly influence amphibian larval development. Predator-induced plasticity is often studied independently from conspecific density effects, but these environmental factors may interact. We conducted two-factor factorial experimental design to manipulate conspecific density and predator cues, aiming to investigate the independently or interactive impacts of these two factors on the development of Chinhai spiny newt larvae (Echinotriton chinhaiensis). Our findings reveal that both high and low conspecific densities constrain spiny newt larval growth and predators also limit growth. Interestingly, high conspecific density restricts predator-induced growth plasticity without interacting effects. Only lower density groups exhibit slower growth responses to predators. Our study investigates how density mediates predator-induced plasticity in the endangered Chinhai spiny newt larvae, providing insights into their intricate life history. These results contribute to the understanding of predator-induced plasticity in amphibians and provide insights into the adaptive strategies of endangered species like Chinhai spiny newt. Such knowledge informs the development of effective conservation strategies for their protection.

A vulnerability evaluation method of earthen sites based on entropy weight-TOPSIS and K-means clustering

The degradation of earthen sites due to natural and human factors has become a pressing issue, necessitating urgent protection measures. In this context, accurate assessment of the vulnerability of earthen sites is essential for the development of effective conservation strategies. In this study, a comprehensive evaluation framework that incorporates multiple indicators is proposed. In particular, the entropy weight- (Technique for Order Preference by Similarity to an Ideal Solution) TOPSIS method is employed for quantitative vulnerability assessment and combined with K-means clustering to define vulnerability levels for earthen sites. To validate the proposed approach, the vulnerability of 29 sections of the Ming Great Wall is evaluated. Eventually, the 29 earthen sites are categorized into three levels: high, medium, and low, according to their degree of vulnerability. The results of gray correlation analysis and entropy weight-TOPSIS method are compared using the ontology missing amount in the original data as the validation standard. The results show that the Pearson correlation coefficient value of the entropy weight-TOPSIS method with the ontology missing amount was 0.859, while the Pearson correlation coefficient value of the gray correlation analysis method with the ontology missing amount was 0.691, so that the results of the entropy weight-TOPSIS method can more accurately reflect the actual vulnerability of earthen sites.

Management of osteonecrosis of the humeral head in the pediatric population: A systematic review

Purpose: Humeral head osteonecrosis in the pediatric patients most often occurs in patients with underlying hemoglobinopathies, exposure to chronic corticosteroids, or after trauma. The purpose of this study was to perform a systematic review evaluating the prevalence, clinical characteristics, and management of humeral head osteonecrosis in the pediatric population. Methods: PubMed, Ovid MEDLINE, and Scopus were screened with the terms “osteonecrosis,” “avascular necrosis,” “pediatric,” and “proximal humerus” on January 10, 2024. A total of 218 studies were screened, and 74 studies were evaluated for eligibility. Studies that reported on the prevalence and/or management of pediatric humeral head osteonecrosis were included. The systematic review was conducted according to the Preferred Reporting Items for Systematic Review and Meta-Analyses guidelines. Results: Twelve studies met inclusion criteria: four retrospective case series, three prospective case series, one retrospective cohort study, one retrospective case-control study, and three case reports. A majority of the studies (67%) discussed chemotherapy-induced osteonecrosis of the humeral head. A total of 77 patients (106 shoulders) with humeral head osteonecrosis were identified. The overall prevalence of osteonecrosis of the humeral head across eight studies examining at-risk populations (underlying hemoglobinopathies or undergoing chemotherapy) was 2%. Intra-articular steroid injections, physical therapy, and activity modification are effective conservative management strategies. Additionally, core decompression and hemiarthroplasty are surgical treatment options. Conclusions: The prevalence of osteonecrosis of the humeral head is low even among at-risk populations with associated medical conditions. A variety of conservative and surgical treatment options have been described, but no comparative evaluations of these modalities has been conducted. Level of evidence: IV.

Integrating Life Cycle Cost Analysis for Sustainable Maintenance of Historic Buildings

This study examines the strategic use of life cycle cost analyses (LCCAs) in the management and conservation of heritage sites, emphasizing the need for comprehensive financial planning. With an increasing number of heritage sites showing signs of deterioration, our aim was to improve the sustainability and effectiveness of restoration practices. We used dynamic life cycle costing methods and developed the MONUREV software V2 to simulate different restoration scenarios, providing accurate, data-driven projections for maintaining structural, functional and aesthetic integrity. The field research involved testing these methods through case studies of heritage buildings in the Czech Republic, focusing on holistic cost management from initial analysis to practical application. The results showed that LCC analysis can significantly assist in making informed decisions, balancing economic and cultural values, and ensuring long-term conservation outcomes. This study concludes that the integration of a detailed LCC analysis into heritage conservation strategies represents a methodological advance that can significantly improve the economic and operational planning of the maintenance of heritage buildings, thereby ensuring their preservation for future generations.

Niche structure and habitat shifts for coastal sharks of the US Southeast Atlantic and Gulf of Mexico

AbstractUnderstanding spatial ecology and predicting animal movements in response to environmental changes, such as anthropogenic climate change and multidecadal variability, is critical for effective conservation strategies. Niche structuring is key to some coastal shark species and size classes coexisting in the US Atlantic and Gulf of Mexico to limit interspecific and intraspecific interaction. Data from four fishery‐independent bottom longline surveys were used to evaluate the abiotic ecological niches of eight species of small and large coastal sharks. Gaussian mixture models separated length composition data into 14 size categories for ecological niche analysis. Generalized additive mixed effect models were fit and coupled with output from dynamic high‐resolution ocean models to predict suitable abiotic habitats, evaluate potential shifts in distribution, and explore the impacts of large‐scale climatological trends on abiotic habitats from 1994 to 2019. The abiotic niche for small coastal sharks generally tended toward warmer, high salinity, shallow bottom waters close to shore. No overarching niche was found for large coastal sharks, but appreciable ontogenetic differences were seen. Most taxa analyzed exhibited declining annual trends in higher quality habitats, particularly during fall months. The analysis provided evidence of shifts north along the Atlantic, to deeper offshore waters in the Atlantic and Gulf of Mexico, and the potential to redistribute in response to multidecadal climate variability for multiple species. The analytical framework described could aid in developing various spatiotemporal management measures, and results provide insight into the habitat characteristics of several species over broad spatiotemporal ranges and through ontogeny.

Geoinformatics-Based Mapping of Environmental Sensitive Areas for Desertification over Satara and Sangli Districts of Maharashtra, India

Desertification processes in arid, semi-arid, and dry sub-humid conditions have been enhanced in recent decades. The geospatial database and associated satellite data can be effectively employed for regional planning to address desertification and land degradation. In this study, the Mediterranean Desertification and Land Use (MEDALUS) model has been used to map environmentally sensitive areas due to desertification in the Satara and Sangli districts of Maharashtra, India. This was achieved by combining Landsat-8 multispectral data, Census data, soil data, and climatic variables like temperature, rainfall, and evapotranspiration. The algorithm of MEDALUS is the geometric mean of four indicators, namely soil quality index (SQI), climate quality index (CQI), vegetation quality index (VQI), and socio-economic quality index (SEQI). The findings indicated that the majority of the study area comes under the potential category of desertification (60.32%) followed by fragile (27.87%) and critical (11.81%). Areas with a high propensity for desertification were found over the low to very low climatic quality and moderate to high soil quality including lower socio-economic quality. The lower socio-economic quality is mainly due to high to very high population density (>100 people/km2), low to moderate illiteracy rate (<16%), and low to moderate work participation rate (<50%) that incentivize unsustainable land use practices. The study provides a valuable tool for understanding and managing natural resources. It offers a detailed analysis of the environmental sensitivity of the study area, taking into account various factors like land use, vegetation cover, slope, and soil erosion potential. The developed comprehensive map of the area helps in identifying the most sensitive regions and developing appropriate conservation strategies. The information obtained from the study can be utilized to develop and implement successful measures to prevent or alleviate desertification, which is crucial for sustaining the health of ecosystems and the welfare of local residents.

Land use intensification causes the spatial contraction of woody-plant based ecosystem services in southwestern Ethiopia

Integrating biodiversity conservation and food production is vital, particularly in the tropics where many landscapes are highly biodiverse, and where people directly depend on local ecosystems services that are linked to woody vegetation. Thus, it is important to understand how woody vegetation and the benefits associated with it could change under different land-use scenarios. Using a comprehensive, interdisciplinary study in southwestern Ethiopia, we modeled current and future availability of woody plant-based ecosystem services under four scenarios of landscape change. Land-use scenarios with intensified food or cash crop cultivation would lead to the contraction of woody-plant based ecosystem services from farmland to forest patches, increasing pressure on remaining forest patches. This raises questions about the viability of conventional intensification combined with land sparing—where conservation and production are separated—as a viable strategy for conservation in tropical landscapes where woody-plant based ecosystem services are vital to the lives of local communities.

Priority effects alter microbiome composition and increase abundance of probiotic taxa in treefrog tadpoles.

Harnessing the functions of host-associated bacteria is a promising mechanism for managing disease outcomes across different host species. In the case of amphibians, certain frog-associated bacteria can mitigate lethal outcomes of infection by the fungal pathogen Batrachochytrium dendrobatidis. Successful probiotic applications require knowledge of community assembly and an understanding of the ecological mechanisms that structure these symbiotic bacterial communities. In our study, we show the importance of environment and parentage in determining bacterial community composition and that community composition can be influenced by priority effects. Further, we provide support for the use of bacterial priority effects as a mechanism to increase the relative abundance of target probiotic taxa in a developing host. While our results show that priority effects are not universally effective across all host-associated bacteria, our ability to increase the relative abundance of specific probiotic taxa may enhance conservation strategies that rely on captive rearing of endangered vertebrates.

Genome-wide detections for runs of homozygosity and selective signatures reveal novel candidate genes under domestication in chickens

BackgroundIndigenous chickens were developed through a combination of natural and artificial selection; essentially, changes in genomes led to the formation of these modern breeds via admixture events. However, their confusing genetic backgrounds include a genomic footprint regulating complex traits, which is not conducive to modern animal breeding.ResultsTo better evaluate the candidate regions under domestication in indigenous chickens, we considered both runs of homozygosity (ROHs) and selective signatures in 13 indigenous chickens. The genomes of Silkie feather chickens presented the highest heterozygosity, whereas the highest inbreeding status and ROH number were found in Luhua chickens. Short ROH (< 1 Mb), were the principal type in all chickens. A total of 291 ROH islands were detected, and QTLdb mapping results indicated that body weight and carcass traits were the most important traits. An ROH on chromosome 2 covering VSTM2A gene was detected in 12 populations. Combined analysis with the Tajima’s D index revealed that 18 genes (e.g., VSTM2A, BBOX1, and RYR2) were under selection and covered by ROH islands. Transcriptional analysis results showed that RYR2 and BBOX1 were specifically expressed in the heart and muscle tissue, respectively.ConclusionBased on genome-wide scanning for ROH and selective signatures, we evaluated the genomic characteristics and detected significant candidate genes covered by ROH islands and selective signatures. The findings in this study facilitated the understanding of genetic diversity and provided valuable insights for chicken breeding and conservation strategies.

The Association between Rapid Urbanization Process and Mangrove Land Use Changes in Coastal Regions of Mainland China: A ten-years Systematic Review

Objective: This research aims to summarize the impacts on mangroves in mainland China due to changes in land use patterns in the context of rapid urbanization. Theoretical Framework: Grounded in theories of urbanization, land use change, and ecosystem dynamics, this research adopts a theoretical framework that integrates concepts from geography, ecology, and urban studies. It explores the complex interactions between urban development and mangrove ecosystems, considering factors such as population growth, economic development, and policy interventions. Method: The systematic literature review method used in this study follows the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. From 359 papers, 14 were selected for review and summary based on sources and identification, inclusion and exclusion criteria, screening, and data extraction. Results and Discussion: We realize that development activities that land use, such as land reclamation and port construction, pose the most significant threat to mangroves, often leading to irreversible damage. In addition to the obvious destruction, the covert and sustained impacts of urban development on mangrove forests have been receiving increasing attention. To address the negative impact of substantial demand for construction land, the establishment of protected areas at different levels has been proven to be indeed effective. However, the sustainable development of management and service functions within protected areas requires further research. Research Implications: Understanding the specific impacts of the urbanization process on mangrove forests is crucial for mangrove restoration efforts and for formulating and adjusting urban development-related plans and policies. Originality/Value: This study assesses urbanization impacts on mangroves, detailing loss reasons and distinguishing explicit and implicit effects of construction. It evaluates protective measures and discusses tailored conservation strategies for sustainable mangrove management.

Climate change effects on animal presence in the Massaciuccoli Lake basin

Big-data mining approaches based on Artificial Intelligence models can help forecast biodiversity changes before they happen. These approaches can predict macroscopic species distribution patterns and trends that can inform preventive measures to avoid the loss of ecosystem functions and services. They can, therefore, help study and mitigate climate change implications on biodiversity conservation in fragile ecosystems. Wetlands are particularly fragile ecosystems where climate change poses severe risks and has dramatically reduced their size over the past century, with profound consequences on biodiversity and ecosystem services. Through big-data mining approaches, we can predict future wetland biodiversity trends in the context of climate change. This paper proposes such predictive analysis for a specific wetland: The Massaciuccoli Lake basin in Tuscany, Italy. This basin is a critical tourist attraction due to its rich biodiversity, making it an area of interest for citizens, tourists, and scientists. However, the region's suitability for native and non-native species is at risk due to climate and land-use change. Using machine-learning models, we predict the potential effects of climate change on animal spatial distribution in the basin under different greenhouse gas emission scenarios. The results suggest that habitat suitability has generally improved from 1950 to today, presumably owing to the targeted conservation strategies adopted in the area, but climate change will severely reduce bird biodiversity by 2050 while favouring several insect species' proliferation and other species' habitat change, even under a medium-emission scenario. This will lead to significant changes in the basin's biodiversity. Our methodology is adaptable to other wetland basins, being fully based on open data and models. The spatially explicit modelling used in this research provides valuable information for policymakers and spatial planners, complementing traditional biodiversity trend analyses.

Recent changes in tropical-dry-forest connectivity within the Balsas Basin Biogeographic Province: potential effects on endemic-bird distributions

Maintaining landscape connectivity is a conservation priority for biodiversity as it may mitigate the adverse effects of forest degradation, fragmentation, and climate change by facilitating species dispersal. Despite their importance for biodiversity conservation, Mexican tropical dry forests (TDFs) face high fragmentation rates due to anthropogenic activities. In this study, we analyzed the connectivity dynamics of TDFs in the Balsas Basin Biogeographic Province (BBBP) between 2013 and 2018, focusing on old-growth and secondary TDF covers, including Protected Areas and Important Bird Areas. We evaluated the effects of connectivity loss and gain on the distribution areas of 30 endemic bird species with ecological associations with TDFs in the BBBP. We found expansion in TDFs accounting for a total increase of 227,905 ha due to secondary forest increase (12%). In contrast, old-growth forests experienced a reduction of 66,576 ha in the study area (8%). We also found a decrease in areas with high and very-high connectivity, coupled with an increase in low connectivity, except for TDFs inside Protected Areas, which increased by 3,000 ha, leading to higher connectivity. There was an increase in total forest cover in 27 species’ potential distribution, highlighting the possible role of secondary forests in promoting connectivity between old-growth forest patches. Our results reveal the complex dynamics between forest types, connectivity, and bird-species distributions. Despite an overall increase in forested areas, most TDFs continue to have low connectivity, likely impacting biodiversity, particularly for species that rely on highly conserved ecosystems. This study underscores the importance of integrated conservation strategies considering connectivity, forest recovery, and the dynamics of species-ecosystem interactions.

Management of the Devastated Bladder Outlet after Prostate CANCER Treatment.

Devastating complications of the bladder outlet resulting from prostate cancer treatments are relatively uncommon. However, the combination of the high incidence of prostate cancer and patient longevity after treatment have raised awareness of adverse outcomes deteriorating patients' quality of life. This narrative review discusses the diagnostic work-up and management options for bladder outlet obstruction resulting from prostate cancer treatments, including those that require urinary diversion. The devastated bladder outlet can be a consequence of the treatment of benign conditions, but more frequently from complications of pelvic cancer treatments. Regardless of etiology, the initial treatment ladder involves endoluminal options such as dilation and direct vision internal urethrotomy, with or without intralesional injection of anti-fibrotic agents. If these conservative strategies fail, surgical reconstruction should be considered. Although surgical reconstruction provides the best prospect of durable success, reconstructive procedures are also associated with serious complications. In the worst circumstances, such as prior radiotherapy, failed reconstruction, devastated bladder outlet with end-stage bladders, or patient's severe comorbidities, reconstruction may neither be realistic nor justified. Urinary diversion with or without cystectomy may be the best option for these patients. Thorough patient counseling before treatment selection is of utmost importance. Outcomes and repercussions on quality of life vary extensively with management options. Meticulous preoperative diagnostic evaluation is paramount in selecting the right treatment strategy for each individual patient. The risk of bladder outlet obstruction, and its severest form, devastated bladder outlet, after treatment of prostate cancer is not negligible, especially following radiation. Management includes endoluminal treatment, open or robot-assisted laparoscopic reconstruction, and urinary diversion in the worst circumstances, with varying success rates.

Influence of resource gradients and habitat edges on density variation in tiger populations in a multi-use landscape

Spatial heterogeneity in the local densities of terrestrial carnivores is driven by multiple interacting biotic and abiotic factors. Space-use patterns of large carnivores reflect the competing demands of resource selection (e.g., exploitation of habitats with abundant prey) and minimization of risks arising from human interactions. Estimating the relative strength of these drivers is essential to understand spatial variation in densities of large carnivores and there are still key knowledge gaps for many large carnivore populations. To better understand the relative roles of environmental and human drivers of spatial variation in tiger (Panthera tigris) densities, we surveyed a 3000 km2 landscape in North India using camera trap data. Over two years, we photo-captured 92 unique adult tigers. Associating spatial covariates with patterns of detection allowed us to test hypotheses about the relative influence of prey abundance, habitat structure and extent, and proximity to habitat edges on spatial variation in tiger densities across a gradient of anthropogenic disturbance. We documented extensive variation in tiger density within and across management units and protected areas. Spatial variation in prey abundance and proximity to grassland habitats, rather than human use (e.g. extent of human-dominated edge habitat and protection status), explained most of the spatial variation in tiger density in two of the five surveyed sites. The region’s largest tiger population occurred in a multi-use forest beyond protected area boundaries, where wild ungulates were abundant. Our results suggest that tigers can occur at high densities in areas with extensive human use, provided sufficiently high prey densities, and tracts of refuge habitats (eg. areas with dense vegetation with low human use). We argue that tiger conservation portfolio can be expanded across multi-use landscapes with a focus on areas that are adaptively managed as “zones of coexistence” and “refuge habitats”. Advancing this conservation strategy is contingent on greatly strengthening systems to effectively and equitably redress human–wildlife conflict and leveraging existing policies to strengthen local participation in conservation planning and forest stewardship. Our insights into the environmental drivers of spatial heterogeneity in tiger populations can inform both local management and guide to species recovery in working landscapes.

Safety and efficacy of active blood-pressure reduction to the recommended thresholds for intravenous thrombolysis in patients with acute ischaemic stroke in the Netherlands (TRUTH): a prospective, observational, cluster-based, parallel-group study.

Intravenous thrombolysis is contraindicated in patients with ischaemic stroke with blood pressure higher than 185/110 mm Hg. Prevailing guidelines recommend to actively lower blood pressure with intravenous antihypertensive agents to allow for thrombolysis; however, there is no robust evidence for this strategy. Because rapid declines in blood pressure can also adversely affect clinical outcomes, several Dutch stroke centres use a conservative strategy that does not involve the reduction of blood pressure. We aimed to compare the clinical outcomes of both strategies. Thrombolysis and Uncontrolled Hypertension (TRUTH) was a prospective, observational, cluster-based, parallel-group study conducted across 37 stroke centres in the Netherlands. Participating centres had to strictly adhere to an active blood-pressure-lowering strategy or to a non-lowering strategy. Eligible participants were adults (≥18 years) with ischaemic stroke who had blood pressure higher than 185/110 mm Hg but were otherwise eligible for intravenous thrombolysis. The primary outcome was functional status at 90 days, measured using the modified Rankin Scale and assessed through telephone interviews by trained research nurses. Secondary outcomes were symptomatic intracranial haemorrhage, the proportion of patients treated with intravenous thrombolysis, and door-to-needle time. All ordinal logistic regression analyses were adjusted for age, sex, stroke severity, endovascular thrombectomy, and baseline imbalances as fixed-effect variables and centre as a random-effect variable to account for the clustered design. Analyses were done according to the intention-to-treat principle, whereby all patients were analysed according to the treatment strategy of the participating centre at which they were treated. Recruitment began on Jan 1, 2015, and was prematurely halted because of a declining inclusion rate and insufficient funding on Jan 5, 2022. Between these dates, we recruited 853 patients from 27 centres that followed an active blood-pressure-lowering strategy and 199 patients from ten centres that followed a non-lowering strategy. Baseline characteristics of participants from the two groups were similar. The 90-day mRS score was missing for 15 patients. The adjusted odds ratio (aOR) for a shift towards a worse 90-day functional outcome was 1·27 (95% CI 0·96-1·68) for active blood-pressure reduction compared with no active blood-pressure reduction. 798 (94%) of 853 patients in the active blood-pressure-lowering group were treated with intravenous thrombolysis, with a median door-to-needle time of 35 min (IQR 25-52), compared with 104 (52%) of 199 patients treated in the non-lowering group with a median time of 47 min (29-78). 42 (5%) of 852 patients in the active blood-pressure-lowering group had a symptomatic intracranial haemorrhage compared with six (3%) of 199 of those in the non-lowering group (aOR 1·28 [95% CI 0·62-2·62]). Insufficient evidence was available to establish a difference between an active blood-pressure-lowering strategy-in which antihypertensive agents were administered to reduce blood pressure below 185/110 mm Hg-and a non-lowering strategy for the functional outcomes of patients with ischaemic stroke, despite higher intravenous thrombolysis rates and shorter door-to-needle times among those in the active blood-pressure-lowering group. Randomised controlled trials are needed to inform the use of an active blood-pressure-lowering strategy. Fonds NutsOhra.

Population Status of the Endangered Semi-Mangrove Dolichandrone spathacea on Hainan Island, China

In China, Dolichandrone spathacea is a rare and endangered semi-mangrove plant species with an extremely small population, naturally distributed only in Zhanjiang City and the east coast of Hainan Island. Despite conservation concerns, the population status of D. spathacea has received little scientific attention. In this study, we evaluated the current status of D. spathacea on Hainan Island, China, in order to propose sustainable conservation strategies for future ecological restoration of its natural population. D. spathacea on Hainan Island can be divided into four populations. All the D. spathacea populations present a state of overall dispersion, local concentration, and occasionally sporadic existence, and they exist in geographical isolation. The young, middle, and old D. spathacea plants account for 20.42%, 66.20%, and 13.38%, respectively, indicating that the D. spathacea population on Hainan Island is declining. Furthermore, instead of temporal structure, we used diameter at breast height (DBH) to establish a static life table, draw a population survival curve, and quantify the future development trend through population dynamic analysis and time-series prediction. These results suggest that the D. spathacea population in the Bamen Gulf (Wenchang) and Qingmei Harbor (Sanya) on Hainan Island is sensitive to external disturbances and possesses two main increases in mortality rate—namely, in its juvenile and mature stages—due to competition and anthropogenic interferences, which might be the most important reasons for its endangered status. Depending on the current conditions of the D. spathacea population, we should conserve and expand mature trees in situ, preserve their germplasm resources, rehabilitate their habitats to promote provenance restoration, and conduct artificial cultivation and spreading planting in order to realize the sustainable conservation and management of D. spathacea.