Conservation Of Animal Genetic Resources Research Articles

Genetic structure of cattle of the siberian branch by microsatellite loci

Molecular-genetic methods are essential tools for the utilization and conservation of animal genetic resources. These methods facilitate more efficient management and control of breeding programs within livestock production systems. For studying the genetic diversity of a population, the use of STR markers is relevant due to the high variability of repeats. This study presents a genetic characterization of a Holstein and Black Pied cattle population (n = 10233) in Western Siberia using 12 microsatellite loci (BM1818, BM1824, BM2113, ETH10, ETH225, ETH3, INRA023, SPS115, TGLA126, TGLA122, TGLA227, TGLA53). A total of 145 alleles were identified across all loci, with frequencies ranging from 0.00005 to 0.68961. The highest level of genetic diversity was observed at the TGLA122 locus (25 alleles) with an average number of effective alleles (Ne) of 4.5. The least polymorphic locus was BM1824 (7 alleles) with an average Ne of 3.27. The average observed (Ho) and expected (He) heterozygosity across all loci was 0.6. The highest variability was observed at the TGLA53 locus, with a Wright’s fixation index (Fis) of 0.161, indicating a heterozygote deficiency. A similar deficiency was observed at the BM1818 locus. All other loci exhibited a positive Fis, with the highest value observed at the ETH3 locus (-0.074), indicating an excess of heterozygotes. The average Fis across all loci was -0.02, suggesting a sufficient level of heterozygosity within the studied population. These findings provide valuable information for population studies and practical breeding programs aiming to manage genetic diversity and improve selection efficiency in this cattle population.

Population structure and breed identification of Chinese indigenous sheep breeds using whole genome SNPs and InDels

BackgroundAccurate breed identification is essential for the conservation and sustainable use of indigenous farm animal genetic resources. In this study, we evaluated the phylogenetic relationships and genomic breed compositions of 13 sheep breeds using SNP and InDel data from whole genome sequencing. The breeds included 11 Chinese indigenous and 2 foreign commercial breeds. We compared different strategies for breed identification with respect to different marker types, i.e. SNPs, InDels, and a combination of SNPs and InDels (named SIs), different breed-informative marker detection methods, and different machine learning classification methods.ResultsUsing WGS-based SNPs and InDels, we revealed the phylogenetic relationships between 11 Chinese indigenous and two foreign sheep breeds and quantified their purities through estimated genomic breed compositions. We found that the optimal strategy for identifying these breeds was the combination of DFI_union for breed-informative marker detection, which integrated the methods of Delta, Pairwise Wright's FST, and Informativeness for Assignment (namely DFI) by merging the breed-informative markers derived from the three methods, and KSR for breed assignment, which integrated the methods of K-Nearest Neighbor, Support Vector Machine, and Random Forest (namely KSR) by intersecting their results. Using SI markers improved the identification accuracy compared to using SNPs or InDels alone. We achieved accuracies over 97.5% when using at least the 1000 most breed-informative (MBI) SI markers and even 100% when using 5000 SI markers.ConclusionsOur results provide not only an important foundation for conservation of these Chinese local sheep breeds, but also general approaches for breed identification of indigenous farm animal breeds.

Classification accuracy of machine learning algorithms for Chinese local cattle breeds using genomic markers.

Accurate breed classification is required for the conservation and utilization of farm animal genetic resources. Traditional classification methods mainly rely on phenotypic characterization. However, it is difficult to distinguish between the highly similar breeds due to the challenges in qualifying the phenotypic character. Machine learning algorithms show unique advantages in breed classification using genomic information. To evaluate the classification methods for Chinese cattle breeds, this study utilized genomic SNP data from 213 individuals across seven Chinese local breeds and compared the classification accuracies of three feature selection methods (FST value sorting and screening, mRMR, and Relief-F) and three machine learning algorithms (Random Forest, Support Vector Machine, and Naive Bayes). Results showed that: 1) using the FST method to screen more than 1500 SNPs, or using the mRMR algorithm to screen more than 1000 SNPs, the SVM classification algorithm can achieve more than 99.47% classification accuracy; 2) the most effective algorithm was SVM, followed by NB, while the best SNP selection method was FST and mRMR, followed by Relief-F; 3) species misclassification often occurs between breeds with high similarity. This study demonstrates that machine learning classification models combined with genomic data are effective methods for the classification of local cattle breeds, providing a technical basis for the rapid and accurate classification of cattle breeds in China.

Genomic analysis of inbreeding level, kinship and breed relationships in Creole cattle from South America.

The conservation of animal genetic resources refers to measures taken to prevent the loss of genetic diversity in livestock populations, including the protection of breeds from extinction. Creole cattle populations have suffered a drastic reduction in recent decades owing to absorbent crosses or replacement with commercial breeds of European or Indian origin. Genetic characterization can serve as a source of information for conservation strategies to maintain genetic variation. The objective of this work was to evaluate the levels of inbreeding and kinship through the use of genomic information. A total of 903 DNAs from 13 cattle populations from Argentina, Bolivia and Uruguay were genotyped using an SNP panel of 48 K. Also, a dataset of 76 K SNPs from Peruvian Creole was included. Two inbreeding indices (FROH and Fhat2) and kinship relationships were calculated. In addition, effective population size (Ne), linkage disequilibrium, population composition and phylogenetic relationships were estimated. In Creole cattle, FROH ranged from 0.14 to 0.03, and Fhat2 was close to zero. The inferred Ne trends exhibited a decline toward the present for all populations, whereas Creole cattle presented a lower magnitude of Ne than foreign breeds. Cluster analysis clearly differentiated the taurine and Zebu components (K2) and showed that Bolivian Creole cattle presented Zebu gene introgression. Despite the population reduction, Creole populations did not present extreme values of consanguinity and kinship and maintain high levels of genetic diversity. The information obtained in this work may be useful for planning conservation programmes for these valuable local animal genetic resources.

Evidence of subpopulation diversification and traces of introgression within Canarian camel breed zoometric standard: scope and opportunities for selection

Extant diversity for phenotypic traits is an essential criterion to be considered when ordering priorities for conservation and improvement of animal genetic resources. Concretely, the characterisation of the distinctive body morphometry of a particular group of animals can aid in the design of selective breeding programs, given the strong correlation between body morphology and productive function. The present research aims to characterise an endangered autochthonous camel breed (Canarian camel), mainly relegated to leisure riding, for its body morphology, with a double objective: to explore the phenotypic diversity and structure of the breed for zoometric traits and assess the zoometric profile of this genetic resource that supports its differentiation from other camel breeds. Overall, the results highlight the existence of a high degree of diversity, which may be linked to genetic factors for zoometric traits in an endangered autochthonous breed with traditional in situ breeding schemes, which predicts the success of the implementation of genetic improvement schemes for such functional characteristics. This phenotypic diversity in body morphology could also be a tool for the evaluation of new functional niches within the efforts of functional valorisation of this camel breed for its sustainable conservation. Body morphology traits in the studied camel breed are significantly influenced by sex, physiological status and coat colour.

Demography and Genealogical Analysis of Massese Sheep, a Native Breed of Tuscany.

This study investigates the genealogical and demographic trends of the Massese sheep breed in Tuscany from 2001 to 2021. The Herd Book kept by the Italian Sheep and Goat Breeders Association (Asso.Na.Pa) provided the data. The descriptive statistics were analyzed using JMP software. The pedigree parameters of a total of 311,056 animals (whole population-WP) were analyzed using CFC, ENDOG, and Pedigree viewer software. A total of 24,586 animals born in the period 2007-2021 represented the Reference Population (RP), and 18,554 animals the Base Population (BP). The demographic results showed an inconsistent trend of offspring registration. This study showed a short period of productivity for both ewes and rams, with means of 1.47 and 19.2 registered newborn ewes and rams, respectively. The genealogical analysis revealed incomplete data, highlighting inaccurate assessments of the relationships among the animals, and inbreeding with large differences among provinces. The average inbreeding coefficient in the WP was 1.16%, and it was 2.26% in the RP. The total number of inbreds was 2790 in the WP, with an average FPED of 13.56%, and 2713 in the RP, with an average FPED of 12.82%. The use of pedigree data is a key and economical approach to calculating inbreeding and relationship coefficients. It is the primary step in genetic management, playing a crucial role in the preservation of a breed. The regular updating of genealogical data is the first step to ensuring the conservation of animal genetic resources, and this study is compromised by the lack of such updates.

Gene bank for Animal Genetic Resources in the Republic of Croatia

The Gene bank for domestic animals in the Republic of Croatia is provided by the National Program for the conservation of local and endangered breeds of domestic animals from 2010. For this purpose, the National Gene bank Network was established, which consists of (i) the Gene bank for domestic animals as a central point under the jurisdiction of the Ministry of Agriculture, and (ii) Recognized Gene banks (authorized by the Ministry of Agriculture and operated by organizations, associations and/or authorized institutions). Until 2023, more than 32,000 samples from six types of domestic animals are stored in the Gene bank as follows: cattle (57.7%), horses (13.2%), sheep (12.1%), pigs (10%), donkeys (5%), and goats (1.9%). Tissue samples (29.2%), hair (28%), semen (42.2%) and blood (0.6%) are mainly stored in the Gene bank. The implementation of the National Program is supported by the Reference Expert Network for the Conservation of Animal Genetic Resources, which promotes greater collaboration with stakeholders. Future plans include further characterization of breeds with emphasis on adaptive traits, and the necessary monitoring and implementation of new and improved procedures in the conservation of local breeds.

Conservation of animal genetic resources in the world and its enlightenment to China.

Animal genetic resources in the world are rich and varied. Local species have strong adaptability to the local environment. They are precious resources, and need to be protected by the whole world. In this paper, we summarize the current situation of conservation activities of livestock and poultry resources abroad, including the relevant policies and measures, financial support, genetic material conservation, research projects, and the benefits of conservation animal genetic resources. The actions of conservation of animal genetic resources reflects the increasing recognition of the importance of biodiversity by people around the world. The variety of conservation activities of genetic materials in the world and its benefits reflect that the concept of biodiversity has already been accepted by public and the government. Conservation of animal genetic resources is the primary action for the revitalization of Chinese seed industry. This paper has enlightenment significance for strengthening the conservation of animal genetic resources in China.

A future EU overall strategy for agriculture and forest genetic resources management: Finding consensus through policymakers’ participation

The Genetic Resources Strategy for Europe (GRSE) is an overarching, integrated strategy for the conservation and use of plant, animal and forest genetic resources both the domesticated and wild to ensure more sustainable EU land use policies for the future. It was developed in a three-step consensus seeking iterative process involving EU, national and regional level policymakers, and umbrella organisations representatives. The GRSE provides the missing link between basic biodiversity management (genetic resources) and associated policies and strategies addressing agriculture, forestry and the environment. It also provides guidance for more effective and harmonised genetic resources management in the EU and beyond, and promotes stakeholders’ collaboration in all these areas. In this study, we use a foresight participatory approach to understand the perspectives of identified stakeholders in the adoption of the GRSE at local, national and European level. This methodology enabled researchers to discuss the role of genetic resources management in addressing geographical, economic and societal needs in Europe and beyond, and anticipate the long-term beneficial impacts. The study assessed the outlook for the adoption of the GRSE in the wider policymaking perspective. It applied a three-step Delphi methodology i) interviews, ii) survey, and iii) focus group discussion. It involved a 26-member policy makers panel representing EC and national-level policymakers, and umbrella organisations from across the European Union. Our study reveals a lack of consensus regarding the need to align policy and legal frameworks at national and European levels, but consensus was found in the need to jointly adopt national action plans across member states. Based on these results we identified three main priorities for addressing the gaps within current genetic resources management and conservation approaches: i) align fragmented policy for and develop legislation to harmonise the operational differences between European countries, ii) find funding mechanisms for improving genetic resources infrastructure, research and business development across Europe, and iii) raise awareness and collaboration to involve actors from different levels and sectors.

Animal Husbandry in the Cilento, Vallo di Diano and Alburni National Park: An Economic-Structural Analysis for the Protection and Enhancement of the Territory and Local Resources

This research seeks to deepen the current economic and structural aspects of the livestock farms in the rural territory “Casacastra” (Cilento area), to identify critical issues and development opportunities with the intention to enhance and protect the territory under study also through strengthening forms of local economy. Information about the consistency and the typology of livestock farms present in the study area, and the trend in the last twelve years, were acquired thought the consultation of the national livestock register of the Italian Ministry of Health. Subsequently, a questionnaire was submitted to a sample of farmers to better know the characteristics of the breeders and of the husbandries. The results showed that in the study area, there are 4% of the cattle and sheep herds of the Campania Region, and 7% of those with goats. The most representative farm size is the class with less than 20 animals per breeding type, and between 2010 and 2022, there has been a reduction in the number of all types of analyzed husbandries. The most represented productive orientation is that for meat, while the specialization in the production of milk in sheep and goat breeding is completely absent. The interviews revealed that the breeders are almost all over 40 years of age, with middle and high school qualifications and consolidated experience. The workforce involved in the farms is mainly the family one and often breeders are people engaged in other non-agricultural activities. Despite the permanence in some cases of archaic husbandry methods, the breeders play a key role in the study area for the protection of the territory, and the conservation and enhancement of local animal and plant genetic resources are at risk of extinction. Moreover, the survival of this rural context is linked to the development of new forms of local economy, such as the definition of an experiential rural tourist offer, in which the presence of institutions plays a fundamental role.

Abstracts of the VII American Rabbit Congress. Varadero, Cuba, 12th-13th October, 2022

Last October 12 and 13, 2022, the VII American Rabbit Congress took place within the framework of the 2022 Animal Production and Agro-Development Convention, which was held from October 10 to 14, 2022 at the Plaza América Convention Center in Varadero, Matanzas, Cuba. The VII American Rabbit Congress was developed in a hybrid way (in person and online) with specialists, researchers, professors, students, breeders and producers from different latitudes related to rabbit farming and science. Throughout the conference, a total of 50 people attended in person and 15 online, mainly from Cuba, Mexico, Argentina and Spain. The objective of the meeting was to "Motivate the exchange of experiences and results in rabbit production systems in the Americas, their technical, economic, environmental and social sustainability, and strategies for the improvement, conservation, use and characterization of animal genetic resources" for rabbit farming. The congress opened with a lecture by Juan José Pascual, president of the World Rabbit Science Association (WRSA) on the current state of rabbit farming, followed by three other invited lectures, 18 scientific communications, and a round table on viral hemorrhagic disease, as well as a total of 21 posters. These scientific contributions were directed both to the fundamental areas of knowledge (feeding, genetics, reproduction, pathology), but also to other important aspects of America such as socioeconomics, the value chain, and the use of rabbit farming as a tool to fight against hunger. During the first working day, aspects of the vertical integration of rabbit production, the production and commercialization of rabbits in Cuba, feeding costs and limitations for obtaining raw materials, concern for not total slaughtering rabbits where the virus outbreaks occurred, the political will to eradicate this disease and increase the productivity of the species, the components of the value chains and the need for a correct articulation between all the links in the chain were discussed. On the other hand, during the second working day, there was a debate mainly on aspects related to the role of rabbit farming in society, not only for the production of quality meat, but also for its gender approach, the use of existing local resources in the Latin American countries, and the positive experiences of producers in the promotion of rabbit breeding. The congress closed with good attendance in the room, with a representation of students, producers, specialists, researchers and representatives of state production units and the Cuban Ministry of Agriculture (Director of the National Center for Animal Health and President of the livestock business group).

Analysis for prioritizing risk status and sustainable utilization of cattle, sheep, and goat breeds in Türkiye

Animal Genetic Resources (AnGR) are a warranty for future unknown conditions and needs. Due to this fact, sustainable use and conservation of AnGR have special importance, and to achieve reliable programs for these goals, the risk status of AnGR should be estimated accurately. The aim of this study was to estimate the risk status for three widely used domesticated livestock species of cattle, sheep, and goat in animal production: 6 cattle, 33 sheep, and 15 goat breeds were included in the analysis. Extinction probability (z) was taken as a criterion for the risk status of each breed. Data collection based on survey realized with 215 farmers, field observations and personal interviews were performed for different provinces between 2014 and 2017. Extinction probabilities estimated were ranged from 0.53 to 0.90 with an average of 0.71 for native cattle breeds. Çine Çaparı sheep and Abaza goat had the highest extinction probabilities of 0.97 and 0.87, respectively, while Kıl goat and İvesi sheep breeds had the lowest probabilities of 0.37 and 0.40, respectively. Present study demonstrated that risk analyses of survey data sets may provide useful information concerning the decision-making on conservation efforts for endangered breeds. It should be monitored risk status for breeds to create efficient conservation investments. Considering the limited resources, it is undeniable that while some breeds continued to protect, some others would be removed from conservation programs. To make this task an appropriate manner extinction probability may be used as a criterion for decision-making.

State of conservation of animal genetic resources in Slovakia

To effectively conserve animal genetic resources, countries need to periodically review their conservation efforts and reflect on actual problems and challenges. This study provides a review of animal genetic resources conservation activities, as well as the related existing legislative measures, strategies and funding in Slovakia. We present the development of endangered and supported breeds, discuss the impact of subsidies and the importance of awareness raising, and provide a SWOT analysis of the current animal genetic resources conservation framework in the country. In Slovakia, conservation is primarily based on animal breeding in natural conditions (in situ) without any limitations to breed improvement, and cryoconservation of animal genetic resources is in its initial phase. Most of the funding for conservation measures is provided by the Rural Development Programme. In general, the animal genetic resources system in Slovakia is open in terms of including new breeds eligible for support and this paper shows that the provided subsidies helped to stabilize most of the supported populations. Promoting the presence, characteristics and advantages of local breeds in times of intensive import of exotic breeds into the country is crucial to motivate breeders to prefer local livestock breeds. While the future challenge for the government is to improve conservation and facilitate related activities, research should address not only diversity, pedigree studies and cryoconservation, but also focus on the characterization of animal genetic resources for food security and climate change.

Conservation of animal genetic resources in India-Public aspect

Conservation of animal genetic resources in India-Public aspect

Strategies of promoting sustainable use and conservation of indigenous chicken breeds in Zambia: lessons from low-income countries

This review explores innovative and sustainable strategies for conservation and use of village or indigenous chickens (IC) ( Gallus domesticus) in Zambia with lessons in Sub-Saharan Africa (SSA). Small scale farmers (SSF) have kept IC for hundreds of years to meet their households’ nutritional needs, incomes, social-cultural and religious uses. The commitment exhibited by SSF to keeping indigenous animals has made them the major custodians of essential animal genetic resources in most low-income regions. Between 1991 and 2012, private breeders invested over US$95 million in Zambia’s commercial poultry sector resulting in over 100% increase in the annual production of day-old chicks to 65 million. However, high production costs and low market access hindered the participation of rural farmers hence their continued dependence on IC breeds. The future of IC genetic resources is threatened due to their rapid erosion. In the 2015 biodiversity status report, the Food and Agriculture Organisation, an international body of the United Nations highlighted that over 3.5% of chicken breeds were extinct, nearly 33% were at high risk, and over 67% were of unknown status. Poultry diseases, lack of sustainable conservation strategies and poor use have significantly contributed to these losses. For example, in 2012, 60% of village chickens were reportedly diseased in parts of SSA. If these challenges are not mitigated, the loss of IC genetic resources and the adverse impact on rural communities is inevitable. Further, future research and breeding programs on commercial chickens may be limited due to the erosion of IC genetic resources. This paper reviews lessons and contributes to previous studies that demonstrated how community-based breeding programs and researcher-community-stakeholder engagements potentially enhanced sustainability, adoption of innovative ideas and conservation of local animal genetic resources in selected low-income countries. Further, suggest strategies to promote judicious use and conservation of IC breeds in Zambia.

Production Characteristics and Management Practices of Indigenous Tswana Sheep in Southern Districts of Botswana.

Simple SummaryIndigenous Tswana sheep are an important agricultural resource that largely contribute to the economic, socio-cultural and spiritual aspects of resource-poor farmers. Therefore, as was the aim of this study, exploring and describing the production system in which they are raised, their management including breeding practices is thus crucial to inform researchers, farmers, and policymakers on the formulation of sustainable improvement strategies, sustainable utilization and conservation of these vital animal genetic resources. In the southern part of Botswana, indigenous Tswana sheep are mostly kept by single males aged 51–60 mostly with primary and secondary levels of education. Most Tswana sheep are kept for cash generation through sales, followed by meat consumption and ceremonial obligations, especially in the southern district. Most farmers across districts castrate their young rams at over 3 months of age and most farmers rely on natural pastures for feeding. Feed supplementation is conducted mostly during the dry season while no supplementation is generally practiced during the wet season as there is plenty of palatable forage. Most farmers prefer Tswana rams over exotic breeds because of their ability to survive and reproduce under the Botswana harsh environment. This information is crucial in the formulation of sustainable improvement strategies.The aim of this study was to describe the indigenous Tswana sheep production systems, their management and farmers’ preferred selection traits when selecting breeding rams in four southern districts of Botswana. A total of 105 households; Kgatleng (n = 30), Kweneng (n = 27), southern (n = 24) and south–east (n = 24) districts were interviewed using structured questionnaire. An index-based approach was used to rank farmers’ most preferred traits for their production systems. Data were analyzed using Statistical Package for Social Sciences. The Chi-square test was used to assess the statistical significance among categorical variables. The results indicated that indigenous Tswana sheep are mainly kept by males, single people, aged between 51 and 60 years possessing primary and secondary education. Management practices across the districts include castration, health care and supplementation mostly during the dry season. Superior fitness traits of indigenous Tswana rams over exotic rams were considered more important when selecting breeding rams in Kgatleng, Kweneng and south–east while in the southern district, rams were mainly selected based on body size. Most farmers kept breeding rams while those who did not keep rams depended on communal rams for service. This information is important in designing successful breeding programs and strategies for the conservation and sustainable utilization of indigenous Tswana sheep genetic resources.

Characterization of duck germplasm of eastern hill and plateau region of Jharkhand

Information about phenotypic characteristics is a basic need in animal genetic resource conservation and improvement. Phenotypic characteristics of Jharkhand ducks from six districts (Palamu, Gadhwa, Latehar, Lohardaga, Khuti, and Simdega) were studied. Data on morphological and morphometric traits were analyzed. The results revealed that the predominant plumage colours of the head, neck, breast, wings and tails were black (56.25%) in drake, and black and white mix (65.33%) in duck; white and black/brown mix (62.50%) in drake, and white (79.33%) in duck; brown (41.25%) in drake, and white and black/brown mix (52.67%) in duck; black/brown and white mix (43.75%) in drake and duck (74.67%); black in drake (75%) and duck (90%) respectively. The dominant bill colour in drake was greenish black (56.25%) followed by orange (25%) and duck bill colour was black (58.67%) followed by orange (22%), whereas dominant eye colour was brown in both the sexes. The predominant shank and web colour were orange (65.0%) in drake and duck (68.0%) respectively. The Jharkhand ducks are unique in their morphological features with attractive black and white plumage colour pattern. The ducks are well acclimatised to local agro-climatic conditions with less input from duck farmers. The hatching and brooding process are natural. The average egg production was 66.92±2.00 eggs. The average adult body weights of drake and ducks were 1.64±23.19 and 1.51±30.09 kg, respectively. Variations were observed in qualitative traits. The observed phenotypic diversity in Jharkhand local ducks could be useful in designing breeding programs and selection.

Payments for Conservation of Animal Genetic Resources in Agriculture: One Size Fits All?

Simple SummaryMaintaining minimum population sizes for local livestock breeds is a key goal in the conservation of animal genetic resources. As markets and livestock production systems have tended to favour a narrow base of high-output improved breeds, countries have had to use financial and other incentives to motivate breeders to keep local breeds. This paper explores the potential for more cost-effective alternatives to the most commonly used financial incentive, a fixed payment per animal or livestock unit. We compare the current fixed payment incentives for local breeds under the Slovenian Rural Development Programmme with those instead determined through a competitive tender approach. A stated preference survey was realised to determine the conditions under which breeders would be willing to participate in such an incentive system based on differentiated payments. Willingness to accept (WTA) payment for conservation was found to differ significantly from actual payment levels, being lower for the local sheep and goat breeds, and higher for the local pig breed. This suggests that implementation of differentiated payments would be more cost-effective; particularly when accompanied by measures to streamline administrative requirements, improve access to breeding stock and target support for local breed market valorisation (e.g., promotion of value chains based on designated quality schemes).Local livestock breeds in Slovenia have been eligible for financial incentives in the form of a fixed payment per livestock unit (LU) since 2002. The scheme has however not been successful in reversing the erosion of animal genetic resources (AnGR). This paper investigates an alternative, whereby incentive payments would better reflect breeders’ actual opportunity costs. The paper contributes to the limited existing body of knowledge related to the use of tender mechanisms in the design of the payments for agrobiodiversity conservation schemes (PACS), particularly for AnGR. Empirical findings draw on the results of a stated preference survey involving 301 farmers in Slovenia, engaging, or being potentially able to engage, in the rearing of local pig, sheep and goat breeds. Interval and logistic regression model results suggest that willingness to accept (WTA) conservation support significantly differs from actual payment levels. The estimated WTA was found to be 27% lower for the local sheep and goat breeds and 5% higher for the local pig breed, suggesting that differentiated incentive payments would provide a more cost-effective alternative. Additional analysis of breeders’ preferences and motives for engaging in local livestock breed production further informs understanding regarding AnGR conservation policy and the importance of accompanying actions to reverse negative population trends. These include reducing administrative barriers and enhancing the market valorisation of local breeds.

AUTOCHTHONOUS BREEDS OF DOMESTIC ANIMALS AND CONSERVATION MEASURES

Recently, highly productive breeds of various species of domestic animals have been used in livestock production, which has resulted in the destruction of indigenous breeds of domestic animals around the world, even in our area. This is the first reason why indigenous races and strains have been endangered. Another reason is that domestic, indigenous breeds were crossed with specialized breeds, which were imported, and in that way their genetic diversity was negatively affected. Resistance is lost, adaptation to the conditions in which they were created, the ability to survive in nature. Indigenous breeds of different species of domestic animals, which are recognized in the Republic of Srpska (BiH) are gatačko cattle and buša (cattle), Vlašić pramenka, Podveleška pramenka, Kupres pramenka (sheep), domestic Balkan horned goat (goats), Bosnian mountain horse (horses), mangulica (pigs) and pogrmuša hen or živičarka hen (poultry). By acceding to international conventions, BiH /Republic of Srpska has committed itself to establishing a system of measures that will enable the conservation of biological diversity and the protection of indigenous and endangered breeds of domestic animals. The choice of a strategy for the conservation of diversity, the establishment of an adequate conservation scheme, and the implementation of a conservation strategy are some of the key elements of any process for the conservation of genetic diversity. Preservation of autochthonous and protected breeds of domestic animals is possible through preservation in the original environment (in situ) and preservation outside the original environment (ex situ). There is a possibility of combining these models of conservation of animal genetic resources.

Describing variability in pig genes involved in coronavirus infections for a One Health perspective in conservation of animal genetic resources

Coronaviruses silently circulate in human and animal populations, causing mild to severe diseases. Therefore, livestock are important components of a “One Health” perspective aimed to control these viral infections. However, at present there is no example that considers pig genetic resources in this context. In this study, we investigated the variability of four genes (ACE2, ANPEP and DPP4 encoding for host receptors of the viral spike proteins and TMPRSS2 encoding for a host proteinase) in 23 European (19 autochthonous and three commercial breeds and one wild boar population) and two Asian Sus scrofa populations. A total of 2229 variants were identified in the four candidate genes: 26% of them were not previously described; 29 variants affected the protein sequence and might potentially interact with the infection mechanisms. The results coming from this work are a first step towards a “One Health” perspective that should consider conservation programs of pig genetic resources with twofold objectives: (i) genetic resources could be reservoirs of host gene variability useful to design selection programs to increase resistance to coronaviruses; (ii) the described variability in genes involved in coronavirus infections across many different pig populations might be part of a risk assessment including pig genetic resources.