This paper investigates the predefined-time tracking consensus issue for a class of non strict-feedback nonlinear multi-agent systems (MASs) under input delay and error performance constraints by adaptive backstepping control strategy. Firstly, the impact of input delay is eliminated by Pade approximation. With the help of an intermediate state variable, the considered MASs with input delay are revised as the independent-delay systems. Secondly, under constrained distributed error and corrected nth virtual error, the ln-type barrier Lyapunov function (BLF) is adopted to solve the error performance constrains and reduce the complexity of differentiation. Meanwhile, an adaptive predefined-time controller is designed to realize the tracking purpose by backstepping method and radial basis function neural networks (RBFNNs) approximation. Finally, a numerical simulation example and a practical application are presented to test the effectiveness for the proposed control strategy.