Consciousness Energy Healing Treatment Research Articles

LC-MS and GC-MS-Based Assessment of the Impact of Consciousness Energy Healing Treatment on the Isotopic Abundance Ratios of Sulfamethoxazole

Modern Approaches in Drug Designing LC-MS and GC-MS-Based Assessment of the Impact of Consciousness Energy Healing Treatment on the Isotopic Abundance Ratios of Sulfamethoxazole Mahendra Kumar Trivedi1, Alice Branton1, Dahryn Trivedi1 and Snehasis Jana2* 1Trivedi Global, Inc., Henderson, USA 2Trivedi Science Research Laboratory Pvt. Ltd., India *Corresponding author: Jana S, Trivedi Science Research Laboratory Pvt. Ltd., Thane (W), Maharashtra, India Submission: May 26, 2021;Published: June 18, 2021 DOI: 10.31031/MADD.2021.03.000563 ISSN: 2576-9170 Volume3 Issue3

Structural Characterization and Isotopic Abundance Ratio Analysis of the Consciousness Energy Healing Treated Ofloxacin

Ofloxacin is an antibiotic, useful against the number of bacterial infections. This scientific investigation was performed to identify the impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on the structural properties and the isotopic abundance ratio of ofloxacin using sophisticated analytical techniques. Ofloxacin sample was divided into control and treated parts. Only the treated ofloxacin received the Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The LC-MS spectra of both the samples of ofloxacin at retention time 3 minutes exhibited the mass of the protonated molecular ion peak at m/z 362.17 (M+H)+. The chromatographic peak area% of the treated ofloxacin (52.4%) was increased by 2.03% compared to the control sample (51.36%). The LC-MS based isotopic abundance ratio of PM+1/PM in the Biofield Treated ofloxacin was significantly increased by 22.43% compared with the control sample. Similarly, the GC-MS based isotopic abundance ratio of PM+1/PM in the Biofield Treated ofloxacin was significantly increased by 19.24% compared with the control sample. The LC-MS and GC-MS based isotopic abundance ratio of PM+1/PM (2H/1H or 15N/14N or 13C/12C or 17O/16O) was significantly increased in the Biofield Treated ofloxacin as compared to the control sample. Thus,2H, 15N, 13C, and17O contributions from (C18H21FN3O4)+ to m/z 363.17 in the treated ofloxacin were significantly increased compared with the control sample. The increased isotopic abundance ratio of the Trivedi Effect®-Consciousness Energy Healing Treated ofloxacin may increase the intra-atomic bond strength and increase its physical stability. The new form of treated ofloxacin would be more stable, better soluble, and bioavailable compared to the control sample. It would be more useful to design efficacious pharmaceutical formulations that might offer better therapeutic response against infections in the urethra, urinary tract, gonorrhoea, pneumonia, infectious diarrhoea, bronchitis, cellulitis, bacterial infection of the eye and ear, multidrug-resistant tuberculosis, prostatitis, otitis media, plague, etc.

Effect of Biofield Treatment on Blood, Hepatic, and Cardiac Biomarkers in Vitamin D3 Deficiency Diet (VDD) Induced Sprague Dawley Rats

A novel proprietary formulation was designed that consist of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The study objective was to evaluate the impact of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD) for immunomodulatory activity. The test formulation was divided into two parts. One part was denoted as the untreated test formulation without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of total leukocytes count (TLC) were significantly increased by 23.1% and 17.44% in the Biofield Energy Treated test formulation (G5) and Biofield Energy Treated test formulation from day -15 (G7) groups, respectively compared with the disease control group (G2) induced by VDD. Moreover, the level of neutrophils was significantly increased by 71.68%, 92.92% (p≤0.01), 42.48%, and 49.56% in the G5, G7, Biofield Energy Treatment per se plus Biofield Energy Treated test formulation from day -15 (G8), and Biofield Energy Treatment per se animals plus untreated test formulation (G9) groups, respectively compared with the G2 group. Monocyte level was also increased by 23.53%, 17.65%, and 29.41% in the G5, Biofield Energy Treatment per se to animals from day -15 (G6), and G9 groups, respectively compared to the G2 group. Hepatic biomarker like alkaline phosphatase (ALP) was significantly reduced by 14.98% and 23.07% (p≤0.05) in the G7 and G9 groups, respectively as compared to the G2 group. Besides, cardiac biomarker like creatine kinase myocardium band (CK-MB) was also significantly (p≤0.05) reduced by 20.99% in the G6 group compared with the G2. Altogether, results suggested that the Biofield Treated test formulation and Biofield Energy Treatment per se significantly increased immune-related parameters, which might be beneficial for the management of immune-compromised patients as well as to boost-up the immunity in healthy peoples. The results showed a significant slowdown of disease progression and all other disease-related complications/symptoms in the preventive Biofield Energy Treatment group per se and the Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control group.

Study of the Consciousness Energy Healing Treatment and It’s Effect on the Isotopic Abundance Ratio of Ascorbic Acid (Vitamin C)

Ascorbic acid is a water-soluble vitamin (Vitamin C) essential for both the plants and animals for the metabolic process. In this study, the liquid chromatography-mass spectrometry (LC-MS) analytical technique was used to characterize the structural properties and isotopic abundance ratio to evaluate the effect of the Trivedi Effect®-Consciousness Energy Healing Treatment on L-ascorbic acid compared to the control sample. The ascorbic acid sample was divided into control and treated parts. Only the treated part received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy Healer, Mahendra Kumar Trivedi. The control and treated samples showed a chromatographic peak at retention time (Rt) 1.8 minutes exhibited the deprotonated molecular ion peak at m/z 175 (M-H)- (calculated for C6H7O6-, 175.02) in the mass spectra. The peak area of the treated sample (12817614.01) was significantly increased by 8.81% compared to the control sample (11779918.9). The LC-MS based isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in the treated ascorbic acid was significantly increased by 23.22% compared with the control sample. Thus,13C, 2H, and17O contributions from (C6H7O6)- to m/z 176 in the treated ascorbic acid were significantly increased compared with the control sample. The increased isotopic composition of the treated ascorbic acid might have altered the neutron to proton ratio in the nucleus. The changes in isotopic abundance could be due to changes in nuclei possibly through the interference of neutrino particles via the Trivedi Effect®-Consciousness Energy Healing Treatment. The increased isotopic abundance ratio and peak area of the treated ascorbic acid may increase the intra-atomic bond strength and its stability. This novel ascorbic acid after the Trivedi Effect®-Consciousness Energy Healing Treatment would be very useful to design more efficacious pharmaceutical formulations against scurvy, obesity, cardiovascular diseases, hypertension, rheumatoid arthritis, Alzheimer's disease, cancer, etc.

Characterization of the Consciousness Energy Healing Treated Cholecalciferol Using LC-MS and GC-MS Spectrometry

Vitamin D3 (cholecalciferol) is a fat-soluble vitamin, which widely used for the prevention and treatment rickets, osteoporosis, arthritis, Parkinson’s and Alzheimer’s diseases, autoimmune disease, dementia, glucose intolerance, etc. The impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on the structural properties and the isotopic abundance ratio of cholecalciferol were evaluated using LC-MS and GC-MS spectroscopy. The test sample cholecalciferol was divided into control and treated parts. Only, the treated cholecalciferol was received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Dahryn Trivedi. The LC-MS spectra of both the samples at retention time (Rt) ~22 minutes exhibited the mass of the molecular ion peak at m/z 385.25 (calcd for C27H45O+, 385.35). The LC-MS based isotopic abundance ratio of PM+1/PM in the treated cholecalciferol was increased by 0.74% compared with the control sample. But, the GC-MS based isotopic abundance ratio of PM+1/PM and PM+2/PM in the treated cholecalciferol was significantly increased by 66.39% and 62.69%, respectively compared with the control sample. Hence,13C, 2H, 17O, and 18O contributions from C27H44O+ to m/z 386 and 387 in the treated cholecalciferol were significantly increased compared with the control sample. The isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 17O/16O) and PM+2/PM (18O/16O) in the treated cholecalciferol were significantly increased as compared to the control sample. The increased isotopic composition of the Trivedi Effect®-Consciousness Energy Healing Treated cholecalciferol might have altered the neutron to proton ratio in the nucleus via the possible mediation of neutrino. The increased isotopic abundance ratio of the treated cholecalciferol may increase the intra-atomic bond strength, increase its stability. The new form of cholecalciferol would be better designing novel pharmaceutical formulations that might be more stable and more efficacious for the prevention and treatment of various diseases such as vitamin D deficiency, rickets, osteoporosis, arthritis, multiple sclerosis, cancer, diabetes mellitus, mental disorders, cardiovascular diseases, hypertension, infections, influenza, cognitive impairment in older adults, Parkinson’s and Alzheimer’s diseases, autoimmune disease, dementia, glucose intolerance, multiple sclerosis, etc.

Evaluation of the Isotopic Abundance Ratio of Consciousness Energy Healing Treated L-Tryptophan Using LC-MS Spectrometry

L-tryptophan is an essential α-amino acid, necessary for the normal growth in newborns, nitrogen balance in adults, protein synthesis, precursor of serotonin, melatonin, niacin, and albeit inefficiently in human, also the precursor of indole alkaloids and auxins in plants. This current study was designed to investigate the impact of the Trivedi Effect®-Biofield Energy Healing Treatment (Blessing) on the structural properties and the isotopic abundance ratio of L-tryptophan using LC-MS analytical technique. L-tryptophan sample was divided into two parts, one part of L-tryptophan was considered as the control sample (no Biofield Energy Treatment was provided), while the second part was treated with the Trivedi Effect®-Consciousness Energy Healing Treatment/Blessing remotely by a renowned Biofield Energy Healer, Dahryn Trivedi and termed as the treated sample. The mass spectra of both the control and treated samples with respect to the chromatographic peak at retention time (Rt) 2.1 minutes exhibited the mass of the molecular ion peak adduct with hydrogen ion at m/z 205.08 (calcd for C11H13N2O2+, 205.1), along with low molecular fragmented mass peaks at m/z 188, 159, and 102 for C11H12N2O2+, C10H11N2+, and C8H6+, respectively were also observed. The isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 15N/14Nor17O/16O) in the treated L-tryptophan was significantly increased by 35.93% compared with the control sample. Hence,the 13C, 2H, 15N, and 17O contributions from C11H13N2O2+ to m/z 206.08 in the treated L-tryptophan was significantly increased compared to the control sample. It could be hypothesized that the changes in the isotopic abundance and mass peak intensities due to the modification in nuclei possibly through the interference of neutrino particles using the Trivedi Effect®-Consciousness Energy Healing Treatment. The Biofield Energy Treated/Blessed L-tryptophan with increased stable isotopic abundance ratio might have changed the physicochemical properties with higher force constant in the molecule. The new form of treated L-tryptophan would be a better and more stable in the supplements, nutraceutical, and pharmaceutical formulations, which would be advantageous for the prevention and treatment of pellagra, depression, kynurenine. It could also maintain the normal label of tryptophan and avoid increase of its metabolite, lower the neurotoxin and a metabotoxin behavior, glutaric aciduria type I (glutaric acidemia type I) disorder, eosinophilia-myalgia syndrome (EMS), incurable and sometimes fatal flu-like neurological condition, etc. As tryptophan is the precursor for the plant hormones like indole alkaloids and auxins, hence, this treated L-tryptophan would be advantageous for the improvement of yield, productivity, and quality of crops and other plants.

Evaluation of Biofield Energy Healing Based Test Formulation on Thyroid Biomarkers in Vitamin D3 Deficiency Diet (VDD) Induced Animal Model

This present study evaluated the impact of Consciousness Energy Healing Treatment (the Trivedi Effect) on a novel test formulation in maleSprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD) for the estimation of thyroid biomarkers such as calcitonin, parathyroid (PTH),thyroid stimulating hormone (TSH), and thyroxine (T4). A novel proprietary test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The novel test formulation was divided into two parts; one part was defined as untreated test formulation, while the other of test formulation and the three group of animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of calcitonin (pg/mL) was significantly increased by 22.7%, 4.2%, 44.9%, 72%, and 67.7% (p≤0.01) in the Biofield Energy Treated test formulation group (G5), Biofield Energy Treatment per se (G6), 15 days pre-treatment of Biofield Energy Treated test formulation (G7), 15 days pre-treatment of Biofield Energy Treated test formulation to the Biofield Energy Treated animals (G8), and Untreated test formulation to the Biofield Energy Treated animals (G9) groups, respectively as compared with the untreated group (G4). However, the level of TSH was reported to be decreased by 23.3%, 20.4%, and 37.3% in the G7, G8, and G9 respectively as compared with the G4. The level of PTH was decreased by 30.6% and 10.9% in the G6 and G9, respectively, while it was increased by 9.7% in the G5 as compared with the G4. Similarly, the level of T4 was increased by 40.5%, 20.7%, and 20.8% in the G5, G7, and G8 groups respectively as compared with the G4. In conclusion, the Biofield Treated test formulation and Biofield Energy Treatment per se significantly improved the thyroid biomarkers in serum that can be used against many thyroid disorders such hyperthyroidism, goitre, thyroid nodules, thyroid cancer, thyroid hormone resistance, Hashimoto’s thyroiditis, and many more. Overall, the results showed the significant slowdown the disease progression and disease-related all other complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) compared with the disease control group. Keywords: Biofield Treatment; Thyroid Biomarkers; The Trivedi Effect; Thyroxine; Vitamin D3 deficiency diet; PTH; Thyroid Disorders

Effect of Consciousness Energy Healing Treatment on the Metal Profile and Properties of Tellurium

Effect of Consciousness Energy Healing Treatment on the Metal Profile and Properties of Tellurium

Investigation on the Impact of Biofield Energy Treatment on the Physical, Thermal and Spectroscopic Characteristics of Zinc Chloride

Zinc is an essential trace mineral that plays an important role in the human body as a regulator for different enzymatic systems. It is used in various supplements in the form of zinc chloride to meet the daily requirements. This study was designed with the aim to investigate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment on the various physical, thermal, and spectral properties of zinc chloride. The study involves dividing the zinc chloride sample in two parts, followed by keeping the first part untreated and named it the control sample. Besides, The Trivedi Effect®- Biofield Energy Healing Treatment was provided to the other part remotely by the renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and the sample was termed as Biofield Energy Treated sample. Later on, both the samples were analyzed using PXRD, PSA, DSC, UV-Vis, and FT-IR analytical techniques. The PXRD analysis revealed the significant changes in the crystallite sizes (from -66.68% to 25.02%) and the relative peak intensities (from -81.35% to 141.22%) along with 26.46% decrease in the average crystallite size of the Biofield Energy Treated sample, compared with the control sample. Additionally, the treated sample showed reduction in the particle sizes d10, d50, d90, and D(4,3) values by 92.18%, 23.24%, 14.48%, and 29.31%, respectively as compared to the control sample. However, the surface area of the treated sample was observed to be significantly increased by 224.57%, compared to the control sample. The DSC analysis revealed slight increase in the melting and decomposition temperature of the Biofield Energy Treated sample by 0.84% and 3.62%, respectively; along with the significant increase in the latent heat of melting and decomposition by 24.85% and 123.23%, respectively, compared to the control sample. It showed that the thermal stability of the treated sample was significantly increased. However, the UV-Vis and FT-IR studies revealed that the structural properties of the control and treated samples were remained same. The overall study represents that The Trivedi Effect®- Energy of Consciousness Healing Treatment may be used to generate a polymorphic form of zinc chloride, which might possess enhanced solubility, dissolution and bioavailability profile along with improved thermal stability, compared to the control sample. Such properties of the treated zinc chloride may ensure its efficacy, stability and safety during the process of shipment, handling, and storage; thus, The Trivedi Effect®-Energy of Consciousness Healing Treated zinc chloride would be useful for designing the better formulation with enhanced bioavailability, stability and efficacy profile.

Evaluation of Physicochemical, Spectral and Thermal Properties of Energy of Consciousness Healing Treated Iron Sulphate

Iron sulphate is used in the treatment of iron deficiency anaemia and other chronic disorders such as heart and kidney diseases. This study has the objective to analyze the impact of The Trivedi Effect®-Energy of Consciousness Healing Treatment on the physicochemical, spectroscopic and thermal properties of iron sulphate using various analytical techniques. In this, the test compound, i.e., iron sulphate was divided into two parts; one as control (without Biofield Energy Treatment), and the other as Biofield Energy Treated, which received the Biofield Energy Treatment remotely by the renowned Healer, Mr. Mahendra Kumar Trivedi. The PXRD analysis of the Biofield Energy Treated sample showed significant alterations in the range of -39.49 to 301.40% in the relative intensities, and from -15.40 to 33.36% in the crystallite sizes, compared with the control sample. The average crystallite size of the treated sample was also increased by 4.98% as compared to the control sample. The particle sizes in the treated sample at d10, d50, d90 and D(4,3) values were significantly increased by 67.12%, 47.72%, 33.18% and 42.01%, respectively; whereas, the specific surface area was significantly reduced by 38.39%, compared with the control sample. The TGA thermograms showed three steps of thermal degradation in which, the weight loss of Biofield Energy Treated sample in the first and second step was reduced by 5.82% and 16.09%, respectively, while, it was increased by 6.78% in the third step, compared to the control sample. The total weight loss in the treated sample was also reduced by 2.76%, along with slight alteration in the maximum thermal decomposition temperature, compared with the control sample. The DSC analysis showed the decrease in the melting temperatures of the 1st, 2nd and 4th peaks by 8.24%, 19.29%, and 0.61%, respectively, while 4.57% increase in the 3rd peak of the treated sample, compared with the control sample. The latent heat of fusion (ΔH) corresponding to the 1st, 2nd, 3rd and 4th peaks of the treated sample also showed alterations by -92.29, -86.29, 60.92, and 6.37%, respectively, compared with the control sample. The Trivedi Effect®-Consciousness Energy Healing Treatment might produce a novel polymorphic form of iron sulphate having increased crystallite and particle size along with enhanced thermal stability. It may help in improving the quality, safety and stability during the process of handling, storage, and shipment of the iron sulphate with better therapeutic response against iron deficiency anaemia.

"Impact of Consciousness Energy Healing Treatment on the Isotopic Abundance Ratio of Folic Acid"

Annals of Reviews & Research open access journal that publishes high-quality solicited and unsolicited articles, from various types of subject areas ranging from management to engineering sciences, accounting, criminal sciences, etc. which deal with innovative research work.

Evaluation of the Impact of Consciousness Energy Healing Treatment on the Isotopic Abundance Ratio of L-Cysteine Using LC-MS Spectrometry

Annals of Chemical Science Research Evaluation of the Impact of Consciousness Energy Healing Treatment on the Isotopic Abundance Ratio of L-Cysteine Using LC-MS Spectrometry Branton A1, Trivedi MK1, Trivedi D1 and Jana S2* 1Trivedi Global, Inc.,USA 2Trivedi Science Research Laboratory Pvt. Ltd., India *Corresponding author: Jana S, Trivedi Science Research Laboratory Pvt. Ltd., India Submission: December 07, 2020Published: January 22, 2021 DOI: 10.31031/ACSR.2021.02.000537 Volume2 Issue3January, 2021

Evaluation of the Impact of Consciousness Energy Healing Treatment on the Isotopic Abundance Ratio of Folic Acid Using LC-MS Spectrometry

The present review focused on synthesis of nanoparticles using an eco-friendly approach through microorganisms, especially fungi. Various physical and chemical methods and techniques have been developed by scientists and researchers to manufacture nanoparticles. But nanoparticles synthesized in these forms are not eco-friendly and often allow toxic compounds to be released which could have adverse effects on nature and in human beings. It is therefore important to develop eco-friendly and economical techniques for nanoparticle synthesis and to respond to green approaches by using natural microorganisms such as bacteria, fungi and algae, etc. which are capable of producing nanoparticles. Today’s biologist works on most of the biological material for nanoparticle synthesis but shaping all of these is focused on fungi for nanoparticles that can help to reduce time and we can obtain nanoparticles in desired size and shape. This is also the key reason for using fungal microbes for nanoparticle synthesis to grow perfect and non-toxic material production, which can help to reduce environmental impacts, increase energy efficiency and mitigate environmental pollution. The present review therefore emphasized the biological method for the synthesis of nanoparticles by fungi, the development of different types of metal nanoparticles by fungal microbe and the application of novel nanoparticles in the current emerging sector.

Influence of Consciousness Energy Healing Treatment on the Isotopic Abundance Ratio of Sulfamethoxazole Using LC-MS and GC-MS Spectrometry

Sulfamethoxazole is a broad spectrum antibiotic especially used for the treatment of infections caused by bacteria. This study was performed to investigate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment on the structural properties and the isotopic abundance ratio of sulfamethoxazole using LC-MS and GC-MS spectroscopy. Sulfamethoxazole sample was divided into two parts, one part of sulfamethoxazole was considered as control (no Biofield Energy Treatment was provided), while the second part was treated with The Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Alice Branton and termed as a treated sample. The LC-MS spectra of both the samples at retention time (Rt) 2.5 minutes exhibited the mass of the deprotonated molecular ion peak at m/z 252 [M-H]- (calculated for C10H10N3O3S-, 252.04). The LC-MS based isotopic abundance ratio of PM+1/PM in the treated sulfamethoxazole was significantly increased by 44.67% compared with the control sample. Thus, 13C, 2H, 15N, 17O, and 33S contributions from (C10H10N3O3S)- to m/z 253 in the treated sample were significantly increased compared with the control sample. The control and treated sulfamethoxazole showed the presence of a chromatographic peak at the retention time of 16.91 min in the GC-MS chromatograms. The peak area% of the treated sample was significantly increased by 80.3% compared to the control sample. The GC-MS based isotopic abundance ratio of PM+1/PM and PM+2/PM in the treated sulfamethoxazole was significantly increased by 24.13% and 90.53%, respectively compared with the control sample. Hence, 13C, 2H, 15N, 17O, 18O, 33S, and 34S contributions from (C10H10N3O3S)+ to m/z 254 and 255 in the Biofield Energy Treated sample were significantly increased compared with the control sample. The isotopic abundance ratios of PM+2/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O or 33S/32S) and PM+2/PM (18O/16O or 34S/32S) in the treated sulfamethoxazole were significantly altered compared to the control sample. It can be assumed that the changes in isotopic abundance and mass peak intensities could be due to changes in nuclei possibly through the interference of neutrino particles via The Trivedi Effect®- Consciousness Energy Healing Treatment. The new form of sulfamethoxazole would be better designing novel pharmaceutical formulations that might offer better solubility, dissolution, absorption, bioavailability and therapeutic response against urinary tract infections, ear infections, tuberculosis, traveler’s diarrhea, shigellosis, bronchitis, and pneumocystis jiroveci pneumonia, etc.

Physicochemical, Thermal and Spectroscopic Characterization of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract

Ashwagandha root extract is widely used in nutraceutical preparations due to its broad range of pharmacological activities. The objective of this study was to analyse the impact of Biofield Energy Treatment (The Trivedi Effect®) on the physicochemical, thermal and spectral properties of ashwagandha root extract by using various analytical techniques. The sample of ashwagandha root extract was divided into two parts in which one part was kept untreated and called as control sample; while the other part was provided with the Consciousness Energy Healing Treatment remotely by the Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and termed as Biofield Energy Treated sample. The study reported that the particle size values at d10, d50, d90, and D (4,3) in the Biofield Energy Treated sample was significantly decreased by 9.23%, 9.04%, 11.86%, and 10.59%, respectively compared with the control sample. Thus, the surface area of the treated sample was significantly increased by 10.75% compared to the control sample. The PXRD analysis showed that the control as well as the Biofield Energy Treated samples was amorphous in nature. The DSC analysis showed that the onset evaporation temperature and latent heat of vaporization were significantly altered by 2.18% and-10.29%, respectively in the Biofield Energy Treated sample compared with the control sample. The TGA analysis indicated the four step thermal degradation of both the samples. The 1st, 2nd, 3rd, and 4th steps of thermal degradation of the treated sample showed an alteration in the weight loss by -6.59%, 1.97%, -1.80%, and -8.65%, respectively along with 0.81% reduction in the total weight loss, compared with the control sample. Besides, the maximum thermal degradation temperature (Tmax) in the control sample was observed at 234.17°C and 365.12°C for the two broad peaks, which was decreased by 1.73% and 0.82%, respectively compared to the control sample. The overall analysis suggests that the Energy of Consciousness Healing Treatment might enhance the solubility, absorption, and bioavailability profile of ashwagandha root extract along with altered thermal stability. Such altered properties might help in designing better pharmaceutical and nutraceutical preparations; thereby provide better therapeutic response against various diseases such as amnesia, arthritis, anxiety, cancer, impotence, neurodegenerative, and cardiovascular diseases.

Investigation of Impact of Biofield Energy Healing Treatment on Physicochemical, Thermal and Spectral Properties of Magnesium Gluconate

Magnesium plays important role in the functioning of cells, nerves, muscles, and bones of human body. It is incorporated in the form of magnesium gluconate in various pharmaceutical and nutraceutical supplements due to its wide applications. This study was done with the objective to analyse the effect of The Trivedi Effect®- Energy of Consciousness Healing Treatment on the physical, thermal, and spectral properties of magnesium gluconate. In this regard, the sample of magnesium gluconate was divided in two parts, in which first part was kept untreated and termed as control sample. Besides, the second part was remotely treated by the renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi with The Trivedi Effect®- Biofield Energy Healing Treatment and termed as Biofield Energy Treated sample. Consequently, both the samples were analyzed using PXRD, PSA, DSC, TGA/DTG, UV-Vis, and FT-IR analytical techniques. The PXRD analysis revealed that the crystallite sizes and relative peak intensities of the treated sample showed variations in the range of-25.01% to 185.79% and 6.99% to 32.99%, respectively along with 13.03% increase in average crystallite size as compared to the control sample. The particle sizes d10, d50, d90, and D(4,3) values of the treated sample were significantly decreased by 3.70%, 21.88%, 22.03%, and 21.16%, respectively, along with 10.75% increase in the surface area as compared to the control sample. The DSC studies showed minor decrease in melting point (0.10%) with 3.74% increase in the latent heat of fusion of the treated sample, compared to the control sample. Besides, The TGA/DTG analysis represented alterations in the weight loss pattern and maximum thermal degradation temperature (Tmax) of the treated sample. The analysis revealed three steps thermal degradation, in which the variation was observed from -6.79% to 10.14% in terms of weight loss along with 0.24% increase in the total weight loss of the treated sample, compared to the control sample. The Tmax of the first two peaks were found to be increased by 6.90% and 1.28%, while 3rd and 4th peaks were slightly decreased by 0.57% and 0.24% in the treated sample, compared to the control sample. The Energy of Consciousness Healing Treatment might produce a new polymorphic form of magnesium gluconate, which may have a better solubility, absorption and bioavailability profile along with altered thermal stability compared to the untreated sample. Thus, the Biofield Energy Treated magnesium gluconate would be helpful in designing the better nutraceutical/pharmaceutical formulations with better therapeutic responses against the magnesium deficiency.

The LC-MS and GC-MS Based Isotopic Abundance Ratio Analysis of the Consciousness Energy Healing Treated Silver Sulfadiazine

Silver sulfadiazine is a topical antibiotic helps to prevent and treat wound infections in patients with severe burns. This research was performed to evaluate the impact of the Trivedi Effect®- Consciousness Energy Healing Treatment on the structural properties and the isotopic abundance ratio of silver sulfadiazine using spectroscopic and chromatographic techniques. The test sample silver sulfadiazine sample was divided into control and treated parts. Only the treated part of silver sulfadiazine was received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Dahryn Trivedi. The LC-MS spectra of both the samples at retention time (Rt ) 4.1 minutes exhibited the mass of the protonated molecular ion peak at m/z 251.08 (calculated for C10H11N4 O2 S+ , 251.06) was found to be sulfadiazine. The chromatographic peak area of the treated silver sulfadiazine was significantly increased by 20.35% compared to the control sample. The result indicated that the solubility profile of the treated silver sulfadiazine must have increased significantly after the Biofield Energy Treatment compared with the control sample. The LC-MS based isotopic abundance ratio of PM+1/PM in the treated sulfadiazine was significantly increased by 31.88% compared with the control sample. Similarly, the GC-MS based isotopic abundance ratio of PM+1/PM and PM+2/PM in the treated sulfadiazine was significantly increased by 9.74% and 15.13%, respectively compared with the control sample. Hence, 13C, 2 H, 15N, 17O, 33S, and 34S contributions from (C7 H9 N2 O2 S)+ to m/z 186 and 187 in the treated sulfadiazine were significantly increased compared to the control sample. Thus, the isotopic abundance ratios of PM+1/PM (2 H/1 H or 13C/12C or 15N/14N or 17O/16O or 33S/32S) and PM+2/PM (34S/32S) in the treated sulfadiazine were significantly increased compared to the control sample. The changes in isotopic abundance and peak area might be due to changes in nuclei possibly via the interference of neutrino particles controlled by the Trivedi Effect®-Consciousness Energy Healing Treatment. The increased isotopic abundance ratio of the treated silver sulfadiazine might improve the chemical bond strength, led to increase the stability in the body. This novel silver sulfadiazine would be more efficacious in pharmaceutical formulations which might offer better solubility, bioavailability, and therapeutic response against bacterial infections, superficial and partial thickness burn injuries, etc.

Chromatographic and Spectroscopic Evaluation of the Consciousness Energy Healing Treated Ashwagandha Root Extract

Ashwagandha (Withania somnifera) extract is a popular health supplement, used in many nutraceutical and pharmaceutical formulations for good health benefits along with the prevention and treatment of various human diseases. The purpose of this study was to evaluate the impact of The Trivedi Effect®-Consciousness Energy Healing Treatment on the characteristic properties of the phyto-compounds present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts, one part of the sample was termed as untreated/control sample, while the other part of the sample received The Trivedi Effect®-Biofield Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and termed as Biofield Energy Treated sample. The LC-ESI-MS data revealed that the chromatographic peak area% of the phytoconstituents present in the treated sample was significantly altered in the range of 31.13% to 20.61% compared to the control sample. Similarly, the average peak area of the treated sample was significantly improved by 20.61% compared to the control sample. A total of 14 withanolides such as viscosa lactone B, dihydrowithanolide D, withanolide A, withanolide B, etc. were proposed with m/z 489, 473, 471, and 455 at the retention times (Rt ) of 13.2, 14.5, 14.8, and 18.2 minutes, respectively. At Rt 13.2 minutes (m/z: 489), the peak area% and mass peak intensity of the phytocompound in the treated ashwagandha were significantly increased by 2.57% and 14.83%, respectively compared with the control sample. Similarly, at Rt 14.5 minutes (m/z: 473), peak area% was increased by 3.76%, but the mass peak intensity was significantly decreased by 26.48% in the treated sample compared with the control sample. At Rt 14.8 minutes (m/z: 471), peak area % was same, but the mass peak intensity was increased by 2.74% in the treated ashwagandha compared with the control sample. Subsequently, at Rt 18.2 minutes (m/z: 455), peak area% was increased 1.01%, whereas the peak intensity was significantly decreased by 10.90% in case of the treated sample compared to the control sample. These findings intensify to assume that The Trivedi Effect®-Consciousness Energy Healing Treatment might be responsible for the overall improvement of relative concentrations and altered relative abundance of the phytoconstituents in the ashwagandha root extract compared to the control sample. This Biofield Energy Treated ashwagandha root extract would be helpful to design better nutraceutical/pharmaceutical formulations against inflammatory diseases, immunological disorders, sexual disorders, arthritis, stress, cancer, diabetes, aging, and other chronic infections.

Isotopic Abundance Ratio Analysis of the Consciousness Energy Healing Treated Flutamide using LC-MS and GC-MS Spectrometry

Flutamide is used to treat prostate cancer, which acts by blocking the action of exogenous testosterone binding through its androgen receptor. This study was designed and performed to investigate the impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on the structural properties and the isotopic abundance ratio of flutamide using LC-MS and GC-MS spectroscopy. Flutamide sample was divided into control and treated parts. Only the treated flutamide received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a famous Biofield Energy Healer, Dahryn Trivedi. The LC-MS spectra of both the samples at retention time (Rt ) 3.4 minutes exhibited the mass of the deprotonated molecular ion peak at m/z 275.08 [M-H]-. The peak area of the treated sample was significantly increased by 92.42% compared to the control sample, which indicated that the solubility profile of the treated sample was significantly increased compared to the control sample. The LC-MS based isotopic abundance ratio of PM+1/PM (2 H/1 H or 13C/12C or 15N/14N or 17O/16O) in the treated flutamide was significantly increased by 59.48% compared with the control sample. The GC-MS based isotopic abundance ratio of PM+1/ PM in the treated flutamide sample was increased by 10.39% compared with the control sample. The results indicated that the 13C, 2 H, 15N, and 17O contributions from (C11H11F3 N2 O3 )+ to m/z 277 in the treated sample were significantly increased compared with the control sample. The isotopic abundance ratio of PM+1/PM (2 H/1 H or 13C/12C or 15N/14N or 17O/16O) in the treated flutamide was significantly increased compared to the control sample. The changes in the peak area and isotopic abundance could be due to changes in nuclei possibly through the interference of neutrino via the Trivedi Effect®-Consciousness Energy Healing Treatment. The new form of treated flutamide was formed that might have increase the chemical bond strength, stability, solubility, bioavailability, and efficacy, which could be very much useful to design more efficacious pharmaceutical formulations against prostate cancer, androgen-dependent skin and hair conditions including acne, seborrhea, hirsutism, and scalp hair loss, hyperandrogenism, as well useful for feminizing hormone therapy aimed at transgender women.

Assessment of Oral Bioavailability of Vitamin D 3 and Its Metabolite, 25-hydroxy (OH) Vitamin D 3 in Male Sprague Dawley Rats Following a Single Oral Dose of Biofield Energy Treated Vitamin D 3

The present study was performed to determine the effects of The Trivedi Effect ® -Energy of Consciousness Healing Treatment on vitamin D 3 and rats through the pharmacokinetic parameters measurement of both plasma level of vitamin D 3 and 25-hydroxy (OH)-vitamin D 3 after the oral administration of vitamin D 3 in rats. The test item, vitamin D 3 was divided into two parts. One part was denoted as the control (without Biofield Energy Healing Treatment), while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mahendra Kumar Trivedi. Additionally, one group of animals also received Consciousness Energy Healing Treatment per se by the Healer under similar conditions. Vitamin D 3 oral formulations were administrated by oral gavage at a dose of 500 μg per kg in groups viz. G1 (untreated Vitamin D 3 ), G2 (Biofield Energy Treated Vitamin D 3 ) and G3 (Biofield Energy Treated animals received untreated Vitamin D 3 ) group. The results showed that the relative oral exposure (AUC 0 –t) of vitamin D 3 was significantly increased by 155.62% and 111.73% in G2 and G3 groups, respectively as compared to the control group. Biofield Energy Treatment also significantly improved the plasma peak concentration (C max ) of vitamin D 3 by 223.84% and 187.5% in G2 and G3 groups, respectively as compared to the control group. This study results also showed that the relative oral exposure (AUC 0 –t) of 25-hydroxy (OH)-vitamin D 3 (metabolite) was significantly increased by 161.28% and 113.96% in G2 and G3 groups, respectively as compared to the control group (G1). Biofield Energy Treatment also significantly improved the plasma peak concentration (C max ) of 25-hydroxy (OH)-vitamin D 3 by 174.25% and 111.77% in G2 and G3 groups, respectively as compared to the control group. After oral administration, plasma concentrations of active metabolite, 25-hydroxy (OH)-vitamin D 3 in all three groups were declined slowly. The mean residence time (MRT last ) and elimination rate constant (K el ) for this metabolite were unaltered in all three groups. These data suggest that Biofield Energy Treated vitamin D 3 might be influenced in vivo biological activity of vitamin D 3 due to increased plasma exposure (AUC) of both vitamin D 3 and metabolite level, 25-hydroxy (OH)-vitamin D 3 in rats. There was significant improvement (>100%) of relative oral bioavailability of both vitamin D 3 and 25-hydroxy (OH)-vitamin D 3 that might be due to the alteration of physicochemical properties and thermal properties of vitamin D 3 by the Biofield Energy Treatment. Biofield Energy Healing Treatment could be an innovative strategy that opens new avenues to overcome poorly absorbed pharmaceuticals/nutraceuticals/herbal extracts and can also improve the therapeutic performance of orally active molecules.