Conocarpus Lancifolius Research Articles

In Silico Efficacy of Oleic Acid, n-Hexanoic acid, and Epiglobulol Obtained from Conocarpus Lancifolius against Class D β-lactamases

Introduction: Antimicrobial resistance poses an escalating global challenge, compounded by the sluggish pace of new antibiotic discovery. Objectives: This study undertook an in silico analysis of phytochemicals derived from Conocarpus lancifolius, a local plant in the Jazan region of Saudi Arabia, to investigate potential antibacterial agents. Methods: The 3D crystallographic structure of OXA-48 was sourced from the RCSB protein databank (PDBID: 3HBR) and pre-processed. The Lib Dock algorithm facilitated the analysis of optimal binding orientations and potential interactions between compounds and the 3HBR. Results: The docking analysis outcome revealed Oleic acid as the most active compound and the binding mode exhibited that the -OH group of the terminal carboxylic acid moiety formed hydrogen bonds with Thr197 and Ala207, and the oxygen atom showed bonding with Lys116. Moreover, N-hexanoic acid also demonstrated promising binding interactions with the 3HBR, displaying hydrogen bond interactions with Lys208, Tyr211, and Ser70, along with hydrophobic alkyl interactions with Lys208 and Met115 at the protein's active sites. Unfavourable donor-donor and acceptor-acceptor bindings were also observed with Ser70 and Arg250 residues. Conclusions: The OXA-48 and NDM-1 predominate in countries of the Arabian Peninsula. Our findings suggest oleic acid as a promising candidate for combating OXA-48 β-lactamase, pending further assessment of its atomic structure via X-ray crystallography.

Beyond polemics: towards an understanding of the narratives around the desired and despised Conocarpus tree in Kuwait for better urban green infrastructure

Over the past three decades, Kuwait’s urban landscape has become dominated by the controversial Conocarpus lancifolius tree. This exotic tree is shrouded in a polemic discourse, oscillating between desiring or despising it. On one extreme it is desired by local environmental activists as a versatile evergreen tree that can withstand Kuwait’s harsh arid climate and saline soil; on the other, it is despised by local ecologists and engineers for its monoculture and the destructive behaviour of its roots to subsurface urban infrastructure. This qualitative research aims to understand the multiple narratives around C. lancifolius by analysing institutional reports, social media, local news and grounded interviews. It argues for the need to move beyond polemics regarding C. lancifolius by calling for inclusive landscape governance frameworks for its management, and offers initial context-specific recommendations which may contribute to better urban green infrastructure (UGI) implementation in Kuwait.

Qualitative and quantitative phytochemical screening and chemical fingerprint analysis of Conocarpus lancifolius plant using HPTLC

Qualitative and quantitative phytochemical screening and chemical fingerprint analysis of Conocarpus lancifolius plant using HPTLC

Effect of Different Types of cuttings and Indole-3-Butyric Acid (IBA) on Rooting of Damas Plant (Conocarpus lancifolius L.)

An experiment consists of two types of cuttings viz. tip cutting (C1) and semi hardwood cuttings (C2) with different IBA concentrations i.e. 300 (I1), 400 (I2), 500 (I3) and 600 (I4) ppm each along with control (I5) were treated. The experiment was laid out in a Randomized Block Design with Factorial Concept comprise three replications. The cuttings were planted in sand bed. Among different types of cuttings, maximum number of roots (11.03), root length (6.99 cm), fresh weight (255.60 mg/plant) of root and dry weight (27.59 mg/plant) of root along with highest rooting percent (67.14) of cuttings were recorded in tip cutting after 60 days of planting. While in case of IBA, highest number of roots (13.23), longest root (7.66 cm), maximum fresh weight (294.33 mg/plant) of root, dry weight (27.59 mg/plant) of root and maximum rooting percentage (67.14) of cuttings were recorded under treatment of 500 ppm IBA at 60 days of planting. Interaction effect of tip cuttings along with 500 ppm IBA (C1I3) was also found significant for all the characters.

The Inhibitory Effect of Conocarpus Lancifolius Leaf Extract on Protease Produced by Clinical Pseudomonas Aeruginosa Isolate

The study was aimed at inhibiting the protease produced by Pseudomonas aeruginosa using an 80% alcoholic extract of Conocarpus lancifolius leaves. A total of 146 isolates of P. aeruginosa that were isolated and identified by microscopic and biochemical tests were 51 isolates submitted to primary and secondary screening techniques in order to choose the qualified P. aeruginosa isolate for protease synthesis. Among these isolates, forty-seven isolates showed hydrolysis zones on skim milk media (primary screening); six isolates were chosen for secondary screening. The result revealed that P. aeruginosa P51 had the highest ability to produce the enzyme, with a specific activity of 15.9 U/mg protein. In addition, the study included extracting the leaves of C. lancifolius using 80% ethanolic alcohol and conducting the GC-MC assay and the HPLC assay of the plant extract. The results revealed a significantly decreased specific activity of protease from 15.9 to 1.2 U/mg after treatment with 0.8 µg/ml of alcoholic extract of C. lancifolius leaves.

Conocarpus lancifolius Leaves as Low-Cost, Green and Sustainable Sorbent Material for Simultaneous Removal of Cadmium, Chromium, and Lead Ions from Aqueous Samples

Conocarpus lancifolius Leaves as Low-Cost, Green and Sustainable Sorbent Material for Simultaneous Removal of Cadmium, Chromium, and Lead Ions from Aqueous Samples

Evaluation of in vitro antioxidant, anti-inflammatory and antibacterial activities of chloroform leaf extracts of Conocarpus lancifolius Engl

Introduction and Aim: Antibacterial substances are abundant in medicinal herbs, which are used in many extremely effective traditional medical therapies. Conocarpus lancifolius Engl. a plant species, has a wide range of therapeutic uses for the handling of haemophilia, inflammation, illness, eye ailments, fever, diabetes and headache. The study's goal was to assess the antibacterial efficacy, in vitro antioxidant, anti-inflammatory properties of the solvent-based extract of C. lancifolius leaf material. Methodology: The investigation started in March 2023 and C. lancifolius leaves were collected in Tamil Nadu, India. To make the extracts, the maceration technique was employed. The phytochemical constitutions were identified by phytochemical test results. Using the agar diffusion method, the antibacterial capacity of C. lancifolius extracted from leaves was evaluated. Results: At the concentration of 100 microgram/mL level of probability, Gram-positive bacteria including Staphylococcus aureus in addition to Bacillus cereus, respectively (18 and 21 mm) and Gram-negative microorganisms Escherichia coli combined with Klebsiella pneumoniae (23 and 20 mm) were considerably inhibited. Additionally, it has the strongest antifungal activity (20 mm) against Candida albicans. While the ethanol extract of the leaves significantly inhibited both Gram-negative bacteria like Escherichia coli as well as the pneumonia-causing bacteria Klebsiella pneumoniae (22 and 19 mm) and Gram-positive bacteria like Staphylococcus aureus and the yeast Bacillus cereus (18 and 20 mm). Conclusion: When compared to normal ascorbic acid, which had an antioxidant activity of 87.96 percent, C. lncifolius extract with chloroform exhibited a substantial antioxidant activity of 62.96 percent inhibition. When compared to a larger dose of normal diclofenac (84.11 percent), the more potent quantity of C. lncifolius extract with chloroform significantly inhibited inflammation by 75.12 percent under similar circumstances. Because there are biologically active compounds in the herbal preparation, the current investigation found that C. lancifolius may be a good therapeutic candidate.

Conocarpus lancifolius (Combretaceae): Pharmacological Effects, LC-ESI-MS/MS Profiling and In Silico Attributes.

In folklore medicine, Conocarpus lancifolius is used to treat various illnesses. The main objective of this study was a comprehensive investigation of Conocarpus lancifolius leaf aqueous extract (CLAE) for its antioxidant, cardioprotective, anxiolytic, antidepressant and memory-enhancing capabilities by using different in vitro, in vivo and in silico models. The in vitro experimentation revealed that CLAE consumed an ample amount of total phenolics (67.70 ± 0.15 µg GAE/mg) and flavonoids (47.54 ± 0.45 µg QE/mg) with stronger antiradical effects through DPPH (IC50 = 16.66 ± 0.42 µg/mL), TAC (77.33 ± 0.41 µg AAE/mg) and TRP (79.11 ± 0.67 µg GAE/mg) assays. The extract also displayed suitable acetylcholinesterase (AChE) inhibitory (IC50 = 110.13 ± 1.71 µg/mL) activity through a modified Ellman's method. The toxicology examination presented no mortality or any signs of clinical toxicity in both single-dose and repeated-dose tests. In line with the cardioprotective study, the pretreatment of CLAE was found to be effective in relieving the isoproterenol (ISO)-induced myocardial injury in rats by normalizing the heart weight index, serum cardiac biomarkers, lipid profile and various histopathological variations. In the noise-stress-induced model for behavior attributes, the results demonstrated that CLAE has the tendency to increase the time spent in the central zone and elevated open arms in the open field and elevated plus maze tests (examined for anxiety assessment), reduced periods of immobility in the forced swimming test (for depression) and improved recognition and working memory in the novel object recognition and Morris water maze tests, respectively. Moreover, the LC-ESI-MS/MS profiling predicted 53 phytocompounds in CLAE. The drug-likeness and ADMET analysis exhibited that the majority of the identified compounds have reasonable physicochemical and pharmacokinetic profiles. The co-expression of molecular docking and network analysis indicated that top-ranked CLAE phytoconstituents act efficiently against the key proteins and target multiple signaling pathways to exert its cardiovascular-protectant, anxiolytic, antidepressant and memory-enhancing activity. Hence, this artifact illustrates that the observed biological properties of CLAE elucidate its significance as a sustainable source of bioactive phytochemicals, which appears to be advantageous for pursuing further studies for the development of new therapeutic agents of desired interest.

Potential plant leaves as sustainable green coagulant for turbidity removal

Chemical coagulation–flocculation has been used widely in water and wastewater treatment. In the present study, green coagulant was investigated. The role of Iraqi plants was examined to remove turbidity by using kaolin synthetic water. Thirteen selected plants were prepared as powdered coagulant. The experiment was run based on coagulant mass varied from 0 to 10,000 mg/L for each plant with a rapid mixing speed of 180 rpm for 5 min, slow mixing speed at 50 rpm for 15 min and settling time for 30 min. The seven best green coagulants are Albizia lebbeck (L.), Clerodendrum inerme (10,000 mg/L), Azadirachta indica, Conocarpus lancifolius, Phoenix dactylifera (5000 mg/L), Dianthus caryophyllus (3000 mg/L) and Nerium oleander (1000 mg/L) with turbidity removal rates of 39.3%, 51.9%, 67.2%, 75.5%, 51.0%, 52.6% and 57.2%, respectively. The selected seven plants that were used as green coagulants are economically feasible to achieve the highest turbidity and removal of other compounds.

New Hostplant Record for Lac Insect <i>Kerria</i> spp. from Andhra Pradesh

Lac is the only natural resin of animal origin produced by lac insects. The host range of lac insect is more than 400 plant species. Thick dark brown lac encrustation on the stem of an exotic plant Conocarpus lancifolius Engl., was noticed and recorded as a new host plant for lac insect from Andhra Pradesh, India.

Antimicrobial and antiquorum-sensing activity of Conocarpus lancifolius Engl. (Combretaceae)

Multidrug resistance to antimicrobial agents is a rapidly increasing problem. New strategies are required to reduce microbial resistance and pathogenicity. These include inhibiting microbial virulence factors, such as quorum-sensing (QS). In this study, the leaf and fruit extracts of Conocarpus lancifolius Engl. were investigated for their antimicrobial and QS inhibitory activities. The ethyl acetate extract of the leaf (EAL) recorded a remarkable inhibition of the Gram-negative bacteria; Escherichia coli and Klebsiella pneumonia (23 and 20 mm, respectively), using the agar well diffusion assay. Also, it showed the highest antifungal effect against Candida albicans (20 mm). Meanwhile, the ethyl acetate extract of the fruit (EAF) showed significant inhibition of the Gram-positive bacteria; Staphylococcus aureus and Bacillus cereus (20 and 21 mm, respectively). Measurement of the minimum inhibitory concentration (MIC) by the broth microdilution method indicated that Gram-positive bacteria were more susceptible to the antimicrobial effect of the investigated extracts. Particularly, EAL and EAF showed comparable activity to ampicillin against B. cereus (MIC, 312.5 µg/mL). The highest antifungal activity was recorded for the petroleum ether extract of the leaf (MIC, 625 µg/mL). Measurement of the inhibition diameter of pigment (PID) produced by the reporter strain, Chromobacterium violaceum indicated that EAL demonstrated higher QS inhibition (6 mm) compared to the standard, catechin (4.8 mm). In addition, the leaf and fruit extracts showed comparable activity to catechin (5 mm). The current study reported that C. lancifolius could be a promising antimicrobial and anti-quorum sensing drug candidate.

The Role of the Vegetative Part in Some Plant Species to Uptake and Accumulate Lead Element from Polluted Air (an Applied Study in Baghdad /Karkh in Iraq)

The current study was conducted to test the efficiency of the vegetative part (plant leaves) of plant species of shrubs and trees involved in forming semi-artificial vegetation in the city of Baghdad, Karkh, in the uptake and accumulating the lead element that pollutes the air in the city atmosphere. Five plant sampling sites were selected: Al-Kadhimiyah, Al-Mansour, Al-Ma'aml (Al-Salam district), Al-Adl, and Al-Ameriya district intersections (Al-Seklat), and symbols were given (A, B, C, D, E) respectively. The spread and distribution of plants vary in terms of human activities and pollution levels, affecting the five sites that recorded more than 20 species. For a real comparison between plant efficiency and the effect of the nature of the region, species of recurrent and non-recurring shrubs and trees were selected in their presence at the study sites and included (Conocarpus lancifolius, Ziziphus spina christi, Eucalyptus sp., Albizia lebbeck), and non-recurring (Nerium oleander, Dodonaea viscosa, Phoenix dactylifera, Olea europaea, Myrtus communis, Ficus nitida, Citrus aurantium). The study's results showed a variation in the ability of plant species in lead accumulators. The first site of the plants (Conocarpus lancifolius, Ziziphus spina christi, Albizia lebbeck, Eucalyptus sp., Nerium oleander, Dodonaea viscosa, Phoenix dactylifera, Olea europaea, Myrtus communis, Ficus nitida, and Citrus aurantium) was recorded at (0.46, 0.56, 0.36, 0.55, -, 0.68, -, -, 0.33, 0.29, 0.84) respectively. The second site of the same plants was (0.74, below the detection limit, 0.25, 0.57, -, -, 0.16, -, -, 0.31, -) respectively. The third site was (0.95, 0.65, 0.832, 0.831, 0.86, 1.02, -, -, -, 0.436, -, 0.532), respectively. The fourth site was (0.34, 0.95, 0.48, 0.40, -, 0.19, -, -, -, -), respectively. The fifth site was (0.48, 0.50, 0.49, 0.41, -, -, -, 1.45, -, -, -, -) ppm, respectively. The current study was conducted from October 2021 to May 2022

Spectral analysis, in vitro cytotoxicity and antibacterial studies of bioactive principles from the leaves of Conocarpus lancifolius, a common tree of Jazan, Saudi Arabia.

The objective of the present study was to analyse the bioactive compounds of the leaves of Conocarpus lancifolius (C. lancifolius). The GC-MS analysis of the hot methanolic extract of the leaves (HMEL) of C. lancifolius exhibited the bioactive compounds such as 1-(3-Methoxy-2-nitrobenzyl) iso quinoline, morphin-4-ol-6,7-dione, 1-bromo-N-methyl-, phytol, hexadecanoic acid, 2,3-dihydroxypropyl ester, 2,2':4',2"-terthiophene, ethyl iso-allocholate, caryophyllene oxide, campesterol, epiglobulol, cholestan-3-ol, 2-methylene-, (3á,5à)-, dasycarpidan-1-methanol, acetate (ester) and oleic acid, eicosyl ester. The FT-IR analysis of HMEL of C. lancifolius showed a unique peak at 3184, 2413, 1657 cm-1 representing coumaric acid, chlorogenic acid and ferulic acid. The HMEL of C. lancifolius was actively inhibiting the proliferation of breast cancer cells MCF-7 ATCC at the concentration of 72.66 ± 8.21 µg/ml as IC50 value. The HMEL of C. lancifolius also revealed a good spectrum of activity against Gram-positive and Gram-negative bacterial cultures screened in this work. The activity observed has shown more or less similar effects against screened bacteria. However, the magnitude of potentiality was significantly lesser compared to standard ciprofloxacin disc at p< 0.001 level (99% confidence intervals). Furthermore, the study demonstrating the bioactive compounds can be isolated from the leaves of C. lancifolius.

Molecular Modeling Study of Novel Lancifolamide Bioactive Molecule as an Inhibitor of Acetylcholinesterase (AChE), Herpes Simplex Virus (HSV-1), and Anti-proliferative Proteins.

Combretaceae, an immense family involving species (500) or genera (20), originates in tropical and subtropical regions. This family has evinced medicinal values such as anti-leishmanial, cytotoxic, antibacterial, antidiabetic, antiprotozoal, and antifungal properties. Conocarpus lancifolius (C. lancifolius) methanol extract (CLM) was prepared, then compound isolation performed by open column chromatography, and compound structure was determined by spectroscopic techniques (13C NMR, IR spectroscopy, 1H-NMR, mass spectrometry UV-visible, and 2D correlation techniques). Molecular docking studies of ligand were performed on transcriptional regulators 4EY7 and 2GV9 to observe possible interactions. Phytochemical screening revealed the presence of secondary metabolites including steroids, cardiac glycosides, saponins, anthraquinones, and flavonoids. The isolated compound was distinguished as lancifolamide (LFD). It showed cytotoxic activity against human breast cancer, murine lymphocytic leukemia, and normal cells, human embryonic kidney cells, and rat glioma cells with IC50 values of 0.72 µg/mL, 2.01 µg/mL, 1.55 µg/mL, and 2.40 µg/mL, respectively. Although no cytotoxic activity was noticed against human colon cancer and human lung cancer, LFD showed 24.04% inhibition against BChE and 60.30% inhibition against AChE and is therefore beneficial for Alzheimer’s disease (AD). AChE and LFD interact mechanistically in a way that is optimum for neurodegenerative disorders, according to molecular docking studies. Methanol and dichloromethane extract of C. lancifolius and LFD shows antibacterial and antifungal activity against antibiotic resistance Bacillus subtilis, Streptococcus mutans, Brevibacillus laterosporus, Salmonella Typhi, Candida albicans, and Cryptococcus neoformans, respectively. LFD shows antiviral activity against HSV-1 with 26% inhibition IP. The outcomes of this study support the use of LFD for cognitive disorders and highlight its underlying mechanism, targeting AChE, DNA-POL, NF-KB, and TNF-α, etc., for the first time.

INSECTICIDAL AND ANTIFEEDANT ACTIVITIES OF AQUEOUS LEAF EXTRACTS AGAINST Epilachna vigintioctopunctata (COCCINELLIDAE: COLEOPTERA) UNDER LABORATORY CONDITIONS

The aqueous leaf extracts of different plant such as Cryptostegia grandiflora, Mansoa alliacea, Allamanda cathartica, Allamanda blanchetti and Xanthium strumarium and leaves of tree species such as Kigelia pinnata, Bauhinia Purpurea, Drypetes roxburghi, Conocarpus lancifolius and Polyalthia longifolia were studied against the grubs of Epilachna vigintioctopunctata at various concentrations under laboratory conditions. Among the plant tested, the maximum insecticidal activity and leaf area protection was noticed in Xanthium strumarium 93.33% and 91.62% at 7% concentration with an estimated LC50 of 1.11% followed by D. roxburghii (80.00% &amp; 85.55%) and C. lancifolius (73.33% and 82.90%) respectively. The minimum per cent mortality was noticed in the treatment, Mansoa alliacea 7% with 40.00% and its antifeedancy were 50.97%. Results revealed that both the antifeedancy and mortality was increased with increasing in concentration of the plant extracts.

Biochemical changes in Conocarpus species under saline soils of Lal Suhanra National Park, Bahawalpur

In Pakistan, arid and semi-arid areas are more prone to limited rainfall, extensive evapo-transpiration and higher temperatures. For better understandings of current situation, a field experiment was carried out to study the morphological characteristics, biochemical responses and ionic composition of Conocarpus species (Conocarpus erectus and Conocarpus lancifolius) under saline soil conditions in Lal Suhanra National Park, Bahawalpur during 2019-21. Three sites one at normal soil (S.I) and two at different salinity levels (Medium and High Salinity) were observed in order to assess their effect on plant growth and other parameters. The data regarding physiological and biochemical parameters were recorded. Conocarpus lancifolius and Conocarpus erectus has maximum (233) mmol m-2sec-1 and (162) mmol m-2sec-1 stomatal conductance respectively. Maximum transpiration rate (4.57 MMOL M-2S-1) was observed at site-I in case of C. lancifolius, while maximum transpiration rate in case of C. erectus was (2.94 MMOL M-2S-1) at site-I. At control level, maximum photosynthetic rate was measured as (8.76 µmol m-2sec-1) in C. lancifolius and (5.59 µmol m-2sec-1) in case of C. erectus. Conocarpus species; Conocarpus lancifolius and Conocarpus erectus has maximum SOD (13.29 and 19.62) and CAT (16.48 and 42.05), and POD (14.81 and 8.81 U/mg protein) respectively. Maximum values of Na+K+ ratio in leaves (3.08), shoots (5.98) and roots (9.84) were detected at site-I in C. lancifolius. Based on statistically analyzed data, it is revealed that Conocarpus lancifolius can tolerate better salt stress as compared to Conocarpus erectus. Both species of Conocarpus can tolerate salinity up to 40 dSm-1 but growth of Conocarpus erectus is affected more as compared to Conocarpus lancifolius.

In vitro pharmacological attributes and metabolite’s fingerprinting of Conocarpus lancifolius

Search for safe antioxidants and novel nutraceuticals urged to evaluate the antioxidant, anti-acetylcholine esterase and anti-lipoxygenase activity of various leaf extracts of Conocarpus lancifolius. Extraction was optimized from freeze dried plant extracts quenched with liquid nitrogen using water, ethanol, methanol, hexane, ethyl acetate and chloroform. Maximum extract yield, total phenolic contents and total flavonoid contents were obtained in case of ethanolic extraction. The highest 2,2-diphenyl-1-picrylhydrazylradical scavenging in terms of IC50 value of 55.26 µg/mL was observed for ethanolic leaf extract. The acetylcholine esterase and lipoxygenase inhibitory activities (IC50) were also observed for ethanolic extract. These findings for ethanolic extract were statistically significant when compared with other extracts (ρ&lt;0.05). The haemolytic % values indicated that all extracts were associated with very low or negligible toxicity. The epicatechin, isorhamnetin, rutin, scopoleptin, skimmianine, quercetin-3-O-α-rhamnoside, quercetin-3-O-β-glucoside, cornoside, creatinine, choline, pyruvic acid, α-hydroxybutyric acid, phyllanthin and hypophyllanthin were identified as major functional metabolites in ethanolic leaf extract of C. lancifolius by 1H-NMR. The identified metabolites were probably responsible for the pharmacological properties of C. lancifolius. The findings may be utilized as pharmacological leads for drug development and food fortification.

Evaluating the Potential Use of Four Tree Species in the Greenbelts to Mitigate the Cement Air Pollution in Egypt

Air pollution due to industrial emissions is one of the environmental problems in the world. It has negative effects on the environment and the health of living organisms. Tree species can improve the air quality and aesthetic value of the landscape. They are affected by different air pollutants and show varying levels of tolerance and sensitivity when growing as green belts around cement factories. The present study aimed at evaluating the potential use of four tree species (Eucalyptus camaldulensis, Casaurina equisetifolia, Conocarpus lancifolius, Ficus benjamina) growing in the green belt of 21 cement factories in Egypt to mitigate air pollution through assessing their air pollution tolerance index (APTI) and anticipated performance index (API), compared with two reference sites. Fresh fully expanded leaves were collected from the selected species at the same time for laboratory measurements of leaf relative water content (RWC), ascorbic acid (AA), total chlorophyll (TCH), and leaf extract pH to calculate the APTI and API. The results showed that E. camaldulensis was anticipated as an excellent performer, C. equisetifolia and C. lancifolius as very good performers, while F. benjamina was anticipated as a good performer. The values of API for the target species indicated their tolerance to cement pollution. It is recommended that the target species be planted extensively in the green belts of cement factories and other urban industrial areas to mitigate cement air pollution.

Green synthesis of silver nanoparticles by Conocarpus Lancifolius plant extract and their antimicrobial and anticancer activities.

Due to drug addiction and the emergence of antibiotic resistance in pathogens, the disease load and medication intake have risen worldwide. The alternative treatment for drug-resistant infections is Nano formulation-based antimicrobial agents. The plant extract of Conocarpus Lancifolius fruits was used to synthesize silver nanoparticles in the current study, and it was further employed as an antimicrobial and anticancer agent. Nanoparticles have been characterized by UV–visible spectrometer revealed the notable peak of λmax = 410–442 nm, which confirms the reduction of silver ion to elemental silver nanoparticles, and the biological moieties in the synthesis were further confirmed by FTIR analysis. The stability and crystalline nature of materials were approved by XRD analysis and expected the size of the nanomaterials of 21 to 173 nm analyzed by a nanophox particle-size analyzer. In vitro, synthesized materials act as an antibacterial agent against Streptococcus pneumonia and Staphylococcus aureus. The inhibition zones of 18 and 24 mm have been estimated to be antibacterial activity against both bacteria. The potency of up to 100% of AgNPs for bacterial strains was incubated overnight at 60 μg/ml. Based on our results, biogenic AgNPs reveal significant activity against fungal pathogen Rhizopusus stolonifera and Aspergillus flavus that cause leading infectious diseases. Additionally, nanomaterials were biocompatible and demonstrated the potential anticancer activities against MDA MB-231 cells after 24-hour exposure.

Evaluation of the Antioxidant Activity of the Polyherbal (Conocarpus lancifolius L., Capparis spinosa L. and Dodonaea viscosa) Extracts and Assessment of the Hypoglycaemia Effect in Diabetic Mice

Diabetes mellitus, often referred to simply as diabetes, is a chronic metabolic disorder due to the relativedeficiency of insulin secretion and varying degrees of insulin resistance. It is characterised by high circulatingglucose. Excessive levels of either molecular oxygen or Reactive Oxygen Species (ROS) lead to an imbalancein the body’s normal oxidative metabolism that to leads to high glucose levels in the blood (hyperglycaemia),resulting in metabolic disturbances (oxidative stress) and chronic complications in diabetes. The presentstudy aims to estimate the antioxidant activity in diabetic mice induced via alloxan of a combination ofthree types of plant leaves (Conocarpus lancifolius L., Capparis spinosa L. and Dodonaea viscosa). Thetotal phenolic content using Folin-Ciocalteu reagent and the antioxidant activity utilizing 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay were estimated. The effect of polyherbal formulation leaves extracts onserum glucose level was done on forty-two albino mice divided into six groups and treated with polyherbalextracts and metformin. The results of total phenolic content in the polyherbal leaves extracts was observed(15.52 mg\g) in the aqueous extracted samples, while the total phenolics content was (46.97mg\g) in themethanolic extract, and the antioxidant activity showed the methanolic extract was the highest free radicalscavenging activity (93.28%) than the aqueous extract (86.77%) in 10 mg/ml and approach to the artificialantioxidant Butylated hydroxytoluene (BHT) which was (93.67%). This study showed both extracts ofpolyherbal leaves did not induce lethality in mice when administered orally at a dose of 2000 mg/kg. on theother hand, Diabetic mice treated with methanolic extract at doses 200 and 400 mg/kg showed a significantdecrease (p&lt;0.01) in serum glucose level after 35 days, which was 113.66 and 107.66 mg/dl respectively,and the aqueous extract was 124.66 and 117.00 mg/dl respectively when compared with the control positivegroup (324.00 mg/dl).