Conjugated Linoleic Acid Trans-11 Research Articles

Enhanced conjugated linoleic acid and biogas production after ruminal fermentation with Piper betle L. supplementation

ABSTRACT: Piper betle L. is edible plant richer in polyphenols that might improve feed utilization in rumen diet. The objective of the present study was to investigate the effect of various Piper betle L. powder (PL) doses on in vitro rumen microorganisms, ruminal biogas and fermentation end-product production, and biohydrogenation including lipolysis-isomerization. The completely randomized design used five levels of PL supplementation (0, 25, 50, 75 and 100 mg DM) incubated with 400 mg of a basal substrate of Pangola hay and concentrate (50:50). The matrix compounds (g/kg DM) of 0.27 catechin, 0.11 rutin, 3.48 quercetin, 0.41 apigenin, 0.04 myricetin, 0.27 kaempferol, 0.76 eugenol and 0.22 caryophyllene derived from PL altered the fermentation pattern, with an increase in degradable nutrients and total volatile fatty acids and acetogenesis without shifting pH during fermentation. These values promoted in vitro gas production, with higher carbon dioxide and lower methane production. Although, hydrogen recovery from lipolysis-isomerization in biohydrogenation was limited, PL successfully promoted stearic acid (C18:0) accumulation by changing the biohydrogenation pathway of fatty acids, causing more C18:1 trans-11 rather than C18:2 trans-11, cis-15. Consequently, this resulted in more conjugated linoleic acid (CLA) cis-9, trans-11, CLA trans-10, cis-12 and CLA trans-11, cis-13. Enhanced PL supply increased total bacteria and fungal zoospores due to a reduction in rumen protozoa. In conclusion, our results demonstrated that PL is a feed additive with potential for ruminants, promising improved ruminal fermentation and biohydrogenation, while reducing methane production.

Replacing canola meal as the major protein source with wheat dried distillers’ grains alters omasal fatty acid flow and milk fatty acid composition in dairy cows

Chibisa, G. E., Christensen, D. A. and Mutsvangwa, T. 2013. Replacing canola meal as the major protein source with wheat dried distillers’ grains alters omasal fatty acid flow and milk fatty acid composition in dairy cows. Can. J. Anim. Sci. 93: 137–147. Wheat dried distillers’ grains with solubles (W-DDGS) has a high fat content and is now commonly fed to dairy cows in western Canada. The objective was to determine the effects of replacing canola meal (CM) with W-DDGS on omasal fatty acid (FA) flow and milk FA composition. Four ruminally-cannulated lactating dairy cows were used in a 4×4 Latin square design with 28-d periods. Cows were fed either a standard barley silage-based total mixed ration (TMR) (0% W-DDGS, control) or TMR containing 10, 15 and 20% W-DDGS, with W-DDGS replacing CM as the major protein source. The omasal flow of C18:1 trans-10, C18:1 trans-11, C18:2n-6, C18:3n-3, conjugated linoleic acid (CLA) cis-9, trans-11, and total CLA increased linearly with increasing levels of W-DDGS. There were quadratic changes in milk concentrations of total C18:2, C18:3n-3, total n-3 polyunsaturated fatty acid (PUFA), and total n-6 PUFA as the level of W-DDGS increased. Feeding increasing amounts of W-DDGS resulted in a linear increase in milk concentrations of CLA cis-9, trans-11, CLA trans-11, trans-13, and total CLA, whereas milk concentrations of C18:1 trans-11 tended to increase linearly when level of W-DDGS increased. Feeding increasing amounts of W-DDGS resulted in quadratic changes in milk concentrations of C18:2n-6 and C18:3n-3. In summary, feeding increasing amounts of W-DDGS increased milk concentrations of key FA like C18:2n-6, C18:3n-3, CLA cis-9, trans-11, and total CLA, which can potentially improve human health.

Effect of diet on the deposition of n-3 fatty acids, conjugated linoleic and C18:1trans fatty acid isomers in muscle lipids of German Holstein bulls.

This study examined the effects of feeding diets rich in either n-3 or n-6 polyunsaturated fatty acids (PUFA) on the fatty acid composition of longissimus muscle in beef bulls. Thirty-three German Holstein bulls were randomly allocated to either an indoor concentrate system or periods of pasture feeding (160 days) followed by a finishing period on a concentrate containing linseed to enhance the contents of n-3 PUFA and conjugated linoleic acids (CLA) in beef muscle. The relative proportion and concentration (mg/100 g fresh muscle) of n-3 fatty acids in the phospholipid and triglyceride fractions were significantly increased (p < or = 0.05) in muscle lipids of pasture-fed bulls. The pasture feeding affected the distribution of individual CLA isomers in the muscle lipids. The proportion of the most prominent isomer, CLA cis-9,trans-11, was decreased from 73.5 to 65.0% of total CLA in bulls fed on concentrate as compared to pasture. The second most abundant CLA isomers were CLA trans-7,cis-9 and CLA trans-11,cis-13 in bulls fed on concentrate and pasture, respectively. Diet had no effect on the concentration of C18:1 trans-11. In contrast, the concentration of the C18:1 trans-13/14, trans-15, and trans-16 isomers in the muscle lipids was up to two times higher in pasture-fed as compared to concentrate-fed bulls. Pasture feeding enhanced the concentration of n-3 fatty acids, but the diet had no effect on the concentration of CLA cis-9,trans-11.