Congo-Angola Margin Research Articles

Relative Abundance and Diversity of Bacterial Methanotrophs at the Oxic-Anoxic Interface of the Congo Deep-Sea Fan.

Sitting at ∼5,000 m water depth on the Congo-Angola margin and ∼760 km offshore of the West African coast, the recent lobe complex of the Congo deep-sea fan receives large amounts of fluvial sediments (3–5% organic carbon). This organic-rich sedimentation area harbors habitats with chemosynthetic communities similar to those of cold seeps. In this study, we investigated relative abundance, diversity and distribution of aerobic methane-oxidizing bacteria (MOB) communities at the oxic–anoxic interface of sedimentary habitats by using fluorescence in situ hybridization and comparative sequence analysis of particulate mono-oxygenase (pmoA) genes. Our findings revealed that sedimentary habitats of the recent lobe complex hosted type I and type II MOB cells and comparisons of pmoA community compositions showed variations among the different organic-rich habitats. Furthermore, the pmoA lineages were taxonomically more diverse compared to methane seep environments and were related to those found at cold seeps. Surprisingly, MOB phylogenetic lineages typical of terrestrial environments were observed at such water depth. In contrast, MOB cells or pmoA sequences were not detected at the previous lobe complex that is disconnected from the Congo River inputs.

First respiration estimates of cold-seep vesicomyid bivalves from in situ total oxygen uptake measurements

Vesicomyid bivalves are one of the most abundant symbiont-bearing species inhabiting deep-sea reducing ecosystems. Nevertheless, except for the hydrothermal vent clam Calyptogena magnifica, their metabolic rates have not been documented, and only assessed with ex situ experiments. In this study, gathering benthic chamber measurements and biomass estimation, we give the first in situ assessment of the respiration rate of these bivalves. The giant pockmark Regab, located at 3160m depth along the Congo-Angola margin, is a cold-seep site characterised by dense assemblages of two species of vesicomyids: Christineconcha regab and Laubiericoncha chuni with high dominance of C. regab. Two sites with dense aggregates of vesicomyids were selected to measure total oxygen uptake (TOU), and methane fluxes using IFREMER's benthic chamber CALMAR deployed by the ROV Quest 4000 (MARUM). Photographs were taken and bivalves were sampled using blade corers to estimate density and biomass. Total oxygen uptake was higher at Site 2 compared to Site 1 (respectively 492mmol.m−2.d−1 and 332mmol.m−2.d−1). However, given vesicomyid densities and biomass, mean oxygen consumption rates were similar at both sites (1.9 to 2.5μmol.g total dry mass−1.h−1 at the Site 1 and 1.8 to 2.3μmol.g total dry mass−1.h−1 at Site 2). These respiration rates are higher than published ex situ estimates for cold-seep or hydrothermal vent bivalves. Although methane fluxes at the base of sulphide production were clearly higher at Site 2 (14.6mmol.m−2.d−1) than at Site 1 (0.3mmol.m−2.d−1), they do not seem to influence the respiration rates of these bivalves associated to sulphide-oxidizing symbionts.

Influence of seep emission on the non-symbiont-bearing fauna and vagrant species at an active giant pockmark in the Gulf of Guinea (Congo–Angola margin)

Detailed surveying with an ROV found that a dense and diverse cold-seep community colonises a giant pockmark located at 3200 m depth, 8 km north from the deep Congo channel. Several types of assemblages, either dominated by Mytilidae and Vesicomyidae bivalves or Siboglinidae polychaetes, are distributed on the 800-m diameter active area. The site is characterised by a most active central zone in a depression with abundant carbonate concretions and high methane fluxes where high-density clusters of mussels and siboglinids dominate. In contrast, the peripheral zones display large fields of dead and live vesicomyids on soft sediment, with a lower mean density and lower methane concentration in seawater. The associated megafauna includes Alvinocarididae shrimps, echinoids, holothurians of the family Synaptidae, several species of gastropods, two species of galatheids, and Zoarcidae and Ophidiidae fishes. Multivariate analyses of video transect data show that the distribution of these major megafauna species at the pockmark scale is influenced by the habitat heterogeneity due to fluid or gas emission, occurrence of hydrates, substratum variability and by the presence of large symbiont-bearing species. Several assemblages dominated either by mytilids, vesicomyids, or siboglinids have been sampled for megafauna densities and biomass estimations and stable isotope measurements ( δ 13 C and δ 15 N) of dominant species and food sources. The highest estimates of megafauna densities have been obtained in mytilid beds. According to their stable isotopes values, non-symbiont-bearing species mainly rely on chemosynthesis-originated carbon, either as primary consumers of chemoautotrophic microorganisms, or at higher trophic level recycling organic matter, or relying on bivalve and tubeworm production. Most of them likely feed on different sources like shrimps, but differences according to habitat have been evidenced. Carbon and nitrogen isotope ratios of galatheids and benthic or benthopelagic fishes captured by trawls at increasing distances from the pockmark provide evidence of the high variability in the proportion of chemosynthesis-originated carbon in their diet, from 15% to 38%, according to the species captured as far as 4 km to the site.

Large Vesicomyidae (Mollusca: Bivalvia) from cold seeps in the Gulf of Guinea off the coasts of Gabon, Congo and northern Angola

Two new genera and three new species of large Vesicomyidae are described from cold-seep sites on pockmarks and other sulfide-rich environments in the Gulf of Guinea (tropical east Atlantic) off Gabon, Congo (Brazzaville) and northern Angola, from 500 to 4000 m depth: “Calyptogena” (s.l.) regab n. sp., Wareniconcha (n.g.) guineensis (Thiele and Jaeckel 1931), Elenaconcha guiness n.g. n. sp., and Isorropodon atalantae n. sp. For two other species already taken by the R/V Valdivia in 1898, Calyptogena valdiviae (Thiele and Jaeckel 1931) and Isorropodon striatum (Thiele and Jaeckel 1931) new localities were discovered, and the species are rediscussed. E. guiness n.g. n.sp. is also recorded from off Banc d’Arguin, Mauritania, collected by commercial fishing vessels. The vesicomyid species here treated were encountered in different depth ranges along the Gabon–Congo–Angola margin, between 500 and 4000 m depth, and it was found that, in comparison with the dredge samples taken by the Valdivia expedition off southern Cameroon and off Rio de Oro (both at 2500 m), the same species occur in other depth ranges, in some cases with a vertical difference of more than 1000 m. .That means that the species are not confined to a given depth thought being typical for them and that the characteristics of the biotope are likely to play a major role in the distribution of the vesicomyids associated to cold seeps or other reduced environments along the West African margin.

Geological overview of the Angola–Congo margin, the Congo deep-sea fan and its submarine valleys

The Congo deep-sea fan is one of the largest fans in the world still affected by presently active turbidity currents. The present activity of deep-sea sedimentary processes is linked to the existence of a direct connection between the Congo River estuary and the Congo canyon head that allows relatively continuous sediment feeding of the deep-sea environment, in spite of a wide continental shelf (150 km). Because of this important activity in terms of sedimentary processes, the deep-sea environment of the Congo-Angola margin presents major interests concerning physical, chemical and biological studies near the seafloor. The main aim of this paper is to present the initial geological context of the BioZaire Program, showing a synthesis of the major results of the ZaiAngo Project including (1) the brief geological setting of the Congo-Angola margin, (2) the structure of the modern Congo deep-sea fan, (3) the sedimentary architecture of the recent Congo turbidite system (from the canyon to the distal lobes) and (4) the recent and present turbidite sedimentation. In order to provide useful information and advice relevant to biological and geochemical studies across the Congo sedimentary system, this article is particularly focused on the present sedimentary processes and the present activity of turbidity current along the Congo canyon and channel.

The influence of Congo River discharges in the surface and deep layers of the Gulf of Guinea

The main feature of the Congo-Angola margin in the Gulf of Guinea is the Congo (ex-Zaire) deep-sea fan composed of a submarine canyon directly connected to the Congo River, a channel and a [sediment] lobe area. During the multi-disciplinary programme called BIOZAIRE conducted by Ifremer from 2000 to 2005, two CTD-O2 sections with discrete water column samples were performed (BIOZAIRE3 cruise: 2003–2004) to study the influence of the Congo River discharges, both in the surface layer and in the deep and near-bottom layers. The surface layer water is greatly diluted with river water that has a heavy particle load. The deep layer is affected by episodic turbidity currents that flow in the deep Congo channel and reach deep areas far from the coast. Previous studies revealed deep anomalies in oxygen (deficit) and nutrient (excess) concentrations at not, vert, similar4000 m depth and assumed that they resulted from mineralisation of the particulate organic matter from the Congo River. The BIOZAIRE3 sections were designed to explore these phenomena in more detail near the Congo channel. Oxygen and nutrients were measured as well as additional parameters, including stable isotopes of oxygen and carbon, dissolved inorganic carbon and pH. For the surface layer, the effect of the Congo River was studied with reference to salinity. Deviations from the theoretical dilution of various inorganic solutes suggested the occurrence of mineralisation and consumption processes. For the deep layer, the network of CTD-O2 stations gave a more detailed description of the deep anomalies than in previous studies. From the east–west section, anomalies appeared on the bottom at 4000 m depth and became slightly shallower when they spread to the west. They were also present north and south on the bottom along the 4000 m isobath. In these deep waters, the decrease in the δ13C values of dissolved inorganic carbon confirmed that the mineralisation of organic matter plays a role in generating these anomalies. The location of the origin of this deep anomaly is debated. Here, arguments are given in favour of mineralisation of the particulate organic matter input that overflows from the Congo channel at not, vert, similar4000 m depth during turbidity current events. Other authors suggest that this input comes from downslope particle transport. Anomalies of the same origin, but weaker, also occurred deeper on the Congo lobe, where the Congo channel ends, but with a significant pH decrease on the bottom which was not seen at 4000 m depth.

Geological constraints on the evolution of the Angolan margin based on reflection and refraction seismic data (ZaïAngo project)

SUMMARY Deep penetration multichannel reflection and Ocean Bottom Seismometer wide-angle seismic data from the Congo-Angola margin were collected in 2000 during the Za¨ iAngo cruise. These data help constrain the deep structure of the continental margin, the geometry of the pre-salt sediment layers and the geometry of the Aptian salt layer. Dating the deposition of the salt relative to the chronology of the margin formation is an issue of fundamental importance for reconstructing the evolution of the margin and for the understanding of the crustal thinning processes. The data show that the crust thins abruptly, from a 30-40 km thickness to less than 10 km, over a lateral distance of less than 50 km. The transitional domain is a 180-km-wide basin. The pre-salt sediment layering within this basin is parallel to the base of the salt and hardly affected by tectonic deformation. In addition, the presence of a continuous salt cover, from the continental platform down to the presumed oceanic boundary, provides indications on the conditions of salt deposition that constrain the geometry of the margin at that time. These crucial observations imply shallow deposition environments during the rifting and suggest that vertical motions prevailed—compared to horizontal motions—during the formation of the basin.

Physical and chemical characterization of gas hydrates and associated methane plumes in the Congo–Angola Basin

As a part of the ZAIANGO IFREMER/TOTAL-FINA-ELF program, gas hydrates were collected from a gravity sediment core on the Congo–Angola margin during the ZAI-ROV cruise (December 2000). Gas hydrates, associated with a deep giant (800 m in diameter) active depression called “pockmark,” occurred from the sediment surface down to 12 m depth, at 3160 m water depth. Elevated concentrations of particles, manganese, iron, and methane detected in the water column close to the sediment surface reveal intense fluid circulation transporting methane-rich turbid fluids into the overlying bottom waters. Free gas is liberated via diffusion and/or advection and from destabilizing gas hydrates. The gas hydrates occur as small fragments and massive crystal aggregates, mostly disseminated irregularly in the highly disturbed sediment and escaping in the overlying deep seawater, creating methane-rich plumes. The dissociation of solid CH 4 hydrate particles rising in a turbulent flow may explain the high heterogeneity of methane concentration and CH 4 peaks measured in vertical profiles. Raman spectroscopy revealed that the gas hydrates recovered from the Congo–Angola are mainly 100% methane gas hydrate of structure I cubic with a lattice constant of a=12 A°, but H 2S and CO 2 are co-clathrated with CH 4 in cages. Analyses of hydrate water show depletions of Cl, SO 4, Na, Mg, Ca, and Sr, and enrichments of Si, Cs, and Ba, compared to the ambient deep seawater. Gas analysis shows that methane is the major component (99.1%), but CO 2 (0.83%) and heavier gases such as C 2H 6 (0.043%) and H 2S (0.02%) are also present as traces. Helium concentrations were in the range of 0.04–1.3 ppm, with slightly radiogenic 3He/ 4He ratios between 6.6×10 −7 and 7.6×10 −7. The hydrate methane has δ 13C of −69.3‰ (PDB) and δ D of −199‰ (V-SMOW), and the hydrate CO 2 has δ 13C of −17.5‰ (PDB). These values indicate a primarily microbial origin for the CH 4, which is generated through bacterial CO 2 reduction, as previously observed on many continental margins where solid gas hydrates were sampled.

Architecture of an active mud-rich turbidite system: The Zaire Fan (Congo–Angola margin southeast Atlantic)

Multichannel seismic data newly acquired during two ZaAngo surveys now provide an almost complete view of the Quaternary architecture of the Zaire Fan. Extending laterally from the southern Gabon margin to the Angola margin and longitudinally more than 800 km, the overall fan consists of three main individual fans that were deposited successively as overlapping depocenters. The individual fans are composed of channel/levee systems exhibiting similar seismic facies, external configurations, and organization to those described in other large mud-rich systems (e.g., the Amazon Fan). In particular, high-amplitude reflection units with a high oil-reservoir potential are recognized almost systematically as a basal sole for channel/levee systems. They possibly include true high-amplitude reflection packets related to avulsion processes below the avulsion points and coarse-grained basal levees related to the initial stages of levee aggradation subsequent to the avulsion. Correlations with Ocean Drilling Program Leg 175 Site 1077 indicate that the studied part of the Zaire Fan began to build in the late Pleistocene (780 ka). During the upper Quaternary, a great number of channel/levee systems (more than 80) were developed, possibly explained either by its permanent activity even during high sea level conditions or by the low Zaire River inputs. The frequent occurrence of channel entrenchment of either old or recent channels is another characteristic specific to the fan. Overdeepening of channels is probably partly caused by regressive erosion inside the parent channel in response to an avulsion and also in part because of other causes that are not fully understood.