Control Condition Research Articles

Feet-heating and Calf-heating have Opposing Effects on Glucose Tolerance and Heart Rate Variability: A Randomized, Controlled, Crossover Trial.

Heat exposure's effect on glucose tolerance depending on the amount of body exposure, likely relating to autonomic nervous system balance. We assessed how partial-body heat exposure at two different levels of the lower extremities would affect glucose tolerance and autonomic nervous system balance measured via heart rate variability. We hypothesized feet-heating would improve glucose tolerance without affecting heart rate variability while calf-heating would worsen glucose tolerance and decrease heart rate variability compared to a thermoneutral control condition. In a randomized, controlled, crossover trial, healthy participants' (N=31, 23(3)years, 45% male) glucose tolerance was measured in A)thermoneutral; B)feet-heating; and C)calf-heating conditions. Every 30 minutes for 2 hours, blood glucose, heart rate, heart rate variability, tympanic temperature, thermal comfort scores, and blood pressure were measured. There were significant interactions between condition and time for blood glucose (F(4.6,72.6)=2.6,p=.036), heart rate (F(3.4,54.5)=3.5,p=.017), heart rate variability (F(4.3,63.2)=7.5,p<.0001), tympanic temperature (F(8,268)=2.4,p=.014), and thermal comfort scores (F(8,248)=22.1,p<.0001). Calf-heating increased 90-minute glucose (+12(95%CI: 3 to 21)mg/dL,p=.013) and decreased heart rate variability throughout (mean decrease: 13-22%,p<.007), while feet-heating lowered 90-minute glucose (-7(95%CI:-16 to +1)mg/dL,p=.090) without affecting heart rate variability (p=.14-.99). Blood pressure and body temperature were similar between conditions, but heart rate and thermal comfort scores increased with heating. Calf-heating worsens, while feet-heating may improve, glucose tolerance. Changes in heart rate variability coincide with changes in glucose tolerance despite unchanged body temperature. Whether heart rate variability can be used to monitor autonomic nervous system balance during heating to the effect on acute glycemic indices should be further explored.

A Technology-Supported Psychoeducational Intervention for Older Adults and Their Families to Improve Social Isolation, Loneliness, and Intergenerational Connectedness-A Randomized Controlled Study.

Information and communication technologies (ICT) provide digital tools to support and facilitate social engagement. However, the extent to which new ICTs, such as instant messaging, and training on its use, enhance intergenerational family interactions and reduce social isolation and loneliness needs further exploration. In the present study, we developed a brief psychoeducational intervention to promote older adults and their family members (N = 64) to use new technologies to increase communication with each other. With a randomized controlled trial design, we evaluated the effectiveness of the intervention in changing primarily participants' intergenerational interactions, perceived social isolation, and loneliness. Through repeated-measures analysis of variance, an overall condition by time effect was found in younger family members such that contact frequency improved in the intervention condition compared with the waitlist control condition. Using a dyadic framework, we further investigated the role of the intervention in fostering interactions among family members. Dyadic analyses showed a partner effect with contact frequency in younger adults at pre-assessment predicting contact frequency of older adults at post-assessment. This suggests that support and encouragement from younger family members could increase intergenerational interactions and technology adoption among older adults. Moreover, online phone calls served as a promising tool to enhance intergenerational communication; higher user satisfaction was associated with increased ICT use, perceived usefulness, and fewer barriers to technology. This study provides preliminary findings on the effectiveness of ICT-based interventions for engaging older adults and their family members in the use of newer technologies to promote intergenerational interactions and reduce social isolation.

When the attention control condition works: A systematic review of attention control training for posttraumatic stress disorder.

Attentional bias and deficits in attentional control are associated with posttraumatic stress disorder (PTSD) symptoms. Attention control training (ACT) may address these factors. We reviewed randomized controlled trials (RCTs) of ACT for PTSD to address unanswered questions about ACT's effectiveness, tolerability, and implementation. Studies were included if they were an RCT that used an adult sample, recruited participants with a PTSD diagnosis, and had ACT as at least one treatment arm. The PTSD Trials Standardized Data Repository (PTSD-Repository) and additional databases were searched to identify PTSD RCTs published through May 2024. Seven studies met the inclusion criteria (N = 407). The effect size for ACT versus a comparison condition on PTSD symptoms was large, but the confidence interval (CI) overlapped with 0, g = 0.75, 95% CI [-0.63, 2.12]. The same pattern was observed for attention bias variability, g = 1.04, 95% CI [-0.90, 2.98]. There was a significant within-group effect of ACT on self-reported PTSD symptoms, g = -1.43, 95% CI [-2.83, -0.03]. Risk of bias varied, with high risk of bias being primarily due to bias in the measurement of the outcome. These effects should be interpreted cautiously given the significant heterogeneity and wide confidence intervals observed. It remains unclear for whom and under what conditions ACT may be most effective. Future studies should move beyond response time measures, employ an inactive comparator, and examine the mechanism of action to determine whether ACT could be a viable intervention for PTSD.

Multiplexed chromatin immunoprecipitation sequencing for quantitative study of histone modifications and chromatin factors.

ChIP-seq is a widely used technique for studying histone post-translational modifications and DNA-binding proteins. DNA fragments associated with a specific protein or histone modification epitope are captured by using antibodies, sequenced and mapped to a reference genome. Albeit versatile and popular, performing many parallel ChIP-seq experiments to compare different conditions, replicates and epitopes is laborious, is prone to experimental variation and does not allow quantitative comparisons unless adequate spike-in chromatin is included. We present a detailed protocol for performing and analyzing a multiplexed quantitative chromatin immunoprecipitation-sequencing experiment (MINUTE-ChIP), in which multiple samples are profiled against multiple epitopes in a single workflow. Multiplexing not only dramatically increases the throughput of ChIP-seq experiments (e.g., profiling 12 samples against multiple histone modifications or DNA-binding proteins in a single experiment), but also enables accurate quantitative comparisons. The protocol consists of four parts: sample preparation (i.e., lysis, chromatin fragmentation and barcoding of native or formaldehyde-fixed material), pooling and splitting of the barcoded chromatin into parallel immunoprecipitation reactions, preparation of next-generation sequencing libraries from input and immunoprecipitated DNA and data analysis using our dedicated analysis pipeline. This pipeline autonomously generates quantitatively scaled ChIP-seq tracks for downstream analysis and visualization, alongside necessary quality control indicators. The entire workflow requires basic knowledge in molecular biology and bioinformatics and can be completed in 1 week. MINUTE-ChIP empowers biologists to perform every ChIP-seq experiment with an appropriate number of replicates and control conditions, delivering more statistically robust, exquisitely quantitative and biologically meaningful results.

Distribution of Non-Structural Carbohydrates and Root Structure of Plantago lanceolata L. under Different Defoliation Frequencies and Intensities

Plantago lanceolata L. (plantain) increases herbage dry matter (DM) production and quality during warm and dry conditions due to its deep roots and drought tolerance and reduces nitrogen losses in grazing systems compared to traditional pastures. However, plantain density usually declines after the third growing season, mainly due to defoliation management. The effects of defoliation frequency and intensity on water-soluble carbohydrate (WSC) reserves and below-ground plant responses need further research to optimize grazing strategies for improved productivity and sustainability of grazing systems. Our study investigated the effects of defoliation frequencies (15, 25, and 35 cm of extended leaf length, ELL) and intensities (5 and 8 cm of residual heights) on morphological traits and WSC concentrations in plantain biomass under controlled environmental conditions. Defoliation frequency significantly influenced morphological and chemical characteristics and biomass distribution more than residual height. Less frequent defoliations promoted above-ground herbage DM production, reproductive stems, and root biomass. Root architecture showed adaptations in response to defoliation frequency, optimizing resource acquisition efficiency. Frequent defoliation reduced high molecular weight WSC concentrations in leaves, affecting regrowth capacity and DM mass. A defoliation frequency of 25 cm ELL (~15 days) balances herbage production and root development, promoting long-term pasture sustainability.

Effects of different pre-exercise strategies on jumping performance in female volleyball players.

The present study aimed to compare different pre-exercise strategies on jumping performance in female volleyball players. Fifteen healthy female volleyball players (age=18±0.6 years; training experience = 7.3±1.4 years; height = 164.8±5.4 cm; body mass = 57.2±8.1 kg) volunteered to participate in the study. Three different pre-exercise conditions (5 repetition maximum knee extension, electromyostimulation [EMS] and ischemic preconditioning [IPC]) were applied to the subjects and compared to a control condition performing a standardized warmup. Subjects performed the squat jump and 15 sec repeated countermovement jumps following a rest period. Measures associated with jumping performance were collected and compared between conditions. Rating of perceived exertion was also collected after each performance test. No pre-exercise condition outperformed a standardized warm-up on inducing improvements in jumping performance and in fact, EMS and IPC conditions resulted in performance decrements compared to control (P<0.05). We conclude that a standardized warm-up is enough to induce improvements in jumping performance in female volleyball players. Future research should examine alternative strategies alongside standardized warm-up to determine how best to prepare for jumping and related sport-specific tasks in female volleyball players.

Evaluating technology breaks on cell phone use in a college classroom

Cell phones in the college classroom can be used to increase interaction between students and the professor; they can also distract from academic tasks and decrease academic performance. To decrease task-switching in the classroom, researchers have suggested the use of “technology breaks” (TB), in which students are provided periodic breaks to use cell phones throughout class. The purpose of the present study was to evaluate the use of technology breaks in a college classroom (N = 21). Cell phone use was evaluated over 22 class periods. Observers recorded how many students were using cell phones every 10 s. Three experiment conditions were alternated with yoked controls in a multi-element design: (A) 1 min technology breaks, (B) 2 min technology break, and (C) 4 min technology break. The control condition [question breaks, (QB)] provided breaks for students to ask the professor questions regarding class materials. No penalties or punishers were delivered for cell phone use under any conditions. The average rate of cellphone use in QB was 0.53 responses per min (range = 0.06–1.02), while the average rate for TB was 0.35 responses per min (range = 0.20–0.74). Overall, the study found that technology breaks were a promising way to utilize reinforcement-based strategies to reduce classroom cell phone use, though variability in the data weakened conclusions regarding the utility of technology breaks.

Characteristics of High-Intensity Interval Training Influence Anthropometrics, Glycemic Control, and Cardiorespiratory Fitness in Type 2 Diabetes Mellitus: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Exercise is a non-pharmacological intervention for type 2 diabetes mellitus (T2DM), including moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT). Despite diverse exercise protocol variations, the impact of these variations in HIIT on T2DM anthropometrics, glycemic control, and cardiorespiratory fitness (CRF) remains unclear. The aim was to examine the influence of HIIT protocol characteristics on anthropometrics, glycemic control, and CRF in T2DM patients and compare it to control (without exercise) and MICT. This review is registered in PROSPERO (CRD42021281398) and follows Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The search, employing "high-intensity interval training" and "diabetes mellitus" in PubMed and Web of Science databases, with a "randomized controlled trial" filter, spanned articles up to January 2023. Of 190 records, 29 trials were included, categorized by HIIT interval duration, training volume, and intervention period. Long-duration, high-volume, and long-term HIIT yields superior outcomes compared to control conditions for body mass, waist circumference, fasting plasma glucose, Homeostatic Model Assessment for Insulin Resistance (HOMA-IR), glycosylated hemoglobin (%HbA1c), and CRF. The findings favored HIIT over MICT for body mass in long-duration, high-volume, and short-term intervals (mean difference[MD] - 3.45, - 3.13, and - 5.42, respectively, all p < 0.05) and for CRF in long and medium work intervals and high volume (MD 1.91, 2.55, and 2.43, respectively, all p < 0.05), as well as in medium and long-term intervention (MD 2.66 and 2.21, respectively, all p < 0.05). Regardless of specific HIIT characteristics, no differences were found in the HIIT versus MICT comparison for glycemic control. Specific HIIT protocol characteristics influence changes in anthropometrics, glycemic control, and CRF compared to control groups. However, compared to MICT, only longer duration, higher volume, and short-term HIIT improved body mass, waist circumference, and CRF in individuals with T2DM.

Differential Drought Responses of Soybean Genotypes in Relation to Photosynthesis and Growth-Yield Attributes

Water scarcity leads to significant ecological challenges for global farming production. Sustainable agriculture depends on developing strategies to overcome the impacts of drought on important crops, including soybean. In this present study, seven promising soybean genotypes were evaluated for their drought tolerance potential by exposing them to water deficit conditions. The control group was maintained at 100% field capacity (FC), while the drought-treated group was maintained at 50% FC on a volume/weight basis. This treatment was applied at the second trifoliate leaf stage and continued until maturity. Our results demonstrated that water shortage exerted negative impacts on soybean phenotypic traits, physiological and biochemical mechanisms, and yield output in comparison with normal conditions. Our results showed that genotype G00001 exhibited the highest leaf area plant−1 (483.70 cm2), photosynthetic attributes like stomatal conductance (gs) (0.15 mol H2O m−2 s−1) and photosynthetic rate (Pn) (13.73 μmol CO2 m−2 s−1), and xylem exudation rate (0.25 g h−1) under drought conditions. The G00001 genotype showed greater leaf greenness by preserving photosynthetic pigments (total chlorophylls (Chls) and carotenoids; 4.23 and 7.34 mg g−1 FW, respectively) in response to drought conditions. Soybean plants accumulated high levels of stress indicators like proline and malondialdehyde when subjected to drought stress. However, genotype G00001 displayed lower levels of proline (4.49 μg g−1 FW) and malondialdehyde (3.70 μmol g−1 FW), indicating that this genotype suffered from less oxidative stress induced by drought stress compared to the other investigated soybean genotypes. Eventually, the G00001 genotype had a greater yield in terms of seeds pod−1 (SP) (1.90) and 100-seed weight (HSW) (14.60 g) under drought conditions. On the other hand, BD2333 exhibited the largest decrease in plant height (37.10%), pod number plant−1 (85.90%), SP (56.20%), HSW (54.20%), gs (90.50%), Pn (71.00%), transpiration rate (59.40%), relative water content (34.40%), Chl a (79.50%), total Chls (72.70%), and carotenoids (56.70%), along with the maximum increase in water saturation deficit (290.40%) and malondialdehyde content (280.30%) under drought compared to control conditions, indicating its higher sensitivity to drought stress. Our findings suggest that G00001 is a promising candidate to consider for field trials and further evaluation of its molecular signature may help breeding other elite cultivars to develop drought-tolerant, high-yielding soybean varieties.

Analytical Solutions for a Nonlinear Quadratic Delayed Functional Integral Inclusion With Feedback Control on the Real Half-Line

Let E be a reflexive Banach space. In this research, we are interested in the solvability of the nonlinear quadratic delayed functional integral inclusion (NQDFII) with a feedback control condition on the real half-axis. Our examination is found within the space BC(R+, E) of bounded continuous functions on the real half-axis R+ and takes values in a reflexive Banach space E beneath the assumption that the set-valued function G satisfy Lipschitz condition in E. The base we depend on in this study is the procedure related to a measure of noncompactness in the space BC(R+, E) by a given norm of continuity and applying Darbo’s fixed point theorem. Moreover, the asymptotic stability of the solution and the asymptotic dependency of the solution on the set of selections SG will be examined. Also, we give an example to illustrate the adequacy and esteem of our comes about.

Physio-biochemical mechanism of melatonin seed priming in stimulating growth and drought tolerance in bread wheat

Drought stress (DS) adversely affects a plant’s development and growth by negatively altering the plant’s physio-biochemical functions. Previous investigations have illustrated that seed priming with growth regulators is an accessible, affordable, and effective practice to elevate a plant’s tolerance to drought stress. Melatonin (MT) is derived from the precursor tryptophan and can improve germination, biomass, and photosynthesis under stress conditions. The current study examined the effect of melatonin seed priming on two wheat cultivars (Fakhar-e-Bhakkar and Akber-19) cultivated under severe drought conditions (35% FC). There were 6 levels of melatonin (i.e., M0 = control, M1 = 1 mg L− 1, M2 = 2 mg L− 1, M3 = 3 mg L− 1, M4 = 4 mg L− 1 and M5 = mg L− 1) which were used for seed priming. Our results confirmed that seed priming with M2 = 2 mgL− 1 concentration of MT alleviates the negative effects of DS by boosting the germination rate by 54.84% in Akber-19 and 33.33% in Fakhar-e-Bhakkar. Similarly, leaf-relative water contents were enhanced by 22.38% and 13.28% in Akber-19 and Fakhar-e-Bhakkar, respectively. Melatonin pre-treatment with 2 mgL− 1 significantly enhanced fresh and dry biomass of shoot and root, leaf area, photosynthetic pigments, osmoprotectants accumulation [total soluble proteins (TSP), total free amino acids (TFAA), proline, soluble sugars, glycine betaine (GB)] and lowered the amount of malondialdehyde (MDA) and hydrogen peroxide (H2O2) production by elevating antioxidants [Ascorbic acid, catalase (CAT), Phenolics, peroxidase (POD) and superoxide dismutase (SOD)] activity under drought stress (DS). Meanwhile, under control conditions (NoDS), the melatonin treatment M1 = 1 mgL− 1 effectively enhanced all the growth-related physio-biochemical attributes in both wheat cultivars. In the future, more investigations are suggested on different crops under variable agroclimatic conditions to declare 2 mgL− 1 melatonin as an efficacious amendment to alleviate drought stress.

Seasickness and partial peripheral vision obstruction with versus without an artificial horizon

Motion sickness (MS) is common when subjects are exposed to unfamiliar motion and affect individuals during travel. This study examines the immediate effect of two visual devices, in the form of glasses, on MS symptoms and associated physiological effects. The hypothesis is that peripheral vision obstruction reduces MS and that an additional beneficial effect could be observed when it is combined with an artificial horizon. Fifteen subjects with moderate to severe susceptibility to MS were exposed to a boat simulator in three conditions. Symptoms were assessed immediately after exposure. Time spent in the simulator, heart rate, and temperature were also recorded. The intensity of symptoms at the end of the experience did not differ, but time spent in the simulator before the onset of symptoms was significantly longer with peripheral vision obstruction (+36 %) and with both techniques combined (+40 %) than in the control condition. No difference was observed between the combined condition and peripheral vision obstruction alone. The glasses device used in this study (with or without an artificial horizon) delays the onset of symptoms. Further research is needed to confirm the mechanism that explains the benefits and to evaluate these effects during prolonged exposure to MS-inducing stimuli or after a period of familiarization with the device.

Electrophysiological and hemodynamic mechanisms underlying load modulations in visuospatial working memory: A functional near-infrared spectroscopy (fNIRS) and electroencephalogram (EEG) study.

The n-back task has been widely used to study working memory. Previous studies investigating the electrophysiological (electroencephalogram [EEG]) and hemodynamic correlates (functional near-infrared spectroscopy [fNIRS]) of the n-back task have been generally based on verbal stimuli and only investigated EEG frequency bands. We simultaneously acquired the EEG and fNIRS in 35 participants (16 males; age = 26.4 ± 4.3 years; educational attainment = 18 ± 2 years) during a visuospatial n-back task. The task encompassed a control condition and a low (requiring to recall one previous stimulus) and a high (requiring to recall two previous stimuli) working memory load experimental conditions. Accuracy decreased and reaction times slowed in the high compared to both low load and control conditions. Regarding EEG, P3a showed higher amplitude in the experimental conditions compared to the control one, and P3b exhibited higher amplitude in the low compared to the high load condition. Regarding fNIRS, the high load condition showed higher deoxygenated hemoglobin compared to the control one. Moreover, the central frontopolar cortex showed higher activation compared with the left frontal cortex. Our study showed that working memory load during a visuospatial n-back task influenced behavioral and electrophysiological indices. Even if the load effect was only observed for deoxygenated hemoglobin on hemodynamic data, this was in line with previous studies and coherent with its electrophysiological correlates. Thus, our study confirms that EEG and fNIRS can be successfully used in multimodal acquisitions, but also highlights that future studies are needed to develop a novel version of the task. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Effects of excess sodium consumption on arterial function in C57BL/6 mice.

Excess sodium consumption contributes to arterial dysfunction in humans. The C57BL/6 strain of mice has been used to identify mechanisms by which arterial dysfunction occurs after excess sodium consumption. However, there are concerns that C57BL/6 mice have strain-specific resistance to high-sodium (HS) diet-induced hypertension. To address this concern, we performed a meta-analysis to determine if excess sodium consumption in C57BL/6 mice induces arterial dysfunction. Databases were searched for HS versus standard diet studies that measured arterial function [i.e., systolic blood pressure (BP), endothelium-dependent dilation (EDD), and central arterial stiffness] in C57BL/6 mice. A total of 39 studies were included, demonstrating that the HS condition resulted in higher systolic BP than control mice with a mean difference of 9.8 mmHg (95% confidence interval [CI] = [5.6, 14], P < 0.001). Subgroup analysis indicated that the systolic BP was higher in HS compared with the control condition when measured during night compared with daytime with telemetry (P < 0.001). We also identified that the difference in systolic BP between HS and control was ∼2.5-fold higher when administered through drinking water than through food (P < 0.001). A total of 12 studies were included, demonstrating that the HS condition resulted in lower EDD than control with a weighted mean difference of -12.0% (95% CI = [-20.0, -4.1], P = 0.003). It should be noted that there was considerable variability across studies with more than half of the studies showing no effect of the HS condition on systolic BP or EDD. In summary, excess sodium consumption elevates systolic BP and impairs EDD in C57BL/6 mice.NEW & NOTEWORTHY C57BL/6 mice are perceived as resistant to high-sodium diet-induced arterial dysfunction. This meta-analysis demonstrates that excess sodium consumption elevates blood pressure and impairs endothelium-dependent dilation in C57BL/6 mice. Nighttime measurements show more pronounced blood pressure elevation. In addition, sodium administration via drinking water, compared with food, induces a greater blood pressure elevation. These findings may be influenced by outlier studies, as the majority of studies showed no adverse effect of excess sodium consumption on arterial function.

Action Planning Renders Objects in Working Memory More Attentionally Salient.

A rapidly growing body of work suggests that visual working memory (VWM) is fundamentally action oriented. Consistent with this, we recently showed that attention is more strongly biased by VWM representations of objects when we plan to act on those objects in the future. Using EEG and eye tracking, here, we investigated neurophysiological correlates of the interactions between VWM and action. Participants (n = 36) memorized a shape for a subsequent VWM test. At test, a probe was presented along with a secondary object. In the action condition, participants gripped the actual probe if it matched the memorized shape, whereas in the control condition, they gripped the secondary object. Crucially, during the VWM delay, participants engaged in a visual selection task, in which they located a target as fast as possible. The memorized shape could either encircle the target (congruent trials) or a distractor (incongruent trials). Replicating previous findings, we found that eye gaze was biased toward the VWM-matching shape and, importantly, more so when the shape was directly associated with an action plan. Moreover, the ERP results revealed that during the selection task, future action-relevant VWM-matching shapes elicited (1) a stronger Ppc (posterior positivity contralateral), signaling greater attentional saliency; (2) an earlier PD (distractor positivity) component, suggesting faster suppression; (3) a larger inverse (i.e., positive) sustained posterior contralateral negativity in incongruent trials, consistent with stronger suppression of action-associated distractors; and (4) an enhanced response-locked positivity over left motor regions, possibly indicating enhanced inhibition of the response associated with the memorized item during the interim task. Overall, these results suggest that action planning renders objects in VWM more attentionally salient, supporting the notion of selection-for-action in working memory.

High‐frequency (470 kHz) ultrasonics‐assisted room temperature CO2 stripping and fate of Sono exposed solvent

AbstractBACKGROUNDThe conventional CO2 stripping process in solvent‐based postcombustion CO2 capture (PCCC) process uses heating to strip the CO2 (~120 °C). However, the challenges associated with this method are high energy consumption in degassing the CO2 from solvent, solvent loss and degradation resulting from the high –temperatures, resulting in high energy consumption typical of solvent‐based PCCC. The present study demonstrates the use of bath‐type sonication (470 kHz frequency) to remove CO2 from CO2 loaded 30 wt% Monoethanolamine under controlled temperature conditions. Solvent performance was evaluated following exposure to 2 h conventional heating and 75 h sonication.RESULTSIn a batch sono‐assisted process, CO2 stripping became possible at 17.5 °C compared to 102.2 °C using the conventional method. Increasing the sonication time led decreased carbon loading and increased stripping efficiency. The stripping rate was high at the initial stages of treatment. Evaluation of sono‐exposed solvents exhibited decreased pH during CO2 loading and decreased absorption capacity of the conventionally heated sample.CONCLUSIONThe sono‐assisted method consumes 3.57‐foldless energy than conventional heating. Its CO2 stripping rate was found to be higher within 5 min of sonication. Notably, the maximum temperature reached for the 1 h intervening mode of sonication at 470 kHz was 49.49 °C. The reduction in absorption capacity per hour of conventional heating was 24.5%, whereas for sonication it was &lt;0.4% and solvent loss was 19.7% lower than conventional. There was no significant change in the color, pH and density of the sample. A 20.4% higher surface tension than that of the virgin sample was observed. © 2024 Society of Chemical Industry (SCI).

Structural and functional characterization of haemoglobin genes in Labeo catla: Insights into hypoxic adaptation and survival

Haemoglobin (HB) protein comprises four subunits: two identical α-subunits (HBA) and two identical β-subunits (HBB), encoded by the HBA and HBB genes. In this investigation, 5′/3′ RACE PCR (Rapid Amplification of cDNA Ends) was used to obtain complete coding sequences (CDSs) of both the genes from farmed Labeo catla. The resulting CDSs were 432 base pairs and 447 base pairs for HBA and HBB, respectively, corresponding to 143 and 148 amino acids. Phylogenetic analysis revealed close relationships with other cyprinids, with Labeo rohita being the closest relative. Functional analysis and protein structure prediction were conducted using bioinformatics tools. Expression profiling of both genes was checked in various tissues under control (C) and hypoxic (H) conditions. Notably, under hypoxia, HBA and HBB genes were significantly upregulated (P < 0.05) initially, followed by a return to normal expression levels. Similar trends were observed for Hif1α (Hypoxia-inducible factor one alpha) and EPO (Erythropoietin) genes. Additionally, haematological indices also significantly increased corresponding to the gene expressions. However, with the decrease in the expression of these genes an onset of mortality was observed in the hypoxia (H) treated groups. The results of the current study explored the role of haemoglobin genes in adaptation to the hypoxic condition.

Effects of 12 weeks of resistance exercise on 24-hour subjective appetite measured under highly controlled conditions inside a metabolic chamber in older men

Effects of 12 weeks of resistance exercise on 24-hour subjective appetite measured under highly controlled conditions inside a metabolic chamber in older men

Effects on alcohol and substance use of a school-based training intervention for adolescents with ADHD

IntroductionAdolescents with attention-deficit/hyperactivity disorder (ADHD) may be at risk for early, escalating patterns of alcohol and substance use via academic, peer, and familial impairment. Existing school-based interventions for youth with ADHD effectively target these risk factors, yet their effects on alcohol and substance use have not been explored. We examined the immediate and long-term alcohol and substance use outcomes of an evidence-based school-based intervention for adolescents with ADHD. MethodA total of 186 (Mage = 15, 79 % boys, 78 % White, 11 % Hispanic) adolescents with ADHD were randomized to either a school-based training intervention targeting academic and social skills or a treatment-as-usual control group. A subset of youth was followed into emerging adulthood (5 year follow-up; n = 73). Participants reported on their alcohol and substance use behaviors and problems at post-treatment, 6-month follow-up, and 5-year follow-up. ResultsTwo-part hurdle models controlling for prior use and demographics indicated treatment was associated with improvements in substance use outcomes among youth using any substances at 6-month follow-up (β = −0.45). However, among youth reporting any alcohol use at the 5-year follow-up, treatment was associated with worse alcohol use problems relative to the control condition (β = 0.27). Approximately 22 % of intervention participants met criteria for risky drinking behavior compared to 5 % of participants in the control group. ConclusionWe found mixed evidence that a school-based intervention associated with positive outcomes on academic, social, and emotional functioning for adolescents with ADHD also prevented adverse alcohol and substance use outcomes. These unexpected results serve as a call for extended follow-up periods to identify the durability of intervention benefits and potential for downstream iatrogenic effects. Additional research is needed to identify school-based intervention strategies that can effectively deter substance use risk among select populations.

Benefits of a group-based running session on feelings of energy and fatigue: no augmenting effect of green exercise during the defoliation season.

This randomized controlled trial investigated the impact of a brief session of group-based running on feelings of energy and fatigue, comparing exercise in a local nature reserve versus an outdoor built environment, and a sedentary control condition. Sixty-six physically active university students participated, with 56 providing full data for each time point. Consistent with previous research, participants in both exercise groups reported significantly increased energy and decreased fatigue compared to those in the sedentary control group. However, the type of outdoor environment did not significantly influence the magnitude of improvements. Contrary to expectations, there was no significant difference in energy enhancement or fatigue mitigation between participants who jogged in a local nature reserve versus those who jogged on a cinder track in an urbanized area. The study discusses potential reasons for this, including seasonal variations in forest volatile organic compounds (FVOCs) concentrations, which may impact the psychological effects associated with forest environments. Further research is needed to better understand the complex relationship between exercise, environment, and feelings of energy/feelings of fatigue.