Total Aflatoxin Content Research Articles

First evidence on the occurrence of multi-mycotoxins and dietary risk exposure to AFB1 along the cassava value chain in Uganda

This study investigated the occurrence and distribution of multiple mycotoxins (aflatoxin B1, B2, G1, G2, fumonisins B1, B2, ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN), and citrinin (CIT)) in cassava products and as assessed the potential risk of aflatoxin B1 (AFB1) exposure among cassava consumers. A total of 192 samples of cassava products (96 flour and 96 chips, each with 48 samples from farmer and 48 from wholesaler) were analysed using LC/MS–MS. All positive samples irrespective of their origin (flour or chips) exhibited AFB1 levels exceeding the EU regulatory threshold of 5 µg/kg. The sum of fumonisins (FB1 + FB2), ZEN, and DON were significantly (P < 0.05) higher in cassava flour (14.3 µg/kg; 3.71 µg/kg; 25.1 µg/kg) compared to chips (6.54 µg/kg; 1.25 µg/kg; 0.25 µg/kg), respectively. Aflatoxins G2 was not detected in any of 192 samples. Cassava flour samples from farmers exhibited significantly (P < 0.05) higher mean concentrations of AFB1 (27.1 µg/kg), total aflatoxins (78.2 µg/kg), and ochratoxin A (79.6 µg/kg) in contrast to wholesalers, whose mean levels were notably lower at 8.91, 5.79 µg/kg, and 2.44 µg/kg, respectively, pointing the likely critical source of mycotoxin contamination. Cassava consumers in Northern Uganda are at a higher risk, with an estimated 2.06 cancer cases per 100,000 individuals per year compared to those in Eastern Uganda at 0.25. This study underscores the urgent need for interventions to manage aflatoxins in cassava flour, particularly at farm level in Northern Uganda. It accentuates a shift market to household-level sampling and the need for analytical methods targeting multiple mycotoxins.

A study on the performance of corn grain dryer using hybrid solar drying with liquefied petroleum gas

Corn is a widely produced and consumed agricultural product, used as fuel, staple food, and livestock feed. The high level of corn production can lead to potential post-harvest food losses. Drying methods offer a solution to extend the shelf life of a substance. Hybrid solar drying, which combines solar drying with additional heating, particularly using liquefied petroleum gas (LPG), was introduced in this research to ensure faster and more efficient drying processes. The three drying methods compared in this study consist of open sun drying, natural solar drying, and hybrid solar drying. The research results show an average corn grain moisture content of 13.95% over 10.5 hrs, 13.75% over 6 hrs, 13.65% over 7 hrs, 13.45% over 5 hrs, and 13.65% over 4.5 hrs in open sun drying, natural solar drying, and hybrid solar drying with temperature variables of 40°C, 50°C, and 60°C. The average drying rates were 0.212 g/min, 0.364 g/min, 0.318 g/min, 0.439 g/ min, and 0.477 g/min, respectively. The average drying efficiency values for each variable were 2.968%, 4.719%, 5.358%, 5.271%, and 6.037%. In the proximate test, the corn grain moisture content in the hybrid solar drying variable at 60°C was 13.8%, ash was 1.24%, protein was 7.76%, fat was 4.40%, and carbohydrate was 72.8%. For the corn grain color test, the average values obtained were L = 46.48, a = 4.58, and b = 25.37. Meanwhile, the aflatoxin test for dried corn grains showed values of aflatoxin B1, B2, G1, and G2 &lt;0.5 with a total aflatoxin content &lt;2.00. Therefore, hybrid solar drying is recommended over open sun drying and natural solar drying because of its ability to operate even in unfavorable weather conditions, its temperature control capability, its ability to accelerate the drying process, and its capacity to maintain the quality of the material.

Climatic effects on aflatoxin contamination of maize

Aflatoxins are frequent contaminants of maize especially in the face of climate change with deleterious health and socio-economic impacts. South Africa is ranked 9th maize exporter globally; hence, insights need to be gained in terms of the maize value chain in South Africa with respect to aflatoxin contamination to evaluate consumers’ exposure. High-performance liquid chromatography (HPLC) technique was used in this study to quantify aflatoxins in South African commercial maize. One thousand and twenty-eight (1028) maize samples were collected across six distinct agro-climatic regions over five harvest seasons (2017 – 2021). A total of 205 samples (19.94 %) were found to be contaminated with aflatoxins, with mean total aflatoxin concentration of 64.17 ppb amongst the contaminated samples, which is above the SA regulatory limit of 20 ppb for animal consumption. The year 2018 recorded the highest mean total aflatoxin value while North-West agro-climatic region had the highest mean total aflatoxin value. Drastic reduction in average rainfall significantly influence aflatoxin contamination of South African maize.

The occurrence and human health risk assessment of total and aflatoxin B1 in selected food commodities in Bhutan

Aflatoxins are mycotoxins that contaminate staple foods globally and pose a significant health risk. To the best of our knowledge, information on the occurrence of aflatoxins in Bhutanese diets is scarce. This study aimed to estimate the aflatoxin levels in selected foodstuffs in Bhutan and determine the health risk associated with aflatoxin exposure. Ten different types of food commodities were randomly collected from farmers’ markets, shelves of supermarkets, and wholesale and retail shops from 20 districts of the country. The samples were subjected to analysis by an enzyme-linked immunosorbent assay for both total aflatoxins (B1, B2, G1 and G2) and aflatoxin B1. Among the 315 samples included, 48.81% and 79.35% were positive for total aflatoxins and aflatoxin B1, respectively. The overall mean total aflatoxin concentration was 11.49 ± 12.83 µg/kg, and that for B1 was 17.62 ± 23.99 µg/kg. The most prevalent food commodity with the highest aflatoxin contamination was chili products. In addition, the estimated daily intake and margin of exposure to aflatoxin B1 via the consumption of chili products ranged from 0.98 to 5.34 ng kg−1 bw day−1 and from 74.90 to 408.10, indicating a risk for public health. The liver cancer risk was estimated to be 0.01 and 0.007 cancers per year per 100,000 population resulting from the consumption of chili products. The present findings revealed the presence of total aflatoxins and aflatoxin B1 in the selected samples. The margin of exposure values was exorbitant, demanding a stringent public health measure. Notably, these results suggest the need for routine monitoring of aflatoxin contamination in the region and stress rigorous safety management strategies to reduce exposure.

AFLATOXIN CONTAMINATION AND NUTRITIONAL EVALUATION OF DRIED JUVENILE FISH (Clarias gariepinus) SOLD IN IBADAN METROPOLITAN MARKETS

The contamination of food due to the occurrence of mycotoxigenic fungi is a public health concern. Therefore, fungi and aflatoxin contamination of smoked-dried juvenile fish (SDJF) (Clarias gariepinus) were investigated. The samples were purchased from three major markets in Ibadan. The total fungal count ranged from 1.0 x 103 to 12.0 x 103 CFU/g. Thirty-two fungi were isolated, including Penicillium sp., Rhizopus sp., Alternaria sp., Aspergillus flavus, Aspergillus niger, and Aspergillus ochraceus. A. niger had the highest frequency of occurrence (38%), while A. ochraceus (3%) had the lowest. All the samples were contaminated with aflatoxin. The total aflatoxin content ranged from 0.237 to 1.995 ppb. Of the 11 Aspergillus strains obtained from the samples, 5 were aflatoxigenic, while 6 were not. 85 and 94% of the mycobiota were xerophilic and halophilic. The percentage moisture content, crude protein, crude fat, and ash content in the samples ranged from 12.99–17.82%, 68.72–77.62%, 0.49–6.00%, and 6.97–12.24%. Phosphorus and potassium concentrations of the SDJF samples ranged from 388.2–509.6 mg/100g and 1113.8–1517.0 mg/100g, while lead and cadmium were not detected in the samples. Though the level of aflatoxin contamination in this study was within the maximum limit permitted (20 ppb), proper attention is needed for adequate preservation before sales and consumption.

Influence of climatic factors on spatio-temporal variabilities in the occurrence of aflatoxins in major food grains available at retail shops in Bangladesh

In Bangladesh, little is known about the role of climatic factors influencing spatio-temporal occurrence among different food grains of aflatoxins, which have been linked to liver cancer cases and other health hazards. In this study, 252 food grain samples of 10 types were collected from retail markets at 18 districts, pre-monsoon, monsoon and post-monsoon seasons during February-September, 2018. Occurrence and concentration of total aflatoxins in the samples were analysed by a direct competitive enzyme-linked immunosorbent assay, and compared with climatic data (temperature, humidity, rainfall, and sunshine hours). Contamination of aflatoxins was found in approx. 38% (n=96) of the total samples, with highest occurrence in groundnuts (82%), followed by corn grains (70%), chick pea (54.5%), wheat grain (50%) and green gram (42.9%). Aflatoxins occurrence was moderate in split chick pea (30.8%), yellow pea (28.2%), black gram (27.8%), and lentils (27.6%), while the lowest in grass pea (17.6%). Aflatoxins concentration was found to exceed the European Union permissible limit (≤5 µg kg-1) in 33 (13.1%) samples. Overall, approximately 5.6%, 7.5%, and 25.0% samples were contaminated with high (10-19 ppb), medium (5-9 ppb) and low (1-4 ppb) concentrations of aflatoxins, respectively. High level concentration of aflatoxins was more frequent in groundnut (36%) and wheat grain (20%), in comparison to other types. Occurrence of aflatoxins during pre-monsoon was detected among 21% (19/91) samples, which increased to ca. 30% (36/119) during monsoon, and 98% (41/42) during post-monsoon. However, aflatoxins mean concentration during post-monsoon was comparatively low than the other seasons. Variations in aflatoxins monthly prevalence correlated significantly with relative humidity (p ≤ 0.01) and rainfall (p≤ 0.05), when a 1-month time lag was considered. Therefore, predisposing climatic conditions, i.e., rainfall incidences and persistence of higher relative humidity in the previous month(s), have salient influence on aflatoxins occurrence, potentially impacting both pre-harvest and stored food grains. This study underscores the need of a more holistic monitoring of aflatoxins in agricultural products for a longer term, and adoption of proper intervention measures for food grains while being stored and before consumption. Asian Australas. J. Food Saf. Secur. 2024, 8(2), 32-47

Mycological detection of aflatoxin producing gene (Aflr) in Aspergillus Spp found in commercial raw groundnut and groundnut cake (Kuli kuli) in some selected market in Katsina state, Nigeria

The effect of aflatoxin intoxication from groundnut and groundnut products has lead to a large amount of deaths some of which are not yet accounted of. Kuli-kuli formally referred to as groundnut cake, is a byproduct of oil extraction from groundnut which have been further fried, sweetened or spiced. This research aimed at determining the mycological quality and molecular detection of Aflatoxin gene aflR in Aspergillus spp found in raw Groundnut and groundnut cake (Kuli-kuli). A total of 120 samples (60 groundnut samples and 60 groundnut cake samples) were randomly collected from four selected markets in Katsina state and subjected to mycological assessment based on colonial morphology on PDA and microscopy. Out of the total samples, 16 samples (8 raw groundnut samples and 8 groundnut samples) were selected for determination of total Aflatoxin concentration using ELISA technique. Isolates were also screened for aflR gene using polymerase chain reaction technique, and subsequently sequenced using Sanger method. Eleven (11) fungi isolate including Aspergillus niger, Scedosporium apiospermum, Cladophialophora bantiana, Aspergillus flavus, Aspergillus nidulans, Microsphaeropsis arundinis, Cladosporium, Cladophialophora carrionii, Rhizopus arrhizus, Aspergillus parasitus and Aspergillus fumigatus were identified to produce aflatoxin concentration ranging from 0.4–3.5 ppb from the samples of the raw groundnut and groundnut cake (Kuli-kuli). Therefore, the findings of this research has raised serious food safety concerns, especially in consumption of raw groundnut and groundnut cake (Kuli-kuli) sold in Katsina as such relevant regulatory agencies such as NAFDAC are advised to deploy drastic measure to curtail a possible aflatoxin intoxication outbreak

Standardization of Phomopsis sp. extract and prediction of anti breast cancer activity from the main compound through molecular docking

Abstract Endophytic fungi isolated from Indonesian soursop (Annona muricata) leaves, which have the potential to treat breast cancer, have been successfully isolated since 2016. Identification based on the Internal Transcribed Spacer (ITS) gene revealed that fungi close to Phomopsis sp. The fungi were stored, subcultured, and extracted with ethyl acetate for in vitro and in vivo studies. To be registered as a medicinal ingredient and meet the requirements of the Indonesian Food and Drug Supervisory Agency (BPOM, Badan Pengawas Obat dan Makanan), it is necessary to ensure the standardization, safety, and quality of the Phomopsis extract. The results showed that the Phomopsis extract met the BPOM requirements for organoleptic, water, and solvent content, microbial and heavy metal contamination, total aflatoxin, and metabolite content. Analysis of the Phomopsis extract content also showed that the compounds in the two production batches were stable, with the highest relative abundance of 7-hydroxycoumarin. In silico studies with molecular docking show that the affinity energy of 7-hydroxycoumarin docking with the estrogen receptor alpha (ERα) and thymidine kinase 1 (TK1) has a lower negativity value than the comparison ligand (-6.7 and -6.8 kcal/mol).

Evaluation of Aflatoxins and Heavy Metals Exposure in the Local Feeds of Fish and Shrimp in Bangladesh.

This study aims to comprehensively assess the presence of aflatoxins, pervasive fungal toxins, and heavy metals (lead, chromium, cadmium) in the local feed of fish and shrimp. Forty-eight samples of the fish and shrimp feed were collected from the Dhaka and Sathkhira districts of Bangladesh respectively. Aflatoxins (B1, B2, G1, G2) were analyzed by high-performance liquid chromatography (HPLC) with fluorescence detector, and atomic absorption spectrophotometry (AAS) was used for the heavy metal detection after sample digestion. The standard analytical methods of the Association of Official Analytical Chemists were followed for analyzing the proximate composition of the feed samples. The findings revealed the high toxic concentration of total aflatoxins particularly in fish feed (196.25 μg/kg). Concurrently, varying concentrations of heavy metals, including lead (Pb) and chromium (Cr), were detected, with the highest levels found in shrimp feed at 3.324 and 174.6 mg/kg, respectively. In comparison, highest contamination of cadmium (Cd) was found in fish feed at 0.398 mg/kg. The outcomes are growing concerns regarding the quality of locally sourced feed and emphasizing the need for prompt corrective actions. Otherwise, neglecting this concern may lead to the possibility of both fish and, ultimately, humans who consume them being vulnerable to the absorption and accumulation of these assessed toxic compounds.

The Possible Role of Mycotoxins in the Pathogenesis of Endometrial Cancer.

Endometrial cancer is one of the most common cancer types among women. Many factors can contribute to the development of this disease, including environmental factors and, thus, eating habits. Our study aims to determine the levels of various mycotoxins and their metabolites in the blood serum and endometrial tissue samples of participants with previously proven endometrial cancer and to find possible contributions to cancer development. In the cohort clinical trial, 52 participants aged between 44 and 86 were studied. The participants were divided into two groups: patients or matched controls. All patients had previously histologically diagnosed endometrial cancer. The cancer patients were divided into low-grade endometrioid and low- plus high-grade endometrioid groups. Controls had no history of endometrial malignancy or premalignancy. Blood serum and endometrial tissue samples were obtained from all study patients. We compared the concentrations of total Aflatoxins (Afs), Deoxynivalenol (DON), Ochratoxin-A (OTA), T2-toxin and HT2 toxin (T2/HT2 toxin), Zearalenone (ZEN), alpha-Zearalenol (α-ZOL), and Fumonisin B1 (FB1) in the serum and endometrium between the different study groups. As a result, we can see a significant correlation between the higher levels of Afs and zearalenone and the presence of endometrial cancer. In the case of Afs, DON, OTA, T2/HT2 toxins, ZEN, and alpha-ZOL, we measured higher endometrial concentrations than in serum. Considering the effect of mycotoxins and eating habits on cancer development, our results might lead to further research exploring the relationship between certain mycotoxins and endometrium cancer.

Sustainable Processes and Physico-Chemical Characterization of Artisanal Spontaneous Gluten Free Sourdough (Quinoa, Amaranth and Brown Rice) Compared to Wheat Sourdough

The industry predominantly depends on synthetic or artificial additives, occasionally permitting the inclusion of natural molecules sourced from plants or replicated from their original counterparts. The production of bakery products increasingly uses sourdough to improve the quality of bread or to obtain “clean label” products (free of artificial additives). The additive production sector contributes to this concern through the synthesis of potentially harmful compounds, the utilization of hazardous chemicals and solvents, the management of resulting by-products, and reliance on non-renewable resources for manufacturing. One percent of the world’s population suffers from celiac disease. Celiac disease is treated by excluding gluten from the diet. Most gluten-free bakery products have low nutritional and sensory quality. Therefore, sourdough is being used to replace chemical yeast to improve the sensory and nutritional quality and increase the shelf life of gluten-free bakery products. Three gluten-free sourdoughs were prepared with different flours: brown rice, quinoa and amaranth, in order to compare them with traditional sourdough (wheat) and optimize the most suitable temperature for the conservation of sourdoughs. Physicochemical analysis (pH, titratable acidity and color), antioxidant activity (FRAP, ORAC and ABTS), total phenolic compound content (Folin–Ciocalteu), total aflatoxin content, lactic and acetic acid content and microbiological analysis (mold and yeast content and bacterial and fungal composition (microbiota composition)) were carried out during the elaboration process and at different storage temperatures. A higher microbiological quantity of molds and yeasts (7.97 log CFU/mL), non-Saccharomyces yeasts (7.78 log CFU/mL) and lactic acid bacteria (8.10 log CFU/mL) and fungal composition were observed in the amaranth sourdough. The wheat sourdough obtained a higher total content of phenolic compounds (33.03 mg GAE g−1) and antioxidant capacity in ABTS and FRAP, but the quinoa sourdough had the highest ORAC content. In addition, it was observed that the adequate temperature for the conservation of the doughs is 25 °C, due to the predominance of Lactobacillus spp. and Pediococcus spp. bacteria in the sourdough. Therefore, pseudocereal sourdoughs (quinoa and amaranth) could be an alternative to incorporate into the preparation of gluten-free bread, since their microbial composition, physicochemical composition, antioxidant activity and total phenolic compounds would contribute to gluten-free bread and thus produce health benefits for people with celiac disease.

Evaluation of rapid alert system for food and feed (RASFF) data for mycotoxin contaminated dried figs originating from Türkiye

In this study, the Rapid Alert System for Food and Feed (RASFF) database was analysed to assess mycotoxin hazards in Turkish dried figs during the production years from 2002 to 2019. A comprehensive evaluation of influencing factors, such as weather conditions, agricultural and processing practices, as well as non-product related elements such as export quantities and regulatory changes, was carried out while analysing yearly and monthly notification trends. Years marked by drought conditions recorded the highest number of notifications. Notably, the years 2007 and 2012 corresponded with peaks in notifications (104 and 117, respectively), suggesting a potential relationship between drought conditions and increased notifications – a finding supported by existing literature on mycotoxin contamination. The mean total aflatoxin (AF) concentrations ranged from 13 μg/kg to 44 μg/kg. However, the considerable variability in the annual number of notifications (ranging from 2 to 106) and the presence of outliers in certain years limit the usefulness of mean total AF concentration data for trend analysis. Weather-related factors, along with agricultural practices, processing and storage conditions, can influence mycotoxin contamination of dried figs, highlighting the need for integrated and comprehensive control strategies. The observed correlation between specific weather conditions, such as drought, and RASFF notifications, and, consequently, mycotoxin contamination, warrants further exploration to better understand and predict this relationship. This study significantly contributes to the mycotoxin literature by providing a comprehensive analysis of mycotoxin hazards in Turkish dried figs. By examining the complex interplay of factors, from weather conditions to agricultural and processing practices and changes in export quantities and regulations, it addresses a crucial gap in the current understanding of mycotoxin risks and offers insights on the historical evolution of safety protocols within Turkish dried fig production. It thus provides a solid foundation for improving food safety practices and developing more effective policy frameworks.

Assessment of Total Aflatoxin Content in Dry Fruits Samples Collected from Local Markets of Lahore, Pakistan

Assessment of Total Aflatoxin Content in Dry Fruits Samples Collected from Local Markets of Lahore, Pakistan

Evaluation of aflatoxin content in “Cheka” (traditional beverage in South-Western Ethiopia) and its major ingredient (maize)

BackgroundAflatoxin is a toxic cancerous fungal secondary metabolite produced by Aspergillus species. Consumption of aflatoxin-contaminated cereals and their products can cause several health-related effects including acute and chronic aflatoxicosis. This study aims to evaluate the total aflatoxin content in “Cheka” and its major ingredients using the Immuno-Affinity Column Clean-up technique by sensitive HPLC (High-Performance Liquid Chromatography) with fluorescence detection.MethodsThis study followed a completely randomized experimental design to evaluate the level of aflatoxin in traditionally brewed “Cheka” and its major ingredients (maize) by HPLC from November 2019 to June 2020. Traditionally brewed 6 “Cheka” beverages and 6 maize seed samples were collected randomly from Konso and transported to Bless Agri Food Laboratory in Addis Ababa for laboratory evaluation of aflatoxin in samples. The data were analyzed by SPSS version 25.ResultsIn the present study aflatoxin contamination was detected in all maize and “Cheka” samples. A mean total aflatoxin level of 6.78 ± 8.28 μg/kg and 10.76 ± 4.50 μg/kg were observed in maize and “Cheka” samples respectively. The maize taken from above-ground storage (“Gotera”) and underground storage (pitfall) showed a mean total aflatoxin level of 10.23 ± 10.12 μg/kg and 3.35 ± 5.79 μg/kg respectively. The samples taken from “Cheka” prepared from maize of above-ground storage (“Gotera”) and underground storage (pitfall) showed a mean total aflatoxin level of 11.34 ± 3.06 μg/kg and 10.19 ± 6.35 μg/kg respectively. Aflatoxin G family was not detected in both maize and “Cheka” samples while aflatoxin B1 and B2 contributed to the total aflatoxin level. AFB1 predominantly contributed to the contamination of all samples. Although there was no significant difference while comparing between mean total aflatoxin of any other sample types, a significant difference was observed for the AFB1 mean level of pitfall maize and their respective “Cheka” samples (P = 0.033).ConclusionThe mean level of AFB1 in pitfall maize differs from a respective “Cheka” sample. As most of the samples evaluated for aflatoxin contamination showed levels above healthy cut-off points of aflatoxin set by different authorities like the European Union, CODEX, and Ethiopian Standards Agency, there must be a protracted interventional approach to reduce aflatoxin contamination in maize and its products in the region.

MOLECULAR TECHNIQUES FOR DETECTION OF AFLATOXIGENIC ASPERGILLUS FLAVUS AND DETERMINE THEIR AFLATOXIN IN PISTACHIOS.

The better approach to control the contamination of food by aflatoxin (AF), is identifying the fungal strains responsible for producing that toxin. To estimate the presence of aflatoxigenic fungi growth and AFs in pistachios, thirty two pistachios samples (8 fresh and 24 salt roasted) were collected from Sulaimani markets (Iraq).Rapid screening method for AFs production were utilized initially based on culture methods, the positive samples confirmed by polymerase chain reaction (PCR) that were applied using DNA extracted from the isolates of A. flavus targeting the genes (afR, aflP, aflD, and aflQ). For determination of AF production of those isolates, ELISA technique was applied. The results show, contamination of 20 (62.5%) samples of pistachios with fungi, among them A. flavus was the predominant. Ten (71.4% (of the A. flavus isolates were amplified the target genes by applying PCR, indicating their ability to produce AFs. Application of ELISA technique revealed AFs detection in 21(65.6%) samples of pistachios. The mean levels of AFs determined were 6.06 and 12.08 μg/kg in fresh and salt roasted samples respectively. 19 (59.4%) of pistachio samples were exceed the maximum allowable limit set by the European union of total AFs concentration .Strong correlation obtained between AFs gene expression with AFs production. However, to avoid false negative and false positive results, applying more than one diagnostic molecular technique were suggested.

Evaluation of the Level of Aflatoxins in Raw Peanuts and Reduction Levels of Aflatoxins in Peanuts through Nixtamalization (Lime Treatment) and Calcium Enrichment

Nixtamalization has been widely used as a method of reducing aflatoxin contamination in peanuts as well as in calcium enrichment. The aim of this study is to investigate the effects of nixtamalization on aflatoxin and nutrient content in peanuts among wholesalers and retailers at Nyamakima Market in Nairobi, Kenya. A representative sample was drawn from a population of 70 market vendors using a systematic random sampling technique, resulting in 35 raw peanut samples for analysis via ELISA to determine initial aflatoxin levels. These samples were then subjected to nixtamalization treatments of 4, 8, and 16 minutes. The findings revealed that the average concentration of total aflatoxins in peanuts initially exceeded the accepted safety limit, with 71% of the samples failing to meet the standards. However, nixtamalization led to a substantial reduction in aflatoxin levels by 49%, 48%, and 50% after treatment durations of 4, 8, and 16 minutes, respectively. Statistical analysis, including ANOVA and Two-sample T-tests, confirmed significant distinctions between initial and final mean aflatoxin levels post-treatment, as well as variations between treatments. The study also observed a significant variation in calcium levels between the different time treatments, supported by linear regression analysis, which demonstrated a robust correlation between time treatments and calcium levels (R2=0.83, P=0.011). Furthermore, the analysis revealed significant differences in mean moisture contents across various treatments (P=0.000), and a linear relationship between mean moisture content and exposure time (P=0.02). The results emphasize the positive impact of nixtamalization in reducing aflatoxin levels and enhancing calcium content in peanuts. However, it is crucial to note the negative effect on moisture content, which exceeded stipulated limits, potentially raising concerns about product safety and quality.

Occurrence of aflatoxigenic fungi and aflatoxins in maize grains and associated awareness and handling practices among farmers and traders in South Sudan

Maize is a popular staple food among the urban population of South Sudan. However, due to inadequate food safety surveillance and enforcement systems, there is limited information on the aflatoxin safety of maize grains at farm and market levels. Therefore, this study aimed at assessing aflatoxins awareness and handling practices of maize grains among randomly selected farmers (n=30) and traders (n=30) as well as determining the moisture content, Aspergillus species counts and aflatoxins contamination. A cross-sectional descriptive study was carried out in Magwi maize producing areas, Juba retail/wholesale markets and Nimule border points of South Sudan. Moisture content, fungal and aflatoxins contamination in maize grain samples were determined using dry air oven method, dilution plating technique, and High-Performance Liquid Chromatography (HPLC), respectively. The respondent results showed that farmers (97%) and traders (83%) were unaware of aflatoxins. About 83% of the farmers dried their maize grains on tarpaulins, for 4 - 5 days (77%). In addition, most farmers (77%) stored their maize grain bags on raised platforms, whereas most traders (73%) stored grains on bare ground. All the maize grains met the required moisture content limit (below 13.5%). Maize from Nimule main park had the highest levels of contamination with Aspergillus flavus (9 log CFU/g), Aspergillus parasiticus (12 log CFU/g), aflatoxin B1 (505.56 µg/kg) and total aflatoxins (1,032.19 µg/kg). Maize from Gudele market was contaminated with Aspergillus parasiticus (12 log CFU/g), and aflatoxin B1 (76.55 µg/kg), and had a total aflatoxin content of 94.09 µg/kg. Omeo farmers’ maize grains had the least levels of contamination of Aspergillus parasiticus (6 log CFU/g), aflatoxin B1 (4.39 µg/kg), and total aflatoxins (7.83 µg/kg). In addition, Aspergillus flavus was not detected from Omeo farmers’ maize grains and no aflatoxins were detected from Agoro and Paluonganyi farmers’ grains. This study recommends wider aflatoxins awareness and regular aflatoxin screening of maize grains by the relevant stakeholders in South Sudan. Key words: aflatoxins, aflatoxigenic fungi, awareness, farmers, handling practices, maize, South Sudan, traders

Exploration of aflatoxigenic potential and seed colonization of Aspergillus flavus in sunflower

Aflatoxin, a mycotoxin found commonly in maize, peanuts, and sunflower worldwide, is associated with liver cancer, acute toxicosis, and growth impairment in humans and animals. In India, sunflower seeds are a source of snacks, cooking oil, and animal feed. These seeds are a potential source of aflatoxin contamination. However, reports on aflatoxin contamination in sunflower seeds and cakes are scarce. The objective of the current study was to determine the potential of Aspergillus flavus and total aflatoxin concentrations in sunflower seeds and cakes from small-scale oil processors in Rohilkhand region of Uttar Pradesh. 126 cultures of Aspergillus flavus were obtained from sunflower kernels, which showed wide variation in their cultural characters. Colour of conidial heads being a stable character was used to divide them into four groups out of which two representative isolates each, based on minimum and maximum number of sclerotia cm-2 of the culture medium were selected. Aflatoxin production by an isolate had positive correlation with the sclerotia production. These results can help in identifying the potent of A. flavus isolates for aflatoxin production and developing proper management strategies. In summary, humans and animals are potentially at high risk of exposure to aflatoxins through sunflower seeds and cakes from micro-scale millers in India and location influences risk.

Incidences of aflatoxin contaminations in ingredients, feed and products of poultry from two regions in Ghana

Commercial poultry feed required for proper growth of birds and egg production contains essential nutrients with maize as key component. Inadequately dried maize is prone to aflatoxin contamination and therefore when used in feed formulation for poultry may compromise the safety of the feeds and poultry products. This study investigated the levels of aflatoxins in feed ingredients, feed and poultry products sampled from Eastern and Greater -Accra regions of Ghana. The aflatoxin levels of B1, B2, G1, and G2 were determined using a High-Performance Liquid Chromatography (HPLC) methodology. Main feed ingredients used were fishmeal, cotton seed cake, soya meal, rice, wheat and maize bran as well as maize grains. There was correlation between the level of aflatoxins and moisture content in poultry feed ingredients. All the poultry feeds (100 %) analysed showed the presence of aflatoxins with total aflatoxins recorded ranging from 5.32 to 29.88 μg/kg. Maize samples of the poultry feeds, from all two regions, revealed maize to be a major contributor to the overall total aflatoxin contents found in the feed. Five (5) out of ten (10) communities investigated in the two (2) regions where the poultry feeds were examined recorded total aflatoxin levels in maize above the Ghana Standard Authority (GSA) specification of 20 μg/kg. Aflatoxins G1 and G2 were not detected in all samples of chicken meat and eggs. Total aflatoxin levels recorded for all chicken meat and eggs were below GSA specification of 5 μg/kg; implying that these products were safe for consumption.

Investigation of total aflatoxin in nuts and dried fruits and consumption habits during pregnancy in Türkiye

Background: During pregnancy, adequate energy, nutrient intake, and food safety are important. Aims: This study aimed to examine the presence of aflatoxin, which has toxigenic properties, in nuts and dried fruits consumed during pregnancy. Subjects and Methods: Forty-five pregnant women living in Istanbul and Balikesir cities provinces, who were selected using the convenience sampling method, were applied face-to-face interview method on their attitudes and behaviors regarding their preferences for consuming nuts and dried fruits and storage conditions between February and April 2022. For aflatoxin analysis, the samples were taken from the nuts and dried fruits consumed by the pregnant women. The total aflatoxin content in the samples was analyzed by the ELISA method with a quantitative aflatoxin high-sensitivity test kit. Results: The most commonly consumed nuts by pregnant women during pregnancy; were walnuts and hazelnuts, respectively. It was determined that pregnant women buy nuts and dried fruits first from the nut shop and second from the market. Regarding storage preferences, it was seen that the participants mostly stored the nuts in the closed kitchen cabinet or refrigerator cabinet. For the storage material preferences, glass, porcelain, and packaging were preferred. For aflatoxin results, no detectable level of aflatoxin was found in 35 samples, and the presence of aflatoxin in 3 samples (1.43 ppb, 1.523 ppb, and 1.804 ppb, respectively) was detected. Conclusion: Nuts and dried fruit consumption preferences of pregnant women differed according to the products. No aflatoxin was found in the nuts and dried fruits at a level that could threaten the health of pregnant women. Keywords: Aflatoxin, Nuts, Dried Fruits, Pregnancy, ELISA method