Concentrations Of Tannery Effluent Research Articles

Chromium phytoaccumulation and its impact on growth and photosynthetic pigments of Spirodela polyrrhiza (L.) Schleid. on exposure to tannery effluent

Spirodela polyrrhiza (L.) Schleid. (giant duckweed)—a widespread aquatic macrophyte was found to be a potential chromium bioaccumulator in the present study. To assess the tolerance and hyperaccumulation of chromium by S. polyrrhiza, the plants were exposed to 25, 50, 75, and 100% concentrations of tannery effluent under laboratory conditions for 7 days. Significant toxic effects on the S. polyrrhiza plant were observed, as revealed by reduction in growth parameters as well as photosynthetic pigments in comparison to the control. Despite exhibiting severe phytotoxicity symptoms, the roots and fronds of S. polyrrhiza accumulated the highest amount of chromium on exposure to 100% tannery effluent (roots: 64,841.8 mg g−1; fronds: 10,478.4 mg g−1) after 7 days of exposure. Our results point to S. polyrrhiza as a proficient species to be used in the exploration of chromium hyperaccumulation as well as a prospective contender for tannery wastewater remediation.

Histological liver chances in Swiss mice caused by tannery effluent.

Although tannery effluents are known for being highly toxic to organisms, reports about the effects of the intake of these xenobiotics on experimental mammal models are recent. Studies about the damages the chronic intake of these effluents can cause in the liver of outbred mice remain an unexplored field. Thus, the aim of the present study is to assess (histological) the hepatic condition of Swiss mice (outbred strain) chronically exposed to the intake of different raw tannery effluent concentrations diluted in water for 150days. Accordingly, the mice (males and females) were divided in the following groups: control group-animals treated with drinking water, only; and groups 5 and 10%-treated with raw tannery effluent diluted in water. After exposure, the animals were subjected to euthanasia for liver fragment sample collection and histological analysis, respectively. Moderate hydropic degeneration was observed in the centrilobular regions of the liver of mice exposed to 5 and 10% tannery effluent, as well as greater amounts of hepatocytes presenting karyomegaly and necrotic hepatocytes, and a smaller amount of Kuffer cells in the liver of mice exposed to the xenobiotic. Finally, animals exposed to 10% tannery effluent showed mild hyperplasia of the bile ducts in the portal areas and fibroblast proliferation around the bile ducts, thus suggesting a fibrous process. Except for the frequency of hepatocytes presenting karyomegaly (lower in females), the herein observed hepatic changes were similar in male and female Swiss mice. Accordingly, the present data support the hypothesis that the chronic intake of tannery effluent by outbred mice (Swiss) causes damages in the liver, a fact that broadens the knowledge about the toxic potential of this pollutant, which goes beyond that of C57Bl/6J male mice (inbred strain).

Aneugenicity and clastogenicity in freshwater fish Oreochromis niloticus exposed to incipient safe concentration of tannery effluent

Conventional effluent bioassays mostly rely on overt responses or endpoints such as apical and Darwinian fitness. Beyond the empirical observation, laboratory toxicity testing needs to rely on effective detection of prognostic biomarkers such as genotoxicity. Indeed, characterization of tannery effluent requires slotting in of genotoxic responses in whole effluent toxicity testing procedures. Hence, the prime objective of the present experimental investigation is to apply the technique of biological assay as a tool of toxicity testing to evaluate the induction of micronuclei (MN) in peripheral erythrocytes, and exfoliated cells of gill and kidney of O.niloticus exposed to Maximum tolerable concentrations (MTCs) of composite Modjo tannery effluent (CMTE) and to compare the sensitivity of each cells origin to the induction of MN. After 72h of exposure, cellular aberrations were detected using MN and nuclear abnormality (NA) tests. The induction of MN was significantly higher in exposed groups (P<0.05) when compared to the control group; moreover the tissue specific MN response was in the order, gill cells>peripheral erythrocyte>kidney. Total NA was found to increase significantly (P<0.05), when compared to the non-exposed group. NA was also further ramified as blebbed (BL), bi-nucleated (BN), lobbed (LB) and notched (NT) abnormalities. The result of each endpoint measured has demonstrated that at a concentration of total chromium (0.1, 0.73 and 1.27mg/L), a perceptible amount cellular aberration was measured, further implicating somber treat of genotoxicity to fishes, if exposed to water contaminated with tannery effluent. This further highlight that conventional effluent monitoring alone cannot reveal the effects expressed at cellular and genetic levels further demanding the incorporation of effluent bioassays in risk assessment and risk management/abatement programs.

Effect of treated tannery effluent and domestic waste water irrigation on Tagetes erecta

ABSTRACT : Tanning industry is generating enormous quantities of effluent every day. The scientific ways and means of utilizing this liquid waste is of utmost important in reducing pollution load to the environment. Present study has been carried out to apply treated tannery effluent (TTE) diluted with domestic waste water (DWW) as irrigation sources at different concentration of 25 per cent, 50 per cent, 75 per cent and 100 per cent on a non food crop Tagetes erecta. Results reveal that application of diluted mixture of TTE along with DWW can be used efficiently for crop production. Germination percentage of 73.13 per cent was recorded with 25 per cent of TTE and 75 per cent of DWW dilution as compared with 71.17 per cent in control. Different growth parameters showed variation with different level of diluted tannery waste water application. Highest root and shoot length was observed in control (11.08 cm and 10.70 cm) followed by 25 per cent effluent irrigation (11.22 cm and 10.47 cm). Different concentrations of tannery effluent were found to influence the vigour index significantly. The highest vigour index was observed at control (1601.1) followed by 25 per cent effluent concentration (1585.59). The lowest vigour index was observed in case of treatment receiving 100 per cent effluent concentration (173.48). Red soil with different concentration of treated tannery effluent with domestic waste water recorded good results as compared to the black cotton soil.

Inbred mice strain shows neurobehavioral changes when exposed to tannery effluent.

The bovine leather processing (tanning industries) stands as a generating activity of potentially toxic waste. The emission of untreated effluents into the environment may cause serious harm to human and environmental health. Nevertheless, few studies have investigated the possible effects of intake of these effluents in experimental mammalian models. Thus, this study aimed to evaluate the neurobehavioral effects of chronic intake of different tannery effluent concentrations diluted with water (0.1, 1, and 5%) in male C57BL/6J mice. After 120days of exposure, the animals were subjected to different behavioral tests, predictive of anxiety (elevated plus maze (EPM), open-field (OF), and neophobia test), depression (forced swim), and memory deficits (object recognition test). From the EPM test, it was observed that the mice exposed to 0.1, 1, and 5% of tannery effluents showed higher anxiety scores compared to the animals in the control group. However, the results of this study revealed no differences among the experimental groups in the proportion (percentage) of locomotion in the central quarters/total locomotion calculated (by OF), considered an indirect measure for anxiety. At neophobia test, all the animals exposed to chronic intake of tannery effluents showed higher latency time to start eating, which corresponds to an anxiogenic behavior. Regarding the forced swim test, it was observed that the animals exposed to tannery effluents had longer time in immobility behavior, suggesting a predictive behavior to depression. Finally, the object recognition test showed that the treatments did not cause damage to the animals' memory. The recognition rate of the new object did not differ among the experimental groups. Thus, it is concluded that male C57BL/6J mice (inbred strain) exposed to tannery effluents have predictive neurobehavioral changes of anxiety and depression, without memory deficit.

Dermal exposure to tannery effluent causes neurobehavioral changes in C57Bl/6J and Swiss mice

Tannery effluents constitute highly polluting residues, which can cause negative impacts to people’s health and the environment. However, studies that have investigated the effects of the exposure to these xenobiotics on the central nervous system of mammal experimental models are rare, the few that have been published focusing on the exposure via oral intake (ingestion of water containing tannery effluent concentrations). In this sense, and with the objective of expanding the knowledge beyond the neurotoxic effects observed when water contaminated by these xenobiotics is ingested, the neurobehavioral effects of dermal exposure of male C57Bl/6J and Swiss mice were analyzed. The animals were exposed to raw (wet blue-type) tannery effluent for two hours during five days, totalizing 15 days of exposure. Afterwards, the animals underwent the elevated plus-maze (predictive of anxiety) and the object recognition tests (identification of memory deficit). Our data show that the dermal exposure to the tannery effluent caused an anxiogenic behavior in these animals, when compared those that did not have direct contact with these xenobiotics. It was also observed that the animals exposed to the tannery effluent obtained lower novel object recognition indices, thus evidencing memory deficit and indicating a possible influence of the tannery effluent constituents in animal cognition. The present study attests the hypothesis that dermal exposure to tannery effluents containing neurotoxic substances causes behavioral disorders in C57Bl/6J and Swiss mice.

Behavioral changes in female Swiss mice exposed to tannery effluents

Among the anthropic activities generating potentially toxic residues are those involved with bovine hide processing (tannery industries). However, knowledge is scant regarding the damage caused to the health of various organisms by tannery waste and studies are rare, especially in mammalian experimental models. This study therefore aimed to evaluate the physical and behavioral effects of the exposure of female Swiss mice to tannery effluent. To accomplish this, for a period of 15 days the animals were fed tannery effluent diluted with water in the following concentrations: 0% (control group, received only potable water), 5% and 10%. The body mass of the animals was evaluated at the beginning and end of the experiment, as well as the daily consumption of water and food. After 15 days of exposure to the effluent, the animals were submitted to the elevated plus maze (predictive of anxiety) and the forced swim test (predictive of depression). The treatments did not affect the animals’ body mass, either in eating behavior or in consumption of water. However, it was found that the animals that ingested tannery effluent concentrations of 5% and 10% exhibited an anxiolytic (lower level of anxiety, greater percentage of time in the open arms, longer time and frequency in the diving behavior, less time of lurks and less frequency of freezing) and an antidepressant effect (more time in climbing behavior and less time of immobility) when compared to the control group. It was concluded that the exposure of female Swiss mice to tannery effluents (5% and 10% diluted with water) causes behavioral changes, possibly related to the neurotoxicity of this waste, without causing physical changes in the animals.

Histopathological assessment of C57Bl/J mice organs exposed to tannery effluents

The effluent produced in tanneries can cause environmental damage and public health problems when disposed of improperly. However, few toxicological studies have evaluated the effects of the intake of tannery wastewater by mammals. The objective of this study is the histological assessment of organs of C57Bl/J mice exposed to the intake of different concentrations of raw tannery effluents, beginning with the hypothesis that these effluents can cause damage to the histological structure of the organs. The animals were divided into the following groups: control (0% effluent) and exposed to 0.1%, 1%, and 5% raw tannery effluent diluted in water, for a period of 120 days. For the histopathological evaluations, samples of the liver, kidney, spleen, heart, and lung were collected, fixed, and stained by the hematoxylin and eosin staining techniques. No anomalies were observed in kidney, spleen, heart, and lung fragments. Alterations were observed only in liver fragments. Moderate hydropic degeneration was detected in animals exposed to tannery effluents, mainly in the periportal space. A large number of necrotic hepatocytes (p<0.05) were detected, especially in animals exposed to higher tannery effluent concentrations. Further, the largest number of hepatocytes with karyomegaly (p<0.05) was observed in animals exposed to the highest effluent concentrations. Our study suggests that the observed alterations are related to the intake of tannery effluents and that these effluents cause changes that lead to an increase of free radical production and direct aggressions to hepatocyte membranes as well as to the appearance of hepatocellular karyomegaly.

Modulation of antioxidant defence system for detoxification of oxidative stress caused by tannery effluent in Eichhornia crassipes

A study was performed to evaluate capability of aquatic macrophyte Eichhornia crassipes plants to combat oxidative stress caused by tannery effluent treatment. For this purpose, tannery effluent was collected from “Up flow Anaerobic Sludge Blanket” (UASB) Jajmau, Kanpur. Plants of Eichhornia crassipes were exposed to various concentrations of tannery effluent (0.0, 25, 50, 75 and 100%) for 2 and 7 days durations. Plants accumulated significant (p&lt;0.01) amount of Cr (a major constituent of tannery effluent) in a concentration duration dependent manner; which was more in roots (220 mg g-1 dw) than in leaves (83 mg g-1 dw). Tannery effluent induced oxidative stress was evident by increased level of MDA content. To combat oxidative stress plants showed alleviated level of antioxidants as its defense mechanism. Among enzymatic antioxidants, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity increased over to control upto 2nd days of treatment however on exposure of long duration i.e. 7 days these antioxidants declined in correspond to various treatments. Similarly non enzymatic antioxidants (carotenoid and ascorbic acid content) which scavenged free radicals efficiently also showing elevation at all concentrations up to 2 days exposure over unstressed plants, thereafter responded in decreasing manner due to ill effects of treatments. Therefore it can be concluded, E. crassipes can be utilized as a biomonitoring as well as phytoremedial tool using aforesaid biomarkers for pollution caused by tannery effluent.DOI: http://dx.doi.org/10.3126/ije.v3i1.9947 International Journal of Environment Vol.3(1) 2014: 101-110

Impact of tannery effluent on germination of various varieties of wheat (Triticum aestivum L.)

The present study has been focused on the impact of tannery effluent on germination pattern of ten varieties of wheat (Triticum aestivum).The physico-chemical analysis of the tannery effluents showed that it had high salinity (45.97 ppt) and an acidic pH (3.61). For screening test, a setup with various concentrations of tannery effluent (4.5%, 9%, 13.5%and 18%) was prepared in petri plates to check the impact of tannery effluent on various varieties of wheat. Among ten varieties, PBW-343 and HS-365 showed better performance at 4.5% tannery effluentand considered to be more tolerant, whereas HS -295 was the most sensitive to tannery effluent treatment.

Heavy Metal Induced Antioxidant Defense System of Green Microalgae and its Effective Role in Phycoremediation of Tannery Effluent

Investigation of tannery effluent toxicology in green microalgae is of great importance from ecological point of view, because heavy metal has become a major contaminant in recent years. The present study determined the effect of various concentrations (0, 10, 25, 50, 75 and 100%) of heavy metals containing tannery effluent on cell growth and antioxidant defense system of two green microalgae. Treatment with effluent induced accumulation of reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). Lower tannery effluent concentrations increased algal growth, whereas higher concentration suppressed the growth and photosynthetic content. Both strains of the microalgae had proven effective in removing heavy metals from aqueous solutions with the highest removal efficiency being near 100% and it can be used for phycoremediation of wastewater in large scale.

Immunotoxicity of Tannery Effluent to the Freshwater Fish Cyprinous carpio

This study was undertaken to determine the effect of chronic exposure to sub-lethal concentrations of tannery effluent (TE) on the humoral antibody response and the cell-mediated immune response of the fish Cyprinous carpio. The LC(50) value of the TE for C. carpio was determined by bioassay to be 3.8%. Sub-lethal concentrations of TE (0.6% and 0.3%) significantly suppressed the humoral and cell-mediated immune responses. Exposure of C. carpio to the TE had a significant effect on mean acceptance time (MAT) for transplanted scales. MAT was found to be 5-8days for autografts and 4-7days for allografts. The somatic indices of the kidney and spleen were reduced compared with controls.

Evaluation of genotoxicity and cytotoxicity of treated tannery wastewater in southern Brazil

In this study, we analysed the genotoxic and cytotoxic effects of treated tannery wastewater using the micronucleus (MN) test in fish erythrocytes (Bryconamericus iheringii) and the Allium cepa test system. Fish were exposed to three different concentrations of tannery effluent (2, 5 and 10%) for 3 and 6 days. Onion bulbs were exposed to four dilutions of the effluent (2, 5, 10 and 25%) for 60 hours. The results indicated absence of genotoxicity in the treated tannery effluent; however, toxic compounds were identified, threatening the survival of fish species at the effluent discharge site.

Immune response in the tilapia, Oreochromis mossambicus on exposure to tannery effluent

The objective of the study was to investigate the effect of chronic exposure to sublethal concentrations of tannery effluent (TE) on the specific immune response and nonspecific immunity in tilapia, Oreochromis mossambicus. The effluent from the tannery was collected directly from a chrome-tanning factory situated in Dindigul district, Tamil Nadu, India. Apart from chromium (88.2 ppm), the effluent contained appreciable amount of calcium carbonate and sodium sulphate. Groups of fish (45–50 g) were exposed to 0.0053, 0.053 or 0.53% [0.1%, 1% or 10% LC 50] of TE for 28 days. The specific immune response of fish was assessed by antibody response to heat-killed Aeromonas hydrophila by ELISA and bacterial agglutination assay. Nonspecific immune mechanisms were assessed in terms of serum lysozyme activity, production of intracellular reactive oxygen species (ROS) and reactive nitrogen intermediates (RNI) by peripheral blood leucocytes (PBL). The results indicate that chronic exposure of fish to 0.53% of TE, significantly suppressed antibody response, nonspecific serum lysozyme activity, and ROS and RNI production. Exposure to 0.053% (1% LC 50) of TE also caused a similar suppressive effect though at a lesser degree. In conclusion, the study shows, that exposure to sublethal concentrations of TE, can lead to adverse effects on selected immune reactions in tilapia. Further, these findings may be important in terms of monitoring fish health and risk assessment during periods of fluctuating levels of pollutants in the natural and farm environments.

ORDERS

Tannery effluents constitute highly polluting residues, which can cause negative impacts to people’s health and the environment. However, studies that have investigated the effects of the exposure to these xenobiotics on the central nervous system of mammal experimental models are rare, the few that have been published focusing on the exposure via oral intake (ingestion of water containing tannery effluent concentrations). In this sense, and with the objective of expanding the knowledge beyond the neurotoxic effects observed when water contaminated by these xenobiotics is ingested, the neurobehavioral effects of dermal exposure of male C57Bl/6J and Swiss mice were analyzed. The animals were exposed to raw (wet blue-type) tannery effluent for two hours during five days, totalizing 15 days of exposure. Afterwards, the animals underwent the elevated plus-maze (predictive of anxiety) and the object recognition tests (identification of memory deficit). Our data show that the dermal exposure to the tannery effluent caused an anxiogenic behavior in these animals, when compared those that did not have direct contact with these xenobiotics. It was also observed that the animals exposed to the tannery effluent obtained lower novel object recognition indices, thus evidencing memory deficit and indicating a possible influence of the tannery effluent constituents in animal cognition. The present study attests the hypothesis that dermal exposure to tannery effluents containing neurotoxic substances causes behavioral disorders in C57Bl/6J and Swiss mice.

Comparative studies on accumulation of Cr from metal solution and tannery effluent under repeated metal exposure by aquatic plants: its toxic effects.

The present study demonstrates comparison of Cr accumulating potential by the plants of Najas indica Cham. (submerged), Vallisneria spiralis L. (rooted submerged) and Alternanthera sessilis R. Br. (rooted emergent) under repeated metal exposure and its effect on chlorophyll and protein concentrations. These plants were treated with different concentrations of Cr under repeated exposure in controlled laboratory conditions to assess the maximum metal accumulation potential. The plants of V. spiralis accumulated significantly high amount of Cr under laboratory conditions in comparison to N. indica and A. sessilis. The maximum accumulation of 1378, 458 and 201 microg (-1) dw Cr was found in the leaves of V. spiralis. N. indica and A. sessilis, respectively at 8 mg (-1) after 9 day of Cr exposure. These plants have shown a decrease in chlorophyll and protein concentrations with increase in Cr concentrations. In view of high accumulation of Cr in V. spiralis, the plants were treated with different concentrations of tannery effluent collected from Common Effluent Treatment Plant, Unnao (UP). The plants of V. spiralis treated with 100% tannery wastewater showed the maximum accumulation (57.5 microg g(-1) dw) of Cr in the roots after 10 days of exposure. The plants were found effective in removing Cr from solution and tannery effluent.