In order to determine the effect of genotype and environment on the concentration of carotenoids, tocopherols and phenolic compounds in narrow-leafed lupin a two-year study was conducted. The plant material comprised 55 breeding lines of Lupinus angustifolius. Quantitative concentration of carotenoids, tocopherols and phenolic compounds were determined using HPLC method. Mean carotenoid concentration amounted to 0.5 mg/100 g d.m. for violaxantin, 2.0 mg/100 g d.m. for lutein, 1.2 mg/100 g d.m. for zeaxantin and 0.5 mg/100 g d.m. for β-carotene, respectively. The share of the α-T homologue was 5 % (mean concentration 0.5 mg/100 g d.m.), while for the γ-T homologue it was 95 % (mean concentration 9.9 mg/100 g d.m.). The total concentration of phenolic acids in the tested lines of L. angustifolius ranged from 5.1 mg/100 g d.m. to 19.9 mg/100 g d.m. Among phenolic compounds a higher concentration of flavonoids was recorded, with their total level ranging from 100.7 mg/100 g d.m. to 152.8 mg/100 g d.m. Broad-sense heritability was 0.67–0.75 for carotenoids, 0.59–0.86 for tocopherols and 0.72–0.76 for phenolic compounds. Among all the analyzed genotypes, fifteen were selected as satisfactory and stable in terms of their concentration of biologically active compounds.