We report a case of nitrazepam poisoning in which the distribution of nitrazepam and 7-aminonitrazepam was determined in body fluids and tissues. A 52-year-old woman was found dead in a shallow ditch (approximately 5cm in depth), in the winter. Ambient temperature was 2–8°C. The postmortem interval was estimated to be approximately 1 day and no putrefaction was observed. The cause of death was thought to be drowning due to nitrazepam overdose and cold exposure. Blood concentrations of nitrazepam and 7-aminonitrazepam were very site dependent (0.400–0.973μg/ml and 0.418–1.82μg/ml). In addition, the concentration of the same analytes in the bile were 4.08 and 1.67μg/ml, respectively, and in the urine: 0.580 and 1.09μg/ml, respectively. A high accumulation of both substances was observed in various types of brain tissue (2.17–6.22μg/g and 2.49–5.11μg/g). Only small amounts of nitrazepam and 7-aminonitrazepam were detected in the liver (0.059 and 0.113μg/g, respectively). Large differences in the observed concentrations of nitrazepam and 7-aminonitrazepam among arterial and venous blood samples were thought to be mainly due to dilution of arterial blood by water entering the circulation through lungs at the time of death. Bacterial metabolism of nitrazepam may also have contributed to the observed differences.