Concentrations Of Aqueous Leaf Extracts Research Articles

Influence of Aqueous Leaf Extracts of Eucalyptus tereticornis Sm. On the Expression of Germination and Growth of Agricultural Seeds

Aim: This laboratory experiment was conducted to test the effect of allelopathy by Eucalyptus tereticornis aqueous leaf extracts on the germination and growth of Agricultural seeds i.e., Arachis hypogaea, Cicer arietinum, Vigna radiata and Zea mays as to test for suitable agroforestry system.
 Study Design: The experiment was conducted in a completely randomized block design.
 Place and Duration of the Study: The experiment was conducted in the laboratory of Agroforestry and Soil Science, Department of Silviculture and Agroforestry, Forest College and Research Institute, Mulugu, Siddipet, Telangana, India.
 Methodology: The aqueous leaf extracts of varied concentration [0 (Distilled water), 25, 50, 75 and 100% (m/v) %] prepared were tested against germination percent, relative germination percent, growth and relative allelopathic effect on the aforesaid agricultural seeds.
 Results: The bioassay revealed that there was significant turndown in parameters tested. Control being the one relatively high performing, with increase in the concentrations of aqueous leaf extracts the germination rate, root length, shoot length, and dry weights reduced in all the other crops treatments tested.
 Conclusion: The experiment attributes that leaching of allelochemicals from the leaves into the assay caused inhibitory effect on the seed germination and growth.

Effects of leaf extracts of Amorpha fruticosa on seed germination and seedling growth of Amygdalus pedunculata

Amorpha fruticosa and Amygdalus pedunculata are common plant species used for greening construction in arid and semi-arid region of Northwest China. In order to explore the feasibility of greening construction and ecological restoration by A. fruticose with A. pedunculata, we exami-ned the allelopathic effects of five concentrations of aqueous leaf extracts of A. fruticosa (0.025, 0.05, 0.10, 0.15 and 0.20 g·mL-1) on eight A. pedunculata varieties (YY1, YY3, YY4, YY5, YY6, SM6, SM7 and SM8), using the methods of paper-petri dish and soilless culture. The results showed that when the concentration of A. fruticosa leaf extracts were 0.025 and 0.05 g·mL-1, the seed germination and seedling growth of YY1 and SM6 were significantly better than other varieties. With increasing concentration of A. fruticosa leaf extracts, the catalase activity of A. pedunculata seedlings first increased and then decreased. The activities of peroxidase and superoxide dismutase, and the contents of soluble protein and chlorophyll showed a downward trend, while the contents of malondialdehyde and soluble sugar and the permeability of cell membrane gradually increased. Results of the principal component and cluster analysis showed that the growth potential of A. pedunculata decreased with the order of YY1, SM6, SM8, SM7, YY6, YY3, YY5 and YY4 under the allelopathic effect of A. fruticose. In conclusion, the artificial collocation and mixed planting of low-density of A. fruticosa with YY1 and SM6 were beneficial to seed germination and seedling growth of A. pedunculata.

Toxicity of aquaeous leaf extracts of Gongronema latifolium on behaviourial and haematological characteristics of Clarias gariepinus (Burchell, 1822) juveniles

A static bioassay experiment was set up to investigate effects of acute (96-hrLC50) concentrations of aqueous leaf extracts of Gongronema latifolium on behaviourial and haematological characteristics of Clarias gariepinus (Burchell, 1822) juveniles. Evaluation of toxicity of leaf extracts at concentrations of: 0, 10, 25, 50, 75, and 100mg/l was done at various time intervals on the fish. A total of 180 catfish juveniles with average initial weight of 8.37±1.36g and total length of 12.10±2.45cm were used. The fish were distributed among the treatments on complete randomized design. Each treatment had three replicates. The results showed that aqueous leaf extracts were toxic to experimental fish. There was an observed erratic movement, colour change from black to ash-brown and dark coloured belly of treated fish. There were slight changes in water quality parameters, with no significant effect (p>0.05) on the tolerance level of fish on G. latifolium leaf extracts. Mean values of 96-hrLC50 were 90.14mg/l and 2.78mg/l for the upper and lower limits while the threshold value was 0.26mg/l. Phyto-chemical analysis of the leaves showed constituents of flavonoids, saponins, tannins, anthrocyanide, alkaloids and glycosides. Effects of aqueous extracts on haematological indices revealed increasing values of pack cell volume, haemoglobin, white blood cell, red blood cell, mean corpuscular volume, mean corpuscular heamoglobin and mean corpuscular heamoglobin concentration counts with extract concentration. Thus, G. latifolium could be used as feed additive for improved fish production at concentration below 70%.
 Keywords: Gongronema latifolium, leaf extracts, behaviour, haematology, Clarias gariepinus

Evaluation of Hyptis spicigera leaf extracts against cowpea weevil (Callosobruchus maculatus) in stored Bambara groundnut (Vigna subterranea L.)

Laboratory study was conducted at the University for Development Studies, Tamale, Ghana in 2016 to evaluate the potency of different concentrations of aqueous leaf extracts (ALE) from Hyptis spicigera (Lam.) against Callosobruchus maculatus (F.) infestation in Bambara groundnut seeds. Two aqueous extracts concentrations were prepared at three temperatures. The treatments were; control (sterilized untreated seed), 10% ALE [w\v] applied at 25 °C, 10% ALE applied at 50 °C, 10% ALE applied at 100 °C, 20% ALE applied at 25 °C, 20% ALE applied at 50 °C and 20% ALE applied at 100 °C. The results showed that, application of 20% ALE at 100 °C recorded a significant (p < 0.05) lower number of eggs (26.3), adults emerge (6.5), seed damage (1.6%), seed weight loss (1.1%), susceptibility index (0.4%) and a higher inhibition rate (2.3%) compared to the control. Seed viability test was also highest (89.3%) in the 20% ALE at 50 °C. The formulation of 20% ALE at 100 °C was the most potent against C. maculatus infestation. Seeds destined for human consumption can be treated at this concentration and temperature. We also recommend 20% ALE at 50 °C for protecting seeds stored for propagation.

Synthesis of biologically active copper oxide nanoparticles as promising novel antibacterial-antibiofilm agents

In this study, we aimed to synthesize copper oxide nanoparticles (CuONPs) mediated by plant extract in an environmentally friendly way and to reveal their potential biological activities. Here we synthesized CuONPs by using different concentrations of aqueous leaf extract of Thymbra spicata at 80 °C to obtain Ts1CuONPs and Ts2CuONPs. Biosynthesized nanoparticles were characterized by using UV-Vis, AFM, FTIR, SEM-EDS, TEM, DLS and zeta potential analysis. The antibacterial activity of the nanoparticles was determined by calculation of the inhibition zone and minimum inhibitory concentration against selected bacterial strains. Moreover, the antioxidant activity of the as-synthesized nanoparticles was evaluated based on DPPH radical scavenging activity. The results indicate that the as-synthesized NPs have an average size of 26.8 and 21 nm for Ts1CuONPs and Ts2CuONPs, respectively. The formed CuONPs have more antibacterial action on gram-positive bacteria compared to gram-negative bacteria. In addition, CuONPs demonstrated good inhibition activity against biofilm formation of Staphylococcus aureus (S. aureus). Furthermore, the results showed that the smaller size of the CuONPs caused the higher cytotoxicity on L929 mouse fibroblast cells. The as-synthesized CuONPs exhibit antibacterial and antibiofilm potential against S. aureus, indicating that they may be attractive candidates to use in future therapeutic applications.

Insecticidal Activities of the Leaf Extract of the Invasive Alien Plant, Chromolaena odorata (L.) (Asteraceae) Against Macrotermes species (Termites)

Several studies have demonstrated the biological efficacy of leaf, stem and root powders or extracts of Chromolaena odorata (L.) King and Robinson against insect pests but those that are focused on the biological efficacy of aqueous leaf extracts against Macrotermes species are scanty. Current management of termites with synthetic insecticides is being discouraged due to human and environmental hazards. Therefore, the insecticidal effectiveness of aqueous leaf extract C. odorata against Macrotermes species was investigated. Five concentrations (0, 2.5, 5.0, 7.5 and 10.0% (w/v)) of the aqueous extract of C. odorata plant were evaluated for repellency and toxicity on the worker caste of Macrotermes species following standard procedures. The filter paper impregnation technique was used for the bioassay. Percentage repellency was monitored for 30 minutes and mortality recorded at 12, 24 and 36 hours post exposure. The leaf extract of C. odorata significantly repelled 95% of Macrotermes species at the highest concentration of 10% (w/v) after 30 minutes post treatment exposure. Mortality of Macrotermes species was independent of treatment concentration, but dependent on duration of exposure. All treatment concentrations of aqueous leaf extract of C. odorata caused significant mortality against Macrotermes species ranging between 94% and 98% compared to the control; indicating very great potential for adoption and use in the management of Macrotermes species.

Nematicidal Activity of Lantana Camara L. for Control of Root-Knot Nematodes

Various concentrations of aqueous leaf extract of Lantana camara L. were assessed against second stage juveniles (J2) of Meloidogyne spp. (Goeldi, 1982) for its nematicidal potency in vitro conditions. Study showed 50% concentration of Lantana camara leaf extract at 48 hrs of incubation period and above showed effective in immobilizing second stage of larvae (J2) of Meloidogyne spp. The standard concentration ‘S’ (100%) of leaf extract was found to be highly nematostatic, 98.66% of nematode were found dead in 48 hrs. Similarly, 57.66% of nematode juveniles were found dead when applied 50% concentration in 48 hrs. Mean number of (J2) dead at 100% concentration for three time period was statistically significant highest at 48 hrs. So far, 50% concentration in 48 hrs and above was appropriate for controlling the root-knot nematode which seems as an alternative to chemical pesticides.

Recent perspectives on the anticancer properties of aqueous extracts of Nigerian Vernonia amygdalina.

Innovative developments are necessary for treating and defeating cancer, an oftentimes deadly group of diseases characterized by the uncontrolled growth and spread of abnormal cells. Breast cancer (BC) is the second leading cause of cancer-related deaths of women in the USA, and prostate cancer (PC) is the second leading cause of cancer-related deaths of American men. Although some efficacious BC drugs are pharmaceutically marketed, they affect the quality of life for some patients because they are toxic in that their usages have been accompanied by side effects such as stroke, thrombosis, slow heart rate, seizure, increased blood pressure, nausea, emesis, and more. Therefore, there is an urgent need for the discovery of molecular markers for early detection of this disease and discovery of targets for the development of novel, less toxic therapeutics. A botanical plant Vernonia amygdalina has been widely used in Nigerian and other Central and West African cultures for centuries as an herbal medicine. Mounting evidence suggests that treatment with low concentrations of aqueous leaf extracts of the edible Nigerian V. amygdalina plant (Niger-VA) arrests the proliferative activities and induces apoptosis in estrogen receptor-positive, estrogen receptor-negative, and triple-negative human breast cancerous cells and in androgen-independent human PC-3. Also, in athymic mice, Niger-VA potentiates increased efficacies and optimizes treatment outcomes when given as a cotreatment with conventional chemotherapy drugs. Evidence of its noticeable cytostatic activities ranging from changes in DNA synthesis to growth inhibition, mechanisms of inducing apoptosis in different cancer cell lines, and in vivo antitumorigenic activities and chemopreventive efficacy reinforce the idea that Niger-VA deserves increased attention for further development as a phytoceutical, anticancer drug entity. Hence, the present review article highlights impactful published literature on the anticancer effects of Niger-VA in multiple cancerous cell lines and in a nude mouse model, supporting its potential usefulness as a natural product, chemotherapeutic medicine for treatment of both BC and PC.

Effect of Different Concentrations of Aqueous Leaf Extracts of Some Plants on the Germination and Seedling Growth of Maize, Z. mays L. and Wheat (T. aestivum L.)

Allelopathic effect of aqueous leaf extract of three different species (eucalyptus, guava, and litchi) were used to investigate their effect on germination, shoot and root lengths of two food crops viz. Zea mays L. (Maize) and Triticum aestivum (Wheat). Mature fresh leaves of three species were crushed and soaked for 24h; the filtrates were diluted to make different concentrations (T1 = 0.5%, T2 = 1.0%, T3 = 1.5%, T4 = 2.0%, and T5 = 0.0%), and were used to investigate their effect on the tested crops. The aqueous leaf leachate of three species was found to have inhibitory effect on germination, shoot, and root elongation on the tested crops. Current study indicates that inhibitory effect was much more pronounced at higher concentrations, and the effect increased with the increase in concentration. Among trees, Eucalyptus has much more effect on the tested crops than other species. DOI: http://dx.doi.org/10.3126/ije.v3i3.11085 International Journal of Environment Vol.3(3) 2014: 264-274

English

Wide usage of Waltheria indica plant has been reported but its effects on the reproductive indices of male albino rats have not been evaluated. This study investigates the effects of W. indica leaf extract on the sperm counts, motility, live-dead ratio and sperm morphological abnormality of male albino rats. Thirty six (36) healthy white male albino rats were randomly selected and divided into six groups. Each of the treated groups was administered with different concentrations of aqueous leaf extract for 21 days after which the semen samples were taken for analysis of reproductive parameters. The testicles were also removed for histological study. There was significant decrease (p&lt;0.01) in sperm motility and sperm counts at doses 800, 1600 and 2000 mg/kg. The live-dead ratio was also significantly decreased (p&lt;0.01) at 2000 mg/kg dose. There was also significant increase in total abnormal cell for all the treated groups. The extract induced periportal cellular infiltration and interstitial congestion on the testes of the treated rats. &nbsp; Key words: Waltheria indica, motility, sperm counts, live-dead ratio, abnormal cell, histology, rats.

Acacia holosericea (Fabaceae) litter has allelopathic and physical effects on mission grass (Cenchrus pedicellatus and C. polystachios) (Poaceae) seedling establishment

Invasion of grass weeds is a major threat for ecosystems. Mission grass (Cenchrus pedicellatus and C. polystachios) vigorously competes with native vegetation and has become a serious problem in northern Australian savanna. A lower density of mission grass has been observed under the canopy of stands of native Acacia holosericea. We used a series of laboratory and shade house experiments to assess the potential for allelopathy and the role of litter on germination, emergence and seedling growth of these two species of mission grass. Different concentrations of aqueous leaf extract of A. holosericea were used to assess allelopathic effects on germination. Various depths and types of litter were used to investigate the allelopathic and physical effects of litter on emergence and growth of mission grass seedlings in the shade house. Results indicate that extracts did not affect germination of either species of mission grass but root growth of seedlings was affected. Emergence of seedlings in the shade house was affected by physical litter treatments but not by allelopathy. After emergence no negative effects on seedling growth were detected. Overall we found that there was no allelopathic effect on germination and that the negative effect on emergence was due to the physical properties of the litter. This effect on emergence increased with increasing depth of litter. Allelopathy slightly inhibited root growth but once seedlings emerged, litter tended to facilitate growth. This has implications for the ecological management of mission grass on disturbed lands, using strategies such as manipulation of litter cover through Acacia establishment.

Evaluation of Catharanthus roseus leaf extract-mediated biosynthesis of titanium dioxide nanoparticles against Hippobosca maculata and Bovicola ovis

The purpose of the present study was based on assessments of the antiparasitic activities of synthesized titanium dioxide nanoparticles (TiO(2) NPs) utilizing leaf aqueous extract of Catharanthus roseus against the adults of hematophagous fly, Hippobosca maculata Leach (Diptera: Hippoboscidae), and sheep-biting louse, Bovicola ovis Schrank (Phthiraptera: Trichodectidae). The synthesized TiO(2) NPs were analyzed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and atomic force microscopy (AFM). The formation of the TiO(2) NPs synthesized from the XRD spectrum compared with the standard confirmed spectrum of titanium particles formed in the present experiments were in the form of nanocrystals, as evidenced by the peaks at 2θ values of 27.43°, 36.03°, and 54.32°. The FTIR spectra of TiO(2) NPs exhibited prominent peaks at 714 (Ti-O-O bond), 1,076 (C-N stretch aliphatic amines), 1,172 (C-O stretching vibrations in alcoholic groups), 1,642 (N-H bend bond), and 3,426 (O-H stretching due to alcoholic group). SEM analysis of the synthesized TiO(2) NPs clearly showed the clustered and irregular shapes, mostly aggregated and having the size of 25-110 nm. By Bragg's law and Scherrer's constant, it is proved that the mean size of synthesized TiO(2) NPs was 65 nm. The AFM obviously depicts the formation of the rutile and anatase forms in the TiO(2) NPs and also, the surface morphology of the particles is uneven due to the presence of some of the aggregates and individual particles. Adulticidal parasitic activity was observed in varying concentrations of aqueous leaf extract of C. roseus, TiO(2) solution, and synthesized TiO(2) NPs for 24 h. The maximum parasitic activity was observed in aqueous crude leaf extracts of C. roseus against the adults of H. maculata and B. ovis with LD(50) values of 36.17 and 30.35 mg/L, and r (2) values of 0.948 and 0.908, respectively. The highest efficacy was reported in 5 mM TiO(2) solution against H. maculata and B. ovis (LD(50) = 33.40 and 34.74 mg/L; r (2) = 0.786 and 0.873), respectively, and the maximum activity was observed in the synthesized TiO(2) NPs against H. maculata and B. ovis with LD(50) values of LD(50) = 7.09 and 6.56 mg/L, and r (2) values of 0.880 and 0.913, respectively. This method is considered as an innovative alternative approach to control the hematophagous fly and sheep-biting louse.

Acaricidal efficacy of synthesized silver nanoparticles using aqueous leaf extract of Ocimum canum against Hyalomma anatolicum anatolicum and Hyalomma marginatum isaaci (Acari: Ixodidae)

The use of acaricides had limited efficacy in reducing tick infestations and is often accompanied by serious drawbacks, including the selection of acaricide resistant ticks, contamination of environment, and milk and meat products with drug residues. The present study was based on assessments of the antiparasitic activities to determine the efficacy of synthesized silver nanoparticles (AgNPs) utilizing aqueous leaf extract of Ocimum canum Sims (Labiatae) against the larvae of Hyalomma anatolicum (a.) anatolicum Koch, 1844 and Hyalomma marginatum (m.) isaaci Sharif, 1928 (Acari: Ixodidae). The synthesized AgNPs results were recorded from UV-vis spectrum, X-ray diffraction (XRD) analysis, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis. The production of the AgNPs synthesized from the leaf extract of O. canum was evaluated through UV-visible spectrophotometer in a range of wavelength from 300 to 600 nm. This revealed a peak at 426 nm in leaf extracts of O. canum, indicating the production of AgNPs. The XRD spectrum compared with the standard confirmed spectrum of silver particles formed in the present experiments were in the form of nanocrystals, as evidenced by the peaks at 2θ values of 27.71°, 32.16°, 38.08°, 46.15°, 54.70° and 57.35°. The FTIR spectra of AgNPs exhibited prominent peaks at 818, 1,045, 1,381 and 1,616 in the region 500-3,000 cm(-1). The peaks correspond to the presence of a C-H vibration of the aromatic ring, stretch vibration of C-O, carbonyl groups and flavanones. SEM analyses of the synthesized AgNPs were clearly distinguishable, which measured 25-110 nm in size. It is clear that the rod and cylindrical structures have an average size of 95 nm. The EDX spectra showed the purity of the material and the complete chemical composition of the synthesized AgNPs. Parasite larvae were exposed to varying concentrations of aqueous leaf extract of O. canum and synthesized AgNPs for 24 h. The acaricidal activities of aqueous crude leaf extracts of O. canum against the larvae of H. a. anatolicum and H. m. isaaci have LC(50) and LC(90) values of 15.31 and 13.85 mg/L, and 62.41 and 48.86 mg/L, respectively. The efficacies of 1 mM AgNO(3) solution against H. a. anatolicum and H. m. isaaci were LC(50) = 12.25 and 12.17 mg/L, LC(90) = 49.17 and 46.52 mg/L, respectively, and the maximum efficacy was observed in the synthesized AgNPs against H. a. anatolicum and H. m. isaaci with LC(50) and LC(90) values of 0.78 and 1.00 mg/L, and 1.51 and 1.68 mg/L, respectively. This method is considered as an innovative alternative approach to control parasites.

Antioxidant activities of aqueous leaf extracts of Toona sinensis on free radical-induced endothelial cell damage

In Taiwan, Toona sinensis (Toona sinensis) is well known as a traditional Chinese medicine, while the underlying pharmacological mechanisms of this drug are still a matter of debate. The purpose of this study was to evaluate the protective effects of non-cytotoxic concentrations of aqueous leaf extracts of Toona sinensis (TS extracts; 50-100 μg/mL) and gallic acid (5 μg/mL), a major component of these extracts, against AAPH-induced oxidative cell damage in human umbilical vein endothelial cells (ECs). Exposure of ECs to AAPH (15 mM) decreased cell viability from 100% to 43%. However, ECs were pre-incubated with TS extracts prior to AAPH induction resulted in increased resistance to oxidative stress and cell viability in a dose-dependent manner. An increase in ECs-derived PGI(2) and IL-1 β in response to AAPH exposure was positively correlated with cytotoxicity and negatively with TS extracts concentrations. In addition, gallic acid also suppressed PGI(2) and IL-1 β production in AAPH-induced ECs. Notably, TS extracts/gallic acid treatment significantly inhibited ROS generation, MDA formation, SOD/catalase activity, and Bax/Bcl-2 dysregulation in AAPH-stimulated ECs. Pretreatment of ECs with TS extracts/gallic acid also suppressed AAPH-induced cell surface expression and secretion of VCAM-1, ICAM-1 and E-selectin, which was associated with abridged adhesion of U937 leukocytes to ECs. Moreover, TS extracts/gallic acid treatment significantly inhibited the AAPH-mediated up regulation of PAI-1 and down regulation of t-PA in ECs, which may decrease fibrinolytic activity. Therefore, Toona sinensis may possess antioxidant properties that protect endothelial cells from oxidative stress. Our results also support the traditional use of Toona sinensis in the treatment of free radical-related diseases and atherosclerosis.

Nematicidal activity of leaf extracts from Lantana camara L. against Meloidogyne incognita (kofoid and white) chitwood and its use to manage roots infection of Solanum melongena L.

Various concentrations of aqueous leaf extract of Lantana camara were assessed in vitro conditions against second stage juveniles (J2) of Meloidogyne incognita. The standard concentration 'S' of leaf extract was found to be highly nematostatic, where nematodes were completely paralyzed after 12 h and after 48 h of exposure, 96% of juveniles were killed at same concentration. However, the mortality of juveniles was 75% in S/2 dilution at 48 h. The degree of effectiveness and dilutions of extract were directly proportional. The percentage of paralyzed juveniles was decreased, when J2 transferred in distilled water after 48 h incubation in standard aqueous leaf extract. Addition of freeze-dried aqueous extract to sterile sandy substrate at S/2 dilution significantly decreased the root-knot infection to susceptible eggplants whereas second stage juveniles (J2) that penetrated roots of eggplant were able to complete development in sterile sandy substrate without treatment of freeze-dried aqueous extract.

Cytotoxicity testing of aqueous extract of bitter leaf (Vernonia amygdalina Del.) using the Allium cepa chromosome aberration assay.

Mitotic studies were carried out on Allium cepa (onion) root cells treated with different concentrations of aqueous leaf extract of Vernonia amygdalina (bitter leaf). The onion root cells showed reduced mitotic indices with corresponding increase in concentration of the bitter leaf extract [200 g/L (C2), 400 g/L (C3) and 500 g/L (C4)]. Chromosomal aberrations, such as endopolyploidization, lagging of chromo-somes and cells with giant chromosomes, were also observed in onion roots treated with same. No chromosomal aberration was observed in the control and in onion roots treated with 100 g/L (C1) of the leaf extract. These observations indicate that abnormal use of this medicinal herb could cause genetic damage. Low concentration and wide spacing of dosage are, therefore, suggested for its dietary intake or use in herbal medicine. Key words: Vernonia amygdalina, Allium cepa, chromosome aberration, cytotoxicity, herbal medicine.

Allelopathic effects of Lantana camara on germination and growth behavior of some agricultural crops in Bangladesh

An experiment was conducted to understand the growth inhibitory effects of aqueous extracts derived from Lantana camara L. (a globally recognized invasive alien weed) on six popular agricultural crops of Bangladesh. The test was conducted in sterilized petridishes with a photoperiod of 24 hours and an average temperature of 29°C. The effect of different concentrations of L. camara leaf extracts were recorded and compared with control (i.e., distil water). Result showed different concentrations of aqueous leaf extracts caused significant inhibitory effect on germination, root and shoot elongation and development of lateral roots of receptor crops. Bioassays also indicated that the inhibitory effect was proportional to the concentrations of the extracts and higher concentration had the stronger inhibitory effect whereas the lower concentration showed stimulatory effect in some cases. The inhibitory effect was much pronounced in root and lateral root development rather than shoot and germination.