Concentration In Nutrient Medium Research Articles

Impact of Acadian Marine Plant Extract Powder (AMPEP) concentration in nutrient medium on the growth and lipid accumulation of Chlorella sp. Culture

Microalga of the genus Chlorella has developed a stable industry as dietary supplements for humans and animals based on their scientific and commercial interests. The growth of Chlorella sp. cultures has been enhanced by using a variety of nutrients to enhance pigmentation, lipid content, and growth. The use of Acadian Marine Plant Extract Powder (AMPEP) improves agricultural crops and macroalgae production, such as seaweeds. However, AMPEP has not yet been studied as a means of producing microalgae. Therefore, this study investigates microalgae production in a nutrient medium containing AMPEP. Three concentrations of AMPEP were prepared: group A (125 mg L-1 AMPEP), group B (625 mg L-1 AMPEP), and group C (0 mg L-1 AMPEP) as control. Experiments were conducted for each group for 21 days in triplicate. Results revealed that lower AMPEP (125 mg L-1) concentration added to the nutrient medium provides higher cell densities in Chlorella sp. culture. 125 mg L-1 AMPEP in a nutrient medium reached the highest cell density of 1.28 fold cell mL-1 than the control group. Additionally, the dry weight of groups A, B, and C were calculated as 2.57 ± 0.12 g L-1, 1.37 ± 0.06 g L-1, and 1.58 ± 0.16 g L-1, respectively. The cell size of groups A, B, and C were 4.80 ± 1.32 μm, 5.20 ± 1.87 μm, and 3.80 ± 0.79 μm, respectively. Moreover, the highest level of lipid accumulation of Chlorella sp. culture was achieved by group B with a lipid content of 10.44 ± 1.28 %, followed by group A with a lipid content of 8.55 ± 0.80 %, which was higher than the control group (group C) with a lipid content of 7.04 ± 0.93 %. Hence, the present study shows that AMPEP used in microalgae production may improve growth and lipid accumulation.

The Influence of cobalt concentration in nutrient medium on growth of California worm biomass and metal accumulation in it

The biomass of the California redworm hybridis abiologically valuable feed additive for farm animals, poultryand fish. Worms contain a large amount of protein, lipids,vitamins and trace elements. The chemical composition ofworm biomass depends on the nutrient medium on whichit was grown. Worms are able to accumulate trace elementsfrom the nutrient medium in their body. Using this property,we conducted research on the biomass of worms enrichedwith Cobalt with the prospect of its furthe rusein fishfeeding. It was found that increasing the number and weightof worms depends on the content of Cobalt in the nutrientmedium. The in troduction of 20 mg/kg of Cobalt in to thenutrient medium increased the number and weight of wormsweighing 0.4-0.8 g, respectively, by 38.0% and 40.4%. Anincrease in the number of immature worms by 32.2% wasdetected. By adding 40 mg/kg of Cobalt to the nutrientmedium, the umber and weight of worms increase by 45.9and 51.1%, respectively. The introduction of Cobalt in theamount of 160 mg/kg resulted in a decrease in the numberof worms weighing 0.4-0.8 g compared to the experimentalgroups by 6.5-27.7%. It was found that at a dose of Cobalt160 mg/kg, the number of small worms decreased relativeto the experimental groups by 24.0-50.7%. There was adecrease and weight of young worms relative to control by22.4%.It is established that with increasing cobalt content in thenutrient medium increases its concentration in the biomassof worms. The highest content of Cobalt was found in thedrymatter of the biomass of worms grown on a nutrientmedium to which was added the test metalin the amount of160 mg/kg.The biomass of worms grown on a nutrient medium towhich was added 40 mg/kg of the investigated metal canbeuse das a protein supplement with high cobalt content infish feeding. Key words: vermiculture, wormbiomass, metalaccumulation, nutrient medium, Cobalt, atomicabs orptionspectros copy.

Establishment of in vitro culture, plant regeneration, and genetic transformation of Camelina sativa

The results on in vitro culture establishment, plantlet regeneration and rooting of Camelina sativa cultivar sample Peremozhets and cultivar Mirazh are presented. Effective concentrations of sterilizing agents and duration of plant material treatment were estimated. Phytohormone ratio, sucrose concentration in nutrient medium that induce effective formation of C. sativa shoots and NAA concentration for plantlet rooting have been established. The method of Agrobacterium-mediated transformation of Camelina by using binary vector pGH217 carrying reporter beta-glucoronidase (gus) gene driven under 35S CaMV promoter and nos-terminator, and selective marker hpt gene conferring hygromycin-resistance in transgenic plant was elaborated.

Effects of genotype, explant type and nutrient medium components on canola ( Brassica napus L.) shoot in vitro organogenesis

The objective of the study was to develop an efficient method for shoot regeneration of canola (Brassica napus L.) and to compare the regeneration capacity of different explants on MS medium with several combinations of plant growth regulators. The experiments showed that the morphogenetical potential of canola depends on genotype, primary explant, hormonal structure and concentration of nutrient medium. Cotyledons possessed higher regeneration ability in comparison to hypocotyls and roots. The best regeneration capability was exhibited by the cultivar 'Quantum'. Its frequency with cotyledonary explants reached 68.8% on all used media. Addition of 3 mg/l ABA in nutrient medium considerably increased the regeneration frequency. The highest shoot regeneration (100%), however, took place when cotyledonary explants were cultivated on medium, containing 1.0 mg/l NAA, 8.0 mg/l BAP and 3.0 mg/l ABA. Precultivation of explants on callus induction medium did not affect the shoot regeneration frequency. Vitrification of regenerants was promoted by increasing the auxin NAA or cytokinin BAP, and ABA in the nutrient medium.

Effects of genotype, explant type and nutrient medium components on canola ( Brassica napus L.) shoot in vitro organogenesis

The objective of the study was to develop an efficient method for shoot regeneration of canola (Brassica napus L.) and to compare the regeneration capacity of different explants on MS medium with several combinations of plant growth regulators. The experiments showed that the morphogenetical potential of canola depends on genotype, primary explant, hormonal structure and concentration of nutrient medium. Cotyledons possessed higher regeneration ability in comparison to hypocotyls and roots. The best regeneration capability was exhibited by the cultivar 'Quantum'. Its frequency with cotyledonary explants reached 68.8% on all used media. Addition of 3 mg/l ABA in nutrient medium considerably increased the regeneration frequency. The highest shoot regeneration (100%), however, took place when cotyledonary explants were cultivated on medium, containing 1.0 mg/l NAA, 8.0 mg/l BAP and 3.0 mg/l ABA. Precultivation of explants on callus induction medium did not affect the shoot regeneration frequency. Vitrification of regenerants was promoted by increasing the auxin NAA or cytokinin BAP, and ABA in the nutrient medium.

The function of metabolism in phosphorus accumulation in plant roots and its transport over long distances

The values of influx (Ji) and efflux (Jo) of phosphates through intact maize roots (primary, seed roots) have confirmed the dependence of the P concentration in nutrient medium on the activity and efficiency of transport mechanism with respect to the accumulation of phosphates (J) by roots. The phosphate accumulation is about 97–99 % of the total uptake. If the P concentration is < 1 mM the efflux is negligible, and Ji <=g Jo. In contrast, if the P concentration is τ 1 mM, the proportion of efflux significantly increases, up to 45 % of the whole influx. The approximation to the conditions of equilibrium of phosphate flows ( Ji = Jo) depends on the P concentration in root cells, the accumulation of phosphates being determined by the relation Ji τ Jo. In the roots growing in P-containing medium the values of efflux are much higher than in the roots lacking P. The positive effect of Ca2+ ions on the accumulation of phosphates is caused by the decreased proportion of efflux. The factors instigating the integrity or non-integrity of the cell structure (Ca2+, SDS, EDTA, Sorbitol,etc.) and thus its effectiveness determine the accumulation of phosphates by roots. Analogously, the factors stimulating the ability of accepted phosphate to be metabolized, and their use in the form of organic compounds decrease the proportion of efflux; these activities are shown in the increased efficiency of the phosphate uptake. The presented results show the importance of the integrity of the cell structure, the functioning of membranes and of metabolism efficiency for the accumulation of phosphates by plant roots. The main form of phosphorus transport in xylem exudate is inorganic phosphorus. Its share is from 79 to 82 % of the total amount of transported P. The utilization of P in the roots in the form of organic, slowly motabolizable P compounds (mannose-6-phosphate) and inhibition of acid phosphatase activity effectively restrains P transport over long distances. The correlation of P transport from roots into shoots with phosphatase activity was established (correlation coefficient is 0.74++). It can be summarized that long-distance P tran sport is a function of dephosphorylating reactions.

Microprobe techniques for determining diffusivities and respiration rates in microbial slime systems

AbstractA microprobe electrode was used to determine dissolved oxygen concentrations near the surface and within a bacterial slime mass supplied with a continuous flow of nutrient solution. With dilute medium, the oxygen profile became level at high concentrations within the film, indicating substrate‐limited respiration. More concentrated medium caused the profile to fall to low oxygen concentrations characteristic of oxygen‐limited respiration. Oxygen responses to sudden changes in concentration of nutrient medium were measured. Estimates of microbial respiration rate and of diffusivity of oxygen were based on well‐known diffusion equations.

接木植物のリン酸吸収 : リン酸濃度に対する反応 : 接木植物の栄養生理学的研究(第11報)

接木植物のリン酸吸収 : リン酸濃度に対する反応 : 接木植物の栄養生理学的研究(第11報)