Introduction:Staphylococcus aureusis the most important causative agent of wound infections, including diabetic foot ulcers. Honey is a very useful nutrient with antimicrobial properties and other biological properties such as antitumor, anti-inflammatory, antioxidant and antiviral properties. The aim was to examine the antibacterial activity of honey against methicillin-resistant and sensitiveS. aureus(MRSA and MSSA) isolated from patients with diabetic foot ulcers.Methods:This cross-sectional study was performed from January 2019 to December 2019. TwentyS. aureusisolates were collected from patients with diabetic foot ulcers. Different concentrations (100%, 70%, 50%, 25% vol/vol) of honey were studied. Dilutions of honey solutions were examined to determine the minimum inhibitory concentration (MIC) againstS. aureus. MICs were determined by spectrophotometric assay at 620 nm.Results:All strains showed sensitivity to honey with MIC equal to 25% (vol/vol). The MIC (%) values of honey for all studiedS. aureus (MRSA and MSSA) isolates ranged between 18-100% (v/v).Conclusion:Honey with confirmed, antibacterial activity has the potential to be an efficient treatment complementary for diabetic foot ulcers infected or at risk of infection withS. aureus.