Concentration Of Coconut Water Research Articles

Synthesis of sandwich structure forward osmosis membrane with calcium-carboxyl modified polyamide and tannic acid-Fe3+ interlayer and its application in coconut water concentration

In this study, a sandwich structure thin-film composite forward osmosis (TFC-FO) membrane based on calcium-carboxyl interfacial polymerization was synthesized using tannic acid-Fe (TA-Fe) as the interlayer. Additionally, the concentration effect of Ca2+ surface modification, TA-Fe intermediate layers, and their combination on coconut water concentration, membrane efficiency, anti-fouling ability, and quality of the concentrate was investigated. Compared to the TFC-Ca or TFC-TA-Fe membrane, the TFC-TA-Fe-Ca membrane significantly enhanced the concentration effect and reverse salt flux (p < 0.05) and exerted the lowest attenuation of permeability. Furthermore, the total soluble solids content increased from 5.13 °Brix to 13.17 °Brix after 8 h. The TFC-TA-Fe-Ca membrane showed a 61.20 % increase in its efficiency compared to the TFC membrane. The total reducing sugar, acids, and polyphenol contents increased by 1.72, 1.34, and 1.22 times, respectively. Overall, the sandwich structure FO membrane exhibited significant feasibility and potential for industrial application in fruit juice concentrate production.

Forward Osmosis for Concentration of Coconut Water

Forward Osmosis for Concentration of Coconut Water

Effects of Coconut Water and Banana Homogenate on Shoot Regeneration of Meyer Lemon (Citrus × meyeri)

Meyer lemon (Citrus × meyeri) is a hybrid citrus fruit from the Rutaceae family, originating from China. It is well-known for its distinctive appearance and flavor, as well as its health-nourishing nutrients. Micropropagation is an efficient alternative in the multiplication of plant stocks suitable for the commercial scale. The inclusion of organic additives in culture media has been found to provide a cost-effective option as a plant growth stimulant for in vitro plant development. The current study intends to assess the effects of coconut water and banana homogenate in the regeneration of Meyer lemon. In vitro, shoots were treated in half-strength Murashige and Skoog media fortified with 2 mg/L 6-benzylaminopurine with varying concentrations of coconut water and banana homogenate without sucrose. Results revealed that the treatment of 30% coconut water and 40 g/L banana homogenate resulted in the greatest proliferation of new shoots (3.00 ± 0.873 and 1.57 ± 0.297, respectively), whereas treatment of 40% coconut water resulted in the greatest shoot elongation of 0.239 ± 0.026 cm. The current study suggested the incorporation of coconut water and banana homogenate as potential substitutes for carbon sources and growth stimulants in the regeneration of Meyer lemon.

Growth response of sweet kaffir lime (Citrus hystrix) seeds due to kinetin and coconut water application using tissue culture technique

Sweet kaffir lime is one of Aceh’s local fruit crops, which is rarely found anymore. The problem of sweet kaffir lime cultivation is the difficulty of generative and vegetative plant propagation. The general objective of this research is to preserve the Acehnese sweet kaffir lime from extinction. One way that can be done is through plant propagation in tissue culture. The experiment was conducted in the Plant Tissue Culture laboratory of the Faculty of Agriculture, Universitas Malikussaleh. This research used a two-factor, completely randomized design, and ten replications. Two factors were tried: the concentration of kinetin (0, 1, and 2 mg/L) and the concentration of young coconut water (0, 15, 30 %). The results showed that increasing kinetin concentration could reduce shoot length, number of leaves, root length and slow the root emergence time of sweet kaffir lime in vitro. The best treatment was the 0 mg/L kinetin (without kinetin). Increasing coconut water concentration could increase shoot length, number of leaves, and root length of sweet kaffir lime in vitro. The best treatment was 30% coconut water concentration.

Occurrence of Heavy Metal in Green Coconut Water and Surface Seawater of the Sea Beach Areas of Bangladesh

Background: Green coconut water is a most popular functional drink in all tropical countries. The presence of toxic heavy metals in surface seawater is a major problem globally, with a large share in developing countries like Bangladesh. The objective of the study was to assess the concentration of heavy metals like Pb, Cr, Ni, Cu, and Cd in green coconut water and surface seawater around sea beach areas of Bangladesh. Methods: Green coconut water and surface seawater samples were collected from six sea beach areas and transferred to the laboratory. Then, they were allowed for digestion, and finally, the concentration of heavy metal was assessed by the atomic absorption spectrophotometer (AAS). Results: In green coconut water, the concentrations of heavy metal exist bellowing the safe limit with the following order: Pb &gt; Cr &gt; Cu &gt; Ni &gt; Cd. But, Pb, and Cr were prevalent in surface seawater and found to decrease in a sequence of Pb &gt; Cr &gt; Ni &gt; Cd, Cu. Besides, Human health risks associated with the intake of these metals were evaluated in terms of metal pollution index (MPI), daily intake of metals (DIM), health risk index (HRI), and hazard index (HI). However, most of the index values were found to be bellowing the reference value. Conclusion: The concentration of green coconut water is almost proportional to surface seawater in sea beach areas. The high prevalence of Pb and Cr in surface seawater is alarming, and this impact is already seen in green coconut water. So, it is necessary to take action on seawater pollution in Bangladesh.

Conservation of Gayo’s Endemic Orchid (Paphiopedilum primulinum) Through In Vitro Seed Germination and Development with Coconut Water

Paphiopedilum primulinum is one of endemic orchids in Gayo, Aceh, Indonesia. This terrestrial orchid is registered as endangered species due to IUCN. The low availability of this orchids in nature is due to habitat lost, over-collection for commercial purpose, and low seed germination rate which affected by unidentified factors, yet the study of propagating this orchid is rarely discussed. This experimental research is aim to see the effect of coconut water (CW) concentration to P. primulinum seed germination and development through in vitro culture. The sample of the study used the seed of P. primulinum which age 14 weeks after pollination. The research use ½ Murashige and Skoog media (MS) with four different coconut water (CW) concentration (0,10%, 20%, 30%) and incubated for 90 days. The seed germination rate is calculated due to the percentage of its total germinate seed and seed development indicator divided into 5 stages (0-5). The result shows that the seed of Paphiopedilum primulinum germinate best at 30% CW concentration and only in this concentration the seedling stage reach stage 5 (4.8%). This result implies that 30% CW concentration in ½ MS medium is the best medium to germinate this seed.

Regeneration of Cattleya Amazing Thailand in ½ MS Medium with The Addition of Several Concentration of PGR and Coconut Water

One of the Cattleya species with aesthetic and commercial value is Cattleya Amazing Thailand. An efficient protocol with a high rate of plantlet regeneration is through in vitro culture. Plantlet regeneration is affected by the addition of a Plant Growth Regulator (PGR). Auxin and cytokinin (2,4-D and BAP) combined with PGR from organic material such as coconut water can increase the growth of leave and roots in plantlet regeneration. The research aims to determine the best PGRs combination in regenerating the Cattleya Amazing Thailand orchid in vitro. The research used the Completely Randomized Design (CRD) experimental method for five treatments. The parameter are survival rate, number of leave, and number of roots. Survival rate was analyzed descriptively and both of number of leave and roots were analyzed statistically. All treatments can form 100% plantlets on survival rate parameter. The treatment of 0.1 mg/L 2,4-D + 1 mg/L BAP gave significantly different results in the number of leaf (9.0) and was not significantly different in the number of root. 0.1 mg/L 2,4-D + 1 mg/L BAP is the best combination for in vitro regeneration of the Cattleya Amazing Thailand orchid.

Effect of coconut water on in vitro propagation of Hylocereus costaricensus and acclimatization

Red-purple dragon fruit, Hylocereus costaricensis (Web.) Britton & Rose belongs to Cactaceae family. Due to its high nutraceutical ingredients has attracted a lot of attention to alternative propagate this species through in vitro culture technique. This research was aimed to analysed the effects of different concentrations of coconut water on the growth of H. costaricensis stem explants in Murashige and Skoog (MS) and Vacin and Went (VW) media. Then, combination of soil and coconut coir or coconut husk were used as planting media during acclimatization was investigated. Findings showed that in MS and VW supplemented with 5% coconut water formed 100% of shoot and root. VW media supplemented with 5% of coconut water yielded the highest average number of shoots (6.28 ± 0.48) and the longest shoot (3.85 ± 0.23 cm). The longest root (7.00 ± 0.66 cm) was observed on MS media supplemented with 5% coconut water whereas VW media supplemented with 5% coconut water produced the highest average number of roots (2.40 ± 0.19). Addition of coconut water can stimulate better shoot and root formation as it contains naturally plant growth regulators. During acclimatization, combination of soil and coconut coir showed the highest percentage (100%) of survival rate.

The Application of Table Sugar and Coconut Water to Enhance the Growth of Post-Acclimatized Moon Orchids (Phalaenopsis Hybrid)

Research on "The effect of giving granulated sugar and coconut water to increase the growth of moon orchids (Phalaenopsis hybrids)" was carried out from October 2022 to December 2022 at Sinoman Tempel, Sidorejo, Salatiga at an altitude of 500 meters above sea level. This research aims to determine the effect of the concentration of coconut water and granulated sugar on the growth of moon orchids (Phalaenopsis hybrids). The research method used was factorial with a Completely Randomized Design (CRD) consisting of two factors and including three replications. The data from this research were analyzed using the 5% BNJ test. The parameters observed included the number of Leaves at Last Time, Leaf Width, Leaf Length, Number of New Leaves, Root Length, and Live Percentage. There are two research factors, namely coconut water concentration treatment (A) and granulated sugar (B). The results of this study show that 1) Giving coconut water concentrate affects the number of leaves, leaf width, root length and live percentage; 2) Giving granulated sugar treatment has a significant effect on root length and live percentage; 3) The interaction between coconut water concentrate and granulated sugar has an effect on root length, number of recent leaves, and leaf width. 4) Providing the best treatment in the treatment of 75 ml/L coconut water concentrate and 30 gr granulated sugar, which has an effect on the parameters of root length, number of leaves, and survival percentage.

Effects of Different Concentrations of Coconut Water Extended with Glycerol and Ringers Solution Extenders on Cockerel Semen Preservation

The study was conducted at the Poultry Unit of the Teaching and Research Farm, Department of Animal Science, Federal University of Kashere, Gombe State. Nigeria, Kashere is located at an elevation of 431metre above the sea level. Its coordinates lies between latitude 9°46''O''N and longitude 100o57'0'' E, Altitude 349m (2006, census). The aim of the study was to determine the effects of cryptoprotectants on cockerel semen preservation. A total of twelve (12) indigenous cockerels with ages ranging from 12 to 15 months, with an unknown previous management background from local markets in Kashere were used. The cockerels were managed intensively and put into pens as experimental units. Cockerels were fed daily with growers mash feed and served water ad libitum; the birds were trained once a day in the morning by abdominal massage for two weeks. The cockerels were randomly allotted into three treatments. Each treatment had 3 replicates. The indigenous cockerels were trained once a day and semen was collected between 10:00am and 11:00am. The cockerels were allowed a period of two week for adaptation, Semen were collected by abdominal massage method described by Burrows and Quinn (1937). The semen concentration were measured using haemocytometer with the direct cell count method. The coconut water was collected from a freshly harvested coconut plant. Five milliliters of the coconut water was placed into different sampling bottles and diluted with different percentages of glycerol. The data on the percentage of sperm progressive motility, live/dead sperm, morphological normal intact sperm, total abnormal head, mid-piece/neck, tail and overall abnormalities were subjected to a one way analysis of variance using statistical Package for Social Sciences (SPSS version 23, 2015). coconut water extended with ringer solution at 2% glycerol at 0-7% concentration showed no significance difference (P&lt;0.05). In this study head sperm was significantly high (P&lt;0.05) at 2%, 3%, 5% and ..

Growth Response of Boots Resulting from Grafting of Rambutan Plants (Nephelium Lappaceum L.) on Various Concentrations of Young Coconut Water

This research aimed to determine the response of giving young coconut water to the growth of shoots resulting from grafting of rambutan plants. This research was carried out in Taruncue Hamlet, Alenangka Village, South Sinjai District, Sinjai Regency. This research was carried out using a Randomized Block Design (RAK) consisting of 4 treatments with 3 replications, so there were 12 experimental units. Each unit consists of 6 plants, so the total number of experimental plants is 72 experimental plants. The treatment consisted of: A0: Control, A1: 75 ml of young coconut water/liter of water, A2: 100 ml of young coconut water/liter of water, A3: 125 ml of young coconut water/liter of water. The results of the research showed that in the treatment of various concentrations of young coconut water that had the best influence on the growth of buds resulting from grafting of rambutan plants, there was a concentration of young coconut water of 100 ml of young coconut water/liter of water (A2) and had a very significant effect on the parameters of shoot length ( 5.67 cm), number of leaves (10.72 pieces) and a significant effect on shoot diameter (1.93 mm).

Growth of leaf cutting of malay apple (Syzygium malaccense (L.) Merr &amp; Perry) with coconut water treatmen

Malay apple (Syzygium malaccense (L.) Merr &amp; Perry) is a fruit plant in the Myrtaceae family, which has high economic value and nutritional content. The aim of this study was to determine the growth response of guava leaf cuttings to treatment with coconut water concentrations. The research design used was a randomized block design with 5 treatment levels and 5 replications. The treatment consisted of 5 levels namely control, water immersion, 25% coconut water, 50% coconut water, and 75% coconut water. Coconut water treatment was given to cuttings by soaking for 2 hours. Furthermore, leaf cuttings are planted in polybags that have been doused with water and then given a plastic cover. Parameters observed included the percentage of live cuttings, the percentage of formed roots, the percentage of shoots formed, the percentage of petiole damage, the percentage of petiole swelling and the percentage of petiole callus formation, the percentage of damaged leaves, and the symptoms of leaf damage. The results showed that the control treatment, water immersion and coconut water treatment showed the percentage of live cuttings was 100%, the leaf cuttings had not yet formed roots and shoots. Coconut water treatment reduced the percentage of petiole and leaf blade damage, increased the percentage of petiole swelling and increased callus formation, but was not able to stimulate root and shoot formation. Treatment of 75% coconut water showed the lowest percentage of damage to leaf cuttings with the highest percentage of swelling and callus formation.

Optimization of Mustard Pakcoy Growth: The Influence of Media Composition and Coconut Water Concentration

This research aimed to investigate the effect of different media compositions and varying coconut water concentrations on the growth and yield of mustard pakcoy (Brassica rapa var. parachinensis). The study was conducted in Modong Village, Sidoarjo Regency, with further laboratory analysis at the Faculty of Science and Technology, Universitas Muhammadiyah Sidoarjo. The experimental design followed a Randomized Block Design (RAK) in a factorial arrangement with two primary factors (media composition and coconut water concentration) and four replications. While no significant interaction was observed between the media composition and the coconut water concentration, independent effects of each factor were notable. The media composition significantly influenced the leaf count at 7, 28, and 35 days after planting (DAP) and the wet weight at 35 DAP. The optimum media composition included 2 kg of topsoil, 90 g of goat manure, and 45 g of rice husk charcoal per polybag, which resulted in the highest leaf count and wet weight. Additionally, coconut water concentration significantly affected the stem diameter at 35 DAP, with a concentration of 100 ml per liter of water yielding the maximum stem diameter. The findings imply potential growth optimization strategies for mustard pakcoy, enhancing yields via targeted alterations in growing media and the use of coconut water, which could be of international interest to agronomists, horticultural scientists, and sustainable agriculture practitioners.

Study on the effect of growth regulators and the concentration of coconut water on the growth and yield of garlic shallots (Allium ascalonicum L.)

An investigation on the influence of Atonic Growth Regulatory Substances and coconut water immersion on the growth and yield of shallots was conducted from March 3 to May 9 in 2023, in Tawangrejo Village, Ngrambe, Ngawi. The objective of this study is to analyze the impact of different doses of ZPT Atonik and coconut water on the growth and yield of shallots (Allium ascalonicum L). The experiment was designed using a Completely Randomized Block Design (RAKL), which included two factorials. The first factorial was the level of coconut water immersion concentration (K), consisting of four levels, namely K0 = 0%, K1 = 80%, K2 = 90%, and K3 = 100%. The second factor in this study is Atonik (A), which consists of four levels: A1 = 0 ml/l, A1 = 1.5 ml/l, A2 = 3.0 ml/l, and A3 = 4.5 ml/l. Each experiment in this study will be repeated three times and comprise four treatment levels, resulting in a total of 16 treatment combinations. We analyzed the data using Analysis of Variety followed by the Honest Significant Difference Test (BNJ) at the 5% level. The study results revealed that the Atonik treatment and coconut water immersion did not have a significant effect on the observed parameters of the number of leaves (40.30), number of tubers (17.87), tuber diameter (2.45 cm), fresh weight (130.63 g), and dry weight (109.22 g), but had a significant effect on the observed parameter of plant height (41.33 cm).

PLANT GROWTH OF MALONAN 1 PEPPER (Piper nigrum L.) CUTTINGS ON DIFFERENT PLANTING MEDIA COMPOSITION AND COCONUT WATER DOSAGE

The purpose of this study was to determine the effect of different planting medium compositions and concentrations of coconut water on the growth of pepper cuttings. This research was carried out for 3 months, starting from January to April 2020. This research site is in the experimental garden of the Faculty of Agriculture, Widya Gama University, Mahakam Samarinda.&#x0D; This research method used a randomized block design with 3 x 4 factorial experiments consisting of 3 replications. The first factor is the planting medium (M) which consists of 3 levels, namely: M0 Control (4 kg), M1 Topsoil + Goat Manure (3: 1) and M2 Topsoil + Goat Manure Fertilizer + Husk Charcoal (2: 1: 1) The second factor is Coconut Water which consists of 4 levels, namely: K0 (control), K1 (150 ml / 500 ml water), K2 (250 ml / 500 ml water) K3 (350 ml / 500 ml water).&#x0D; The variables analyzed were the number of shoots, plant height, and root length. The results of this study indicate that the best planting medium is found in treatment M2 = Topsoil (2 Kg) + goat manure (1 kg) + rice husk (200 g) and the best concentration of coconut water is found in treatment K3 = (350 ml / 500 ml of water) has the best effect on the growth of the pepper (Piper nigrum L.) Malonan 1&#x0D; &#x0D; Keywords: Malonan 1, Growing Media, Coconut Water

Effect of Coconut Water and GA3 Concentrations on in vitro Clonal Propagation of Potato Cultivars from Nepal

Nodal propagation plays a crucial role in mass multiplication of potato plants. Growth regulators and media selection have an impact on the efficacy and quality of propagation. Both coconut water, a naturally occurring organic source of growth-promoting compounds, and the synthetic growth regulator GA3 (gibberellic acid-3), have the ability to accelerate plant growth. The purpose of this study was to evaluate the effects of growth regulators and media, specifically GA3 and coconut water (CW) in agar-based media (ABM) and Clarigel-based media (CBM) which is a Gellan gum-based media, on potato nodal propagation. In contrast to the control group, GA3 in ABM did not produce definitive results, however GA3 in CBM showed a considerable level of efficacy. For the Janak Dev variety, CBM surpassed ABM in terms of root length, root hairs, leaf size, and dry biomass, whereas ABM demonstrated superior root length for the cardinal variety. In comparison to the GA3 alone at concentration ranges from 0 to 2 mg/L, adding CW at 200 mL/L to CBM or combining GA3 (0.25 mg/L) and CW (10 mL/L) substantially enhanced features including shoot length, leaf size, and root growth for both kinds. Acclimatized plantlets had a survival efficiency of 85% to 95%, with CBM supplemented with GA3 showing the highest survival rate and CBM supplemented with CW coming in second. These results highlight the significance of growth regulator and media choice in enhancing potato nodal propagation for improved plant quality and multiplication.

P-210 Utilization of coconut water as an additive to improve the human sperm quality following freezing-thawing Process

Abstract Study question Is the supplementation of the cryosolution with coconut water (CW) improve the motility and DNA integrity of spermatozoa after cryopreservation? Summary answer Coconut water as an additive to the cryosolution found to decrease the sperm DNA fragmentation and improve the sperm motility post-thawing. What is known already Despite the benefits of cryopreservation, it has adverse effects on sperm cell in different scenarios. Decrease in motility, viability and DNA fragmentation are the most common effects of cryopreservation on sperm function. The freezing/thawing shock produces physical and chemical stresses on the spermatozoa that could change the composition of the lipids in their plasma membrane resulting in increased production of reactive oxygen species (ROS) and increases the oxidative stress. Thus, supplementing the cryosolution with antioxidants may improve the recovery rate of cryopreserved spermatozoa by reducing the oxidative damage. Coconut water is characterized by its high contents of proven antioxidant properties. Study design, size, duration The study was conducted on 70 semen samples from patients attended the fertility center in Al-Sadder medical city, Najaf, Iraq, for routine semen analysis over a period of ten months from March to December 2022. All the patients gave their consent for participation in this study. The samples had normal semen parameters according to World Health Organization (WHO) 2010 standard criteria. Participants/materials, setting, methods Sperm motility and DNA fragmentation index (SDF) test were done for each sample, then, each semen sample divided to three parts: P1: cryopreserved with glycerol-containing cryosolution only (SpermFreezTM), P2: cryopreserved with glycerol-containing cryosolution and 5%coconut water (CW), and P3: cryopreserved with glycerol-containing cryosolution and 10% CW. After 1-month vapor-dependent cryopreservation, all samples were thawed and the sperm motility assed using computer-assisted sperm analyzer (CASA) and DFI was evaluated using Acridine orang stain method. Main results and the role of chance The percentage of total sperm motility and progressive motility in fresh samples were (39% and 35% respectively) and the level of sperm DNA fragmentation was (21%). The total and progressive sperm motility decreased significantly (P &amp;lt; 0.05) post-thawing, while the SDF increased. After cryopreservation the percentage of total and progressive sperm motility exhibited a significantly elevated levels in P2 (10.1% and 7.9% respectively) and P3 (14.6% and 11.3% respectively) than in P1 (6.8% and 4.2% respectively), however, the level of DFI significantly (P &amp;lt; 0.05) decreased in both P2 (27.4%) and in P3 (24.5%) than in P1 (30.1%). In comparison between the two concentrations of CW, P3 recorded a significant increase in the recovery of sperm total and progressive motility (14.6% and 11.3% respectively) than P2 (10.1% and 7.9% respectively). Furthermore, the level of DFI in P3 (24.5%) decreased significantly than its level in P2 (27.4%). Limitations, reasons for caution This study was to evaluate the efficiency of CW in the improvement of human sperm motility and DNA integrity post-thawing. Further studies required to evaluate the effect of using CW in sperm cryopreservation on the success rate of the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Wider implications of the findings In this study, we found that the additive of CW to the glycerol-containing cryosolution (SpermFreezTM) can improve the human sperm motility and DNA integrity post-cryopreservation. The best recovery rate of sperm motility and DNA integrity was with the supplementation of 10% of CW to the cryosolution. Trial registration number Not applicable

Respon Pertumbuhan Setek Batang Rosmarinus officinalis L. Terhadap Variasi Media Tanam Dan Konsentrasi Air Kelapa Sebagai Zat Pengatur Tumbuh Alami

Rosemary is one of the aromatic herb plants that is known to challenges in its seed production because the propagation of rosemary still relies on seed germination with low germination rates. To overcome this. This research aims to determine the growth response of rosemary stem cuttings to the application of Plant Growth Regulator (PGR) in the form of coconut water, variations in growing media, and their interaction. The study was conducted using a Completely Randomized Design with the variation of growing media and different concentrations of coconut water PGR solution. The experiment was conducted in a greenhouse, with a total of 15 treatment combinations repeated 3 times. The observed parameters included root length, number of roots, and number of shoots, with observations made every two weeks. The results showed that the combination of coconut water concentration and variation in growing media significantly interacted with the growth parameters, namely root length and number of roots. Additionally, individual factors such as the mixed soil and rice husk charcoal growing medium and 75% coconut water concentration separately had a significant effect on the number of sprouts at 42 and 56 days after planting.

Pengaruh Lama Penyimpanan Benih dan Konsentrasi Rendaman Air Kelapa terhadap Viabilitas Benih Padi (Oryza sativa L.)

Effect of Seed Storage Time and Coconut Water Soak Concentration on Viability of Rice Seed (Oryza sativa L.). This study aims to determine the effect of seed storage time and concentration of coconut water immersion on viability of rice seeds (Oryza sativa L.). This study used a completely randomized design (CRD) split plot. The main plot is the storage time of seeds consisting of 3 levels, namely storage time of 7 months (L1), 8 months (L2) and 9 months (L3). Sub-plots were the concentration of coconut water immersion consisting of 4 levels, namely immersion without coconut water (K0), 10% coconut water (K1), 20% coconut water (K2) and 30% coconut water (K3). Each treatment was repeated three times. This research was carried out at the Plant Breeding and Seed Technology Laboratory, Faculty of Agriculture, Udayana University from October 2021 to December 2021. The results showed a storage period of 7 months and a concentration of 10% coconut water (L1K1) which had the highest growth rate of 84.00% compared to with a storage period of 9 months and without soaking coconut water (L3K0) which has the lowest value of simultaneous growth of 52.00%. The storage period of 7 months gave the highest value for the variables of growth speed (23.96%/etmal), simultaneous growth (79.42%), germination (96.17%) and maximum growth potential (97.42%) compared to storage time. 8 and 9 months. The 10% coconut water concentration gave the highest value for the variable growth speed (23.65%/etmal) and maximum growth potential (96.44%) compared to no coconut water immersion. The concentration of 30% gave the highest value for the simultaneous growth variable (75.22%) compared to no coconut water immersion.

Effect of Coconut Water and Benzyl Adenine with Different Concentrations on Potato Tissue Culture (Solanum tuberosum L.) Var. Granola

This study aimed to determine the effect of giving coconut water and the right concentration of benzyl adenine in MS media for potato tissue culture of Granola variety. The study was carried out from November to January 2022, in the Plant Tissue Culture Laboratory of the Faculty of Agriculture, Pattimura University, Ambon. This study used a 2-factor completely randomized design (CRD); the first factor was the concentration of coconut water 0 with 100 ml/l, and the second factor was the concentration of BA consisting of 0.1,2,3,4 mg/l. The study was conducted with 7 replicates and each replication consisted of 5 explants in each culture bottle, so that there were 350 cultures as the unit of observation. The variables observed in this study included the number of shoots, the number of internodes, the number of branches, and the number of roots per plantlet. The results showed that the concentration of coconut water 0 and 100 ml/l had an effect on the number of internodes and the number of roots at 4 weeks after planting. Benzyl adenine at 0, 1, 2, 3 and 4 mg/l had an effect on the variable number of shoots at 8 WAP and the number of internodes at 2 - 8 WAP; which also applies to the number of roots. Whereas, the interaction between both factors affected the number of internodes at 4, 6, and 8 weeks, which also applies to the number of roots.