Concentrations Of 25-hydroxyvitamin Research Articles

Seasonal dynamics of 25-hydroxyvitamin D and biogenic amines in the blood of apparently healthy women living in the city of Arkhangelsk

The aim of the study is to assess the seasonal supply of vitamin D and biogenic amines to practically healthy women of working age living in Arkhangelsk. Material and methods. The study sample included 20 practically healthy women aged 22–44 years, permanently residing in Arkhangelsk. The study was conducted over 4 seasons: during the period of increasing (March), maximum (June), decreasing (September) and minimum daylight hours (December). The concentration of 25-hydroxyvitamin D, serotonin, dopamine, adrenaline and noradrenaline in the blood were determined. Results. The annual dynamics of 25-hydroxyvitamin D level in the blood was established with its minimum content in the winter (December), followed by an increase in the vitamin concentration in the spring, the highest level during the period of maximum daylight hours (June) and a further decrease in the fall (September). During the period of minimum day length, the smallest proportion of people (15 %) with optimal 25-hydroxyvitamin D concentration is observed, while more than half of women have insufficient level, and a quarter have vitamin D deficiency. Serotonin level decreased from spring to winter. The peak of dopamine concentration was recorded in the summer; the minimum content was recorded in the autumn. The minimum concentration of adrenaline and noradrenaline is noted in the spring; the maximum levels are in the winter. Conclusions. The most critical season for 25-hydroxyvitamin D content in women blood is the minimum duration of daylight hours’ period (December), while the more favorable season can be attributed to the maximum duration period (June) and a decrease in the duration of daylight hours (September). The annual 25-hydroxyvitamin D dynamics and serotonin levels have similar features, and seasonal changes in the adrenaline and noradrenaline concentration are in the opposite direction.

Insufficient serum levels of 25-hydroxyvitamin d are associated with dyslipidemia in the elderly

Objective: To identify possible associations between serum levels of 25-hydroxyvitamin D and dyslipidemia in the elderly. Method: A cross-sectional study. For sample consisted of 80 elderly people monitored at a Geriatric Outpatient Clinic of the University Hospital in Alagoas, Brazil. In this study sociodemographic variables, lifestyle, health conditions, anthropometrics, body composition and biochemical tests were collected (serum lipid profile, 25-hydroxyvitamin D, us-RCP, PTH and calcium). Results: It was observed that the concentrations of 25-hydroxyvitamin D correlated negatively with the serum concentrations of total cholesterol and LDL-c. There was no association between HDL-c, triglyceride concentrations and the variables studied (p>0.050). 25-hydroxyvitamin D was associated with the diagnosis of dyslipidemia (p=0,032). Discussion: Vitamin D was shown to be a protective factor for the development of dyslipidemia in the elderly studied and the insufficiency in serum level of vitamin D seemed to exert an influence on lipid metabolism, making the elderly even more vulnerable cardiovascular diseases.

The problem of increased vitamin D3 level in a group of patients hospitalized in a geriatrics clinic

Taking into account the multi-directional beneficial effects of vitamin D3 and its widespread deficiency, regular supplementation is recommended. However, more and more attention is being paid to the risk of overdose with supplemented vitamin D3 and the associated serious health consequences. The concentration of 25-hydroxyvitamin D (25(OH)D) is a routine test recommended upon admission to the Geriatrics Clinic of Wroclaw Medical University. The aim of the study was to analyze the results from January 2018 to June 2023. Additionally, information on the reported symptoms, gender and age of people with an increased level of vitamin D3 was collected. Analyzing a group of 1400 patients, it was noted that within 5 years, vitamin D3 concentrations exceeding the recommended level were recorded in 7 patients, including 3 with toxic levels. All abnormal results occurred in women. The most frequently reported symptoms included general weakness, lower limbs and joint pain, sleep disorders, low mood. People with toxic concentrations reported dizziness. In seniors there is a gradual increase in vitamin D concentration and its deficiency is less common. Higher concentrations were recorded in the group of older seniors, and concentrations considered toxic occur in the population >74 years of age. Supplements and drugs with vitamin D are most often used without consulting a doctor, without determining the appropriate dose, or without assessing the concentration of 25(OH)D in the serum. To prevent vitamin D deficiency in seniors, doses >4000 IU daily are not recommended. It is advisable to check all medications and supplements taken at each doctor's visit in terms of duplicating treatment with vitamin D. It is advisable to assess the status of vitamin D supply the concentration of 25(OH)D in order to select the appropriate dose. Assessment of 1,25-dihydroxyvitamin D concentration is recommended in cases of vitamin D toxicity. Med Pr Work Health Saf. 2024;75(3).

Optimisation of vitamin D status in global populations.

Vitamin D is important for musculoskeletal health. Concentrations of 25-hydroxyvitamin D, the most commonly measured metabolite, vary markedly around the world and are influenced by many factors including sun exposure, skin pigmentation, covering, season and supplement use. Whilst overt vitamin D deficiency with biochemical consequences presents an increased risk of severe sequelae such as rickets, osteomalacia or cardiomyopathy and usually warrants prompt replacement treatment, the role of vitamin D supplementation in the population presents a different set of considerations. Here the issue is to keep, on average, the population at a level whereby the risk of adverse health outcomes in the population is minimised. This position paper, which complements recently published work from the European Society for Clinical and Economic Aspects of Osteoporosis, Osteoarthritis and Musculoskeletal Diseases, addresses key considerations regarding vitamin D assessment and intervention from the population perspective. This position paper, on behalf of the International Osteoporosis Foundation Vitamin D Working Group, summarises the burden and possible amelioration of vitamin D deficiency in global populations. It addresses key issues including screening, supplementation and food fortification.

Abstract 6130: Association of vitamin D-related genetic variants and prostate cancer risk in Black men

Abstract Background: What we know of the relationship between vitamin D status and prostate cancer has largely been characterized among men of European ancestry, hereafter referred to as “White”. Research among White men indicate positive associations between circulating vitamin D status, as measured by concentrations of 25-hydroxyvitamin D (25(OH)D), with prostate cancer risk, and that genetic scores based on genome-wide associated single nucleotide polymorphisms (SNPs) reflecting high vs. low 25(OHD) is positively associated with aggressive disease. Limited research exists elucidating this relationship among men of African ancestry, hereafter referred to as “Black”. This is despite the fact that Black populations experience chronically lower circulating 25(OH)D, and ancestry-specific differences in genetic variants in some vitamin D-related genes. Given the paucity of research in this area, we examined the role of genetic variants in six commonly studied genes in the vitamin D pathway involved in synthesis (CYP27A1 and CYP27B1), transport (GC), activity (VDR and RXR), and metabolism (CYP24A1) in relation to prostate cancer among Black men. Methods: We assessed a total of 78 candidate SNPs in six vitamin D pathway genes that were either previously associated with circulating 25(OHD) and/or associated with prostate cancer risk in studies of White men. We compared vitamin D gene SNP associations with prostate cancer in Black and White men using summary statistics from a large GWAS of over 10,000 prostate cancer cases and 10,000 controls among Black men and over 85,000 cases and 91,000 controls among White men. A statistical significance threshold of 0.00064 (0.05/78 candidate SNPs) was used to adjust for multiple comparisons. Results: None of the examined SNPs were significantly associated with prostate cancer risk among Black men after adjustment for multiple comparisons. However, four SNPs were nominally significant in Black men, including two in RXR [rs41400444 OR=1.09, 95% CI: 1.01-1.17, P = 0.024 and rs10881574 OR = 0.93, 0.867-0.999, P = 0.04556] and two in VDR [rs2853563 OR = 1.07, 1.012-1.13, P = 0.0173 and rs1156882 OR = 1.06, 1.00-1.12, P = 0.0495]. The latter SNP rs1156882 was also positively associated with prostate cancer risk in White men (OR = 1.035, 1.02-1.06, P = 0.00024), along with VDR SNP rs4516035 (OR = 1.027, 1.012-1.042, P = 0.00055), both after correction for multiple comparisons. Conclusions: We observed non-significant, though suggestive, associations between genetic variants in vitamin D pathway genes previously associated with prostate cancer or vitamin D levels in White populations and prostate cancer risk in Black men. Future research exploring the potential role of vitamin D pathway genes and prostate cancer in Black men – a population at high risk both for low circulating vitamin D and prostate cancer - will need to identify vitamin D-related genetic variants specific to carcinogenesis in Black men. Citation Format: Tracy M. Layne, Joseph H. Rothstein, Xiaoyu Song, Weiva Sieh, Robert J. Klein. Association of vitamin D-related genetic variants and prostate cancer risk in Black men [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 6130.

Vitamin D status & associations with inflammation in older adults.

Research studies have observed associations of vitamin D with inflammation but data in representative older adult studies is lacking. We aimed to investigate the association of C-reactive protein (CRP) with vitamin D status in a representative sample of the older Irish population. The concentrations of 25-hydroxyvitamin D (25(OH)D) and CRP was measured in 5,381 community dwelling Irish adults aged ≥50 years from the Irish Longitudinal Study on Ageing (TILDA). Demographic, health and lifestyle variables were assessed by questionnaire and categorical proportions of CRP were generated by vitamin D status and age. Multi-nominal logistic regression was used to investigate the association of 25(OH)D and CRP status. The prevalence (mean; 95% confidence interval (95% CI)) of normal CRP status (0-5 mg/dL) was 83.9% (82.6-85.0%), elevated status (5-10 mg/dL) 11.0% (9.9-12.0%) and high status (>10 mg/dL) was 5.1% (4.5-5.8%). Mean (95% CI) CRP concentrations were lower in those with normal vs. deficient 25(OH)D status (2.02 mg/dL (1.95-2.08) vs. 2.60 mg/dL (2.41-2.82); p<0.0001). In a logistic regression analysis, those with insufficient or sufficient 25(OH)D status were less likely to have a high CRP status compared to those with deficient 25(OH)D status (insufficient: coefficient (CE) -0.732, 95% CI -1.12-0.33, p<0.0001; sufficient: CE -0.599, 95% CI -0.95-0.24, p = 0.001). In conclusion older adults with deficient vitamin D status had higher levels of inflammation as measured by CRP. Given that inflammation is an important pathological driver of chronic diseases of ageing, and that emerging evidence suggests that vitamin D therapy can reduce inflammation in some disease settings, optimising vitamin D status could represent an effective low risk/low-cost pathway to modulate inflammation in community dwelling older adults.

Abstract 1909: Pre-diagnostic vitamin D in association with colorectal cancer- and all-cause mortality: a prospective study among a non-Hispanic Black cohort

Abstract Purpose: Evidence from RCTs suggests vitamin D supplementation may improve colorectal cancer (CRC) outcomes after diagnosis. However, most trials did not include large numbers of Black patients, who typically have lower vitamin D levels. We evaluated associations between vitamin D with all-cause and CRC-specific mortality after CRC diagnosis. Methods: Data arose from 220 participants of the Southern Community Cohort Study diagnosed with incident CRC. Eligible patients were ages 40-79 at enrollment, non-Hispanic Black, English-speaking, and provided a blood sample at enrollment for biomarker measurement. Patients provided information on lifestyle factors, demographics, and personal medical history, primarily via in-person interviews. Concentration of 25-hydroxyvitamin D was measured from blood samples taken at enrollment and were categorized by the Institute of Medicine’s categories of deficient (&amp;lt;12ng/mL), inadequate (12-19.9ng/mL), or adequate (≥20ng/mL). Vital status was obtained through linkages with the National Death Index through December 31, 2020. Cox proportional hazards were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) for associations between 25-hydroxyvitamin D concentration and mortality. Statistical models adjusted for the following covariates: age at diagnosis, race, sex, cancer stage, education, income, alcohol intake, smoking history, screening history, obesity, insurance coverage, and physical activity. Results: Circulating 25-hydroxyvitamin D concentration was inversely associated with CRC-specific and all-cause mortality. Specifically, patients with pre-diagnosis 25-hydroxyvitamin D concentration categorized as adequate had reduced all-cause mortality in comparison to participants with deficient vitamin D concentration with a HR of 0.58 (95%CI: 0.35, 0.97). Stronger associations between 25-hydroxyvitamin D concentration and all-cause mortality were observed among participants where blood draw occurred three-five years before diagnosis. Associations were strongest among current smokers where the HR for each per-standard deviation increase of 25-hydroxyvitamin D concentration with all-cause mortality was 0.58 (95%CI: 0.36,0.92), and among obese participants the HR for a per-standard deviation increase of 25-hydroxyvitamin D with all-cause mortality was 0.45 (95%CI: 0.27,0.75). An inverse association was also observed with CRC-specific mortality, although the association was attenuated (HR for per-standard deviation increase of 25-hydroxyvitamin D=0.83; 95%CI: 0.63,1.08). Conclusions: Our findings suggest that pre-diagnosis vitamin D concentration is inversely associated with mortality after a CRC diagnosis among Black individuals. Correcting vitamin D deficiency may be warranted among Black CRC patients, particularly for those who smoke or are obese. Citation Format: Shaneda Warren Andersen, Thomas Lawler, Nikhil K. Khankari, Xiao-Ou Shu, Mark D. Steinwandel, Qiuyin Cai, Peter W. Peter W Jurutka, Wei Zheng. Pre-diagnostic vitamin D in association with colorectal cancer- and all-cause mortality: a prospective study among a non-Hispanic Black cohort [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 1909.

PSAT164 Incidence and Severity of Hypocalcemia in Association with Antiresorptive Therapy in Cancer Patients with Bone Metastasis: An Integrated Health Network Experience

Abstract Introduction Antiresorptive therapy (ArT) has been known to decrease the risk of skeletal-related events (SRE), such as bone pain and pathological fractures, in cancer patients with bone metastasis (BM). While beneficial, ArT may cause adverse effects in this population including hypocalcemia, which can be severe and life-threatening. We examined the incidence, severity, and risk factors for hypocalcemia associated with the use of ArT to treat BM in cancer patients. Methods We conducted a retrospective review of the electronic medical records of adult patients with advanced cancer complicated by BM who were treated at our institution between January 2016 and November 2020. Our primary aim was to compare the incidence and severity of hypocalcemia with the use of IV Zoledronic acid (ZA), a bisphosphonate, vs SQ Denosumab (DM), a RANKL inhibitor, to treat BM in cancer patients.Our secondary aim was to compare risk factors associated with severe hypocalcemia (defined as albumin-corrected serum calcium &amp;lt; 7 mg/dL). Inclusion criteria were patients aged 19-99 years with a history of cancer with known BM, who received either ZA or DM. Results We reviewed 860 EMRs of patients treated within the specified period. 392 patients met inclusion criteria, among them, 297 (76%) and 95 (24%) received DM and ZA, respectively. There were no between-group differences in age, ethnicity, or weight. The median patient age was 71 years (range: 27–96). There were, however, between-group differences in gender (p=0.01) and cancer diagnoses (p=0.03). Females comprised 53.9% of the DM group vs. 40% of the ZA group. The top three cancer diagnoses were (DM vs ZA groups): breast (40.1% vs 26.3%), prostate (25.9% vs 40%), and lung cancer (17.8% vs 15.8%). Overall incidence of hypocalcemia was 48.8% (n=144) and 45.3% (n= 43) with the use of DM and ZA, respectively (p=NS). Severe hypocalcemia was more frequently observed with the use of DM vs ZA (6.6% vs 1.1%, p &amp;lt;0.05). Median (IQR) corrected serum calcium concentration following ArT (when trending calcium for the individual patient) was 8.4 (7.9-8.8) and 8.6 (8.2-8.8) mg/dL for DM a ZA groups, respectively (p=0.1). There were no between-group differences in risk factors for hypocalcemia, such as renal function (eGFR), concentrations of 25-hydroxy vitamin D, albumin, phosphorus, magnesium, or TSH. However, there was a between-group difference in the concentration of alkaline phosphatase (median 83 vs 101 IU/L for DM vs ZA, p= 0.02). There were no between-group differences in the type of BM (osteolytic vs osteoblastic, p=0.9) Conclusion: Severe hypocalcemia was significantly more common with the use of DM compared to ZA for the treatment of BM in cancer patients treated at our institution. Our study was limited by its retrospective design and the between-group differences in gender distribution and cancer diagnoses. Presentation: Saturday, June 11, 2022 1:00 p.m. - 3:00 p.m.

Influence of Nordic walking with poles with an integrated resistance shock absorber on carbohydrate and lipid metabolic indices and white blood cell subpopulations in postmenopausal women.

BackgroundRegular and individualised physical activities have been shown to prevent adverse changes associated with the aging process. The main purpose of this study was to evaluate changes in carbohydrate and lipid metabolism and white blood cell (WBC) subpopulations in postmenopausal women participating in Nordic walking (NW) training and to compare the use of poles with an integrated resistance shock absorber (RSA) with the use of classic poles.Materials & MethodsA total of 23 postmenopausal women participated in a 8-week programme of systematic physical activity between February and April. Before and after the training programme, somatic features and serum concentrations of 25-hydroxyvitamin D, glucose, and insulin, were assessed, as well as lipid profile and WBC count and its specific subpopulations.ResultsAnalysis of differences in somatic features and biochemical indices before and after training in the group of women who used RSA poles showed significant decreases in fat mass content (p < 0.05), insulin (p < 0.05), homeostatic model assessment of insulin resistance (p < 0.05), triglycerides (p < 0.05), total cholesterol (p < 0.05) and monocytes (p ≤ 0.01). In the group of women who used classic poles (NW), there was a significant decrease in WBC (p ≤ 0.01), lymphocytes (p < 0.05), monocytes (p ≤ 0.01) and granulocytes (p < 0.05).ConclusionIncreasing the training load through the use of RSA poles resulted in greater changes in carbohydrate and lipid metabolic indices compared to the use of classic NW poles. In turn, the more significant effect on WBC and its specific subpopulations count in the NW, compared to the RSA training programme, may indicate that specificity of training load is an important factor in modifying the immune system response.

Effect of hypovitaminosis D on the characteristics of chronic tension-type headache in women

There is a growing body of evidence linking hypovitaminosis D with the prevalence and severity of various pain conditions. The effect of vitamin D levels on chronic tension type headache (CTTH) is almost unexplored.Objective: to investigate the effect of serum vitamin D levels on the clinical characteristics of CTTH.Patients and methods. The study enrolled women with CTTH (n=161). Headache frequency (HF), headache duration (HD), headache intensity (HI), presence of chronic widespread pain (WP) and the number of its zones, severity of anxiety and depression, and sleep quality index were evaluated. The vitamin D level was assessed by the concentration of 25-hydroxyvitamin D [25(OH)D].Results and discussion. Low 25(ОН)D-level was associated with an increase in HF, HD and the prevalence of WP. For each of the listed parameters, the values in vitamin D deficiency were higher than in its insufficiency or normal ranges (p&lt;0,001). 25(OH)D-level was inversely correlated with HF (rS=-0,49), HD (rS=-0,51; p&lt;0,05). Vitamin D-deficiency increased the risk of WP: compared to its insufficiency relative risk (RR) was 2.12 (95% CI 1.39–3.21), to the normal ranges – 3.67 (95% CI 1.71–7.88). In case of insufficiency and normal level of vitamin D, the values for the listed parameters did not differ. Other characteristics did not depend on 25(OH)D-level.Conclusion. In women with CTTH hypovitaminosis D contributes to the greater disease severity. Vitamin D deficiency, which is strongly associated with an increase in pain characteristics, has great clinical significance.

Possibilities of colecalciferol therapy for tension type headache combined with hypovitaminosis D

Introduction. Modern studies indicate the therapeutic effect of vitamin D (VD) in chronic pain conditions, but there is no data on the use of VD in chronic tension type headache (CTTH) treatment.Objective: comparative evaluation of the effectiveness of various options for preventive treatment of CTTH: the use of colecalciferol, standard therapy of the disease and a combination of these methods.Materials and methods. 125 women with CTTH and hypovitaminosis D were treated in the study. The frequency, duration and intensity of headache (HF, HD and HI, respectively), HIT index, frequency of analgesics taking, pericranial muscle dysfunction were evaluated. The VD level was measured by the concentration of 25-hydroxyvitamin D [25(OH)D]. The patients received differentiated therapy for 16 weeks: group 1 – colecalciferol, group 2 – amitriptyline, group 3 – a combination of these medicines. Results. In group 1, HF, HD, HIT-index and the frequency of analgesics taking decreased, all p ≤ 0.001. In groups 2 and 3, the values of all CTTH-parameters decreased, all p &lt; 0.05. HF, HIT-index and the frequency of analgesics taking in group 3 decreased more than in group 2, all p &gt;&lt; 0.017. In women who had VD deficiency before treatment, the decrease in HF from the initial one was 36% in group 1, 55% in group 2, and 74% in group 3. In patients who had VD deficiency before treatment, the effectiveness of therapy in group 3 was higher than in group 2: 74% vs. 55%, respectively, p &gt;&lt; 0.001.Conclusions. Treatment of hypovitaminosis D in women with CTTH is accompanied by a decrease in HF, the effect of headache on the quality of life of patients and the frequency of taking analgesics. In CTTH and VD deficiency normalization of 25(OH)D level contributes to higher efficiency of standard therapy.

Роль ендогенних антимікробних пептидів у виникненні пневмонії в дітей раннього віку

Проведено комплексне обстеження 204 дітей віком від 2 місяців до 3 років із позалікарняною пневмонією. Установлено, що в дітей раннього віку, хворих на позалікарняну пневмонію, провідним етіологічним фактором є бактерії Streptococcus pneumoniae (36,8 %). Вміст ендогенних антимікробних пептидів визначено в сироватці крові 20 дітей раннього віку, хворих на позалікарняну пневмонію, та 17 дітей групи контролю. Доведено, що розвиток позалікарняної пневмонії в дітей раннього віку відбувається на фоні зниження вмісту в сироватці крові β1-дефензину та кателіцидину LL-37. Найбільш низькі значення LL-37 були встановлені в дітей, хворих на пневмонію, викликану Streptococcus pneumoniae. Проведений аналіз вмісту метаболітів вітаміну D у сироватці крові показав, що в дітей, хворих на позалікарняну пневмонію, концентрація 25-гідроксивітаміну D була в 1,4 раза нижчою порівняно зі здоровими дітьми (р &lt; 0,05). Установлений дефіцит метаболітів вітаміну D у дітей раннього віку, хворих на позалікарняну пневмонію, є значущим патогенетичним фактором дефіциту кателіцидину LL-37 у сироватці крові, що підтверджувалося зниженням у даній когорті хворих в 3,7 раза процентного вмісту LL-37 щодо метаболітів вітаміну D.

Корекція рівня забезпеченості вітаміном D у дітей раннього віку з повторними епізодами бронхообструктивного синдрому

Актуальність. Схильність до частих гострих респіраторних вірусних інфекцій (ГРВІ) і, зокрема, повторного перебігу бронхообструктивного синдрому (БОС) у дітей раннього віку залишається актуальним питанням педіатрії. Останнім часом серед факторів ризику повторних епізодів БОС особлива увага приділяється вивченню рівня забезпеченості вітаміном D (VD, 25-гідроксивітамін-D (25(ОН)D). Нещодавні дослідження демонструють зв’язок між низьким рівнем VD і підвищеною сприйнятливістю до вірусних інфекцій. Водночас питання щодо вивчення необхідності корекції статусу VD у дітей із повторним БОС є досі не вивченим. Мета. Вивчення рівня забезпеченості VD та ефективності корекції його порушень у дітей раннього віку з повторними епізодами БОС. Матеріали та методи. Обстежено 120 дітей віком від 6 місяців до 3 років із клінічним діагнозом «гострий обструктивний бронхіт». Пацієнти були розподілені на дві групи (1-ша група — 60 дітей з епізодичним БОС (менше 3 епізодів протягом року), 2-га група — 60 дітей із повторним БОС (3 і більше епізодів протягом року)). Групу контролю становили 30 клінічно здорових дітей віком від 6 місяців до 3 років. Усім хворим проводили оцінку анамнестичних даних і в тому числі тих, що впливають на рівень забезпеченості VD. Визначали концентрацію 25(OH)D у сироватці крові електрохемілюмінесцентним методом на аналізаторі Cobas e411 (серійний номер 1041-24, виробник — Roche Diagnostics GmbH, Німеччина). Методом простої рандомізації із групи з повторним БОС обрано 30 пацієнтів (2а група), яким призначали препарат вітаміну D3 у дозі 1000 МО щодня протягом року, за винятком літніх місяців. Тривалість катамнестичного спостереження — 12 місяців. Ефективність застосування вітаміну D3 оцінювали на підставі динаміки респіраторної захворюваності, частоти епізодів БОС, а також за динамікою показників сироваткового рівня 25(OH)D. При аналізі отриманих даних використовували непараметричні статистичні критерії. Різниця між показниками, що порівнювались, вважалась вірогідною при р &lt; 0,05. Результати. Більшість пацієнтів із повторним перебігом захворювання (56,6 %) мали 4 епізоди БОС за останні 12 місяців. Середній вміст 25(OH)D у сироватці крові в дітей 1-ї групи становив 33,0 (28,19; 41,97) нг/мл, 2-ї групи — 13,68 (7,96; 19,51) нг/мл (p &lt; 0,001). Аналіз анамнестичних показників, які впливають на рівень VD, виявив, що ймовірність недостатності або дефіциту VD у дітей раннього віку з БОС, які не отримують вітамін D, набагато вища, ніж у дітей, які приймають його щодня в дозі 500–1000 МО (OR = 0,047; 95% СІ 0,018–0,126; р &lt; 0,001). Виявлено зворотну залежність між рівнем VD як із частотою БОС (r = –0,27, р = 0,002), так і з віком обстежених пацієнтів (r = –0,33, р = 0,009). У дітей 2-ї групи середня частота епізодів ГРВІ за останній рік була вище, ніж у пацієнтів 1-ї групи (4,5 (3,0;5,5) і 3 (2,0;5,0) відповідно, р &lt; 0,001), при цьому виявлено, що середній рівень 25(OH)D у сироватці крові мав тенденцію до більш низьких показників за наявності 5 і більше епізодів ГРВІ на рік. Після прийому препарату вітаміну D3 у більшості пацієнтів відзначалось зменшення частоти БОС до 1–2 епізодів протягом року, а також зниження респіраторної захворюваності на 50 %. Ми виявили, що після застосування вітаміну D3 спостерігалось вірогідне зростання концентрації сироваткового рівня 25(OH)D порівняно з його рівнем, визначеним на початку дослідження (25,11 (12,14; 42,47) нг/мл і 13,93 (9,60; 20,5) нг/мл відповідно, p = 0,002 за Т-критерієм Вілкоксона). Висновки. Преважна більшість пацієнтів із повторним перебігом БОС мають дефіцит VD, що може розглядатись як предиктор повторних епізодів захворювання. На рівень забезпеченості VD найбільшою мірою впливає рівень його саплементації. Дітям раннього віку, які часто хворіють на ГРВІ, і в тому числі з повторним БОС, важливо оцінювати анамнестичні дані щодо застосування вітаміну D та при необхідності визначати рівень 25(OH)D у сироватці крові. Встановлено, що при зниженні сироваткової концентрації 25(OH)D кількість епізодів БОС і ГРВІ зростає. Також рівень VD має тенденцію до більш низьких показників при збільшенні віку пацієнтів. Щоденний прийом 1000 МО вітаміну D3 протягом 12 місяців сприяє зменшенню частоти ГРВІ і в тому числі епізодів БОС у дітей раннього віку.

Effects of Vitamin D2 and 25-Hydroxyvitamin D2 Supplementation on Plasma Vitamin D Epimeric Metabolites in Adult Cats.

Feline vitamin D status is based on dietary consumption but metabolism of this essential nutrient and the efficacy of supplementation forms are poorly described in cats. The aim of this study was to further elucidate the metabolites of vitamin D2 in cats and to compare the effectiveness of vitamin D2 and 25(OH)D2 for increasing feline vitamin D status. Eight adult male castrated domestic shorthair cats received vitamin D2 or 25(OH)D2 in a single crossover design. Vitamin D2 was dosed daily in a molar equivalent dosage to vitamin D3 ingested in the diet while 25(OH)D2 was provided at a daily dose of 20% molar equivalent intake of dietary vitamin D3 based on its expected higher potency. Plasma concentrations of 25-hydroxyvitamin D epimers were evaluated at baseline then every 2 weeks for a total of 10 weeks. Analysis of multiple vitamin D metabolite concentrations was completed at the end of each supplementation period, followed by a washout period preceding the second phase of the crossover trial. Results showed that supplementation with 25(OH)D2 more effectively and rapidly raised circulating 25(OH)D2 levels in cat plasma compared to vitamin D2. Formation of C-3 epimers of 25(OH)D3, 25(OH)D2, and 24,25R(OH)2D3, but not 24,25(OH)2D2, were observed in feline plasma. The abundant concentrations of epimeric forms of vitamin D metabolites found in circulation suggest that these metabolites should be considered during vitamin D analyses in cats. Further studies using 25(OH)D and vitamin D2 forms are needed to conclude safety and efficacy of these vitamers for supplementation in this species.

The significant role of dietary intake of vitamin D in non-menopausal women health

ABSTRACT Optimal vitamin D status is very important for reflecting not only bone but overall woman’s health. The aim of the study was to determine pharmacokinetic variability of 25-hydroxy vitamin D, to reveal and quantify the most significant factors that affect its variability in the population of healthy non-menopausal women using the population pharmacokinetic (PopPK) approach. The study population consisted of 74 healthy reproductive women aged from 35 to 50 years, without the use of any supplement. A population pharmacokinetics analysis was conducted using a nonlinear mixed-effects model software. A total of 35 factors were assessed: demographic, clinical, biochemical data and lifestyle factors. The average age and bodyweight of our participants were 40.11 ± 4.35 years 65.30 ± 6.80 kg, respectively. The observed mean serum concentration of 25-hydroxy vitamin D was 26.51 ± 13.49 ng/mL with a wide range of 6.97 to 59.89 ng/mL. Development final PopPK model of the clearance of 25-hydroxy vitamin D showed that only the average daily dose of vitamin D intake from food had a significant influence, with a magnitude of its effects of 0.00401. These results could help when individualizing vitamin D intake in the form of supplements, especially during the wintertime, in healthy reproductive women.

The Concentration Of 25-Hydroxy Vitamin D In Chronic Liver Disease And Its Correlation With Severity

Background: Chronic liver disease (CLD is a common disease all over the world and the major cliological factors for the causation of disease are HBV and HCV in this country and Alcoholic liver disease in the western world. With the availability of the modern treatment, the life expectancy is increased now a days. But the long term complications are now evident. One of the complications is hepatic osteodystrophy which is associated with deficiency of vitamin D. Vitamin D undergoes hepatic 25-hydroxylation, but as the hepatic parenchyma is jeopardized so the metabolic activation of this vitamin is impaired. Vitamin D deficiency is highly prevalent in CLD patients and vitamin D level is inversely related to the severity of the disease. Objective: To assess the concentration of 25-hydroxy vitamin D in chronic liver disease patient in different etiology and to study the relationship of level of 25(OH) D in different stages of the disease according to Child-Pugh classification. Methods: This cross sectional study was carried out in the Department of Gastroenterology, Bangabandhu Sheikh Mujib Medical University, Dhaka during the period of April 2015 to March 2016. Patient attending the Gastroenterology Department who fulfill the inclusion criteria with cirrhosis of liver were initially be enrolled for the study. Their clinical history, examination and initial investigation report were noted in the standard data sheet. After explaining the study objective, informed consent was taken. The diagnosis of liver cirrhosis was made by combination of clinical features, blood profile and transabdominal ultrasound. Endoscopy of the upper GIT was done to see the presence of oesophageal or gastric varices which is a sign of increase portal pressure. Transabdominal ultrasound demonstrated a shrunken liver with increase echogenicity, with or without splenomegally and presence or absence of ascites. Stages of liver disease were assessed by Child-Pugh scoring system. Level of 25(OH) D was measured from blood with the permission of the Department of Bio-chemistry. Data was collected using a structered data sheet. Results: Out of 85 patients, male were 61 (71.8%) and female were 24 (28.2%). Mean age was 53.0 ± 10.7 years within tin- range of 25-70 years. More than 90.0% patient* had abates and anorexia. Eighty percent patients had weight loss and 71.8% patients had Jaundice. More than 40.0% patients had abdominal pain and melaena. Sixty (77.9%) patients had history of blood transfusion and 73 (94.8%) patients had previous hospitalization, Most of the patients had anaemia (97.6%) and Splenomegaly (92.9%). More than 50.0% patients had jaundice (61.2%), Leukonychia (61.2%) and hone pain (52.9%). Mean s, vitamin 25(OH) D was 16.29 ± 7.96 in 69 HBV patients and 20.14 ± 9.76 in 16 HCV patients. In this study, 28.2% patients were in child Pugh A, 36.4% in child Pugh B and 32.9% in child Pugh C stages. Mean s. vitamin 25(OH) D were 27.12 ± 6.11, 15.97 ± 5.40 and 9.57 ± J.I5 in Child-pugh A, Child-pugh B and Child-pugh C stages respectively. Mean s. vitamin 25(OH) D was gradually decreased as the changes of stage from lower to higher. Conclusion: Vitamin D deficiency is highly prevalent in patients with CLD and inversely correlated with disease severity. In the case of chronic liver diseases, vitamin D seems to modulate the innate and adaptive immune system, which explains the association. In fact, clinical studies suggest that these parameters may improve with vitamin D supplementation. So, monitoring of S. 25(OH) D is reasonable in CLD patient.

Role of Vitamin D in Risk Reduction of COVID-19: A Narrative Review

The world is in the midst of the COVID-19 pandemic. In addition to quarantine, public health interventions which can reduce the risk of infection and death are urgently required. This article discusses the roles of vitamin D in reducing the risk of COVID-19, and how vitamin D supplementation may be a useful risk reduction measure. Vitamin D can reduce the risk of infections through a variety of mechanisms: induction of cathelicidins and defensins that can lower the rate of viral replication and decrease the concentrations of pro-inflammatory cytokines, which are responsible for induction of inflammation, injuring lining of lungs and contributing to developing pneumonia. Evidence supporting the role of vitamin D in reducing the incidence of COVID-19 includes a) winter outbreak; b) a timeframe when concentrations of 25-hydroxyvitamin D (25(OH)D) are lowest; c) a small number of cases in the southern hemisphere toward the end of summer; d) a vitamin D deficiency found to lead to acute respiratory distress syndrome (ARDS); e) and a rise in case-fatality rates with increasing age and comorbid chronic diseases, both of which are associated with lower concentrations of 25(OH)D. It is recommended that people at risk of COVID-19 consider taking 10,000 IU/d of vitamin D3 for a few weeks to rapidly increase 25(OH)D concentrations, followed by 5,000 IU/d to reduce the risk of infection. Higher doses of vitamin D3 may be useful for treating people who are infected with COVID-19. To test these guidelines, randomized controlled trials and comprehensive population studies should be performed.

Concentration of 25-hydroxyvitamin D in 246 men’s blood serum population on Wroclaw

The aim of the study was to analyze vitamin D concentrations in the blood of 246 men aged 16–89 in the period from September 2017 to February 2018. As the material for research used the results of 25(OH)D concentration determinations from the Laboratory of the Lower Silesian Medical Center DOLMED S.A. The assays were performed using the Roche Diagnostics – Cobas e410 immunochemical analyzer. Vitamin D test was used. It is the competence assay, which is based on the phenomenon of ectrochemiluminescence (ECLI). The analysis showed that the average concentration of vitamin D in the body of the studied patients was 27.7 ng/ml. 6% of men tested had a deficit, 26% had a deficiency, and 27% had hypovitaminosis. The concentration of the housing in the reference range were observed in 41% of research. The relationship between age and 25 (OH) D concentration was demonstrated. With increasing age, the concentration of vitamin D increases. In the population studied, there were no concentrations higher than 80 ng/ml. The relationship between calcidiol concentration and the month in which the study was performed was also demonstrated. The statistical analysis of the obtained data was made using Statistica version 13 and Microsoft Excel 2016.

Vitamin D intake is major determinant of the concentration of 25-hydroxyvitamin D in the serum of healthy postmenopausal women.

Vitamin D intake is major determinant of the concentration of 25-hydroxyvitamin D in the serum of healthy postmenopausal women.

Sociodemographic disparity in the nutritional status among children and adolescents in Zhejiang Province.

To explore the sociodemographic disparity in the nutritional status including BMI, vitamin D and vitamin A status among children and adolescents in Zhejiang Province. Weight, height serum 25-hydroxyvitamin D, and serum retinol were assessed in 2818 children and adolescents (6-17 years of age), using ten investigation sites in Zhejiang Province including urban and rural areas data from the China National Nutritional Study 2016-2017, which is a nationally representative cross-sectional study, to form provincial representative sample of Zhejiang Province. Sociodemographic disparity was explored. The prevalence of low weight, eutrophic, overweight and obese were 6.7%, 70.4%, 11.6%, and 11.2%, respectively. Significant difference was observed on BMI between males and females (P < 0.05), and between urban and rural areas (P < 0.05). The prevalence of vitamin D deficiency, inadequacy, normal and appropriate were 1.8%, 34.9%, 46.4% and 16.9%, respectively. Significant difference was observed on the concentration of 25-hydroxyvitamin D (25(OH)D) both between genders and between living areas (P < 0.05). The prevalence of vitamin A deficiency, marginal deficiency and appropriate were 4.5%, 24.7%, and 70.9%, respectively. Significant difference was observed on the concentration of retinol between living area (P < 0.05). Overweight and obesity, deficiency and inadequacy of vitamin D, deficiency and marginal deficiency of vitamin A were prevalent among children and adolescents. There were disparities between genders and between living areas on the nutritional status. Regional and sex-specific guidelines and public health policies for children and adolescent nutrition are needed.