BackgroundNowadays, computer users are facing musculoskeletal disorders (MSDs) and visual symptoms. Prolonged sitting in inappropriate, awkward, and static postures on the computer workstation may cause musculoskeletal disorders (MSDs). Similarly, inappropriate placement of monitors, illumination, and other factors such as prolonged usage of computers are related to visual symptoms. Objective: This study aims to evaluate the ergonomic deficiencies of computer workstations and their correlation with MSDs and visual symptoms. Methods: This study involved 271 university employees from a Bangladeshi engineering university. Ergonomic deficiencies were evaluated through direct observations and Occupational Safety and Health Administration checklists. In addition, the Nordic Musculoskeletal Questionnaire was used to assess the prevalence of MSDs and visual discomforts. Binary Logistic Regression (BLR) analysis was also used to examine the correlation between musculoskeletal symptoms and ergonomic deficiencies. Results: Results showed serious deficiencies in workstation setup, seating arrangement, monitor orientations, keyboard orientations, other input device orientations, and accessory setup. Employees reported that the MSDs in different body regions during the last 12 months including lower back (62 %), upper back (53 %), shoulders (47 %), and neck (25 %). Moreover, itchy eyes (69 %), tired eyes (83 %), and unclear vision (56.83 %) were the most common visual discomforts or visual symptoms among the participants. Results also revealed that monitor ergonomics and its orientation deficits were significantly associated with visual discomforts. Gender, job type, age, BMI, work experience, duration of computer work, and beak taking after 2 h were the independent variables reliably predicting the MSDs and visual symptoms. Conclusion: It is evident that MSDs and visual symptoms were associated with computer workstation deficiencies and other work-related factors.