Comprehensive Examination Research Articles

Relationship between perceived stress and levels of blood pressure: single center study

<p><strong>Introduction. </strong>High blood pressure (HBP) is a significant cardiovascular risk factor, with hypertension recognized as a leading cause of global mortality. This study aims to investigate the correlation between perceived stress and <br />blood pressure levels among patients diagnosed with hypertension. <br /><strong>Methods.</strong> A cross-sectional study was conducted involving 80 hypertensive patients at the Institute for Cardiovascular Diseases “Dedinje”. Comprehensive clinical examinations, including 24-hour ambulatory blood pressure monitoring (ABPM) and Perceived Stress Scale (PSS) assessments were performed. <br /><strong>Results.</strong> The results revealed a significant positive correlation between PSS scores and blood pressure levels (r = 0.65, p < 0.001), indicating that higher perceived stress is associated with elevated blood pressure. Furthermore, no statistically significant difference was found between stress levels and sex or smoking status. <br /><strong>Conclusion.</strong> These findings underscore the importance of stress management interventions in hypertension treatment, suggesting that addressing perceived stress could enhance therapeutic outcomes for patients.</p>

Investigating the Factors Affecting Customer Trust in E-Commerce

In the rapidly evolving landscape of e-commerce, the foundation of customer trust has emerged as a critical determinant of success for online businesses. This article delves into the multifaceted nature of trust in e-commerce, identifying and analyzing key factors that influence consumer confidence and loyalty. Through a comprehensive examination, we highlight the importance of website design and usability, security measures, customer reviews and testimonials, customer service and support, and the impact of brand reputation and influencer endorsements. The discussion extends to actionable strategies for e-commerce businesses to build and maintain customer trust, emphasizing the need for continuous improvement and adaptation to changing consumer expectations and technological advancements. Real-life case studies provide insights into successful and unsuccessful attempts to foster trust online, underscoring the lessons learned. The article concludes by reflecting on the dynamic nature of trust in e-commerce, urging businesses to stay vigilant and proactive in creating a trustworthy shopping environment. This analysis serves as a valuable resource for e-commerce businesses aiming to navigate the complexities of building trust in the digital age.

Clinical characteristics, progression patterns and treatment outcomes in microsporidial keratoconjunctivitis: a prospective study in Thailand.

To assess the clinical characteristics, progression patterns, and treatment outcomes of microbiologically confirmed microsporidial keratoconjunctivitis (MKC). This prospective cross-sectional study included patients with superficial punctate epithelial keratitis clinically suspected of MKC. Comprehensive slit-lamp examinations were conducted, and corneal scraping was performed for Gram-chromotrope staining and polymerase chain reaction (PCR) analysis. A standardized questionnaire gathered demographic data, clinical features, and risk behaviors. Treatment regimens and corneal findings, including medication and frequency, were documented at each visit. PCR confirmed the diagnosis of MKC in 96 out of 117 eyes (82.1%), identifying Vittaforma corneae in 93.7% of cases, Microsporidium sp. in 4.2%, and Encephalitozoon hellem in 2.1%. All cases exhibited similar characteristics and pattern of progression, including elevated epithelial lesions in diffused distribution (34.1%), typical target lesions (31.3%), and subepithelial infiltrations (41.7%). Treatment with topical moxifloxacin, with or without oral albendazole, followed by topical steroids for subepithelial infiltrates, led to clinical improvement within approximately two weeks, with 52% of patients achieving complete recovery. This study identifies key clinical features and progression patterns in MKC. Topical fluoroquinolone monotherapy, or its combination with topical steroids or oral albendazole, results in favorable visual outcomes without corneal scarring. These insights may inform and enhance clinical management of MKC.

An analytical study of ophthalmological manifestations in hematological malignancies in a tertiary care centre, India

: The objective of the study was to describe the ophthalmological manifestations associated with various haematological malignancies at a tertiary care centre. This analytical cross-sectional study was conducted with 60 patients diagnosed with various types of haematological malignancies, including leukaemia, lymphomas, and multiple myeloma. Patients who had ocular media opacities which precluded fundus view like dense corneal opacity, cataracts, absolute glaucoma, and systemic conditions like hypertension, and diabetes were excluded from this study. All 60 patients underwent a comprehensive ocular examination and the collected data were analysed using IBM SPSS software version 25. Out of the 60 patients with haematological malignancies, 27 (45%) exhibited ocular features. Ocular involvement was more common in males (63%) and in the paediatric age group (1-10 years) (100%). Ocular findings were prevalent in leukaemia patients especially with chronic myeloid leukaemia showing the highest incidence (80%), compared to lymphomas and multiple myeloma. The most common ocular manifestations were in the posterior segment, such as retinal haemorrhages and Roth spots, observed across all age groups. Neurological involvement, specifically III and VI cranial nerve palsy, were also notable. At the time of presentation, the majority of patients with ocular features had good visual acuity, ranging from 6/6 to 6/9 (43%). With advancements in diagnostic and therapeutic methods, the survival rates for patients with haematological malignancies have considerably improved. This has led to an increase in the variability of ocular presentations. Consequently, routine ocular examination is essential at the time of diagnosis of haematological malignancies to prevent vision-threatening complications and improve the overall quality of life for these patients.

Watch your eyes: Workplace ocular hazards presenting at a tertiary eye care centre in south India

: Eye injuries can majorly affect individuals by reducing quality of life. In this study we report the prevalence of ocular trauma and the proportion of vision loss due to trauma presenting in tertiary care center.To evaluate the prevalence of occupational ocular injuries and the degree of visual loss in patients attending a tertiary eye care hospital in South India.The study was a retrospective observational study, which included patient’s aged 16 years and older attending Outpatient department of a tertiary eye care hospital following ocular trauma at workplace. The study took place across a six-month duration. A detailed demographic profile and history of the patient was documented. Patient underwent comprehensive ocular examination and further orbital imaging if necessary. The ocular injuries were classified using the Birmingham Eye Trauma Terminology System.This study included 210 patients, with a mean age group of 21-30 years. Men had a more than five-times higher rate of eye injury at work than women; with the most common mode of injury being metallic foreign body (75%). The predominant ocular injuries were categorized as closed globe injuries with 83 % incidence. 67% of the patients needed surgical intervention.Most work-related eye injuries often result from inadequate eye protection, with the majority of affected patients being young men, which highlights the importance of implementing preventive measures early in their careers. This study underscores the need of awareness and prevention programs for eye protection at workplace.

The renal-retinal connection - The eye in chronic kidney disease

Chronic kidney disease (CKD) is linked with several systemic complications, including ocular manifestations that can adversely affect quality of life. The prevalence and types of ocular changes in CKD patients, particularly their correlation with disease stages, are not well-documented. This study aims to evaluate the ocular manifestations in CKD and their association with different stages of the disease.A cross-sectional study on 70 CKD patients revealed a high prevalence of ocular abnormalities, including diabetic retinopathy, hypertensive retinopathy, and age-related macular degeneration. These conditions often worsened as CKD progressed, emphasizing the need for regular eye exams in CKD patients to prevent vision loss and address complications promptly. To investigate the prevalence and types of ocular manifestations in patients with chronic kidney disease (CKD) and to analyse their correlation with the stages of CKD. This cross-sectional, descriptive, non-interventional, hospital-based study was conducted over four months from May 2024 to August 2024 in the Department of Nephrology. Seventy patients with diagnosed CKD, aged 18 years and older, were included. Exclusion criteria were patients with reversible causes of renal failure, those who had undergone renal transplantation, and those who did not consent. A comprehensive ocular examination was performed on 140 eyes from the enrolled patients, assessing anterior and posterior segment findings, including visual acuity, eyelid conditions, conjunctival status, lens status, and fundus examination. The study included 38 males (54.3%) and 32 females (45.7%) with a mean age of 57 years. Hypertension and diabetes mellitus were the most common comorbidities, present in 71.4% and 64.3% of patients, respectively. Visual acuity deteriorated with advancing stages of CKD, with significant impairment and blindness observed predominantly in CKD stages 4 and 5. Anterior segment examination revealed that 62% of eyes had immature cataracts, and 38% were pseudophakic. Fundoscopic examination showed that 51.4% of patients had abnormal findings, including non-proliferative and proliferative diabetic retinopathy, hypertensive retinopathy, age-related macular degeneration, and glaucomatous disc changes. The severity of fundus abnormalities increased with the progression of CKD, highlighting a strong correlation between the degree of renal dysfunction and ocular health. Ocular manifestations are common in CKD patients and correlate with the severity of kidney disease. The prevalence of both anterior and posterior segment abnormalities underscores the need for regular ophthalmic evaluations in CKD patients to prevent vision loss and manage ocular complications early.

Technical Properties of the ClassMaps Survey

More than a decade after two comprehensive examinations of the technical properties of the ClassMaps Survey (CMS), this study reexamined the structure and internal consistency of the scale and, for the first time, examined its measurement invariance across gender and school levels. Participants were 1,083 elementary and middle level students from a diverse northeastern school district. Students had completed the CMS as part of their district’s evaluation of its bullying prevention program; the deidentified results were shared with researchers with approval of the district school board. Results of a seven-factor confirmatory factor analysis showed that all items loaded on their respective subscale, exhibited adequate model fit (CFI = 0.91; RMSEA = .04; SRMR = .07), and communicated good internal consistency (α = .78 to .92). Partial strong invariance was demonstrated across gender groups and school level groups. Gender effects, school level effects, and gender by school level interactions were demonstrated for some subscales. The collective results support the use of the CMS to support planning and refining classroom routines and practices that promote students’ classroom autonomy and effective relationships.

Diarenofuran[b]-fused BODIPYs: One-Pot SNAr-Suzuki Synthesis and Properties.

We present a streamlined methodology that integrates regioselective tetrahalogen-BODIPY and o-hydroxyphenylboronic acid in a one-pot process, leveraging SNAr nucleophilic substitution in conjunction with Suzuki coupling to afford diarenofuran [b]-fused BODIPYs (DBFB1-9) with commendable yields (85-95%) and short reaction times (0.5-1.0 h). X-ray structure analyses of DBFB5,7-9 elucidate that these diarenofuran[b]-fused BODIPYs adopt a "butterfly" conformation, maintaining a highly rigid planarity. A comprehensive examination of the spectroscopic and electrochemical properties of these diarenofuran[b]-fused BODIPY derivatives, incorporating various substituents, reveals intriguing characteristics, including pronounced absorption and emission in the near-infrared region (NIR), elevated fluorescence quantum yields (ΦF = 75-89% in dichloromethane), and tunable HOMO-LUMO levels. Remarkably, compared to DBFB1-8, DBFB9 possesses a large extended π-conjugated system, resulting in significant red shifts in its absorption and emission maxima, reaching 623 and 635 nm, respectively, and a reduced HOMO-LUMO gap. Despite this substantial structural expansion, DBFB9 maintains a surprisingly high fluorescence quantum yield (ΦF = 80%), underscoring its exceptional photophysical performance and strong potential for applications requiring efficient NIR emission and robust fluorescence retention.

AI-based feedback tools in education: A comprehensive bibliometric analysis study

This bibliometric analysis offers a comprehensive examination of AI-based feedback tools in education, utilizing data retrieved from the Web of Science (WoS) database. Encompassing a total of 239 articles from an expansive timeframe, spanning from inception to February 2024, this study provides a thorough overview of the evolution and current state of research in this domain. Through meticulous analysis, it tracks the growth trajectory of publications over time, revealing the increasing scholarly attention towards AI-driven feedback mechanisms in educational contexts. By describing critical thematic areas such as the role of feedback in enhancing learning outcomes, the integration of AI technologies into educational practices, and the efficacy of AI-based feedback tools in facilitating personalized learning experiences, the analysis offers valuable insights into the multifaceted nature of this field. By employing sophisticated bibliometric mapping techniques, including co-citation analysis and keyword co-occurrence analysis, the study uncovers the underlying intellectual structure of the research landscape, identifying prominent themes, influential articles, and emerging trends. Furthermore, it identifies productive authors, institutions, and countries contributing to the discourse, providing a detailed understanding of the collaborative networks and citation patterns within the community. This comprehensive synthesis of the literature serves as a valuable resource for researchers, practitioners, and policymakers alike, offering guidance on harnessing the potential of AI technologies to revolutionize teaching and learning practices in education.

China's Patient Capital in Africa: Navigating Long-term Investments and Economic Cooperation

Abstract: In this article, it will undertake a comprehensive examination of "patient capital" within the context of China's foreign direct investment (FDI) in Africa, introducing the concept and its distinctive characteristics. This analysis is framed against the backdrop of China's evolving economic and trade relationships with other nations. Firstly, it will provide a brief literature review outlining the essence of patient capital and its application in China's FDI strategies. Subsequently, this paper will delve into the historical trajectory of China's direct investment in Africa, examining shifts from state-owned enterprises (SOEs) to private enterprises and their subsequent impacts on African economies. This section underscores the emergence of long-term oriented "patient capital" approaches, challenging perceptions of China's investments as purely altruistic. Furthermore, it will analyze the structural composition of China's investments in Africa, emphasizing the strategic focus on long-term gains and relationship-building. This analysis sheds light on the transformational nature of China's engagement in Africa, fostering sustainable development and enhancing African countries' autonomous production capacities. To contextualize China's approach, this paper will compare it with that of the United States, exploring differences in investment strategies, motivations, and their respective outcomes on African nations. Utilizing case studies, it will assess the multi-faceted impacts of these investments on African economies and their contributions to China's domestic economic advancement. This comparative analysis underscores the practical significance of China-Africa cooperation, highlighting a historical opportunity for mutual benefit and win-win outcomes. By emphasizing sustainable development and capacity-building, China's FDI in Africa presents a compelling narrative of shared prosperity and growth.

Nursing doctoral students' experiences of the courses and comprehensive examinations in China: a mixed-methods study.

Understanding the experiences of doctoral students regarding courses and comprehensive examinations is crucial to enhance the quality of doctoral programs. Scarce information is available on the experiences of nursing doctoral students on the courses and comprehensive examinations in China. The purpose of this study was to understand the nursing doctoral students' experiences on courses and comprehensive examinations of the doctoral program at Xiangya School of Nursing, Central South University, and propose improvements. A convergent parallel mixed methods design adhering to EQUATOR guidelines for Good Reporting of a Mixed Methods Study. A total of 20 doctoral students were recruited through purposive sampling in September 2022 from the Xiangya School of Nursing, Central South University. They completed the self-designed questionnaire on demographic data, course, and comprehensive examination evaluations and participated in focus groups. Quantitative data were analyzed using descriptive statistics. Qualitative data were recorded and transcribed for content analysis. Regarding course experience, doctoral students preferred in-person lectures (80%) and group discussions (60%) for specialized compulsory courses. They were satisfied with the course novelty (85%), difficulty (75%), and practicality (85%), and half of the students found the credit allocation reasonable. The qualitative findings revealed their preference for appropriate and blended teaching methods, needs for additional interdisciplinary courses, statistics, and inter-school collaborative courses, accessible Nursing Philosophy and Theory courses, and high proportion of compulsory course credits. Regarding comprehensive examination experience, only 20% considered it necessary, and qualitative data revealed that they prefer more distinctive and effective contents; moreover, students were confused about the purpose of comprehensive examinations. The triangulation of quantitative and qualitative data demonstrated relative convergence. These findings illustrated nursing doctoral students' experiences on courses and comprehensive examinations and provide suggestions on improving the courses and comprehensive examinations in China and possibly in the world. This study contributes to improving the quality of nursing doctoral programs in China and can serve as valuable guidance for nursing doctoral programs with similar educational environment around the world that are undergoing course and comprehensive examination reform.

Unveiling Trends, Themes and Collaborative Networks of Research on Sustainable Development Goals in India: A Bibliometric Analysis

The goal of global sustainability has acquired substantial attention as a result of the United Nation’s Sustainable Development Goals (SDGs), which serves as a comprehensive framework for addressing major societal and environmental concerns. This study applies bibliometric analysis to assess the landscape of research articles on the SDGs. The study aims to uncover significant trends, thematic areas, influential authors, and collaborative networks within the discipline through a comprehensive examination of an extensive body of scholarly literature. The analysis entailed gathering data from prominent academic database Scopus, spanning a specific time frame. Utilizing bibliometric technique, the study assesses patterns in publication, citation, and collaboration to provide a comprehensive understanding of the research landscape. The analysis dives into the frequency of SDG-related research outputs, identifies theme clusters and goal’s interconnections, and identifies significant works affecting the conversation. The results of this bibliometric investigation reveal evolving trends in SDGs research, identify emergent and established focal points, and shed light on the changing environment of research collaboration. The analysis focused on the progress made in tackling the SDGs across several academic disciplines, while also revealing gaps and potential for future research. The findings of this bibliometric analysis have significance for policymakers, researchers, and practitioners working to advance sustainable development efforts and strengthen the collective knowledge base in support of the SDGs.

The role and impact of new technologies on healthcare systems

AbstractThe integration of new technologies into healthcare systems is revolutionizing the industry by significantly enhancing the capabilities of healthcare providers, improving patient outcomes, and optimizing administrative operations. This comprehensive examination critically assesses the impact of these technologies on various stakeholders within the healthcare ecosystem, highlighting both the benefits and challenges. Emerging technologies such as artificial intelligence (AI), which refers to the simulation of human intelligence by machines, the Internet of Things (IoT), which involves interconnected devices that collect and exchange health-related data in real time, Blockchain, which is a decentralized and tamper-proof ledger technology used to securely manage patient data, and wearable devices are explored for their potential applications in diagnostics, treatment, and patient monitoring. Despite their transformative potential, these technologies also bring forth a range of limitations and challenges, including ethical concerns regarding patient privacy, legal implications of data security, and technical hurdles related to interoperability and implementation. By addressing these issues, the healthcare industry can better navigate the complexities of technological advancement, ensuring that innovation contributes positively to patient care and operational efficiency.

A review on exploring the potential of PVA and chitosan in biomedical applications: A focus on tissue engineering, drug delivery and biomedical sensors.

Polymers have been integral to the advancement of biomedicine, owing to their exceptional versatility and functionality. Among these, polyvinyl alcohol (PVA) and chitosan both natural polymers stand out for their remarkable biocompatibility, biodegradability, and unique properties. This review article provides a comprehensive examination of the diverse applications of PVA and chitosan in three pivotal areas: tissue engineering, drug delivery, and biosensors. In tissue engineering, the discussion centres on how PVA and chitosan are engineered into scaffolds that not only support cell growth and differentiation but also promote tissue regeneration by closely mimicking the extracellular matrix. These scaffolds offer the necessary mechanical strength and adaptability for various biomedical applications. For drug delivery, the article delves into the development of sophisticated controlled release systems and targeted drug carriers, highlighting the polymers' customizable properties and their mucoadhesive nature, which make them highly effective across multiple drug delivery methods. Furthermore, the potential of PVA and chitosan in biosensor technology is explored, particularly their ability to interact with biomolecules and their intrinsic conductivity attributes that are essential for creating sensitive, reliable, and biocompatible sensors for medical diagnostics. By synthesizing recent research findings and suggesting future research directions, this review underscores the versatility and critical role of PVA and chitosan in pushing the boundaries of biomedical innovation. It offers valuable insights for researchers and scientists dedicated to advancing healthcare through the application of these natural polymers.

Analysis of candidate variants in a Chinese family with monozygotic twins with keratoconus: a case report

ABSTRACT Background Keratoconus (KC) is an asymmetrical bilateral corneal ectasia, of which the pathogenesis is unknown. Moreover, genetic factors play an important role. We reported ophthalmic findings in a Chinese family with monozygotic twins with KC to describe the clinical features and identify genetic variants. Methods Comprehensive ophthalmic clinical assessments and examinations, including history, slit-lamp biomicroscopy, best-corrected visual acuity, corneal topography, anterior segment optical coherence tomography, and corneal biomechanics, were carried out on the twins and their parents. Whole-genome sequencing (WGS) was performed to identify variants in this family. SIFT, PolyPhen2, MutationTaster, and CADD were used to predict the effect of amino acid substitutions on the affected protein. Results The twins presented typical KC features. However, their mother did not meet the criteria for a KC diagnosis but exhibited KC subclinical manifestations. After screening, 12 potentially pathogenic variants in 10 genes were identified in both twins and emerged as candidate variants for this family. These genes included 1 previously reported KC-associated variant (ZNF469, c.4384 G>A); 8 variants in 6 KC-associated genes (GRHPR, c.337 G>A, c.862_863del; COL6A1, c.920 G>A; FLG, c.8753C>G; HSPG2, c.9503C>T; KRT82, c.1306 G>A; SCN9A, c.5702_5706del, c.641 G>A); and 3 variants in 3 non-KC-associated genes (PDE6G, c.6C>A; HAL, c.1724C>T; AGBL1, c.2381 G>A). Conclusions The accumulation of these potentially pathogenic variants in twins may have caused KC in these twins. These results expand the spectrum of KC candidate variants and provide a basis for further studies on KC.

Next-generation sequencing in diagnosis of monogenic cholestatic liver disorders: A single-center experience

Introduction Cholestasis in childhood is a rare clinical condition, yet a definitive diagnosis is crucial for initiating treatment of these curable diseases and preventing related morbidity and mortality. The most common cause of infant cholestasis is biliary atresia (25-40%), followed by monogenic cholestatic diseases (25%), metabolic diseases (20%), and cryptogenic cholestasis. This study focuses on assessing the clinical utility of next-generation sequencing (NGS) panels, including clinical exome sequencing and whole exome sequencing, in diagnosing cholestatic diseases when the etiology cannot be elucidated through conventional methods. Materials and Methods We conducted a retrospective examination of pediatric patients who sought care at a single-center pediatric gastroenterology department between August 2020 and March 2022, and were diagnosed with cholestasis. A total of 36 patients underwent a thorough investigation to rule out infectious, toxic, metabolic, structural, chromosomal, and endocrine causes. Patients whose diagnoses were established through traditional investigations were excluded from the study. The remaining 14 patients underwent either whole exome sequencing or targeted next-generation sequencing methods. Results A definitive diagnosis was achieved for 12 patients, while two patients remained undiagnosed despite comprehensive genetic examinations. The most commonly encountered diseases in this cohort were progressive familial intrahepatic cholestasis, linked to mutations in the ABCB11, ATP8B1, and TJP2 genes, as well as Dubin-Johnson syndrome associated with ABCC2 mutations. Next-generation sequencing demonstrated a diagnostic accuracy of 85.7% in patients for whom a diagnosis could not be established through extensive traditional workup. Conclusion Next-generation sequencing emerges as a valuable diagnostic tool in cases of cholestasis where traditional methods fall short in providing a definitive diagnosis. Moreover, our study unveiled three previously undocumented variants in the ABCB11 [c.1165G>C; p.(Ala389Pro) and c.783+1G>A] and ABCC2 [c.4246_4247del; p.(Lys1416ValfsTer46)] genes.

Machine Learning in Genomics: Applications in Whole Genome Sequencing, Whole Exome Sequencing, Single-Cell Genomics, and Spatial Transcriptomics

The application of machine learning (ML) to genomics has transformed the process of analyzing and interpreting large-scale, complex datasets, leading to important breakthroughs in our knowledge of biological systems. This review provides a comprehensive overview of ML applications in key genomic areas: Whole Genome Sequencing (WGS), Whole Exome Sequencing (WES), single-cell genomics, and spatial transcriptomics. In WGS and WES, ML techniques are employed for variant calling, genome-wide association studies, rare variant analysis, and the prediction of pathogenicity. In single-cell genomics, ML facilitates clustering, trajectory inference, and cell type identification, while in spatial transcriptomics, it aids in deciphering spatial patterns of gene expression and tissue heterogeneity. This review further explores the application of ML in related omics fields, including proteomics, transcriptomics, metagenomics, epigenomics, and microbiome research. These applications encompass protein structure prediction, functional annotation, microbial community profiling, and the analysis of epigenetic modifications. We address the challenges caused by high dimensionality, variability in the data, and the requirement for interpretable machine learning models when dealing with genomic data. Emerging technologies like explainable AI and federated learning are highlighted for their potential to address these challenges. Additionally, the review addresses ethical considerations, data privacy issues, and the necessity for standardized protocols in ML applications. This comprehensive examination underscores the transformative impact of ML in genomics and highlights its potential to drive future innovations in personalized medicine and biological research.

Worldwide burden of retinoblastoma from 1990-2021.

To report the global, regional, and national burden of retinoblastoma between 1990 and 2021, by age, sex, and sociodemographic index (SDI). We leveraged the Global Burden of Disease 2021 Study to elucidate the epidemiological landscape of retinoblastoma, encompassing prevalence, incidence, mortality, and disability-adjusted life years (DALYs) across 204 nations and territories spanning the period from 1990 to 2021. The SDI was employed to evaluate the interplay between socio-economic development and the burden of retinoblastoma. In 2021, global estimates unveiled 57,333 prevalent cases of retinoblastoma, yielding 6,274 incident cases, 2,762 deaths, and 243,204 DALYs. Globally, the age-standardized prevalence, incidence, mortality, and DALY rates for retinoblastoma in 2021 were 0.86, 0.09, 0.04, and 3.65 per 100,000 population, respectively. Tokelau, Kenya, and Portugal demonstrated the highest age-standardized prevalence and incidence rates of retinoblastoma in 2021. The global prevalence of retinoblastoma peaks among children aged 2 to 4 years and subsequently declines with increasing age. At the regional level, the correlation between SDI and age-standardized prevalence rates for retinoblastoma manifested a V-shaped pattern. This comprehensive examination of retinoblastoma epidemiological trends underscores the imperative for heightened vigilance and more efficacious therapeutic interventions, especially within resource-limited environments. The findings accentuate the need for targeted strategies to address the disparate burden of retinoblastoma across diverse socioeconomic landscapes.

Microgravity and Human Body: Unraveling the Potential Role of Heat-Shock Proteins in Spaceflight and Future Space Missions

In recent years, the increasing number of long-duration space missions has prompted the scientific community to undertake a more comprehensive examination of the impact of microgravity on the human body during spaceflight. This review aims to assess the current knowledge regarding the consequences of exposure to an extreme environment, like microgravity, on the human body, focusing on the role of heat-shock proteins (HSPs). Previous studies have demonstrated that long-term exposure to microgravity during spaceflight can cause various changes in the human body, such as muscle atrophy, changes in muscle fiber composition, cardiovascular function, bone density, and even immune system functions. It has been postulated that heat-shock proteins (HSPs) may play a role in mitigating the harmful effects of microgravity-induced stress. According to past studies, heat-shock proteins (HSPs) are upregulated under simulated microgravity conditions. This upregulation assists in the maintenance of the proper folding and function of other proteins during stressful conditions, thereby safeguarding the physiological systems of organisms from the detrimental effects of microgravity. HSPs could also be used as biomarkers to assess the level of cellular stress in tissues and cells exposed to microgravity. Therefore, modulation of HSPs by drugs and genetic or environmental techniques could prove to be a potential therapeutic strategy to reduce the negative physiological consequences of long-duration spaceflight in astronauts.

Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley.

Existence of potent in vitro regeneration system is a prerequisite for efficient genetic transformation and functional genomics of crop plants. In this study, two contrasting cultivars differencing in their in vitro regeneration efficiency were identified. Tissue culture friendly cultivar Golden Promise (GP) and tissue culture resistant DWRB91(D91) were selected as contrasting cultivars to investigate the molecular basis of regeneration efficiency through multiomics analysis. Transcriptomics analysis revealed 1487 differentially expressed genes (DEGs), in which 795 DEGs were upregulated and 692 DEGs were downregulated in the GP-D91 transcriptome. Genes encoding proteins localized in chloroplast and involved in ROS generation were upregulated in the embryogenic calli of GP. Moreover, proteome analysis by LC-MS/MS revealed 3062 protein groups and 16,989 peptide groups, out of these 1586 protein groups were differentially expressed proteins (DEPs). Eventually, GC-MS based metabolomics analysis revealed the higher activity of plastids and alterations in key metabolic processes such as sugar metabolism, fatty acid biosynthesis, and secondary metabolism. TEM analysis also revealed differential plastid development. Higher accumulation of sugars, amino acids and metabolites corresponding to lignin biosynthesis were observed in GP as compared to D91. A comprehensive examination of gene expression, protein profiling and metabolite patterns unveiled a significant increase in the genes encompassing various functions, such as ion homeostasis, chlorophyll metabolic process, ROS regulation, and the secondary metabolic pathway.