Comprehensive Ecological Risk Assessment Research Articles

Distribution and risk assessment of microplastics in water, sediment and brine shrimps in a remote salt lake on the Tibetan Plateau, China

Microplastics (MPs) are pervasive environmental contaminants that have infiltrated even the most remote ecosystems. Despite their widespread distribution, the transfer patterns and impacts of MPs in remote lakes remain poorly understood. This study aimed to address the knowledge gap regarding the pathways and consequences of MP pollution in these isolated environments. Focusing on Kyêbxang Co, a remote salt lake in Tibet, this study investigated the transfer patterns, sources and ecological impacts of MPs, providing insights into their mobility and fate in pristine ecosystems. Water, sediment and biota (brine shrimp) samples from Kyêbxang Co, collected during the summer of 2020, were analyzed using µ-Raman spectroscopy to determine MP abundances, polymer types and potential sources. Findings indicated significant MP contamination in all examined media, with concentrations highlighting the role of runoff in transporting MPs to remote locations. The majority of detected MPs were small fragments (<0.5 mm), constituting over 93 %, with polypropylene being the predominant polymer type. The presence of a halocline may slow the descent of MPs, potentially increasing the exposure and ingestion risk to brine shrimp. Despite the currently low ecological risk estimated for MPs, this study underscores the need for long-term monitoring and development of a comprehensive ecological risk assessment model for MPs.

Combined effects of toxic Microcystis aeruginosa and high pH on antioxidant responses, immune responses, and apoptosis of the edible freshwater bivalve Corbicula fluminea

Due to increasing anthropogenic perturbation and water eutrophication, cyanobacterial blooms (CYBs) have become a global ecological and environmental problem. Toxic CYBs and elevated pH are considered to be the two key stressors associated with eutrophication in natural waters, particularly in the event of CO2 depletion induced by dense blooms. However, previous research has been focused on investigating the impacts of toxic CYBs or pH changes in isolation, whereas the interactive effects of such stressors on edible bivalves that inhabit CYB waters still lack information. In this study, the combined effects of toxic Microcystis aeruginosa and pH shifts on the antioxidant responses, immune responses, and apoptosis of the edible freshwater bivalve Corbicula fluminea were explored. The results showed that the activity of antioxidant enzymes was significantly impacted by the interactive effects between toxic M. aeruginosa exposure and time course, yet pH shifts showed no significant effects on the activities of these antioxidant enzymes, implying that the antioxidant response in C. fluminea was mainly triggered by toxic M. aeruginosa exposure. Toxic M. aeruginosa also induced an increased production of reactive oxygen species and malondialdehyde in treated clams, particularly under high pH settings. The elevated lysosomal enzyme activity helped C. fluminea defend against toxic M. aeruginosa exposure under high pH conditions. The principal component analysis (PCA) and the integrated biomarker response (IBR) results suggested that the treated clams were subjected to the elevated toxicity of toxic M. aeruginosa in conditions of high pH. The heat shock proteins-related genes might be triggered to resist the oxidative damage in treated clams. Moreover, the upregulation of TNF and casp8 genes indicated the potential activation of the caspase8-mediated apoptotic pathway through TNF receptor interaction, potentially resulting in apoptosis. The TUNEL assay results further confirmed that apoptosis appeared in treated clams. These findings improve our understanding of the combined toxicological effects of harmful algae and pH shifts on bivalves, which will provide insights into a comprehensive ecological risk assessment of toxic CYBs to edible bivalve species.

Combining chemical analysis and toxicological methods to access the ecological risk of complex contamination in Daye Lake

As one of the nine primary non-ferrous metal smelting bases in China, Daye Lake basin was polluted due to diverse human activities. But so far the pollution status and related ecological risks of this region have not been detailly investigated. In current study, pollutants including heavy metals, polycyclic aromatic hydrocarbons (PAHs) and organochlorine pesticides (OCPs) in eight sediment samples from Daye Lake were quantified. 18S rRNA gene sequencing was employed to profile the nematode community structure within these sediments. Model organism Caenorhabditis elegans (C. elegans) were further applied for a comprehensive ecological risk assessment of Daye Lake. Notably, Cadmium (Cd) was identified as a key driver of ecological risk, reaching an index of 1287.35. At sample point S4, OCPs particularly p,p′-DDT, displayed an extreme ecological risk with a value of 23.19. Cephalobidae and Mononchida showed strong sensitivity to pollutant levels, reinforcing their suitability as robust bioindicators. The composite pollutants in sampled sediments caused oxidative stress in C. elegans, with gene Vit-2 and Mtl-1 as sensitive biomarkers. By employing the multiple analysis methods, our data can offer valuable contributions to environmental monitoring and health risk assessment for composite polluted areas.

Ecological risk assessment of commonly used antibiotics in aquatic ecosystems along the coast of China

The consistent input of antibiotics into aquatic environments may pose risks to various creatures and ecosystems. However, risk assessment of pharmaceuticals and personal care products (PPCPs) in aquatic environments is frequently limited by the lack of toxicity data. To investigate the risk of commonly used antibiotics to various aquatic creatures, we focused on the distribution patterns and temporal dynamics of antibiotics in the coastal estuary area of China and performed a comprehensive ecological risk assessment for four antibiotics: erythromycin (ERY), tetracycline (TCN), norfloxacin (NOR) and sulfamethoxazole (SMX). An interspecies correlation estimation (ICE)-species sensitivity distribution (SSD) combined model was applied to predict the toxicity data of untested aquatic species, and an accurate ecological risk assessment procedure was developed to evaluate the risk level of PPCPs. The results of risk quotient assessments and probabilistic risk assessments (PRAs) suggested that four objective antibiotics in the Chinese coastal estuary area were at a low risk level. These antibiotics posed a high risk in antibiotic-related global hot spots, with probabilistic risk values for ERY, NOR, SMX, and TCN of 81.33 %, 27.08 %, 21.13 %, and 15.44 %, respectively. We applied an extrapolation method to overcome the lack of toxicity data in ecological risk assessment, enhanced the ecological reality of water quality criteria derivation and reduced the uncertainty of risk assessment for antibiotics.

Comprehensive Assessment of Heavy Metal Pollution and Ecological Risk in Lakes of Bangalore, Karnataka, India

The contamination of water and sediment by heavy metals poses significant challenges to freshwater ecosystems, agriculture, and aquaculture. This study focused on assessing heavy metal pollution in Ulsoor and Agara Lakes, prominent freshwater reservoirs in Bengaluru utilized for aquaculture. The presence of heavy metals such as Cd, Cu, Zn, Cr and Pb levels were analyzed in water and sediment samples of both lakes over a period of one year (from August 2021 to July 2022), using an Atomic Absorption Spectrometer. Pollution indices (CF, Igeo, PLI, ERI) were computed for sediment, revealing higher metal concentrations compared to water. Correlation analysis identified strong associations between Zn and Cu, Pb and Zn in water, and Cu and Cd in sediment, suggesting common pollution sources. While sediment showed relative safety based on PLI, Igeo, and ERI, CF indicated an unpolluted to moderately polluted status. In conclusion, this study revealed that most metals in the water exceeded permissible limits, highlighting the need for effective remediation measures.

Ecological risk assessment under the PSR framework and its application to shallow urban lakes.

Shallow urban lakes are naturally vulnerable to ecosystem degradation. Rapid urbanization in recent decades has led to a variety of aquatic problems such as eutrophication, algal blooms, and biodiversity loss, increasing the risk to lake-wide ecological sustainability. Instead of a simple binary assessment of ecological risk, holistic evaluation frameworks that consider multiple stressors and receptors can provide a more comprehensive assessment of overall ecological risk. In this study, we analyzed a combined dataset of government statistics, remote sensing images, and 1year of field measurements to develop an index system for urban lake ecological risk assessment based on the pressure-state-response (PSR) framework. We used the developed ecological safety index (ESI) system to evaluate the ecological risk for three urban lakes in Jiangsu Province, China: Lake Yangcheng-LYC, Lake Changdang-LCD, and Lake Tashan-LTS. LYC and LTS were classified as "mostly safe" and "generally recognized as safe," respectively, while LCD was assessed as having "potential ecological risk." Our data suggest that socioeconomic pressure and aquatic health are the two main factors affecting the ecological risk in both LYC and LCD. The ecological risk of LTS could be improved more effectively if regional management plans are well implemented. Our study highlights the pressure of external wastewater loading, low forest-grassland coverage, and lake shoreline damage on the three selected urban lakes. The findings of this study can inform watershed management for lake ecosystem restoration and environmental sustainability.

Comprehensive assessment of health and ecological risk of cadmium in agricultural soils across China: A tiered framework

Soil cadmium (Cd) contamination has been increasingly serious in agricultural land across China, posing unexpected risks to human health concerning crop safety and terrestrial ecosystems. This study collected Cd concentration data from 3388 soil sites in agricultural regions. To assess the Cd risk to crop safety, a comprehensive sampling investigation was performed to develop reliable Soil Plant Transfer (SPT) model. Eco-toxicity tests with representative soils and organism was conducted to construct the Species Sensitivity Distribution (SSD) for ecological risk assessment. Then, a tiered framework was applied based on Accumulation index, deterministic method (Hazard quotient), and probabilistic assessment (Monte Carlo and Joint Probability Curve). The results revealed the widespread Cd enrichment in agricultural soils, mainly concentrated in Central, Southern, and Southwest China. Risk assessments demonstrated the greater risks related to crop safety, while the ecological risks posed by soil Cd were manageable. Notably, agricultural soils in southern regions of China exhibited more severe risks to both crop safety and soil ecosystem, compared to other agricultural regions. Furthermore, tiered methodology proposed here, can be adapted to other trace elements with potential risks to crop safety and terrestrial ecosystem.

Examining Relationships between Regional Ecological Risk and Land Use Using the Granger Causality Test Applied to a Mining City, Daye, China

Land use changes are an important factor contributing to the increasingly severe deterioration of the ecological environment. Therefore, regional analyses of land use and ecological risk should be developed for the restoration of ecological functions. In this study, a comprehensive ecological risk assessment was constructed on a regional scale and applied to Daye, a traditional mining city in China. Cointegration analyses and Granger causality tests were used to explore the complex relationship between land use and ecological risks in the study area from 2007 to 2021. The results show a long-term and stable relationship between land use changes in different sub-regions and ecological risks, albeit with distinct Granger causality relationships. This research presents the development trend of the relationship between land use change and ecological risks in a mining city, from rapid economic growth to economic restructuring and full-region ecological governance.

Effect of Butyl Paraben on Oxidative Stress in the Liver of Mauremys sinensis.

Butyl paraben (BuP) has been widely used as a preservative in the cosmetics, food, and medicine industries. Recently, it has become a new pollutant and has attracted much attention. In order to evaluate the toxic effect of BuP on aquatic animals, Chinese striped-neck turtles (Mauremys sinensis) were exposed to BuP solutions with different concentrations of 0, 5, 50, 500, and 5000 µg/L for 20 weeks. The results showed that with an increase in BuP concentration, the activity of antioxidant enzymes (SOD, CAT and GSH-PX) in liver decreased. The expression of key genes in the Nrf2-Keap1 signal pathways first increased and then decreased, while the expression of the HSP70 and HSP90 genes increased. In addition, the liver had an inflammatory reaction. The expression of the BAFF and IL-6 genes increased and then decreased with an increase in BuP concentration, while the expression of P50 and P65 increased significantly. Oxidative stress induced apoptosis, and the expression of pro-apoptosis genes (BAX, cytc, Caspase3 and Caspase9) increased, while the expression of the anti-apoptosis gene Bcl2 decreased. The results provide an important reference for the comprehensive ecological and health risk assessment of environmental BuP.

Thoracic limb activity of Simocephalus vetulus and its descendants is shaped by the combination of delafloxacin and calcium

Due to its robust antibacterial activity in acidic environments and effectiveness against old fluoroquinolone-resistant bacterial strains, the use of delafloxacin (DFX) is projected to increase in the coming years. Here, we investigated the effect on the thoracic limb rate (TLR) of the water flea Simocephalus vetulus under the combined exposure of DFX (0, 100, 200, and 400 µg L−1) and calcium (Ca) (26 and 78 mg L−1) using Bayesian statistics-based linear models. The findings showed unexpected increases and decreases in the TLR of maternal S. vetulus exposed to DFX in the 26 mg Ca L−1. In the 78 mg Ca L−1 condition, we found a clearer decrease in TLR during DFX treatments. In addition, in the 26 mg Ca L−1, elusive changes in the TLR of neonatal S. vetulus whose mothers were exposed to DFX were observed, whereas the reduced TLR of the neonate, whose mother was exposed to 400 µg DFX L−1, was apparent in the higher Ca condition. While the elevated Ca content did not appear to have a clear effect on organisms’ TLR, it acted as a synergist in conjunction with DFX, leading to a reduction in TLR, the underlying mechanism of which remains poorly understood. The results of our study indicate that DFX may pose an ecological risk as a xenobiotic; therefore, taking into account the abundance of Ca in specific aquatic environments, a comprehensive ecological risk assessment should be conducted. Moreover, the inclusion of Bayesian statistics in ecotoxicological studies is highly recommended to improve data analysis and interpretation.

Effects of nano/microplastics on the growth and reproduction of the microalgae, bacteria, fungi, and Daphnia magna in the microcosms

Over the past 20 years, nano/microplastics have become a prominent environmental concern of the public and scientific community. The difference between model microplastic particles used in the laboratory with the MPs that exist in the environment and the low ecological relevance of the data obtained from single species experiments are two knowledge gaps that must be overcome before a comprehensive ecological risk assessment on MPs can be conducted. Therefore, this study examined the effects of 14 plastic particles and 6 fiber materials on the growth of microorganisms in single species and microcosm exposure experiments. The statistical analysis results of over 300 experimental data revealed that higher concentrations and smaller particle sizes of MPs led to lower growth rates of Chlorella spp. Fibrous MPs inhibited the growth of Chlorella spp. less than fragmented MPs. All the types of MPs inhibited the growth of Euglena which has no cell wall protection. Aerobic Bacillus subtilis were inhibited by MPs more than facultative anaerobic bacteria, and yeast. The existence of PS spheres caused a negative effect on the growth and reproduction of the parental D. magna, which in turn led to a negative effect on the development of the progeny. These findings demonstrated that MPs selectively inhibit or promote the growth of different species in the same habitat, which means that the presence and increase of MPs have the potential to change the species structure in natural ecosystems.

Study effect and mechanism of ofloxacin and levofloxacin on development of Rana nigromaculata tadpoles based on the hypothalamus-pituitary-thyroid axis

Excessive antibiotics transferred into the aquatic environment may affect the development of amphibians. Previous studies on the aquatic ecological risk of ofloxacin generally ignored its enantiomers. The purpose of this study was to compare the effects and mechanisms of ofloxacin (OFL) and levofloxacin (LEV) on the early development of Rana nigromaculata. After 28-day exposure at environmental levels, we found that LEV exerted more severe inhibitory effects on the development of tadpoles than OFL. According to the enrichment results of differentially expressed genes in the LEV and OFL treatments, LEV and OFL had different effects on the thyroid development of tadpoles. dio2 and trh were affected by the regulation of dexofloxacin instead of LEV. At the protein level, LEV was the main component that affected thyroid development-related protein, while dexofloxacin in OFL had little effect on thyroid development. Furthermore, molecular docking results further confirmed that LEV was a major component affecting thyroid development-related proteins, including DIO and TSH. In summary, OFL and LEV regulated the thyroid axis by differential binding to DIO and TSH proteins, thereby exerting differential effects on the thyroid development of tadpoles. Our research is of great significance for comprehensive assessment of chiral antibiotics aquatic ecological risk.

Assessment and Prediction of Landscape Ecological Risk from Land Use Change in Xinjiang, China

Land use change has significant impacts on the regional and global environment; thus, in-depth research on the associated ecological risks is necessary for promoting ecological restoration and sustainable development. Xinjiang, China, is characterized by a fragile ecological environment, and this study aimed to predict the land use change in the region in 2030 under different scenarios, including natural development, ecological conservation, and urban development, by using the PLUS model based on land use data from 2000, 2010, and 2020. Based on the landscape structure of regional ecosystems, we developed a comprehensive ecological risk assessment framework by utilizing a combination of landscape disturbance index, vulnerability index, and loss index. This framework allowed us to evaluate the spatiotemporal patterns and variations of landscape ecological risks under different scenarios in 2030. The study results indicate the following: (1) During the period from 2000 to 2020, the primary landscape type in Xinjiang was unused land. However, significant changes were observed in the area of cultivated land, mainly due to the conversion of grassland and construction land. The expansion of construction land during the urbanization process resulted in a decline in ecological landscapes, such as grassland, thereby weakening the ecosystem’s stability. (2) Under different simulation scenarios, the urban development scenario primarily led to the conversion of unused land into construction land, which is beneficial for economic development. On the other hand, the ecological conservation scenario resulted in a modest increase in construction land and a transformation of unused land into forest and grassland, which aligns with the principles of sustainable development. (3) Different scenarios in 2030 result in varying degrees of changes in each landscape type in Xinjiang, with the spatial distribution characteristics of landscape ecological risks remaining similar to those observed in 2020. Notably, under the urban development scenario, the area of lowest and medium risk areas decreases significantly while the area of higher and highest risk areas increases substantially. Conversely, under the ecological conservation scenario, the area of the lowest risk areas experiences a more significant increase. (4) Overall, the spatial differences in the ecological risk of Xinjiang’s landscape are significant, with HH and LL clustering types predominating and presenting a polarization pattern. The distribution pattern is low in the north and high in the central and southern parts of the study area.

Pesticides bioassays using neotropical aquatic species: Trends during the last twenty years and future challenges in Argentina

The presence of pesticides in aquatic ecosystems is one of the most relevant stressors which biota usually face. Laboratory tests using model organisms for pesticides toxicity assessment are employed worldwide. The use of these species has been encouraged in the scientific community due to their advantageous features and their acceptation by regulatory and standardization organizations. However, non-model species as well as those belonging particular ecosystems could contribute in the laboratory-field toxicity extrapolation. In this context, this work aims on exploring the state of the ecotoxicological studies of pesticides in neotropical aquatic species, focusing on bioassays performed in Argentina over the last 20 years as a case of study. Furthermore, we analyzed the possible advantages and disadvantages of these studies, possible differential sensitivities among native and model species, and future challenges to be faced. The analysis of more than 150 publications allowed identify the chemical identity of tested compounds, organisms used for the bioassays, characteristics of the experimental designs, and the toxicity endpoints. Particularly, the studied cases showed that the tested chemicals are related to those most used in the agricultural activity in Argentina, the predilection for particular species in some taxonomic groups (e.g. amphibians), and the wide election of biochemical biomarkers in the studies. Regarding the sensitivity comparison between native and non-native species, the amount of data available indicates that there is not a clear difference beyond some particular cases. However, deeper understanding of toxic effects of pesticides on non-model species could help in a more comprehensive ecological risk assessment in different ecosystems.

Dysfunction of liver-gut axis in marine medaka exposed to hypoxia and perfluorobutanesulfonate

With objectives to explore the interactive mode on the function of liver-gut axis, adult marine medaka were exposed for 7 days to environmentally realistic concentrations of perfluorobutanesulfonate (PFBS) (0 and 10 μg/L) under normoxia or hypoxia condition. Furthermore, PFBS exposure was extended to 21 days to reveal the temporal progression in toxicity. The results showed that hypoxia exposure significantly disturbed lipid metabolism, caused oxidative damage, and induced inflammation in the livers of medaka. The composition of gut microbiota was also drastically shifted by hypoxia acute exposure. In contrast, the effect of PFBS was much milder. Hypoxia was thus the determinant of the combined toxicity. Depending on the exposure duration, a time-course recovery from PFBS innate toxicity was generally noted. Overall, the present study underlines the hypoxic and temporal variation in the dysregulation of liver-gut axis by PFBS, which is expected to support a comprehensive ecological risk assessment.

Linking ecosystem service supply and demand to landscape ecological risk for adaptive management: The Qinghai-Tibet Plateau case

Clarifying the spatiotemporal variations in ecosystem service (ES) supply and demand helps to understand natural-social coupled systems, and comprehensive landscape ecological risk (LER) assessment is the basis for risk warning. However, it is still a huge challenge to incorporate ES supply and demand into ecological adaptive management. In this study, we defined and identified ES supply and demand risk (ESSDR), and integrated it into LER assessment to develop a comprehensive ecological risk framework. Using InVEST model and multi-source data, this study explicitly quantified the spatiotemporal variations of ESSDR of soil retention (ESSDRI_SR), carbon sequestration (ESSDRI_CS), water yield (ESSDRI_WY), LER of Qinghai Province in the Qinghai-Tibet Plateau during 2010–2020. The results indicated that all ESSDRs and LER showed spatial heterogeneity. Among the ESSDR areas, the low risk areas accounted for the highest proportion, with ESSDRI_CS, ESSDRI_SR and ESSDRI_WY accounting for 4.83%, 14.84% and 12.45%, respectively. The area of very high and high LER decreased by 1.5% and 5.45% from 2010 to 2020, reaching 19.05% and 22.74%, respectively. The comprehensive ecological risk assessment showed that over 60% of Qinghai is designated as having ecological risks. However, the region with the most risk co-occurrence (risk group 4) accounted for 0.11% of Qinghai’s area. At last, adaptive suggestions were proposed for risk management and ecological conservation. This research provides and illustrates an innovative method for comprehensive ecological risk assessment, which could substantially enhance the scientific foundation on which ecological risk assessment is based and policy-making that follow compared to traditional LER framework.

Occurrence, Distribution and Ecological Risk Assessment of Contaminants in Baiyangdian Lake, China

Understanding the ecosystem risk of contaminants in the surface water of lakes in scenic areas is important for environmental management. Here, a comprehensive ecological risk assessment evaluation method was established to assess the ecological risk of 14 water quality indicators of Baiyangdian Lake in the spring, summer, and autumn. The results show that the indices of ammonia nitrogen and permanganate exceed the Class III standard limit for water quality. Baiyangdian Lake is generally in a state of mesotrophication, and some areas are slightly eutrophic; the degree of eutrophication is higher in the summer and autumn than in the spring. The lake water at the inlet is eutrophic and polluted, with an inferior water quality. Heavy metals are almost not detected in Baiyangdian Lake. The heavy metal pollution indices of Nanliuzhuang and Caiputai are slightly higher than those of other areas. The ecological risk quotient of petroleum is high. The comprehensive ecological risk of the Baiyangdian Lake is at Level 4, owing to the continuous development of aquaculture and tourism in recent years. We should start the monitoring, management, and strengthening of water quality supervision to achieve the ecological and environmental planning goals of Baiyangdian Lake.

Comprehensive Ecological Risk Assessment of Heavy Metals Based on Species Sensitivity Distribution in Aquatic of Coastal Areas in Hong Kong.

In recent decades, the ecological environment of some coastal areas in China has been seriously affected by terrestrial pollutants, and there is an urgent need for ecological risk assessment of China’s coastal environment. The assessment of heavy metal pollution in Hong Kong waters was carried out using different environmental and ecological indicators. The heavy metal contents (Cu, Pb, Zn, Cd, As, Cr, and Hg) in the near coast of Hong Kong were analyzed for two different seasons of the year 2018 (April—spring and September—autumn). We assessed the distribution and enrichment of heavy metals in the near coast of Hong Kong, and the potential biohazardous effects were assessed using the species sensitivity distribution method. The results showed that only Pb, Zn, and Hg in seawater exceeded the Class I standard. Pb, Zn, Cd, and As in organisms exceeded the standard, and no heavy metals exceeded the standard in sediments. The species sensitivity distribution method indicated that the biohazardous factor of heavy metals of the Hong Kong coast is higher in spring than in autumn, and the potential hazard ratio has the characteristics of high northwest and low southeast, which leads to its msPAF also having these characteristics. From the correlational analyses among heavy metals, we found that the pH change in seawater was related to the concentration of heavy metals, the concentration of heavy metals in seawater was proportional to the salinity of seawater, Pb and Cu were likely to have the same source, and Zn and Cd may not have the same emission sources as the other heavy metals. Overall, heavy metal contamination of seawater, sediments, and organisms near the Hong Kong coast was within acceptable limits, but the problem of heavy metal dispersion should be prevented.

Persistent impairment of gonadal development in rare minnow (Gobiocypris rarus) after chronic exposure to chlorinated polyfluorinated ether sulfonate

The delayed and persistent adverse effects caused by developmental exposure to per- and poly-fluorinated substances are of significant concern. Juvenile rare minnows (Gobiocypris rarus), were exposed to chlorinated polyfluoroalkyl ether sulfonate (Cl-PFESA) at measured medium concentrations of 86.5 μg/L, 162 μg/L and 329 μg/L, for 4 weeks followed by 12 weeks of depuration. After 4 weeks of exposure, the body weight and length of the juvenile fish were increased compared to controls. Gene expression of gnrh3, lhβ, and cyp19a was decreased, and ar and erα were upregulated. Transcriptomic analysis revealed enrichment of multiple pathways related to gonadal development. After 12 weeks of depuration, the gonadosomatic indices were decreased in female fish in a concentration-dependent manner, with a significant decrease to 59% of control in 329 μg/L group. Histological analysis found increasing numbers of degenerating oocytes and perinucleolar oocytes, and decreasing numbers of mature vitellogenic oocytes in female fish treated by Cl-PFESA. Enlarged interstitial space of the testis was observed in the exposed male fish. Gene expression levels of gnrh3, lhβ, ar, erα, and vtg were upregulated in the adult fish. Chronic developmental exposure to Cl-PFESA caused persistent effects on gonadal development of fish, highlighting the necessity of a comprehensive ecological risk assessment.

Melanin interference toxicity or transgenerational toxicity of organic UV filter ethylhexyl salicylate on zebrafish

With the improvement of human health awareness, the production and usage of sunscreens have increased dramatically, and their active ingredients, organic ultraviolet (UV) filters (OUVFs), have the potential to induce melanin abnormalities in aquatic organisms due to their UV-absorbing properties as they enter the aquatic environment directly with the washing of skin during water activities. In this paper, the melanin interference toxicity or transgenerational toxicity effects of typical OUVFs ethylhexyl salicylate (EHS) on zebrafish (Danio rerio) were investigated based on transcriptomic sequencing technology. Results showed that EHS induced significant enrichment of the melanin-related pathway cAMP signaling pathway in parental skin tissue through UV absorption, with sensitive genes identified as melanocortin 1 receptor, protein kinase A catalytic subunit beta a, calcium/calmodulin-dependent protein kinase II delta 2, adenylate cyclase 1 and G protein subunit alpha I a. qRT-PCR verification results showed that EHS may inhibit the expression of the melanin master regulator microphthalmia-associated transcription factor a (mitfa) and its induced signaling cascade mitf-tyrosinase (tyr)-dopachrome tautomerase (dct)-tyrosinase related protein 1 (tyrp1) by inducing abnormal expression of the above sensitive genes, thereby reducing melanogenesis. After reproduction, the melanin interference effect of EHS on the parents can be carried over to offsprings through maternal inheritance of abnormally expressed mitfa and parental transfer of pollutants, as evidenced by significant enrichment of melanogenesis pathway, abnormal expression of sensitive genes mitfa, tyr, dct and tyrp1b and significant decreases in melanin content and spinal melanin area. These findings revealed the specific melanin interference toxicity of OUVFs with UV-absorbing properties, facilitating a comprehensive ecological risk assessment of OUVFs and providing scientific support for the management of new pollutants.